CRISPOR

Mus musculus (mm10), chr14:54896074-54896523, forward genomic strand

Your input sequence is 450 bp long. It contains 54 possible guide sequences.
Shown below are their PAM sites and the expected cleavage position located -3bp 5' of the PAM site.
Click on a match for the PAM NGG below to show its 20 bp-long guide sequence. (Need help? Look at the CRISPOR manual)
Colors green, yellow and red indicate high, medium and low specificity of the PAM's guide sequence in the genome.

Position  0         10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       
Variants  ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................
Sequence  ATATATGTGGTCGGTTGGTTTTGTTTTCAAGTCAGAGTTTCTCTGTTTAACCCTGGCTGTACTGGAATTACCTCTATAGACCAGGCTGGCCTTAAACTCAGAATTAAACTGGGATTAGACTTCAGGTTTATACCACCATGACTGGCCTAACTTGAGCTGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACTTGGGAGACAGAGGCAGGGATTTCTGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGTTACACAGAGAAACCCTGTCTCAAAAAAACAACAAAACCAAAAAAAAACATTTCCCAAAGAATAGTAATTGCTTATTAAAACAAGTAGGTTTTGCCTGTTAAGTGTCTCTATATATCAGTAGTTGTATATTAAATGCCTTCAGAGACCAGGTAATAAACTAAAGACTGGGCAGGGGTGGGATTGTAAACTA
                                                            CCC---   −−−TGG     CCT---    CCA---   CCT---           −−−TGG        −−−AGG      CCA---       CCT---   −−−TGG   −−−TGG      CCT---  CCC---          −−−AGG         −−−AGG    CCT---              CCA---   CCA---             CCC---                  CCA---          CCC---                        −−−AGG     CCT---                                    CCT---    CCA---           −−−TGG    −−−TGG
                                                             CCT---                      −−−AGG                      −−−GGG                      CCA---              −−−GGG                       CCA---             −−−AGG           CCA---                 −−−AGG   −−−AGG               CCT---                                  CCA---                                                                                     −−−AGG             −−−GGG    −−−GGG
                                                            −−−TGG                           −−−TGG                                                  −−−TGG               −−−TGG                       −−−TGG         −−−GGG             −−−TGG                        −−−GGG                                                                                                                                                                        −−−AGG
                                                                                                                                                                                                        −−−GGG                                                                                                                                                                                                                                        −−−GGG
                                                                                                                                                                                                                                                                                                                                                                                                                                                       −−−GGG

Download for: SerialCloner (free) - ApE (free) - GenomeCompiler - Benchling - SnapGene - Geneious - Vector NTI - LaserGene - Genbank - FASTA

Predicted guide sequences for PAMs

Ranked by default from highest to lowest specificity score (Hsu et al., Nat Biot 2013). Click on a column title to rank by a score.
If you use this website, please cite our paper in NAR 2018. Too much information? Look at the CRISPOR manual.

Download as Excel tables: Guides / Guides, all scores / Off-targets / Saturating mutagenesis assistant

Position/ Strand	Guide Sequence + PAM + Restriction Enzymes + Variants Only G- Only GG- Only A-	MIT Specificity Score	CFD Spec. score $The CFD specificity score, inspired like guidescan.com, behaves like the MIT specificity score, but it is based on the more accurate CFD off-target model, from <a href='http://www.nature.com/nbt/journal/v34/n2/full/nbt.3437.html'>Doench 2016</a>, which is also used by Crispor to rank the off-targets. The CFD specificity score correlates better than the MIT score with the total off-target cleavage fraction of a guide, see <a target=_blank href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731277/'>Tycko et al, Nat Comm 2019</a> and also the <a target=_blank href='/manual/#faq'>CRISPOR manual</a>.$	Predicted Efficiency Show all scores		Outcome		Off-targets for 0-1-2-3-4 mismatches + next to PAM	Genome Browser links to matches sorted by CFD off-target score exons only chr14 only
				Doench '16	Mor.-Mateos	Out-of-Frame	Lindel
159 / fw	`TGACTGGCCTAACTTGAGCT GGG` `.................... ...` Enzymes: AluBI, SmoI, BpuEI Cloning / PCR primers	84	91	63	46	69	75	0 - 0 - 5 - 5 - 90 0 - 0 - 0 - 0 - 1 100 off-targets	4:exon:Vkorc1l1 4:intergenic:Kif20b-Gm24006 4:intron:Vstm4 4:intron:Ccdc37 2:intergenic:Tcerg1l-Mapk1ip1 4:intergenic:Gm25881-0610008F07Rik 4:intron:Macrod2 4:intron:Lmtk2 2:intergenic:Shisa6-Gm12296 4:intergenic:Slc30a8-Gm10020 4:intergenic:Slc30a10-Lyplal1 4:intron:Mob1b 4:intergenic:Isl1-Gm23198 4:intron:Tex15 4:intron:Kansl1 4:intron:4930544I03Rik 4:exon:Pikfyve 4:intergenic:4930405D11Rik-Kif2b 4:intergenic:Gm24728-Lmo4 4:intergenic:Gm25440-Dtwd2 4:intron:Gabrr2 4:intron:Mvb12b 4:intergenic:Ank1-Gm15816/Ank1 4:intergenic:1700008P02Rik-Gm22074 4:intergenic:Mir5099-Meox2 4:intergenic:Gm9801-Ndn 4:intergenic:Gm14716-Gm14713 4:exon:Wwox 4:intergenic:Gm11412-n-R5s187 4:intergenic:Vasn/Dnaja3-Coro7 4:intergenic:Urb2-Galnt2 4:intergenic:Gm10782-Slc25a48 4:intergenic:Gm25715-N6amt1 4:intron:Cdh8 4:intergenic:n-R5s30-Rbfox1 4:intergenic:Mdfic-Tfec 4:intergenic:Gm9726/mmu-mir-8099-2-Gm26055 4:exon:Zbtb20 4:exon:Fitm2 4:exon:Cubn 4:intergenic:Gm22315-Gm16551/Gm20649 4:intron:Kcnn3 4:intron:CN725425 4:intergenic:Lancl1-Cps1 4:intron:Wisp1 2:intergenic:mmu-mir-6350-Gm22419 4:intergenic:Ighg1-Ighg3 4:intergenic:Ccdc175-Rtn1 4:intron:Wnt4 4:intron:Fam72a 4:intergenic:Igha-Ighe 4:intergenic:Gm25609-Gm23534 4:intergenic:Ppp1r2-ps2-Nup210 4:intergenic:Wfikkn2-Gm21885 4:intron:Ica1 4:intron:Masp2 3:exon:Gm13482 4:intron:Cntn5 2:intergenic:Gm26131-Klhl13 4:intergenic:Fut10-Gm23539 4:exon:Piwil1 4:intergenic:Gm25545-Gucd1 4:intergenic:Ptprm-Gm22041 4:intergenic:Gpr133-Sfswap 3:intron:Pard3b 4:intergenic:Gm26603-Fmnl2 2:intron:D11Wsu47e 4:intergenic:n-R5s172-Gm26486 3:intergenic:Gm13811-Gm13813 4:intergenic:Gm23291-Kcnk13 4:intron:Psd2 4:intron:C8b 3:intergenic:Gm8325-P2ry1 4:intergenic:Cacul1/Gm25238-Nanos1 4:intergenic:Ntf3-Kcna5 4:intergenic:Ahcy-2310005A03Rik 4:intergenic:Gm17337-4930519A11Rik 4:intergenic:Zswim6-Gm22118 4:intron:Arhgef7 4:intergenic:4933427E11Rik/Jrk-Psca 4:intergenic:Gm8429-Rpl21-ps4 4:intergenic:Gm22024-Gm26721 4:intergenic:Chst15-Oat 4:intron:9130204K15Rik 3:intron:Hydin 4:intron:Ptprn2 4:intergenic:Gm22347-Gm23764 4:intergenic:Gm24462-C230034O21Rik 4:intron:Bpifb1 4:intron:Fam195a 4:intergenic:Arrdc4-Gm23795 4:intergenic:Gm10775-Zfp369 4:intergenic:Gm22618-Gm22976 4:intergenic:Gm13003-Gm25399 4:intergenic:D630014O11Rik-Galnt14 4:intron:Sectm1b 4:intergenic:Gm15766-Gm6288 4:intergenic:Adam1b-Tmem116 4:intergenic:Mc4r-Gnal 4:intron:Acad11 Off-target primers show all... show less...
164 / fw	`GGCCTAACTTGAGCTGGGCG TGG` `.................... ...` Cloning / PCR primers	76	85	55	36	60	83	0 - 0 - 0 - 9 - 93 0 - 0 - 0 - 1 - 5 102 off-targets	3:intergenic:Gm11867-Gm11868 4:exon:Gm3848 4:intergenic:Gm22025-Mid2 4:intron:Clvs1 4:intergenic:Dagla-Syt7/Gm26859 4:intron:Nol4 4:intron:Acox1 4:intron:Hmcn1 4:intergenic:RP23-433F5.5-RP23-433F5.6 4:intron:Ubxn11 4:intergenic:Atp2b4-Optc 4:intergenic:Gm22183-mmu-mir-378d 4:intron:Gc 4:intergenic:Gm9103-Rps4x 4:intergenic:Gm23178-Gm6878 4:intron:Ankrd28 4:intron:Ube3c 4:intron:Rnf220 4:intergenic:Col22a1-Gm23217 4:intergenic:Gm26182-Gm14929 4:intergenic:Rnf182-Cd83 4:intergenic:Gm14534-5730405O15Rik 4:intron:Mef2a 4:intergenic:Add2-Tgfa 4:intergenic:Klf14-Mir29a 3:intron:Steap3 4:exon:Bai1 4:intron:Ppp2r5c 4:intergenic:Etv6-Bcl2l14 4:intergenic:Gm13811-Gm13813 3:exon:Zfp618 4:intergenic:Clic5-Runx2 4:intergenic:4933402N22Rik-Sema3d 4:intron:Pard3b 4:intergenic:Gm8935-Gm2464 4:intergenic:Gm26704-Baalc 4:intron:Glrx2 4:intergenic:BC048507-Mtrr 4:intron:Rtn4rl1 4:intergenic:Trmt10c-Gm24047 4:intergenic:Fer-Pja2 4:intergenic:Gm9621-Gm24200 4:intron:Trp53bp1 4:exon:Ado 4:intergenic:Pcdh11x-H2afb3 4:intron:Parp9 4:intron:E230015J15Rik 3:intergenic:Gm25718-4930594M17Rik 4:intergenic:Gm12152-Pwwp2a 4:exon:Stra8 3:intergenic:Gm11344-Cmah 4:intergenic:Mfsd1-Iqcj 4:intron:Acvr1b 4:intron:Dcc 4:intergenic:Zfp422-rs1-Zfp955b 4:intron:Zfp955a 4:intron:Acly 4:intergenic:Gm23036-Gm22034 3:intergenic:2310042E22Rik-Ephb3 4:intron:Immp2l 4:intron:Itm2c 4:intron:Ppp1r12b 3:intron:Mia3 4:intron:4933436C20Rik 4:intergenic:Gm13555-Slc4a10 4:intron:Exph5 3:intergenic:Gm22773-Bmp7 4:intron:Map3k5 4:intergenic:Gm8988-Gm23095 4:intron:Tex2 4:intron:Klk1b1 4:intron:Dtl 4:intergenic:Tcf23-Slc5a6 4:intron:Megf6 4:intergenic:Gm266-Eif5 4:intron:Tpk1 4:intron:Dcp1b 4:intron:2510039O18Rik 4:intergenic:Pde7b-4930403O15Rik 4:intergenic:Gm23728-Foxb1/B230323A14Rik 4:intergenic:Rxfp4-2810403A07Rik 4:exon:Chtop 4:exon:Mettl13 4:intergenic:Gm25297-Dlk1 4:intergenic:Kcne1-Rcan1 4:intergenic:Pabpc4-Bmp8a 4:intergenic:Oxgr1-Mbnl2 4:intron:Krt39 3:intergenic:Gpatch1-Rhpn2 4:intron:Slc41a1 4:intergenic:Gpr183-Ubac2 4:intergenic:Gm25216-Pyroxd2 4:exon:Rbak 4:exon:Rnf40 4:intergenic:4930479D17Rik-Stk38l 4:exon:Olfr1010 4:intergenic:Gm24710-Gm24889 4:exon:Kcnmb1 4:intron:Prrg1 4:exon:Bri3bp 4:intergenic:Gm15177-Gm22522 4:intron:Gpr157 Off-target primers show all... show less...
354 / rev	`GATATATAGAGACACTTAAC AGG` `.................... ...` Enzymes: Tru1I Cloning / PCR primers	75	88	44	37	71	82	0 - 0 - 2 - 12 - 145 0 - 0 - 1 - 2 - 1 159 off-targets	4:intergenic:Gm14422-Prkcb 3:intergenic:Gm26069-Gm25811 4:intron:Gm16630 4:intergenic:Gm14513-Gm26314 4:intergenic:Fat2-Gm12234 4:intron:Actr3b 4:intron:Dmd 4:intergenic:Acap2-Ppp1r2 4:intron:Arglu1 4:intergenic:Camk2a-Slc6a7 4:intergenic:Rpl13-ps3-1700129C05Rik 4:intergenic:Kcnc2-Atxn7l3b/Gm26596 4:intron:Pde4d 4:intron:Atg5 4:intron:Nrxn1 4:intergenic:Gm21905-Gm5108 4:intron:Bri3bp 3:intron:Vldlr 4:intergenic:Gm12883-Gm12886 4:intron:Slc35f3 4:intron:Caln1 4:intergenic:Crispld1-Crisp4 4:intergenic:Rph3al-1700016K19Rik 4:intergenic:Gm11675-Gm11676 4:intron:Stk33 4:intron:Osmr 3:intron:Nrbp1 4:intergenic:Gm4918-Hmgb1-ps2 4:intergenic:Snrpn/Snurf-Snrpn 4:exon:Far1 4:intron:Spag6 3:exon:Vav2 4:intergenic:Unc45b-1700003F17Rik/Unc45b 4:intron:Piezo2 4:intergenic:Gm6373-Gucy2f 4:intergenic:Sh2d2a-Prcc 4:intergenic:Gm15501-Gm5899 4:intergenic:Gm5124/Gm26593-Agtr2 4:intron:Mei4 4:intergenic:Gm14656-Gm16411 4:intron:Sorcs1 4:intron:Mtfr2 4:intergenic:Dlg2-Gm23928 4:intergenic:Gm14521-Gm14635 4:intergenic:Ccdc50-Fgf12 4:intron:Catsper4 4:intron:Ppa2 4:intron:Svil 4:intron:Pdk2 4:intron:Grik2 4:intergenic:Igsf11-Gm24795 4:intergenic:Gm22495-Dtna 4:intergenic:Ppp4r2-Pdzrn3 4:intergenic:Papss1-Gm26103 4:intron:Epb4.1l4a 4:intron:Kif5a 4:intergenic:4930412D23Rik-Gm25061 4:intergenic:Rpl31-ps25-AC135509.1 4:intergenic:Gm22782-Rpl10l 4:intron:Slc10a7 4:intergenic:Gm25041-Gm25853 4:intergenic:Gm23186-Arid1b 4:intron:Myom2 4:intergenic:Gm26844-Gm4812 4:intergenic:D17Wsu92e-Rps2-ps9 4:intergenic:Pcdh15-Gm15398/Gm5778 4:intergenic:D3Ertd751e-Gm25714 4:intron:Acad12 4:intergenic:Zfy2-Gm22119 4:intron:Snrpn 4:intergenic:n-R5s209-Gm7846 4:intron:Zbtb20 3:intergenic:Gm5611-Chordc1 4:intergenic:Lhfp-Nhlrc3/Gm16206 4:intergenic:Gm5839-Gm25440 3:intergenic:Cmas-Gm766 4:intergenic:Bcl2a1a-Gm24463 4:intergenic:Susd5-4921528I07Rik 4:intergenic:Gm26567-Gm2382 4:intron:Gm10634 4:intron:Gm26611 4:intergenic:Bcl2a1c-4930520O04Rik 4:intergenic:Susd5-4921528I07Rik 4:intergenic:Trim43b-Gm26567 4:intergenic:Tcof1-Arsi 4:intergenic:Tmprss2-Ripk4 4:intergenic:Gm10220-Gm7361 2:intergenic:Gm26674/Sash1-Gm9930 4:intergenic:Rbbp8-Gm6277 4:intergenic:Prex2-A830018L16Rik 4:intron:Grm1 4:intergenic:Gm23452-Gm23900 4:intergenic:Egln3-Gm22220 4:intron:Rftn1 4:intergenic:Olfr655-Olfr656 4:intron:Camta1 4:intergenic:AC133095.1-Gap 4:intergenic:Gm6468-Gm3259 4:intergenic:AC163611.1-C87414 4:intergenic:Gm6502-AA792892 4:intergenic:Gm16429-BC061212 4:intergenic:Gm3286-Gm7978 4:intron:Dennd3 4:intergenic:Slc17a6-Gm7336 3:intergenic:D7Ertd443e-Dock1 4:intergenic:Syndig1l-Npc2 4:intron:Slc24a2 4:intergenic:Cabin1-Ddt/Gm20441 4:intron:Kcnd2 4:exon:Brd8 4:intergenic:B4galt6-Trappc8 3:intergenic:Gm14919-Gm14920 4:intergenic:Gm20713/Gpc5-Gm20713 4:intergenic:Olfr205-Olfr206 3:intergenic:Gm25076-Gm26455 4:intron:Spata5 4:intergenic:Col22a1-Gm23217 4:intron:Ophn1 3:intron:Cntnap5b 4:intergenic:Gm25264-Gm25959 4:intergenic:Gm13490-Gm13492 4:intron:Fam189a2 4:intergenic:Nus1-Zfa-ps 4:intergenic:Gm24880-Gm10172 2:intergenic:St3gal1-Zfat 3:intergenic:Gm6781-Ube2e2 4:intergenic:A930011G23Rik-Gm26252 4:intergenic:Gm25051-Gm1818 4:intron:Zfp459 3:intergenic:Plk2-Actbl2 4:intron:Kcnd3 4:intron:Pcdh15 4:intron:Car10 4:intron:Spag16 4:intron:Myo3a 4:intergenic:Lrriq3-Gm23038 4:intergenic:Trbv26-Trbv29 4:intergenic:Opn5-Gpr115 4:intergenic:4931417E11Rik-Gm26179 4:intron:Cfh 4:intergenic:Gm23041-Nav3 4:intron:Ankrd44 4:intron:Fam47e 4:intergenic:Gm26181-Triml1 4:intergenic:Gm9843-Usp25 4:intergenic:Baz1a-2700097O09Rik 4:intron:Ccdc171 4:intergenic:Gm24246-Pik3c3 4:intergenic:Celf2-Gm24340 4:intergenic:Gm11418-5530401A14Rik 4:intron:Clip4 4:intergenic:Eya4-Rps12 4:intergenic:Gm10051-Gm15627 4:intergenic:Gxylt2-Gm15576 4:intergenic:Gm24393-RP23-452E7.2 4:intron:Thsd7b 4:intergenic:Gm15165-Gm6987 4:intergenic:3830408C21Rik-Apoo-ps 4:intron:Msn Off-target primers show all... show less...
124 / fw	`TTAAACTGGGATTAGACTTC AGG` `.................... ...` Inefficient Cloning / PCR primers	74	87	31	28	61	66	0 - 0 - 0 - 18 - 133 0 - 0 - 0 - 0 - 5 151 off-targets	4:intergenic:Gm5046-Gm23907 3:intergenic:Lbp-9430008C03Rik 4:intergenic:Vmn2r87-Gm23793 4:exon:A830082K12Rik 3:intergenic:Rtp4-Gm6640 4:intergenic:Gm24683-Gm14904 4:intron:Lrrc7 4:intergenic:Grik4-Gm23526 3:intergenic:Gm26901-Sntg1 4:intron:Gramd1b 4:intergenic:Olfr1267-ps1-Olfr1268-ps1 3:intron:Grik2 4:intron:Hivep1 4:intergenic:Gm25660-Slc27a6 4:intergenic:Gm23892-Gm25382 4:intergenic:Serp2-Lacc1 3:intergenic:Fam196b-Dock2 4:intergenic:Gm6317-Gm25383 4:intergenic:n-R5s198-Gm13652 3:intergenic:Gm5742-Gm22223 4:exon:Kcnn3 4:intron:Wdsub1 3:intron:Faf1 4:intergenic:Gm13531-Gm13532 4:intergenic:Gm23317-Lrrc4c 4:intron:Ash1l 4:intergenic:B230110C06Rik-Oxsm 4:intergenic:Gm22976-Ppargc1a 4:intron:Pard3 4:intron:Cntn6 4:intergenic:Gm5513-Gm24319 4:intergenic:Fzd5-Plekhm3 3:intergenic:Lbr-Gm5533 3:intron:Kalrn 4:intron:Src 4:intergenic:Ins1-Xpnpep1 4:intergenic:Gm23905-Zfp36l2 4:intergenic:Gm11224-Gm11225 4:intergenic:Gm5611-Chordc1 4:exon:Slc2a2 3:intron:Slc6a17 3:intergenic:Sema3c-Cd36 4:intergenic:Mtdh-Laptm4b 3:intron:Psma3 4:intergenic:Gm24692-Sptssa 4:intron:Exoc6b 4:intergenic:Mcpt9-Mcpt2 4:intron:Slc35a1 4:intergenic:Gm26468-Rpsa-ps10 4:intergenic:Hsp90aa1-Wdr20a 4:intergenic:Lgr5-Tspan8 4:intron:Acss3 4:intergenic:Gm24526-Gm25064 4:intron:Rad51b 4:intron:Anapc1 4:intergenic:Dnah5-Mir3964 4:intergenic:Olfr730-Olfr731 4:intron:Gm16630 4:exon:4932435O22Rik 4:intergenic:Gm16081-Daf2 4:intergenic:2900079G21Rik-AC113095.1 4:intergenic:Snx18-Hspb3 4:intron:Trpm1 4:intron:Vmn2r33 4:intron:Vmn2r43 4:intron:Vmn2r38 4:intron:Gm3665 4:intron:Vmn2r-ps40 4:intron:Gm3563 4:intron:Gm3616 4:intron:Vmn2r44 4:intron:Vmn2r-ps51 4:intron:Vmn2r-ps49 4:intron:Gm3683 4:intron:Vmn2r35 4:intron:Vmn2r31 4:intron:Vmn2r-ps43 4:intron:Vmn2r39 4:intron:Gm3584 4:intron:Vmn2r50 4:intron:Gm3623 4:intron:Tax1bp3 4:intergenic:2310005A03Rik-Itch 4:intergenic:Gm15074-Gm4996 4:intergenic:Irgm2-4930438A08Rik 4:intergenic:Ap2a1-Tsks 4:intron:Fam227a 4:intron:Spag9 4:intergenic:Gm25096-Gm25634 4:intergenic:Gm13221-Gm13219 4:intergenic:Cpa6-Gm15603 4:intron:Tbc1d10a 4:intergenic:Irx1-Rpl9-ps4 4:intergenic:Fam78b-Uck2 4:intergenic:Mb21d2-Hrasls 4:intergenic:Gm24839-Tmem132c 4:intron:Ephb2 4:intergenic:Etl4-Gm13335 4:intergenic:Gm25199-Syt6 4:intron:Astn1 4:intron:Gpr133 4:intergenic:Gm24648-Gm5499 4:intergenic:Gm22482-Rnls 4:intergenic:Gm4409/Lrrtm4-Lrrtm4 4:intergenic:Gm25051-Gm1818 4:intron:Arhgap24 4:intron:Ube2n 4:intergenic:Gm8399/Gm25241-9330111N05Rik 4:intergenic:Cmpk2-Gm9866 4:intergenic:Olfr1505-Gm26993 4:intergenic:Gm10797-Zfp365 4:intron:Hibch 4:intergenic:Gm23799-Gm25917 4:intergenic:Gm15053/HOTTIP_4-5730457N03Rik 4:intron:Cnnm4 4:intergenic:Pcdh9-Gm23324 4:intergenic:Klra5-Gm15854 4:intron:Faah 4:intergenic:Pou6f2-Vps41 4:intron:Col14a1 3:intron:Rab22a 4:intergenic:E230019M04Rik-Gm15046 4:intron:Hs6st3 4:intron:Olfr1508 4:intergenic:Csmd1-Gm25665 4:intergenic:Foxp2-Mdfic 4:exon:Nyap2 4:intron:Slc25a53 4:intergenic:Krt19-Krt9 4:intergenic:Fam150a-St18 4:intron:Abcg5 4:intergenic:3110053B16Rik-Gm21989 4:intron:Slc35f1 4:intergenic:Gm25248-Gm26326 4:intergenic:Gm27039-Adcyap1 4:intergenic:Gm5530-mmu-mir-6344 4:intergenic:Neo1-Gm7589 3:exon:Camk2d 4:intergenic:Gm11659-Mapt 3:intergenic:Gm5383-Mir221 4:intron:Sugp2 3:intergenic:4930445N18Rik-Zfp516 3:intergenic:Rassf9-Alx1 3:intergenic:Olfr1457-Olfr1458 4:intergenic:Gm26235-Gm25392 4:intergenic:Gm14797-Gm649 4:intergenic:Rgs17-Gm10945 4:intergenic:Fjx1-Pamr1 4:intergenic:Spata31-Spata31d1c 4:intergenic:4930405D11Rik-Kif2b 4:intergenic:Gm14945-Gm4992 Off-target primers show all... show less...
396 / rev	`GTTTATTACCTGGTCTCTGA AGG` `.................... ...` Enzymes: Hpy188I, AcuI Cloning / PCR primers	72	86	47	32	65	84	0 - 0 - 2 - 13 - 132 0 - 0 - 0 - 3 - 8 147 off-targets	4:intergenic:Gm22039-Prdm2 4:intergenic:Cdh6-Gm9630 4:intron:Cntnap2 4:intergenic:Atp2b1-Poc1b 4:intergenic:Prom2-Gm14229 4:intergenic:Gm13468-Gm13481 4:intergenic:A130030D18Rik-Gm13675 4:intergenic:Gm23838-Gm25233 4:intergenic:Gm23438-Gm22053 4:intergenic:Gm23072-Gm13469 4:intergenic:Cacna1c-Gm25905 4:intron:4930523C07Rik 4:intron:Dmgdh 4:intergenic:Gm11822-1110037F02Rik 4:intergenic:Cntnap4-Gm25766 4:intron:Sptlc3 4:intergenic:Frem2-Gm24851 4:intergenic:Gm24633-Gm25568 4:intron:Ubac2 4:intergenic:Gm14981-Gm26406 4:intron:Gpr144-ps 4:intergenic:Gm14682-Gm14690 4:intron:Eftud1 4:intergenic:Gpr139-Gm23321 4:exon:A230028O05Rik 3:intergenic:Gm26756-Shisa3 4:intron:Galnt1 4:intron:Dlgap2 3:intron:P2rx7 4:intron:Adam28 4:intergenic:Mir3072/Mirg-Dio3os 4:exon:Lctl 4:intergenic:Zfp438-Gm10125 4:intron:Arhgef4 4:intergenic:Macrod2-Gm25583 4:intergenic:Gm22602-Anks1b 4:intergenic:Pglyrp2-Cyp4f39 4:intergenic:Nxpe3-Cep97 4:intergenic:Gm25656-Cntn6 4:intergenic:Sorl1-Sc5d 4:intergenic:Arg1-Gm22566 4:intergenic:n-R5s194-Gm23937 4:intron:Zp3r 4:intron:Ccser1 4:intergenic:Atp11c-Mir505 4:intergenic:Megf9-Gm11227 4:intergenic:Adamts5-Gm25715 4:intergenic:Olfr1480-Olfr1484 4:intergenic:Gm14074-Plcb4 4:intron:Fam53b 4:intergenic:3830417A13Rik-Slitrk4 4:intergenic:AC124326.1-Mir802 4:intergenic:Mst1r-Actl11 4:intergenic:Gm24326-Gm25923 4:intergenic:Gm25099-Gm23143 4:intergenic:9530052E02Rik-Gm15418 4:intergenic:4930586N03Rik-Abt1 4:intron:Pkd1l1 4:exon:A630075F10Rik 4:intergenic:Satb1-RP24-465O15.1 4:intergenic:Serpinb8-Gm15389 4:intergenic:Gm6245-Gcnt2 4:intergenic:AC161214.1-Gm11033 4:intergenic:Gm25272-Itgbl1 4:intergenic:6330403A02Rik-Parp1 2:intergenic:Gm10709-Tmem123 3:intron:Gria2 4:intron:Gabrg3 3:intergenic:Pxdn-Tpo 3:intergenic:Gm6404-Isl1 4:intergenic:Gm9897-Fzd1 4:intergenic:PVT1_3-Ndufa4 4:intergenic:Nek7-Lhx9 4:intergenic:Gm21614-Gm23249 4:intron:Arhgap18 4:intergenic:AC158354.1-Gm4804 4:intergenic:Arsj-Gm17225 4:intergenic:Tnp1-Pinc 4:intron:Slc7a8 4:intron:Ccdc83 4:intron:Fam168b 4:intergenic:Adcy5-Gm25179 4:intergenic:Gm26228-Gdf10 4:intergenic:Acta2-Fas 4:intergenic:Nmbr-Gm23892 4:intron:Rrm2b 4:intergenic:Clec14a-Gm17529 3:intron:Brox 4:intergenic:Gm6634-Gm8604 4:intron:Ano10 2:intron:Arhgap17 4:intron:Zfp521 4:intergenic:Gm25460-Gm5263 4:intergenic:Olfr1408-Olfr1407-ps1 3:intergenic:Rps24-4930542C16Rik 4:intergenic:Gm15292-Defa-ps8 4:intergenic:Gm20713-Gpc5 4:intron:Dlgap2 4:intergenic:Agpat9-Gm20484 4:intergenic:Rfwd2-Gm10530 4:intergenic:n-R5s50-Gm23242 4:intergenic:Gm5611-Chordc1 3:intron:Trappc13 4:intergenic:Ero1l-Psmc6 4:exon:1700008P02Rik 3:intergenic:Gm9800-Fhit 4:intergenic:Gm25336-Zfp648 4:intron:Synpo2 4:intergenic:Gm14505-Gm9435 4:intergenic:Gm16565/Cd247-Gm23402 3:intergenic:Drd1a-Sfxn1 4:exon:Scyl2 4:intergenic:Grik1-Cldn17 3:intergenic:Emp2-Tekt5 4:intron:Cd274 4:intergenic:AC161571.1-Gm25956 4:intergenic:Tacr3-Gm24048 3:exon:4930547C10Rik 4:intron:Cmss1 4:intergenic:Iqcf4-Iqcf6 4:intergenic:Gm12347-Krt13 4:intergenic:Mtpap-9430020K01Rik 3:intergenic:Gm25369-Akap12 4:intergenic:4930555K19Rik-Oxr1 4:exon:Ccp110 4:intergenic:4930583I09Rik-Gm25344 4:intron:Spns3 4:intergenic:Ppp1r18/5530401N12Rik/CR974451.1-Ppp1r18 4:intergenic:Rps16-ps3-3930402G23Rik 4:intron:Sh3rf2 4:intron:Ptgr2 4:intergenic:Zfp87/Gm26965-Zfp748 4:intergenic:Gm11956-Osm 4:intron:Dmd 4:exon:Ctsh 4:intergenic:Gm11879-Gm11883 4:intergenic:Gm11902-Gm11901 4:intergenic:Vmn2r100-Vmn2r101 4:intergenic:Gm10108-Sema3a 4:intergenic:Gm9931-Gm22966 4:intergenic:Ly86-Rreb1 4:intergenic:Gm23041-Nav3 4:intergenic:Pak4-Pak4/Samd4b 4:exon:Ybx2 4:intergenic:4930401O10Rik-Gm23226 4:intergenic:B3galtl-Rxfp2 4:exon:Cdh12 Off-target primers show all... show less...
