CRISPOR

Mus musculus (mm10), chr14:54895129-54895478, forward genomic strand

Your input sequence is 350 bp long. It contains 27 possible guide sequences.
Shown below are their PAM sites and the expected cleavage position located -3bp 5' of the PAM site.
Click on a match for the PAM NGG below to show its 20 bp-long guide sequence. (Need help? Look at the CRISPOR manual)
Colors green, yellow and red indicate high, medium and low specificity of the PAM's guide sequence in the genome.

Position  0         10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       
Variants  ..............................................................................................................................................................................................................................................................................................................................................................
Sequence  TTTGTTAGACACAAGCCGTTTTGGTGAAGAGATAGTCTTGTGCCTTCCTTTGTCTCATTTATAAATGTGTTTCTCTTTGTTCTTAGTTTGTCTGTACCCTTCTTGGGGCGATTATCAGCTGGCATTTGACCTTCAAGTCTTAACACTTTTCAGTTGGAGACCCTTTAGTTAGGACTTAGGAACTGACTGGTGGCTGTAAGGGAGGGAGTTCTTTAAAAATGTTAGATTTCAGTCCATTCTGTTTTGTTTGCTTTGAGGCATTTCAGCTAAAGCTGAATAGAGACTCGACTTGAAAAACGTAAAATCTTAACACCGTTTTTTATGGTCTTTTAAGAGGTAGTTTCTCTAAT
                         CCG---                     CCT---                                                CCC---              −−−TGG       CCT---                −−−TGG   CCC---        −−−AGG    −−−TGG     −−−AGG                                CCA---             −−−AGG                                                      CCG---             −−−AGG
                            −−−TGG                      CCT---                                             CCT---                                                          CCT---                    −−−TGG   −−−GGG                                                                                                                     −−−TGG
                                                                                                              −−−TGG                                                             −−−AGG                          −−−AGG
                                                                                                               −−−GGG                                                                                             −−−GGG
                                                                                                                −−−GGG

Download for: SerialCloner (free) - ApE (free) - GenomeCompiler - Benchling - SnapGene - Geneious - Vector NTI - LaserGene - Genbank - FASTA

Predicted guide sequences for PAMs

Ranked by default from highest to lowest specificity score (Hsu et al., Nat Biot 2013). Click on a column title to rank by a score.
If you use this website, please cite our paper in NAR 2018. Too much information? Look at the CRISPOR manual.

Download as Excel tables: Guides / Guides, all scores / Off-targets / Saturating mutagenesis assistant

Position/ Strand	Guide Sequence + PAM + Restriction Enzymes + Variants Only G- Only GG- Only A-	MIT Specificity Score	CFD Spec. score $The CFD specificity score, inspired like guidescan.com, behaves like the MIT specificity score, but it is based on the more accurate CFD off-target model, from <a href='http://www.nature.com/nbt/journal/v34/n2/full/nbt.3437.html'>Doench 2016</a>, which is also used by Crispor to rank the off-targets. The CFD specificity score correlates better than the MIT score with the total off-target cleavage fraction of a guide, see <a target=_blank href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731277/'>Tycko et al, Nat Comm 2019</a> and also the <a target=_blank href='/manual/#faq'>CRISPOR manual</a>.$	Predicted Efficiency Show all scores		Outcome		Off-targets for 0-1-2-3-4 mismatches + next to PAM	Genome Browser links to matches sorted by CFD off-target score exons only chr14 only
				Doench '16	Mor.-Mateos	Out-of-Frame	Lindel
22 / fw	`TTGTTAGACACAAGCCGTTT TGG` `.................... ...` Inefficient Enzymes: AsuHPI Cloning / PCR primers	91	94	35	50	65	77	0 - 0 - 0 - 5 - 75 0 - 0 - 0 - 0 - 0 80 off-targets	4:intron:Phka1 4:intergenic:Scube1-Mpped1 4:intergenic:Gm23228-Fbxo4 4:intron:Pde4d 4:intergenic:Gm26164-Gm10283 4:intron:Wdfy1 4:intron:Mylk 4:intergenic:G3bp1-Gm22884/Gm12238 4:intergenic:Gm8806-Gm6954 3:intron:Cnnm2 4:intergenic:Gm8584-Gm9637 4:intergenic:Gm24280-Rbm26/Gm17066 4:intergenic:Gm24039-4930461C15Rik 4:intron:Ttll11 4:intron:Khdrbs1 4:intron:Hmgxb3 4:intergenic:AI429214-Gm25126 4:intergenic:Fam198b-Gm6706 4:intergenic:Msr1-Fgf20 4:intergenic:Gm11923-Gm11925 4:intron:Abca12 4:intergenic:Crispld1-Crisp4 3:intergenic:Pea15b-Gm22011 4:intergenic:Pdgfd-Ddi1 4:intergenic:Cyp3a41b-Cyp3a41a 4:intergenic:Gm26015-Gm25599 4:intergenic:Drosha-Gm24664 4:intergenic:Olfr981-Olfr982 3:intergenic:Gm16305-Dennd1b 4:intron:Anxa4 4:intergenic:Gm6317-Gm25383 4:intergenic:Gm3756-Arhgap32 4:intergenic:Slc38a9-Ppap2a 4:intergenic:Cask-Gm5381 4:intron:Tmem178 4:intron:Antxr1 4:intergenic:Tas2r125-Tas2r129 4:intergenic:Hnrnpa1l2-ps-Pcsk1 4:intergenic:Mitf-Gm15531 4:exon:Nfia 4:intergenic:Hpse2-Gm22135 4:intron:Armc9 4:intergenic:Gm22978-Gm20540 4:intron:Dcc 4:intergenic:Gm5622-Gm17186 4:intergenic:Mrgprx1-Mrgprb5 4:intergenic:Prps1l3-Gm11025 4:intergenic:Gm12387-Gm26087 4:intron:Slc15a2 4:intergenic:Gm24126-Gm26124 4:intron:2610206C17Rik 4:intron:Hmgcll1 4:intergenic:Gm12701-Ror1 4:intergenic:Ddx18-Gm15394 4:intergenic:Gm14899-Tex16 4:intergenic:Gm6446-Gm15057 4:intergenic:Fau-ps1-Macrod2 4:intergenic:Tpbg-Ube2cbp 4:intron:Ezh2 4:intergenic:Gm23708-G6pd2 4:intergenic:Plcl1-Hsfy2 4:intergenic:Tekt1-mmu-mir-6338 4:intergenic:Gm13497-Gm13499 3:intron:Nuf2 4:intergenic:Gm22689-Gm9742 4:intron:Lsmem1 4:intergenic:Ctnna3-Gm22829 4:intergenic:Xlr4c-Xlr3c 4:intergenic:Xlr3b-Xlr4b 4:intergenic:Xlr3e-ps-Xlr4e-ps 3:intron:Mgat5 4:intergenic:Gm25388-Galnt5 4:intron:Hibch 4:intron:Il6ra 4:intergenic:Zbtb38/E030011O05Rik-Acpl2 4:intergenic:Gm26349-Mrps30 4:intergenic:Stim2-Gm24235 4:intron:Pcdh9 4:intergenic:Vmn2r65-Vmn2r66 4:intergenic:Gm14135-Gm14132 Off-target primers show all... show less...
120 / fw	`TTCTTGGGGCGATTATCAGC TGG` `.................... ...` Enzymes: AluBI, MspA1I, PvuII, BstC8I Cloning / PCR primers	88	95	51	31	78	72	0 - 0 - 0 - 3 - 49 0 - 0 - 0 - 0 - 0 52 off-targets	4:exon:Tyrp1 4:intron:Dnah7b 4:intergenic:Cxxc1-Mbd1 3:intron:Sorcs2 4:intron:Slc7a1 4:intergenic:4930449E01Rik-mmu-mir-6390 4:intergenic:Slc22a15-Nhlh2 4:intergenic:Ctif-Gm10532 4:intergenic:Erbb2ip-Nln 4:intron:Eaf2 4:exon:Olfr652 4:intergenic:Gm12050-Gm12051 4:intergenic:1500002O10Rik-Gm16292 4:intron:Erbb4 4:intron:Dock9 4:intron:Dgki 4:intron:Vmn2r60 4:intergenic:9430093N23Rik-Gm14250 4:intergenic:Gm20516-Gm21947 4:intron:Mylip 4:intergenic:Wdfy3-Arhgap24 4:intron:Anks1b 4:intergenic:Gm23892-Gm25382 3:intergenic:Serp2-Lacc1 4:intergenic:Mir3962-Gm23885 4:intergenic:Polr1a-St3gal5 4:exon:Utrn 4:intergenic:Gm14448-Gm14450 4:intergenic:Gm15553-Hspa13 4:intergenic:Gm23937-Gm22944 4:intergenic:Fbxw7-Mir466k 4:intergenic:Stc2-Bod1 4:intergenic:Gm22557-Brdt 4:intergenic:Gm25693-Gm12129 4:intergenic:Gm23827-Papolg 4:intergenic:AY702102-A630075F10Rik 4:intergenic:Gm14109-Gm14107 4:intron:Kcnq1 4:intergenic:mmu-mir-6344-Irs1/Gm9747 4:intergenic:Cry1-AC100386.1 4:intergenic:Fut8-Gm24070 4:intergenic:Slc14a2-Gm6133 3:intergenic:Gm24720-Tshz1 4:intergenic:Rarres1-Gm25919 4:intergenic:Sdc1-9930038B18Rik 4:intergenic:Gm24503-Ccrn4l 4:intergenic:Gm24880-Gm10172 4:intergenic:Gm11412-n-R5s187 4:intergenic:1700085B03Rik-Tmem132c 4:intergenic:Grem2-Rgs7 4:intergenic:Gm23670-Mrps28 4:intergenic:Eif3s6-ps1-Gm11995 Off-target primers show all... show less...
171 / fw	`CAGTTGGAGACCCTTTAGTT AGG` `.................... ...` Cloning / PCR primers	88	95	39	46	81	82	0 - 0 - 1 - 5 - 83 0 - 0 - 0 - 0 - 0 89 off-targets	4:exon:Rac2 4:intron:N4bp1 4:intergenic:Lrrc7-Gm9423 4:intergenic:Gm26177-Gm25602 4:intron:Dcakd 4:intergenic:Olfr1410-Olfr12 4:intron:Ctnna2 4:intergenic:Mcf2-Atp11c 3:intergenic:Gm14210-Plcb4 4:intergenic:AL844604.1-Mpdz 4:intron:Aass 4:intergenic:A630031M04Rik-1700112K13Rik 4:intron:Pbrm1 4:intron:Cobll1 4:intron:9430020K01Rik 2:intron:Fis1 4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Gm4219-E330013P04Rik 4:intergenic:Gm11990-Pkd1l1 4:intergenic:Dgkb-Etv1 4:intergenic:Rpl21-ps5-Gm24508 4:intron:RP23-264N13.2 4:intergenic:Dlx2-Gm1631 4:intergenic:Gm5432-Lpin1 4:intergenic:Oser1-Gdap1l1 4:intergenic:Lypd6-Mmadhc 4:intergenic:Celf2-Gm24340 4:intron:Fam172a 4:intergenic:Slco3a1-Gm22326 4:intron:Prr5l 4:intergenic:Gm8183-Tmem135 4:intergenic:Eml4-Cox7a2l 4:intergenic:Cdh19-Dsel 4:intergenic:Acat2-Gm15946 3:intergenic:Gm26844-Gm4812 4:intron:Zp2 4:intron:4930556M19Rik 4:intergenic:Casp8-Trak2 4:intron:Gm15338 4:intron:Rbfox3 4:intergenic:Sgcz-Gm23128 4:intron:Inpp5a 4:intron:Wbscr17 4:intergenic:Mir298-Nespos 4:intron:Tinag 4:intron:Tnpo1 4:intergenic:Gm25255-Csmd1 4:intergenic:Ica1-Nxph1 4:intron:Hcfc2 4:intron:Nfx1 4:intergenic:Gm9790-Gm25876 4:intergenic:Gm24127-Pcdh9 4:intergenic:Abcg2-Herc3 4:intergenic:Gm24737-Lhfpl2 4:intergenic:Gm12414-Bnc2 4:intergenic:D230002A01Rik-Gm24254 4:intron:Thsd7a 4:intergenic:Gm6977-Gm44 4:intron:Nsmce2 4:intron:Atxn7 4:intergenic:Gm23644-Gm15442 4:exon:Ubald1 3:intron:Ext1 4:intron:Cdk6 4:intergenic:Gm11783-Gm11786 4:intergenic:Gap-Sepp1 4:intergenic:Vmn1r-ps6-Samd9l 4:intergenic:Gap-Gm17177 4:intergenic:Gap-Gm7346 4:intergenic:Gm21719-Gm20830 3:intergenic:Jazf1-Gm25818 4:intron:Vwa9 4:intron:Magi1 4:intergenic:Ttc39d-Gemin6 3:intron:Ppp1r13b 4:intron:Tpd52l1 4:intron:Fat4 4:intergenic:Ftl2/Mir692-2a-Grik3 4:intergenic:Bean1-Gm10631 4:intergenic:Gm5641-Gm26678 4:intergenic:Olfr976-Olfr978 4:intergenic:Reg4-Hmgcs2 4:intron:Dock1 4:intergenic:Casp12-Pdgfd 4:intron:Rfwd3 4:intergenic:Irx1-Rpl9-ps4 4:intergenic:Trim29-Pvrl1 4:intergenic:Vezf1-Cuedc1 4:intergenic:Gm25923-Gm25564 Off-target primers show all... show less...
97 / rev	`GCTGATAATCGCCCCAAGAA GGG` `.................... ...` Enzymes: Csp6I, BslI Cloning / PCR primers	87	94	55	49	65	81	0 - 0 - 0 - 2 - 66 0 - 0 - 0 - 0 - 0 68 off-targets	4:intergenic:Gm25392-Gm24238 4:intron:Agbl4 4:intergenic:Gm13018-Prdm2 4:intergenic:Dmrta1-Gm12629 4:intron:F830016B08Rik 4:intergenic:Chst11-Slc41a2 4:intergenic:Gm24633-Gm25568 4:intergenic:Gm25722-Gm10051 4:intron:N4bp1 4:intergenic:Sept7-Eepd1 4:intergenic:Gm23013-Ppp1r3a 4:exon:Gm26793/Fgf15 4:intergenic:Mthfs-AF529169 4:intron:Pacsin2 4:intron:Kcnq1 4:intergenic:Gm11904-Gm11902 4:intron:Lmx1b 4:intergenic:Tmem69-Gpbp1l1 4:intergenic:Gm8429-Rpl21-ps4 4:intergenic:4930569F06Rik-Suds3 4:intron:Gm12258 4:intergenic:Lipc-Aqp9 4:intergenic:Tspan3-C230081A13Rik 3:intron:Sox6 4:intergenic:Gm22802-Slitrk5 4:intergenic:Gm23526-Grik4 4:intergenic:Gm5742-Gm22223 4:intron:Vmn2r60 4:intergenic:Gm25828-Nhsl2 4:intergenic:Gap-Gm16088 4:intergenic:Ppp1r2-ps7-Gm26131 4:intergenic:Neurod4-Tespa1 4:intergenic:Gm23937-Gm22944 4:intergenic:Cbln2-Gm5096 4:intergenic:Pole4-Hk2 4:intergenic:Gm14448-Gm14450 4:intron:Antxr1 3:intergenic:Olfr704-Olfr705 4:intron:Specc1l 4:intergenic:Gm25693-Gm12129 4:intergenic:Stc2-Bod1 4:intron:Rock1 4:intergenic:Gm23827-Papolg 4:intron:Cntnap5b 4:intergenic:Gm22557-Brdt 4:intergenic:n-R5s209-Gm7846 4:intergenic:CU207330.1-Fam171a1 4:intergenic:Gm5742-Gm22223 4:intergenic:D3Ertd751e-Gm25714 4:intron:Hhat 4:intergenic:Gm23477-Gm10780 4:intron:C230081A13Rik 4:intron:Zfp423 4:intergenic:1700025G04Rik-Tsen15 4:intergenic:H19/H19_1/Mir675/H19_2-Igf2 4:intergenic:9430093N23Rik-Gm14250 4:intergenic:Gm20516-Gm21947 4:intergenic:Gm24963-Gm10742 4:intergenic:Gm16565/Cd247-Gm23402 4:intergenic:Gm14055-Gm14064 4:intergenic:Oxgr1-Mbnl2 4:intron:Rps6ka1 4:exon:Kdsr 4:intergenic:Slc22a15-Nhlh2 4:intron:Gldc 4:intergenic:Hmgb1-ps9-Pik3r1 4:intergenic:Dyrk1b-Eid2 4:intron:Nfasc Off-target primers show all... show less...
98 / rev	`AGCTGATAATCGCCCCAAGA AGG` `.................... ...` Enzymes: Csp6I, BslI Cloning / PCR primers	87	93	56	41	62	80	0 - 0 - 0 - 6 - 52 0 - 0 - 0 - 0 - 0 58 off-targets	4:exon:Fbxw11 4:intergenic:BC048507-Mtrr 3:intron:Zfp423 4:intergenic:Gm2231-Gm2913 4:intergenic:Hgf-Gm8991 4:intergenic:Sema3c-Cd36 4:intergenic:Htra1-Dmbt1 4:intergenic:L3mbtl3-Gm8709 4:intergenic:Cxxc1-Mbd1 3:intergenic:Gm11984-Adcy1 4:exon:Slc5a8 4:intron:Adcy2 4:intergenic:Ppapdc1a-Wdr11 4:intergenic:5033426O07Rik-Gm26815 4:intergenic:Gm10275-Cenpw 4:intergenic:Rps18-ps1-Gm11231 4:intron:Dnm3 4:exon:Npy1r 4:intergenic:Capn9-2310022B05Rik 4:intergenic:Id2-Gm22527 4:exon:Gm26793/Fgf15 4:intron:N4bp1 4:intergenic:Nup160-Fnbp4 3:intron:Gas7 4:intergenic:Ceacam13-Ceacam12 4:intergenic:Gm12738-Zcchc11 4:intron:6330403A02Rik 4:intergenic:Gm25505-Gm25377 4:exon:Nrxn3 3:exon:Gm12758/4931406P16Rik 4:intron:Mkl1 4:intergenic:Gm23735-Fndc3a 4:intergenic:Med30-Ext1 4:intron:2900079G21Rik 3:intergenic:2010300C02Rik-Tsga10 4:intron:Parva 4:intergenic:Gm7481-Prkd1 4:intergenic:Rps2-ps6-Tsc22d1 3:intron:Mgat5b 4:intron:1700016K19Rik 4:intron:Dnah7b 4:intron:Tex14 4:intergenic:Msx1-Stx18 4:intron:Gramd1b 4:intron:Dgki 4:intergenic:Pole4-Hk2 4:intergenic:A330048O09Rik-Zfp169 4:intron:Greb1 4:intron:Gldc 4:intergenic:Gm27039-Adcyap1 4:intergenic:Ret-Bms1 4:exon:P4ha2 4:intergenic:Gm26146-Gm8226 4:intergenic:Olfr372-Olfr373 4:intron:Spock1 4:intergenic:Trps1-Eif3h 4:intron:Trappc9 4:intron:Htr7 Off-target primers show all... show less...