426 / fw	`CCAGGTAATAAACTAAAGAC TGG` `.................... ...` Enzymes: BmuI, Bse1I Cloning / PCR primers	72	82	50	20	63	81	0 - 0 - 5 - 13 - 398 0 - 0 - 1 - 1 - 0 416 off-targets	3:intron:Actn2 2:intergenic:Gm24510-Nrxn1 3:intron:Msn 4:intergenic:Rock1-Greb1l 2:intergenic:Cyp3a41b-Cyp3a41a 3:intron:Nfia 3:intergenic:Cyp3a16-Cyp3a41b 4:intron:Ntm 4:intron:Arhgef10l 4:intron:Rasgrp3 4:intergenic:Tapt1-Ldb2 4:intergenic:Gm24275-Gm12291 4:intergenic:Gm14304-Smox 4:intergenic:Arhgef28-Utp15 4:intergenic:Gm12129-Gm12130 4:intergenic:Olfr1174-ps-Olfr1175-ps 4:intron:Lingo2 4:intergenic:4930548G14Rik-Gm23665 4:intron:Phex 4:intron:4933427D06Rik 4:intergenic:Gm12636-Gm12632 4:intergenic:Gm10275-Cenpw 4:intergenic:Defb11-Defb15 4:intron:Plk1s1 4:intergenic:Gm25485-Gm12460 3:intron:Nckap5 4:intergenic:5033430I15Rik-Gm10113 4:intron:Sorcs3 4:intron:Sycp2 4:intron:Dgkk 4:intergenic:Gm14859-Cited1 4:intron:Gm26578 4:intergenic:Gm26928-Kcnn2 2:intergenic:Extl2-Gm26344 4:intergenic:Gm14982-Gm26209 4:intergenic:1700049L16Rik-Gm9923 4:intergenic:Gm11628-Gpatch8 3:intergenic:Hdac4-Ndufa10 4:intergenic:Gm9801-Ndn 4:intron:Itpr2 4:intergenic:Pcdh17-Gm23926 4:intergenic:Speer4e-Gm10354 4:intron:Speer7-ps1 4:intergenic:Gm17019-Gm21847 4:intergenic:Gm10354-Gm17019 4:intergenic:Gm23440-Rapgef2 4:intergenic:Clstn2-4921534H16Rik 3:intergenic:Cyp3a41a-Cyp3a44 4:intron:Cdkal1 4:intergenic:4631405K08Rik-Plxna2 4:intron:Plac1 4:intergenic:4930455H04Rik-1700061I17Rik 4:intergenic:Htr1a-Dph3b-ps 3:intergenic:Cacna1g-Spata20 4:intron:Phkg1 4:intergenic:Gm6462-Gm25586 4:intergenic:Evi5-Rpl5 3:intron:Dync2h1 4:intron:Nrcam 4:intergenic:Gm4725-Gm9498 4:intron:Rcbtb2 4:intergenic:Cypt1-Csnk2a1-ps 4:intron:Wbscr17 4:intergenic:Prdm1-Gm22087 4:intron:Fars2 4:intergenic:Fpr-rs4-Vmn2r124 4:intergenic:Gm15160-Gm15155 4:intron:Gpr110 3:intergenic:Gm11350-Gm11351 4:intergenic:Gm14020-Frmd5 4:intergenic:Gm5974-Gm23240 4:intergenic:Gm11810-Rab2a 4:intergenic:Snrpd3-Fam211b 4:intergenic:Gbp2-Gbp2/mmu-mir-6381 4:intergenic:Mycn-Gm24208 4:intergenic:Gm5916-RP23-319C8.4 4:intergenic:Nek10-Lrrc3b 4:intergenic:Gm10259-Nlgn1 4:intergenic:Gm22891-Gm24774 4:intergenic:Gm7134-Gm9225 4:intergenic:BC051070-2010016I18Rik/Dennd2d 4:intergenic:Gm24691-Grm8 4:intron:Negr1 4:intergenic:Gm14555-Rhox12 4:intron:Msh3 4:intergenic:RP23-16D16.1-Tcf12 4:intergenic:Bms1-Zfp248/Gm25953 4:intergenic:Gm16039-Gm24059 4:intron:R74862 4:intron:Rorb 4:intergenic:0610039K10Rik/Serinc3-Pkig 4:intergenic:Gm11703-Gm23699 4:intergenic:Kcns3-Msgn1 4:intergenic:Crry-ps-AC161214.1 4:intergenic:Eepd1-Gm25861 4:intergenic:Gm7545-Gm10443 4:intergenic:Brap-Atxn2 4:intergenic:Gm20822-Gm21809 4:intergenic:Gm20828-Gm20812 4:intergenic:Gm10247-Grin2a 4:intergenic:Gm14970-4930412D23Rik 4:intergenic:Rbfox1-Gm23476 4:intron:Lrp1b 4:intergenic:4930578C19Rik-Gm6923 4:intergenic:Plod2-Gm9621 4:intergenic:Slc2a2-1700112D23Rik 4:intergenic:Ccser1-Gm22212 4:intron:Tnfsf15 4:intron:Sltm 4:intergenic:Gm22331-Gm24937 4:intergenic:Cenpw-Gm22623 4:intergenic:Magee1-Gm6292 4:intron:Prkce 4:intergenic:Syndig1-Gm14140 4:intergenic:Stox2-4930448N21Rik/Gm10083 4:intron:Hecw2 4:intergenic:Arap3-Pcdh1 4:exon:Zfp697 4:intergenic:Igkv15-103-Igkv14-100 4:intergenic:Mb21d2-Hrasls 4:intergenic:Sorcs2-mmu-mir-7689 4:exon:Fxyd4 4:intron:Vmn2r93 4:intron:Slc39a12 4:intergenic:Gm15246/BC022960-Gm15245 4:intergenic:Asph/Clvs1-Asph 4:intergenic:Olfr344-Olfr345 4:intergenic:Eif3s6-ps2-Gm25418 4:intergenic:Cyp3a41a-Cyp3a44 4:intron:Col6a6 4:intergenic:Acaa2-Gm24559 4:intergenic:Unc5d-4933433F19Rik 4:intergenic:Gm24295-Mef2c 4:intergenic:Gm15531-Mitf 4:intergenic:Gm13850-Plxna4 4:intergenic:Olfr231-Olfr424 4:intergenic:Pacsin2-Ttll1 4:intergenic:Gm5121-Ppcdc 4:intron:Sema5a 4:intergenic:Gm25041-Gm25853 4:intergenic:Gm9192-Gm23735 4:exon:Htr2c 4:intergenic:Snx16-Gm22795 4:intergenic:Gm21874-Gm20821 4:intergenic:Gm21572-Gm21753 4:intergenic:Gm21874-Gm20821 4:intergenic:Gm21902-Gm21285 4:intergenic:Gm20865-Gm21065 4:intergenic:Gm21895-Gm20933 4:intergenic:Gm20826-Gm20825 4:intergenic:Gm20881-Gm20889 4:intergenic:Gm21247-Gm21256 4:intergenic:Gm21788-Gm20826 4:intergenic:Gm21810-Gm21916 4:intergenic:Gm20901-Gm21865 4:intergenic:Gm21858-Gm21340 4:intergenic:Gm21801-Gm21914 4:intergenic:Gm20828-Gm20812 4:intergenic:Gm21777-Gm21792 4:intergenic:Gm20822-Gm21809 4:intergenic:Gm21242-Gm21248 4:intergenic:Gm20935-Gm20937 4:intergenic:Gm21198-Gm20808 4:intergenic:Gm21729-Gm21774 4:intergenic:Gm21855-Gm20902 4:intergenic:Gm21163-Gm21180 4:intergenic:Gm21824-Gm21879 4:intergenic:Gm20837-Gm21860 4:intergenic:Gm20840-Gm21845 4:intergenic:Gm21650-Gm20867 4:intergenic:Gm21835-Gm21878 4:intergenic:Gm21775-Gm21900 4:intergenic:Gm20816-Gm20818 4:intergenic:Gm21821-Gm21842 4:intergenic:Gm21163-Gm21180 4:intergenic:Gm20837-Gm21860 4:intergenic:Gm21805-Gm21241 4:intergenic:Gm21076-Gm25359 4:intergenic:Gm20843-Gm20844 4:intergenic:Gm20931-Gm21733 4:intergenic:Gm21476-Gm21506 4:intergenic:Gm21914-Gm21852 4:intron:Nalcn 4:intergenic:Gm20736-Gm20936 4:intergenic:Gm21862-Gm23863 4:intergenic:Gm20923-Gm21249 4:intergenic:Gm21095-Gm21380 4:intergenic:Gm21170-Gm21751 4:intergenic:Gm21588-Gm21599 4:intergenic:Gm21679-Gm21529 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm21734-Gm24828 4:intergenic:Gm20865-Gm21065 4:intergenic:Gm21745-Gm21184 4:intergenic:Gm21767-Gm20822 4:intergenic:Gm20810-Gm21853 4:intergenic:Gm21562-Gm21572 4:intergenic:Gm21723-Gm21821 4:intergenic:Gm20816-Gm20818 4:intergenic:Gm21802-Gm25729 4:intergenic:Gm21892-Gm23770 4:intergenic:Gm21529-Gm21539 4:intergenic:Gm5403-Mdm4-ps 4:intergenic:Gm21766-Gm20800 4:intergenic:Gm20901-Gm21865 4:intergenic:Gm21767-Gm20822 4:intergenic:Gm20884-Gm21387 4:intergenic:Gm20825-Gm20815 4:intergenic:Gm21745-Gm21184 4:intergenic:Gm21745-Gm21184 4:intergenic:Gm21760-Gm21257 4:intergenic:Gm21918-Gm21832 4:intergenic:Gm20914-Gm21778 4:intergenic:Gm21779-Gm21247 4:intergenic:Gm21808-Gm20866 4:intergenic:Gm20913-Gm21868 4:intergenic:Gm21861-Gm21758 4:intergenic:Gm21758-Gm21843 4:intergenic:Gm21617-Gm21634 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm21792-Gm21287 4:intergenic:Gm21722-Gm21282 4:intergenic:Gm21333-Gm21344 4:intergenic:Gm21841-Gm20842 4:intergenic:Gm21861-Gm21758 4:intergenic:Gm21923-Gm20880 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm20892-Gm21771 4:intergenic:Gm20932-Gm21524 4:intergenic:Gm21633-Gm21745 4:intergenic:Gm21247-Gm21256 4:intergenic:Gm21599-Gm21810 4:intergenic:Gm20810-Gm21853 4:intergenic:Gm21117-Gm20884 4:intergenic:Gm21805-Gm21241 4:intergenic:Gm21111-Gm21875 4:intergenic:Gm21198-Gm20808 4:intergenic:Gm21776-Gm21155 4:intergenic:Gm20794-Gm21918 4:intergenic:Gm20916-Gm21823 4:intergenic:Gm20902-Gm21715 4:intergenic:Gm21626-Gm21633 4:intergenic:Gm21722-Gm21282 4:intergenic:Gm21804-Gm20810 4:intergenic:Gm21171-Gm20924 4:intergenic:Gm21518-Gm20823 4:intergenic:Gm21913-Gm22832 4:intergenic:Gm21539-Gm21908 4:intergenic:Gm20907-Gm20921 4:intergenic:Gm20822-Gm21809 4:intergenic:Gm21822-Gm21763 4:intergenic:Gm8521-Gm20918 4:intergenic:Gm21724-Gm21405 4:intergenic:Gm20810-Gm21853 4:intergenic:Gm20799-Gm21795 4:intergenic:Gm21861-Gm21758 4:intergenic:Gm20830-Gm21244 4:intergenic:Gm21720-Gm21838 4:intergenic:Gm21654-Gm20882 4:intergenic:Gm20815-Gm21292 4:intergenic:Gm21889-Gm20852 4:intergenic:Gm21815-Gm20913 4:intergenic:Gm21634-Gm21654 4:intergenic:Gm21766-Gm20800 4:intergenic:Gm21739-Gm21856 4:intergenic:Gm21151-Gm21160 4:intergenic:Ssty2-Gm21114 4:intergenic:Gm20806-Gm20919 4:intergenic:Gm21524-Gm21672 4:intergenic:Gm21412-Gm21773 4:intergenic:Gm16630-Gm22122 4:intergenic:Gm21679-Gm21529 4:intergenic:Gm21209-Gm21316 4:intergenic:Gm21906-Gm21920 4:intergenic:Gm21843-1700040F15Rik 4:intergenic:Gm21679-Gm21529 4:intergenic:Gm21170-Gm21751 4:intergenic:Gm20828-Gm20812 4:intergenic:Gm21257-Gm20791 4:intergenic:Gm21805-Gm21241 4:intergenic:Gm21443-Gm21755 4:intergenic:Gm20822-Gm21809 4:intergenic:Gm21919-Gm21790 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm21506-Gm20891 4:intergenic:Gm20738-Gm21924 4:intergenic:Gm20813-Gm21922 4:intergenic:Gm21908-Gm21780 4:intergenic:Gm20825-Gm20815 4:intergenic:Gm20809-Gm21921 4:intergenic:AC201846.1-Gm20910 4:intergenic:Gm21779-Gm21247 4:intergenic:Gm21758-Gm21843 4:intergenic:Gm20794-Gm21918 4:intergenic:Gm21943-Gm21724 4:intergenic:Gm20798-Gm20896 4:intergenic:Gm20826-Gm20825 4:intergenic:Gm20798-Gm20896 4:intergenic:Gm21822-Gm21763 4:intergenic:Gm20898-Gm21799 4:intergenic:Gm21163-Gm21180 4:intergenic:Gm21735-Gm21732 4:intergenic:Gm21258-Gm21268 4:intergenic:Gm21661-Gm21907 4:intergenic:Gm20888-Gm20886 4:intergenic:AC201846.1-Gm20910 4:intergenic:Gm8521-Gm20918 4:intergenic:Gm21573-Gm20803 4:intergenic:Gm21787-Gm20901 4:intergenic:Gm21247-Gm21256 4:intergenic:Gm21160-Gm20932 4:intergenic:Gm21753-Gm21723 4:intergenic:Gm21803-Gm20837 4:intergenic:Gm21757-Gm20846 4:intergenic:Gm20901-Gm21865 4:intergenic:Gm20895-Gm20892 4:intergenic:Gm21894-Gm23052 4:intergenic:Gm21825-Gm21333 4:intergenic:Gm21813-Gm21777 4:intergenic:Gm20828-Gm20812 4:intergenic:Gm21244-Gm21788 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm20873-Gm21719 4:intergenic:Gm21901-Gm21864 4:intergenic:Gm21749-Gm21302 4:intergenic:Gm21427-Gm21890 4:intergenic:Gm21396-Gm21245 4:intergenic:Gm21724-Gm21405 4:intergenic:Gm21778-Gm21746 4:intergenic:Gm20893-Gm21642 4:intergenic:Gm21065-Gm21871 4:intergenic:Gm21247-Gm21256 4:intergenic:Gm21751-Gm21271 4:intergenic:Gm21829-Gm20912 4:intergenic:Gm20853-Gm20855 4:intergenic:Gm21715-Gm21784 4:intergenic:Gm21679-Gm21529 4:intergenic:Gm21573-Gm20803 4:intergenic:Gm20873-Gm21719 4:intergenic:Gm21877-Gm21530 4:intergenic:Elovl4-Ttk 4:intron:Olfr140 2:intron:Gm872 3:intergenic:Gm7816-Gm2810 4:intergenic:Rragb-Gm15153 4:intron:Pgr 4:intergenic:Dcc-Gm25509 4:intergenic:Gm5611-Chordc1 4:intergenic:Cntn5-Gm25365 4:intergenic:Gm25061-Gm5403 4:intron:Frmd3 4:intron:Copg2 4:intergenic:Gm26139-Gm27034 3:intergenic:Gm23154-Gm12604 4:intron:Ppara 4:intergenic:Gm10000-Dicer1 4:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:Gm14904-Gm24624 4:intergenic:Gm7763-Gm5185 4:intron:Mpp7 4:intergenic:Vmn1r229-Vmn1r230 4:intergenic:Gm5185-Gm24921 4:intergenic:Gm26928-Kcnn2 4:intergenic:Gm22828-Gm23960 4:intergenic:Usp9y-Zfy2 4:exon:Adipor1 4:intergenic:Gm5334-Arrdc4 4:intergenic:Ythdf3-Gm26485 4:intergenic:Pgk1-rs7-Kcns3 4:intergenic:Gm23132-Nufip2 4:intergenic:Gm7745-Lrfn5 4:intron:Mthfd1l 4:intergenic:Gm6369-Armc1 4:intergenic:Gm14015-Gm14014 4:intergenic:Gm11077-Pde3a 4:intron:Rspo1 4:intergenic:Gm25550-Cdh9 4:intron:Dcdc5 4:intergenic:Gm6317-Gm25383 4:intergenic:Gm17192-Gm24164 4:exon:Ttn 4:intergenic:Gm24790-Klf6 4:intergenic:Gm23965-Gm23537 4:intergenic:Gm8428-Gm16039 4:exon:Cldn15 4:intron:Rbfox2 4:intergenic:Gm14957-Gm14971 4:intron:Pianp 2:intergenic:Gm23881-Gm3534 3:intergenic:Sult2a2-Sult2a1 4:intron:B4galt6 4:intergenic:Fcho1-Zfp709 4:intergenic:Gm23013-Ppp1r3a 4:intergenic:Gm22225-Gm14986 4:exon:Lcp1 4:intergenic:Gm26686-Gm23056 4:intergenic:Mir466j-Gm20611 4:intron:Nrp1 4:intergenic:Gm13925-Gm20638 4:intergenic:Gm26171-Fam49a 4:intergenic:Cbln2-Gm5096 4:exon:Zfp704/C030034L19Rik 4:intron:Lmo7 4:intergenic:Gm26031-Gm9801 4:intergenic:Zfp423-Gm2716 4:intron:Galnt2 4:intergenic:AC161410.1-Haus8 4:intron:Thada 4:intergenic:Fut10-Gm23539 4:intergenic:Aff3-Lonrf2 4:intergenic:Gm7488-Tbl1xr1 4:intergenic:Gm23926-Gm25115 4:intergenic:Bcl2a1a-Gm24463 4:intergenic:Dysf-Cyp26b1 4:intron:Large 4:intergenic:Gm11908-Gm11909 Off-target primers show all... show less...
111 / fw	`CTTAAACTCAGAATTAAACT GGG` `.................... ...` Enzymes: BmuI, Bse1I Cloning / PCR primers	70	85	49	13	66	80	0 - 0 - 5 - 29 - 201 0 - 0 - 1 - 2 - 5 235 off-targets	4:intergenic:Gm22296-Gm23799 4:intron:Adam18 4:intergenic:Gm44-Gm14773 3:intron:Palld 4:intergenic:Flt1-Pomp 4:intron:Ift88 4:intron:Rims1 4:intergenic:Fam196b-Dock2 4:intergenic:Gm15826-Lancl1 3:intron:F13a1 4:intron:Grm3 4:intron:Gtdc1 4:intron:Ccdc171 4:intergenic:Robo2-Gm26307 4:intergenic:A1bg-Gm24696 4:intergenic:Gm14373-Gm14452 4:intron:Sgip1 4:intron:BC053393 4:intergenic:Usp11-Araf 4:intergenic:Gm5383-Mir221 4:intron:D10Bwg1379e 4:intron:Cadps 4:intergenic:Gm17213-Ppm1l 4:intergenic:Gm16330-Npy5r 4:intergenic:Gm24132-Gm25160 3:intergenic:Epas1-Tmem247 4:intron:Hmgxb4 4:intron:Zfp385c 4:intergenic:Gm25843-Tmeff2 4:intron:Ranbp9 3:intergenic:Gm7931-Slit2 4:intron:Dkk2 4:exon:Vmn2r112 4:intergenic:Gm12680-Sh3gl2 2:exon:AU019990 4:intron:Ptprj 3:intergenic:Gm22444-mmu-mir-6238 4:intron:Ano1 4:exon:Vmn2r114 4:intergenic:Gm22406-Spry2 4:intergenic:Gm12665-Gm24255 4:intergenic:Gm22845-Nt5c1b 3:intron:Sclt1 4:intergenic:mmu-let-7j-Gm6214 4:intergenic:Gm24738-Srd5a3 4:intergenic:Kcnma1-Gm6158 4:intron:Aff2 3:intron:Vps13b 4:intergenic:4933402N22Rik-Sema3d 4:intergenic:Gm5577-Rab7 4:intergenic:n-R5s58-Akap6 4:intron:Hpse2 4:intron:Enox1 4:intergenic:Vmn1r28-Vmn1r29 4:intergenic:Isoc1-Adamts19 4:intergenic:Gpm6a-Adam29 4:intergenic:Gm26662-Kirrel3 4:intron:Sufu 4:intergenic:Gm22157-Hivep1 4:intergenic:4930448K20Rik-Gm11834 4:intron:Samd12 4:intergenic:Gm15157-Sat1 4:intron:Slit2 3:intergenic:Gm22092-Gm12998 4:exon:Ino80b 4:intergenic:Gm23075-Gm22853 4:intergenic:Gm5356-Gm26331 4:intergenic:Fzd8-Gjd4 3:intron:Kcnh8 4:intergenic:RP24-376I11.1-RP23-402M18.1 4:intron:Macrod2 3:exon:Ap5z1 3:intron:Fra10ac1 4:intergenic:Pag1-Gm16337 4:intergenic:Gm15232-Gm15228 4:intergenic:Klhl15/mmu-mir-7673-Gm5400 4:intron:Slc44a1 3:intergenic:Olfr670-Olfr671 4:intron:Agtr1a 4:intergenic:Gm24104-Gm26058 4:intergenic:Gm23178-Gm6878 4:intergenic:Thnsl2-Fabp1 4:intron:Sfxn5 4:intergenic:Epha4-Gm26263 4:intergenic:Tpbg-Ube2cbp 3:intron:Col12a1 3:intergenic:Gm12913-Gm25836 4:intergenic:AW554918-Gm16558 4:intergenic:Gm22959-Daam1 4:intron:Gm12580 3:intergenic:D930016D06Rik-Gm8258 4:intergenic:Gm5453-Tnpo1 4:intergenic:Gm24731-Gm22076 3:intergenic:Gm24248-Rybp 4:intergenic:Gm13364-Gm6587 4:intergenic:Pcgf1-Lbx2 4:intergenic:Slitrk5-Gm22764 4:intergenic:Zfp940-Gm17199 4:intergenic:Gm9578-Gm22891 2:intergenic:AC100386.1-Gm8394 4:intron:Ccser1 4:intergenic:Gm23642-Lrfn2 4:intergenic:Gad1-ps-Csl 4:intron:Dennd1a 4:intron:Gpc6 3:intron:Bclaf1 4:intergenic:Gm3756-Arhgap32 4:intergenic:Chrm3-Gm26861 4:intron:Pabpc4 3:intron:Lrp1b 4:intergenic:Gm5839-Gm25440 4:intergenic:Osr2-Vps13b 4:intron:Acsl5 3:intron:Slc7a14 4:intergenic:Vim-St8sia6 4:intron:Mgat4a 4:intergenic:0610040F04Rik-Gm17055 4:intron:Cdk5rap1 4:intron:Eml1 4:intergenic:Tph1-Saal1 4:intron:Zfp568 3:intergenic:Arap3-Pcdh1 3:intron:4930452B06Rik 4:intergenic:Pgk1-rs7-Kcns3 2:intron:0610043K17Rik 4:intergenic:Sgcz-Gm16204 4:intergenic:Gm5973-Gm9915 4:intergenic:Cdc7-Tgfbr3 4:intron:E130114P18Rik 4:intron:Papola 4:intergenic:Gfra2-Gm9192 4:intergenic:Smpdl3a-AW822073 4:intron:Tmem19 4:intron:Il31ra 4:intron:Wipi1 4:intergenic:Zscan4c-Zscan4d 4:intergenic:Gm6133-Gm26430 4:exon:Colq 4:intergenic:Gm16046-Tcte2 4:intron:Pde4d 4:intron:Kcnma1 4:intergenic:Sntg2-Gm25865 4:intergenic:Ccdc125-Ccdc125/Cdk7 4:intergenic:Tmem108-Nphp3 4:intergenic:Gm13801-Lrrc4c 4:exon:Metrn/Gm26694 4:intergenic:Plxdc2-Gm24933 4:intergenic:Sptlc2-Alkbh1 4:intergenic:Gm23036-Gm22034 4:intergenic:Rpl17-ps4-Tnc 4:intergenic:Gm10036-Gm24228 4:intergenic:Letm1-Whsc1 4:intergenic:Fkbp5-Gm22146 2:intergenic:Gm15443-Gm23535 4:intergenic:1700060C16Rik-Sox5 4:intergenic:Ino80c-Galnt1 4:intron:Myo1d 3:intergenic:Gm12753-Gm12751 4:intergenic:C030013C21Rik-Myo1d 4:intergenic:Npffr1-Ppa1 3:intergenic:Zswim6-Gm22118 4:intron:Tpm3 4:intron:Foxo3 4:intron:Slc9a9 4:intron:Spock1 4:intergenic:Gm26354-Gm7536 4:intron:Pdzrn4 4:intergenic:Cdh6-Gm9630 4:exon:Ifih1 4:intergenic:Slc24a4-Rin3 4:intergenic:Gm13191-Frmd4a 4:intron:Vps13c 4:intron:Dcaf8 4:intergenic:Hspa4-Zcchc10 4:intergenic:Cnot1-4930513N10Rik/Cnot1 4:intergenic:Hoxc12-Hotair/HOTAIR_2 3:intergenic:Hsfy2-Satb2 3:intergenic:Gm25103-Dph6 4:intergenic:C130026L21Rik-Gm26897 4:intron:Gm5665 4:intron:Mbd5 4:intron:Apobec3 4:intergenic:Gm23041-Nav3 4:intron:Slc9a9 4:intergenic:Slc35f4-Gm22116 4:intron:Vav3 2:intergenic:Gm23459-Gm24634 3:intergenic:Impg2-Abi3bp 3:intron:Arv1 3:intergenic:Thsd7b-Gm16081 3:intergenic:Sec16b-Brinp2 4:intron:Atf6 4:intergenic:Gm15501-Gm5899 4:intergenic:Gm3993-Gm10479 4:intron:Il12rb1 4:intergenic:Ftl2/Mir692-2a-Grik3 4:intergenic:Gm5784-Gm6802 4:intron:Il1rap 4:intergenic:4930517O19Rik-Gm26367 4:intergenic:Neurod6-Ccdc129 4:intergenic:Neil3-Vegfc 4:intron:Ino80 4:intron:Arl5a 4:intron:Taok1 4:intron:C8b 4:intergenic:Wdpcp/Mdh1-Wdpcp 4:intron:Sntg1 4:intron:Gabrg3 4:intergenic:4933423P22Rik-Ptprj 4:intergenic:Cdh26-Gm14314 4:intergenic:Gm6802-Gm9257 4:intergenic:Mcoln2-Lpar3 4:intron:Pthlh 4:exon:Gm12441 4:intergenic:Agtr1b-Gm7488 4:intergenic:Gm23644-Gm15442 4:intergenic:Gm8421-Gm23945 4:intron:Tipin 4:intron:R3hdm2 4:intergenic:Nudt10-Bmp15 4:intergenic:Gm25144-Gm23147 4:intergenic:Pcdh17-Gm23926 4:intergenic:Olfr1215-Olfr1216 4:intron:Adam17 4:intergenic:Gm23240-Gm8357 4:intron:Fabp12 4:intron:Usp30 4:intergenic:Gm23591-Speer2 4:intron:Ccdc127 4:intergenic:Gm17391-2410018L13Rik 4:intergenic:Lrrc52-Rxrg 4:intergenic:Gm14081-Kif16b 4:intergenic:Gm17330-9030624G23Rik 4:intron:Pja2 4:intergenic:Gm22939-Cntnap2 Off-target primers show all... show less...
314 / rev	`AATAAGCAATTACTATTCTT TGG` `.................... ...` Inefficient Low GC content Cloning / PCR primers	70	84	19	19	58	77	0 - 0 - 2 - 31 - 188 0 - 0 - 0 - 3 - 3 221 off-targets	4:intron:Sgms1 4:intergenic:Rpl21-ps14-n-R5s93 4:intergenic:Gm23891-1700018B24Rik 4:intergenic:Gm24246-Pik3c3 3:intergenic:mmu-mir-6356-4930546C10Rik 3:intergenic:Gm24349-1700019A02Rik 4:intron:Nfia 4:intergenic:Cntnap5a-Gm23393 4:intergenic:Cntn5-Gm25365 3:intron:0610012H03Rik 4:intron:Edil3 3:intergenic:Gm20484-Nkx6-1 4:intron:E130307A14Rik 3:intergenic:Taar8b-Taar8c 4:exon:B230369F24Rik 4:intron:Hdgfrp3 4:intergenic:Gm14120-Gm25866 4:intergenic:Smoc2-Thbs2 4:intron:Rab8b 3:intron:Tnpo1 4:intergenic:Irx2-Irx4 4:intergenic:Rps24-ps2-Gm8775 4:intergenic:Zwint-Pcdh15 3:intergenic:Gm21596-Gjd2 3:intron:Lama2 3:intergenic:Gm4810-Cntnap3 4:intergenic:Cdh6-Gm9630 4:intergenic:Gm15394-Gm24547 4:intergenic:Gpr149-Gm22433 4:intergenic:Olfr721-ps1-Slc4a7 4:intergenic:Phip-RP23-13G1.6 4:intergenic:Gm362-Gm14580 4:intergenic:Shisa9-Gm6327 4:intergenic:Syndig1-Gm14140 4:intergenic:DLG2-AS1_1-Gm24552 3:intergenic:Cntn5-Gm25365 4:intron:Dpy19l4 4:intergenic:Tnfrsf11a-Gm26214 4:intergenic:Gm22217-Gm22448 4:intron:Grm1 4:intergenic:Gm13692-Gm13702 3:intron:Gmds 3:intron:Tyw5 4:exon:Nkx2-2 4:intergenic:Gm13018-Prdm2 4:intergenic:Gm16330-Npy5r 4:intergenic:3830408C21Rik-Apoo-ps 4:intron:AV099323 3:intergenic:Gm23892-Gm25382 3:intron:Clpx 4:intergenic:Hdac4-Ndufa10 4:intergenic:Tmprss15-Rps19-ps12 4:intron:Csmd3 4:intron:Shroom4 4:intergenic:Gm24285-4930467K11Rik 4:intergenic:Gm11779-Gm11782 4:intergenic:Gm8226-Gm26126 4:intergenic:Fam49a-5S_rRNA 4:intron:Kcnip4 4:intergenic:Gm22500-Igsf11 4:intergenic:n-R5s30-Rbfox1 4:intergenic:Rap1a-Adora3 4:intergenic:Gm14574-Gm14576 4:intergenic:Gm24908-Gm11836 4:exon:Prpf39 4:intergenic:Gm23041-Nav3 4:intergenic:Gm14776-Il1rapl1 4:intergenic:Gm22546-Gm22194 3:intron:Gm12695 4:intergenic:Gm9843-Usp25 4:intergenic:Gm26126-Gm11114 4:intergenic:Gm26028-Gm23576 4:intergenic:Slc7a13-Atp6v0d2 4:intron:Alk 4:intergenic:Gm23342-Myt1l 4:intergenic:Sh3rf3-AC153944.1 4:intergenic:2900079G21Rik-AC113095.1 4:intergenic:BC023105-Smim3 4:intergenic:Gm23272-4930579D09Rik 4:intergenic:Pcdh17-Gm23926 4:intergenic:Abcd3-Arhgap29 4:intergenic:n-R5s94-Gm1840 4:intergenic:Rpusd3-Cidec 4:intergenic:Sema6a-Gm5506 4:intron:2210408I21Rik 4:intron:Lclat1 4:intergenic:Gm24189-Ncoa7 4:intergenic:Gm10192-Gm12696 4:intergenic:Gm13634-Rapgef4 4:intron:Astn2 4:intron:Gm11393 4:intron:Hs3st5 4:intergenic:Gm23180-Gm22977 4:intergenic:Pdzd2-Gm24135 4:intron:2310035C23Rik 4:intron:Sorcs3 4:intergenic:Rab21-Tmem19 3:intergenic:Lrrc31-Samd7 4:intergenic:Lrrn1-Setmar 4:intergenic:Gm15385-Vbp1 3:intron:Gldc 3:intergenic:Nrg1-Gm26578 4:intron:Sil1 4:intron:Tmprss11a 3:intron:Cul3 3:intergenic:Pcnx-n-R5s62 4:intron:Ank3 4:intergenic:Gm15496-Kcnmb2 4:intergenic:Msln-Narfl 4:intergenic:Zwint-Pcdh15 4:intergenic:Plekhf2-Ndufaf6 4:intergenic:Sirpb1c-Gm5150 4:intergenic:Gm5803-Gm19840 4:intergenic:Satb1-RP24-465O15.1 4:intergenic:Gm22536-Gm6632 4:intergenic:Gpr27-Prok2 4:intron:Mdga1 4:intron:Cnot6l 4:intergenic:Gm12004-4930554G24Rik 4:intergenic:Pcsk2-Bfsp1 4:intergenic:Gm26291-Gm17377 3:intergenic:4930579O11Rik-Inpp4b 4:intergenic:Gm12086-Gm12087 4:intron:Fut9 4:intergenic:Gm24948-Nova1 4:intron:Kcnk5 4:intergenic:Agmo-Dgkb 4:intergenic:Mir3072/Mirg-Dio3os 3:intron:Nrxn3 4:intergenic:Gm24120-Nr2f2 4:intergenic:1700018B24Rik-Gm23511 4:intergenic:Gm24202-Gm8100 4:intergenic:Faim2-Gm5808 4:intron:Nrxn1 4:intergenic:n-R5s193-Spsb1 4:intergenic:Bpifa2-Gm21850 4:intron:Itga1 4:intergenic:RP23-13G1.6-Hmgn3 3:intergenic:Gm7535-Fpr3 4:intron:Slc9a9 4:intergenic:Kif26b-Gm24405 4:intron:Gm12796 4:intron:Gprasp1 4:intergenic:Wdr11-Gm23847 4:intergenic:Gm25578-Actr3 4:intergenic:Gm22360-Gm24586 4:intron:Cntn6 4:intergenic:Gm26354-Gm7536 4:intergenic:Fnd3c2-Gm14852 4:intergenic:Hdac9-4921508M14Rik 4:intron:Gm12278 4:intergenic:Gm22143-Gm7820 4:intron:Clstn2 4:intergenic:Phkb-Gm23904 4:intergenic:Gm11990-Pkd1l1 4:intergenic:Gm23013-Ppp1r3a 4:intron:Scml2 4:intron:Uhrf1bp1l 4:intergenic:Gm25437-Gm22684 4:intergenic:Gm14628-Gm14604 4:intergenic:Npvf-G930045G22Rik 4:intron:Sdk2 4:intergenic:BC030500-Gm24847 4:intergenic:Gm12121-Foxi1 3:intron:Wt1 4:intergenic:Ank1/Gm15816-Nkx6-3 4:intron:Phka2 4:intergenic:Gm26146-Gm8226 4:intergenic:mmu-mir-6336-Lrp1b 4:intergenic:Vmn2r98-Vmn2r-ps119 4:intergenic:Plekhb2-Gm25096 4:intergenic:Gm25078-Gm14653 3:intron:Esrrg 3:intergenic:Zwint-Pcdh15 4:intergenic:Gm22227-Zfp703 4:intron:Zdhhc15 4:intergenic:Epha3-Csnka2ip 4:intron:Fryl 4:intergenic:Gm11211-Gm24117 4:intron:Pcdh9 4:intergenic:D130040H23Rik-Gm10033 4:intergenic:Olfr924-Olfr26 3:intron:Ccser1 4:intergenic:Gm4906-Gm14515 2:intron:Cep57l1 4:intron:Cd209a 4:intron:Gabrr3 4:intergenic:Gm22067-Ep300 4:intergenic:4930467D21Rik-Antxr2 4:intergenic:Gm22273-Gm24079 4:intron:Zdhhc9 4:intergenic:Cenpw-Gm22623 4:intergenic:4930481B07Rik-Psmd4 4:intergenic:Slc22a22-Zhx2 4:intergenic:Gm14390-Gm14295 4:intergenic:Maf/Gm15655-Dynlrb2 4:intergenic:Gm23773-Gm5496 3:intergenic:Gm4070/Gm20663-Gm4070 3:intron:Gvin1 4:intergenic:Gm8840-Ikzf2 4:intergenic:Hs3st4/Gm27040-Gm25759 4:intergenic:Adamts18-Nudt7 4:intergenic:Gm11223-Gm11224 4:exon:Dgkk 4:intergenic:Gm10258-Mecom 4:intron:Srgap1 4:intergenic:Kctd16-Rps19-ps13 4:intergenic:Dusp10-Gm23349 2:intergenic:Gm22815-Cntn5 4:intron:Kdm4c 4:intergenic:Cdh6-Gm9630 3:intergenic:Gm9824-Gm9996/Soga3 4:intergenic:Gm18367-Gm20713/Gpc5 4:intergenic:Gm11404-Gm11221 3:intron:Ugt2b35 4:intergenic:Fam98a-Gm24126 4:intergenic:5033428I22Rik-Scrg1 4:intergenic:Gnai1-Gm17037 4:intron:Bcr 4:intergenic:Zfp521-Gm5160 4:intergenic:Gm27023-Gm25800 Off-target primers show all... show less...
158 / fw	`ATGACTGGCCTAACTTGAGC TGG` `.................... ...` Enzymes: AluBI, SmoI, BpuEI Cloning / PCR primers	68	90	47	2	69	74	0 - 1 - 13 - 16 - 77 0 - 0 - 0 - 0 - 0 107 off-targets	4:intron:Slc24a1 4:intergenic:4933402E13Rik-Gm14679 4:intergenic:Gm7219-Gm15035 4:intergenic:Gm23041-Nav3 4:intergenic:Etv6-Bcl2l14 2:intergenic:Dip2c-Zmynd11 3:intergenic:Cyp51-Gm9897 3:intergenic:Gm15638-Plcxd2 2:intergenic:Gm22031-Gm4823 3:intron:Pard3b 4:intron:0610012H03Rik 4:intergenic:Ccdc175-Rtn1 4:exon:Wwox 4:intergenic:Gm10782-Slc25a48 4:intergenic:Cadm2-Gm24681 4:intergenic:Gm24595-Maged1 4:exon:Gm14827 4:intergenic:Gm6447-Gm15062 3:intron:D11Wsu47e 4:intron:4930548K13Rik 4:intron:Gabrr2 4:intergenic:Ankrd1-Pcgf5 4:intergenic:Gm24728-Lmo4 4:intron:Opcml 3:intron:Ugt8a 3:intergenic:Gm13811-Gm13813 4:intergenic:Gm20813-Gm21922 4:intergenic:Gm21752-Gm21477 4:intergenic:Stim2-Gm24235 4:intron:Tusc5 4:intron:Vsx2 4:intergenic:Gm9726/mmu-mir-8099-2-Gm26055 4:exon:BC018473 4:intergenic:Wasl-Hyal6 4:intron:Srbd1 2:intergenic:Gm26131-Klhl13 4:intron:Ankrd61 4:intron:Prkaca 2:intergenic:Kcnh7-Gm23503 4:intergenic:Gm6756-Pcdha2 3:intergenic:Col11a1-Gm23750 4:intron:Zbtb7c 4:intergenic:Gm9578-Gm22891 4:intergenic:Oxct1-Plcxd3 2:intergenic:H2-M1-Gm8877 4:intergenic:Mir5099-Meox2 4:intergenic:Grin3a-Gm12458 4:intergenic:Gm22816-Cxcl2 4:exon:Amy1 4:intergenic:Rgn-Gm14539 4:intergenic:Mc5r-Mc2r 4:intergenic:Gm5152-Gm17091 4:intergenic:C1ql1-2410004I01Rik 4:intergenic:Gm25813-Gm23666 4:intron:Rit2 4:intergenic:Tgif2-5430405H02Rik 4:intergenic:Gm20828-Gm20812 4:intergenic:Gm20822-Gm21809 4:intron:Masp2 4:intron:Kcnn3 4:exon:Tmprss9 3:intron:Rnaseh2b 2:intron:Bmper 1:intergenic:Col22a1-Gm23217 4:intergenic:Gm22315-Gm16551/Gm20649 4:intron:Arhgef7 3:intergenic:Shisa6-Gm12296 3:intergenic:Gm24246-Pik3c3 4:intergenic:Tbc1d1-Gm25306 3:intron:Cdh7 4:intergenic:Ttll7-Gm23131 2:intergenic:Veph1-Shox2 2:intergenic:Oca2-Gabrg3 2:intron:Rfx3 2:intergenic:Gm11835-Gm11814 2:intergenic:Gm25202-Gm14505 3:intron:Prcc 4:intron:Acad11 4:intron:Cdh23 4:intergenic:Figla-Add2 2:intergenic:Gm27039-Adcyap1 4:intron:Hydin 4:exon:Zfp110 4:intron:Pls3 4:exon:Serinc1 3:intergenic:Gm26341-Amd2 4:intergenic:Rnasel-Rgsl1 4:intron:Arhgef4 2:intergenic:Gm13921-Ldlrad3 3:intergenic:Gm9001-Gm22283 4:intergenic:Gm4654-Scel 3:intergenic:Slc25a36-Rpl7a-ps10 4:intron:Ubn2 4:intergenic:Erbb4-Gm16076 4:intergenic:Grrp1-Pdik1l 4:intron:Sema3a 4:intergenic:Gm26685-Dpt 4:intergenic:Gm9921-Gm24948 4:intergenic:Gm25722-Gm10051 3:intergenic:Vmn2r-ps117-Vmn2r97 4:intron:Wnt4 4:intergenic:Vmn2r89-Gm7247 4:intergenic:Gm7191-Gm23494 4:intergenic:Tmem18-Fam150b 4:intron:Vmn2r13 4:intergenic:Gm7327-Gm6743 4:intergenic:Olfr1257-Olfr1258 Off-target primers show all... show less...
110 / fw	`CCTTAAACTCAGAATTAAAC TGG` `.................... ...` Enzymes: BmuI, Bse1I, Tru1I Cloning / PCR primers	67	83	25	-3	65	79	0 - 0 - 1 - 24 - 246 0 - 0 - 0 - 2 - 5 271 off-targets	4:intron:Otogl 4:intergenic:Usp46/1700094C10Rik-1700112M01Rik/Usp46 3:intergenic:Gm5943-Gm14905 3:intron:Atp8b4 4:intergenic:Olfr551-Olfr552 4:intergenic:Olfr1169-ps1-Olfr1170 4:intron:Slc17a5 4:intergenic:Gm13364-Gm6587 4:intergenic:Fnip1-Rapgef6 4:intergenic:Gm26662-Kirrel3 4:intron:Ptplb 3:intergenic:E430024P14Rik/Tagap-Rsph3a/E430024P14Rik 4:intron:Hmgxb4 3:intron:Fndc3c1 4:intergenic:Zswim6-Gm22118 4:intron:Cadps 4:intron:Sox5 4:intergenic:Rps19-ps12-Gm23083 4:intron:Eml1 3:intron:Cwc27 3:intergenic:AC100386.1-Gm8394 2:intron:Ednrb 4:intergenic:Gm22186-Gm22236 4:intron:Sspo 4:intergenic:Mkx-Armc4 4:intron:Gabpa 4:intergenic:Ncoa2-Tram1 4:intron:Ppp2r5c 4:intergenic:Inpp4b-4930579O11Rik 4:intergenic:Gm12020-Gm12022 3:intergenic:Mtpap-9430020K01Rik 4:intron:Crebbp 4:intron:Ccdc68 4:intergenic:Tctn1-Pptc7 4:exon:Zfp839 4:intron:Mthfd2l 3:intergenic:Gm24591-Gm26241 4:intergenic:Gm5576-Reg3b 4:intergenic:Eif3s6-ps2-Gm25418 4:intron:Hpse2 4:intron:E130114P18Rik 4:intergenic:Gm23018-Mettl21d 3:intergenic:Gm3763-Gm14370 3:intergenic:Cacng2-Cacng2/A730060N03Rik 3:intergenic:Gm26291-Gm17377 4:intergenic:St8sia2-AC114591.1 4:intron:Dlgap1 4:intergenic:Slamf1-Cd84 4:intron:Ano1 4:intergenic:Gm6531-Olfr371 4:intergenic:Mtpap-9430020K01Rik 3:intergenic:Dmd-Gm14770 4:intergenic:Gm12914-Gm11264 4:intron:Acsl5 4:intergenic:Gm15157-Sat1 3:intergenic:Chmp2b-Vgll3 4:intergenic:Gm7091-Vsig1 4:intergenic:Gm13836-Pxn 4:intergenic:RP24-108J19.1-Lrrc6 4:intergenic:Mc2r-mmu-mir-6356 4:intron:Peli2 4:intergenic:Tshz3-Gm24550 4:intergenic:Gm12363-Zfp292 4:intron:Actl6a 4:intron:Cngb3 4:intron:Sfxn5 4:intergenic:Gm10248-Kcnma1 4:intron:Mipep 4:exon:Gm16223 4:intergenic:4931431F19Rik-4930516K23Rik 4:intergenic:Gm6977-Gm44 4:intergenic:Gm16505-2810429I04Rik 4:intergenic:Gm16038-Gm2635 4:intergenic:Gm16025/Gm7281-Gm2666 4:intergenic:AC168977.2-AC168977.1 4:intergenic:G530012D18Rik-Sp110/G530012D18Rik 4:intergenic:Gm15433-C130026I21Rik 4:intergenic:1700060C16Rik-Sox5 4:intron:Erg 4:intergenic:Ino80c-Galnt1 4:intron:Apobec3 4:intron:Trerf1 4:intergenic:Ahctf1/Gm10518-Cdc42bpa 4:intergenic:Gm25060-Galr1 3:intergenic:Gm25935-Gm22593 4:intron:Jmy 4:intergenic:Gm15323-Ankrd55 4:intergenic:Hoxc12-Hotair/HOTAIR_2 4:intergenic:Zdhhc25-Brd1 4:intergenic:Ticam2-Tmed7 4:intron:Lancl3 3:intergenic:Rgs3-Gm11209 4:intron:Slc2a13 4:intergenic:Gm25442-Gm25696 4:intron:Scaper 4:intergenic:Gm13255-Gm13217 4:exon:Neat1/NEAT1_3/NEAT1_2/NEAT1_1 4:intergenic:Ddx3y-Usp9y 4:intron:Tbc1d32 3:intergenic:Gm22544-Gm22509 4:intergenic:Fbxo15-Gm25453 4:intergenic:Gm14914-Gm14917 3:intergenic:Fshr-Gm10184 4:intergenic:Cntnap5b-Gm25537 4:intergenic:Cat-Gm13996 4:intron:Efnb2 4:intron:Arv1 3:intergenic:Clstn2-2610303G11Rik 4:intron:Klhl31 4:intron:Kif21a 4:intergenic:Abcc3-Cacna1g 4:intergenic:4933434M16Rik-2610035D17Rik 4:intergenic:Triml2-Zfp42 4:intergenic:Pcsk2-Bfsp1 4:intergenic:Trim63-Gm13195 4:intron:Aox1 4:intergenic:Gm26182-Gm14929 4:intergenic:Gpr114-Gm10286 4:intron:Foxo3 4:intergenic:Gm26580/Kcnq5-Rims1 4:intron:Etv6 4:intron:Zfpm2 4:intron:Cask 4:intergenic:Hcn1-Gm26349 4:intron:Specc1 3:intergenic:Mcu-Micu1 4:intron:Igsf23 4:intergenic:Gm23594-Il20rb 4:intergenic:Gm13871-Gm11756 4:intergenic:Gm11757-Gm13871 4:intergenic:Gm11758-Gm11757 4:intergenic:Gm22939-Cntnap2 4:intergenic:Thrb-Gm24140 4:intergenic:Gm22211-Gm6181 4:intergenic:Atg10-A830009L08Rik 4:intergenic:Gm5620-Zic1 4:intron:Vrk3 4:intergenic:Vezt-Vezt/Mir331 3:intron:Fry 4:intergenic:Gm15031-Gm25915 4:intergenic:Sox6-1700003G18Rik 4:intron:Caln1 4:intron:Pde4d 4:intergenic:Col4a3-n-R5s213 4:intergenic:Gm11816-Clvs1 4:intergenic:Rps19-ps6-Tecpr2 4:intergenic:Gm11676-Gm11677 4:intergenic:Sptlc2-Alkbh1 4:intergenic:Rps10-ps2-Gm9697 4:intergenic:Gm8100-Gm26795 4:intergenic:Gm5611-Chordc1 4:intron:Fbxl20 4:intron:Cbx7 4:exon:Zfp94 4:intergenic:Gm13103-Pramef6 4:intergenic:Gm13023-Gm13084 4:intergenic:Gm13078-Gm13087 4:intergenic:Gm13086-Gm13089 4:intergenic:Tex261-Nagk 4:intergenic:Gm4746-Gm6977 4:intron:Dnah5 4:intergenic:Gm10136-Cdc37l1 4:intergenic:Gm22444-mmu-mir-6238 4:intergenic:Oog2-C87977 4:intergenic:Gm11237-Gm11236 4:intergenic:Gm6369-Armc1 4:intergenic:Slco6c1-D1Ertd622e 4:intergenic:Gm24836-Sde2 4:intergenic:Gm9697-2310042E22Rik 4:intron:Ccnc 4:intron:Rreb1 4:intron:Sclt1 4:intron:2810032G03Rik 4:intergenic:Gm6024-6030498E09Rik 4:intergenic:Lrp1b-mmu-mir-6336 4:intergenic:Gm15946-Wtap 4:intergenic:C130026L21Rik-Gm26897 4:intergenic:Plxdc2-Gm24933 4:intron:Chuk 4:intergenic:Sh3tc2-Adrb2 4:intron:Tmem117 4:intron:Fxyd6 4:intron:Rbl1 4:intron:Abcb5 4:intergenic:Gm5496-Gm4518 4:intergenic:Epha5-Gm24626 4:intron:Pecam1 4:intron:Lima1 4:intron:Cbx5 4:intergenic:Gm14624-Gm22103 4:intergenic:Ctsq-Ctsr 4:intron:Lnp 4:intergenic:Gm24078-1700025O08Rik 4:intergenic:Gm26235-Gm25392 4:intergenic:Gm9002-Kifc5c-ps 4:intergenic:Gm26845/9630033F20Rik-Ccnd2 4:intergenic:Ap1m1-Gm10282 4:intergenic:Gm22628-5033411D12Rik 4:intergenic:Gm25591-Syt1 4:intron:Zfp91 4:intergenic:Gm24767-Gm17733 3:intergenic:Gm24963-Gm10742 4:intergenic:Wwox-Gm23677 4:intergenic:Clstn2-2610303G11Rik 4:intron:Rcor1 4:intergenic:Neto1-Cbln2 4:intergenic:Cdh9-Gm22032 4:intergenic:Gm23640-Wipf2 4:intron:Fam129b 4:intron:Nudt3 4:intergenic:Mki67-Gm9341 4:intergenic:Klrg1-Gm25304 4:intron:Srp19 4:intergenic:Mtpap-9430020K01Rik 4:intergenic:Gm25522-Gm25587 4:intergenic:Tpx2-Gm24421 4:intron:Adam17 4:intron:Gm16386 4:intron:Ext1 4:intron:St18 4:exon:Gm13437 4:intron:Foxp1 4:intron:St6galnac6 4:intergenic:Stim2-Gm24235 4:intergenic:n-R5s209-Gm7846 4:intron:Magi2 4:intron:Elp6 4:intron:Dock7 4:intron:Gm15908 4:intergenic:Gm25005-Gm22819 4:intron:Sycp2 4:intergenic:Gm15425-Pde10a 4:intergenic:Gpr27-Prok2 4:intergenic:Lcorl-Gm7931 4:intron:Nlrc3 4:intergenic:Adam28-Stc1 4:intergenic:C87414-Gm16429 4:intergenic:Gap-Gm7792 4:intergenic:AC124606.1-Gm7792 4:intergenic:AC126035.1-Gm16367 4:intergenic:Gm16427-C87414 4:intergenic:AC140365.1-AC124606.1 4:intergenic:Gm6205-Gm3183 4:intergenic:AC123873.1-Gm7792 4:intergenic:Gm7942-Gm16513 4:intergenic:D5Ertd577e-Gm3286 4:intron:Il5ra 4:intergenic:Gm25602-Tbc1d32 4:intron:Slc9a9 3:intergenic:Gm11747-Gm11748/Engase 4:intergenic:Fmod-Btg2 4:intron:B4galnt2 3:intron:Brinp2 4:intergenic:Gm22104-Gm25550 4:intron:March11 4:intron:Rad54b 4:intergenic:Phyhipl-4930533K18Rik 4:intergenic:Trim27-Gpx5 4:intron:Scn1a 4:intergenic:Gm13856/Slc35b4-Slc35b4 3:intron:Ppm1d 4:intergenic:Gm14461-Gm14460 4:intron:2700049A03Rik 4:intron:Ehmt1 4:intergenic:Gm25300-Gm26903 4:intergenic:Zscan30-Zfp35 4:intron:Fam53a 4:intron:Erbb4 4:intron:Bcas3 4:intergenic:Wasl-Hyal6 4:intergenic:Mir3963-Eltd1 Off-target primers show all... show less...
406 / rev	`CCAGTCTTTAGTTTATTACC TGG` `.................... ...` Enzymes: BcoDI, BspTNI, CsiI, BstNI, StyD4I, LpnPI Cloning / PCR primers	66	87	43	2	58	77	0 - 1 - 2 - 11 - 105 0 - 0 - 0 - 0 - 2 119 off-targets	1:intergenic:Gm24510-Nrxn1 4:intron:Pecam1 4:intergenic:Bms1-Zfp248/Gm25953 4:intron:Macrod2 4:intron:Copg2 4:intron:Otol1 4:exon:Rab4b/Gm21983 4:intergenic:Gm27025-Gm25700 4:intron:Adcy9 4:intergenic:Cdh6-Gm9630 4:intergenic:Gm13925-Gm20638 2:intron:Gm872 3:intron:Nfia 4:intergenic:Unc13c-Gm25105 4:intergenic:Gm23881-Gm3534 4:intergenic:Gm25598-BC002059 4:intergenic:Bbs10-Nap1l1 4:intergenic:Tle4-Gm26026 4:intergenic:Gm10259-Nlgn1 4:intergenic:Gm5334-Arrdc4 4:intron:Nalcn 4:intron:Prkce 4:intergenic:Gm23013-Ppp1r3a 4:intergenic:Gm9274-Pcdh20 4:intergenic:Extl2-Gm26344 3:intron:Sorcs3 4:intergenic:Igkv15-103-Igkv14-100 4:intergenic:Eepd1-Gm25861 4:intergenic:Fut10-Gm23539 4:intergenic:Thumpd2-Slc8a1 4:intergenic:Gm11919-Gm11923 4:intergenic:Gm831-Gm12962 4:intergenic:Gm4822-Tpm3-rs7 4:intron:Spen 4:intergenic:Gm23926-Gm25115 4:intron:C530008M17Rik 4:intron:Sycp2 4:intergenic:Tnfsf4-Tnfsf18 4:intron:Tmem131 4:intron:Tnpo3 4:intron:Phkg1 4:intergenic:Gm24790-Klf6 4:intron:Lingo2 4:intergenic:Rnpc3-Col11a1 4:intron:B4galt6 4:intergenic:Slc14a2-Gm6133 3:intergenic:Sult2a2-Sult2a1 4:exon:N28178 4:intergenic:Dusp10-Gm23349 3:intron:Nckap5 4:intron:Zfp385b 4:intergenic:Gm26031-Gm9801 4:intergenic:AC161410.1-Haus8 4:intergenic:Fcho1-Zfp709 4:intergenic:Gm9423-Depdc1a 3:intron:Pbrm1 3:intron:Col6a6 4:intergenic:Zswim5-Gm26355 4:intron:Actr3 4:intron:Chrna7 4:intergenic:Slc2a2-1700112D23Rik 4:intergenic:Gm13809-Gm13808 4:intron:Prkch 4:intron:Col5a1 4:intron:Trio 4:intergenic:Defb11-Defb15 4:intron:1700088E04Rik 4:intergenic:Gbp2-Gbp2/mmu-mir-6381 4:intron:Adk 4:intergenic:Gm13211-Gm13254 4:intergenic:Gm22913-Gm20091 4:intron:Atp8a2 4:intergenic:Cyp3a41a-Cyp3a44 4:intron:Kcnh5 4:exon:Zfp704/C030034L19Rik 4:intergenic:Gm9192-Gm23735 4:intergenic:Gm6369-Armc1 4:intergenic:Zfp423-Gm2716 4:intergenic:Gm24304-Gm22974 4:intergenic:Atg3-Gm6030 4:intergenic:Paqr3-Gm5560 4:intron:Grik2 4:intergenic:Tmcc3-Gm16154 4:intron:Sema5a 4:intron:Plac1 4:intron:Mthfd1l 4:intron:Pappa2 4:intron:Aqr 3:intergenic:Kcnc2-Atxn7l3b/Gm26596 4:intergenic:Gm4725-Gm9498 2:intron:Msn 4:intron:Dock2 4:intergenic:Mycn-Gm24208 4:intergenic:Gm11761-Rptor 4:intron:Sltm 4:intergenic:Arap3-Pcdh1 4:intergenic:Clasp1-Gm26080 4:intergenic:Syndig1-Gm14140 4:intergenic:Gm9800-Fhit 4:intergenic:Gm5916-RP23-319C8.4 4:intergenic:Htr1a-Dph3b-ps 4:intergenic:Gm23979-Gm22845 4:intergenic:Ifng-Dyrk2/4932442E05Rik 4:intergenic:Asph/Clvs1-Asph 4:intron:Abcf1 4:intergenic:5033430I15Rik-Gm10113 4:intergenic:Gm15531-Mitf 4:intron:Actn2 3:intergenic:Speer4e-Gm10354 3:intron:Speer7-ps1 3:intergenic:Gm10354-Gm17019 3:intergenic:Gm17019-Gm21847 4:intergenic:Pgk1-rs7-Kcns3 4:intergenic:Ift81-P2rx7 4:intergenic:Gm21847-Speer4d 4:intergenic:Gm25186-Gm11379 4:intergenic:Nek10-Lrrc3b 4:intron:Fshr 4:exon:Lcp1 Off-target primers show all... show less...
427 / fw	`CAGGTAATAAACTAAAGACT GGG` `.................... ...` Enzymes: BmuI, Bse1I Cloning / PCR primers	64	80	56	56	63	76	0 - 0 - 3 - 23 - 183 0 - 0 - 2 - 0 - 6 209 off-targets	4:intergenic:Mmp10-Mmp8 4:intergenic:Gm26341-Amd2 4:intergenic:Gm11997-Vwc2 4:intergenic:n-R5s30-Rbfox1 4:intergenic:Nol10-Odc1 4:intergenic:Gm14103-Gm14094 4:intergenic:Gm11680-BC006965 4:intergenic:Gm24471-Cartpt 3:intergenic:Cyp3a41b-Cyp3a41a 4:intergenic:Cyp3a16-Cyp3a41b 3:intergenic:Gm14273-Fam210b 4:intron:Phlpp1 3:intergenic:Olfr1174-ps-Olfr1175-ps 4:intron:Prune2 4:intron:Ube2d2a 4:intron:9030622O22Rik 4:intergenic:Gm7719-Gm24377 4:intron:Slc22a28 4:intergenic:Snx19-Adamts15 4:intron:Cyba 4:intron:Rc3h1 4:intron:Cep112 4:intron:Arhgef10l 4:intergenic:Tmem179-Inf2 4:intergenic:Gm21847-Speer4d 4:intergenic:Speer4e-Gm10354 4:intron:Speer7-ps1 4:intergenic:Gm10354-Gm17019 4:intergenic:Gm17019-Gm21847 4:intron:Efcab6 4:intron:Kirrel3 4:intergenic:Gm24275-Gm12291 4:intergenic:Igkv6-13-Igkv3-12 4:intergenic:Gm24237-Gm25342 4:intron:Dcdc5 4:intergenic:Gm9578-Gm22891 4:intergenic:Gm25853-Gm23180 4:intergenic:Gm14218-Pak7 4:intron:Ttc3 4:intron:Atf7ip 4:intron:Ttll5 4:intergenic:Coro2b-Itga11 4:intron:Zc3h11a/Zbed6 3:intron:Fars2 4:intergenic:Jag1-Gm14055 3:intron:Nckap5 4:intergenic:Echdc3-A230108P19Rik 4:intergenic:D3Ertd751e-Gm25714 4:intron:Rnf4 4:intron:5730522E02Rik 4:intron:Epha3 4:intron:Cnksr3 3:intron:Galnt2 4:intergenic:Gm25830-4930555F03Rik 4:intergenic:Lipk-Lipn 4:intergenic:Gpc5-Gpc6 4:intergenic:Rad51/Rmdn3-Rmdn3 4:intron:Sorcs3 4:intron:Mesdc2 4:intergenic:Gm25843-Tmeff2 4:intron:Ube2b 4:intergenic:Dgkz-Creb3l1 4:intron:Kcnma1 4:intron:Rin2 4:intergenic:Nell1-Ano5 4:intergenic:Gm1818-Gm26454 4:intergenic:Gm7790-Gm14977 3:intergenic:Tmcc3-Gm16154 3:intron:Lrfn2 4:intergenic:Gm7069-Gm10649 4:intron:Hadha 4:intron:Mei4 4:intron:Dtna 4:intergenic:Gm14116-Nkx2-2 4:intergenic:Gm4725-Gm9498 4:intron:Cnot6l 4:intergenic:Pcdh17-Gm23926 4:intergenic:Gm7952-Slco6d1 4:intergenic:Gm14702-Gm14700 2:intergenic:Gm25659-Gm25644 4:intergenic:Gpr165-Gm16394 4:intergenic:Alg10b-Cpne8 4:intergenic:Daam1-Gpr135 4:intergenic:Nefl-Nefm 4:intergenic:Gm13419-Psmd5 3:intron:Tgfa 4:intergenic:Mir453-Mirg 4:intron:Fbxw9 4:intergenic:Rps6-ps2-mmu-mir-8110 4:intergenic:Nxph1-Gm24104 4:intergenic:Fam19a2-Gm23777 4:intergenic:Gm2862-Myo10 4:intergenic:Gm14519-Gm14518 4:intron:Nfia 4:exon:Mettl21e 4:intergenic:Gm24710-Gm24889 4:intergenic:Gm15908-Fam50b 4:intergenic:Gm12702-Ror1 4:intron:Ctnnd2 4:intergenic:Pnlip-Pnliprp1 4:intergenic:Gm853-Serinc2 4:intergenic:Mycn-Gm24208 4:intron:Prkcb 4:intron:Cd300e 4:intergenic:Bclaf1-Mtfr2 4:intergenic:Ptprq-Otogl 4:intergenic:5033430I15Rik-Gm10113 3:intergenic:Gm23440-Rapgef2 4:intergenic:Cyp3a41a-Cyp3a44 4:exon:Hmgb1 4:intron:Adarb2 3:intergenic:Gm27023-Gm25800 3:intergenic:Txndc12-Rab3b 4:intergenic:Tram1l1-Gm4617 4:intron:Tmem161b 4:intergenic:Gm13925-Gm20638 4:intron:Tex15 4:intron:Iqca 4:intron:Stx12 4:intron:Sos1 4:intergenic:Gm25537-Gm24122 4:intron:Dpyd 4:intergenic:Gm25132-Dclk1 3:intron:Strbp 4:intron:Kcnma1 4:intergenic:Gm24350-Gm13464 4:intergenic:Gm7146-Klhl4 4:intergenic:Gm12381-Gm25581 4:intergenic:1700058G18Rik-Kcnmb4 4:intron:Nr1h4 2:intergenic:Gm24510-Nrxn1 4:intron:Cntnap5c 4:intron:E130308A19Rik 4:intron:Grid1 4:intergenic:Gm5101-Slco6b1 4:intergenic:Adcy5-Gm25179 4:intergenic:Ebf1-Gm12159 4:intron:Fam210a 4:intergenic:Gm8919-Gm8935 4:intergenic:Gm26031-Gm9801 4:intergenic:Flrt2-1700019M22Rik 3:intergenic:Slc14a2-Gm6133 4:intergenic:Gm24237-Gm25342 4:intergenic:Gm15067-Gm15066 4:exon:Chd8 4:intergenic:Gm10660-Nat1 3:intergenic:Gm23276-Dach1 4:intron:Phkg1 4:intergenic:Gm16685-Gm24614 4:intergenic:Zfp516-Gm24720 4:intergenic:Gm12509-Gm12508 4:intron:Sufu 4:intergenic:Zfp423-Gm2716 2:intergenic:Gkn3-Bmp10 4:intergenic:Gm24070-Gm24994 4:intergenic:mmu-mir-6344-Irs1/Gm9747 4:intron:Rorc 4:intergenic:Gm20319-Gmnc 3:intergenic:Rock1-Greb1l 4:intron:Prkce 4:intergenic:Gm24691-Grm8 4:intergenic:Gm10710-Gm22342 4:intron:Ror2 4:intergenic:Npbwr1-Rb1cc1 4:intron:Astn2 4:intergenic:Gm7931-Slit2 4:intergenic:Atp1a1-Mab21l3 4:intergenic:Dennd5b-Gm10203 4:exon:Epb4.1l3 4:intergenic:Gm20491-4930480K15Rik 4:intron:Nrxn3 4:intron:Cpne4 4:intergenic:Cabp4-Gpr152 4:intergenic:1500009L16Rik-Nuak1 4:intron:Ss18 3:intergenic:Gm14680-Gm8244 3:intergenic:Gm1862-Gm14591 4:intergenic:Gm16129-Plch1 4:intron:Dmd 3:intron:Lpcat3 4:intergenic:Car10-Gm24856 4:intergenic:Gm16039-Gm24059 4:intron:1700057G04Rik 4:intergenic:Gm14161-Gm17416 3:intergenic:Gm13850-Plxna4 4:intergenic:D1Ertd622e-Ppip5k2 4:intergenic:Wac-Gm5819 4:intergenic:9630028H03Rik-Gm14074 4:intron:Dgkh 4:intergenic:6030471H07Rik-Gm12416 4:intergenic:Gm23538-1700110M21Rik 4:intergenic:Gm22882-Cxcl12 4:intron:Ints9 4:intergenic:Gm12413-Sh3gl2 4:intron:Foxm1 3:intergenic:Gm23509-Pcdh9 3:intergenic:Gm11350-Gm11351 3:intron:Gpr110 4:intron:Sema5a 4:intron:Nrcam 4:intron:Chm 4:intergenic:4930505H01Rik-Gm23769 4:intergenic:Pacsin2-Ttll1 4:intergenic:Gm5121-Ppcdc 4:intergenic:Gm14029-Slc20a1 4:intergenic:Gm24731-Gm22076 4:intergenic:Mcpt2-Mcpt4 4:intron:Itch 4:intron:Morc1 Off-target primers show all... show less...