161 / rev	`GTTCCTAAGTCCTAACTAAA GGG` `.................... ...` Enzymes: BspTNI, BcoDI Cloning / PCR primers	86	90	44	43	70	85	0 - 0 - 0 - 8 - 82 0 - 0 - 0 - 0 - 0 90 off-targets	4:intergenic:Depdc5-Ywhah 4:intergenic:Gm13810-B230118H07Rik 4:intergenic:Cgnl1-RP23-79K5.1 4:intergenic:Gm10226-Zfp760 4:intergenic:Olfr532-Olfr533 4:intergenic:Gm11824-1700120G11Rik 4:intergenic:Hs3st3a1-Gm24275 4:intron:Ttc28 4:exon:Farp1 4:intron:Bcl6b 3:intergenic:Zfp640-Gm17404 3:intergenic:AC164297.1-Zfp640 3:intergenic:Gm6917-Gm6910 4:intergenic:Gm12297-Gm12298 4:intergenic:Synpo2l-Sec24c 4:intergenic:Ddx39b-Ddx39b/mmu-mir-8094 4:intergenic:Bcar1-Cfdp1 4:intergenic:Gm23643-Gm24632 4:intron:Chd1l 4:intergenic:Gm9992-Gm25872 4:intergenic:4930550L24Rik-Fgf13 4:intergenic:Sec61b-NAMA_1 4:intron:Gm14461 4:intergenic:Anapc10-Hhip 4:intergenic:Fli1-RP24-88B13.1 4:intron:Sptan1 3:intron:Vwa8 4:intergenic:Cdh10-Gm25976 4:intergenic:Mamdc2-1700028P14Rik 4:intron:Syt7 4:intergenic:Gm25656-Cntn6 4:intergenic:Gm8587-Gm10436 3:intergenic:Gm23534-Gm22727 4:intergenic:Suco-4930558K02Rik 3:intron:Tmem70 4:intron:Ank3 4:intron:Rpe 4:intergenic:Tusc1-Gm12649 4:intron:Hcn1 4:intron:Iqsec2 3:intergenic:Gm22521-Gm25702 4:intergenic:Lphn3-Mir1187 4:intron:Astn1 4:intergenic:Gm8707-Sorl1 4:intergenic:Rwdd1-Trappc3l 4:intergenic:Gm22845-Nt5c1b 4:intergenic:Psma6-Nfkbia 4:intergenic:Gm22756-Gm8941 4:intron:Ppp1r9a 4:intergenic:Gm14944-Gm14942 4:intergenic:4930432M17Rik-F3 4:intergenic:Gm11782-Gm11781 4:intergenic:Maf/Gm15655-Dynlrb2 4:intron:Gm16838 4:intergenic:Sorcs3-AC116451.1 4:intergenic:Gm26126-Gm11114 3:intergenic:4930595M18Rik-Gm8822 4:intron:Tep1 4:intergenic:Nrg3-Gm20642 4:intron:Kcnc2 4:intergenic:Gm17655-Plcxd1 4:intergenic:Zic4-Gm26101 4:intergenic:Gzmk-BC067074 4:intergenic:Nav3-E2f7 4:intergenic:AC121988.1-Gm25740 4:intergenic:Vma21-Gm1141 4:intergenic:Tyr-Grm5 4:intron:Ostc 4:intron:Sox5 4:intergenic:Pard3-mmu-mir-21c 4:intergenic:Zfp260-Zfp382 4:intron:Kcnt2 4:intergenic:Atp6v1e1-Bcl2l13 4:intergenic:Cnksr3-Ulbp1 4:intron:Gm12381 4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Fgfr1op-Ccr6 4:intron:Smoc1 4:intergenic:Gm8730-Cdh5 4:intron:Spats2l 4:intron:Anks1b 4:intergenic:Mir2136-Gm25904 4:intron:Foxn3 4:intron:Hexim2 4:intergenic:Gm13812-Gm13810 4:intergenic:Gm13810-B230118H07Rik 4:intergenic:Pax3-Sgpp2 4:intron:6430628N08Rik 4:intergenic:Rtp4-Gm6640 4:intergenic:Gm25384-Cxcr4 Off-target primers show all... show less...
323 / fw	`AATCTTAACACCGTTTTTTA TGG` `.................... ...` Not with U6/U3 Inefficient Cloning / PCR primers	83	86	10	10	71	80	0 - 0 - 1 - 14 - 94 0 - 0 - 0 - 4 - 0 109 off-targets	4:intergenic:Btbd11-Pwp1 4:intergenic:Gm22673-Gm23059 4:intergenic:Gm11351-Gm11352 3:intron:Prickle1 4:intron:Dennd1b 4:intergenic:Csgalnact1-Ints10 4:intergenic:Cit-Gm14508 2:intergenic:Nov-Gm7577 4:intergenic:Tbc1d22b-Rnf8/RNF8 4:intergenic:B530045E10Rik-Gad1-ps 4:intron:Elovl6 4:intergenic:Smad2-Skor2 4:intergenic:5730420D15Rik-Mrpl42 4:intron:Gucy1a2 4:intergenic:Gm26825/Gm15459-Gm23838 4:intron:Rapgef5 4:intergenic:Zfp326-Gm25672 4:intergenic:Gm23126-Mrps9 4:intron:Creb3l1 4:intron:Sdccag8 4:intergenic:Gm1821-Gm15217 4:intergenic:Gm26638-Gm26807/Tmem178b 4:intergenic:Gm14657-Gm8260 4:intergenic:l7Rn6-Eed 4:intron:Slit3 3:intergenic:Gm6776-Hpse2 3:intergenic:Gm26321-Gm13597 3:intergenic:Gm22308-Cbfa2t2-ps1 3:intergenic:RP24-376I11.1-RP23-402M18.1 4:intergenic:Snx19-Adamts15 4:intron:Nedd1 3:intergenic:Nox3-Gm23186 3:intron:Frk 4:intergenic:Mitf-Gm15531 4:intergenic:Gm25414-Gm26449 4:intron:Malt1 3:intergenic:Gjd2/A530058N18Rik-A530058N18Rik 4:intergenic:Gm15867-Syt14 4:intron:Synj2 4:intergenic:Spanxn4-Gm7763 4:intron:Abhd12 4:intergenic:Rad21-Aard 4:intergenic:4930505H01Rik-Gm23769 4:intergenic:Lrtm1-Cacna2d3 4:intergenic:Gm22169-Gm22962 4:intron:Lphn3 4:exon:Crb1 4:intergenic:Sema3d-Gm23243 4:intron:Efna5 4:intergenic:Gm25297-Dlk1 4:intergenic:Gm26905-Galntl6 3:intergenic:Fgf9-Gm25614 4:intergenic:Gm26480-Gm6863 4:intergenic:Gm10068-Als2cr12 4:intron:Gm12239 4:intron:Dlg2 4:intergenic:Runx3-Clic4 3:intergenic:Tmprss15-Rps19-ps12 4:intergenic:Gm13203-Casz1 3:intron:Col12a1 4:intergenic:1700010H22Rik-1700007G11Rik 4:intergenic:Dock10-Nyap2 4:intergenic:Gm6771-Ptchd4 4:intergenic:Specc1-Adora2b 4:intron:Ppwd1 4:intron:Vat1l 4:intron:RP23-302F9.4 4:intergenic:Neil3-Vegfc 4:intron:Cbl 3:intergenic:A230028O05Rik-Tprg 4:intergenic:Ncam2-Gm23355 4:intergenic:Nlrp4d-Vmn1r67 4:intergenic:Pinc-Gm25329 4:intergenic:Grk1-Tmem255b 4:intergenic:Gm14672-Gm14668 4:intergenic:Fat3-Gm5611 4:intergenic:Gm20091-Gm8601 4:intron:Lpp 4:intergenic:Gm11369-Uqcrfs1 4:intergenic:Gm23884-Gm25684 4:intergenic:Gm22016-Cdc73 4:intron:Egfl6 4:intron:Gm14643 4:intergenic:Gm7367-A230006K03Rik 4:intron:1200014J11Rik 3:intergenic:Gm14823-Gm14855 4:exon:Homer1 4:intron:Gm8773 4:intergenic:Gm6985-Pcyt1b 4:intergenic:Gm1698-Gm24202 4:intron:Snx13 4:intron:Dst 4:intron:Adam17 4:intergenic:Gm11263-Gm11409 4:intergenic:Gm25798-Tcerg1l 4:intergenic:Gm22544-Gm22509 4:intergenic:Mrpl19-Eva1a 4:intergenic:Gm25935-Gm22593 3:intron:Gria4 4:intergenic:Kctd16-Rps19-ps13 4:intergenic:Gm23892-Gm25382 4:intergenic:Mki67-Gm9341 4:intergenic:Crispld2-Zdhhc7 4:intron:Ccdc91 4:intergenic:Pappa2/1600012P17Rik-Gm4953 4:intergenic:Gm14268-5031425F14Rik 4:intergenic:Pcdh18-Slc7a11 4:intergenic:Gm22939-Cntnap2 4:intron:Ptprz1 Off-target primers show all... show less...
162 / rev	`AGTTCCTAAGTCCTAACTAA AGG` `.................... ...` Enzymes: BspTNI, BcoDI Cloning / PCR primers	80	91	49	22	67	80	0 - 0 - 0 - 16 - 88 0 - 0 - 0 - 1 - 1 104 off-targets	4:intron:Hdac9 3:intron:Gm3985 4:intergenic:Mcf2-Atp11c 3:intergenic:Gm6917-Gm6910 3:intergenic:Zfp640-Gm17404 3:intergenic:AC164297.1-Zfp640 4:intergenic:Nkain2-Trdn 4:intergenic:Gm26923-Gm25143 4:intergenic:Nsmce2-Trib1 4:intron:Pitpnc1 4:intergenic:Defa-ps9-Defa-ps1 4:intergenic:Gm15313-Defa17 4:intergenic:Defa-ps5-Defa-ps6 4:intergenic:Gm15305-Defa-ps18 4:intergenic:Defa-ps8-Defa3 4:intergenic:Gm15300-Gm15299 4:intergenic:Gm15311-Gm6696 4:intergenic:Cetn3-Gm24295 4:intergenic:Bcar1-Cfdp1 4:intron:Itga9 4:intergenic:Gm22520-Grk5 4:intron:3110009E18Rik 4:intergenic:Gm12290-F930015N05Rik/Hs3st3a1 4:intron:Tmprss11e 4:intron:D10Bwg1379e 4:intron:Tenm1 3:intergenic:Gm10710-Gm22342 4:intergenic:Gm7847-Gm23674 4:intergenic:Gm8116-Col12a1 4:intergenic:Gm12297-Gm12298 4:intergenic:Dnah5-Mir3964 4:intron:Nsf 4:intron:Arl15 4:intron:Ranbp17 4:intergenic:Apob-1110057K04Rik 4:intergenic:Sphkap-Gm25754 4:intergenic:Speer6-ps1-Gm16505 4:intron:Pcdh7 4:intergenic:Baalc-Gm26704 4:intergenic:Gpr156-Gsk3b 4:intergenic:Gm14823-Gm14855 4:intergenic:A630014C17Rik-Vdac1 4:intron:Sncaip 4:intergenic:Gm5530-mmu-mir-6344 3:intron:Camta1 4:intron:Bmp7 4:intron:Abcc5 4:intergenic:Gm25268-Ide 3:intergenic:Gm11259-Gm11256 4:intergenic:Dnajc22-Spats2 3:intergenic:Fgfr1op-Ccr6 4:intron:Prkg1 3:intron:Tmem70 4:intergenic:Slc22a3-Slc22a2 4:intergenic:4933430M04Rik-Gm25507 4:intergenic:Pmvk-Kcnn3 4:intergenic:Atf6-Gm25235 4:intergenic:Gm23959-Gm12016 4:intron:Dram1 4:intron:1700034G24Rik 4:intergenic:Htr1a-Dph3b-ps 4:intergenic:Gm9341-Gm24444 4:intron:Tcf4 4:intergenic:Gm6471-Th 4:intron:Dnajc18 4:intergenic:Gm24080-Gm22104 3:intron:Sptan1 4:intron:Skap1 4:intron:Gm6498 4:intergenic:Ccdc25-Scara3 4:intron:Lpin1 4:intergenic:Gm24679-Gm22219 4:intergenic:Cgnl1-RP23-79K5.1 3:intergenic:Nrf1-Mir182 4:exon:Ppm1g 3:intergenic:Ppp1r2-ps9-Gm14519 4:intergenic:Hrh4-Gm22251 4:intron:Cped1 4:intergenic:Gm12023-Gm23093 4:intergenic:Gng2-ps1-Gm11905 4:intron:Ica1 3:intergenic:Uchl5-Rgs2 4:intergenic:Arhgap26-Arhgap26/9630014M24Rik 3:intron:Luzp2 4:intergenic:F11r-B930036N10Rik/Gm24871/Alyref2 3:intron:Map2k6 3:intergenic:Nedd4l-Mir122a 4:intergenic:Lrriq3-Gm23038 4:intergenic:1700019D03Rik-Mstn 4:intron:Dmrt1 4:intergenic:Gm11499-Gm11500 4:intergenic:Gm23477-Gm10780 4:intergenic:Ralgps2-Tex35 4:exon:Commd1 4:intron:Zfp947 4:intergenic:Enah-Srp9 4:intergenic:Gm23437-Gm24818 4:intergenic:Ptpla-4921530L18Rik 4:intron:Gm1989 4:intergenic:Gbp3-Gbp2b 4:intergenic:Gm24847-Gm26164 4:intergenic:Gm12029-Spred2 4:intergenic:AC121988.1-Gm25740 4:intron:2210404O09Rik Off-target primers show all... show less...
178 / fw	`AGACCCTTTAGTTAGGACTT AGG` `.................... ...` Inefficient Enzymes: BstDEI Cloning / PCR primers	78	87	52	22	75	88	0 - 0 - 3 - 4 - 91 0 - 0 - 0 - 0 - 2 98 off-targets	2:intergenic:Fli1-RP24-88B13.1 2:intergenic:Gm26923-Gm25143 4:intergenic:Rps18-ps1-Gm11231 4:intron:8430419L09Rik 3:intergenic:Fbln2-Wnt7a 3:exon:Pigw 4:intergenic:Gm14746-Gm14748 4:intron:Atp2b1 4:intron:Stat3 4:intergenic:AC164297.1-Zfp640 4:intergenic:Zfp640-Gm17404 4:intergenic:Gm6917-Gm6910 4:intron:Pard3 4:intergenic:Commd1/RP23-242C19.7-Zrsr1/Commd1 4:intergenic:Gm24728-Lmo4 4:intron:Shank2 4:intron:Txnrd1 4:intron:Diap3 4:intergenic:Dnajc22-Spats2 4:intergenic:Gm24587-Dcn 4:intron:Gm15514 4:intergenic:Ttll11-Ndufa8 4:intron:Fbxl2 4:exon:Rapgef5 4:intergenic:Gm22246-Flrt2 4:intergenic:Efcab8-Gm14494 4:intron:Efhd1 4:intergenic:Gm24374-Sash1 4:intergenic:Tdgf1-Lrrc2 4:intron:Sptan1 4:intergenic:Cdh13-Gm22999 4:intergenic:Mapk8-Ptpn20 4:intergenic:Gm7722-Gm14609 4:intergenic:Gm11779-Gm11782 4:intron:Smyd3 4:intergenic:Gm23649-Lclat1 4:intron:Tep1 4:intergenic:Gzmk-BC067074 4:intergenic:Gm22267-Tmem229a 4:intergenic:Irx1-Rpl9-ps4 4:intron:Sesn1 4:intergenic:Slc10a7-Lsm6 4:intron:Upf2 4:intergenic:Gm5944-Gm14903 4:intergenic:Ythdf3-Gm26485 4:intron:Aar2 4:intron:Smim5 4:intergenic:Atp11c-Mir505 4:intergenic:Gm11680-BC006965 4:intergenic:Gm24999-Gm25000 4:intron:Zbtb17 4:intergenic:Sectm1a-Gm11792 4:intron:4933427D06Rik 4:intergenic:2810001G20Rik-Gm12290 4:intergenic:Gm23531-Gm26181 4:intergenic:Gm13852-Chchd3 4:intron:Tll2 4:intron:Grin2b 4:intergenic:Gm6686-Chd1 4:intergenic:Slc12a7-Nkd2 4:intergenic:Gm10714-AL593857.1 4:intron:Abca12 4:intron:Ccser1 4:intergenic:Gm12060-0610010F05Rik 4:intron:Adamts18 4:intergenic:Tcerg1l-Mapk1ip1 4:intergenic:Dysf-Cyp26b1 4:intergenic:Trim60-Trim61/Gm10664 4:intergenic:Crybb2-Crybb3 4:intergenic:Gm11658-Rgs9 4:intergenic:4930432M17Rik-F3 4:intron:Mcoln2 4:intergenic:Ccser1-Gm22212 3:intergenic:P2ry10-A630033H20Rik 4:intron:Gabbr2 4:exon:Ccdc107 4:intron:Cenpn 4:intergenic:Eef1e1-Gm22035 4:intron:Rere 4:intergenic:Diap2-Gm14987 4:intergenic:Gm26844-Gm4812 4:intergenic:Pabpc1-Ywhaz 4:intron:Apol10b 4:intron:Apol11a 4:intron:Apol11b 4:intron:Wdr35 4:intergenic:Slc17a4-Snrp1c-ps2 2:intergenic:Mcf2-Atp11c 4:intergenic:Gm6584-Gm20997 3:intron:Camk2d 4:intergenic:Gm25839-4930587A21Rik 4:intron:Pias1 4:intergenic:Pgm2-Gm12702 4:intergenic:Gm26164-Gm10283 4:intron:Gm16838 4:intron:Chd3 4:intergenic:1700017N19Rik-Gm26278 4:intergenic:Prl7b1-Prl7a1 Off-target primers show all... show less...
104 / fw	`TAGTTTGTCTGTACCCTTCT TGG` `.................... ...` Inefficient Enzymes: HpyAV, BslI Cloning / PCR primers	77	87	19	44	66	76	0 - 0 - 0 - 14 - 136 0 - 0 - 0 - 1 - 1 150 off-targets	3:intergenic:1700019E08Rik-Gm13465 4:intergenic:Gm6588-Sez6l 3:intron:Slc26a4 4:intergenic:Mmp15-Gtl3 4:intergenic:Gm26422-Gm22338 4:intergenic:Pcdh9-Gm23324 4:intergenic:Gm9578-Gm22891 3:intergenic:Olfr486-Olfr487 4:intergenic:Duxbl3-4933413J09Rik 4:intergenic:Duxbl2-RP23-100J17.7 4:intergenic:Duxbl1-RP24-222L21.1 4:intron:Epha8 4:intron:Siglec1 4:intergenic:Rps6-ps2-mmu-mir-8110 3:intergenic:Gm15168-Gm5764 4:intergenic:Gm23131-Lphn2 4:intron:Trhr 4:intergenic:Usp13-Pex5l 4:intron:Rtcb 4:intergenic:Ppp1r10/Mir1894-Ppp1r10 4:intergenic:Gm22565-Gm24288 4:intergenic:Trbv21-Trbv23 3:intergenic:Pabpc1-Ywhaz 3:intergenic:Gm23342-Myt1l 4:intron:Stmn2 4:intergenic:Oxgr1-Mbnl2 4:intron:Tbc1d4 4:intron:Tmem117 4:intergenic:Gm22159-Gm25293 4:intergenic:Fndc3c1-Cysltr1 4:intron:Dlgap1 4:exon:H2afz 4:intergenic:Gm23487-Gm1335 4:intergenic:Actrt2-Gm13113 4:intergenic:Pdcd1-Gm23389 4:exon:Cacna1e 4:intergenic:Smlr1-Tmem200a 4:intergenic:Gm8526-Edil3 4:intergenic:Vps37d-Mlxipl 4:exon:Pex11b 4:intergenic:Gm24132-Gm25160 4:intergenic:Gm25987-Gm24834 4:intergenic:Gm15058-Gm6446 4:intron:Cdc45 4:intergenic:Gm2709-Gm16533 4:intron:Itpr1 4:intron:Gramd1a 4:exon:Gm16154 4:intergenic:Traj48-Traj46 4:intergenic:B4galt6-Trappc8 4:intergenic:Gm26861-Gm25496 4:intron:Sema6d 4:intergenic:Cbx8-Cbx4/Gm26508 4:intergenic:Ly6h-Gpihbp1 4:intergenic:4932435O22Rik-Gm11689 4:intergenic:Eif3s6-ps1-Gm11995 4:intergenic:Samd12-Gm26933 4:intergenic:Gm14454-Gm14642 4:intron:Tead1 3:intron:Dlgap2 4:intergenic:Gm10181-Gm10180 4:intron:Nt5dc3 4:intergenic:Stag2-Gm14619 4:exon:Tmem123 4:intergenic:Gm15000-Gm15001 4:intergenic:Glrx3-Gm25798 4:intron:Slc24a4 4:intergenic:Gm6863-Gm22230 4:intron:Acin1 4:intron:Sptbn5 4:intergenic:Pard3-mmu-mir-21c 4:intergenic:Wnt11/Gm8398-Uvrag 3:intergenic:Gm12794-Gm12790 3:intergenic:Gm12800-Gm12794 4:intron:Mid1 4:intergenic:Tex29-A230072I06Rik 4:intergenic:Dph3b-ps-Gm24527 4:intron:Ptchd3 4:exon:Caps2 3:intergenic:Adra2a-Gpam 4:intron:Dnm1l 4:intron:Rbms3 4:intron:Ppfia2 4:intron:Trak1 4:intron:Raf1 4:intergenic:Gm11406-Gm11247 4:intergenic:Gm22825-Zfhx3 4:intergenic:Prr18-Sft2d1/T2 3:intergenic:Mcc-A930012L18Rik/Mcc 4:intergenic:Clec14a-Gm17529 3:intergenic:Gm22211-Gm6181 4:intergenic:Nars2-Gab2 4:intergenic:Gm11922-Map3k7 4:intergenic:Gm24326-Gm25923 4:intergenic:Gm6893-Gm15270 4:intergenic:Pde7b-Ahi1 4:intergenic:Gm4995-Gm15032 4:intron:Gm26691 4:intergenic:Vmn2r59-Hmgb1-ps7 4:intergenic:Gm25714-Gm24997 4:intron:Adarb1 4:intron:Sh3pxd2a 4:intron:1500009C09Rik 4:intergenic:Gm23249-Gm9726/mmu-mir-8099-2 4:intron:Pdcd10 4:intergenic:Gm22547-Gm8919 4:intron:Chn1 4:intergenic:Dnajc1-Gm13354 4:intron:Ptprb 4:intron:Ccdc30 4:intron:Diap3 4:intergenic:Gja1-Gm9795 4:intergenic:Gm11410-Gm11406 4:intron:Fbxw22 4:intron:Fbxw28 4:intron:Fbxw14 4:intron:Mcm3ap 4:intergenic:Gm26889/Svep1-Musk 4:intergenic:Gm24691-Grm8 4:intergenic:Gm15216-Mospd2 4:intron:Fam35a 4:intergenic:AC116451.1-Rpl13a-ps1 3:intergenic:Gm12381-Gm25581 4:intergenic:Gm24614-Stmn2 4:intergenic:Gm11658-Rgs9 4:intergenic:Gm6098-Zbbx 4:intron:Slc27a5 4:intergenic:Gm12008-Gm12009 4:intergenic:Ranbp17-Anp32-ps 4:intergenic:Epha1-Tas2r143 3:intergenic:Rnpc3-Col11a1 4:intergenic:Tmem30b-Prkch 4:intergenic:Gm17540-Gm21915 4:intergenic:Gm2574-Gm24728 4:intergenic:Cd93-Gm14120 4:intergenic:Gm10051-Gm15627 4:intergenic:Gm6098-Zbbx 4:intron:Itsn1 4:intergenic:Gm26184-Dlgap2 4:intergenic:Grik2-Ascc3 4:intergenic:n-R5s46-Ccser2 4:intergenic:Gm12007-Gm12008 4:intergenic:Irx1-Rpl9-ps4 4:intron:Nell1 4:intron:Trpm1 4:intergenic:Gm11993-Abca13 4:intergenic:Sema6a-Gm5506 4:intergenic:Hid1-Cdr2l 4:intergenic:Tbc1d21-4930461G14Rik 4:intron:Dcn Off-target primers show all... show less...