346 / fw	`AATTGCTTATTAAAACAAGT AGG` `.................... ...` Low GC content Cloning / PCR primers	59	73	59	5	64	80	0 - 1 - 4 - 35 - 255 0 - 0 - 1 - 3 - 7 295 off-targets	4:intergenic:Olfr397-Olfr398 4:intergenic:Jmy-Bhmt 4:intergenic:Gm6771-Ptchd4 3:intergenic:Gm11784-Gm11785 3:intergenic:9230019H11Rik-H60c 4:intergenic:Med26-Smim7 4:intergenic:Fbxw4-Fgf8 4:intergenic:C130060K24Rik-Tnip3 4:intergenic:Gm5384-Chst7 3:intergenic:Gm12637-Gm22605 4:intergenic:Gm25261-Gm831 4:intergenic:Cmpk2-Gm9866 3:intergenic:Gm26674/Sash1-Gm9930 4:intron:Gm16332 4:intron:Cfhr2 4:intergenic:Gm24696-Myc 4:intergenic:Vat1l-4933408N05Rik 4:intergenic:Gm26913-Gm4076 4:intergenic:Foxp2-Mdfic 4:intergenic:Ppm1h-Mirlet7i 4:intergenic:Tcaim-Zfp445 4:intergenic:3830408C21Rik-Apoo-ps 4:intron:1700112E06Rik 3:intergenic:Fli1-RP24-88B13.1 4:intergenic:Gm22593-Mettl4 4:intergenic:Gm11404-Gm11221 4:intergenic:Vmn2r60-Vmn2r61 4:intergenic:Emc2-Tmem74 4:intergenic:B930078G14Rik-Fam155a 4:intron:Sh3kbp1 3:intron:Prkdc 3:intron:Ect2l 4:intergenic:Pi15-Gm16070 4:intergenic:Foxn3-Gm26839 4:intergenic:Gm22451-Sis 4:intergenic:Gm8508-Gm26779 4:intron:Slc15a2 3:intergenic:Gm23075-Gm22853 4:intergenic:4931417E11Rik-Gm26179 4:intergenic:Gm25437-Gm22684 4:intron:Fam110b 4:intergenic:Gm9630-Gm24080 3:intergenic:Gfra2-Gm9192 4:intergenic:Olfr1233-Olfr1234 3:intergenic:Gm14705-Aff2 4:intergenic:E230019M04Rik-Gm15046 4:intergenic:Pdgfc-Gm22531 4:intergenic:Gm25538-Gm17337 4:intergenic:Gm22331-Gm24937 4:intron:Cntnap5a 4:intergenic:Ptprd-Ptprd/B230208B08Rik 4:intergenic:Agbl1-Gm23239 3:intergenic:Tbkbp1-Kpnb1/Gm11583 4:intergenic:Gm14989-Gm24675 4:intergenic:Txndc5-Bloc1s5 2:intron:Pum2 4:intron:Celf2 4:intron:Cacna1e 4:intron:Ncbp2 4:intergenic:Nbea-Gm25727 4:intergenic:Il20ra-Gm25256 4:intergenic:Sgk1-Gm8540 4:intron:Ldlrad4 4:intergenic:Gm11222-Gm11223 4:intron:Fgd2 4:intergenic:Gm25935-Gm22593 4:intron:Ttc39c 4:intergenic:Gm22266-Gm24993 4:intron:Cacna2d1 4:intergenic:Gm26278-Mgat4c 4:intergenic:Fam20a-Gm11684 2:intergenic:Gm25344-Tmem232 4:intron:Carm1 4:intron:Fbxl17 4:intergenic:Gm23083-Gm24773 4:intergenic:Csmd1-Gm25665 4:intergenic:Kcnma1-Gm6158 3:intergenic:Ppef1-Rs1 4:intergenic:Gm22673-Gm23059 4:intergenic:Pabpc5-Gm4913 4:intergenic:Pdha1-Gm15195 4:intergenic:Gm24733-Gm807 4:intergenic:Gm23661-Gm23067 4:intergenic:Nox3-Gm23186 4:intergenic:Pcdh20-Gm25886 4:intergenic:Pla2g4e-Pla2g4e/Gm13997 4:intergenic:Gm22977-Zpld1 4:intergenic:Gm25319-Gm22070 4:intergenic:Gm23972-Ofcc1 4:intergenic:Gm12514-Gm12509 4:intergenic:Gm22507-Trhde 4:intergenic:Kbtbd8-AY512915 4:intergenic:Ier5-Mr1 4:intergenic:Gm14830-Rpl13-ps5 4:intergenic:Gm22360-Gm24586 4:intron:Necab1 4:intergenic:RP23-131O4.2-Gm23470 3:intron:Gab2 4:intergenic:Klhl14-Klhl14/4930426D05Rik 4:intergenic:Gm6377/Sh3bgrl-Gm14868 4:intron:Rbms1 4:intergenic:Dct-Gm26773 4:intergenic:Gm26972-Gm23581 4:intergenic:Zic3-Gm14649 4:intron:Nqo2 4:intergenic:Irx2-Irx4 4:intergenic:Gm22565-Gm24288 4:intergenic:Amy2-ps1-Amy2a5 4:intergenic:Caps2-Kcnc2 4:intron:Kcnma1 4:intron:Fam21 4:intergenic:Gm5614-Cdon 3:intergenic:Gm25101-Cdh12 4:intergenic:Eci2-Gm22674 4:intron:Zswim6 4:intron:Ppard 4:intergenic:Gm24250-Nlgn1 4:intergenic:Tpst1-Kctd7 4:intron:Fam73a 4:intergenic:Lingo1-Odf3l1 4:intergenic:Gm24434-Arl4a 4:intergenic:Gm22730-Pard3 4:intergenic:Mir466f-4-Irx1 4:intergenic:Gm7191-Gm23494 4:intergenic:Gm4746-Gm6977 4:intergenic:Gm14657-Gm8260 4:intergenic:Ctnna3-Gm22829 4:intron:Ptprt 3:intron:Ryr3 4:intron:Lrba 4:intergenic:Gm22074-Pkia 4:intergenic:Gpr149-Gm22433 4:intergenic:Lingo2-Gm12369 3:intron:Atp13a5 4:intron:Fam110b 4:intron:Ica1 4:intergenic:Slc7a11-Gm24503 4:intron:Ctnna3 4:intergenic:Hsfy2-Satb2 4:intergenic:Gm26076-Gm25519 4:intergenic:Gm24443-Gm22529 4:intron:Slc30a8 4:intergenic:Rnpc3-Col11a1 4:intron:Dpyd 4:intron:Erc2 4:intron:Macrod2 4:intergenic:Cdh6-Gm9630 4:intron:Atpaf1 4:intron:Myt1l 4:intron:Tspan11 3:intergenic:Gm23981-Ttc29 4:intergenic:Thumpd2-Slc8a1 1:intergenic:Gm9897-Fzd1 4:intergenic:Zfyve28-Gm15513 4:intergenic:Adamts2-Gm12199 4:intergenic:AC100386.1-Gm8394 4:intergenic:Prex2-A830018L16Rik 2:intergenic:Dlg2-Gm23928 4:intergenic:Gm22217-Gm22448 4:intergenic:Gm26256-n-R5s61 4:intergenic:Cdh12-AC125320.1 4:intergenic:Gm23154-Gm12604 4:intergenic:Gm23891-1700018B24Rik 4:intergenic:Gm23790-Gm5422 4:intergenic:Xirp2-mmu-mir-7224 3:intron:Sgms1 4:intron:Ogdhl 4:intergenic:Olfr282-Olfr281 4:intergenic:Gm21805-Gm21241 4:intergenic:Gm20474-Gm10330 4:intergenic:Olfr1458-Olfr1459 4:intergenic:Gm10330-Gm17330 4:intergenic:Crh-4632415L05Rik 4:intron:AY512915 3:intron:Nrxn3 4:intergenic:Gm11924-4930556G01Rik 4:intron:Lta4h 4:intron:Dpy19l4 4:intron:Sf3b2 4:intergenic:Cdh19-Dsel 4:intergenic:AC147224.1-Cdh12 4:intergenic:Efcab5-Ssh2 4:intergenic:Gm23684-Pnpla8 4:intergenic:1700084C01Rik/Gm15852-Hsd17b7 4:intergenic:Enpp1-Enpp3 4:intergenic:Tnn-Mrps14 4:intergenic:Gm11354-Gm11355 4:intergenic:4930555F03Rik-Gm23986 4:intron:Map2 4:intergenic:Gm22495-Dtna 4:intergenic:Gm25722-Gm10051 4:intergenic:Gm24177-Gm12484 3:intron:Cacna1c 4:intergenic:Zfp652-Phospho1 4:intergenic:Gm24921-Fscb 4:intron:St6gal1 4:intron:E130018O15Rik 4:intron:Styk1 4:intron:Itch 4:intron:Vmn2r62 4:intron:Vmn2r63 4:intron:Gm9268 2:intergenic:Gm24679-Gm22219 4:intergenic:Gm22939-Cntnap2 4:intron:Sox30 4:intron:Mbtps1 4:intron:Mbd5 3:intron:Gm11986 4:intergenic:Gm23492-Rasgef1c 4:intron:E230008N13Rik 3:intron:Atm 4:intergenic:Gm22966-Gm15443 4:intergenic:Gm20679-Vmn1r66 4:intergenic:Gm26715-2410141K09Rik 4:intergenic:Zfp640-Gm17404 4:intergenic:Gm3944-Gm5784 4:intergenic:Ncald-Rps19-ps5 4:intron:Lrrc7 3:intergenic:Gm24963-Gm10742 4:intron:Steap3 4:intergenic:Gm14516-Cypt1 4:intergenic:Fgd3-Bicd2 4:intergenic:Cdh18-Gm24656 3:intergenic:Gm26535-Gm24710 4:intron:Thsd7b 4:intergenic:Trat1-Retnlb 4:intergenic:Asb9-Gm15216 4:intergenic:Gm7821-Gm7823 4:intergenic:Gm14539-Gm23628 4:intergenic:Rxfp3-Adamts12 4:intron:Rftn1 3:intergenic:Gm14983-Diap2 4:intergenic:Gm15442-Gm24749 4:intergenic:Gm7191-Gm23494 4:exon:Asph/Clvs1 4:intergenic:1700010H22Rik-1700007G11Rik 4:intergenic:Gm12851-Usp1 3:intron:Tenm1 4:intron:Agbl1 3:intron:Fam172a 4:intergenic:Gm22939-Cntnap2 4:intergenic:B230323A14Rik-Bnip2 4:intergenic:2700089E24Rik-Gm23403 4:intron:Tcf7l2 4:intergenic:Agfg1-Slc19a3 4:intron:Slc4a4 3:intergenic:D3Ertd751e-Gm25714 4:intron:Scube3 4:intergenic:Gm25715-N6amt1 4:intron:Ppfibp2 4:intergenic:Gm24294-Fhod3 4:intergenic:Wdr20b-Gm26015 4:intron:4930430A15Rik 3:intron:Col12a1 3:intergenic:Gm9621-Gm24200 4:intergenic:Gm11810-Rab2a 4:intergenic:Gm6207/Gm15020-Gm15020 4:intergenic:Gm15015-Gm15015/Gm6228 4:intron:Spag16 4:intergenic:Slc35g1-Gm24930 4:intergenic:Gadd45g-Gm26651 4:intron:Brinp1 4:intron:Nrip1 3:intergenic:Tmem26-Gm24172 4:intergenic:Gm22444-mmu-mir-6238 4:intergenic:Gm22254-Gm8993 4:intergenic:Gm22538-Bche 4:intron:Pip4k2a 4:intergenic:Nebl-Gm13324 4:intron:Acpp 4:intron:Zpld1 3:intergenic:Gm6851-Gm24910 4:intergenic:Gm4705-Soga2 4:intergenic:Hlx-Marc1 4:intergenic:Foxo1-Gm10293 4:intron:Kcnd2 4:intergenic:Gm7931-Slit2 4:intergenic:Gm12008-Gm12009 4:intergenic:Gm14645-Gm16436 4:intergenic:Zic2-Gm10837 4:intron:Nrxn3 3:intron:Slc24a2 4:intergenic:Gm6767-Cblb 4:intergenic:Gm25550-Cdh9 4:intergenic:Gm13726-4930440I19Rik 4:intergenic:4930474N05Rik-Gm24394 4:intergenic:Gm25267-Il15 4:intron:Ptprm 4:intergenic:Dnah5-Mir3964 4:intergenic:Tenm4-Rps11-ps5 4:intergenic:Gm23708-G6pd2 4:intron:Svep1 3:intron:Park2 4:intergenic:Lrrtm3-Ctnna3 3:intergenic:Gm24350-Gm13464 Off-target primers show all... show less...
408 / fw	`ATTAAATGCCTTCAGAGACC AGG` `.................... ...` Enzymes: StyD4I, LpnPI, CsiI, BstNI Cloning / PCR primers	59	80	58	37	64	80	0 - 0 - 7 - 44 - 161 0 - 0 - 1 - 13 - 10 212 off-targets	4:intron:Rufy3 3:intergenic:Pdzd8-Emx2os 4:intergenic:Gm14566-Gm26437 3:intron:Lama3 4:intron:Plcl2 4:intergenic:Trip4-n-R5s85 4:intergenic:Gm17608-Olfr1402 4:intergenic:Dst-Gm23792 3:intergenic:Gm22727-Gm4258 4:intron:Kctd2 4:intron:Gm595 4:intergenic:Pbp2-Dynlt1-ps1 3:intergenic:Tnp1-Pinc 4:intergenic:4930467D21Rik-Antxr2 4:intergenic:Fam155a-n-R5s95 4:intergenic:Gm15221-E130120K24Rik 4:intergenic:Tacc1-Gm10689 4:intron:Itpr1 4:intergenic:Gm10051-Gm15627 4:intergenic:Olfm4-Gm9578 3:intergenic:Gm22825-Zfhx3 4:intron:Cdk5rap2 4:intron:Srsf11 4:intergenic:Olfr27-Olfr943 4:intron:Tenm2 4:intergenic:Il17a-Il17f 4:intergenic:Tmc1-Zfand5 4:intron:Gpr98 4:intergenic:Itpk1-Gm21971/Itpk1 4:intergenic:Gm7488-Tbl1xr1 4:intergenic:Loxl4-Gm25216 4:intron:St8sia2 4:exon:Ddx5 4:exon:Gm12183 4:intergenic:Tcf7l2-Gm22271 4:intron:Mecom 4:intron:Sptbn4 3:intergenic:Gm12465-Gm12466 4:intergenic:Ppa2-Gm25042 4:intergenic:Fbxo8-Hand2 4:intergenic:Spry2-Gm4775 2:intergenic:Gm12687-Gm12688 4:intergenic:Ttc32-Gm7099 3:intron:Rpgrip1l 2:intron:Kcns3 4:intergenic:Gm16420-Gm5863 4:intergenic:Olfr134-Olfr135 4:intergenic:Gm24297-Gm22911 4:intergenic:Cd160-Cd160/Pdzk1 4:intergenic:Gm22312-Magi1 4:intergenic:Gm23763-Gm25609 4:intergenic:Pdk4-Dync1i1 3:intron:Nav2 4:intergenic:Gm20632/Hist2h2aa2-Gm20633/Hist2h2aa1/Gm20634/Hist2h3c1 3:intergenic:2310081O03Rik-Gm12518 3:intergenic:Gm24382-Mbnl1 4:intron:4930523C07Rik 4:intron:Gm13925 4:intron:Angpt2 4:intergenic:Gm26578-Gm22140 3:intergenic:Taf1-Ogt 4:intergenic:Gm26190-B3gat1 4:intron:Gm14905 4:intergenic:Cxcr5-Ddx6 4:intergenic:Katnbl1-Emc7 4:intron:Csmd1 3:intergenic:Myb-Gm2467 4:intergenic:Gm12154-Gm12155 4:intergenic:Gm831-Gm12962 4:intergenic:Vmn1r-ps150-Zfp677 4:intergenic:Mir690-Mb21d2 4:intron:Rp1l1 4:intergenic:4933436I01Rik-Mir201 4:intergenic:4933436I01Rik-Mir201 4:intergenic:4933436I01Rik-Mir201 4:intergenic:Rpl7a-ps13-4933436I01Rik 4:intergenic:4933436I01Rik-Mir201 4:intergenic:Gm23384-Gm11851 4:intergenic:Rhbdd1-Gm22112 4:intron:Slc17a8 4:intron:Gabrg2 4:intergenic:A930012L18Rik-Mcc 3:intergenic:Trdv4-Trdd1 4:intergenic:Gm24887-Rab11fip4 4:intergenic:Klhl8-Gm24897 4:intron:D230022J07Rik 4:intron:Fyn 4:intergenic:Cacng3-Rbbp6 4:intron:Camta1 3:intergenic:Rpsa-ps10-Mir3963 4:intergenic:Kbtbd12-Gm26588 4:intergenic:Actrt1-Gm14654 4:intergenic:Fzd5-Plekhm3 3:intergenic:Gm23321-Gp2 4:intergenic:Faim2-Gm5808 4:intergenic:Ttll7-Gm23131 3:intron:Dlgap2 3:intron:Mgmt 4:intergenic:Gm12297-Gm12298 4:intergenic:Stpg2-Gm24260 4:intergenic:Spanxn4-Gm7763 4:intergenic:Pdzrn3-Gm9871 4:intron:Tcerg1l 4:intergenic:Safb-2410015M20Rik 4:intron:Nr3c2 4:intergenic:Tmtc4-Nalcn 4:intergenic:Cdh7-Cdh19 4:intergenic:Mapt-Kansl1 4:intron:Mthfd1l 4:intergenic:Gm26076-Gm25519 4:intergenic:Gm13458-Gm13455 4:exon:Nfam1 4:intergenic:Gtf2e2-Rbpms 4:intron:Plb1 3:intron:Arhgap42 4:intron:Mphosph9 4:intron:Ctnna2 4:exon:Tfcp2 4:intron:Tmem45b 4:intergenic:Gm11847-Gm11846 3:intergenic:Gm26811-Plxna1/Gm26811 4:intergenic:Gm22777-Spock1 4:intergenic:Gm25051-Gm1818 3:intergenic:Smlr1-Tmem200a 4:intergenic:Mirlet7a-1-Gm25232 4:intron:8430427H17Rik 3:intergenic:Gm1527-Egfem1 4:intron:Zfand3 4:intergenic:Gm11956-Osm 4:intron:Smpd3 4:intron:Bloc1s5 4:intergenic:Fstl5-Gm23644 4:intergenic:Pcgf5-Hectd2 2:intergenic:Gm14693-Gm14687 4:intron:Grin2b 3:intron:Tanc1 3:intergenic:Gm24238-Dsc3 4:intergenic:Pou5f2-Fam172a 4:intron:Tenm1 4:intergenic:n-R5s30-Rbfox1 3:exon:Slc26a5 3:intergenic:Gm16331-Dach1 4:intron:Dnah9 2:intron:Atp6ap1l 4:intron:Gpc4 3:intergenic:Gm7481-Prkd1 4:intron:Pds5b 4:intron:Lrrc20 4:intron:Gfod2 4:intergenic:Gm13849-1700012A03Rik 4:intergenic:Gm25036-Sema6a 4:intron:Samd4 3:intergenic:Mvk-Gm13790 3:intergenic:AC107236.1-Gm8623 4:intergenic:Gm25144-Gm23147 4:intron:Itga2 4:intron:Iqca 4:intergenic:Cd3d-Cd3e 4:intergenic:Nmbr-Gm23892 4:intron:Nck2 4:intergenic:Hotair/HOTAIR_3-HOTAIR_4 4:intergenic:Pdcd1-Gm23389 4:intergenic:Nav3-E2f7 4:intergenic:Sema3d-Gm23243 4:intergenic:Gm15423-Sdccag8 4:intergenic:Sgcz-Gm23128 2:intron:Kdm4c 3:intergenic:Gm6023-Gm14559 2:intron:2610002M06Rik 4:intron:Grik1 4:intron:Ppp1cc 4:intergenic:RP23-131O4.2-Gm23470 4:intergenic:Klhl2-Gm26210 4:intergenic:Cdh22-Gm25569 4:intron:Pibf1 3:intergenic:Gm12948-Gja4 3:intergenic:Gm23209-Rere 4:intron:Dock3 4:exon:Sec24b 4:intergenic:Mir129-1-Lep 3:intergenic:Gm25339-Gm7275 4:intergenic:Htr1a-Dph3b-ps 4:intron:Wwox 4:intergenic:Gm23644-Gm15442 4:intergenic:Gm25346-Eepd1 4:intergenic:Gm22308-Cbfa2t2-ps1 4:intergenic:Gm5354-Inpp4b 3:intergenic:Kcnj12-Tnfrsf13b 3:intergenic:A230087F16Rik/Cdc42bpb-Cdc42bpb 4:intron:Gm12823 3:intergenic:Gm9423-Depdc1a 3:intron:Tra2a 3:intergenic:2410088K16Rik-Sh3bp4 3:intron:Snx3 3:intergenic:Gm15606-Opcml 4:intergenic:Atp6v1b2-Lzts1 3:intergenic:Gm15618-Colgalt2 4:intron:Pik3ca 2:intergenic:Mrps23-1700106J16Rik 4:intergenic:Tldc1-Cotl1 3:intron:Syt6 3:intergenic:Gm15904-Adc 3:intergenic:RP23-341H6.1-Lca5 4:intergenic:Sp5-1500002O10Rik 3:intergenic:Vps13c-Gm22382 4:intergenic:Gm12020-Gm12022 4:intergenic:Cnr1-Gm22010 4:intergenic:Gm14688-Gm4910 4:intron:Pvrl1 4:intron:Slc5a12 4:intron:Ubtd1 4:intergenic:Gm23816-Serpine3 Off-target primers show all... show less...
136 / rev	`AGCTCAAGTTAGGCCAGTCA TGG` `.................... ...` Enzymes: NlaIII Cloning / PCR primers	58	80	57	49	61	80	0 - 0 - 8 - 28 - 166 0 - 0 - 2 - 1 - 6 202 off-targets	3:intergenic:4930448E22Rik-Gm22659 4:intergenic:Gm15442-Gm24749 4:intron:Zfp536 2:intergenic:Cdh10-Gm25976 2:intergenic:Gm26028-Gm23576 3:intergenic:Parp11-Efcab4b 4:intergenic:Selrc1-Fam159a 3:intergenic:Gm23441-Cypt12 4:intergenic:Fads2-Fads1 4:intergenic:Sntb2-Gm16208 4:intergenic:Pabpc2-Yipf5 4:intergenic:Pde7b-4930403O15Rik 4:intron:Btbd7 4:intron:Erbb4 4:intergenic:Lphn3-Mir1187 4:intergenic:BC040587-Gm26381 4:intron:Slc44a5 4:intergenic:4931417E11Rik-Gm26179 4:intergenic:Pigl-Gm12949 4:intergenic:Myo9b-Use1 4:intron:Stox2 3:intergenic:Gm25985-Gm6404 4:intron:Gm7073 3:intron:Hvcn1 4:intergenic:Hs3st3a1-Gm24275 4:intergenic:Gm11625-Brca1 3:intron:Kcnj16 3:intergenic:Cxcl15-Cxcl1 4:intron:Slc24a3 4:intergenic:Gm23067-Gm26072 4:intergenic:Gm22217-Gm22448 4:intron:Elmo1 4:intergenic:4930564G21Rik-Grip1 4:intergenic:Gm22016-Cdc73 4:intron:Lpo 4:intron:Wdr7 4:intergenic:Mc5r-Mc2r 4:intergenic:Gm5046-Gm23907 4:intergenic:Stip1-Macrod1 4:intergenic:Kdm4c-SNORA43 4:intergenic:Gm25843-Tmeff2 4:intron:Fndc3a 4:intron:Prdm15 4:exon:Fitm2 4:intergenic:Gm16112-Gm16110 4:intron:Chgb 4:intron:Nrg3 4:intron:Dpyd 4:intergenic:Gm25113-Car10 4:intergenic:Gm22600-Gm12040 4:intergenic:Gm26883-Gm26883/Tshz2 4:intron:Gm156 4:intergenic:Lipk-Lipn 4:intergenic:Zg16-Gm22747 4:intergenic:Stpg2-mmu-let-7j 3:intron:Cnnm2 3:intergenic:Olfr948-Olfr951 4:intron:Ubtd1 2:intron:Btbd8 4:intergenic:Gm25650-Gm23584 4:intergenic:F830104G03Rik-Gm22269 2:intergenic:Gm5457-Gm5087 2:intergenic:Brd7-Nkd1 4:exon:Carkd/Cars2 4:intergenic:Cep72-Slc9a3 4:intergenic:Gm7148-Pgk2 3:intron:5730422E09Rik 4:intergenic:Eif3s6-ps1-Gm11995 4:intergenic:D630039A03Rik-Txn1 3:intron:Btbd11 4:intergenic:2310016G11Rik-Izumo2 4:intergenic:4930445B16Rik-Lrrc4c 4:intergenic:Gm26108-Pou3f4 3:intergenic:Gal-Ppp6r3 4:intergenic:Gm24005-Gm26362 4:intergenic:Crisp1-Gm17495 4:intergenic:Gm22429-Gm23659 4:intergenic:Gm22503-Gm24271 4:intergenic:Gm20581-Gm20579 4:intergenic:4933406G16Rik-Gm12025 4:intergenic:Map6d1-Parl 4:intergenic:Ccdc50-Fgf12 3:intergenic:AC102758.1-Sox6 4:intergenic:Gm10543-Gm25578 4:intergenic:Gm22536-Gm6632 2:intergenic:Pou3f4-Cylc1 3:intergenic:Gm10620-Cmc2 3:intergenic:Gm15168-Gm5764 3:intergenic:Plod2-Gm9621 3:intergenic:1700025D23Rik-1700001J04Rik 4:intron:Pias4 4:intron:Ubn2 4:intergenic:4933417D19Rik-4933417D19Rik/Vps9d1 3:intergenic:Eepd1-Gm26190 4:intergenic:BC048507-Mtrr 4:intergenic:BC048507-Mtrr 4:intergenic:Pde7b-4930403O15Rik 3:intergenic:Gm12160-Gm12161 4:intron:Tmem53 4:intergenic:Lpp/1110054M08Rik-Gm24440 4:intergenic:Tnfrsf13c-Cenpm 4:intergenic:Tnfrsf13c-Cenpm 4:intergenic:RP24-338F2.1-Tac1 4:intron:Acad11 4:intergenic:Gm22565-Gm24288 4:intergenic:Gm23594-Il20rb 4:intergenic:4932415D10Rik-Gm4924 4:intergenic:Gm23000-Gm8310 4:intron:Park2 4:intergenic:Gzmk-Esm1 4:exon:Gm3550 3:intergenic:4930448N21Rik-Trappc11 3:intron:Tdp1 4:intron:Plcl2 4:intergenic:Gm8312-1700036O09Rik 4:intergenic:Gm5937-Tbl1x 4:intron:BC048562 4:intergenic:Tmem132d-Gm17132 4:intergenic:Gm24393-RP23-452E7.2 3:intron:C630028M04Rik 3:intergenic:Gm14832-Eda 4:intron:Reep3 4:intergenic:Zdhhc14-3300005D01Rik 4:intron:Hal 3:intergenic:Gm11919-Gm11923 4:intergenic:4930578M01Rik-Gm8973 4:intergenic:Gm11189-Hspa4 4:intron:Tmem33 4:intergenic:1700066O22Rik/4930511M06Rik-1700066O22Rik 4:intron:Rnf4 4:intron:Stau2 4:intergenic:Kdm4c-SNORA43 4:intron:Utrn 4:intergenic:Gm25255-Csmd1 4:intron:Sirt2 4:intergenic:A430057M04Rik-Zfp503 4:intergenic:Gm6771-Ptchd4 4:intergenic:Slc2a3-Gm26826 4:intron:Add1 4:intron:Gtf2h3 3:intron:Dab2ip 4:intergenic:A730085K08Rik-9530059O14Rik 4:intergenic:Gm22011-Mthfr-ps1 2:intergenic:Gm12080-Gm6685 4:intergenic:Gm14885-BC049702 4:intergenic:Gm23822-4921504A21Rik 4:exon:ACE3/Ace3 4:intergenic:St6gal1-Gm23430 4:intergenic:Col15a1-Tgfbr1 4:intergenic:4933402E13Rik-Gm14679 4:intron:Tac1 4:intergenic:Gm23039-Sgcd 4:intergenic:Gm25550-Cdh9 4:intergenic:Sgcd-Gm12178 4:intron:Nav2 4:intron:Cdk14 4:intergenic:Gm8941-Gm24402 4:intergenic:Ncam2-Gm23355 4:intron:Gpc5 4:intergenic:Peli2-Gm23881 4:intergenic:Steap4-Gm15731 4:intergenic:Stard4-Nrep 4:intergenic:Wbscr17-Auts2 4:intron:Morc1 4:intergenic:Zfp652-Phospho1 4:intron:Igf1r 3:intergenic:Gm22845-Nt5c1b 4:intergenic:Gm2663-2210010C04Rik 4:intron:Ehbp1 4:intron:Lrrfip2 4:exon:Sv2c 2:intron:Camk1d 4:intergenic:Usp7/Gm24814-Usp7 4:intron:Ttc29 4:intron:Mpp7 4:intergenic:1700008P02Rik-Gm22074 3:intron:Fut8 4:intergenic:Gm24691-Grm8 3:intergenic:Gm8325-P2ry1 4:intergenic:Stag3-Gm10874/Got2-ps1 4:intergenic:Gm14505-Gm9435 4:intergenic:Gm6071-Gm25812 4:intergenic:Gm13714-Olfr987 4:intergenic:Samt3-Nr0b1 4:intron:Pcdh9 4:intergenic:Gm11042-Kcnh5 4:intergenic:Gjb5-Gm12947 4:intergenic:Gm24246-Pik3c3 4:intergenic:Pja1-Gm14816 4:intron:Ccdc50 4:intergenic:Gm12295-Gm26128 4:intergenic:1700007B14Rik-Gm7901 4:intergenic:Tnks/Gm9939-Rpl31-ps23 4:intergenic:Pof1b-Gm14922/Gm14923 4:intergenic:Slitrk2-Gm23000 4:intergenic:Gm11810-Rab2a 4:intron:Nfatc1 4:intron:Ap2a2 4:intergenic:Elovl2-Smim13 4:intron:Slc13a2 4:intergenic:Ighg1-Ighg3 4:intergenic:Gm11680-BC006965 Off-target primers show all... show less...
90 / rev	`CCAGTTTAATTCTGAGTTTA AGG` `.................... ...` Inefficient Enzymes: BshFI, Tru1I, LpnPI, BstC8I Cloning / PCR primers	55	74	19	33	67	80	0 - 0 - 2 - 45 - 225 0 - 0 - 0 - 5 - 7 272 off-targets	4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Mylk-Ptplb 4:intergenic:Gm23153-Rps6kc1 3:intergenic:Sptlc2-Alkbh1 4:intergenic:Gm6594-Pkdcc 4:intergenic:B230110C06Rik-Oxsm 4:intergenic:Gm24957-Gm22492 4:intergenic:Gm15443-Gm23535 4:intergenic:Gm6600-Gm8837 4:intergenic:Gm27047-Gm26919 4:intergenic:Gm3839-Ptprg 4:intergenic:Clca6/Gm16233-Gm16233/Clca6 4:intron:Mc2r 4:intergenic:Gm13254-Gm24534 4:intergenic:Gm5084-Gm24999 4:intergenic:Gm24836-Sde2 3:intergenic:Sqrdl-Gm13989 4:intron:Ago2 3:intron:Zdhhc20 3:intron:Fry 4:intergenic:Gm14977-Gm4994 4:intergenic:Mogat2-Gm26705 4:intergenic:Gm14765-Tab3 4:intergenic:Gm4784-Gm4991 4:intron:Dock7 4:intergenic:Gm7099-Osr1 4:intergenic:Phyhipl-4930533K18Rik 4:intron:Tns3 4:intron:Kmt2a 4:intron:Gucy1a3 4:intron:Sybu 4:intergenic:Prickle1-Adamts20 4:intron:Kpnb1 4:intergenic:Gm8210-Nck2 4:intron:Lrr1 4:intergenic:Gm24009-Mir3096 4:intron:Kcnq3 4:exon:Gm20707 4:intron:Atg7 4:intergenic:Kcnk13-Psmc1 3:intron:Brinp2 3:intergenic:Dsc3-Dsc2 4:intergenic:Gm7816-Gm2810 4:intergenic:Abcc3-Cacna1g 4:intergenic:Cntn5-Gm25365 4:intergenic:Gm23459-Gm24634 4:intergenic:Gm26354-Gm7536 4:intergenic:Gm12344-Efcab5 4:intergenic:Cdh11-Gm8730 4:exon:Fam19a3 4:intron:Pibf1 4:intergenic:Gm22016-Cdc73 4:intergenic:F630040K05Rik-Vegfa 4:intergenic:Gm22492-Pdcd6ip 4:intron:Tmem117 4:exon:Gm13490 4:intergenic:Gm21232-Gm27044 4:intergenic:Agpat9-Gm20484 4:intergenic:Gm23186-Arid1b 4:intergenic:Sox6-1700003G18Rik 4:intron:Unc13b 4:intergenic:Trim27-Gpx5 4:intergenic:Gm9843-Usp25 3:intergenic:Gm24104-Gm26058 4:intergenic:1700065I17Rik-Lmnb1 4:intergenic:Gm21045-Gm5576 4:intergenic:Gm14148-Gm14152 4:intergenic:Gm4791-Gm22286 4:intergenic:4930583I09Rik-Gm25344 4:intergenic:Shisa9-Gm6327 3:intergenic:Gm25602-Tbc1d32 4:intergenic:Gm15711-Gm9968 4:intergenic:C130026L21Rik-Gm26897 4:intergenic:Mir760-Bcar3 4:intron:Kansl1 4:intron:Adam12 4:intergenic:Btg2-Gm8618 4:intergenic:Lcorl-Gm7931 4:intergenic:Rps2-ps6-Tsc22d1 4:intergenic:Tpx2-Gm24421 4:intron:Adam17 4:intergenic:Cdh9-Gm22032 4:intergenic:Dmrt2-Gm8825 4:intergenic:Hist1h2be-Hist1h2be/Gm11398 4:intron:Usp24 4:intergenic:Trim63-Gm13195 4:intergenic:Gm21232-Gm27044 4:intron:Slc9a9 4:intergenic:4931417E11Rik-Gm26179 4:intergenic:Gm25935-Gm22593 4:intergenic:Gm22673-Gm23059 4:intergenic:4930557A04Rik-Sytl5 4:intergenic:Trps1-Eif3h 4:intron:Lin7c 4:intergenic:Mib1-1010001N08Rik 4:intergenic:Mtpap-9430020K01Rik 3:intergenic:Gm22544-Gm22509 4:intergenic:Gm11747-Gm11748/Engase 4:intron:Bax 4:intergenic:Gm22939-Cntnap2 3:intergenic:Gm15074-Gm4996 3:intron:Sv2b 4:intron:Gatad2b 4:intron:Epha6 4:intergenic:Vmn2r98-Vmn2r-ps119 4:intergenic:Cmtm2a-Cmtm1/Cmtm2a 4:intergenic:Gm26901-Sntg1 3:intergenic:Slc4a4-Gc 4:intergenic:Fam168a-Gm3200 3:intron:Atp8b4 4:intron:Ccser1 4:intergenic:Rpl41-Pa2g4 4:intergenic:Gm15498-Gm15753 4:intron:Plxdc2 4:intergenic:1600025M17Rik-Zfp449 3:intergenic:Fshr-Gm10184 4:intergenic:Gm24963-Gm10742 4:intron:Rbl1 4:intergenic:Gm23459-Gm24634 4:intergenic:B430212C06Rik-B430212C06Rik/Cidea 4:intergenic:RP24-108J19.1-Lrrc6 4:intron:BC023829 3:intergenic:Gpr6-Fig4 4:intergenic:Csnka2ip-4930453N24Rik 4:intron:Sycp2 4:intron:Chrna3 4:intron:Gpr137b 3:intergenic:Gm5513-Gm24319 4:intron:Ttc23l 4:intron:Grik5 4:intergenic:Tpk1-Gm22939 3:intergenic:Gm23644-Gm15442 4:intron:Coq10b 4:intergenic:Olfr826-Olfr827 4:intergenic:Gm5117-Nrg1 4:intergenic:2500002B13Rik-Hmgb2 3:intergenic:Gm25935-Gm22593 3:intergenic:4933434M16Rik-2610035D17Rik 4:intron:Sox5 4:intron:Sfxn5 4:intergenic:Fam19a1-Fam19a4 4:intron:Tbc1d19 4:intergenic:Phlpp1-Bcl2/D630008O14Rik 4:intron:Lancl3 4:intergenic:Gm26399-Gm5913 4:intron:Rbpms 4:intergenic:Tenm2-Gm12128 4:intergenic:Mir465-Gm14683 4:intergenic:Mir3963-Eltd1 4:exon:Mga 4:intergenic:Gm23511-Gm22480 4:intergenic:Ar-Gm14811 4:intergenic:Fam32a-Ap1m1 4:intergenic:Dbx1-Htatip2 4:intron:Phka1 4:intergenic:1700017N19Rik-Gm26278 3:intron:Spag16 4:intergenic:Gm15369-Gm15374 3:intron:Ext1 4:intergenic:Card11-Gm26113 4:intergenic:Irf9-Rec8 4:intron:Caln1 4:intron:Mei4 4:intergenic:Rps10-ps2-Gm9697 4:intergenic:Fmod-Btg2 4:intergenic:Ctbp2-Tex36 3:intergenic:n-R5s30-Rbfox1 4:intergenic:Gm14624-Gm22103 4:intergenic:PVT1_3-Ndufa4 4:intergenic:Gm4784-Gm4991 4:intergenic:Bche-Gm6098 4:intron:Emcn 4:exon:Gm13437 4:intergenic:Gm9697-2310042E22Rik 4:intron:Sucla2 3:intron:B4galnt2 4:intron:Vrk3 4:intron:Pecam1 4:intron:Lnp 4:intergenic:Gm25591-Syt1 4:intergenic:Gm26235-Gm25392 3:intergenic:Gm26052-Slc39a11 4:exon:Neat1/NEAT1_3/NEAT1_2/NEAT1_1 3:intergenic:Gm22186-Gm22236 3:intron:Gabpa 4:intergenic:Icos-Rpl17-ps1 4:intergenic:Plxdc2-Gm24933 4:exon:Trak2 4:intron:Prkch 3:intron:Fndc3c1 4:intergenic:Ctnna3-Gm22829 4:intergenic:Ticam2-Tmed7 4:intergenic:Gm15416-Tenm4 4:intron:Wbscr17 4:intergenic:Gm4487-Gm24073 4:intergenic:Gm22508-Ccdc68 4:intron:Stk39 4:intergenic:Gm10136-Cdc37l1 3:intergenic:Gm24591-Gm26241 4:intergenic:Fbxo15-Gm25453 3:intergenic:Gm22104-Gm25550 4:intergenic:Dnah8-Glp1r 4:intron:Papola 4:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:Gm23986-Gm24929 4:intron:Plxna2 4:intergenic:Gm14270-Bcas1 4:intergenic:RP24-376I11.1-RP23-402M18.1 4:intergenic:Gm11816-Clvs1 3:intergenic:Gm13217-1700061F12Rik 4:intergenic:Gm23558-Galc 3:intergenic:E430024P14Rik/Tagap-Rsph3a/E430024P14Rik 2:intron:Rhobtb1 4:intergenic:Ino80c-Galnt1 4:intron:Tmcc1 4:intergenic:Gm11676-Gm11677 2:intron:Zfp91 4:intergenic:Gm26291-Gm17377 4:exon:Zfp94 3:intergenic:Atg10-A830009L08Rik 3:intergenic:Pinc-Gm25329 4:intergenic:Hs1bp3-Rhob 3:intergenic:Gm26182-Gm14929 4:intron:Fam49b 3:intergenic:Hibadh-Gm24355 4:intergenic:Barx1-Phf2 3:intergenic:Gm5943-Gm14905 4:intron:Kif21a 4:intron:Lrp1b 4:intron:Csmd1 4:intron:Bag4 4:intergenic:Gm8214-Dusp10 4:intergenic:Mast4-Gm5454 4:intergenic:Spata16-Ect2 4:intergenic:4930405D11Rik-Kif2b 4:intron:Hpse2 3:intergenic:Wwox-Gm23677 3:exon:Metrn/Gm26694 4:intergenic:Ahctf1/Gm10518-Cdc42bpa 4:intergenic:Olfr1344/Gm20715-Olfr1336 3:intergenic:Gm5507-Redrum 4:intergenic:Gm14914-Gm14917 3:intergenic:Rgs3-Gm11209 3:intergenic:Tctn1-Pptc7 3:intergenic:Gm6024-6030498E09Rik 3:intergenic:Gm26580/Kcnq5-Rims1 3:intergenic:Gm13255-Gm13217 3:intergenic:Chmp2b-Vgll3 4:intergenic:Sgcz-Gm23128 4:intron:Cyp2c38 4:intergenic:Maob-Ndp 4:intergenic:Gm24767-Gm17733 4:intron:St18 4:intron:Zfml 4:exon:Rab11fip1/4933416M07Rik 4:intergenic:Gm22183-mmu-mir-378d 4:intergenic:Maob-Ndp 4:intergenic:Rad51b-Zfp36l1/Gm26669 4:intergenic:Lrp1b-mmu-mir-6336 4:intergenic:E230019M04Rik-Gm15046 4:intron:Hmgxb4 4:intergenic:Gm12370-Mir876 4:intron:A230006K03Rik 4:intron:Gm15908 4:intron:Prkaa2 4:intergenic:Clstn2-4921534H16Rik 4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Gm25442-Gm25696 4:intergenic:Rpl10-ps3-1600029D21Rik 4:intron:Acsl5 4:intron:Sspo 4:intergenic:Gm22891-Gm24774 Off-target primers show all... show less...
431 / fw	`TAATAAACTAAAGACTGGGC AGG` `.................... ...` Enzymes: BmuI, Bse1I Cloning / PCR primers	54	69	47	24	70	79	0 - 0 - 2 - 30 - 299 0 - 0 - 1 - 6 - 6 331 off-targets	4:intron:Phf3 4:intergenic:9030612E09Rik-Pdss2 4:intergenic:Hk2-Sema4f 3:intergenic:Ybx3-Gm6619/5430401F13Rik 4:intergenic:1700009N14Rik-Gm12385 4:intron:Cnpy1 4:intergenic:Gm14103-Gm14094 4:intron:Itih2 4:intergenic:4933433F19Rik-Dusp26 4:intron:Ube2r2 4:intergenic:Prdm8/A730035I17Rik-Fgf5 4:intergenic:Gm23718-Gm14585 4:intergenic:Igkv4-78-Igkv4-74 4:intron:Tnfrsf21 4:intron:Sema6d 4:intron:Mcmdc2 4:intron:Lclat1 4:intergenic:Vmn2r-ps86-Vmn2r76 4:intergenic:Amer2-Spata13 4:intron:F630040K05Rik 4:intergenic:Cdk15-Fzd7 4:intergenic:Pcdh9-Gm23324 3:intron:Btbd7 4:intergenic:Zfp385b-Gm13661 4:intergenic:Ppp1r2-ps9-Gm14519 3:intergenic:Gm12700-Cachd1 4:intergenic:Klhl3-Hnrnpa0 4:intron:2210408F21Rik 4:intergenic:Extl2-Gm26344 4:intergenic:Crh-4632415L05Rik 3:intergenic:Zg16-Gm22747 4:intergenic:Grpr-Rnf138rt1 4:intergenic:Gm23186-Arid1b 4:intergenic:Bcl3-Gm19345/Ceacam16 4:intergenic:Ntng1-Gm25681 4:intergenic:Tmpo-Rmst 2:intron:Gm11542 4:intergenic:Fndc3a-Cysltr2 4:intron:Med13l 4:intron:Xpr1 4:intergenic:Gm5506-Gm5839 4:intergenic:4933403O08Rik-Gm5393 4:exon:4632427E13Rik 4:intergenic:Gm23838-Gm25233 4:intron:Abcb8 4:intergenic:mmu-mir-6344-Irs1/Gm9747 4:exon:Irak1bp1 4:exon:Gm14542 4:intergenic:Defb11-Defb15 4:intergenic:Lgals4-Lgals7 4:exon:Slc23a1 4:intergenic:Gm23649-Lclat1 4:intergenic:Gm25969-A930011G23Rik 4:intergenic:Bche-Gm6098 4:intron:Clstn1 4:intergenic:Gm4419-Gm5633 4:intergenic:4930593A02Rik-Clrn1 3:intergenic:Pdcl3-mmu-mir-6349 4:intergenic:4930401G09Rik-Gm13832 4:intron:Bbip1 4:intron:Nkain3 4:intron:Zfand3 3:intron:Lin9 4:intron:Scaf4 4:intron:Gm15800 4:intron:Mrpl13 4:intergenic:Ipmk-Gm22937 4:intron:Xpo4 4:intergenic:5430425J12Rik-Slc12a7 4:intergenic:Ptges-Gm13400 4:intergenic:Anxa6-Ccdc69 4:intergenic:Igkv4-61-Igkv4-59 4:intergenic:Igkv4-70-Igkv4-69 4:intergenic:Igkv4-57-Igkv4-56 4:intergenic:Igkv4-71-Igkv4-70 4:intergenic:Rprl1-Igkv4-63 4:intergenic:Gm13337-Myo3a 4:intergenic:Mybpc1-Spic 4:intron:Cntn1 3:intron:Rsrc2 4:intergenic:Gm26396-Gm13795 4:intron:Tbc1d32 4:intron:Pign 4:intergenic:Rnf144b-4931429P17Rik 4:intron:Elmsan1 4:intergenic:Gm12509-Gm12508 4:intergenic:Prps1l3-Gm11025 4:intron:Spata21 4:intergenic:Ptger4-AC133283.1 3:intergenic:Rcn1-4930527A07Rik 3:intron:Dlgap1 4:intron:Itgal 3:intron:Galnt2 4:intergenic:Pik3c2b-Ppp1r15b/Gm26616 4:intergenic:Gm23462-Dennd5b 4:intergenic:Gm24611-Zyx 4:intergenic:Gm10398-Gm26660 4:intron:Phldb3 4:intergenic:Gm24751-Angpt1 4:exon:Kdm2b 4:intron:Bpifc 4:intron:Prl 4:intergenic:Gm15201-Gm23786 4:intron:Cox7b2 4:intergenic:Gm26354-Gm7536 4:intergenic:n-R5s193-Spsb1 4:intergenic:Camp-Cdc25a 4:intergenic:Olfr461-Olfr460 3:intergenic:Emx2-Gm17203 4:intron:Fer1l6 4:exon:Lrrc8e 4:intron:Wdr96 4:intron:Rb1 4:intron:Zscan5b 4:exon:Plekhb2 4:intron:Fcgrt 4:intron:Snx29 4:intergenic:Fndc3c1-Cysltr1 4:intergenic:Gm22782-Rpl10l 4:intergenic:Gm22533-Dnajc6 4:intron:Enah 4:intergenic:Gm23240-Gm8357 3:intergenic:E530001K10Rik-Ttc17 4:intron:Ptprc 4:intergenic:Gm25792-Gm23453 4:intergenic:Gm11244-Gm11245 4:intron:Helq 4:intron:Inadl 4:intergenic:Eef1e1-Gm22035 4:intron:Swap70 4:intergenic:Gm24304-Gm22974 4:intergenic:Grm8-Grm8/Mir592 4:intron:Parvg 4:intergenic:Ndufaf6-Gm11827 4:intergenic:Dmxl1-Tnfaip8 4:intron:Fa2h 4:intergenic:Nol6-Gm12402 4:intron:Adam11 4:intron:Ccbl2 4:intron:Ntm 4:intron:Sgk1 4:intergenic:Gm23038-4930570G19Rik 4:intergenic:Gm20648-Senp6 4:intron:Ppfibp2 4:intergenic:D7Ertd443e-Dock1 4:intergenic:Snx22-Snx1 4:intergenic:Cnbp-Copg1 4:intergenic:Gm23243-Gm10481 3:intergenic:Gm8604-MALAT1 4:intron:Crebrf 4:intergenic:Gm14580-Gm14628 4:intron:Pbx4 4:intergenic:Tcerg1l-Mapk1ip1 4:intergenic:Olfr721-ps1-Slc4a7 4:intron:Cep170 4:intron:Fam117b 4:intergenic:Ube2e3-Gm14466 4:intergenic:Wfikkn2-Gm21885 4:intergenic:1700087M22Rik-Sfmbt1 4:intergenic:1500016L03Rik-1700109G15Rik 4:intergenic:Gm20686-Zfhx3 4:intron:3632451O06Rik 3:intergenic:Akr1c21-Akr1e1 4:intergenic:Lrrc4c-4930445B16Rik 4:intron:Baiap2 4:intergenic:Cdh20-Rnf152 4:intron:Cadps 4:intergenic:Gria3-Gria3/Gm22413 4:intron:Gm11639 4:intergenic:Morc1-Trat1 4:intergenic:Gm15037-Gm14719 4:intron:Slc16a2 4:intron:Slc4a4 4:intron:Baz2b 4:intergenic:n-R5s175-Gm2301 4:intergenic:Klhl9/Gm26566-Gm13274 4:intergenic:Zfp873-AU041133 4:intergenic:Vps25/Ramp2-Vps25 4:intergenic:Gm24601-Gm26501 4:intron:Ttll11 4:intergenic:Atp5g3-Gm13668 4:intergenic:Gm24235-Gm25513 4:intergenic:Mir466j-Gm20611 4:intergenic:Tnfsf15-Tnfsf8 4:intron:Farsb 4:intergenic:Gm11672-Map3k3 4:intergenic:Gm23666-U7 4:intergenic:Mir466f-4-Irx1 4:intergenic:Exoc4-Gm13854 4:intergenic:Map3k9-Pcnx 4:intron:Ccser1 4:intron:Zfpm2 4:intron:Vcp 4:intergenic:Ptprg-3830406C13Rik 4:intergenic:2410066E13Rik-Znrf2 4:intron:Abca12 4:intergenic:Serpinb7-Serpinb2 4:intergenic:Nlrp12-Myadm 4:intergenic:Gm11197-Fam58b 3:intergenic:Gm26535-Gm24710 4:intron:Ino80 4:intergenic:Gm12479-Zfp462 4:intron:Ezh1 4:intergenic:Gck-Ykt6 3:intergenic:Ehbp1-Rpsa-ps3 4:intergenic:Ctnna3-Gm22829 4:intergenic:Agrp-Fam65a 4:intergenic:Gm22796-Lmod1 4:intron:Abi3bp 4:intergenic:Rnpc3-Col11a1 4:intergenic:Gm13983-Bmf 4:intergenic:Tle4-Gm26026 4:intron:Cnot6l 4:intergenic:Mir760-Bcar3 4:intergenic:Ptma-Pde6d 4:intergenic:Aqp11-Pak1 4:intergenic:St3gal6-Gm813 4:intergenic:Gm13653-Gm13659 4:intergenic:Gm24288-Gm17215 4:intron:Atrnl1 4:intergenic:Slc2a10-Eya2 4:exon:Gm20522/Gpank1 4:intergenic:Ntn4-Gm8580 4:intergenic:Slc38a4-Amigo2 3:intron:Stim1 4:intron:Pitpnc1 4:intron:Tacc3 3:intergenic:Bet1-RP23-447L9.1 3:intron:Vps13c 4:exon:Grin3a 4:intergenic:Gpr142-Gprc5c 4:intron:Nebl 4:intergenic:Qrfpr-Anxa5 4:intergenic:Magi1-Slc25a26 3:intergenic:Lipk-Lipn 4:intergenic:Fam155a-n-R5s95 4:intron:Prrg1 4:intergenic:Gm14624-Gm22103 4:intergenic:Gm13583/Pla2r1-Gm13566 4:intergenic:Gm23932-Gm5867 4:intron:Sh3tc2 4:intron:Kcnma1 4:intergenic:Gm4141-Vmn1r107 4:intergenic:Gm6882-Vmn1r130 4:intergenic:Gm10662-Vmn1r165 4:intergenic:Gm5891-Vmn1r137 4:intergenic:Gm4201-Vmn1r155 4:intron:Ankhd1 4:intron:Ttc21a 4:intron:Lpcat3 4:intron:Tenm2 4:intergenic:Amd1-4930520K10Rik/Cdk19 4:intron:Sar1b 4:intergenic:St6galnac2-Gm11735 4:intergenic:Aldh3a2-Slc47a2 4:intron:Tonsl 4:intron:AI597468 4:intergenic:Gm5973-Gm9915 4:intergenic:Six2-Srbd1 3:intergenic:Gm24471-Cartpt 4:intergenic:Rbl1-Gm14277 4:intergenic:Zfp810-Gm10182 4:intron:Spert 4:intergenic:Gm13976-3110099E03Rik 4:exon:Tmem63a 4:intron:Ufsp2 4:intron:Sorl1 4:intron:Coq10b 4:intergenic:Gm25658-Atp2b1 4:intergenic:Gm15245-Mid1 4:intron:Acbd6 4:intron:Ss18 4:intergenic:Gm26925-Lmtk2 4:intergenic:Gm25144-Gm23147 4:intron:Stard13 4:intron:Mthfd1l 4:intron:Apip 4:intergenic:5S_rRNA-Auts2 4:intergenic:6030452D12Rik-Cdh3 4:intron:Magi2 4:intron:Pibf1 4:intergenic:Wdr20b-Gm26015 4:intergenic:AL844604.1-Mpdz 3:intergenic:Lce1m-Gm23784 4:intergenic:Slc22a22-Zhx2 3:intergenic:Gm13773-Gm12567 3:intergenic:Vmn2r-ps119-Vmn2r-ps120 3:intergenic:Mup-ps8-Gm21286 4:intron:Eomes 3:intron:Serinc5 4:intron:Zc3h8 4:intergenic:Lrrc7-Gm9423 4:intergenic:Gm23815-Gm7627 3:intergenic:Gm382-Gm22139 3:intron:Zfml 2:intergenic:Kif14-Gm26598 4:intron:Nipbl 4:intergenic:Gm2245-Fyb/Gm2245 4:exon:Myo18a 4:intergenic:Fam198b-Gm6706 4:intergenic:Gabra6-Gabrb2 4:intron:Shroom3 4:intergenic:Gm13379-Gpr158 4:intron:Fam60a 4:intergenic:Dock1-Nps 4:intron:Phip 4:intron:4930402H24Rik 4:intron:Msh5 4:intron:Stk32b 3:intergenic:Fggy-9530080O11Rik 4:intergenic:4930524J08Rik-Hnrnpdl 4:intergenic:Adamts3-Gm25320 4:intergenic:F830104G03Rik-Gm22269 4:intron:Abcf1 4:intergenic:Gm4799-Chst11 4:intron:Rbfox3 4:intergenic:Mir5099-Meox2 4:intron:Nlrp5 4:intron:Abcf1 3:intergenic:Gm23447-Gm23773 4:intron:Gm14697 4:intergenic:Amer2-Spata13 4:intron:Fstl4 4:intergenic:Pacsin2-Ttll1 4:intergenic:Olfr718-ps1-Rbak 4:intergenic:Usp7-1810013L24Rik 4:intergenic:Gm25497-Lrrn3 4:intergenic:Grin3a-Ppp3r2 4:intergenic:Dcc-Gm25509 4:intron:Cdca7l 4:intron:Bend6 Off-target primers show all... show less...
313 / rev	`ATAAGCAATTACTATTCTTT GGG` `.................... ...` Inefficient Low GC content Cloning / PCR primers	53	71	27	25	61	74	0 - 0 - 3 - 51 - 293 0 - 0 - 0 - 7 - 8 347 off-targets	4:intergenic:Gm26354-Gm7536 4:intron:Abi2 4:intergenic:Cntn6-Gm23339 4:intergenic:Gm5432-Lpin1 4:intron:Zdhhc14 4:intron:Lca5l 4:intron:Sdk1 3:intergenic:Cdh6-Gm9630 4:intron:Slc8a1 4:intergenic:Wdr11-Gm23847 4:intergenic:Gm25495-Gm23225 4:intergenic:Trpm3-Gm22506 4:intergenic:Vmn2r79-Nox4 4:intergenic:Gm24070-Gm24994 4:intergenic:Gm24189-Ncoa7 4:intergenic:Gm26131-Klhl13 4:intergenic:Gm25756-Gm22553 4:intergenic:3100003L05Rik-Gm21957 4:intergenic:Popdc3-Bves 4:intergenic:Shroom3-Gm20500 4:intron:1110037F02Rik 4:intergenic:Gm15663-Mettl25 4:intron:Exoc6 4:intron:Itgb6 4:intergenic:Gm22618-Gm22976 4:intron:2810007J24Rik 4:intergenic:Gm17054-n-R5s37 4:intron:Slc46a3 3:intergenic:Pde4d-Rab3c 3:exon:Qrsl1 3:intergenic:Gm22780-Gm24132 4:intron:2810055G20Rik 3:intergenic:Hdhd1a-Prr16 4:intron:Tmem202 3:intergenic:Gm26124-Gm16391 4:intergenic:Gm15172-Phex 4:intergenic:Fam19a1-Fam19a4 4:intergenic:Gm11214-Tnfsf15 4:exon:C1d 4:intergenic:Gm22331-Gm24937 4:intergenic:4631405K08Rik-Plxna2 3:intergenic:Irx2-Irx4 4:intergenic:Gm23209-Rere 4:intron:Vmn2r75 4:intron:Vmn2r-ps69 4:intergenic:Hist1h4j-Hist1h4k 4:intergenic:Gm22011-Mthfr-ps1 4:intergenic:Cars2-Ing1 4:intergenic:Gm11259-Gm11256 4:exon:Zfp280d 4:intergenic:Gm4950-Gm22259 4:intergenic:Gm16330-Npy5r 4:intergenic:Isl1-Gm23198 4:intergenic:Gramd1c-Atp6v1a 4:intergenic:Kcnc2-Atxn7l3b/Gm26596 4:intron:Cd244 4:intergenic:Gm25133-Gm24770 4:intron:Angpt2 4:intron:Ccdc92 4:intron:Klhl14 3:intergenic:Gm21596-Gjd2 4:intergenic:Rpl28-ps4-Gm9946 4:intergenic:Apol7d-Gm5528 4:intron:Kdm3b 4:intergenic:Kcnt2-Gm4845 4:intron:Strn3 4:intron:Scfd1 4:intergenic:Gm24591-Gm26241 4:intergenic:Gm23041-Nav3 4:intergenic:Gm11890-Gm11892 4:intergenic:Maf/Gm15655-Dynlrb2 4:intron:Brwd1 4:intergenic:Gm11534/B4galnt2-B4galnt2 4:intergenic:Gm12868-Zfp691 4:exon:Akap8 4:intergenic:Gm22223-Gm22715 4:intron:Dnm1 4:intergenic:AC154801.1-BC040587 4:intron:Pigv 4:intron:Tmem236 4:intergenic:Gm22628-5033411D12Rik 4:intergenic:Gm9672-Gm17208 4:intergenic:Gm6031-Ube2v2 4:exon:Epha4 4:intergenic:Gm13916-Gm13915 4:intron:Lmo3 3:intergenic:Gm15943/Klhl38-Anxa13 4:intergenic:Gm26137-Gm23733 4:intron:Dkk2 4:intergenic:Gm26235-Gm25392 4:intron:Pdlim3 4:intron:Magi1 3:intron:5033411D12Rik 4:intergenic:Gm24438-Gm16373 4:intergenic:4930467D21Rik-Antxr2 4:intergenic:Gm7722-Gm14609 4:intron:Nrg4 4:intergenic:Gm26913-Gm4076 4:intron:Tes 4:intron:Abca13 4:intergenic:Gm1815-Zfp353-ps 4:intergenic:Vnn1-Taar1 3:intron:Dlg2 3:intergenic:Gm5124/Gm26593-Agtr2 4:exon:Dimt1/Kif2a 4:intergenic:Plk2-Actbl2 4:intergenic:Gm14977-Gm4994 4:intron:Gm13112 4:intron:4930529M08Rik 4:intron:Capn6 4:intergenic:Gm15985-Gm6116 4:intron:Edil3 4:intergenic:Rpl36-ps3-Mycn 4:intergenic:Gm22273-Gm24079 4:intergenic:Amd2-Hs3st5 4:intron:Bmpr1b 4:intergenic:Gm25269-Ush2a 3:intron:Wt1 4:intron:Oacyl 4:intergenic:Nsun3-Arl13b 4:intergenic:Gm23315-Rprm 4:intron:Kcnj3 3:intergenic:Gm23180-Gm22977 4:intron:Pitpnb 4:intergenic:Nudcd3-Rps15a-ps6 4:intergenic:Cdr1-Gm14665 4:intergenic:Tdrd3-Rps3a2 4:intron:Pum2 4:intergenic:Gm25669-Cep63 4:intergenic:Gm9843-Usp25 3:intron:Mef2c 4:intergenic:Sergef-Gm15700 3:intergenic:Gm815-Vldlr 4:intergenic:Gm26119-Fzd8 3:intron:Utp6 4:intron:Slc38a10 4:intergenic:Gm24213-mmu-mir-6370 4:intron:Dram1 4:intergenic:Gm25183-Spats2 4:exon:Prrg1 4:intron:Clstn2 4:intergenic:Gm8226-Gm26126 4:intergenic:Olfr1035-ps1-Olfr1036 4:intergenic:Gm11986-U7 4:intron:Ogn 4:intergenic:Gm7952-Slco6d1 4:intergenic:Gm23752-Gm24815 4:intergenic:Gm24349-1700019A02Rik 4:intergenic:Gm26190-B3gat1 4:intron:Corin 4:intergenic:Gm12017-Gm12018 4:intergenic:Gm7276/St8sia5-St8sia5 4:intron:Aff1 4:intergenic:Zc3hav1-Rpl30-ps1 4:exon:Gm16185 4:intron:Lgmn 4:intron:Gnal 3:intergenic:Gm26206-Xkr4 4:intron:Tuba1a 4:intergenic:Gm5581-Klra2 4:intergenic:Bpifa2-Gm21850 4:intron:Zkscan17 4:intergenic:Gm26126-Gm11114 4:intergenic:Gm23752-Gm24815 4:intergenic:Dnah1-Capn7 4:intergenic:Mir1942-Plec 4:intergenic:1600014K23Rik-Gm14765 3:intergenic:Gm13800-Gm13801 4:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:Vmn2r98-Vmn2r-ps119 4:intergenic:mmu-mir-6354-Gm5265 4:intergenic:4930506M07Rik-Vax1 4:intron:Pld5 4:intergenic:Mir466f-4-Irx1 4:intergenic:Ins1-Xpnpep1 4:intergenic:Gm24069-Gm24434 4:intron:Calcrl 4:intergenic:Vav2-AA645442 4:intergenic:Ranbp17/Gm12116-Ranbp17 4:intergenic:1700060C16Rik-Sox5 4:intron:Fanci 4:intergenic:Gm23591-Speer2 4:intergenic:Gm11846-n-R5s182 4:intergenic:Ank1/Gm15816-Nkx6-3 4:intron:Astn2 3:intergenic:Gm23035-Magi1 4:intron:Syt1 4:intron:Cubn 3:intron:Tyw5 4:intron:Depdc5 4:intergenic:Gpc5-4930505G20Rik 4:intergenic:Nox3-Gm23186 4:intergenic:Gm22245-1110034G24Rik 4:intron:Vmn2r13 4:intergenic:Gm25843-Tmeff2 4:intergenic:n-R5s94-Gm1840 4:intron:Setbp1 4:intron:Dock3 4:intergenic:Tmpo-Rmst 4:intron:Kcnd2 4:intergenic:Gm5205-Sh2d4b 4:intron:Clspn 3:intergenic:Gm9162-Scgb2b19 4:intron:Gm12695 4:intergenic:Gm14274-Mc3r 3:intergenic:Tmprss15-Rps19-ps12 3:intergenic:Gm6767-Cblb 4:intergenic:Gm5839-Gm25440 3:intron:Cyp2b10 3:intergenic:Gm26288-Atp10a 3:intron:Diap3 4:intron:Ptprm 4:intron:Ifit1 4:intergenic:Gm11997-Vwc2 4:intergenic:Gm22097-Gm23041 4:intron:Pikfyve 3:intron:Dennd4c 3:intergenic:Cdk1-Ank3 4:intergenic:Myzap/Gcom1-Cgnl1 3:intron:Srsf12 4:intergenic:Nrg1-Gm26578 4:intron:Gphn 4:intergenic:Gm24192-Gm12132 4:intergenic:Gm9387-Pitx2 4:intron:Camkk2 4:intron:Gm2016 4:intron:Gm5662 4:intron:Kdm5a 4:intron:Clec2g 4:intergenic:Atp8a2-Nupl1 3:intron:St5 4:intergenic:Gm2035-Gm2042 4:intergenic:Gm26674/Sash1-Gm9930 4:intergenic:Gm14679-4931400O07Rik 4:intergenic:Gpr150-Arsk 3:intron:Dlg1 3:intergenic:Akt3-Gm26801 3:intergenic:Gm26734-Gm23102 3:intergenic:B3galnt1-Gm25621 4:intron:Gm8300 4:intergenic:Gm8332-Gm5662 4:intron:Gm2022 4:intergenic:Gm22459-Gm25041 4:intron:Gm12796 4:intron:Crem 4:intergenic:Gm6180-Gm23531 4:intron:Ankrd13c 4:intergenic:Ankrd28-Gm22876 4:intergenic:Gm24031-Gm2178 4:intergenic:Ly86-Rreb1 4:intergenic:Pea15b-Gm22011 4:intron:Epha7 4:intergenic:Pcdh20-Gm25886 4:intergenic:Gm24998-Npy4r 4:intergenic:Gm9001-Gm22283 4:intergenic:Hey2-Gm24168 3:intergenic:Tm2d3-Gm7551 4:intron:Tmem255a 4:intergenic:Gm23760-Gm23013 4:intron:Galnt18 4:intergenic:Gm22218-Zmym2 4:intergenic:5930403N24Rik-Gm26535 4:intergenic:Gm26651-Diras2 4:intergenic:n-R5s94-Gm1840 4:intergenic:Adamts18-Nudt7 4:intergenic:Gm4968-Gm7308 4:intergenic:Abca8b-Abca8a 4:intron:St18 4:intergenic:Rit2-Gm23283 4:intergenic:Hs3st4/Gm27040-Gm25759 4:intergenic:Gm23180-Gm22977 4:intron:Pkhd1 4:intergenic:Sept5-Cldn5 4:intron:Ist1 4:intergenic:Ggps1-Ggps1/Arid4b 4:intron:Ttc28 3:intron:Lin28b 4:intron:Hdgfrp3 4:intergenic:Gm21399-Gap 4:intergenic:Sumo2-Nup85 4:intron:Myo10 4:intergenic:Gm5446-Elmo1 4:intron:Qser1 4:intergenic:1700026D08Rik-Mesdc1 4:intron:Lace1 4:intergenic:Gm25911-Gm24182 4:intergenic:Gm13090-Camta1 4:intron:Glt28d2 4:intron:Ptchd4 3:intergenic:Gm5860-Nfib 3:intergenic:4933402N22Rik-Sema3d 4:intron:Kdm4c 3:intron:Nrxn3 4:intron:Evc2 4:intergenic:Slc7a11-Gm24503 4:intron:Fmo2 4:intergenic:Gm23315-Rprm 4:intergenic:Gm10220-Gm7361 4:intron:Clpx 4:intergenic:Gm26005-Epcam 2:intron:Sorcs1 4:intergenic:Gm14989-Gm24675 4:intergenic:Slc6a3-Clptm1l 4:intergenic:Gm22273-Gm24079 4:intergenic:Tbc1d22a-Gm23416 4:intergenic:Gm23061-Ostf1 3:intron:Lrrc69 4:intergenic:Trerf1-Gm26216 3:intergenic:Gm13519-Gm13527 4:intergenic:Nrg3-4930529F24Rik 3:intergenic:Rnf144b-4931429P17Rik 4:intron:Tmem132d 4:intron:Gcsh 3:intron:Rabgap1l 3:intron:Herc2 3:intergenic:Prdx2-rs3-Dhrs3 2:intron:Pnpla8 3:intergenic:Lrfn3-Tyrobp 4:intergenic:Gm24633-Gm25568 4:intron:Fut9 4:intergenic:Gm24948-Nova1 4:intergenic:Gm11990-Pkd1l1 4:intron:4930595M18Rik 4:intron:Gm12130 3:intergenic:Gm13685-Zc3h15 3:intron:Slc9a9 4:intergenic:Dhdh-Tulp2 4:intron:Srgap1 4:intron:Tmem56 2:intergenic:Ndp-Efhc2 4:intergenic:Gm24350-Gm13464 3:intron:Rsrc1 4:intron:1110019D14Rik 4:intergenic:Mgam-Moxd2 4:intergenic:Gm23477-Gm10780 4:intergenic:Vps13c-Gm22382 4:intergenic:Gm23980-Gm22810 3:intergenic:Gm5776-Luzp2 4:intergenic:Ppp1cb-Gm15614 4:intergenic:Samd12-Gm26933 4:intron:Fryl 4:intron:Hsd17b13 4:intergenic:Adra2a-Gpam 4:intron:Rims1 4:intron:Cast 4:intergenic:1110036E04Rik-Scarletltr 4:intron:Bre Off-target primers show all... show less...