188 / fw	`GTTAGGACTTAGGAACTGAC TGG` `.................... ...` Enzymes: Bse1I Cloning / PCR primers	77	88	51	37	83	88	0 - 0 - 2 - 9 - 94 0 - 0 - 0 - 0 - 4 105 off-targets	4:intron:Sc4mol 2:intergenic:Gm14300-Cdh4 4:intergenic:St6gal1-Gm23430 4:intergenic:Gm26599-Tub 4:intergenic:Gm11820-Gm26250 4:intergenic:Gm10698-Gm25439 4:intergenic:1110038F14Rik-Mb 3:intergenic:Gm11924-4930556G01Rik 4:intergenic:Slc30a1-Rd3 4:intergenic:Trim29-Pvrl1 3:intron:Sptbn1 4:intron:Ano6 4:intergenic:Xpo4-Xpo4/Lats2 4:intergenic:Gm26927-Gm17618 4:intron:Dytn 4:intergenic:Gm24783-Gm16421 4:intron:D230025D16Rik 3:intron:Tmem218 4:exon:Kidins220 4:intergenic:Gm22891-Gm24774 4:intergenic:Gm15337-Nr3c1 4:intergenic:Gm7361-4930584F24Rik 4:intron:Ezh2 4:intron:Zbbx 4:intergenic:Gm25684-Gm9966 4:intergenic:Grxcr1-Gm23605 4:intergenic:Gm26913-Gm4076 4:intergenic:Dclk3-Stac 4:intergenic:Mroh9-Gm22434 4:exon:Ttc25 4:intergenic:Mrps33-Gm26833 4:intron:Sorcs3 4:intron:Pigx 4:intron:Erc2 4:intergenic:Tshz2/A630075F10Rik-Zfp217 3:intron:Oaf 4:intergenic:Gm6634-Gm8604 4:intergenic:Trp63-Gm24928 4:intergenic:Gm7488-Tbl1xr1 4:intron:Aass 4:intron:Gm20543 4:intergenic:Hecw1-Mrpl32 4:intergenic:Brinp1-Gm24697 4:intergenic:Gm8410-Gm6062 4:intergenic:Cyp2c38-Cyp2c39 4:intron:Phka2 4:intergenic:Rpl21-ps5-Gm24508 4:intergenic:4930448K12Rik-Pard3b 4:intergenic:Crabp1-Ireb2 4:intergenic:A930004D18Rik-Gm13330 4:intergenic:Lactb2-Xkr9 3:intergenic:Gm20644-Neurod2 4:intron:Sgcb 4:intron:Kmt2d 4:intron:Akap13 4:intergenic:Mafb-Gm14221 4:intergenic:Pcnxl4-Dhrs7 4:intergenic:Gm12911-Brinp1 4:intergenic:RP23-452E7.2-Irak1bp1 4:intergenic:Gm16685-Gm24614 4:intergenic:Gm22132-Nr5a2 4:intergenic:Gm22914-Skap2 4:intergenic:Gm26077-Olfr16 4:intergenic:Prkrir-Wnt11 4:intron:Ptpn14 4:intergenic:Gm23520-Gm12131 4:intergenic:Gm13660-Gm13655 4:intergenic:Sp2-Sp6 4:intergenic:Gm15866/Gm15865-C1qtnf7 4:intergenic:Sucnr1-Gm24382 4:intergenic:5033403F01Rik-Gm23972 4:intergenic:Snd1-Lrrc4 4:intergenic:Gm7656-Gm24880 4:intergenic:Gm4258-Ptprc 4:intron:Tpm1 2:intergenic:Kcnip1-Kcnmb1 4:intron:Ext1 4:intergenic:Gm9845-n-R5s195 4:intergenic:Park2-Agpat4 4:intron:Vwa8 3:intron:1700034G24Rik 4:intergenic:Nell1-1700081H22Rik 4:intergenic:Gm23452-Gm23900 4:intron:Six1 4:intron:Syngap1 4:intergenic:Tcf7l2-Gm22271 4:intergenic:Ndst1-Rps14 4:intron:Exoc6 4:intergenic:Gm26289-Cycs 4:intergenic:4930505H01Rik-Gm23769 4:intron:Dmxl2 4:intron:Dlgap2 4:intron:Clec2l 4:intergenic:Gorab-Mettl11b 3:intergenic:Pcdh9-Gm23509 4:intergenic:Gm22521-Gm25702 4:intergenic:Gap43-4932412D23Rik 4:intron:Lgr6 4:intergenic:Ism1-Tasp1 3:intergenic:Ajap1-Gm25880 3:intergenic:4933433F19Rik-Dusp26 4:intergenic:Gm12029-Spred2 4:intergenic:Gm9869-Uaca 4:intron:Mrpl3 4:intergenic:Strn3-Ap4s1 Off-target primers show all... show less...
106 / fw	`GTTTGTCTGTACCCTTCTTG GGG` `.................... ...` Inefficient Enzymes: BslI Cloning / PCR primers	74	86	58	46	66	64	0 - 0 - 3 - 21 - 129 0 - 0 - 2 - 2 - 2 153 off-targets	3:intergenic:Gm23591-Speer2 4:intron:Acpl2 4:intron:Akap13 4:intergenic:Arid1b-Tmem242 4:intergenic:Gm25516-9030622O22Rik 4:exon:Lama4 4:intron:Dst 4:intergenic:Gm25072-Micall1 4:intron:Gm14486 4:intergenic:Aqp1-Ghrhr 4:intergenic:Chrm1-Chrm1/Gm22820 2:intergenic:Gm23538-1700110M21Rik 4:intron:Gm15477 3:intergenic:Ankrd1-Pcgf5 4:intergenic:Gm24957-Gm22492 4:intron:Fam19a5 4:intergenic:Tnp1-Pinc 4:intergenic:Gm25369-Akap12 3:intron:Ndst4 3:intergenic:Gm10662-Vmn1r165 3:intergenic:Vmn1r123-Vmn1r124 3:intergenic:Vmn1r132-Gm4175 3:intergenic:Vmn1r160-Gm4214 4:intergenic:4933416E03Rik-9130015L21Rik 4:intergenic:Tcf21-Eya4 4:intergenic:BC028471-Gm20488 3:intergenic:Anxa2-Gm23728 4:exon:Gm14617 4:intergenic:Smco2-Ppfibp1 4:exon:Ldhc 4:intergenic:Pag1-Fabp5 4:intron:Hipk1 4:intron:Hs6st3 4:intron:Tmem108 4:intergenic:Spag9-B230206L02Rik 4:intergenic:RP24-86O15.2-Gm10698 4:intergenic:Zfp329-Zfp110 4:intergenic:Cyp2f2-Gm15883/Cyp2f2 4:intergenic:Tenm2-Gm22127 3:intergenic:Cntn5-Gm25365 4:intron:Fam83g 4:intergenic:Gm13988-Sema6d 4:intergenic:Ccdc33-Gm16130 4:intergenic:Gm17540-Gm21915 4:intergenic:Glrp1-Arl4c 4:intron:Ttc27 4:intron:Kcnh5 3:intergenic:Dck-Mkrn1-ps1 4:intergenic:Mmp15-Gtl3 4:intron:Plxna2 4:intergenic:Gm13332-Armc3 4:intergenic:Sqrdl-Gm13989 4:intron:Plce1 4:intergenic:D930016D06Rik-Gm8258 4:intron:Zfp831 4:intergenic:Atg10-A830009L08Rik 4:intergenic:Adcy5-Gm25179 4:intron:Pex5 4:intron:Tox3 4:intron:Tenm2 4:intergenic:AC113989.1-Gm24780 3:intron:Ccdc93 4:intergenic:Twistnb-Gm23529 4:intron:Nox3 4:intergenic:Nuak1-4930463O16Rik 4:exon:Gm26928 4:intergenic:Olfr343-ps1-Olfr344 4:intergenic:Gm15442-Gm24749 4:intergenic:Tmem144-Fam198b 4:intron:Enoph1 4:intron:Dnajc18 4:intron:Gm12503 4:exon:Prdm11 4:intron:Tnpo1 4:intergenic:Hey2-Gm24168 2:intergenic:Pabpc1-Ywhaz 4:intergenic:Gjb1-Zmym3 4:intergenic:Psmd1-Htr2b 4:intergenic:Gm26976-Cand1 4:intergenic:Gm26976-Cand1 4:intron:Inadl 4:intron:Ank1 4:intergenic:Gm6313-Gm23456 4:intron:Itga11 4:intergenic:Hmgb1-5730422E09Rik 4:intergenic:Magee2-Gm6285 4:intergenic:Ccr7-Smarce1 3:intergenic:Gm14292-Gm14293 3:intron:Frmd4b 4:intergenic:Gm25303-Gm10384 4:intron:B4galt5 3:intergenic:Unc5cl-Gm24071 4:intergenic:Gm23653-Nnmt 4:intron:Csf1 4:intron:0610012H03Rik 4:intergenic:Gm4784-Gm4991 4:intron:Ap1b1 4:intron:Arl8b 4:intron:4930417H01Rik 4:intergenic:Lpar1-Gm12578 4:exon:Sgsm1 4:intron:Ccdc88c 4:intergenic:Tlr9-Alas1 4:intergenic:Mapk8-Ptpn20 4:intergenic:Slc4a1ap-Mrpl33 4:intergenic:Gm22689-Gm9742 3:intergenic:Gm15168-Gm5764 4:intron:Dmd 4:intergenic:Trps1-Eif3h 4:intergenic:Chd9-Gm6658 4:intergenic:Gm13501-Gm13503 4:intron:Etfa 4:intergenic:A730045E13Rik-Gm26714 4:intron:Nfasc 4:intergenic:Ube2nl-Gm25533 4:intergenic:Hmgb1-ps9-Pik3r1 4:intron:Bcmo1 4:intergenic:4930421P07Rik-Zfp518b 4:intergenic:Gm12646-Focad 4:intron:Brd4 2:intergenic:Gm11360-Prl4a1 4:intron:Cmc1 4:intergenic:Zfp853-E130309D02Rik 4:intron:Lphn3 4:exon:Sept4/Gm11492 4:intergenic:Gm16938-B3gnt6 4:intergenic:Gm24602-Cdh11 4:intergenic:Xkr6-Mir598 4:intron:Bcl11b 4:intron:Ifltd1 3:intergenic:Gm23986-Gm24929 4:intron:Retnla 3:intergenic:Gm16599-Cux1 4:intron:Pcdh7 4:intergenic:Gm22689-Gm9742 4:intergenic:Gm11984-Adcy1 4:intergenic:Pdgfra-Gm22084 3:intergenic:Trbv21-Trbv23 4:intron:Vac14 4:intergenic:Sh3tc1-Ablim2 4:exon:Tgfbi 4:intergenic:Ncmap-Gm12990 3:intron:Manbal 4:intron:Grm4 4:intergenic:Qk-Pacrg 4:intron:Tanc1 4:intergenic:n-R5s209-Gm7846 3:intergenic:Nrp2-Ino80d 4:intergenic:Cntn5-Gm25365 4:intergenic:Gm2193-Gm1815 3:intergenic:Gm24326-Gm25923 4:intergenic:Rbm20-Gm16298 4:intergenic:Olfr487-Olfr488 Off-target primers show all... show less...
200 / fw	`GAACTGACTGGTGGCTGTAA GGG` `.................... ...` Cloning / PCR primers	72	82	41	66	60	69	0 - 0 - 0 - 18 - 157 0 - 0 - 0 - 2 - 9 175 off-targets	4:intergenic:Rassf6-Cxcl5 4:intron:Fgf13 4:intron:Ctnna2 4:intergenic:Gm25715-N6amt1 4:intergenic:4732418A04Rik-Scmh1 4:intron:Peli1 4:intergenic:Kcna6/Gm26673-Ndufa9 4:intergenic:Gm6586-Gm10482 4:intergenic:Gm21614-Gm23249 4:intergenic:Tfeb-Mdfi 3:intron:Oas3 4:intron:Ptprt 4:intergenic:Agbl1-Gm23239 4:exon:Marveld2 4:intergenic:Uaca-Gm9869 4:intron:Atp2c2 4:intron:Cd200r3 4:intron:Ipo9 3:intergenic:Irx5-Irx6 4:intron:Sil1 4:intergenic:Gm25460-Gm5263 3:intergenic:Pawr-Gm25591 4:intergenic:mmu-mir-7242-M5C1000I18Rik/C2cd4b 4:exon:Fam189a1 4:intergenic:Epha4-Gm26263 4:intergenic:Ankrd9-Rcor1 4:intergenic:Gm26008-Slc37a3 4:intergenic:Gm23529-Ferd3l 4:intergenic:Fut8-Gm24070 4:intergenic:5S_rRNA-Cops8 4:intergenic:Gm6317-Gm25383 4:intergenic:Tfap2c-Gm22773 4:intergenic:1700017N19Rik-Gm26278 4:intergenic:Ubl3-2210417A02Rik 4:intron:Thrb 4:intron:Baiap2l1 4:intron:Nod2 4:exon:Gm11755 4:intergenic:Slc13a1-Iqub 4:intergenic:Slc22a27-Slc22a28 4:intergenic:Gm25793-Gm8534 4:intergenic:Gm24587-Dcn 4:intergenic:Gm13982-Gm13985 4:intergenic:Spata19-Opcml 4:intergenic:Pced1b-Rpap3 3:intron:Man1c1 4:intergenic:Schip1-Gm10292 4:intergenic:n-R5s42-Fam19a5 4:intergenic:Lamc2-Lamc1 4:intron:Tmod3 4:intergenic:Gm13793-Gm26396 4:intron:Cdk14 4:intergenic:Clca4-Gm6289 4:intergenic:Gm24063-Rps15a 4:intergenic:Serp2-Lacc1 3:intergenic:Gm13552-Tanc1 4:intron:Srrm4 3:intron:Zfpm1 4:intron:Fyn 4:intron:Apba2 4:intergenic:Gm5828-Jph1 3:intergenic:Rbms3-Gm26614 4:intergenic:RP24-375O19.3-Gm23791 4:exon:Dtx1 4:intron:Ebf1 4:intergenic:Cntn5-Gm25365 4:intergenic:Htr1a-Dph3b-ps 4:intergenic:Cnga2-Magea4 4:intergenic:Shank3-Gm15609/Shank3 3:intergenic:Otud7a-Klf13 3:intron:Polr3f 4:intron:Trpm2 4:intergenic:Btg4-Pou2af1 4:intergenic:4933434M16Rik-2610035D17Rik 4:intergenic:Rps19-Cd79a 4:intergenic:Gal-Ppp6r3 4:intron:Elmo1 4:intergenic:Cd109-Gm8116 4:intergenic:Cd36-Gnat3 4:intron:Neu2 4:intron:Thada 4:intron:Utrn 3:intergenic:Tmcc3-Gm16155 4:intron:Fcer2a 4:intron:Pik3c2a 4:intron:Fam189a1 4:intron:Gm684 4:intergenic:Gm26573-Gm13251 4:intergenic:Ube2f-Gm7785 4:intron:Zfp362 4:intergenic:A630031M04Rik-1700112K13Rik 4:intron:Ghrh 4:intron:Dtnb 4:intron:Itgb4 3:intergenic:Nkd2-Trip13 4:intergenic:C1qtnf7-Cc2d2a 4:intergenic:Lpin3-Gm16751 4:intergenic:Mmp20-Mmp7 4:intron:Phf1 4:intron:Wipf3 4:intron:Cdyl 4:intergenic:Pde4b-Gm24869 4:intergenic:Fgf9-Gm25614 4:intron:Etd 3:intergenic:Pdlim2-Sorbs3 4:intergenic:Gm13264-Gm26478 4:intron:Cyp27a1 4:intergenic:Myrip-Eif1b 4:intron:Trpm8 4:intergenic:Iqcf4-Iqcf6 4:intergenic:Mrpl1-Fras1 4:intergenic:RP23-264N13.2-Lrrc1 4:intron:C2cd2 4:intron:Gm13147 4:intron:Gm13154 4:intergenic:Gm26763-Gm13212 4:intron:Frmd6 4:intergenic:Gm12351-Gm12350 4:intron:Phox2b 4:intron:Cacna2d4 3:intergenic:Gm25715-N6amt1 4:intergenic:Gm14645-Gm16436 4:intergenic:Gm12374-Gm12375 4:intergenic:Gm13484-Gm13498 4:intron:Mllt1 4:intron:Syt6 4:intergenic:Dnajc15-Epsti1 3:intergenic:Sall1-Gm6625 4:exon:Wdr66 4:intergenic:Gm8601-Btg1 4:intron:Mrpl23 4:intergenic:Tmed10-Fos 4:intergenic:A930031H19Rik-Gm25261 4:intron:Lama4 4:intergenic:D3Ertd751e-Gm25714 4:intron:Arhgef9 4:intergenic:Gm12011-Vstm2a 4:intron:Tbx5 4:intergenic:Icosl-D10Jhu81e 4:intron:Kat8 4:intergenic:Lgr5-Tspan8 4:intron:Casc1 4:intergenic:Cyp51-Gm9897 4:exon:Ccdc137 4:intergenic:Hspa9-Ctnna1 4:intron:Tsn 4:intron:Pter 4:intron:Gm1972 4:intron:Gm16531 4:intergenic:PVT1_3-Ndufa4 3:intergenic:Man2b2-Ppp2r2c 4:intergenic:Gm26002-Itgb1 4:intron:Gan 4:intergenic:Gm24704-Gm24062 4:intron:Tbxas1 4:exon:Gm498 4:exon:Btbd3 4:exon:Pex2 4:exon:Gm20796 4:intergenic:Cdh9-Gm22032 4:intergenic:Opcml-Gm15606 4:intergenic:Enc1-C430039J16Rik 3:intergenic:Gm12789-Gm12799 4:intergenic:Gm12130-Gm23520 4:intergenic:AC131701.1-Gm17065 3:intron:Ddr2 4:intergenic:Tbc1d21-4930461G14Rik 4:intron:Tdrd12 3:intron:Arhgap22 4:intergenic:D230002A01Rik-Gm24254 4:intron:Dcdc5 4:intron:Asap1 4:intergenic:Gm24848-Txnl1 4:intergenic:C230029F24Rik-PCGEM1 4:intergenic:Gm15082-Atp2b2 Off-target primers show all... show less...