437 / fw	`ACTAAAGACTGGGCAGGGGT GGG` `.................... ...` Cloning / PCR primers	50	73	54	59	70	74	0 - 0 - 0 - 29 - 271 0 - 0 - 0 - 8 - 12 300 off-targets	4:intergenic:Gm5620-Zic1 3:intergenic:Hdac2-Marcks 4:intron:Kpna7 4:intergenic:Tmem229a-Gm24443 4:intergenic:Gm14907-Gm24683 4:intergenic:Gmps-Vmn2r-ps3 4:exon:Uap1l1 3:intron:Olfr411 4:intergenic:Reep5-Pkd2l2 4:intergenic:Ccdc104-Gm12085 4:intron:Vwa8 3:intron:Pcdh15 4:intergenic:Abra-Gm24751 4:intergenic:Gm26885-Pim1/Gm26885 4:intergenic:2810032G03Rik-Gm24758 4:intergenic:Lipo4-Lipo4/Lipo1 3:intron:Npas3 4:intergenic:Gm5335-A730056A06Rik 4:intergenic:Gm25644-Rpl31-ps18 4:intron:Rrbp1 4:intergenic:Gm11426-Cct6b 4:intron:Sobp 3:intergenic:Nav3-E2f7 4:intron:E2f2 4:intron:Rrbp1 4:intron:Rasgrf1 3:intron:Ifi205 3:intron:Cdc123 4:exon:Celsr3 4:intergenic:Erg-Ets2 4:intergenic:A930011G23Rik-Gm16228 4:intergenic:Dusp9-Pnck 4:intergenic:Ces1h-Ces5a 3:intron:Bmpr1a 4:intergenic:Gm6771-Ptchd4 4:intron:Spock1 3:intergenic:Gm26007-Gm7199 4:intergenic:Hivep2-Gm24343 4:intergenic:Gm22845-Nt5c1b 4:exon:Acaca 3:intron:Igsf21 4:intergenic:Alox12-Alox12e 4:exon:Fam189b 4:intergenic:Gm25204-n-R5s46 4:intergenic:Gm13988-Sema6d 4:intron:Ccdc11 4:intergenic:Gm11221-Gm11222 4:intergenic:Gm9897-Fzd1 4:intergenic:2010016I18Rik/Dennd2d-Dennd2d 4:exon:E130309D14Rik 4:intergenic:Chrm3-Gm26861 4:intron:Rai14 4:intergenic:Ddx11-Soga2 4:intron:Gsg1l 4:intergenic:Slc7a8-Gm8865 4:intergenic:Ctcf-Rltpr 4:intergenic:Gm22223-Gm22715 4:intergenic:Gm24587-Dcn 4:intergenic:Kcnma1-Gm6158 4:intron:Pard3b 4:intron:Dlgap2 4:intron:Slc2a13 4:intergenic:Cib4-Gm25666 4:intergenic:AC102758.1-Sox6 4:intergenic:Wdtc1-Slc9a1/Mir5122 4:intergenic:Galnt14-Ehd3 4:intergenic:Ctbp2-Tex36 4:intergenic:Gm22673-Gm23059 4:intron:Tmem184a 4:intergenic:mmu-mir-6343-Gm25974 4:intergenic:Dusp8-Gm25370 3:intergenic:Rpl38-Ttyh2 3:intergenic:Gm13780-Phf21a 4:intergenic:Ccdc71l-Nampt 4:intergenic:Gm22483-Prelid2 4:intergenic:Gm7168-Dact2 4:intron:Sec24d 3:intergenic:Gm12706-Ror1 4:intron:Ralgapa2 4:intron:Smok3b 4:intron:Smok3c 4:intergenic:Smok3a/Smok3b-Smok3a 4:intergenic:Gm13203-Casz1 4:intron:Mpp7 4:intron:Ndel1 4:intergenic:Gm872-Cdk17 4:intergenic:Tgfbr2-Rbms3 4:intergenic:Gm22642-Efna3/Gm15998 4:exon:Ucp2 4:intergenic:Dnajc12-Gm7075 4:intron:Psat1 4:intron:Syt16 4:intergenic:Nek10-Lrrc3b 4:intergenic:Lrrc32-Gm23040 4:intron:Ntrk2 4:intergenic:Hspa8/Snord14c/Snord14d/Snord14e-Bsx 4:intron:Zbtb7c 4:intron:Tspan11 4:intergenic:Tmpo-Rmst 4:intron:Bcl7c 4:intergenic:1110008E08Rik-Gm23406 4:intron:Chst9 4:intron:Nbea 4:intron:C230014O12Rik 4:intron:Depdc5 3:intron:Ctnna1 4:intergenic:Gm14621-Hs6st2 4:intron:Cntn4 4:intron:Mzt2 4:intergenic:4833412C05Rik-Igf1r 4:intergenic:Gm14824-Mir384 4:intron:Cep290 4:intergenic:Gm27037-Gm23859 4:intron:Xylt1 4:intron:Abca1 4:intron:Fmn1 4:intergenic:1700003H04Rik-Gm23673 3:exon:Zfp523 4:intergenic:Nxn-Timm22 4:intergenic:Gm5558-Rchy1 4:intergenic:Gm22868-Fancl 4:intron:Efnb2 4:intergenic:Ccnd1-Tpcn2 4:intron:Sycp2l 3:intergenic:Bglap-Pmf1 4:intron:Col4a6 4:intergenic:Kcnk1-Slc35f3 4:intergenic:Grik3-2610028E06Rik 4:intergenic:Pik3cd-Tmem201 4:intron:Itih2 4:intergenic:Gm23072-Gm13469 4:intergenic:Gm15686-Gm7518 4:intron:Coro6 3:intergenic:Gm16863-Vps8 4:intergenic:Gm13115-BC039966 4:intergenic:Gm10827-Nhsl1 4:intergenic:Smug1-Cbx5 4:intron:Kif1c 4:intergenic:Gm11351-Gm11352 4:intergenic:Atp1a1-Mab21l3 4:intergenic:Irx1-Rpl9-ps4 4:intergenic:Mocs1-Daam2 4:intergenic:Tmpo-Rmst 4:intergenic:Cdh4-Gm14300 4:exon:Zfp689 4:intergenic:Ryr2-Mtr 4:intergenic:Gm826-Mafb 4:intergenic:F2rl1-F2r 4:intergenic:Gm13940-Katnbl1/Gm21985 4:exon:Olfr960 4:intron:Siglec1 4:intergenic:Rfwd2-Gm10530 4:intron:Wfikkn1 4:intron:Ano10 4:intergenic:B230206H07Rik-Gm25725 4:intergenic:Efna5-Fbxl17 4:intergenic:Popdc2-Pla1a 3:intergenic:Rpl30-ps8-Foxn3 4:intergenic:Phf3-Ptp4a1 4:intron:Lsamp 4:intergenic:Grin2a-Gm7572 4:intergenic:Gm22016-Cdc73 4:intron:Spdef 4:intergenic:Gm17033-4933406K04Rik 4:intergenic:Gm5123-Dusp21 4:intergenic:Gm5082-Adtrp 4:intron:Fto 4:exon:Gtpbp2 4:intergenic:Gpatch2-Esrrg 4:intergenic:Tnks/Gm9939-Rpl31-ps23 4:intergenic:4922501C03Rik-Tbx18 4:intron:4930517O19Rik 4:exon:Fzd8 4:intergenic:Ptprs-Znrf4 4:exon:Nfatc2 4:intron:Bace2 4:exon:Adnp2 4:intron:Tmem2 4:intergenic:mmu-mir-6343-Gm25974 4:intergenic:Fut8-Gm24070 4:intergenic:Rps6-ps2-mmu-mir-8110 3:intergenic:Gm26933-Tnfrsf11b 4:intergenic:Gm9972-Gm12145 4:intron:Tenm1 4:intergenic:Mir688-Calcoco1 4:intron:Rmi2 4:intron:Mapkap1 4:intron:Cngb3 4:intergenic:Gm14847-4930519F16Rik 4:intron:Tm9sf3 4:intron:Zbtb20 4:intron:Exoc4 4:intron:Rpn2 4:intergenic:Sspo-Gm10243 4:intergenic:Triml1-Triml2 4:intergenic:Rpl21-ps13-mmu-mir-6401 3:exon:Gm20654 4:intergenic:Gm23105-Pawr 4:exon:Kmt2d 4:intron:Dock1 4:intergenic:Ptprn2-Rapgef5 3:intergenic:A730085K08Rik-9530059O14Rik 4:intron:Nkd1 4:intergenic:Sept9-Gm16045 4:intergenic:Slc24a3-Gm14093 4:intergenic:Gm26455-Gm15754 4:intergenic:Gm13794-Gm13796 4:intron:Cacna1c 4:intergenic:Sp7-Gm23590 4:intergenic:Rnf144b-4931429P17Rik 4:intergenic:Gm26111-Gm14667 4:intron:Dclk2 3:intergenic:Dmrt3-Dmrt2 3:intergenic:Gm15755-Snap47 4:intron:Olfm3 3:intron:Nfatc2 4:intergenic:Col22a1/Gm16308-Gm16308 4:intron:2610307P16Rik 4:intergenic:Gm24633-Gm25568 4:intergenic:Gm15521-Ntm 4:intergenic:Slc7a8-Gm8865 3:intron:Aifm1 4:intron:Cdkl5 4:intergenic:Gm22521-Gm25702 4:intron:Fetub 4:intergenic:Rmi1-Slc28a3 4:intron:Ddx4 4:intron:Mfi2 3:intron:Upf2 4:intergenic:Olig3-Ifngr1 4:intron:Nt5c2 3:intron:Ceacam5 3:intergenic:Polr1d-Gsx1 3:intron:4930441J16Rik 4:intergenic:Cbln2-Gm5096 4:intergenic:Nrn1-F13a1 4:intergenic:AF529169-Tmed3 4:intergenic:Gm23681-Peli1 4:intron:Rps6ka2 4:intergenic:Ttc6-Sstr1 4:intron:Kif21b 4:intergenic:Gm16391-Gm17538 4:intergenic:Gm22797-Robo1 4:intergenic:Ryr3-Tmco5b 4:intron:Dock1 4:intergenic:Sub1-Zfr 4:intergenic:Prkca-Gm11655 4:intergenic:Trpm6-Rorb 4:intron:Kcnt2 4:intergenic:Gm22886-Klhl14 4:intron:Adck3 4:intergenic:Tbc1d5-Satb1 4:intergenic:Gm24404-Nrg3 4:intron:Veph1 4:intergenic:Wac-Gm5819 4:intergenic:Kcnq5-Gm26580 4:intergenic:Gm25041-Gm25853 4:intergenic:A730085K08Rik-9530059O14Rik 4:intergenic:Gja1-Gm9795 4:intron:Creb3l2 4:intron:Cep128 4:intergenic:1700019E08Rik-Gm13465 4:intergenic:Gm25818-Creb5 4:intergenic:Cct8l1-Gm10062 4:intergenic:Gm22346-Mcfd2 4:intergenic:Gm24591-Gm26241 4:intergenic:Tcerg1l-Mapk1ip1 4:intergenic:Hipk2-Tbxas1 4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Gm12066-Gm12068 4:intron:Zfp618 4:intron:Trim14 4:intron:Shroom3 4:intergenic:Gm23185-Gm23848 4:intergenic:AC102758.1-Sox6 4:intergenic:Fam103a1-3110040N11Rik 4:intergenic:Cfhr3-Gm26271 4:intergenic:Gm15224-Gm15225 4:intron:Fmod 4:intron:Cyp4f40 4:intergenic:Gm16201-Gm2044/Mir202 4:intron:Agbl4 4:intron:Erp44 4:intergenic:Pogk-Tada1 4:intergenic:Snx19-Adamts15 4:intergenic:n-R5s96-Cldn24 4:intergenic:Slc5a7-Trp53-ps 4:intergenic:Gm23437-Gm24818 4:intron:Lrig1 4:intergenic:Twist2-Hdac4 4:intergenic:Cadm1-Nxpe2 4:intergenic:Ostm1-Gm15199 4:intergenic:Gm22727-Gm4258 4:intron:Adhfe1 4:intron:Thsd7b 4:intergenic:Gm14612-Gm22288 4:intergenic:Lect2-Tgfbi 4:intron:Kcnk10 4:intergenic:Gm12213-mmu-mir-7671 4:intergenic:Mrc1-Slc39a12 Off-target primers show all... show less...
433 / fw	`ATAAACTAAAGACTGGGCAG GGG` `.................... ...` Cloning / PCR primers	49	58	60	52	68	78	0 - 0 - 5 - 38 - 343 0 - 0 - 0 - 5 - 4 386 off-targets	3:intron:Tomm40 4:exon:Ttyh1/D030047H15Rik 3:intron:Lef1 3:intergenic:Gm10451-Mir5135 4:intron:Scarb1 3:intron:Sulf2 3:intergenic:Rpl21-ps5-Gm24508 4:exon:Rpl7l1 4:intergenic:Gm25598-BC002059 4:intron:Adra1b 4:intron:Kif3c 4:intron:Csmd1 3:intergenic:Cdh10-Gm25976 4:intergenic:Ugcg-Gm12596 4:intergenic:Gm4487-Gm24073 4:intron:Kctd13 4:intergenic:Gm23337-Gm26081 4:intergenic:Acyp2-Gm12099 4:intron:Gm12239 4:intron:Hecw1 4:intron:Arhgap42 4:intergenic:Rasgrf2-Msh3 4:intergenic:Kcng3-Mta3 4:intergenic:Gm21971-AK010878/Gm20604/Moap1 4:intergenic:Gm25671-Gm16387 4:intergenic:Gm22638-Gm6605 4:intergenic:Urgcp/Mrps24-Urgcp 4:intergenic:Gm15401-Tcf7l1 4:intergenic:Rbm28-Gm24303 4:intergenic:Gm14983-Diap2 4:intergenic:Grb14-Cobll1 4:intergenic:4933416E03Rik-9130015L21Rik 4:intron:Phtf2 4:intron:Psma6 3:intergenic:Lsm3-Slc6a6 3:intron:Kdm5d 4:intergenic:Rps19-ps8-Mtf2 4:intron:Mtdh 4:intergenic:Itih5-Gm13261 4:intron:2310035C23Rik 3:intron:Ptprc 4:intergenic:Gm15327-Gm15325 4:intron:Gmds 4:intergenic:Isoc1-Adamts19 4:intergenic:Gm22084-Gm24502 4:intergenic:9930038B18Rik-Laptm4a 4:intergenic:Cpq-Gm24476 3:intergenic:Gm25824-Gm26977 4:intron:Mcm9 4:intron:Slc25a21 4:intron:Slc6a12 4:intergenic:Rab11a-Dennd4a 4:intergenic:Frem2-Gm24851 4:intergenic:Cacna2d3-Selk 4:intergenic:Bcar1-Cfdp1 3:intron:Upf2 4:intron:Slbp 4:intron:Rbm26 4:intergenic:Dgkk-Ccnb3 4:intergenic:Nrxn1-Gm20491 4:intron:Palld 4:intergenic:Smg7-Nmnat2 4:exon:Fzd6 4:intron:Papolg 4:intergenic:Tdrd3-Rps3a2 4:intron:9430041J12Rik 4:intergenic:Gm14012-Gm14005 4:intron:Upf2 4:intron:Neb 4:intergenic:Ptma-Pde6d 4:intron:Myt1l 3:intron:Hrk 3:intergenic:Rpl21-ps13-mmu-mir-6401 4:intron:Scfd1 4:intron:Khdrbs2 4:intergenic:RP24-325L16.1-RP23-417D20.1 4:intergenic:Atp6v1h-Oprk1 4:intron:Anks1b 4:intergenic:Klrb1-ps1-Gm26160 4:intergenic:Spata18-Usp46 4:intergenic:1500009L16Rik-Nuak1 4:intergenic:Gm25609-Gm23534 4:intergenic:Fmr1-Fmr1nb 3:intergenic:Nr5a2-Gm23763 4:intergenic:Gm12746-Ssbp3 4:intergenic:Cul5-Cul5/Gm16124 4:intergenic:Gm22603-Gria1 4:intron:Bai3 4:exon:Alg13 4:intergenic:Pglyrp4-Gm5849 4:intergenic:Trit1-Gm12923 4:intron:Ncbp1 4:intergenic:Nmur1-1700019O17Rik 4:intergenic:Cap1-Gm12891 4:intergenic:Pla2g4e-Pla2g4e/Gm13997 4:intergenic:Prg4-Hmcn1 4:intron:Rin2 4:intergenic:Lcn10-Obp2a 4:intron:Prom1 4:intergenic:Pdha1-Gm15195 2:intergenic:Lgals4-Lgals7 4:intergenic:Gm13616-Ttc21b 4:intergenic:Ntf3-Kcna5 4:intergenic:1500009L16Rik-Nuak1 4:intergenic:Olfr1302-Olfr1303 4:intergenic:Gm23263-Gm13005 4:intergenic:Gm23649-Lclat1 4:intron:Afap1 4:intron:Adprhl1 4:intron:Rdh18-ps 4:intron:Rdh16 4:intron:Eml1 2:intron:Nwd1 4:intergenic:Eef1e1-Gm22035 4:intron:Mthfd2l 4:intron:Parp4 4:intergenic:Colec11-Rps7 4:intergenic:Gm15950-Mpv17l 3:intergenic:Gm8991-Gm6673 4:intron:Rtn2 4:intergenic:Krt42-Gm14206 4:intergenic:Gm4791-Gm22286 4:intron:Dnm3 4:exon:Lrrc58/Gm15725 4:intron:Agfg1 4:intergenic:Mir466j-Gm20611 4:intergenic:AC158354.1-Gm4804 4:intergenic:Slitrk6-Gm22802 4:intron:Ptpn11 4:intron:Sil1 4:intron:Grik2 4:intergenic:mmu-mir-6238-AC161571.1 4:intron:Pcdh7 4:intron:Zbed4 4:intergenic:Gm24127-Pcdh9 4:intergenic:Gm13685-Zc3h15 4:intergenic:Plcl1-Hsfy2 4:intergenic:Gm3898-Gm23326 4:intergenic:Gm25513-Gm15975 4:intron:Aoah 4:intron:Cdyl2 3:intergenic:Cnot2-5330438D12Rik/Cnot2 4:intergenic:E330017L17Rik-Spocd1 4:intergenic:Mib1-1010001N08Rik 4:intergenic:Gm26005-Epcam 4:intergenic:Gm10110-Gm25495 4:intergenic:Mup-ps8-Gm21286 4:intergenic:Gm13773-Gm12567 4:intergenic:Vmn2r-ps119-Vmn2r-ps120 4:intron:Cntn5 4:intergenic:Arhgap25-Prokr1 4:intron:Brinp3 4:intron:Ankhd1 3:intron:Nfia 4:intergenic:Rnf144a-Rsad2 4:intergenic:Gm14550-Gm14570 4:intron:Cdh2 4:intergenic:Peli2-Gm23881 4:intron:Gm2716 4:intergenic:Ncoa1-Gm3625 4:intergenic:Adss-Gm16432 4:intergenic:Qrfpr-Anxa5 4:intergenic:Gm14698-Gm6812 4:intergenic:4833412C05Rik-Igf1r 3:intergenic:Ninj1-1110007C09Rik 4:intergenic:Gm5601-Acsm3 4:intron:Mast1 4:intergenic:Cxxc5-Gm23882 4:intergenic:Kit-Gm25374 4:intergenic:Dock9-Ubac2 4:intergenic:Gm1527-Egfem1 4:intron:Nup62cl 4:intergenic:Gm23534-Gm22727 4:intron:Ppp6r3 4:intergenic:Rpl21-ps4-Gm3839 4:intergenic:Gm12947-4930471C06Rik 4:intergenic:Bbs9-U4 4:intergenic:Gm7611-Aoah 4:intron:Klhl23 4:intron:Ubn1 4:intergenic:Mir3475-Frmpd3 4:intron:Anks1b 3:intergenic:Gm5294-Pdgfa 4:intron:Scp2 4:intergenic:Lrfn2-Gm22978 4:intergenic:Ddx46-B230219D22Rik 4:intron:Cacna1c 4:intron:Agbl4 4:intergenic:Gm21704-Gm3376 4:intergenic:Gm3376-Gm8521 4:intron:Phf16 4:intergenic:Gm24793-Wdr59/mmu-mir-1957b 4:intron:2010109A12Rik 3:intergenic:Ptprn2-Rapgef5 4:intergenic:Axin2-Gm11668 4:intron:Rb1 4:intergenic:Gm8214-Dusp10 4:intergenic:Npbwr1-Rb1cc1 4:intergenic:Gm15208-Bcl11b 4:intergenic:Ncam2-Gm23355 4:intron:Psap 4:intron:Ubqln1 4:intergenic:Setdb1-Gm5070 4:intergenic:Syf2-Gm16225 4:intron:Fbln2 4:intergenic:Rpl31-ps8-Tcea1-ps1 3:intergenic:Gm7931-Slit2 4:intergenic:Mamdc2-1700028P14Rik 4:intergenic:Gm1818-Gm26454 4:intergenic:Gm15208-Bcl11b 4:intron:6430548M08Rik 4:intergenic:Gm26820-Tacr3 4:intergenic:Gm22674-Cdyl 4:intron:Ctnna1 4:intergenic:Gm16391-Gm17538 4:intergenic:Arap3-Pcdh1 4:intergenic:4930556G01Rik-Gm25705 4:intergenic:Hsd17b6-Prim1 4:intergenic:Gm22360-Gm24586 4:intergenic:Rpl12-ps1-n-R5s210 4:intergenic:Gm12312-Pelp1 4:intron:Mfi2 4:intergenic:2610028E06Rik-Csf3r 4:intergenic:Sox2ot-mmu-mir-6378 4:intron:Lsamp 4:intergenic:Gm14521-Gm14635 3:intergenic:Psors1c2-Cdsn 4:intron:Pcsk5 4:intron:Nudcd1 3:intergenic:Exoc4-Gm13854 4:intron:Cage1 4:intron:Gkap1 4:intergenic:Gm9884-Gm24064 4:intergenic:Gm13517-Gm13615 4:intron:Kcnd1 4:intron:Kcnd3 4:intron:BC053393 4:intergenic:Gm9929-Dusp12 4:intron:Kremen1 3:intron:Tmem132a 4:intergenic:Lhx4-Qsox1 4:intergenic:Gm25267-Il15 4:intergenic:Fam76b-Sesn3 4:intergenic:Gm16118-Wwox 4:intergenic:Ryr2-Mtr 4:intergenic:ATF7/Atf7-ATF7 4:intergenic:Gm26521-5730577I03Rik 4:intergenic:Rasgrf2-Msh3 4:intron:Cux1 4:intron:Setdb1 4:intron:Skint5 4:intergenic:Gm5494-Gm20580 2:intron:Magi1 4:intergenic:Zc3h12a-1700041M05Rik 4:intergenic:4930555F03Rik-Gm23986 4:intergenic:Nmd3-Sptssb 4:intergenic:Tmed10-Fos 4:intergenic:Ddx51-Ep400 3:intergenic:Zg16-Gm22747 4:intron:E230019M04Rik 4:intergenic:Gm12816-Gm12818 4:intron:Cds1 4:intergenic:Gm22276-Gm9825 4:intergenic:Gm10313-Gm24669 4:intron:4930529F24Rik 4:intergenic:Gm22536-Gm6632 4:intron:Vmn2r26 4:intergenic:Ncam2-Gm23355 4:intron:Chst11 4:intergenic:Gm24035-Cdh13 4:intron:Hmcn2 4:intron:Synj2 3:intergenic:1500009L16Rik-Nuak1 4:intron:Atg7 4:intergenic:Foxn3-Gm19898 4:intergenic:Pcsk1-Gm4149/Mir682 4:intergenic:Adh6b-Adh6-ps1 4:intron:Acbd6 4:intergenic:Gm5941-Mageb18 4:intergenic:Acbd6-Lhx4 4:intron:Atxn1 4:intergenic:Ier5l-Gm14487 4:intron:Nrp2 4:intergenic:Ust-Gm24374 4:intron:Fstl5 4:intergenic:Fam58b-Gm23840 4:intergenic:Wsb1-Gm9964 4:intergenic:Gm14055-Gm14064 4:intergenic:Gm22149-Rgs6 4:intergenic:Gm22016-Cdc73 4:intron:Eefsec 4:intergenic:4930474N05Rik-Gm24394 4:intron:Scfd2 4:intron:Ttc23 3:intron:Rtn4 4:intron:Abca12 4:intergenic:Gm26861-Gm23415 4:intergenic:Rapgef5-Gm24741 4:intergenic:Gm26970-Foxk1 4:intergenic:Nt5c2-Ina/Nt5c2 3:intergenic:Impg1-RP24-325L16.1 4:intergenic:Csf1-Eps8l3 4:intergenic:Gm6910-Gm10324 4:exon:Rybp 3:intergenic:Gm10398-Gm26660 4:intergenic:Pomc-Efr3b 4:intergenic:Gm4791-Gm22286 4:exon:Csrnp3 4:intergenic:Gm6419-Rtdr1 4:intergenic:Gm9621-Gm24200 4:intron:Atp8b3 4:intergenic:Casp16-Zfp213/Gm26695 4:intergenic:Gm13368-Gm13364 4:intron:Stim1 4:intergenic:Gm22886-Klhl14 4:intron:Ttc21a 3:intron:Met 2:intron:Abca4 3:intergenic:Eif4g2-Galnt18 4:intergenic:Gm12726-Gm12728 3:intron:Shroom3 4:exon:Man2c1 4:intron:Eya4 4:intergenic:L3mbtl3-Gm8709 4:intron:Prkcb 4:intergenic:Irx1-Rpl9-ps4 4:intergenic:Gm14261/Sall4-Gm25214 4:intergenic:Map2k1-Gm24106 4:intergenic:Ggact-Pcca 4:intergenic:Lcorl-Gm7931 3:intron:Dcdc5 3:intergenic:Guk1-2610507I01Rik 4:intergenic:Sec11a-Zfp592 4:intergenic:Gm22460-Akr1c14 4:intron:D630045J12Rik 4:intergenic:Cnksr2-Gm8659 3:intergenic:Rpl21-ps7-Eml4 4:intergenic:Rp1-Sox17 4:intergenic:Mvk-Gm13790 4:intron:Tmem241 4:exon:Aco1 2:intergenic:Kif14-Gm26598 4:intergenic:Skint11-Trabd2b 4:intergenic:Tpx2-Mylk2 3:intergenic:Rbfox1-Gm23476 3:intergenic:Ttc32-Gm7099 4:intergenic:Nxn-Timm22 4:intron:Gabra5 4:intergenic:Rarb-Thrb 4:intergenic:1700041M05Rik-Grik3 4:intron:Daam2 4:intron:Ctnna3 4:intergenic:Dlx1os-Metap1d 4:intron:Cttn 4:intergenic:Gm14161-Gm17416 3:intergenic:A530064D06Rik-Trem1 4:intergenic:Gm22159-Gm25293 4:intergenic:Gm25756-Gm22553 4:intergenic:Gm4875-Rasgef1a 4:intergenic:Gm11746-Slc24a3 4:intron:Myrip 4:intergenic:Gm26923-Gm25143 4:intron:Adam28 4:intron:Kif16b 4:intergenic:Papd7-Papd7/Nsun2 4:intron:Tenm1 4:intergenic:Gm13660-Gm13655 4:intergenic:Cmpk2-Gm9866 4:intron:Usp40 4:intergenic:Ppa1-Sar1a 4:exon:Mpc2 4:intergenic:Gm16210-Rev3l 4:intergenic:Fam184a-Man1a 4:intron:Ntm 4:intergenic:Gm25476-Gramd3 4:intergenic:Gm15530-Arhgap31 4:intron:Bmpr1a 4:intergenic:Park2-Agpat4 4:intergenic:Sash1-Gm26674 4:intron:Rasgrp1 4:intron:Cers6 4:intron:Scaper 4:intergenic:Gm26054-Xylt1 4:intergenic:Gm25598-BC002059 4:intergenic:Gm15262-Prkaca-ps1 4:intergenic:Gm25103-Dph6 Off-target primers show all... show less...
133 / rev	`TCAAGTTAGGCCAGTCATGG TGG` `.................... ...` Enzymes: NlaIII Cloning / PCR primers	47	78	67	57	58	78	0 - 0 - 4 - 49 - 288 0 - 0 - 1 - 5 - 5 341 off-targets	3:intergenic:Gm8991-Gm6673 3:intron:Gtf2e2 4:intron:Osbpl1a 4:intergenic:Gm805-Gpatch2l 3:intron:Gm6999 4:intergenic:Olfr284-Olfr283 4:intron:Rap1gds1 4:intergenic:Sec11a-Zfp592 4:intergenic:Rilpl2-Gm15621 4:intron:Usp9x 4:intron:Fat1 4:intron:Pcdh11x 3:intron:Zzef1 3:intergenic:Tas2r122-Tas2r115 3:intron:Ncoa2 4:intergenic:Thumpd2-Slc8a1 3:intergenic:Xpnpep2-Sash3 4:intron:Ncoa7 4:intron:Grik2 4:intron:Wdr7 3:intron:Tpk1 4:intergenic:Ngdn-Gm10364 4:intergenic:Gm23860-Gm11800 3:intergenic:Cpa6-Gm15603 4:intron:Slc7a8 4:intron:Upf1 4:intergenic:Gm25623-Gm12753 4:intron:Prkcb 4:intron:Asic2 3:intergenic:Veph1-Shox2 4:intergenic:Gm13421-Rxra 4:intron:Unc13c 4:intron:Ago3 4:intergenic:Gm25293-Cdh7 2:intergenic:Gm11625-Brca1 3:intergenic:Gm7146-Klhl4 3:intergenic:Gm23799-Gm25917 4:intergenic:Gpr165-Gm16394 4:intergenic:Gm24586-Gm22917 3:intergenic:Gm8325-P2ry1 4:intron:Txlng 4:intergenic:Gm22469-Gm23909 4:intergenic:Chat-Gm2990 4:intergenic:PCGEM1-Gm25843 4:intergenic:Vmn1r229-Vmn1r230 4:intron:Myo1e 4:intergenic:Nsmce1-Il4ra 2:intergenic:Pabpc2-Yipf5 4:intron:Enox1 4:intron:Ppap2c 2:intron:Elmo1 4:intergenic:Grin3a-Gm12458 4:intergenic:Gm14062-Btbd3 4:intron:Papd4 4:intergenic:4933406K04Rik-1700013N06Rik 4:intron:Nav2 4:intergenic:Gm25650-Gm23584 4:exon:Gm22334 4:intergenic:5031410I06Rik-Gm10220 4:intergenic:Gm21698-Gm1979 4:intergenic:Gm7347-Gm10471 4:intron:Trerf1 4:intergenic:Adra1a-Gm10032 3:intergenic:Olfr472-Olfr473 3:intergenic:Olfr136-Gm20408 4:intron:Rap1a 4:intergenic:Hmgb1-5730422E09Rik 4:intergenic:Tmprss15-Rps19-ps12 4:intron:Epsti1 4:intergenic:BC052040-Meis2 4:intergenic:Gm19965-Gm24030 4:intergenic:Gm15160-Gm15155 3:intergenic:Vmn1r64-Gm20399 3:intergenic:Vmn1r62-Vmn1r63 3:intergenic:Gap-VMN1R62 4:intergenic:Rps11-ps3-Plekhf2 4:intron:4922501C03Rik 3:intron:Naf1 4:intron:Slc24a4 4:intergenic:Gm11668-Gm24149 4:intergenic:BC021785-AI317395 4:intron:Hdac2 4:intron:Fgf14 3:intergenic:Gm25684-Gm9966 3:intron:Ptprq 4:intergenic:Gm23591-Speer2 4:intergenic:Uchl5-Rgs2 4:intron:1110051M20Rik 3:intergenic:Gm24731-Gm22076 4:intron:Wwc1 3:intron:Vmn2r79 3:intergenic:Gm24080-Gm22104 3:intron:Vmn2r78 3:intergenic:Sntb2-Gm16208 4:intron:Col24a1 4:intergenic:AC175117.1-Hnf4g 4:intron:Etf1 4:intergenic:Foxf2-Gm25186 4:intergenic:RP23-452E7.2-Irak1bp1 3:intron:Sfpq 4:intergenic:Gm25614-Gm9022 4:intergenic:Synpr-Gm15913 4:intergenic:Gm25665-Gm24469 4:intron:Fhod3 4:intergenic:Pabpc6-Qk 4:intron:Cask 4:intergenic:Gm9083-Slc9a7 4:intergenic:Gm13486-Gm13483 4:intergenic:Gm23955-Sv2c 4:intron:Nutf2 4:intergenic:Gm5506-Gm5839 4:intergenic:Gm6277/Cables1/Mir1901-Cables1 4:intergenic:Hif1an-Gm26644 4:intergenic:Gm23036-Gm22034 4:intron:Fstl4 4:intergenic:Ntf3-Kcna5 3:intron:Gfra1 3:intergenic:Gm23319-Spata9 3:intron:Slc5a4b 3:intergenic:Gm10051-Gm15627 4:intron:Abhd17c 4:intron:Hvcn1 4:intron:Col6a2 4:intergenic:Pomgnt2-Snrk 4:intergenic:Gm25659-Gm25644 4:intergenic:Gm24092-Cenpf 4:intergenic:Eepd1-Gm26190 4:intergenic:Gm25756-Gm22553 4:intergenic:Gm25437-Gm22684 4:intergenic:Gm14947-Gm14940 3:intergenic:Gm9726/mmu-mir-8099-2-Gm26055 4:intron:Pard3b 4:intergenic:Veph1-Shox2 4:intergenic:Gm11612-2410003L11Rik 4:intergenic:Cep55-Ffar4 4:intergenic:Gm24805-Sfr1 4:intergenic:Slc35a3/Hiat1-Slc35a3 4:intergenic:Ankrd66-Pla2g7 4:intergenic:Gm7353-AU018091 4:intron:Irf2 4:intergenic:Gm22223-Gm22715 4:intron:Gm15155 4:intergenic:Gm8244-Gm6754 4:intergenic:Gm13461-Gm13463 4:intron:Gne 4:intergenic:Lrrc72-Sostdc1 4:intergenic:Eln-Wbscr28 4:intergenic:Gm14505-Gm9435 4:intergenic:Gm23681-Peli1 4:intergenic:Gm9001-Gm22283 4:intergenic:Klhl15-Klhl15/mmu-mir-7673 4:intergenic:Slco3a1-Gm22326 4:intergenic:Npvf-G930045G22Rik 4:intergenic:Grk5-Gm9529 4:intron:Cnot6l 4:intergenic:Ccbe1-Mir694 4:intergenic:Gm9075-1700012B15Rik 4:intergenic:Gm23029-Gm14937 4:intergenic:Gm7275-Gm7204 4:intergenic:Pdgfc-Gm22531 4:intergenic:Gm25813-Gm23666 4:intergenic:Ncam2-Gm23355 4:intergenic:Dtd1-Gm11743 4:intron:Gpr125 2:intron:Acot7 4:intergenic:Gm6098-Zbbx 3:intron:Map2k4 3:intergenic:1700018C11Rik-Gm24234 4:intergenic:Fcho2-Tnpo1 4:intergenic:Gm6984-Htr2a 4:intron:Cstad 4:intron:RP23-302F9.3 3:intron:Malsu1 4:intergenic:Evi5-Ube2d2b 4:intron:Zmat3 4:intron:Atp6v1h 4:intron:Mfap1a 4:intron:Mfap1b 4:intergenic:Rhbdf2-Cygb 4:intergenic:Gm12033-Gm12034 4:intergenic:Gm8312-1700036O09Rik 4:intergenic:Lrrc8d-Rps15a-ps5 4:intergenic:Chrm1-Chrm1/Gm22820 4:intron:Sart3 3:intergenic:Ccdc50-Fgf12 3:exon:Apobec3 4:intergenic:Pabpc4l-mmu-mir-6379 4:intergenic:Gm15399-Gm9372 4:intergenic:Il9-Fbxl21 4:intergenic:Gm25824-Gm26977 3:intergenic:Gm20713-Gpc5 3:intergenic:Slc9a9-Chst2 4:intergenic:Gm15060-U6 4:intergenic:Gm10067-Lig4 4:intron:Myo3a 3:intron:Msi1 4:intron:Ube2l3 3:intergenic:Gm26180-Anks1b 4:intergenic:Crem-Gm6225/Crem 4:intergenic:Dcaf17-Cybrd1 4:intergenic:Gm22325-A830052D11Rik 4:intron:Bmp2k 4:intron:Bmpr1b 4:intergenic:Gm11624-Efcab3 4:intergenic:Ccr7-Smarce1 4:intron:Rasgef1c 4:intergenic:Gm13209-Srm 4:intergenic:Gm12024-Gm12023 4:intron:BC053393 4:intergenic:Rasgef1c-Rnf130 4:intergenic:Bbx-G730013B05Rik 4:intergenic:Lrriq1-Slc6a15 4:intergenic:Hdhd1a-Prr16 4:intron:Phf21b 4:intron:Gm10283 4:intergenic:A630012P03Rik-Mir363 4:intergenic:Gm20397-Rbms3 3:intron:Ube2h 4:intergenic:Ncor1/Pigl-Pigl 4:intron:Sgms1 4:intergenic:Rpl6-Gm15800 4:intergenic:Pcdh1-2010320O07Rik 4:intron:Sox13 4:intron:Gm12963 4:intergenic:Vegfa-Mrps18a 4:intron:Myo1f 4:intergenic:Znrf3-Gm11962 4:intron:Myo9a 4:intergenic:Gm24917-Commd1 4:intergenic:Med6-Ttc9 4:intergenic:Gm15385-Vbp1 4:intron:Abl1 4:intron:Vwa3a 4:intergenic:Gm13811-Gm13813 4:intergenic:Cyb5-Fbxo15 4:intron:Pde6d 3:intron:Atp6ap2 4:intron:Vps8 4:intron:Aldh1l1 4:intron:Pole 4:intergenic:Dpcr1-Sfta2 4:intron:Add1 4:intergenic:Tnk1-Tmem95 3:intergenic:Gm26883-Gm26883/Tshz2 4:intergenic:Gm25509-n-R5-8s1 4:intergenic:Zfp219-Olfr221 4:intergenic:Gm26889/Svep1-Musk 4:intergenic:Gm4944-Zfp944 4:intergenic:Gm26204-Gm9790 4:intron:Fam169a 4:intergenic:Colec10-Mal2 4:intergenic:Spry1-Gm5148/Rps23-ps 4:intergenic:Gm25261-Gm831 4:intron:Mitf 4:intron:9230106D20Rik 4:intron:2410076I21Rik 4:intergenic:RP23-220D12.12/Rbm12/Cpne1-Cpne1/Rbm12/RP23-220D12.12 4:intron:Tlk1 4:intergenic:Gm22492-Pdcd6ip 4:intergenic:Gm6225-Cul2 4:intergenic:Rpl15-ps2-Tcf12 4:intergenic:Gm4991-Gm7134 4:intergenic:Ltk-Rpap1 4:intron:Timm23 4:intergenic:Slc4a8-Scn8a 4:intron:Lmx1a 4:intron:Kcnq5 4:intergenic:Mir1931-Cdk17 4:intron:Pcca 4:intron:Adgb 4:intergenic:Gm24680-4921530L21Rik 4:intron:Smad9 4:intron:Racgap1 4:intron:Otud6b 4:intergenic:Syncrip/Gm20537-Syncrip 4:intergenic:Gm24246-Pik3c3 4:intron:Atp10b 4:intergenic:Gmnc-Ostn 4:intergenic:Gm1815-Zfp353-ps 4:intergenic:Gm16035-Gm24131 4:intergenic:Zfp516-Gm24720 3:intergenic:Aim1l/Cd52-Aim1l 4:intergenic:Gm9725-Gm4899 4:intergenic:Gm13211-Gm13254 4:intergenic:Gm13714-Olfr987 4:intron:Gmeb1 4:intron:Gli3 4:intergenic:Anxa10-Gm22324 4:intron:Dus4l 4:intergenic:Nacad-Tbrg4 4:intergenic:4930527B05Rik-1700071K01Rik 4:intergenic:Gm11849-Gm23384 4:intergenic:Gm23943-4930558J18Rik 4:intergenic:Paqr3-Gm5560 4:intergenic:Magea4-Gabre 4:intron:Heatr3 4:intergenic:Dpyd-Gm23432 4:intron:Epb4.1l4a 4:intron:Lgals8 4:intergenic:Gm24774-Pcdh17 4:intergenic:Atp1a1-Mab21l3 4:intron:Cabin1 4:intergenic:Gm9621-Gm24200 4:intron:Tc2n 4:intergenic:Agpat9-Gm20484 4:intergenic:Gm6973-4932429P05Rik 4:intergenic:Mettl21e-Gulp1 4:intergenic:6330403L08Rik/Pdgfa-Prkar1b 4:intron:Usp47 4:intergenic:Gm13009-Gm26001 4:intron:Atrnl1 4:intron:Dhx16 4:intergenic:Xylb-Acvr2b 4:intergenic:Gm24795-Gm8609 4:intergenic:Ppp1r18-Dhx16 4:intergenic:Gm11187-Fstl4 4:intron:Exoc3l 4:intergenic:Ifna4-Ifna1 4:intron:Ndufa9 3:intron:Pcdh9 4:intergenic:Sh3bp4-Agap1 4:intron:Plxna4 4:intergenic:Satb1-RP24-465O15.1 4:intergenic:Gpr149-Gm22433 4:intergenic:Gm25403-March1 4:intergenic:Gm14919-Gm14920 4:intergenic:Gulp1-Col3a1 4:intron:Fgf13 4:intron:Trnau1ap 4:intergenic:Hfe2-Gm15441 4:intergenic:Keap1-S1pr5 4:intron:Pebp4 4:intergenic:Fam196a-Dock1 3:intergenic:BC048507-Mtrr 3:intergenic:BC048507-Mtrr 3:intron:Efcab9 4:intron:Mkl1 4:intron:Smyd3 4:intergenic:St3gal1-Zfat 4:intergenic:Dapp1-Gm5105 4:intron:Frrs1 Off-target primers show all... show less...