16 / rev	`AGACTATCTCTTCACCAAAA CGG` `.................... ...` Enzymes: BceAI Cloning / PCR primers	71	84	48	43	67	79	0 - 0 - 3 - 18 - 161 0 - 0 - 1 - 2 - 4 182 off-targets	4:intergenic:D3Ertd751e-Gm25714 4:intergenic:Gm23795-Gm5342 4:intergenic:AC161410.1-Haus8 4:intron:Cask 4:intergenic:Gm22895-Gm24713 3:intergenic:D230002A01Rik-Gm24254 4:intergenic:Igkv1-135-Igkv17-134 4:intron:Palld 4:intron:Smoc1 3:intron:Ctage5 4:intergenic:Lrpap1-Adra2c 4:intergenic:Gm23535-n-R5s218 3:intergenic:5033430I15Rik-Gm10113 4:intergenic:Gm15213-Ap1s2 4:intergenic:B230110C06Rik-Oxsm 4:intergenic:Gm13498-Rnd3 4:intergenic:Sept9-Gm11729 3:intergenic:Mdfic-Tfec 4:intergenic:Gm26354-Gm7536 4:intergenic:D130020L05Rik-Kif4-ps 4:intergenic:Ttll7-Gm23131 4:intergenic:Gm23900-Zfp804a 4:intron:Fancl 4:intergenic:Hmgcr-5S_rRNA 4:intron:Zfpm2 3:intergenic:Gm22507-Trhde 4:intron:Lrrk2 4:exon:Pth1r 4:intergenic:Tmem108-Nphp3 4:intergenic:Gm8410-Gm6062 3:intergenic:Gm22782-Rpl10l 4:intron:Nedd4l 4:intron:Ccdc92 4:intron:Sos2 4:intron:Ddx42 4:intron:1700012P22Rik 3:intron:Thsd7b 4:intergenic:Gm17131-Tmem132d 4:intron:Dmd 4:intergenic:Gm4409/Lrrtm4-Lrrtm4 4:intergenic:Ftx/FTX_1-Gm9159 4:intron:Auh 4:intergenic:Gm22492-Pdcd6ip 4:intron:Gucy1a3 4:intergenic:Gm12760-Gm12779 3:intergenic:Sucla2-Gm21750 4:intergenic:Gm13692-Gm13702 4:intergenic:Gm12638-Gm12637 4:intergenic:Gm24350-Gm13464 4:intergenic:Gm25550-Cdh9 4:intergenic:Cyp7b1-Gm23686 4:intergenic:Akap17b-Gm6274 4:exon:Dgke 4:intergenic:Gm25974-Epha4 4:intergenic:Irf2bp2-Tomm20 4:intron:Mob4 4:intergenic:Gm6984-Htr2a 4:intergenic:Gpr82-Cask 4:intergenic:Gm26026-Gm5513 4:intron:Rps6kc1 4:intron:Faf1 3:intergenic:Cyp2c66-Cyp2c53-ps 4:intergenic:Il9-Fbxl21 4:intergenic:Scgb1b27-Gm12760 4:intergenic:Fam120c-Gm15148 4:intergenic:Gm26632-Rbpms 4:intergenic:Adtrp-Gm22157 4:intergenic:Gm24280-Rbm26/Gm17066 4:intergenic:Gm13499-1700057H21Rik 4:intergenic:Gm14567-Gm16423 4:intergenic:Mcc-A930012L18Rik/Mcc 3:intron:Nrxn3 4:intron:Pbrm1 4:intergenic:Gm22692-Gm11461 4:intron:Dennd1a 4:intergenic:Nox3-Gm23186 4:exon:Kcnk15 3:intergenic:Gm11542-Gm11541 4:intergenic:Gm11923-Gm11925 4:intergenic:Khdrbs1-Gm12967 4:intron:Atp8b4 4:intergenic:Gm24063-Rps15a 4:intergenic:Gm22016-Cdc73 4:intron:Gm13377 4:intron:Pde10a 4:intron:Depdc5 4:intron:4930412C18Rik 4:intergenic:Gm26190-B3gat1 4:intergenic:Adamts8-Zbtb44 3:intron:Kctd16 4:intergenic:Gimap3-Tmem176b 3:intergenic:Gm23447-Gm23773 4:intergenic:Qk-Pacrg 4:intergenic:Efnb1-Gm14812 4:intergenic:Rap1b-Mdm1 4:intron:B4galt1 4:intron:Tnfrsf19 4:intergenic:Fau-ps1-Macrod2 4:intron:Ldlrad4 4:intergenic:Gm22593-Mettl4 4:intergenic:Lrpap1-Adra2c 4:intron:Pole2 4:intron:Gm15506 4:intron:Enpp1 4:intergenic:4930428N03Rik-Gm10398 4:intron:Rundc3b 4:intergenic:Cbfa2t2-ps1-Gm23331 4:intron:BC018507 3:intron:Rabgap1l 4:intergenic:Sema6d-4930517E11Rik 4:intergenic:A630073D07Rik-Tas2r120 4:intron:Runx1 4:intergenic:Gm6984-Htr2a 4:intergenic:Auh-n-R5s54 4:intergenic:Eif3s6-ps1-Gm11995 2:intergenic:Mmrn1-A730020E08Rik 4:intergenic:Gm23735-Fndc3a 4:intergenic:Focad-Gm12645 4:intron:Mfi2 4:intergenic:Gm25702-Gm5473 4:intergenic:Gm11820-Gm26250 4:intergenic:Tmem27-n-R5s18 4:intergenic:Gm13005-Gm13002 4:intergenic:Iqcf4-Iqcf6 4:intron:Slc4a10 4:intergenic:Gm14032-Gm14033 4:intergenic:Sox4/Gm26735-A330102I10Rik 4:intron:Afap1l2 4:intron:Fbxw5 4:intron:Enox1 4:intron:Pvrl1 4:intergenic:Gm7589-Adpgk 4:intergenic:Slc5a3-Gm16310 3:intergenic:Gm14264-Dok5 2:intergenic:Poteg-Gm1698 4:intron:Dab1 4:intergenic:Gm11042-Kcnh5 4:intergenic:Gm24350-Gm13464 4:intergenic:Stim2-Gm24235 4:intergenic:Dscaml1-Gm22069 3:intron:Cep85l 4:intergenic:Gm22227-Zfp703 4:intergenic:Gm23848-Trps1 4:intergenic:Rasgef1c-Rnf130 4:intron:Fstl4 4:intergenic:Hpgd-Gm24375 4:intron:Kidins220 4:intergenic:RP24-86O15.2-Gm10698 4:intergenic:Gm23038-4930570G19Rik 4:intergenic:AC116451.1-Rpl13a-ps1 4:intergenic:Gm25850-Gm23646 4:intergenic:Gm25935-Gm22593 4:intron:Slc34a2 3:intergenic:Bmp15-Shroom4 4:intergenic:Rhob-Slc7a15 4:intergenic:Gm16541/R3hcc1l-R3hcc1l 2:intron:Nim1 3:intergenic:Gm15018-Nxf2 4:intron:Grm1 4:intergenic:2010001M06Rik-Stx1a 4:intron:Hipk2 4:intergenic:Ppp4r2-Pdzrn3 4:intron:Ppa2 4:intergenic:RP24-325L16.1-RP23-417D20.1 4:intron:Rspo3 4:intron:Fer1l6 4:intergenic:Adcy2-Gm26844 4:intron:Sim1 4:intron:Tmem127 4:intergenic:Rpl31-ps23-Ppp1r3b 4:intergenic:Gm11867-Gm25971 4:intergenic:Gm23987-Fam135b 4:intergenic:Uchl5-Rgs2 4:intergenic:Slc13a1-Iqub 4:intergenic:Atxn7l1-Gm11052 4:intron:Gm101 4:intergenic:Gm12506-Gm12519 4:intron:Gm12064 4:intergenic:Gm25614-Gm9022 4:intergenic:3100003L05Rik-Gm21957 4:intergenic:Gm24319-Psat1 4:intergenic:Fign-Gm13575 Off-target primers show all... show less...
191 / fw	`AGGACTTAGGAACTGACTGG TGG` `.................... ...` Enzymes: Bse1I Cloning / PCR primers	71	86	71	64	67	81	0 - 0 - 5 - 20 - 177 0 - 0 - 2 - 2 - 5 202 off-targets	4:intron:Ttc8 4:intergenic:Gm25325-Gm15986 4:intergenic:Gm13200-Ubiad1 4:intergenic:Abcd2-CN725425 4:intron:Dmd 3:intergenic:Cggbp1-Gm9061 2:intergenic:Gm14300-Cdh4 4:intron:Cd82 4:intergenic:Trim41-Gm12184/Trim7 4:intron:Camta1 4:intron:Plb1 4:exon:Fau-ps2 3:intergenic:Crry-ps-AC161214.1 4:exon:Zfp282 4:intergenic:Tacr3-Gm24048 4:intron:Slc1a2 3:intergenic:Mir3963-Eltd1 4:intergenic:Ankrd12-Ndufv2 4:intron:Hs6st3 4:intron:Sfxn5 4:intergenic:Apoo-ps-Zswim6 4:intergenic:1700017N19Rik-Gm26278 4:intron:Synpo2 4:intergenic:Gm23181-Fam174a 3:intergenic:Gm8421-Gm23945 4:intergenic:Tcf21-Eya4 4:intergenic:Chst10-Nms 4:intergenic:Gm8730-Cdh5 3:intron:Ppp3ca 3:intergenic:Galnt13-Gm14032 4:intergenic:Gm15005-Gm15004 4:intergenic:Olfr718-ps1-Rbak 4:intergenic:Gm24063-Rps15a 4:intergenic:Atxn7l1-Gm11052 4:intergenic:Gm14344/Gm14339-Gm14342 4:intergenic:Gm13323-H2afb1 4:intron:Ddah1 4:intron:Zfp316 4:intron:Atg3 4:intron:Ptprh 4:intron:Ntrk3 3:intergenic:Pcsk1-Gm4149/Mir682 4:intron:Gns 4:intergenic:Otud7a-Klf13 4:intergenic:Gm22120-Vrk1 4:intergenic:Gm25403-March1 4:exon:1700112J16Rik 4:exon:Bcorl1 4:intergenic:Gm15202-Gm26317 3:intron:Sgcb 3:intergenic:Otos-Gpc1 4:intergenic:Gm22507-Trhde 4:intron:Mcph1 4:intron:Lonrf2 4:intron:Atp8b4 4:intron:Ctnna3 4:intergenic:Gm14619-Sh2d1a 4:intron:Scn2a1 2:intergenic:Crabp1-Ireb2 4:exon:Entpd8 4:intron:Elavl3 4:intergenic:Gm13017-Gm13021 4:intron:C2cd2 4:intergenic:Camsap2-9230116N13Rik 4:intron:Lrp1b 4:intergenic:Gm13066-Clstn1 4:intron:Macrod2 4:intron:Cyp26b1 4:intergenic:Gm22642-Efna3/Gm15998 4:intergenic:Gm26181-Triml1 4:intergenic:Agap1-Gm25180 3:intergenic:mmu-mir-6343-Gm25974 4:intergenic:Mrps33-Gm26833 4:intron:Mgmt 4:intron:Mapk10 3:intergenic:Zfp108-Zfp93 4:intron:Ralgapa2 4:intron:Gabra4 4:intergenic:Tshz3-Zfp536 4:intergenic:Rps10-ps2-Gm9697 4:intergenic:Gm9697-2310042E22Rik 4:intron:Cd163 4:intron:Suz12 4:intron:Dock4 4:intron:4930552P12Rik 4:intron:Grin2a 4:intergenic:Mir3971-Slc43a2 3:intron:Camta1 4:intron:Itgae 4:intron:1700026J12Rik 4:intron:Tbc1d22a 4:intergenic:Cdcp2-Gm12802 4:intergenic:Tspyl5-Mtdh 4:intergenic:Mterfd1/Ptdss1-Ptdss1 4:intergenic:Gm14085-Gatm 2:intergenic:Gm6317-Gm25383 4:intergenic:Gm26373-Kcnd2 4:intergenic:Rab33b-Setd7 4:intergenic:Olfr902-Olfr904 4:intergenic:Tm4sf20-Gm22396/Agfg1 4:intergenic:Mapk13-Brpf3 4:intron:Fbxl17 4:intron:Dennd3 4:intergenic:Gm15943/Fbxo32-Klhl38 4:intergenic:Gm24345-Gm22401 4:intron:Sorcs3 4:intergenic:Cmpk2-Gm9866 4:intergenic:Gm15668-Kif22-ps 4:intergenic:Pprc1-Nolc1 4:intron:Rab8a 3:intron:Trim2 4:intergenic:1110035M17Rik-1110035M17Rik/Zfp652 4:intergenic:Gm17473-Rspo2 4:intergenic:Gm14105-Gm14077 4:intergenic:Ctdp1-Nfatc1 4:intron:Sh3pxd2a 4:intergenic:Otos-Gpc1 4:intergenic:Gm6277/Cables1/Mir1901-Cables1 4:intergenic:Dok6-Gm27037 4:intron:Atxn1 3:intergenic:Gm13470-Gm25264 4:intergenic:4930467D21Rik-Antxr2 4:intergenic:4930474H06Rik-Gm22959 4:intron:Ndufs4 4:intergenic:Gm24633-Gm25568 4:intergenic:Gm22181-Gm26994 2:intron:Ubash3b 4:intron:2210015D19Rik 4:intron:Rai14 4:intergenic:5730522E02Rik-Gm22868 4:intergenic:Cndp2-D030046N08Rik/Fam69c 4:intron:Akap2 4:intergenic:Pvt1-Gm24810 4:intergenic:Gm13747-Olfr1162 3:intergenic:4933433F19Rik-Dusp26 4:intron:Kifap3 4:intergenic:Cnot10-Dync1li1 4:intron:Miat 4:intergenic:Gm17399-Gm22198 4:intergenic:Edem1-Gm20387 4:intergenic:Fam227b-Gm26496 4:intergenic:Gm22583-Ep400 4:intron:Zfp592 3:intron:Pik3ca 4:intron:BC005624 4:intron:Syngap1 4:intron:Sh3bp5 4:intergenic:Gm10710-Gm22342 3:intergenic:4930448K12Rik-Pard3b 3:intergenic:Tbc1d5-Satb1 4:intergenic:A330050F15Rik-Gm22967 4:intergenic:Igsf21-Gm9867 4:intron:Ddx31 4:intergenic:Neurog1-Cxcl14 4:intergenic:Gm5909-Gypa 4:intron:Fads2 4:intergenic:Gm26181-Triml1 2:intron:Tpm1 3:intergenic:Prkag2-Gm15552 3:intergenic:Gm14263-Gm14266 4:intergenic:Gm25574-Ankrd50 4:intergenic:Gm12896-Gm12897 4:intergenic:Tex14-ACEA_U3 4:intergenic:Tuba3a-Ltbr 4:intron:Sh2d1b1 4:intergenic:Ttll7-Gm23131 4:intergenic:Gm12895-Gm12877 4:intron:Cacna1a 4:intron:Plekha6 4:exon:Atrn 4:intron:Adcy7 4:intergenic:Gm15754-Med13l 4:intron:2810442I21Rik 4:intron:Ikzf1 4:intergenic:Gm11351-Gm11352 4:intergenic:Glrx3-Gm25798 4:intergenic:Fstl5-Gm23644 4:intergenic:Mfsd1-Iqcj 4:intergenic:RP24-308I2.1-RP24-86O15.2 4:intergenic:Map2-Unc80 4:intergenic:Gm8116-Col12a1 4:intron:Myh15 4:intergenic:Gm5117-Nrg1 4:intergenic:Gm14271-Gm14274 4:intergenic:Cpsf4l-Cdc42ep4 4:intergenic:Gm12878-Gm12892 4:intergenic:Aff3-Lonrf2 4:intron:9530034E10Rik 4:intergenic:Ppp3ca-Gm26107 4:intron:Panx2 4:exon:Pml 4:intergenic:Gm23342-Myt1l 4:intergenic:Grm1-Shprh 4:exon:Iqsec1 4:intron:Kmo 4:intron:Shisa9 4:intergenic:Gm13321-Pter 4:intergenic:Mettl21e-Gulp1 4:intergenic:Gm23189-Gm15671 4:intergenic:Gm23429-Gm24304 4:intergenic:Sos1-Cdkl4 4:intron:Traf3ip1 Off-target primers show all... show less...
105 / fw	`AGTTTGTCTGTACCCTTCTT GGG` `.................... ...` Inefficient Enzymes: HpyAV, BslI Cloning / PCR primers	70	86	27	41	64	46	0 - 0 - 3 - 17 - 183 0 - 0 - 1 - 3 - 1 203 off-targets	4:intergenic:Gm25516-9030622O22Rik 4:intergenic:Gm4409/Lrrtm4-Lrrtm4 4:intergenic:Hrh4-Gm22251 4:intron:Spock1 4:intron:Fem1c 4:intergenic:Gm16599-Cux1 4:intergenic:Ptbp2-Gm24744 3:intron:Zfp831 4:intergenic:Gm14050-Gm14058 4:intergenic:Adm-B430319F04Rik/Ampd3 4:intergenic:Gm17540-Gm21915 4:intron:Acin1 4:intergenic:Trbv21-Trbv23 4:intergenic:Gm23056-Thsd7b 4:intergenic:Nrp2-Ino80d 4:intergenic:Csprs-AC132444.6 4:intron:4931429P17Rik 4:exon:Ppp1r10 4:intron:Slc10a7 4:intergenic:Gm9341-Gm24444 4:intergenic:Ppp2r3a-Gm24638 4:intergenic:Ankrd1-Pcgf5 3:intergenic:PCGEM1-Gm25843 4:intergenic:Amotl1-Piwil4 4:intergenic:Gm94-Spink5 4:intergenic:Il1r2-Il1r1 4:exon:Pip5k1c 4:intergenic:Zeb2-VIS1 4:intron:Atxn1 4:intergenic:Gm25824-Gm26977 4:intron:Park2 4:intron:Mfsd7b 4:intergenic:Dnah11-Sp4 4:intergenic:Gm26463-Gap 4:intron:Zfhx4 4:exon:Frmpd4 4:intergenic:Rgs1-Rgs21 4:intron:Ptprt 4:intergenic:Slc39a10-Gm4852 4:intergenic:Gm23900-Zfp804a 4:exon:H2afz 4:intergenic:Gm25609-Gm23534 4:intron:Exoc6b 4:intergenic:Gm14450-Gm26618 4:intron:Epha6 4:intergenic:Rock1-Greb1l 3:intron:Slc24a4 3:exon:Jmjd7/Pla2g4b 4:intergenic:Rnpc3-Col11a1 4:intergenic:Gm16542-Fhit 4:intron:Diap3 4:intron:Ccdc50 3:intron:BC051142 3:intron:Dlgap2 4:intergenic:Tex29-A230072I06Rik 4:intergenic:Usp13-Pex5l 4:intergenic:BC048679-2900076A07Rik 4:intergenic:Sec16b-Brinp2 3:intron:Grin3a 4:intergenic:B3gnt6-Acer3 4:intergenic:Gm9966-Tenm4 4:intron:Slc26a4 4:intergenic:Eif4g2-Galnt18 4:exon:Ckap2 4:intergenic:Efnb1-Gm14812 4:intergenic:Gm4995-Gm15032 4:intron:Myof 4:exon:Nampt 4:intron:Bicc1 4:intron:Gm16039 3:intergenic:Ube2nl-Gm25533 4:intron:Mocos 4:intergenic:Gm13326-Gm13325 4:intron:Lin28b 4:intergenic:Gm22565-Gm24288 4:intergenic:Smad3-Smad6 4:intergenic:Gm26861-Gm25496 4:intergenic:Cacna2d3-Selk 4:intergenic:Gm25715-N6amt1 4:intergenic:Tmem30b-Prkch 4:intergenic:4931417E11Rik-Gm26179 4:intergenic:Mmp15-Gtl3 4:intergenic:Wnt11/Gm8398-Uvrag 4:intergenic:Gm6410-Shcbp1 4:intergenic:Rnh1-Gm22019 3:intron:Lepre1 4:intron:Gas6 4:intron:Abca4 4:intergenic:Bmper-Npsr1 4:intergenic:Chd9-Gm6658 4:intron:Gpc6 4:intron:Bcmo1 4:intron:Tenm1 4:intergenic:4931417E11Rik-Gm26179 4:intron:Enpp1 4:intron:Mcm3ap 4:exon:Lama4 4:intron:Pnrc1 4:intergenic:Agbl1-Gm23239 4:intergenic:Vldlr-Kcnv2 4:intergenic:Gm26123-March1 3:intergenic:Gm22159-Gm25293 4:intergenic:Rpl35-ps1-Scn9a/Gm13629 4:intergenic:4930554G24Rik-Gm12006 4:intergenic:Gm25602-Tbc1d32 3:intergenic:Slc16a7-Lrig3 4:intergenic:1700116B05Rik-Gm24925 4:intergenic:Gm11846-n-R5s182 4:intron:Bcl11b 4:intergenic:Gm25609-Gm23534 4:intergenic:Gm22917-Gm26245 3:intron:Arhgef38 4:intron:Gfod1 3:intergenic:Gm2709-Gm16533 4:intron:Stxbp5 2:intergenic:Alcam-Gm25723 4:intron:Ccdc141 4:intergenic:Cep89-Slc7a9 4:intergenic:Pot1a-Gm24691 4:intergenic:Iglv1-Iglc2 4:intron:Mtus2 4:intron:Smurf2 4:intergenic:Gm5449-Drd1a 4:intergenic:Ncam2-Gm23355 4:intergenic:Unc5cl-Gm24071 2:intergenic:Pabpc1-Ywhaz 4:intron:Gpat2 4:intergenic:Ccser1-Gm22212 4:intron:Atg16l1 4:intron:Lrriq1 4:intron:Fbxw14 4:intron:Fbxw22 4:intron:Fbxw28 4:intron:Dcn 2:intergenic:Gm24734-Nog 4:intron:Cmbl 4:intergenic:Gnat3-Gnai1 4:intergenic:Gm22903-Gclm 4:intron:Usp34 4:intron:Dysf 4:intron:Atp6v0d1 4:intergenic:Gna13-9930022D16Rik 3:intron:D630045J12Rik 4:intergenic:Gm14738-Rpl10-ps4 4:intergenic:Rpl31-ps25-AC135509.1 3:intron:Slc9a9 4:intron:Dnah11 4:intergenic:RP23-175E16.1-RP24-225I21.1/Kirrel3 4:intron:Btbd11 4:intron:Fdft1 4:intergenic:Fgf9-Gm25614 4:intron:Ccdc141 4:intergenic:Grik2-Ascc3 4:intron:Mlst8 4:intergenic:9530036O11Rik-Rnf32 4:intergenic:Gm16329-Gm16327 4:intergenic:Gm24326-Gm25923 4:intron:Slc9a3r1 4:intergenic:Gm12610-Dmrta1 4:intron:Oxnad1 4:intron:Dnm1l 4:intergenic:Gm26937-Lipi 4:intergenic:Tmem45b-Barx2 4:intergenic:Gm23234-Shq1 4:intergenic:Lrrc8c-Lrrc8d 4:intergenic:Gm11993-Abca13 4:intron:Retnla 4:intron:Map4k4 3:intergenic:4931417E11Rik-Gm26179 4:intergenic:Gm14688-Gm4910 4:intergenic:Gm6427-Gm6429 4:intergenic:Rad51b-Zfp36l1/Gm26669 4:intron:Adarb1 4:exon:Chka 4:intron:Anxa3 4:intron:Mthfd1 4:intron:Mtfr2 4:intergenic:Gm14174-Gm14172 4:intergenic:Tcf21-Eya4 4:intron:Zfyve28 4:intergenic:Gm5839-Gm25440 4:intron:Nrde2 4:intron:Dgki 3:intergenic:Gm15168-Gm5764 4:intergenic:Gm22227-Zfp703 4:intergenic:n-R5s46-Ccser2 4:intron:Gfod1 4:intron:Mgat5 4:intergenic:Calcoco1-Hoxc13 4:exon:Slc12a8 4:intergenic:Gm12800-Gm12794 4:intergenic:Gm12794-Gm12790 4:intergenic:Serpinh1-Gdpd5 4:intergenic:Sox2ot-mmu-mir-6378 4:intron:Fam20c 4:intron:Cars 4:intergenic:Gm22908-Gm4425 4:intergenic:3110053B16Rik-Gm21989 4:exon:Gm9286 4:exon:Gm6838 4:intergenic:Ptafr-Eya3 4:intergenic:Ctdp1-Nfatc1 4:exon:Gm20472 Off-target primers show all... show less...
199 / fw	`GGAACTGACTGGTGGCTGTA AGG` `.................... ...` Cloning / PCR primers	69	85	41	40	60	83	0 - 0 - 4 - 22 - 120 0 - 0 - 1 - 1 - 4 146 off-targets	4:exon:Grin2b/Gm14329 2:intergenic:Irx5-Irx6 4:intron:Frmd6 4:intergenic:Dapk2-Herc1 4:intron:Parg 2:exon:Dgkz 4:intergenic:Gm21232-Gm27044 3:intergenic:Gm13552-Tanc1 3:exon:Dtx1 4:intergenic:Gprc5b-Gpr139 4:intron:2610035D17Rik 4:intron:Zfp42 4:intergenic:Mir148a-Gm26677 4:intergenic:Myrip-Eif1b 4:intergenic:Fbxo15-Timm21 4:intron:Gm6498 3:exon:Gm11755 3:intron:Ddr2 4:intergenic:Gm15399-Gm9372 3:intergenic:Gm16314-Gnb1l 3:intron:Slc2a13 4:intergenic:Crh-4632415L05Rik 4:intron:Tex9 4:intron:Gm17399 4:intron:Ubxn11 3:intergenic:Gm15397-Gm6407 4:intergenic:Tmcc3-Gm16155 3:intergenic:Gm5828-Jph1 4:intron:B4galnt3 4:intergenic:Socs6-Rttn/mmu-mir-6359 4:intergenic:Ccnl1-Veph1 4:intron:Slc12a5 4:intergenic:Hlcs-Gm23062 4:intergenic:Gm26585-Slc2a12 4:intergenic:Tbc1d2b-Adamts7/Tbc1d2b 4:intergenic:1700063H04Rik-Rimklb 4:intergenic:Gm22673-Gm23059 4:intergenic:Pdcd1-Gm23389 4:intron:Pafah2 4:intron:Pxdn 4:intergenic:Tox-Gm11804 3:intergenic:Gm13982-Gm13985 3:intergenic:Gpr144-ps-Nr5a1 4:intergenic:Gm9630-Gm24080 4:intron:Pax7 4:intron:Prmt5 4:intron:Pp2d1 4:intron:Arhgap22 4:intergenic:2310081O03Rik-Gm12518 3:intergenic:Pawr-Gm25591 4:intergenic:Gm11900-Fhl5 4:intron:Mtmr12 4:intergenic:Gm26002-Itgb1 4:intergenic:Gm15723-Gm25319 4:intron:Cenpw 4:intron:Rims1 4:intergenic:Cd109-Gm8116 4:intergenic:Gm10231-Gm26595 4:intron:Jph3 4:intron:Uhrf1 4:exon:Tnfrsf1b 4:intron:Arl15 4:intergenic:Gm26053-Dlg1 3:intron:Mgmt 2:intergenic:Gm6644-Plcl1 4:intron:Man1c1 3:intron:Arhgap10 4:intergenic:Pdlim2-Sorbs3 4:intergenic:Gal-Ppp6r3 4:intron:Ntn4 4:intron:Epsti1 4:intergenic:Rassf10-Gm23662 4:intron:Fam129b 4:intergenic:Gm12413-Sh3gl2 4:intergenic:Timm8a2-A330035P11Rik 4:intron:Arhgef4 4:intron:Gm684 4:intergenic:Kcna6/Gm26673-Ndufa9 4:intergenic:Gm23752-Gm24815 4:intron:Zfp106 3:intergenic:D3Ertd751e-Gm25714 3:intron:Zfpm1 2:exon:Fndc4 4:intergenic:Gm9992-Gm25872 4:intergenic:Gm22508-Ccdc68 4:intergenic:Cdcp2-Gm12802 4:intergenic:Gpr65-Kcnk10 4:intergenic:Cnpy1-Rbm33 4:intergenic:Gm23821-Tgfb2 4:intron:Taok3 4:intergenic:Rbms3-Gm26614 4:intergenic:Iqcf4-Iqcf6 4:intergenic:Gm15011-Rbm41 4:intergenic:Gm26573-Gm13251 4:intergenic:Hnf4a-Ttpal 4:intergenic:Gm3272-Mmp2 4:intergenic:Gpr142-Gprc5c 4:exon:Fam192a 4:intergenic:Gm15831-Spink2 4:intergenic:Gm8330-Hivep2 4:intergenic:Gm8601-Btg1 4:intergenic:Mrpl1-Fras1 4:intergenic:Gm12043-Gm12044 4:intron:Tsn 4:intron:Arhgef10l 3:intergenic:Nudt10-Bmp15 3:intergenic:Gm26618-Nudt11 4:intergenic:Insig1-En2 4:intergenic:Gm16722-Mecom 4:intergenic:Gm11241-Ptprd 4:exon:Idh3a 4:intergenic:Gm12789-Gm12799 4:intron:Acta2 4:intergenic:Gm8177-Tm4sf1 4:intergenic:Tspan15-Tacr2 4:intergenic:Gm13529-9430024E24Rik 3:intergenic:Gm8775-Gm23976 4:intergenic:Gm26763-Gm13212 4:intron:Gm13147 4:intron:Gm13154 4:intergenic:Gm22911-Rfpl4b 3:exon:Slc25a42 4:intron:Arhgap24 4:exon:Gm498 4:intergenic:Gm25845-Celf4 4:intergenic:Rpe65-Wls 3:intron:Itgb4 4:intergenic:1110051M20Rik-Gm13787 4:intergenic:Ttc39b-Gm11413 3:intron:Prickle2 4:intron:Ryr2 4:intron:Cd300a 4:intergenic:Man2b2-Ppp2r2c 4:intergenic:Pde4b-Gm24869 4:intergenic:Gm26295-Gm25375 4:intergenic:Gm10220-Gm7361 4:intron:Tanc2 4:intergenic:Nrbf2-Egr2 4:intergenic:1700057G04Rik-Plscr4 4:intergenic:Mepe-Spp1 4:intergenic:Ect2-Gm25152 4:exon:Gigyf1 4:intergenic:Gm12878-Gm12892 4:intergenic:Gm12893-Gm12895 3:intergenic:Rln3-Gm10643 4:intergenic:C1qtnf2-Ccnjl Off-target primers show all... show less...
130 / rev	`TGAAAAGTGTTAAGACTTGA AGG` `.................... ...` Enzymes: DseDI, AgsI Cloning / PCR primers	65	72	42	16	73	87	0 - 0 - 7 - 28 - 208 0 - 0 - 2 - 0 - 3 243 off-targets	4:intergenic:Gm13914-Rpl35a-ps4 4:intergenic:Spata19-Opcml 4:intron:Grk5 3:intergenic:Gm17361-Gm21616 3:intergenic:Rps12-ps18-Astx1b 3:intergenic:Rps12-ps17-Astx4c 3:intergenic:Rps12-ps6-Astx1a 4:intergenic:D19Bwg1357e-Gm24782 4:intron:Rims1 4:intergenic:Tsg101-Uevld 4:intron:Gatad2b 3:intron:Ehbp1 4:intron:Adamts6 2:intergenic:Fstl1-n-R5s34 4:intergenic:Anxa2-Gm23728 4:intergenic:Gm10797-Zfp365 4:intergenic:Gm25480-Gm11220 4:intergenic:Gm12015-Gm23959 4:intron:Rnf185 4:intron:Alcam 4:intergenic:Gm24680-4921530L21Rik 4:intergenic:Gm22718-Gm24213 4:intron:Tanc2 4:intron:Trank1 4:intron:Slc15a3 4:intergenic:Gm15400-Gm15399 4:intergenic:Has2os-Gm26178 4:intergenic:Wnt5a-Cacna2d3 4:intron:Pan3 3:intron:Agk 2:intergenic:Nap1l3-Gm14947 4:intergenic:Bhlhb9-Gm15016 4:intron:Tmem232 4:intergenic:Gm16080-Ptpru 4:intron:Cadm1 4:intergenic:AC175117.1-Hnf4g 4:intergenic:Gm17467-Rps12-ps23 4:intergenic:Rps12-ps11-Gm17584 4:intergenic:Astx5-Rps12-ps20 4:intergenic:Rps12-ps13-Gm17693 4:intergenic:Gm17412-Rps12-ps22 4:intergenic:Rps12-ps14-Gm17522 4:intergenic:Gm14951-Rps12-ps21 4:intergenic:Rps12-ps16-Gm17267 4:intergenic:Rps12-ps15-Gm17361 4:intergenic:Gm17361-Gm21616 4:intergenic:Rps12-ps12-Gm17469 4:intergenic:Immp2l-Gm25248 4:intron:Adamtsl3 3:intron:H2-Eb2 4:intron:Rgs7 4:intergenic:E330017A01Rik-Cpox 4:intergenic:Gm13466-Gm23072 4:intergenic:4932415D10Rik-Gm4924 4:intron:Mkx 4:intergenic:Etv1-Gm24069 4:intron:Ncoa1 4:intergenic:Rpl30-ps8-Foxn3 4:intergenic:Gm7108-4930415L06Rik 4:intron:Cacna2d3 4:intergenic:Gm23708-G6pd2 4:intron:2310009B15Rik 4:intergenic:Tnni3k-Fpgt 4:intergenic:Gm10736-Gm15286 4:intron:Alms1 4:intergenic:Gm25460-Gm5263 2:intron:Nebl 4:intergenic:Stk35-Tgm3 2:intergenic:Masp1-Rtp4 4:intergenic:Gm20581-Gm20579 2:intron:Zscan21 3:intergenic:Gm24326-Gm25923 4:intergenic:Serpina10-Serpina6 4:intron:Arl15 4:intron:Dock11 4:intron:Ppp3ca 4:intergenic:Gm2824-9230109A22Rik 4:intron:Ube3c 4:intergenic:Ostm1-Gm15199 4:intergenic:Ugt3a2-Capsl 4:exon:Cdr2 4:intergenic:RP24-421H18.1-RP24-421H18.1/RP24-421H18.2 4:intron:Erc2 4:intergenic:4631405K08Rik-Plxna2 4:intron:Secisbp2l 4:intergenic:Gm13988-Sema6d 4:intergenic:CT030233.1-Gm20675 4:intron:Ptpn12 3:intergenic:Cradd-Socs2 4:intergenic:Amigo2/Pced1b-Pced1b 3:intron:Cr1l 4:intergenic:Mbd2-Dcc 4:intergenic:Sorcs2-mmu-mir-7689 4:intergenic:Trhde-4930473D10Rik 4:intergenic:mmu-mir-6411-Gm22945 4:intergenic:Nell1-Ano5 4:intergenic:Gm24774-Pcdh17 4:intergenic:Sox2ot-Gm5708 4:intron:Sema6a 4:intron:Hs3st5 4:intergenic:Gm5152-Gm17091 4:intergenic:Mcph1-Agpat5 4:intron:Cops2 4:intergenic:Olfr299-Olfr298 4:intergenic:Trav6-1-Trav6-2 4:intergenic:Fyn-Gm16365 4:intergenic:Gm12450-Spag17 4:intron:Ulk4 4:intergenic:Vmn1r-ps14-Vmn1r-ps15 4:intergenic:n-R5s176-Rbm19 4:intergenic:Gm12680-Sh3gl2 4:intergenic:Pcdhb20-Pcdhb21 4:intergenic:Gm12415-Cntln 4:intergenic:Tenm3-Gm25830 4:intergenic:Gm12381-Gm25581 4:intron:Bmpr1a 4:intron:Sgol1 3:intergenic:Gm11846-n-R5s182 3:intergenic:Gm24987-Socs6 4:intergenic:Gm826-Mafb 4:intergenic:Gm8349-Arhgef26 4:intergenic:Gm16057-Dpp6 4:intergenic:Gm24343-Gpr126 4:intergenic:Mrps6-Kcne2 4:intergenic:Gm22438-Gm22159 4:intergenic:Myo5a-Gm9719 4:intergenic:Ermard-Gm5091 4:intergenic:Gm4149-Gm23012 4:intron:E130114P18Rik 4:exon:Prrg3 3:intergenic:Rps6ka2-Tcp10a 3:intergenic:Sod2-Gpr31b 3:intergenic:Gm9992-Gm25872 3:intergenic:Gm26500-Tcp10c 4:intergenic:Gm11901-Gm25173 4:intron:Trp53bp2 3:intron:Cubn 3:intergenic:Gm8609-Gm22543 3:intergenic:Cry1-AC100386.1 4:intergenic:Fut10-Gm23539 4:intergenic:Asic2-4930527B05Rik 4:intergenic:Ppp1r3a-Foxp2 4:intergenic:AC201846.1-Gm20910 4:intergenic:Gm21756-Gm20838 4:intergenic:Gm21943-Gm21724 4:intergenic:Gm21779-Gm21247 4:intergenic:Gm21758-Gm21843 4:intergenic:Gm21756-Gm20838 4:intergenic:Gm21838-Gm21803 4:intergenic:Gm21737-1700040F15Rik 4:intergenic:1700040F15Rik-Gm20794 4:intergenic:Gm21774-Gm21805 4:intergenic:Gm8521-Gm20918 4:intergenic:Gm21805-Gm21241 4:intergenic:Gm21756-Gm20838 4:intergenic:Gm21679-Gm21529 4:intergenic:Gm21918-Gm21832 4:intergenic:Gm21853-Gm21679 4:intergenic:Gm21737-1700040F15Rik 4:intergenic:Gm21744-Gm21094 4:intergenic:Gm21751-Gm21271 4:intergenic:Gm21405-Gm21861 4:intergenic:Gm21241-Gm21739 4:intergenic:Gm21256-Gm21170 3:intergenic:Lpgat1-Nek2 4:intergenic:Arhgap11a-Gm21596 4:intergenic:Gm14788-Mageb2 4:intron:Dclk1 4:intergenic:Gm9001-Gm22283 4:intergenic:Tln2-mmu-mir-7241 4:intergenic:E330013P04Rik-D19Ertd737e 4:intergenic:4931408C20Rik-n-R5s209 4:intergenic:Gm14563-Gm14561 4:intergenic:Gnal-Gm23512 4:intergenic:Gm23727-Gm20594 4:intergenic:Gm24910-Gm25784 4:intron:A830018L16Rik 4:intergenic:Tmed3-Ankrd34c 4:intron:Prex2 4:intergenic:Best3-Lrrc10 4:intergenic:Ahr-Agr3 4:intron:Arnt2 4:intergenic:Gm12152-Pwwp2a 4:intergenic:Gm11259-Gm11256 4:intergenic:Gm25169-Efnb2 3:intron:1700019D03Rik 3:intergenic:Gm26653-Gm26105 4:intron:Fgf14 4:intron:Col6a3 4:intron:Anp32b 4:intergenic:Pea15b-Gm22011 4:intergenic:A730046J19Rik-Gm8199 4:intergenic:Gm23225-Gm24127 4:intron:Cpsf3 4:intergenic:Gm21614-Gm23249 4:intergenic:Gm5576-Reg3b 4:intron:Luzp2 4:intergenic:Gm22032-Gm22895 4:intergenic:Slc30a10-Lyplal1 4:intergenic:Gm25454-n-R5s165 4:intron:Col9a1 4:intergenic:Mycn-Gm24208 3:intron:Brpf3 4:intron:Ccne1 4:intron:Dtd1 4:intergenic:Gm12130-Gm23520 3:intergenic:Npbwr1-Rb1cc1 4:exon:Scai 3:intron:Cfi 4:intergenic:Gm23848-Trps1 4:intron:4930555F03Rik 4:intergenic:Gm13974-4930528P14Rik 4:intergenic:Gm26674/Sash1-Gm9930 4:intergenic:Slc22a23-Pxdc1 4:intergenic:Rad51b-Zfp36l1/Gm26669 3:intergenic:Gm11259-Gm11256 2:intergenic:Olfr51-Olfr54 4:intergenic:Gm22308-Cbfa2t2-ps1 4:intron:Fgfr2 4:intron:Rgs12 4:intron:Gm7271 4:intron:Frmd4b 4:intergenic:Gm26028-Gm23576 4:intron:Hnrnpl 2:intergenic:Gm22906-Cab39 3:intergenic:Gm26275-Arsj 4:intergenic:Gm15975-Gm23896 4:intergenic:Csmd1-Gm25665 4:exon:Slc9a5 4:intergenic:Pik3r1-Gm25808 4:intergenic:Cpeb2-C1qtnf7 4:intergenic:Gm12291-Gm12292 4:intron:Itga11 4:intergenic:Gm15663-Mettl25 4:intergenic:Nucb2-n-R5s157 4:intergenic:Gm13660-Gm13655 4:intergenic:Gm4487-Gm24073 4:intergenic:Cenpw-Gm22623 4:intron:Otc 3:intergenic:Olfr46-Olfr61 4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Gpn1-Supt7l 4:intron:Phf3 Off-target primers show all... show less...