432 / fw	`AATAAACTAAAGACTGGGCA GGG` `.................... ...` Enzymes: BmuI Cloning / PCR primers	46	66	50	45	71	86	0 - 0 - 6 - 36 - 367 0 - 0 - 1 - 8 - 13 409 off-targets	4:intron:Csmd2 4:intron:Bend7 4:intergenic:Gm5530-mmu-mir-6344 3:intron:Phf3 4:intergenic:Ankfn1-Pctp 3:intron:Ptprc 4:intron:Slc6a12 3:intergenic:6720401G13Rik-2610018G03Rik 4:intergenic:Rock1-Greb1l 4:intergenic:Gm13940-Katnbl1/Gm21985 3:intergenic:Adc-Ak2 4:intergenic:Tspan17-Gm16250 4:intron:Tns3 4:intron:Ttc27 4:intergenic:Ino80-Gm14208 4:intron:Ceacam20 4:intergenic:Tchh-Tchhl1 4:intergenic:Pdcl3-mmu-mir-6349 3:intergenic:Aldh1a1-Aldh1a7 4:intron:Zfp407 4:intergenic:Gm25969-A930011G23Rik 4:intron:Gm12472 4:intron:Xpo4 2:intergenic:Olfr678-Olfr679 4:intergenic:1700009N14Rik-Gm12385 4:intergenic:4833427F10Rik-Ier3 3:intron:Sugt1 4:intergenic:Gm13810-B230118H07Rik 4:intergenic:Guk1-2610507I01Rik 4:intergenic:Gm8349-Arhgef26 4:exon:4930578C19Rik 4:intergenic:Gm22989-Olfr722 4:intron:Klf8 3:intergenic:Klhl1-Gm25133 4:intron:Mapk12 4:intron:Plekhg6 4:intergenic:5830472F04Rik-Sag 4:intergenic:Olfr296-ps1-Olfr295 4:intergenic:Gm13337-Myo3a 4:intron:Rsc1a1/Ddi2 4:intron:Pnpla6 4:intron:Tsg101 4:intergenic:Gm17575-4930477E14Rik 4:intergenic:Mir181c-Gm26887 4:intergenic:Ccdc121-Dnah14 4:intron:1700021F05Rik 4:intron:Tnni3k 4:intron:Ubr5 4:intergenic:Tns3-Gm11990 4:intergenic:Rock1-Greb1l 4:intergenic:Rragc-Gm12916 3:exon:Gm20522/Gpank1 4:intergenic:Gm25096-Gm25634 4:intergenic:Gm25864-Kat6b 4:intergenic:Sgk3-Gm6195 4:intron:Itpa 4:intron:Tnfrsf21 4:intergenic:Gm7645-Stmn3 2:intergenic:Fhad1-Gm13037 4:intron:Iqch 3:intergenic:Gm8991-Gm6673 4:intron:Coro1c 3:intergenic:Gm26028-Gm23576 4:intron:Gm14005 4:intergenic:Gm13321-Pter 4:intron:Cntnap5a 4:intron:Prpf6 4:intron:Upf2 4:intron:Scp2 4:intergenic:Gm5506-Gm5839 4:intergenic:Gm26026-Gm5513 4:intron:Atad1 4:intron:Rpia 4:intron:Tmem243 4:intron:Nudcd1 4:intergenic:Agbl1-Gm23239 4:intergenic:Gm5555-Gpr125 4:intergenic:Slc12a9-Ephb4 4:intergenic:Gm14261/Sall4-Gm25214 4:intron:Bcl2l13 4:intergenic:Mir148a-Gm26677 4:intron:Prkacb 4:intron:Gabpb2 4:intergenic:1700058G18Rik-Kcnmb4 4:intron:Mmp17 4:intron:1700023E05Rik 4:intergenic:Lrfn2-Gm22978 4:intergenic:Lpin1-Ntsr2 4:intergenic:Gm23950-Gm11218 3:intron:Fbln2 4:intergenic:Plec-Gm25720 4:intergenic:Gm13071-Gm24381 4:intergenic:Ncoa7-Hey2 4:intron:Lsamp 4:intergenic:Cox7a2l-Kcng3 4:intron:Pde3a 4:intron:Melk 4:intron:Rab9 4:intergenic:Gm26559-Gm24471 4:intergenic:Slit3-Gm26070 4:intergenic:Ctif-1700034B16Rik 4:intergenic:AC158354.1-Gm4804 4:intron:Ptpn9 4:intergenic:Gpr1-Gm11608 4:intergenic:Gm23733-6530403H02Rik 4:intron:Lama2 4:intron:Lpcat3 4:intergenic:Gm12892-Gm12896 4:intergenic:Gm12893-Gm12895 4:intergenic:Ube2e3-Gm14466 4:intron:Wbscr25 4:intron:Ccdc79 4:intergenic:Gm26654-Pafah1b1-ps1 4:intergenic:Gm8775-Gm23976 4:intergenic:Ccdc130-Gm23626 4:intergenic:Klc2-Pacs1 4:intergenic:Csl-Kitl 3:intergenic:Samd5-Stxbp5 4:intergenic:Adra2a-Gpam 4:intron:Myo15 4:intergenic:Gm7665-Cdh2 3:intergenic:Morc1-Trat1 4:intergenic:Gm25253-Cpa6 4:intergenic:Gm23520-Gm12131 4:intergenic:Angpt1/Gm17473-Gm17473 4:intron:Dse 4:exon:Muc4 4:intron:Cds1 3:intergenic:Agrp-Fam65a 4:intergenic:Gm10152-Fgf3 4:intron:Ak7 4:intergenic:Gm23022-Rab28 4:intron:Magi2 4:intergenic:Gm11988-Gm11989 4:intergenic:Npm3-ps1-Gm5878 3:intergenic:Scn7a-Gm13600 3:intron:Mrpl10 3:intergenic:Ptma-Pde6d 4:intergenic:Gm5185-Gm24921 4:intergenic:Trim72-Gm22809 4:intergenic:Gm24680-4921530L21Rik 4:intergenic:Pik3c2b-Ppp1r15b/Gm26616 4:exon:Gm5097 4:exon:Gm8978 4:intron:Zyg11b 4:intergenic:Gm26028-Gm23576 4:intergenic:Gm25402-Dennd2a 4:intergenic:1700042O10Rik-Ddc 4:intergenic:Gpr6-Fig4 4:intron:Actn2 3:intron:Tef 4:intron:Ercc4 4:intergenic:Mapk1ip1l-Lgals3 4:intron:Myo9a 3:intergenic:Gm8604-MALAT1 4:intergenic:Zfp281-Gm19705 4:intergenic:Mir465-Gm14683 4:intergenic:Gm23267-Med30 4:intergenic:Tssc1-Gm23342 3:intergenic:Mzf1-Vmn1r85 4:intron:Msh5 4:intergenic:St3gal6-Gm813 4:intergenic:Trit1-Gm12923 4:intron:Igsf21 3:intergenic:Ccdc84-Foxr1 4:intron:4930480K23Rik 4:intergenic:Gm24601-Gm26501 4:intron:4933427D06Rik 4:intron:Cln3 4:intron:Fbxl2 4:intron:C530008M17Rik 4:intron:Slc44a5 4:intron:Etl4 4:intergenic:Mir466j-Gm20611 4:intron:Rasef 4:intron:Tprg 4:intergenic:Hdhd1a-Prr16 3:intergenic:Psmb7-ps2/Gm6787-Wdr13 4:intergenic:Gm23718-Gm14585 4:intergenic:Tbc1d5-Satb1 4:intergenic:Epha6-Gm9017/Epha6 4:intron:Atp6v1g3 4:intergenic:Gm11355-Prl 4:intron:Hdlbp 4:intergenic:Gm22554-Hs3st5 4:intergenic:Ttc32-Gm7099 4:exon:Tex19.1 4:intron:Shisa9 4:intron:Sil1 3:intron:Gltscr1 4:intergenic:Met-Capza2 4:intron:Fancc 4:intergenic:Arhgap25-Prokr1 4:intergenic:Mrpl45-Gpr179 4:intergenic:Ncam2-Gm23355 4:intergenic:Ctbp2-Tex36 4:exon:Zfp445 4:intron:Ptprm 4:intron:Ppig 4:intergenic:Gm10398-Gm26660 4:intron:Adora2b 3:intergenic:Mamdc2-1700028P14Rik 4:intron:Kif26b 4:intergenic:Gm12027-Gm23386 4:intergenic:Gm12249-Larp1 4:intron:2410127L17Rik 3:intron:Pcdh7 2:intron:Nfia 4:intergenic:Gm13852-Chchd3 4:intron:Phlpp2 4:intron:Ppapdc1a 4:intron:Pcsk5 3:intergenic:4930571K23Rik-Kdm8 4:intron:Lrp5 4:intergenic:4833412C05Rik-Igf1r 4:intergenic:Tcrg-C1-Tcrg-V3 4:intergenic:Trgv2-Gm16602 3:intron:Copa 4:intergenic:Bbs12-Gm12540 4:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:Sncaip-Snx2 4:intron:Gatm 4:intergenic:Top2a-Igfbp4 4:exon:Gm15859 4:intergenic:Gm22696-Fut8 4:intergenic:Spock3-Gm24236 3:intergenic:Rasgrf2-Msh3 4:intergenic:Fcrl5-Etv3 4:intergenic:Gm17619-Gm14270 4:intron:Mospd3 4:intergenic:Rngtt-mmu-mir-8118 2:intron:Nr1h4 4:intergenic:2900079G21Rik-AC113095.1 4:intron:Akap11 4:intergenic:4930405P13Rik-Scpep1 4:intergenic:Pcdh15-Gm6419 4:intergenic:Olfr680-ps1-Olfr681 4:intergenic:4932415D10Rik-Gm4924 4:intergenic:Rap2a-Gm17613 4:intergenic:Tgfbr2-Rbms3 4:intron:Cntn3 4:intron:Slc25a12 4:intergenic:Ctbp2-Tex36 4:intron:Rnf26 4:intron:Jarid2 4:intron:Ptprk 4:intergenic:Synpr-Gm15913 4:intergenic:Hao2-5730437C11Rik 4:intron:Pacrg 4:intron:8030443G20Rik 4:intergenic:Gm24512-6030443J06Rik 4:intergenic:4930451G09Rik-Sept12 4:intron:Eya4 4:intergenic:Mir1264-Mir1298 4:intergenic:Gabra6-Gabrb2 4:intron:Ptpn1 4:intergenic:Il31-Lrrc43 4:intergenic:Gm14020-Frmd5 4:intron:Fbxo42 4:intergenic:Gm5300-Tmem106b 4:intron:Xpr1 4:intron:Rcl1 4:intron:Grm7 4:intron:Cdhr2 4:intron:Npas3 4:intergenic:Rsf1-1700006D18Rik 4:intergenic:Gm26173-Tbc1d4 3:intergenic:Slc52a2-Adck5 4:intron:Stim1 4:intergenic:Iqgap1-Zscan2 4:intergenic:U6-Spry3 4:intergenic:Gm14314-Gm14292 4:intergenic:Asb9-Gm15216 4:intergenic:Igkv1-115-Igkv2-112 4:intergenic:Igkv2-109-Igkv16-104 4:intergenic:Slc22a30-Gm6425 4:intergenic:Gm25261-Gm831 4:intron:Dync1i1 4:intergenic:Esr2-Tex21 4:intergenic:Gm10398-Gm26660 4:intergenic:Wipi2-Slc29a4 4:intergenic:Mdfic-Tfec 4:intergenic:Otub1-Cox8a 4:intron:Hmcn2 4:intron:Sypl 4:intergenic:Gria3-Gria3/Gm22413 4:intron:1700101E01Rik 4:exon:Pcnxl2 4:intron:Map4k3 4:intron:Psmd9 4:intergenic:L3mbtl3-Gm8709 4:intron:Atrnl1 4:intron:Atp7b 3:intron:Sntb1 4:intergenic:Vmn2r-ps86-Vmn2r76 4:intron:Wdfy4 4:intergenic:Gm23777-Slc16a7 4:intron:Ttn 4:intergenic:Zbtb45-Trim28 4:intergenic:Lcorl-Gm7931 4:intergenic:Ccdc14-Mylk 4:intron:Ttc29 4:intergenic:Gm25717-Dyrk3 4:intergenic:Scaf8-Tiam2 4:intron:Ntm 4:intergenic:Gm9621-Gm24200 4:intergenic:Exoc6b-Gm7507 4:intron:Snx29 4:intergenic:Fam189b-Gm23269 4:intergenic:Zfp945-Vmn2r-ps128 4:intron:Homer2 4:intron:Snrk 4:intergenic:Gm16391-Gm17538 2:intergenic:Fggy-9530080O11Rik 4:intergenic:Cpa4-Cpa5 4:intergenic:Snx7-Gm26871 4:intergenic:Sgcz-Gm23128 4:intron:Ell 4:intergenic:Nudt10-Bmp15 4:intergenic:Slc16a10-Rpf2 4:intergenic:Gm22798-Cog6 4:intron:Atp10a 4:intergenic:Tapt1-Ldb2 3:intergenic:Got2-Gm22780 4:intron:Ttc21a 4:intergenic:MDGA2/Mdga2-Mdga2 4:intergenic:1700123K08Rik-Zfp68/Gm24082 4:intron:Xrn1 4:intergenic:Ppp6r3-Gm23940 4:intergenic:Gm22016-Cdc73 4:intron:Dmd 3:intron:Csf1 4:exon:Bptf 4:intron:Gphn 4:intergenic:Gm17655-Plcxd1 4:intergenic:Chi3l4-BC051070 3:intron:Shroom3 4:intergenic:Eef1e1-Gm22035 4:intergenic:Olfr1181-Olfr1182/Gm13757 4:intron:Bmpr1a 4:intron:Shisa9 4:intron:Aoah 4:intron:Cers6 4:intergenic:Fam173b-Tas2r119 4:intron:Cask 4:intron:Heg1 4:intergenic:Sntb1-Has2 4:intergenic:Pibf1-Klf5 4:intergenic:Pogk-Tada1 4:intron:Pinx1 4:intergenic:Olfr1180-Olfr1181 4:intergenic:mmu-mir-7685-Bpi 3:intron:9230104L09Rik 4:intergenic:Gm15530-Arhgap31 4:intergenic:F2rl1-F2r 2:intergenic:Akr1c21-Akr1e1 4:intergenic:Zfp655-Zscan25 4:intergenic:Gm25264-Gm25959 4:intron:Btbd3 4:intergenic:Ofcc1-Gm9979 4:intron:Rgs12 4:intron:Bpifb5 4:intergenic:Mir760-Bcar3 4:intron:Fbxw15 3:intergenic:Krtap11-1-Tiam1 3:intergenic:Gm5160-Gm6457 4:intron:Kcnh1 4:intergenic:Emc2-Tmem74 4:intergenic:Gm13976-3110099E03Rik 4:intron:Adk 4:intergenic:Bcl6-1110054M08Rik 4:intergenic:Gm25460-Gm5263 4:intron:Zc3h8 4:intergenic:Lrba-Gm26343 4:intergenic:Map9-Npy2r 4:intergenic:Gm26861-Gm23415 4:intergenic:Rpsa-ps10-Mir3963 4:intron:Il15 4:intergenic:Gm25598-BC002059 4:intergenic:Gm23980-Gm22810 4:intergenic:Gm24253-Gm12241 4:intron:Fbn2 4:intergenic:Gm24478-Gm14255 4:intergenic:Rcn1-4930527A07Rik 4:intron:Ptx4 4:intergenic:Adh6b-Adh6-ps1 4:intergenic:Gm11478-Vmp1 4:intron:Cttn 4:intergenic:Gm22104-Gm25550 4:intergenic:Gm8369-Ms4a6b 4:intron:Hc 4:intron:Pebp4 4:intergenic:Gm10313-Gm24669 4:intergenic:Gm25658-Atp2b1 4:intron:Slc6a6 4:intergenic:Gm25602-Tbc1d32 4:intron:Atp10d 4:intron:Rassf3 4:intron:Dst 4:intron:Prr5l 4:intron:1700112E06Rik 4:intergenic:Gm23903-Ank 4:intergenic:Gm22886-Klhl14 4:intergenic:Exoc4-Gm13854 4:intron:Scn1a 4:intron:Ccdc171 4:intergenic:Gm6214-Pdha2 4:intergenic:Rasgrf2-Msh3 4:intergenic:Atp1a1-Mab21l3 Off-target primers show all... show less...
436 / fw	`AACTAAAGACTGGGCAGGGG TGG` `.................... ...` Cloning / PCR primers	44	60	48	44	69	65	0 - 0 - 9 - 60 - 414 0 - 0 - 1 - 8 - 6 483 off-targets	2:intron:Gbp9 3:intergenic:RP24-105I10.3-Gm10679 3:intergenic:Obox5-Gm7235 3:intergenic:RP23-163B8.6-Gm4018 2:intergenic:Rpl21-ps5-Gm24508 3:intron:Plbd2 4:intron:Sipa1l3 2:intergenic:Gm5294-Pdgfa 4:intergenic:Large-Gm23255 4:intergenic:Gm15659-Gm8812 4:intron:Reln 4:intergenic:Gm18355-Gm4745 4:intergenic:Gm7308-Parp11 3:intron:Sulf2 4:intron:Kdm5d 4:intergenic:Dmrt3-Dmrt2 4:intergenic:Enox1-Gm10132 4:intergenic:Ighv1-54-Ighv1-55 4:intron:Cdc123 3:intergenic:Lgals4-Lgals7 3:intergenic:Cdh10-Gm25976 4:intergenic:Gm10451-Mir5135 4:intergenic:Gm25598-BC002059 4:intron:Kif3c 4:intron:Lef1 4:intergenic:A930011G23Rik-Gm16228 4:intron:Hecw1 3:intergenic:Nmur1-1700019O17Rik 3:intron:Ptpn11 4:intergenic:Erg-Ets2 3:intergenic:Ddx46-B230219D22Rik 3:intron:Npas3 4:intergenic:Gm25602-Tbc1d32 4:intron:Smyd3 4:intron:Ethe1 4:intron:Scaf4 4:intron:Ctnnd2 3:intron:Olfr411 3:intergenic:Pik3r1-Gm25808 4:intergenic:Smg7-Nmnat2 4:intron:Med13l 4:intron:Erc2 4:intergenic:Gm3890-Rpl39l 4:intron:Adra1b 4:intron:Mdn1 4:intergenic:P2ry1-B430305J03Rik/Rap2b 4:intergenic:Gm11523-Cdk5rap3 4:intron:Plekhh3 4:intergenic:Npbwr1-Rb1cc1 4:intergenic:Cpq-Gm24476 4:intergenic:Pabpc2-Yipf5 4:intron:Prdm15 4:intergenic:Mafb-Gm14221 4:intergenic:Mir1931-Cdk17 4:intron:Cdyl2 4:intergenic:Fam110c-6030469F06Rik 4:intergenic:4933406D12Rik-Gm14111 4:intergenic:Adarb2-Gm10357 4:intergenic:Map3k9-Pcnx 4:intergenic:Gm24298-4921513I03Rik 4:exon:Ttyh1/D030047H15Rik 4:intergenic:Gm15322-Gm15323 4:intron:Agbl4 4:intron:Vwc2l 4:intron:Zzef1 3:intron:Setdb1 4:intron:Tmed6 4:exon:Gm26772 4:intergenic:Tshz3-Zfp536 4:intergenic:9930038B18Rik-Laptm4a 4:intergenic:Gm9897-Fzd1 3:intergenic:Gm15755-Snap47 4:intergenic:Gm26005-Epcam 4:intergenic:Gm15327-Gm15325 4:intron:Fetub 4:intron:Spock1 4:intergenic:Dpf3-Dcaf4 4:intron:Dctn6 4:intergenic:Tdrd3-Rps3a2 4:intergenic:Gm1815-Zfp353-ps 4:intron:Akap13 4:intergenic:Gucy2d-Lrrc32 4:intergenic:Gm14236-Gm24327 4:intron:Mfsd7b 4:intergenic:Rpl19-ps4/AC122290.1-Gm281 4:intergenic:Gm6327-Ercc4 3:intergenic:Rpl12-ps1-n-R5s210 4:intergenic:Gm13685-Zc3h15 4:intron:Ythdf2 3:intron:Akap11 3:intergenic:Gm12706-Ror1 4:intergenic:Hs6st2-Gm14581 4:intergenic:Ptpn18-Prss39 4:intergenic:Snrnp48-Bmp6 4:intergenic:Ces5a-Gm26843 3:intergenic:Nr5a2-Gm23763 4:intergenic:Bicd1-Gm15785 4:intergenic:Tshb-Sycp1 4:intergenic:Gm11675-Gm11676 4:intergenic:Grb14-Cobll1 4:intron:Pga5 4:intergenic:Gm11499-Gm11500 4:exon:Alg13 4:intergenic:Ncor2-Scarb1 4:intron:Pard3b 4:exon:Akr1a1 4:intergenic:Lgals4-Lgals7 4:intergenic:Gm23337-Gm26081 4:intron:2310035C23Rik 4:intron:Zbed4 4:intergenic:Acyp2-Gm12099 4:intron:Arhgap42 3:exon:Usp46/1700094C10Rik 4:intergenic:Gm14576-Gm5385 4:intergenic:Spata22-Olfr20 3:intergenic:9230109A22Rik-Basp1 4:intron:Itga11 4:intergenic:Gm25322-Epb4.1l5 4:intergenic:Atp2b4-Optc 4:intergenic:Gm14249-1700101G07Rik/Gm11011 4:intron:Tef 4:intron:Wdfy4 4:intergenic:Gm25824-Gm26977 4:intron:Mcm9 4:intergenic:Gm20654-Ly6h 4:intergenic:Gm23411-Gm10548 4:intergenic:Vdr-Gm22202 4:intergenic:Gm12746-Ssbp3 3:intron:Hrk 4:intergenic:Lrig2-Gm26091 4:intergenic:Tmem126b-Dlg2 4:intergenic:Gm10660-Nat1 4:intron:Shroom2 4:intergenic:Rpl31-ps8-Tcea1-ps1 4:intergenic:2610028E06Rik-Csf3r 4:intergenic:Tet1-Gm26479 4:intron:Cnot6l 4:intron:Bag3 4:intergenic:Irf2-Enpp6 4:intergenic:Gm23315-Rprm 4:intron:Rpn2 4:intergenic:Cox7a2l-Kcng3 4:intron:Eps8l1 4:intergenic:Gm15401-Tcf7l1 4:intergenic:Gm14983-Diap2 4:intergenic:Urgcp/Mrps24-Urgcp 4:intergenic:Rbm28-Gm24303 4:intergenic:Gm12907-4933424M12Rik 4:intron:Clcn5 4:exon:Uap1l1 4:intron:Ephb2 4:intron:Rbm26 4:intron:Pafah1b1 4:intron:Aifm1 4:intergenic:Ier5-Mr1 4:intron:Fshr 4:intergenic:Gm11824-1700120G11Rik 4:intron:Smoc1 3:intergenic:Cbx7-Pdgfb 4:intron:Ralgapa2 4:intron:Syt16 4:intergenic:Dip2c-Gm26601 4:intron:Sidt1 4:intron:Tfeb 4:intergenic:Gm23986-Gm24929 4:intergenic:Acaa2-Gm24559 4:intergenic:Gm15950-Mpv17l 4:intergenic:Smok3a/Smok3b-Smok3a 4:intron:Smok3c 4:intron:Smok3b 4:intron:Col24a1 4:intron:Cdh2 4:intergenic:Rbm19-Gm26474 4:intron:Nutm1 3:intergenic:Slc12a3-Gm15889 4:intron:Slc25a33 4:intron:Slc35d2 3:intron:Ctnna1 4:intergenic:4933401P06Rik-Ppp2r2c 4:intron:Capn12 4:intergenic:Gm26468-Rpsa-ps10 4:intron:Rrbp1 4:intron:Arhgef10 4:intergenic:Abhd17b-Tmem2 4:intron:Arid1b 4:intergenic:4930527B05Rik-1700071K01Rik 4:intergenic:Cib4-Gm25666 4:intergenic:Alox12-Alox12e 4:intergenic:Gm12791-Gm12797 4:intron:Ctps2 4:intergenic:Gm23776-Anapc15-ps 3:intron:Ccdc55 4:intron:Ppp3cc 4:intron:Ppm1l 4:intron:Nrxn3 4:intron:Ldlrad2 4:intergenic:Caap1/Gm10306-Plaa 4:intergenic:Dusp7-Rpl29 4:intron:Slc8a2 4:intergenic:Gm15443-Gm23535 4:intron:Ppp6r3 4:intron:Grik2 4:intergenic:Tnfsf9-Cd70 4:intergenic:Phf3-Ptp4a1 4:intron:Rasgrf1 4:intergenic:Zdbf2-Gm11606 4:intron:Mast1 4:intergenic:Inpp4b-4930579O11Rik 4:exon:Arhgap1 4:intergenic:Gm22016-Cdc73 4:intergenic:Ccdc54-Gm9575 4:intergenic:Gm25004-Fggy 4:intergenic:Gm23985-Gm4915 4:intergenic:Gm26016-Gm15934 4:intergenic:Slc22a19-Slc22a26 4:intergenic:Lcn10-Obp2a 4:intergenic:Gm23041-Nav3 4:intergenic:Pcnx-n-R5s62 4:intron:Pbx3 4:intergenic:Abcf3-Vwa5b2 4:intron:Alkbh4 4:intergenic:Xcl1-Gm26042 4:intergenic:Wee1-Swap70 4:intron:Ocm 4:exon:Zfp523 4:intron:Phf16 3:intron:Cacna1c 4:intergenic:Rpl38-Ttyh2 4:intergenic:Gm21614-Gm23249 4:intergenic:Gm9423-Depdc1a 4:intron:1700007B14Rik 4:intron:Gla 4:intron:Secisbp2l 4:intergenic:Tmem184b-Csnk1e 4:intergenic:Pde4b-Gm24869 2:intergenic:Guk1-2610507I01Rik 3:intergenic:Msx2-Gm5449 3:intron:Otof 4:intergenic:Gm15208-Bcl11b 4:intron:Tmem56 4:intergenic:Trim72-Gm22809 4:intron:Tmem132a 4:intron:2010109A12Rik 3:intron:Lsamp 4:intergenic:Hdhd1a-Prr16 4:intron:Zfp407 4:intergenic:Tssc1-Gm23342 4:intergenic:Tnfsf13b-Myo16 4:intron:Nkd1 4:exon:E130309D14Rik 4:intergenic:Gm24948-Nova1 3:intergenic:Gm4791-Gm22286 4:intergenic:Zfp951-Abcg3 4:intron:Psmb1 4:intergenic:Pdha1-Gm15195 4:intergenic:Gm26259-Pitrm1 4:intron:Synpo 4:intron:Rap1gap2 3:intron:Mapkap1 4:intergenic:Grik3-2610028E06Rik 3:intergenic:Nav3-E2f7 4:intergenic:Ptprs-Znrf4 4:intergenic:Gm11502-Gm11506 4:intergenic:Syf2-Gm16225 4:intergenic:Gm23534-Gm22727 4:intron:Mroh9 4:exon:4933427G17Rik 4:intergenic:Fbf1-Acox1 4:intron:Scfd1 4:intron:A430093F15Rik 4:intergenic:Rpl30-ps8-Foxn3 4:intron:Ppp1r9a 4:intergenic:Mapk1ip1l-Lgals3 4:intergenic:Glrp1-Arl4c 4:intron:Psma6 3:intergenic:Cgn-Gm15263 4:intergenic:Rpl21-ps4-Gm3839 3:intron:4930441J16Rik 4:intergenic:Zfp579-Fiz1 4:intergenic:Cd44-Pdhx 4:intergenic:Selrc1-Fam159a 4:intergenic:Gm7645-Stmn3 4:intergenic:4631405K08Rik-Plxna2 4:intron:4933431K23Rik 4:intron:Kcnd1 4:intron:BC053393 4:intergenic:Gm9929-Dusp12 4:intergenic:Gm13506-Gm13454 4:intergenic:Gm11675-Gm11676 4:intergenic:Gm24104-Gm26058 4:intergenic:Vmn2r-ps119-Vmn2r-ps120 4:intergenic:D930016D06Rik-Gm8258 4:intron:Ctnna1 4:intergenic:Clnk-Gm25505 4:intergenic:Gm22460-Akr1c14 4:intron:Chst11 4:intergenic:Cartpt-Mccc2 4:intergenic:Spam1-Hyal5 2:intron:Bmpr1a 4:intron:Col4a6 4:intergenic:Sf3b2-Gal3st3 3:exon:Gm20654 4:intron:Ash1l 4:intron:Adcy2 4:intron:Ntrk1 3:intron:Ccdc11 4:exon:Cd72/Tesk1 4:intergenic:Gm13531-Gm13532 4:intron:Col24a1 4:intergenic:Nrg3-Gm10713 4:intergenic:Crbn-Gm22418 4:intergenic:RP24-338F2.1-Tac1 4:intron:Agtpbp1 4:intron:Pnoc 4:intron:Bend7 4:intergenic:Psors1c2-Cdsn 4:exon:Clasp1 4:intergenic:Gm25485-Gm12460 4:intergenic:Gm22308-Cbfa2t2-ps1 4:intergenic:Fam76b-Sesn3 4:intergenic:Cap1-Gm12891 4:intergenic:AC102758.1-Sox6 4:intergenic:4833412C05Rik-Igf1r 4:intergenic:Mir690-Mb21d2 4:intron:Cdc123 4:intergenic:Immp2l-Gm25497 4:intergenic:Metrnl-Gm12589 3:intergenic:Mthfs-AF529169 3:intron:Exoc4 3:intergenic:Col22a1/Gm16308-Gm16308 4:intron:Rgs9 4:intron:Kif4 4:intergenic:Gm22295-Rsrc1 4:intron:Bace2 4:intergenic:Alk-Gm24736 4:intergenic:Gm15686-Gm7518 4:intergenic:Gm25651-4933400A11Rik 2:intergenic:Dab1-Gm12719 4:intergenic:Gm24696-Myc 4:intron:Rabgap1l 4:intergenic:Gm23013-Ppp1r3a 3:intergenic:Gm25700-Gm24935 2:intron:Rdh16 2:intron:Rdh18-ps 4:intergenic:Olfm1-Gm13373 4:intergenic:Chst1-Gm13816 3:intergenic:Gm24070-Gm24994 3:intergenic:B230110C06Rik-Oxsm 4:intergenic:Gm15619/Nek11-Nek11 4:intergenic:Hspa8/Snord14c/Snord14d/Snord14e-Bsx 4:intergenic:Gm5423-Mcm9 3:intron:4930517O19Rik 4:intron:Smyd3 4:intergenic:Cd70-Tnfsf14 3:intergenic:Gm13780-Phf21a 4:intergenic:Gm26111-Gm14667 3:intergenic:Sub1-Zfr 4:intron:Camta1 4:intergenic:Gja1-Gm9795 4:intergenic:Gm22507-Trhde 4:intergenic:Foxi3-Gm23485 4:intron:9530036O11Rik 4:intergenic:Gm23644-Gm15442 4:intergenic:Fam155a-n-R5s95 2:intron:Ptprc 4:intergenic:Gm24784-Chl1 4:intron:Trim14 3:intron:Shroom3 3:intergenic:Olfr1200-Olfr1201 4:intron:Cask 3:intron:Slc6a9 4:intergenic:Gm25414-Gm26449 4:intergenic:Gm22016-Cdc73 3:intergenic:Gm13924-Gm13925 4:intergenic:Kcnma1-Gm6158 4:intergenic:Dbh-Sardh/Gm17071 4:intergenic:4631405K08Rik-Plxna2 4:intron:Hecw1 4:intergenic:Atrx-Magt1 4:intron:Tmem163 4:intron:Cyp4f40 3:intergenic:Tcf7l2-Gm22271 4:intergenic:Taf1b-Grhl1 4:intergenic:Efnb1-Gm14812 4:intergenic:Rnf144b-4931429P17Rik 4:intergenic:Banp-Gm22 3:intergenic:Gm26235-Gm25392 3:intergenic:Gm7168-Dact2 3:intergenic:Foxn3-Gm19898 4:intron:Ntrk2 4:intergenic:3100003L05Rik-Gm21957 4:intergenic:1700019E08Rik-Gm13465 4:intergenic:Vmn2r-ps114-Vmn2r96 4:intergenic:Gm5963-Sema5b 4:intergenic:RP23-131O4.2-Gm23470 4:exon:Samd3 4:intergenic:Gm15218-Gm8317 4:intergenic:Ifng-Dyrk2/4932442E05Rik 4:exon:Zfp688 4:intergenic:Gm14261/Sall4-Gm25214 4:exon:Mllt3 3:intergenic:Abhd4-Olfr49 4:intron:Lrrtm1 4:intron:Scaper 4:intergenic:Zfp652-Gm11526 4:intron:Fbxw8 4:intergenic:Itfg3-Luc7l 3:intergenic:Gm23848-Trps1 4:intergenic:Perp-Tnfaip3 4:intron:Slco2a1 4:intergenic:Gm12909-Gm12910 4:intron:Ophn1 4:intergenic:Gm10247-Grin2a 4:intergenic:E330017L17Rik-Spocd1 4:exon:Tarbp2 4:intergenic:Spn-Gm25735 4:intergenic:Cacna1c-Gm25905 4:intergenic:Gm23041-Nav3 4:intergenic:Lep-Gm25589 4:intron:Epc1 4:intergenic:Hexb-Enc1 4:intron:Cep128 4:intergenic:Tcerg1l-Mapk1ip1 4:intergenic:Gm9621-Gm24200 4:intergenic:Gm23180-Gm22977 4:exon:Mea1/Ppp2r5d 4:intron:Xpot 4:intergenic:Gm9800-Fhit 4:intergenic:Ccl1-Tmem132e 4:intergenic:9330198N18Rik-Gm14488 3:intron:Ttc21a 4:intergenic:0610025J13Rik-Gm12788 4:intergenic:Nav3-E2f7 4:intron:Med13l 4:intergenic:Tmem123-Birc2 4:intergenic:Ptma-ps1-Zfp180 4:intron:Chd9 4:exon:BC046401 4:intergenic:Polr1d-Gsx1 4:intron:Taok1 4:intergenic:Pacsin2-Ttll1 4:intergenic:Got2-Gm22780 4:intergenic:Gm1698-Gm24202 4:intron:Sft2d2 4:intergenic:Gm23848-Trps1 4:intron:Adamtsl3 4:intergenic:4833418N17Rik-Sh3gl3 4:intergenic:Vax1-Kcnk18 3:intergenic:Mthfr-ps1-Gm25756 3:intergenic:Gm7191-Gm23494 3:intron:Rasgrp1 4:intergenic:Mc2r-mmu-mir-6356 4:intergenic:Tspan18-Gm23380 4:intergenic:AL844604.1-Mpdz 4:intron:Astn2 4:intergenic:En1-B230209K01Rik 4:intron:Siglec1 4:intron:Dock1 4:intergenic:Tiam1-Sod1 4:intergenic:Gm26933-Tnfrsf11b 4:intergenic:1700017B05Rik-Gm5121 4:intergenic:RP23-175E16.1-RP24-225I21.1/Kirrel3 4:intron:1700017D01Rik 4:intergenic:Ttc32-Gm7099 4:intergenic:Gm6285-Gm22090 4:intron:Ltbp1 4:intergenic:Cyp2b19-Cyp2g1 4:intron:Cit 4:intergenic:Gm23986-Gm24929 4:intergenic:Triml1-Triml2 4:intergenic:Irx1-Rpl9-ps4 4:intron:Ctnna1 4:intron:Hs3st5 4:exon:Gm20508 4:intergenic:Gm17033-4933406K04Rik 4:intron:Alk 4:intergenic:Ctif-1700034B16Rik 4:intergenic:4930564D02Rik-1700095B22Rik 4:intergenic:Gm7931-Slit2 4:intergenic:Sqrdl-Gm13989 4:intron:Cacna2d3 4:intron:Ntrk3 4:intergenic:Cfhr3-Gm26271 Off-target primers show all... show less...