155 / fw	`AAGTCTTAACACTTTTCAGT TGG` `.................... ...` Not with U6/U3 Cloning / PCR primers	64	84	49	26	63	78	0 - 0 - 2 - 21 - 164 0 - 0 - 0 - 2 - 3 187 off-targets	4:intergenic:Usp15-Usp15/AC158802.1 3:intergenic:Gm12141-Gm12142 2:intron:Bcas3 4:intron:Lrrc4c 4:intergenic:Olfr355-Olfr356 3:intergenic:Gpr149-Gm22433 3:intergenic:Gm21614-Gm23249 4:intergenic:Hs3st1-Gm7816 3:intergenic:Capza3-Gm24174 4:intergenic:Gm22913-Gm20091 4:intergenic:Adam23-Dytn 4:intergenic:Gm11395-Vmn2r-ps94 4:intergenic:Gm11392-Gm11394 4:intergenic:Bre-Gm10463 4:intergenic:AC121988.1-Gm25740 4:intergenic:mmu-mir-6393-Zdhhc25 4:intergenic:Gm26054-Xylt1 4:intergenic:Gm13852-Chchd3 4:intergenic:Gm25348-Gm24730 4:intergenic:Gm21833-Rpl21-ps5 4:intergenic:Gm9008-Ctnna2 4:intron:Rc3h2 3:intergenic:Gm15155-Gm15160 4:intron:4921504A21Rik 4:intergenic:Rab27b-Dynap 4:intergenic:Ermn-Gm13534 4:intron:Erbb4 4:intron:Ccdc132 4:intergenic:Gm15024-Tspan32 4:intergenic:Gm14574-Gm14576 4:intron:Ntng1 4:intergenic:Gm11742-Ube2o 3:intergenic:Gm26028-Gm23576 4:exon:Marc2 4:intergenic:1700017N19Rik-Gm26278 4:intergenic:Skint3-Skint9 3:intron:Akap12 3:intergenic:Gm22797-Robo1 4:intergenic:Cttnbp2-Gm23648 4:intron:Zfp644 4:intron:Prune 4:intron:Sntg1 4:intron:Upf2 4:intron:Limch1 4:intron:Gm14061 4:intergenic:Gm23061-Ostf1 4:intergenic:B4galt1-Gm12397 4:intron:Pcbp3 4:intergenic:Fam170b-Vstm4 4:intergenic:Gm23225-Gm24127 4:intergenic:Nrg3-Gm20642 4:intron:Tepp 4:intergenic:Fscb-Gm24066 4:intergenic:Ccbe1-Ccbe1/Gm15958 4:intergenic:Ube2e3-Gm14466 4:intron:Akr1e1 4:intron:Gpr50 4:intron:4931423N10Rik 4:intergenic:Smad3-Smad6 4:intergenic:Enah-Srp9 4:intergenic:AC161571.1-Gm25956 4:intergenic:Gm2464-Ralyl 4:intron:Rnf165 4:intergenic:Gm16477-Gpr26 4:intron:Gm4794 4:intergenic:Gm13481-Gm13489 4:intergenic:Chst11-1700025N21Rik 4:intergenic:Gm26055-Gm22246 4:intergenic:A330048O09Rik-Zfp169 2:intergenic:Rbbp6-Tnrc6a 4:intron:Cyp39a1 4:intergenic:D130040H23Rik-Gm10033 4:intergenic:Gm7790-Gm14977 4:intron:Gm13941 4:intergenic:Ube2e2-Gm20699 3:exon:Arhgap6/Hccs 4:exon:Mrpl15 4:intergenic:Cadm2-Gm15828 4:intron:Cnga1 4:intron:Epha6 4:intergenic:Gm8526-Edil3 4:intergenic:Gm25388-Galnt5 4:intron:Gm11884 4:intergenic:Gm11911-Gm11907 4:intergenic:mmu-mir-6341-Gm2383 3:intergenic:Zkscan2-Gm23788 4:intergenic:Gm25437-Gm22684 4:intron:Usp9x 4:intergenic:Gm15677-Chst15 4:intron:Fam198b 4:intergenic:Tceb2-Prss33 4:intron:Sdk2 4:intergenic:9530036O11Rik-Rnf32 4:intergenic:Gm22565-Gm24288 4:intergenic:Gm7790-Gm14977 4:intergenic:Mmp20-Mmp7 4:intergenic:Zfp607-1700049G17Rik 4:intergenic:Frmd3-Gm11405 4:intergenic:A830009L08Rik-Ssbp2 4:intergenic:Ankrd1-Pcgf5 4:intron:Lphn3 4:intergenic:Grik1-Cldn17 4:intron:Hmcn1 3:intergenic:Pclo/Gm26918-Pclo 4:intron:Agk 3:intron:Nepn 4:intergenic:Gm22482-Rnls 4:intergenic:Gm10601-Gm21966 4:intron:Uxs1 4:intergenic:Prkd1-Gm22088 4:intergenic:Hmgb1-ps2-Gm15061 4:intergenic:Gm22939-Cntnap2 4:intergenic:2010109A12Rik-Ccng2 4:intron:Dpp3 4:intron:Lsamp 3:intron:Pum1 4:intron:Clock 4:intergenic:Ces1h-Ces5a 4:intergenic:Gm13804-Gm13806 4:intergenic:Gm16311-Dab2 4:intron:Myo5a 4:intergenic:Irx5-Irx6 3:intron:Nckap5 4:intergenic:Chst9-Gm10036 4:intron:Ctnna3 4:intergenic:Gm26844-Gm4812 4:intron:Strc 4:exon:Fam160a1 4:intergenic:Agap1-Gm25180 4:intergenic:Gm24295-Mef2c 4:intergenic:Pvrl3-Gm6912 4:intergenic:Pafah1b1-Gm24211 4:intron:Pde4d 4:intergenic:Irx5-Irx6 4:intron:Pid1 4:intron:Ints2 4:intergenic:Gm13976-3110099E03Rik 4:intergenic:Gm22283-Gm9008 4:intergenic:Pvrl3-Gm6912 4:intergenic:Vps13c-Gm22382 4:intron:Fam135b 4:intergenic:A930003A15Rik/AC164558.1-Klhl6 4:exon:Xpo4 4:intergenic:Olfr538-Olfr46 4:intron:2210408I21Rik 4:intron:Smad2 4:intergenic:Gm25935-Gm22593 4:intergenic:Gm22353-Ubr5 4:intron:Astn2 4:intergenic:Sema6a-Gm5506 4:intron:Rsph9 4:intergenic:Gm23529-Ferd3l 4:intron:Tmem117 4:intergenic:AC175117.1-Hnf4g 4:intergenic:Gm25658-Atp2b1 4:intergenic:Gm649-Gm14805 4:intergenic:Gm22828-Gm23960 4:intron:Npsr1 3:intron:Unc13c 3:intron:Vdac2 4:intergenic:Tmtc1-Gm15763 4:exon:Epb4.1l5 3:intron:Stx8 4:intergenic:Zfp715-Siglec5 4:intron:Ndrg3 3:intergenic:Ahctf1/Gm10518-Cdc42bpa 4:intergenic:4933440J02Rik-Krr1 4:intron:Gnl2 4:intergenic:Gm14915-Gm14909 4:intron:Pdlim2 4:intron:Camta1 4:intron:Dync2h1 4:intergenic:Pten-Gm22482 3:intergenic:5S_rRNA-Gm22503 4:intergenic:Hdac4-Ndufa10 4:intergenic:Gm13988-Sema6d 3:intron:Gm15245 3:intergenic:Gm25264-Gm25959 4:intergenic:Gm11234-Tle1 4:intron:Brpf3 4:intergenic:Tenm3-Gm25830 4:intergenic:Gm23558-Galc 4:intron:Spink2 4:intron:Hepacam2 4:exon:Gm11842 4:intergenic:Hpgds-C130060K24Rik 4:intron:Aak1 Off-target primers show all... show less...
203 / fw	`CTGACTGGTGGCTGTAAGGG AGG` `.................... ...` Cloning / PCR primers	61	84	49	74	65	74	0 - 1 - 3 - 42 - 194 0 - 0 - 0 - 1 - 3 240 off-targets	4:intron:Ptbp2 4:intergenic:mmu-mir-6540-Gap43 4:exon:Golga7b 4:intergenic:Pigt-Gm14317 4:intergenic:RP24-480M3.2-RP23-175E16.1 4:intron:5730522E02Rik 4:intron:Mcf2l 4:intergenic:Tlr5-Disp1 3:intergenic:Cpa6-Gm15603 3:intron:Map2k5 4:intergenic:Gm26190-B3gat1 4:intergenic:Rab12-Themis3 4:intergenic:mmu-mir-6393-Zdhhc25 3:intergenic:Fut8-Gm24070 3:exon:Abcd1 4:intergenic:Rassf10-Gm23662 4:intergenic:Rnf144b-4931429P17Rik 4:intron:Shisa6 2:intron:Fcer2a 4:intron:Apba1 4:exon:Pkig 4:intron:Sobp 3:intergenic:Ceacam12-Igfl3 4:intergenic:Gm1965-Gm6507 4:intergenic:Gm12011-Vstm2a 4:intergenic:Veph1-Shox2 3:intron:Cyp27a1 4:intergenic:1700006A11Rik-Gm22900 4:intergenic:RP23-341H6.1-Lca5 4:intergenic:A430010J10Rik-Gm8566 4:intron:2010300C02Rik 4:intron:Gria3 2:intergenic:5S_rRNA-Cops8 4:intron:Wdr7 4:intron:4930564G21Rik 4:intergenic:Mrps22-PISRT1 4:intergenic:Olfr76-Olfr1431 3:exon:Cdhr1 4:intron:Ica1 4:intergenic:1700112E06Rik-Gm5670 4:intron:Gm805 3:intergenic:Kcnt2-Gm4845 3:intergenic:Gm12789-Gm12799 4:intergenic:Gm26256-n-R5s61 3:intergenic:Csmd2-Hmgb4 4:intergenic:Arhgap6-Amelx 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 3:intron:Atp6v1h 4:intergenic:Gm24354-Ptprn2 4:intergenic:A1bg-Gm24696 4:intergenic:Gm24258-Bnip3l 4:intergenic:Nucks1-Slc45a3 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Atp6v1h 4:intron:Hivep3 4:intron:Shisa6 4:intron:Adamtsl3 4:intergenic:Gm26233-Cttnbp2 4:intron:Kcnn3 4:intron:Mki67ip 4:intergenic:Fibcd1-Gm13441/Lamc3 4:intergenic:Gm25985-Gm6404 4:intergenic:Vipr2-Gm23732 2:intergenic:Pdlim2-Sorbs3 4:intron:Copg1 4:intergenic:Dock5-Nefm/Nefl 3:exon:Pitpnm1 4:intergenic:Baz2b-Gm13572 3:intergenic:Gm24382-Mbnl1 3:intron:Slc6a1 4:intron:Trp73 4:intergenic:Gm22032-Gm22895 4:intergenic:Gm11508-2010015M23Rik 4:intergenic:Gm24696-Myc 4:intron:Nid2 4:intergenic:Gm24584-Sfrp2 4:intron:Stard8 4:intergenic:Gm13484-Gm13498 4:intergenic:Prelp-Fmod 3:intergenic:Gm11241-Ptprd 1:intergenic:Irx5-Irx6 4:intron:Olfr272 4:intergenic:AC161214.1-Gm11033 4:intron:Slc6a6 4:intergenic:Mmd-Hlf 4:intergenic:Gm11765-Gm11411 4:intron:Ttll11 4:exon:Dennd4b 4:intron:Sept9 3:intergenic:Neu2-Inpp5d 4:intergenic:Bre-Gm10463 4:intron:Asic2 3:intergenic:Abcg2-Herc3 3:exon:Dtx1 4:intron:Ptprf 4:intergenic:Trav16d-dv11-Trav13d-4 4:intron:Morn1 4:intergenic:Spp2-Glrp1 4:intergenic:Gm24790-Klf6 4:intergenic:Olfr1382-Olfr1381 4:intron:Rpp14 4:intron:Cldn14 4:intergenic:Vmn1r123-Vmn1r124 4:intergenic:Vmn1r132-Gm4175 4:intergenic:Vmn1r118-Vmn1r119 4:intergenic:Gm4498-Gm4133 4:intergenic:Vmn1r160-Gm4214 4:intergenic:Gm4567-Vmn1r157 4:intron:Nlrp9b 4:intron:Vmn1r139 4:intergenic:Gm10668-Vmn1r112 4:intergenic:Gm8453-Gm4558 4:intergenic:Gm4172-Vmn1r132 4:exon:Gm498 4:intron:Ift43 4:intron:Slc26a5 4:exon:Serhl 4:exon:Sik1 4:intergenic:Gm23534-Gm22727 4:intergenic:U7-Gm11987 4:exon:Lrp3 4:intron:Cdc42bpb 4:intron:Pde6d 4:intron:Gm684 4:intergenic:Asxl2-Dtnb 4:intergenic:Gm14970-4930412D23Rik 3:intergenic:Gm16314-Gnb1l 4:intergenic:Gm23821-Tgfb2 3:intergenic:Gm5839-Gm25440 4:intergenic:Gm22911-Rfpl4b 4:intergenic:n-R5s82-Pknox2 4:intergenic:Gm24341-Cnr1 4:intergenic:Nrbp2-BC024139 4:intron:Tmco4 4:exon:Cln6/Gm10653 4:exon:Adra2b 4:intergenic:Gm25000-Dapk1 4:intergenic:Tle3-Rplp1 4:intron:Rnf150 3:intergenic:Zfp592-Alpk3 3:intergenic:Cdh7-Cdh19 3:intergenic:Gm6594-Pkdcc 3:intergenic:Spc24-Kank2 4:intron:Tusc3 4:intergenic:Gm24936-Eif4e3 4:intron:Syt9 4:intergenic:Cgnl1-RP23-79K5.1 4:intron:Scube1 4:intron:Klhl30 4:intergenic:Fam189a2-1700021P04Rik 4:intergenic:BC034090-Xpr1 4:intergenic:Trib1-Gm7083 4:intergenic:Zhx2-Derl1 4:exon:Atg16l1 4:intron:Apbb2 4:intergenic:Gpatch2-Esrrg 4:intergenic:RP23-339G10.1-Unc13c 4:intergenic:Crygf-Gm8809 4:intergenic:Gm24404-Nrg3 4:intron:Dpysl2 4:intergenic:Dock4-Immp2l 4:exon:Fgfr3 4:exon:Ttbk2/Cdan1 4:intergenic:Sipa1l2-Map10 4:intergenic:Gm25265-Prex1 4:intergenic:Gm25402-Dennd2a 4:intron:Foxp1 4:intergenic:Nhp2l1-4930407I10Rik 4:intergenic:Gm4906-Gm14515 4:intergenic:Gm20646-Mrps9 4:intron:Tspan8 4:intron:Kcnh6 4:intergenic:Phyhipl-4930533K18Rik 4:intergenic:Pdik1l-Trim63 4:exon:Matk 4:intergenic:Hmgb1-ps4-2410152P15Rik 4:intergenic:Pdcd1-Gm23389 4:intergenic:Mir3098-Vamp1 4:intron:Aldh1l1 4:intron:Fer1l5 4:exon:Igfn1 4:intergenic:Gm14747-Fam47c 4:intron:Abtb2 4:intergenic:En1-B230209K01Rik Off-target primers show all... show less...
204 / fw	`TGACTGGTGGCTGTAAGGGA GGG` `.................... ...` Cloning / PCR primers	52	81	63	72	65	67	0 - 1 - 6 - 24 - 230 0 - 0 - 1 - 2 - 3 261 off-targets	4:intron:Tenm3 4:intergenic:SOX2OT_exon4/Sox2ot-Sox2ot 4:exon:Hoxa3 3:intergenic:Ceacam12-Igfl3 4:intergenic:Gm23959-Gm12016 4:intergenic:Gm25769-Gm11487 3:intron:Sil1 4:intergenic:AC158942.1-5031425E22Rik 4:intron:Msh3 4:intergenic:Gm23389-Gm23181 4:intergenic:Camk2d-Gm15551 4:intergenic:Gm24382-Mbnl1 4:intron:Whrn 4:intergenic:Gm5432-Lpin1 4:intron:Lmf1 3:exon:Pitpnm1 4:intergenic:Celf2-Gm24340 4:intergenic:Gm9192-Gm23735 4:intron:Cachd1 4:intron:Tspan5 4:intron:Arhgap23 4:intron:Otogl 4:intergenic:Gm25583-Macrod2 4:intergenic:Olfr76-Olfr1431 1:intergenic:5S_rRNA-Cops8 4:intergenic:Gm11765-Gm11411 4:intron:Aff1 3:intergenic:Gm24354-Ptprn2 4:intergenic:Cebpa-Slc7a10 3:intergenic:Gm12369-Lingo2 4:intergenic:Ak3l2-ps-8430437L04Rik 2:intergenic:Gm25292-Gm26667 4:exon:Gm20631/Hmcn1 4:intron:Apbb2 3:intron:Arhgap22 4:exon:Alkbh8 4:intron:Tspan14 4:intergenic:Ranbp6-Il33 4:intron:Setd4 4:intergenic:Gm24150-Gm17359 4:intergenic:Smad3-Smad6 4:intergenic:Gm25560-Wdr64 4:intergenic:Smlr1-Tmem200a 4:intergenic:Cd2ap-Tnfrsf21 4:intron:Prom1 4:intergenic:Gm25598-BC002059 4:intergenic:Rbpsuh-rs3-Cntnap2 3:intergenic:Fosl2-Gm26335 4:intergenic:Usp17ld-Gm25171 4:intergenic:RP24-242F4.8/Zfp456-Zfp429 3:intergenic:Neu2-Inpp5d 4:intergenic:Olfr816-Olfr818 4:intergenic:Agbl1-Gm23239 4:intergenic:Sema6a-Gm5506 4:intergenic:Wac-Gm5819 4:intron:Trpm1 4:intergenic:Sh3bgrl3-Cep85 4:intron:Gpc6 4:intron:Ptprn2 4:intron:Hivep3 4:intron:Grm8 4:intergenic:Gm11721-Sap30bp 4:intron:Chn1 4:intron:Zbtb21 4:intron:Ust 4:intron:Mcf2l 4:intron:Zc3h12c 4:intergenic:Gm9630-Gm24080 4:intergenic:Gm24587-Dcn 4:intergenic:Gm22827-Gm22181 3:intron:Syt9 4:intergenic:Mrps22-PISRT1 4:intergenic:Gm26137-Gm23733 4:intergenic:Tle4-Gm26026 4:intergenic:Gm26106-Gm7729 4:intergenic:Pcbd1-Sgpl1 4:exon:Zfp740 4:exon:Ttll9 4:intergenic:Gm11599-Nrp2 4:intron:Rnf150 4:intergenic:Kcnh8-Efhb 4:exon:Myom1 4:exon:Fstl4 4:intergenic:Dock5-Nefm/Nefl 4:exon:Bean1 4:intron:Garem 3:intergenic:Trib1-Gm7083 4:intron:Elmo1 4:intergenic:Ccnjl-Gm12150/Ccnjl 4:intron:Apbb2 4:intron:Ptprn2 3:intron:Hspb8 4:exon:Fam109a 3:intron:Ptprf 4:intergenic:Gm23152-Gm25265 4:intron:Scube1 4:intergenic:Pak4/Samd4b-Papl 4:intergenic:Vmn1r231-Vmn1r232 4:intergenic:Glod4/Vps53-Vps53 4:intron:Celsr1 4:intergenic:Gm10784-Gm17611 4:intergenic:Ola1-Sp9 4:intron:D6Ertd474e 4:intergenic:Gm13065-Gm13064 4:intergenic:Gm17538-Gm5229 4:intron:Sipa1l2 3:intergenic:Spc24-Kank2 4:intergenic:Gm25990-Cyp2d9/AC113593.2 4:intron:Cped1 4:intergenic:Krt74-Krt72 4:intergenic:Luzp2-Gm26482 4:intergenic:Gm23538-1700110M21Rik 4:intergenic:Fam20a-Gm11684 2:intergenic:4933405E24Rik-Csf2/Gm12223 4:intron:Rpp14 4:intergenic:Gm10257-Gm6114 4:intergenic:4930578C19Rik-Gm6923 4:intergenic:Zdhhc14-3300005D01Rik 4:intron:Pmepa1 4:intergenic:Jazf1-Gm25818 4:intron:Pdcd1lg2 4:intron:Chchd6 4:intergenic:Psg29-Gm24520 4:intergenic:Psg29-Gm24520 4:intergenic:Fam49a-5S_rRNA 4:intergenic:Gm23378-Gm22266 4:intergenic:Igsf11-Gm24795 4:intergenic:Cntn5-Gm25365 4:intron:Copg1 3:intergenic:1700112E06Rik-Gm10248 4:intergenic:Baz2b-Gm13572 4:intergenic:Wfdc12/A730032A03Rik-Wfdc12 4:intron:Shank1 4:intron:Csnk1a1 4:intron:Mgat5 4:intergenic:Tnfsf13b-Myo16 3:intergenic:Gm25700-Gm24935 4:intergenic:Chmp2b-Vgll3 4:exon:Il34 3:intron:Adcy9 4:intron:Zdhhc14 4:intergenic:Osbpl9-Calr4 4:intron:Garem 4:exon:Pkd1 4:intron:Telo2 4:intron:Zfp236 4:intergenic:Gm22032-Gm22895 4:intergenic:Cdkal1-E2f3 4:intergenic:Dock4-Immp2l 4:intron:Slc4a4 4:intergenic:Gpr149-Gm22433 4:intergenic:Fibcd1-Gm13441/Lamc3 2:intergenic:Gm24839-Tmem132c 4:intron:Pdlim1 4:intergenic:Pabpc2-Yipf5 4:exon:Naglu 4:intergenic:Gm25115-Diap3 4:intron:Gm12580 4:intergenic:Stk36-Ttll4 3:intron:Rab3b 3:exon:Gm4189 4:intergenic:Gm8897-Gm8906 4:intergenic:Gm6460-Gm8922 4:intergenic:Gm6465-4933402N22Rik 4:intergenic:Gm6455-Gm8857/Gm8871 4:intergenic:Gm8926-Gm6465 4:intergenic:Speer1-ps1-Gm8897 4:intergenic:Gm8922-Gm8926 4:intergenic:Gm8890-Speer1-ps1 4:intergenic:Gm5861-Gm8890 4:intergenic:Gm14747-Fam47c 4:intergenic:BC034090-Xpr1 4:intergenic:Gm25402-Dennd2a 4:intergenic:Mir3098-Vamp1 4:intergenic:Nhp2l1-4930407I10Rik 4:intergenic:Gm20646-Mrps9 4:intergenic:Hmgb1-ps4-2410152P15Rik 4:intergenic:Gm4906-Gm14515 4:intergenic:RP23-339G10.1-Unc13c 4:intron:Scube1 4:intergenic:Ildr2-Tada1 2:intron:D030068K23Rik 4:intron:1110004E09Rik 4:intron:Nckap5 4:intergenic:Gm14487-Cstad 2:intergenic:Gm23061-Ostf1 3:intron:Cyp27a1 3:intergenic:Cpa6-Gm15603 4:intergenic:Pdcd1-Gm23389 4:intron:Nostrin 3:intergenic:Gm24258-Bnip3l 2:intergenic:Irx5-Irx6 3:intron:Slc6a6 3:intergenic:Gm23916-Osbpl11 3:intergenic:Tex19.1-Uts2r 4:intergenic:PVT1_3-Ndufa4 4:intergenic:Spp2-Glrp1 4:intron:Atp13a5 4:intergenic:Gm25702-Gm5473 4:intergenic:Tnrc6b/Mir5113-Adsl 4:intergenic:Gm25813-Gm23666 4:intergenic:Gm22807-Gm23681 4:intron:Nsmce2 4:intron:Mapre2 4:intergenic:Igsf21-Gm9867 4:intron:Mipep 4:intergenic:Gm27039-Adcyap1 4:intergenic:mmu-mir-6393-Zdhhc25 4:intergenic:Gm14285-4930425F17Rik 4:intron:Pde6d 4:intergenic:Gm25485-Gm12460 4:intergenic:Cnpy1-Rbm33 4:intron:Tmem65 4:intron:Ldb2 4:intergenic:Rpl31-ps15-Gm26404 4:intergenic:Fbln1-Atxn10 4:intergenic:Sipa1l2-Map10 4:intergenic:Gm3222-Gm23352 4:intron:Gabrg3 4:intron:Bmp7 4:intron:Sqstm1 4:intergenic:n-R5s82-Pknox2 4:intergenic:Gm8217-Htr5b 4:exon:Setbp1 4:intergenic:D230002A01Rik-Gm24254 4:intergenic:Tbc1d5-Satb1 4:intron:Col2a1 4:intergenic:Gm23534-Gm22727 4:intron:Cldn14 4:intergenic:Gm11680-BC006965 4:intergenic:Gm5334-Arrdc4 4:intergenic:St6galnac3-Gm10042 4:intergenic:Gm26883-Gm26883/Tshz2 4:intergenic:Gm26682-Zfp438 4:intergenic:Gpihbp1-Zfp41 4:intron:P2ry2 4:intron:Cwc27 4:intron:Trio 4:intron:Sin3b 4:intron:Lrrc2 4:intron:Col4a1 4:intergenic:Igkv1-131-Igkv14-130 4:intron:Lonrf1 4:intron:Aff1 4:intron:Gabrg3 4:exon:Zfp398 4:intergenic:Gm24720-Tshz1 4:intron:Morn1 4:intergenic:Gm8601-Btg1 4:intron:Mroh2a 4:intron:Gm12962 4:intron:Trerf1 4:intron:Ncor2 4:intergenic:Fam115a-Olfr453 4:intron:Ntm 4:intron:Tgfbr2 4:intergenic:Gm6091-Gm26759 4:intergenic:Gm22906-Cab39 4:intron:Stard7 4:intron:C130046K22Rik 4:intergenic:Gm22319-Adam7 Off-target primers show all... show less...