218 / fw	`GACAGAGGCAGGGATTTCTG AGG` `.................... ...` Enzymes: BshFI, BstDEI, LpnPI, Hpy188I, BstC8I Cloning / PCR primers	40	56	66	50	72	77	0 - 3 - 21 - 79 - 497 0 - 0 - 4 - 5 - 12 600 off-targets	More than 500 off-targets, showing 24 with score >1.0 1:intergenic:Rab10-Gm26520 3:intergenic:Bod1l-mmu-mir-6414 2:intron:Arhgef18 2:intron:4930593A02Rik 3:intron:Trim25 3:intergenic:Gm16039-Gm16055 3:intergenic:AC121988.1-Gm25740 4:intergenic:Folr4-Panx1 3:intron:Ttc28 3:intergenic:Gm15164-AU015836 3:exon:Chuk 4:intergenic:Trim8-Arl3 4:intergenic:Mib1-1010001N08Rik 2:intergenic:Eri1-Gm16793 2:intron:D10Bwg1379e 1:intron:Srpx 4:intergenic:Parvg-1810041L15Rik 4:intron:Tiam2 4:intron:Rnf2 4:intergenic:Gulp1-Col3a1 2:intergenic:Igkc-Rpia 2:intergenic:Gm15202-Gm26317 2:intergenic:Gm10155-Lat 1:intergenic:Spag16-Gm23422 Off-target primers show all... show less...
146 / rev	`CACCACGCCCAGCTCAAGTT AGG` `.................... ...` Enzymes: BshFI, LpnPI, SmoI Cloning / PCR primers	39	86	50	36	64	75	1 - 6 - 20 - 77 - 303 1 - 1 - 1 - 1 - 2 407 off-targets	4:intergenic:Atp2b4-Optc 3:intron:Prex2 1:intron:Steap3 4:intergenic:Gm13542-Ubac1 2:intergenic:Gm9381-Ndst4 4:intron:Suco 3:intergenic:Sppl2a-Ap4e1 4:intergenic:Gm14867-Gm25202 4:intergenic:Gucy1b2-Gm24625 0:intergenic:Gm11867-Gm11868 3:intergenic:Gm9157-Ftx 3:intergenic:Gm13187/Frmd4a-Frmd4a 3:intron:Thada 4:intergenic:Mir30a-Gm27028/Mir30c-2 4:intron:Specc1l 4:exon:Lats1 4:intergenic:Begain-Gm22234 4:exon:Scai 4:intergenic:Cep89-Slc7a9 4:intergenic:Gm12050-Gm12051 4:intron:D030068K23Rik 4:intron:Gm16168 2:intergenic:Gm14534-5730405O15Rik 1:intron:Myrfl 4:exon:Syn1 3:intron:Arid2 4:intron:Smc3 1:intron:Ankrd28 4:intron:Dgkk 4:intergenic:Gm23667-Gm26439 4:intron:Trim37 4:intergenic:mmu-mir-6378-Gm25442 3:intron:Mtmr3 3:intron:Ppp1r12b 4:intron:Coch 4:intergenic:Gm26327-Gm6851 4:intergenic:Mbtps2-Mbtps2/Yy2 2:intergenic:Zfp804b-Gm24525 4:intron:Prkd1 4:intergenic:Galnt9-Noc4l 3:intergenic:Gm13326-Gm13325 4:intergenic:En1-B230209K01Rik 2:intron:Rtn4rl1 2:intron:Trp53bp1 4:intron:Dpp10 4:intron:Trim67 4:intergenic:Rbm14/Gm21992-Ccs 4:intergenic:Gm24120-Nr2f2 4:intergenic:Fcer2a-Clec4g/Gm9814 4:intergenic:Rnf182-Cd83 3:intron:Gak 3:intron:Elf4 3:intergenic:Add2-Tgfa 3:intergenic:Gm266-Eif5 4:intergenic:Oxgr1-Mbnl2 2:intergenic:Gm11268-Gm11269 4:exon:Cyp20a1 4:intergenic:Gm26704-Baalc 3:intron:Casp3 3:intron:Nbr1 4:intron:Pou6f2 4:intron:Rims1 3:intergenic:Gm23055-Med18 4:intron:Eef2k 4:intergenic:Gm24861-Chodl 4:intergenic:Gabrg2-Gabra1 4:intergenic:Xkr5-Defb40 3:exon:Arpc1b/Pdap1 3:intron:Glyr1 4:intron:Bmper 1:intergenic:Gm16685-Gm24614 2:intergenic:H2afy2-Mir5108 4:exon:Lysmd4 4:intron:Exoc6b 4:exon:Cacna1e 4:intron:Ank2 4:intron:Hmcn1 4:intron:Fam76b 3:intron:Poldip3 4:intergenic:Mbd2-Dcc 2:intron:Lmod3 4:intergenic:Gm11840-Gm11842 3:intron:Impdh1 4:intergenic:Dcakd/Nmt1-Nmt1 4:intergenic:Sh3gl2-Gm12680 3:intergenic:Gm22459-Gm25041 4:intergenic:Atxn7l1-4930556N08Rik/Atxn7l1 3:intron:Zc3hc1 4:intergenic:Furin-Gm15544 4:intergenic:Gm24839-Tmem132c 1:intergenic:Fer-Pja2 4:exon:Ptchd4 4:intron:Mgmt 4:intergenic:Gm21614-Gm23249 2:intergenic:Lamc1-Gm8818 3:intron:Smim8 4:intergenic:Epha4-Gm26263 2:intergenic:Trmt10c-Gm24047 3:intergenic:Gm25216-Pyroxd2 4:intron:Gabbr2 2:intergenic:Sall1-Gm6625 4:intergenic:Gm8192-4930555O08Rik 4:intron:Clint1 3:intron:Pgc 4:intergenic:Tigd4-Tmem154 4:intron:Brd4 4:intron:Ctnna1 3:intron:Spata21 4:intron:Neto1 4:intergenic:Etv6-Bcl2l14 3:intergenic:Nrp2-Ino80d 4:intergenic:Zscan4d-Gm26248 4:intergenic:Zscan4c-Zscan4d 4:intergenic:Vmn1r71-Zscan4b 4:intergenic:Gm11931-Gabrr2 4:intron:Ros1 4:intergenic:Mfsd1-Iqcj 3:intron:Txn2 3:intergenic:Ifng-Dyrk2/4932442E05Rik 4:intron:Sufu 3:intron:Pkd2l2 2:intergenic:Cubn-Trdmt1 4:intron:Deptor 3:intron:Pde6b 4:intergenic:RP23-2C22.1-RP24-456G3.3 4:intergenic:Grb2-Gm11703 4:intron:Gpkow 4:intron:Abl1 2:intergenic:Gm12152-Pwwp2a 3:intergenic:Gm14775-Il1rapl1 3:intron:Dcp1a 4:intron:Shroom4 4:intergenic:Wbp4/Elf1-Elf1 4:intron:Acsf2 3:intergenic:Efr3b-Dnajc27 4:intron:Dym 4:intergenic:Src-Gm14279 3:intergenic:Gm9103-Rps4x 4:intergenic:Hmbs-Vps11 4:intron:Esr1 4:intergenic:Gm10384-Zfp706 3:intergenic:Gab3-Dkc1 4:intergenic:Snrpd3-Fam211b 4:intergenic:Gm23740-Gm12059 4:intergenic:Wbp5-Ngfrap1 4:intergenic:Nol11-Gm11718 4:intergenic:Gm24692-Sptssa 4:intron:Ttc39c 4:intron:Ccdc41 4:intron:Dab2 4:intergenic:Gm8730-Cdh5 4:intergenic:Gm26904/Gltscr1l-Gltscr1l 4:intron:Sgcz 1:intergenic:Dennd5a-Tmem41b 4:intergenic:Pdgfb-Rpl3 4:exon:Fam189b 4:intergenic:Gm11344-Cmah 4:intergenic:Gm16331-Dach1 3:intergenic:C2cd5-Etnk1 3:intergenic:2310042E22Rik-Ephb3 4:intergenic:4932435O22Rik-Gm11689 4:intergenic:Gm23131-Lphn2 3:intron:Slc28a3 4:intergenic:n-R5s209-Gm7846 4:intron:Ckap5 4:intergenic:Atp6v0e2-Lrrc61 3:intron:Pxk 4:intron:Asap1 4:intron:Cenpj 3:intergenic:Ppp1r3c-Tnks2 4:intergenic:Dym-Gm8807 3:intron:Acvr1b 4:intron:Pdia3 4:intergenic:Utp23-Rad21 2:intron:Ubxn11 4:intergenic:Gm23670-Mrps28 4:intergenic:Gm23061-Ostf1 4:intergenic:Ptk7-Gm26904 4:intergenic:Gm25923-Gm25564 4:intergenic:Srr-Srr/Smg6 4:intergenic:Gm14293-Gm14294 4:intergenic:Gm24477-Trp53rk 4:intron:Cnot6l 4:intergenic:RP23-433F5.5-RP23-433F5.6 2:intron:Phka1 4:intergenic:Imp3-Snupn 2:intron:Arhgef3 4:intergenic:Prdm2-Gm22039 4:intron:Nipal2 4:exon:Kctd13 3:intergenic:Gm26241-Gm26089 4:intergenic:Ptgis-Gm11473 4:intergenic:Terf2-C630050I24Rik 4:intergenic:Gm23590-Sp1 4:intron:Phkg2 4:intron:Tfg 4:intron:Lrba 4:intron:Sec24a 4:intergenic:Gm12413-Sh3gl2 3:intergenic:Esyt2-Ncapg2 4:intergenic:Gm10800-Gm13809 4:intergenic:Scaf11-Slc38a1 4:intergenic:Gm22344-Cbr3 4:intron:Creld1 4:intergenic:Strbp-Crb2 4:intron:Sgcb 4:intergenic:Nup133-Abcb10 3:intergenic:Tfap2c-Gm22773 3:intergenic:Rph3al-1700016K19Rik 4:intergenic:AC154801.1-BC040587 4:intergenic:Gap43-4932412D23Rik 4:intergenic:Exoc3l2-Bloc1s3 3:intergenic:Pip5k1b-Tmem252 4:intergenic:Gm26885-Pim1/Gm26885 4:exon:Lrrc29 4:intron:Itsn1 4:intron:Slc25a21 4:intergenic:Fjx1-Pamr1 4:intron:Vps53 4:intron:Rbm47 4:intergenic:Zswim4-D8Ertd738e 3:intergenic:Tbce-Ggps1 4:intron:Brd1 4:intergenic:Tcte2/Mllt4-Mllt4 4:intron:Gm20614 4:intergenic:Gata1-Glod5 3:intergenic:Tceb3-Gm24362 4:intron:Pik3ap1 4:intron:Wdr46 4:intergenic:Ivd-Bahd1 4:exon:Prrc2c 4:intergenic:Tsacc/Rhbg-Tsacc 4:intergenic:Plekhf2-Ndufaf6 4:intergenic:Gpr98-9330111N05Rik 4:intergenic:Sept1-Zfp553 4:intergenic:Gm12370-Mir876 3:intron:Nav1 3:intron:Eapp 3:intron:Zmym2 4:intergenic:Zfp873-AU041133 4:intron:Ddx19b 4:intron:Xylb 4:intergenic:Gm25459-n-R5s170 4:intergenic:Canx-Gm12196 4:intergenic:Dym-Gm20544 4:intron:Hunk 4:intergenic:4930589P08Rik-Camta1 3:intron:Best2 4:intergenic:Commd4-Trcg1 4:intron:Mfsd1 4:intergenic:Rad51ap2-Gm23781 4:intergenic:Rfx4-Gm24226 4:intergenic:D430036J16Rik-Gm9531 4:intergenic:Prex1-Gm24477 4:intergenic:H2-Q1-Gm8741 4:intron:Ubxn2a 4:intron:Kmt2a 4:intergenic:Gm5250-Prdm14 4:intron:Pdk3 4:intron:Golph3l 4:intergenic:Ccnd1-Tpcn2 4:intergenic:Gm14320-Cebpb/Tmem189 4:intergenic:Prkar2b-Pik3cg 4:intergenic:Gm14672-Gm14668 4:intergenic:Slco1a4-Slco1a1 4:intron:Anks1 4:intron:Rassf4 4:intron:Exosc10 4:intron:Plekhg1 4:intergenic:Gm13053-Tmem51os1/Tmem51 4:intron:Dapk1 4:intergenic:Gm23728-Foxb1/B230323A14Rik 4:intergenic:Slc25a44-Sema4a 4:intergenic:Slc2a3-Gm4482 3:intergenic:Arhgap39-Zfp251 4:intergenic:Gm7069-Gm10649 4:intergenic:Gm10874-Zfp157 4:intron:Fgd1 4:intron:Megf11 4:intergenic:Gm12819-Gm12821 4:intron:Ralgps2 4:intergenic:1700112E06Rik-Gm5670 4:intergenic:Etl4-Gm13362/Etl4 4:intergenic:Robo3-Msantd2 4:intergenic:Gm5423-Mcm9 4:intron:Kctd10 4:intergenic:Gm14261/Sall4-Gm25214 4:intergenic:Dagla-Syt7/Gm26859 4:intergenic:n-R5s82-Pknox2 4:intron:Afap1 4:intergenic:Olfr49-Oxa1l 4:intron:Cep112 2:intergenic:Gm5380-Gm7129 2:intron:Acox1 2:intron:Eml5 2:intron:Macf1 3:intergenic:Gm9621-Gm24200 3:intergenic:Malt1-Zfp532 3:intergenic:Gm25008-Capn11 3:intergenic:Pik3c2b-Ppp1r15b/Gm26616 3:intergenic:Ppap2b-Gm12722 3:intron:Tns1 3:intergenic:Nr6a1-Gm13591 3:intergenic:Gm12700-Cachd1 3:intergenic:Gm25537-Gm24122 3:intergenic:Fancg-Pigo 3:intergenic:mmu-mir-6411-Gm22945 3:intergenic:Gm10800-Gm13809 3:intron:Crx 3:intron:Cth 3:intergenic:Psip1-Gm11414 3:intron:Epb4.1l4b 3:intron:Mtbp 3:intergenic:Hnf4a-Ttpal 3:intergenic:Jmy-Bhmt 3:intergenic:BC080695-Gm13058 3:intergenic:Gm26296-Col14a1 3:intergenic:Slfn10-ps-Slfn2 3:intron:Map7d1 4:intergenic:Ucp1-Elmod2 4:intergenic:Gm25342-Rps15a-ps1 4:intron:Clcn5 4:intron:Dhtkd1 4:intron:Ptbp3 4:exon:Nasp 4:intergenic:Olfr372-Olfr373 4:intron:2500002B13Rik 4:intron:Rbm17 4:intron:Ankrd6 4:intergenic:Rpia-Eif2ak3 4:intergenic:Gm22137/Gm7541-Tmem186 4:intergenic:5730409E04Rik-Gm12933 4:intergenic:Alppl2-Alpi 4:intergenic:Gm13100-Gm13106 4:intron:Igdcc4 4:intergenic:Tmem260-Gm23469 4:intergenic:Gm14825-4932442L08Rik 4:intergenic:Gm25985-Gm6404 4:intergenic:Cog7-Gga2 4:intron:Asxl2 4:intergenic:Gm23349-4930488B22Rik 4:intergenic:Rab12-Themis3 4:intergenic:Gast-Hap1 4:intergenic:Cacng1-Cacng4 4:intron:Lmtk2 4:intron:Adamts7 4:intron:Stoml3 4:intron:Adamts13 4:intergenic:Gm22024-Gm26721 4:intron:Wbscr25 4:exon:Ryr1 4:intergenic:Ddit4-Anapc16 4:intron:Slit1 4:intron:Lmo7 4:intron:Ift74 4:intron:Otof 4:intergenic:Trdv2-2-Trdv4 4:intergenic:Tekt4-Sstr5 4:intergenic:Polr3b-Rfx4 4:intergenic:Xrcc5-March4 4:intergenic:Mki67ip-Clasp1 4:intergenic:Znhit3-Car4 4:intergenic:Sgpp1-Syne2 4:intron:Csf3r 4:intergenic:Chn2-9530036M11Rik 4:intergenic:Gm23379-Zranb2 4:intron:Numb 4:intergenic:Gm14621-Hs6st2 4:intergenic:Ankrd55-Il6st 4:intergenic:Gm16137-Grhl2 4:intron:Ddx26b 4:intron:Pxk 4:intergenic:Rtn4rl2-Gm24332 4:intergenic:Rybp-Gm23234 4:intron:Acp2 4:intergenic:Cnksr3-Ulbp1 4:intron:Epb4.1l4b 4:exon:Mettl13 4:intergenic:Uaca-Gm10655 4:intergenic:Gm12784-Kctd15 4:intergenic:Gm2302-Gm9268 4:intron:Mtbp 4:intergenic:Slc30a5-Gm10257 4:intron:Pdpn 4:intron:Diap3 4:intergenic:Mnat1-Gm23910 4:intron:Il1rap 4:intron:Slc25a32 4:intergenic:4930526D03Rik-Oprl1 4:intron:Gas6 4:intergenic:Rmi1-Slc28a3 4:intron:AI846148 4:intron:Phospho2 4:intergenic:Sec61g-Akt2-ps 4:intron:Sema5a 4:intergenic:Gm12059-1700061J23Rik 4:intergenic:Rhbdf2-Cygb 4:intergenic:Gm11187-Fstl4 4:intron:Cotl1 4:intron:Kif1c 4:intergenic:Icam5-Zglp1 4:intergenic:Gm24610-Tmem180 4:intergenic:AI429214-Gm25126 4:intron:Acyp1 4:intergenic:B930078G14Rik-Fam155a 4:intergenic:Abcb5-Itgb8 4:intergenic:Crk-Ywhae Off-target primers show all... show less...
297 / rev	`CTTTGGGAAATGTTTTTTTT TGG` `.................... ...` Not with U6/U3 Inefficient Cloning / PCR primers	32	54	13	21	70	81	0 - 1 - 12 - 89 - 625 0 - 1 - 0 - 6 - 13 727 off-targets	More than 500 off-targets, showing 23 with score >1.0 2:intergenic:Gzmb-Atp12a 3:intron:Psd3 2:exon:Pdgfb 2:intron:Uty 3:intron:Dab2ip 4:intergenic:Gm11986-U7 3:intron:Scarb1 3:intergenic:1700042G15Rik-Gm12537 3:intergenic:Dnmbp-n-R5s21 2:intron:Wdr31 3:intron:Ctps2 3:intron:Fmo2 2:intron:Gm973 4:intron:Ldhb 4:intergenic:Gm4784-Gm4991 4:intergenic:Tmem168-B630005N14Rik 3:intron:Gfod2 3:intron:Pkd1l3 3:intron:Tvp23b 4:intergenic:Gm16203-Sgcz 4:intergenic:Gm23681-Peli1 1:intergenic:Gm16421-Gm14946 2:intergenic:Gm24773-Ncam2 Off-target primers show all... show less...
143 / fw	`CAGGTTTATACCACCATGAC TGG` `.................... ...` Enzymes: BshFI, Bse1I, NlaIII Cloning / PCR primers	12	28	53	36	78	76	0 - 5 - 39 - 286 - 1767 0 - 1 - 4 - 35 - 16 2097 off-targets	More than 500 off-targets, showing 23 with score >2.0 2:exon:Ric8 2:intergenic:Mup-ps18-Mup-ps19 3:intergenic:AC125059.1-Pacs1 2:intergenic:Usp17lb-Usp17la 2:intron:A430010J10Rik 2:intergenic:Ccl25-Cers4 2:intron:D030068K23Rik 2:intergenic:Gm8806-Gm6954 2:intergenic:Gm6083-Ube3c 2:intergenic:4933415F23Rik-Gm24176 2:intergenic:Bmp4-Gm15221 2:intron:4933427D14Rik 2:exon:Rin3 2:intergenic:Gm23532-Slc34a2 1:intergenic:Perp-Tnfaip3 1:intergenic:Gm22827-Gm22181 2:intergenic:Gm23326-Thy1 2:intron:Arntl2 2:intron:Efhb 2:intergenic:Gm25949/A330041J22Rik-A330041J22Rik 1:intron:Apoc2 1:intergenic:Arap3-Pcdh1 1:intergenic:Slc25a25-Naif1/Slc25a25 Off-target primers show all... show less...
167 / fw	`CTAACTTGAGCTGGGCGTGG TGG` `.................... ...` Enzymes: ApaLI, BaeGI, HpyCH4V, Hpy166II, Bsp1286I, Alw21I Cloning / PCR primers	7	16	61	68	52	55	0 - 1 - 27 - 264 - 1310 0 - 1 - 18 - 125 - 379 1602 off-targets	More than 500 off-targets, showing 22 with score >3.0 2:intergenic:C230029F24Rik-PCGEM1 1:intron:Clvs1 2:intergenic:Gm11867-Gm11868 2:intron:Trdn 2:intron:Ankrd28 2:intron:Rad51b 2:intergenic:2700049A03Rik-Dact1 2:intron:Gm26862 2:intergenic:Rps19-ps12-Gm23083 2:intron:Rrn3 2:intron:Dcp1b 2:intergenic:Aurkc-Zfp954 2:intergenic:Gm23644-Gm15442 2:intron:Ern1 2:intron:Ubxn11 2:intergenic:Gm22025-Mid2 2:intergenic:Gm23752-Gm24815 2:intergenic:Mal2-Nov 2:intergenic:Gm13555-Slc4a10 2:intron:Armc8 2:intron:Camk2b 2:intron:1700007B14Rik Off-target primers show all... show less...
227 / fw	`AGGGATTTCTGAGGCCAGCC TGG` `.................... ...` Inefficient Enzymes: Hpy166II, LpnPI, BstNI, StyD4I, FblI, BstC8I Cloning / PCR primers	3	20	44	45	62	86	0 - 12 - 195 - 305 - 840 0 - 11 - 179 - 164 - 167 1352 off-targets	More than 500 off-targets, showing 12 with score >10.0 1:intergenic:Macf1-mmu-mir-6398 1:intergenic:Gm24143-Ube2g1 1:intergenic:Gm14514-Efhc2 1:intron:Trim33 1:intron:Arhgef9 1:intron:Plekhg1 1:intron:Ptp4a2 1:intron:Slc7a6 1:intron:Stk24 1:intron:Rnf44 1:intron:Rnf11 1:intergenic:Gm22883-Ppm1e Off-target primers show all... show less...
51 / rev	`AGGTAATTCCAGTACAGCCA GGG` `.................... ...` Enzymes: Tru1I, LpnPI, BseDI, BstNI, StyD4I	0	0	65	44	74	76	Repeat
52 / rev	`GAGGTAATTCCAGTACAGCC AGG` `.................... ...` Inefficient Enzymes: Tru1I, LpnPI, BseDI, BstNI, StyD4I	0	0	55	39	68	82	Repeat
54 / fw	`AGAGTTTCTCTGTTTAACCC TGG` `.................... ...` Enzymes: StyD4I, LpnPI, BseDI, BstNI	0	0	56	24	74	79	Repeat
63 / fw	`CTGTTTAACCCTGGCTGTAC TGG` `.................... ...` Enzymes: TatI, Bse1I, Csp6I, MluCI	0	0	33	41	55	55	Repeat
71 / rev	`AAGGCCAGCCTGGTCTATAG AGG` `.................... ...` Enzymes: MluCI, BfmI	0	0	52	50	64	87	Repeat
81 / rev	`TTCTGAGTTTAAGGCCAGCC TGG` `.................... ...` Inefficient Enzymes: StyD4I, LpnPI, BstNI, BstC8I	0	0	41	38	65	91	Repeat
83 / fw	`TGGAATTACCTCTATAGACC AGG` `.................... ...` Enzymes: StyD4I, LpnPI, BstNI, BstC8I	0	0	64	31	67	83	Repeat
87 / fw	`ATTACCTCTATAGACCAGGC TGG` `.................... ...` Enzymes: BshFI, StyD4I, LpnPI, BstNI, BstC8I	0	0	60	27	73	79	Repeat
176 / rev	`CTCCCAAGTGCTGGGATTAA AGG` `.................... ...` Enzymes: Tru1I, BstC8I	0	0	20	58	69	81	Repeat
184 / rev	`GCCTCTGTCTCCCAAGTGCT GGG` `.................... ...` Enzymes: BslI, Tru1I, Van91I	0	0	42	48	76	87	Repeat
185 / rev	`TGCCTCTGTCTCCCAAGTGC TGG` `.................... ...` Enzymes: BslI, Van91I	0	0	29	21	72	81	Repeat
193 / fw	`ACGCCTTTAATCCCAGCACT TGG` `.................... ...` Enzymes: BslI, Van91I	0	0	47	44	73	79	Repeat
194 / fw	`CGCCTTTAATCCCAGCACTT GGG` `.................... ...` Inefficient Enzymes: BslI, Van91I	0	0	56	31	69	83	Repeat
203 / fw	`TCCCAGCACTTGGGAGACAG AGG` `.................... ...`	0	0	64	67	61	80	Repeat
207 / fw	`AGCACTTGGGAGACAGAGGC AGG` `.................... ...`	0	0	53	38	65	63	Repeat
208 / fw	`GCACTTGGGAGACAGAGGCA GGG` `.................... ...`	0	0	69	56	66	72	Repeat
221 / rev	`ACTCACTTTGTAGACCAGGC TGG` `.................... ...` Enzymes: BshFI, MnlI, LpnPI, BstNI, StyD4I, BstC8I	0	0	61	17	69	82	Repeat
225 / rev	`TGGAACTCACTTTGTAGACC AGG` `.................... ...` Enzymes: Hpy166II, LpnPI, BstNI, StyD4I, FblI, BstC8I	0	0	52	29	61	73	Repeat
245 / rev	`CTGTGTAACCCTGGCTGTCC TGG` `.................... ...` Enzymes: StyD4I, LpnPI, PfoI, BstNI	0	0	35	49	63	57	Repeat
247 / fw	`TGGTCTACAAAGTGAGTTCC AGG` `.................... ...` Inefficient Enzymes: StyD4I, LpnPI, PfoI, BstNI	0	0	36	41	58	72	Repeat
254 / rev	`AGGGTTTCTCTGTGTAACCC TGG` `.................... ...` Enzymes: StyD4I, LpnPI, MaeIII, BstNI, BseDI	0	0	59	39	71	75	Repeat
256 / fw	`AAGTGAGTTCCAGGACAGCC AGG` `.................... ...` Inefficient Enzymes: StyD4I, LpnPI, MaeIII, BstNI, BseDI	0	0	52	43	67	75	Repeat
257 / fw	`AGTGAGTTCCAGGACAGCCA GGG` `.................... ...` Enzymes: StyD4I, LpnPI, MaeIII, BstNI, BseDI	0	0	67	42	71	80	Repeat
273 / rev	`TTTGTTGTTTTTTTGAGACA GGG` `.................... ...` Not with U6/U3	0	0	51	39	68	80	Repeat
274 / rev	`TTTTGTTGTTTTTTTGAGAC AGG` `.................... ...` Not with U6/U3	0	0	31	32	69	85	Repeat

Download as Excel tables: Guides / Guides, all scores / Off-targets / Saturating mutagenesis assistant
Tab-sep format: Guides / Off-targets

New Query

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License