47 / rev	`CACATTTATAAATGAGACAA AGG` `.................... ...` Cloning / PCR primers	49	64	61	46	68	73	0 - 0 - 8 - 68 - 379 0 - 0 - 3 - 7 - 12 455 off-targets	4:intron:Sarm1 4:intron:Tenm3 4:intergenic:Mia2-Ctage5 3:intergenic:Fscb-Gm24066 4:intergenic:Rgs1-Rgs21 3:intergenic:Gm12602-Mtap 4:intergenic:Gm22369-Incenp 4:intergenic:Ighv12-3-Ighv6-3 3:intron:Rdh11 4:intron:Pcdh15 4:intergenic:Gm24632-Lyzl1 4:intron:Ssh2 4:intergenic:Gm22537-Sp8 3:intergenic:Pigyl-Rpl15-ps3 4:intergenic:Gm24987-Socs6 3:intergenic:Rnase13-Arhgef40 4:intergenic:Gm4929-Fam84a 4:intergenic:Gm17538-Gm5229 4:intergenic:Gm6564-AC158942.1 4:intergenic:Nr3c1-Pabpc2 4:intergenic:Rgs8-Rgs16 4:intergenic:Gm16391-Gm17538 3:intergenic:Gm23763-Gm25609 4:intergenic:Grxcr1-Gm23605 4:intergenic:Gm23727-Gm20594 4:intron:Ttll5 4:intergenic:AC113989.1-Gm24780 3:intergenic:Gm23728-Foxb1/B230323A14Rik 4:exon:Nedd4l 4:intergenic:Pds5b-Gm24630 4:intron:Gm11867 3:intergenic:Kcnq3-RP23-389M1.2 4:intergenic:Kcns3-Msgn1 3:intron:Mgam 3:exon:Gm26930 4:intron:Mospd2 4:intergenic:Ubqln3-Ubqlnl 2:intron:A430033K04Rik 3:intergenic:Emid1-Kremen1 4:intergenic:Gm26006-Gm26139 4:intergenic:Gm24880-Gm10172 4:intron:Pde8b 4:intron:Mpp2 4:intergenic:Drr1-Gm14520 2:intron:Tgfbr3 4:intergenic:Gm25977-Gm11875 4:intergenic:Cnga2-Magea4 4:intergenic:Gm22917-Gm26245 4:intron:Hecw1 4:intergenic:Gm26006-Gm26139 4:intron:Kidins220 4:intergenic:Gm27039-Adcyap1 4:intergenic:Gm22628-5033411D12Rik 4:intron:Rab8b 4:intron:Fhit 4:intron:1700001L05Rik 4:intergenic:Ccdc42-Myh10 4:intergenic:Gm24444-Mgmt 4:intergenic:Cdh26-Gm14314 3:intergenic:Cpn1-Cyp2c44 4:intergenic:Gm14930-Pcdh11x 4:intergenic:Slc22a26-Slc22a27 4:intergenic:Igkj5-Igkc 4:intron:Zfp385b 3:intergenic:Tbc1d21-4930461G14Rik 3:intergenic:Tbc1d21-4930461G14Rik 3:exon:Cacnb4 4:intergenic:Gm23993-Adam22 4:intron:Tec 4:intergenic:Wdr49-Pdcd10 4:intergenic:4930529I22Rik-Gm14249 4:intergenic:5033403F01Rik-Gm23972 3:intron:Foxp1 4:intergenic:Gm7931-Slit2 4:intron:Ankrd6 3:intergenic:Gm8825-Gm25176 3:intergenic:Gpr6-Fig4 4:intergenic:Csl-Kitl 4:intron:Thsd4 4:intergenic:Tbc1d21-4930461G14Rik 4:intergenic:Lgr5-Tspan8 4:intergenic:Htr3a-Htr3b 4:intergenic:Sgcz-Gm16204 4:intron:Dkc1 3:intron:Map4 4:intergenic:Lrrn3-Immp2l 3:intergenic:5033403F01Rik-Gm23972 4:intergenic:Ocrl-Apln 4:intron:Chm 3:intergenic:Asap1-Adcy8 4:intergenic:Ppp1r2-ps9-Gm14519 4:intron:Tgfb1i1 4:intergenic:Gm24774-Pcdh17 4:intergenic:1700034J05Rik-Ppfibp1 4:intron:Nup205 4:intergenic:Gm15501-Gm5899 4:intron:L3mbtl4 4:exon:Srsf10 4:intergenic:Unc93a-Smok2a 4:intergenic:Rnu6-Tcte2 4:intergenic:Gm25015-Gm7481 4:intergenic:Rassf9-Alx1 2:intron:Msrb3 4:intergenic:Fam220a-Cyth3 4:intergenic:Robo1-Gm25699 4:intergenic:Cenpv-Gm12279 3:intergenic:1700092E19Rik-Gm11353 4:intergenic:Runx3-Clic4 4:intergenic:Pon2-Asb4 4:intron:Phf16 4:intergenic:Zfy2-Gm22119 4:intron:Fam49a 4:intergenic:Slitrk1-Gm25901 4:intergenic:PCGEM1-Gm25843 4:intergenic:Spry3-Tmlhe 4:exon:Fbln2 4:intergenic:Mirlet7a-2-2610203C20Rik 4:intergenic:Gm24870-Srek1 4:intergenic:Gm22845-Nt5c1b 3:intergenic:Gm15128-Gm15081 3:intergenic:Gm7155-Gm8692 3:intergenic:Gm15094-Gm15096 4:intergenic:Gm24896-Casp1 4:intergenic:Rgs1-Rgs21 4:intergenic:Gm25650-Gm23584 4:intergenic:Gm22506-RP23-448C3.1 3:intron:Car12 4:intergenic:Xkr6-Mir598 4:intergenic:Cadm2-Gm24681 3:intron:9530026P05Rik 4:intergenic:Amn1-2810474O19Rik 4:intergenic:Vdac3-Dkk4 4:intron:Fhit 4:intron:Gm20732 4:exon:Fam194a 4:intergenic:Gm7670-1700001G01Rik 4:intergenic:Gm26480-Gm6863 4:intron:E130308A19Rik 3:intergenic:Scamp5-Gm6166 4:intergenic:D130040H23Rik-Gm10033 4:intergenic:Gm24812-Gm14774 4:intron:Klhl1 4:intergenic:Zkscan2-Gm23788 4:intergenic:Olfr1397-ps1-Cep112 3:intergenic:Gm9742-Adarb2 4:intergenic:Gm11498-Gm22702 4:intergenic:Lins-Cers3 3:intergenic:Gm23056-Thsd7b 4:intergenic:Gm8707-Sorl1 4:intron:Angptl2 4:intergenic:Gm23022-Rab28 4:intron:Dicer1 4:intron:Zc3h12b 4:intergenic:Tmem126b-Dlg2 4:intron:Gtf2f2 4:intergenic:5033430I15Rik-Gm10113 4:intergenic:Gm15112-Gm8331 4:intergenic:Gm15090-Gm8346 4:intergenic:Gm15110-Gm8303 4:intergenic:Gm15101-Gm8383 4:intergenic:Gm15113-Gm8320 4:intergenic:Gm15103-Gm8402 4:intergenic:Gm15089-Gm8366 4:intergenic:Gm12443-Anp32b 4:intergenic:Gpr126-Vta1 4:intron:Gad2 4:intergenic:Gas2l3-Nr1h4 4:intergenic:Gm22444-mmu-mir-6238 4:intergenic:Skint9-Gm12819 4:intron:Pepd 4:intergenic:Ppp1r2-ps2-Nup210 4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Gm22726-Gm24388 4:intergenic:Gm24129-Tecrl 4:intergenic:Gm4929-Fam84a 4:intron:Cep120 4:intron:Cab39 4:intron:Glp2r 4:intergenic:Gm23777-Slc16a7 4:exon:Tinag 4:intergenic:Vapb-Gm14371 4:intergenic:AC121538.1-Ncoa2 4:intergenic:Gm24441-Gm24870 4:intergenic:Gm25818-Creb5 4:intergenic:Mir3072/Mirg-Dio3os 4:intergenic:Plekhf1-Pop4 4:intergenic:F930017D23Rik-Gm9034 4:intron:Tfcp2 4:intron:Srd5a3 4:intron:Epsti1 4:intron:Hdac9 4:intergenic:Gm24295-Mef2c 4:intergenic:Gm22503-Gm24271 4:intergenic:Gm13265-Gm13263 4:intergenic:Dpyd-Gm23432 4:intergenic:Ttll7-Gm23131 4:intron:Nrg4 4:intergenic:Olfr1173-Olfr1174-ps 4:intron:Tmprss15 3:intergenic:D230002A01Rik-Gm24254 4:intron:Pdgfd 3:intergenic:Gm23829-Gm5974 4:intergenic:Gm9843-Usp25 4:intergenic:Kif21a-Abcd2 4:intergenic:Gm22934-A530021J07Rik 4:intergenic:Lpp-Mir28 4:intron:Mmd 4:intergenic:5S_rRNA-Cops8 4:intergenic:NAMA_2-Gm22670 4:intergenic:Gm12128-Gm25693 3:intergenic:Slc16a7-Lrig3 4:intergenic:Add2-Tgfa 4:intergenic:Abcc4-Gm23302 4:intergenic:Gm24256-Grik2 3:intergenic:4930558G05Rik-Gm26029 2:intergenic:Lrrc31-Samd7 4:intergenic:Gm5272-1700008P02Rik 3:intergenic:Gm4211-9330111N05Rik/Gm4211 4:intron:Usp47 4:intron:Opcml 4:exon:Snord67/Ckap5 4:intergenic:Ins1-Xpnpep1 4:intergenic:Emcn-Gm4861 4:intron:Sema5a 4:intergenic:Efr3b-Dnajc27 4:intergenic:Gm23013-Ppp1r3a 4:intergenic:Gm24462-C230034O21Rik 4:intron:Itgb8 3:intergenic:Mrpl14-1600014C23Rik 4:intron:Sema3a 4:intergenic:Pcdh20-Gm25886 4:intron:Nup210l 4:intergenic:Ear-ps12-Ang-ps2 4:intergenic:Ang-ps1-Gm16545 4:intergenic:Ear-ps8-Ang-ps3 4:intron:Akap11 4:intergenic:Gm7536-Gm24704 4:intergenic:Gm22343-Ncoa1 4:intergenic:Gm23892-Gm25382 4:intergenic:Gm14461-Gm14460 4:intergenic:Zfp42-Gm22986 4:intron:Nol4 3:intron:Hps5 3:intron:Pgm5 3:intron:Creb3l1 4:intergenic:Eya4-Rps12 4:intron:Rpgrip1l 4:intron:4932438A13Rik 4:intergenic:Gm9578-Gm22891 4:intergenic:Gm16294-Zfpm2 4:intron:Tcl1 4:intergenic:Bpifc-A230060F14Rik/Fbxo7 4:intron:Alas2 4:intergenic:Aldh3b1-Unc93b1 4:intergenic:Adra1b-Gm12153 4:intergenic:Gm5185-Gm24921 4:intron:Slc22a30 4:intergenic:Sgms1/2700046G09Rik-Rpl9-ps6 4:intron:Lrrc42 4:intron:Itgb5 3:intron:Camk2d 2:intron:Adcy7 3:intergenic:Gm23044-Utrn 4:intron:Zfpm2 4:intergenic:Gm21614-Gm23249 4:intron:Grip1 4:intron:Dcc 4:intron:Trim62 4:intergenic:Gm23653-Nnmt 4:intergenic:Mybpc1-Spic 4:intron:Dock1 4:intergenic:Gm23311-Gm16013 4:intron:1300015D01Rik 4:intergenic:4930405D11Rik-Kif2b 4:intergenic:4930474H06Rik-Gm22959 4:intron:Gpc6 4:intergenic:Fbxw7-Mir466k 4:intron:Ebf1 3:intergenic:Gm24298-4921513I03Rik 4:intergenic:Sema6a-Gm5506 4:intron:Elf1 4:intergenic:9330154J02Rik-Tpbg 4:intron:Diap3 4:intergenic:Gm17091-Gm6455 4:intron:Nalcn 4:intergenic:Gm5742-Gm22223 4:intergenic:Mapk1ip1l-Lgals3 4:intergenic:Hmgb1-ps9-Pik3r1 3:intron:Nrp1 4:intron:Dcc 4:intergenic:Vmn2r120-Gm21834 4:intron:Il1rapl1 4:intergenic:Gm9008-Ctnna2 4:intergenic:Hus1-Sun3 4:intergenic:Mrpl36-Lpcat1 4:intergenic:Trh-Prickle2 4:intergenic:Gm26923-Gm25143 3:intergenic:n-R5s30-Rbfox1 4:intergenic:Gm12807-Agbl4 3:intron:Pcdh19 3:intron:Zfand3 4:intergenic:Mbd2-Dcc 4:intergenic:Gm22507-Trhde 4:intergenic:Flrt3-Macrod2 4:intergenic:Gm10620-Cmc2 3:intergenic:Spock3-Gm5350 4:intergenic:Tpd52-Zbtb10 4:intergenic:Gm11890-Gm11892 4:intergenic:Fscb-Gm24066 4:intron:Pcp4/Igsf5 4:intron:Rgs7 4:intron:Ophn1 4:intron:Wdr72 4:intron:Igsf21 4:intergenic:4933413L06Rik-Hcn1 4:intergenic:Rad51b-Zfp36l1/Gm26669 4:intergenic:Gm6133-Gm26430 4:intergenic:Heca-Abracl/Gm17711 4:intergenic:Nxph3-Ngfr 4:intergenic:Gm11997-Vwc2 3:intron:Pde4d 4:intergenic:4930474N05Rik-Gm24394 4:intergenic:Gm10631-Bean1 3:intron:St3gal3 4:intron:Cntn5 4:intergenic:Gm10292-Schip1 4:intergenic:Colec10-Mal2 4:intergenic:Shq1-Gxylt2 4:intron:Eya2 4:intergenic:Sipa1l1-Gm5435 4:exon:Gtf2a1 4:intergenic:Gm14840-Gm3880 4:intron:Auts2 4:intergenic:Zeb2-VIS1 4:intron:Ptprk 3:intron:Ptprd 2:intergenic:Gm10115-Celf2 4:intron:Fancl 4:intergenic:Sox13-Gm23939 4:intergenic:Polg-AI854517 4:intergenic:Kcna4-Gm9864 4:intergenic:Zfp648-n-R5s220 4:intron:Gm13481 4:intergenic:Rarb-Thrb 4:intergenic:Gm26228-Gdf10 4:intergenic:Slc25a12-Gm13641 4:intron:Zfp407 4:intergenic:Gm24522-4930558G05Rik 4:intergenic:Gm14920-Gm14918 4:intergenic:Leprot-Lepr 4:intron:Pphln1 4:intron:Pld3 3:intergenic:Rps15a-ps1-C230021G24Rik/Ppfia2 3:exon:Ubxn7 4:exon:Gm10681 4:intergenic:Gm25388-Galnt5 4:intergenic:Gm9800-Fhit 4:intron:Fam114a1 4:intron:Pgm2 3:intergenic:Epha7-Gm11917 4:intron:Gm11033 4:intergenic:Gm8840-Ikzf2 4:intergenic:Gm11765-Gm11411 4:intron:Atxn7 4:intron:Cnbd1 4:intergenic:Btg3-Gm25038 4:intergenic:Hs6st2-Gm14581 4:intergenic:Gm410-Rnf13 4:intergenic:Cst10-Gm24551 2:intron:Nrxn3 4:intergenic:Dhx35-Gm25090 3:intergenic:Gm10000-Dicer1 4:intergenic:Gm23242-Gm25670 4:intron:2900026A02Rik 4:intergenic:Gm26256-n-R5s61 3:intergenic:Pkig-Gm16316 3:intron:Gm872 4:intergenic:Bbs9-Bmper 4:intergenic:H2-M10.4-Gm20452 4:intron:Prkdc 4:intergenic:A930011G23Rik-Gm16228 4:intergenic:Cbfb-D230025D16Rik 4:intergenic:Gm24737-Lhfpl2 4:intergenic:Gm22506-RP23-448C3.1 4:intron:Stat4 4:intron:Ogt 4:intergenic:Zic4-Gm26101 4:intron:Mtm1 2:intron:Itfg1 3:intergenic:Veph1-Shox2 4:intergenic:Gm16029-Gdnf 4:intron:Mid1 4:intergenic:Gm6586-Gm10482 3:intergenic:Gm5555-Gpr125 4:intergenic:Gm8100-Gm26795 3:intron:Zfp277 4:intergenic:Gm16630-Gm22122 3:intron:Iqch 3:intron:Ykt6 3:exon:Gm4450 3:intergenic:Gm12399-Gm12400 3:exon:Gm10681 3:intron:Clstn1 3:intergenic:3222401L13Rik-Pcdhga9 3:intergenic:Hsd3b4-Gm10681 3:intergenic:Map7d2-A830080D01Rik 4:intergenic:Galnt7-Galntl6 4:intergenic:mmu-mir-6343-Gm25974 4:intergenic:Hsd3b5-Gm12399 4:intergenic:Eif3e-Gm10373 4:intergenic:Gm22044-Zfp827 4:intergenic:n-R5s175-Gm2301 4:intron:Prdm2 4:intron:Crim1 4:intron:Csgalnact1 4:intron:Itga11 4:intergenic:Hsd3b5-Gm12399 4:intergenic:Chrm3-Gm26861 4:exon:Gm2155 4:intergenic:1810037I17Rik-Usp53 4:intergenic:4930550C17Rik-Neurog1 4:intron:Kcnh8 4:intergenic:4921530L21Rik-Klhl1 4:intron:Scd1 4:intergenic:Gm24297-Gm22911 4:intergenic:A830012C17Rik-Gm11780 4:intron:Cenpe 4:intergenic:Gm5529-Gm23934 4:intergenic:Gm19965-Gm24030 4:intergenic:Bri3-Nptx2 4:intron:Grm5 4:intergenic:Mettl21e-Gulp1 4:intergenic:Gm22223-Gm22715 4:intergenic:Cttnbp2-Gm23648 4:intergenic:Gm13217-1700061F12Rik 4:intron:Usp24 4:intergenic:n-R5-8s1-Mex3c 4:intron:Lyplal1 4:intergenic:Rcn1-4930527A07Rik 4:intergenic:Gm23036-Gm22034 4:intron:Fstl5 4:intron:Orc1 4:intron:Card11 4:intron:Mgat5 4:intron:Dtnb 4:intergenic:Hs3st2-Usp31 4:intergenic:F830001A07Rik-Rora 4:intergenic:Olfr458-Olfr457 4:intron:Foxp2 4:intergenic:AC133283.1-Gm23139 4:intergenic:Gm25335-Magi2 4:intergenic:Gm22538-Bche 4:intergenic:Gm24295-Mef2c 4:intergenic:Gm14832-Eda 4:intergenic:Gmds-Gm11380 Off-target primers show all... show less...
313 / rev	`TCTTAAAAGACCATAAAAAA CGG` `.................... ...` Low GC content Enzymes: HpyCH4III Cloning / PCR primers	42	56	45	46	61	69	0 - 0 - 22 - 75 - 658 0 - 0 - 3 - 2 - 14 755 off-targets	More than 500 off-targets, showing 16 with score >1.0 3:intergenic:Tmem45b-Barx2 3:intergenic:Sult2a5-Gm15436 3:intergenic:Fgf13-Gm14887 2:intergenic:Gm23591-Speer2 4:intergenic:Gm12895-Gm12877 4:intergenic:Gm12878-Gm12892 4:intergenic:Gm12896-Gm12897 4:intron:2210408I21Rik 4:intron:Rims2 4:intergenic:Vmn2r99-Vmn2r-ps121 4:intergenic:Gm5742-Gm22223 4:intergenic:Gm22331-Gm24937 4:intergenic:Ythdc2-Gm26928 2:intergenic:Cast-Hnrnpa1l2-ps 4:intergenic:Gm26128-Map2k4 2:intergenic:Acad9-D3Ertd254e Off-target primers show all... show less...
335 / fw	`GTTTTTTATGGTCTTTTAAG AGG` `.................... ...` Not with U6/U3 Enzymes: Tru1I Cloning / PCR primers	35	56	48	34	62	76	0 - 0 - 4 - 114 - 342 0 - 0 - 1 - 16 - 5 460 off-targets	4:intergenic:Gm13578-Grb14 4:intergenic:Cabp2-Cdk2ap2 4:intron:Vmn2r68 4:intergenic:Trps1-Eif3h 4:intergenic:Cetn1-Colec12 4:intergenic:Gm13572-Baz2b 4:intron:Vmn2r-ps66 4:intergenic:Gm11986-U7 4:intron:Psd4 3:intron:Acly 3:intergenic:Gm21918-Gm21832 3:intergenic:Gm21843-1700040F15Rik 3:intergenic:Gm21588-Gm21599 3:intergenic:Gm20794-Gm21918 3:intergenic:Gm21914-Gm21852 3:intergenic:Gm21801-Gm21914 3:intergenic:Gm21767-Gm20822 3:intergenic:Gm21758-Gm21843 3:intergenic:Gm21751-Gm21271 3:intergenic:AC201846.1-Gm20910 3:intergenic:Gm21529-Gm21539 3:intergenic:Gm21572-Gm21753 3:intergenic:Gm21170-Gm21751 3:intergenic:Gm21724-Gm21405 3:intergenic:Gm21805-Gm21241 3:intergenic:Gm20901-Gm21865 3:intergenic:Gm21861-Gm21758 3:intergenic:Gm20810-Gm21853 3:intergenic:Gm21599-Gm21810 3:intergenic:AC201846.1-Gm20910 3:intergenic:Gm21679-Gm21529 3:intergenic:Gm21247-Gm21256 3:intergenic:Gm21633-Gm21745 3:intergenic:Gm21745-Gm21184 3:intergenic:Gm21723-Gm21821 3:intergenic:Gm21247-Gm21256 3:intergenic:Gm21822-Gm21763 3:intergenic:Gm20810-Gm21853 3:intergenic:Gm21861-Gm21758 3:intergenic:Gm21247-Gm21256 3:intergenic:Gm21943-Gm21724 3:intergenic:Gm21562-Gm21572 3:intergenic:Gm21626-Gm21633 3:intergenic:Gm21805-Gm21241 3:intergenic:Gm21821-Gm21842 3:intergenic:Gm20901-Gm21865 3:intergenic:Gm21803-Gm20837 3:intergenic:Gm21861-Gm21758 3:intergenic:Gm21539-Gm21908 3:intergenic:Gm21679-Gm21529 3:intergenic:Gm20810-Gm21853 3:intergenic:Gm21745-Gm21184 3:intergenic:Gm21767-Gm20822 3:intergenic:Gm21822-Gm21763 3:intergenic:Gm20837-Gm21860 3:intergenic:Gm21779-Gm21247 4:intergenic:Gm15197-Lace1 4:intron:Sox30 4:intron:Srek1 4:intron:Adcy10 4:intron:Taf8 4:intergenic:Gm13799-Gm13800 4:intergenic:Gm21739-Gm21856 4:intron:Mettl8 4:intergenic:Gm7191-Gm23494 4:intron:1700112E06Rik 3:exon:Ulbp1 4:intron:Nckap5 3:intergenic:Gm26327-Gm6851 4:intergenic:Gm10327-Sult3a1 4:intergenic:Gm13218-Gata3 3:intergenic:Gm10619-St8sia2 3:exon:Lmnb1 4:intergenic:Gm8521-Gm20918 3:exon:Scrn3 3:intron:Mnd1 4:intergenic:Gm24901-Gm25792 4:intergenic:Gm27025-Gm25700 4:intergenic:March1-Gm15356 4:intergenic:Gm8521-Gm20918 4:intergenic:Gm24984-Zwint 3:intergenic:Large-Gm23255 4:intergenic:Gm23225-Gm24127 4:intron:Ccdc132 4:intergenic:Olfr837-Olfr843 4:intron:Slc6a13 3:intergenic:Gm10384-Zfp706 4:intergenic:Tyro3-Gm13998 4:intergenic:Gm22438-Gm22159 4:intergenic:mmu-mir-6354-Gm5265 4:intron:Sema5a 4:intron:Cyp2c55 4:intergenic:Gm11997-Vwc2 4:intron:Tmeff2 4:intergenic:Cadm2-Gm15828 4:intergenic:Ankrd33b-Ropn1l 4:intergenic:Gap-CAAA01118383.1 4:intron:Fam81a 4:intergenic:Hsd3b3-Hsd3b6 4:intergenic:Gsg1-Pbp2 4:intron:Unc79 3:intron:Abce1 4:intergenic:Slc7a15-Pum2 4:intron:Dcdc2a 3:intron:Ubxn11 4:intron:Apool 4:intron:Skint4 4:intergenic:Lrrtm4-Gm26264 4:intergenic:Klf5-Gm22984 4:intergenic:Gm25258-Hao1 4:intergenic:Gm15399-Gm9372 3:intergenic:Gm24968-Gm22797 4:intergenic:Crry-ps-AC161214.1 4:intron:Uvrag 4:intergenic:Lrrc8d-Rps15a-ps5 4:intergenic:Vmn2r-ps111-Vmn2r89 4:intergenic:4933415F23Rik-Gm24176 4:intergenic:Wfdc1-Atp2c2 4:intergenic:Pdcd1-Gm23389 3:intergenic:Nyap2-Gm5530 4:intergenic:Opcml-Gm15606 4:intron:Arhgap12 4:intergenic:Gm6238-Abcb7 4:intron:Ptpn6 4:intergenic:Rora-F830001A07Rik 4:intergenic:Gm4487-Gm24073 4:intron:Rims2 4:intergenic:Irx2-Irx4 4:intron:Park2 4:intergenic:Gm13941-Gm15130 4:intergenic:Gm23893-Gm10181 4:intergenic:Olfr346-Olfr347 4:intergenic:Gm5432-Lpin1 4:intron:St8sia4 4:intergenic:Nf1-Gm11198 3:intergenic:Tll1-Mir710 4:intergenic:Mark1-Rab3gap2 4:intron:Ckm 4:intergenic:B3galtl-Rxfp2 4:intergenic:Syce1l-Adamts18 4:intergenic:Gm26755-Ssfa2 4:intergenic:Gm6462-Gm25586 4:intergenic:Gm15054-Hibadh 4:intron:Gbp4 3:intergenic:Rpl23a-ps13-Gm14491 4:intergenic:Icosl-D10Jhu81e 4:intergenic:Sowahc-P4ha1 3:intergenic:Eef1g-Ahnak 4:intergenic:Hsd17b11-Nudt9 4:intron:Hjurp 4:intron:Ptprb 4:intergenic:Gm4868-Gm23151 4:intergenic:Gm15812-Phactr1 4:intergenic:2010107G23Rik-Tspan15 4:intron:Gmds 4:intergenic:Ndufb5-Usp13 4:intron:Cwf19l2 3:intergenic:Gm22269-Gm8177 3:intergenic:Cyp3a44-Cyp3a11 4:intergenic:Gm22132-Nr5a2 4:intergenic:Rbfox1-Gm23476 4:intergenic:Vmn2r89-Gm7247 4:intergenic:Gm26947-Mir466j 4:intergenic:Pcdh17-Gm23926 4:intergenic:Gm13227-Gm13230 4:intergenic:Gm13212-Gm26763 4:intron:Hs6st3 4:intron:Mcu 4:intron:Cep128 4:intergenic:Gm14768-Gm5940 4:intron:Clasp1 3:intergenic:Ets2-2810404F17Rik 4:intergenic:Sec16b-Brinp2 4:intergenic:Tyrp1-Lurap1l 4:intron:Cyp2j13 4:intergenic:Macf1-Gm12922 3:intergenic:Gm25985-Gm6404 4:intron:Ngef 4:intron:Rassf2 4:intergenic:Ptpdc1-Barx1 4:intergenic:Sec61b-NAMA_1 4:intergenic:Gm11845-Tmem64 4:intergenic:Gm5514-Trpm3 4:intergenic:Gm26913-Gm4076 4:intergenic:Cdh17-Gm22477 4:intergenic:Fgf10/Gm16263-Fgf10 4:intron:Agbl4 4:intergenic:Btbd9-Gm9874 4:intergenic:Gm22660-Laptm5 4:intergenic:Gm12034-Sertad2 3:intergenic:Rpl23a-ps1-Gm3852 2:intergenic:Gm23591-Speer2 4:intergenic:Gm12367-Gm12368 4:intergenic:Gm20865-Gm21065 4:intergenic:Gm21829-Gm20912 4:intergenic:Gm21802-Gm25729 4:intron:Hltf 4:intergenic:Gm9002-Kifc5c-ps 4:intergenic:Alg13-Trpc5 4:intron:Tmtc2 4:intergenic:Gm11986-U7 4:intergenic:Dcc-Gm25509 4:intergenic:Gm8584-Gm9637 4:intergenic:Gm25634-Hs6st1 4:intron:Bpi 4:intron:a 4:intergenic:Ccdc50-Fgf12 4:exon:Lrrc49 3:intergenic:Tspan3-C230081A13Rik 4:intergenic:Gm25700-Gm24935 3:exon:4930480K23Rik 4:intron:Gm8488 3:intron:Nlk 4:intergenic:Gm24188-Cdon 4:intron:Gen1 4:exon:Zfp830 3:intergenic:Plin2-Dennd4c 4:intergenic:Gm25480-Gm11220 4:intergenic:Gm7722-Gm14609 4:intergenic:Gm5083-Gm27007 4:intergenic:Sphkap-Gm25754 3:intergenic:8430427H17Rik-Commd7 3:intergenic:A630012P03Rik-Mir363 4:intron:Gbp9 4:intergenic:Gm15397-Gm6407 4:intron:Ddx60 4:intergenic:Gm14776-Il1rapl1 4:intergenic:Gm5620-Zic1 4:intron:Ints8 4:intergenic:Gm24910-Gm25784 4:intergenic:Nfil3-Ror2 4:intron:8430427H17Rik 4:intron:Pla2g4a 4:intergenic:Eya1-Msc 4:intergenic:Gm9864-Gm23439 4:intergenic:Gm11836-Slc26a7 4:intergenic:Gm24127-Pcdh9 4:intergenic:C330008G21Rik-Ptprt 3:intergenic:Gm14982-Gm26209 3:intergenic:Osmr-Lifr 4:intron:Wfdc8 4:intron:Msra 3:intergenic:Gm23345-Usp47 4:intergenic:Unc13c-n-R5s86 4:intron:Lcp2 3:intron:Opcml 4:intergenic:Zfp58-Zfp87 3:intron:Polq 4:intergenic:Gm14751-Rps24-ps3 4:intergenic:Mir690-Mb21d2 4:intergenic:Igbp1b-Gm25434 4:intron:Wdr26 4:intergenic:Pvt1-Gm24810 4:intergenic:Gm11835-Gm11814 4:intergenic:Gm9578-Gm22891 4:intergenic:Olfr773-Olfr774 4:intergenic:Ncald-Gm15942 4:intergenic:Gm22434-Prrx1 3:intergenic:Gm20424-Lbx1 4:intergenic:Tram1l1-Gm4617 4:intergenic:Rapgef4-Gm13634 4:intergenic:Mir690-Mb21d2 4:intron:Zfp407 4:intron:Chek2 4:intergenic:Mki67-Gm9341 4:intergenic:Vmn1r211-Vmn1r-ps116 3:intergenic:Gm12292-Elac2 4:intergenic:Cntnap5a-Gm19965 4:intergenic:Ccdc71l-Nampt 4:exon:Cntn4 3:intron:Dmd 3:intergenic:Scgb1b3-Scgb2b7 3:intergenic:D10Wsu102e-Aldh1l2 4:intron:Ccdc146 4:intergenic:Egfem1-Mir551b 4:intron:Dennd1a 4:intron:Cdca7l 4:intergenic:Gm11260-Gm11261 4:exon:3110057O12Rik 4:intron:Nol9 4:intergenic:Gm22108-Gm21370 4:intergenic:Gm21188-Gm24798 4:intergenic:Gm26405-Gm9274 4:intergenic:Gm20822-Gm21809 4:intergenic:Gm20910-Gm20909 4:intergenic:Gm20828-Gm20812 4:intergenic:Slc1a3-Gm5210 4:intergenic:Igsf11-Gm24795 4:intron:Thsd7b 4:intron:Popdc3 4:intergenic:Gm14241-Gm11449 4:intron:Oca2 4:intergenic:Igkv4-78-Igkv4-74 4:intergenic:Lrrfip1-Rbm44 4:intergenic:Olfr1137-Olfr1138 4:intergenic:Rnase10-Rnase9 4:intron:Gm11867 4:intergenic:Igf2bp3-Tra2a 4:intergenic:Plod2-Gm9621 4:intergenic:AA414768-Gm14534 4:intergenic:Olfr747-Olfr748 4:intron:Mipol1 4:intergenic:Gm7931-Slit2 4:intergenic:Zbtb20-Gm15711 3:intergenic:Gm9149-Gm24766 3:intergenic:Scgb2b15-Gm25817 3:intergenic:G630030J09Rik/Gramd1a-Scgb1b2 3:intergenic:Scgb1b3-Scgb2b7 3:intergenic:Scgb2b15-Gm25817 3:intergenic:Gm5329-Gm9135 3:intergenic:Scgb1b21-Gm12770 3:intergenic:Scgb1b3-Scgb2b7 3:intergenic:Scgb1b7-Gm5329 3:intergenic:Scgb2b17-Gm9162 4:intergenic:Fscb-Gm24066 4:intergenic:Pisd-ps2-Scaf8 4:intron:Zcchc24 4:intergenic:Gm14616-Gm14444 4:intron:Pah 4:intergenic:Srd5a2-Memo1 4:intergenic:Vmn2r102-Vmn2r-ps122 4:intergenic:Wdpcp-Rpl15-ps1 4:intergenic:Gm23729-Cdkal1 4:intergenic:Gm14666-Gm14821 4:intergenic:Prkg2-Rasgef1b 4:intergenic:AY512915-Suclg2 4:exon:Atrx 3:intergenic:1700109G15Rik-Gm11431 4:intergenic:Vmn2r-ps111-Vmn2r88 4:intergenic:Rarb-Thrb 4:intergenic:Gm25495-Gm23225 4:intron:Acoxl 4:intron:Atp8b1 4:intergenic:Rpl7a-ps5-Cntnap5c 4:intron:Scamp1 4:intron:Serpinb13 4:intron:Lhfp 4:intron:Hk1 4:intergenic:Ncam2-Gm23355 4:intergenic:RP24-196H4.1/Gm4070-Gm4070 4:intergenic:Zc2hc1a-Il7 4:intergenic:Gria4-Gm24896 4:intergenic:Hey2-Gm24168 4:intergenic:Rock1-Greb1l 4:intergenic:RP23-142E18.4-Gvin1 4:intergenic:Setmar-Sumf1 4:intron:Clec2g 4:intergenic:Vmn1r219-Vmn1r220 4:intron:Ptprd 4:intron:Rpgrip1l 4:intergenic:A530021J07Rik-Gm10610 4:intergenic:Gm26137-Gm23733 4:intergenic:Pcdh9-Gm23324 4:intron:Abi3bp 4:intron:Prps2 4:intron:Tusc3 4:intron:C230014O12Rik 4:intergenic:Gm17641/1110032F04Rik-Ift80 3:intron:Spock1 4:intergenic:Slc23a1-Mzb1 4:intergenic:Vmn2r96-Vmn2r-ps115 4:intergenic:A630010A05Rik-Snai2 4:intron:Kansl1 4:intergenic:Armc3-4930447M23Rik 4:intergenic:Gm26263-Pax3 4:intron:Asrgl1 4:intron:Lsamp 4:intergenic:Fgf12-Mir690 4:intron:Kat2b 4:intergenic:Gm25396-Slc25a46 4:intron:Dpp6 3:intergenic:Gm20837-Gm21860 3:intergenic:Erdr1-Gm21748 3:intergenic:Erdr1-Gm21748 3:exon:Gnb4 4:intron:1500012K07Rik 4:intergenic:Brinp3-Gm9931 4:intergenic:Gm22163-Robo2 4:intergenic:Gm25437-Gm22684 4:intron:Tgfbr1 4:intron:Dcaf8 3:intron:Hormad2 4:intergenic:Gm24948-Nova1 4:intergenic:9130204L05Rik-Gm23697 3:intergenic:Gm17215-DAOA-AS1_1 4:intron:Eif4e 3:intron:Cyld 4:intergenic:Col22a1-Gm23217 3:intergenic:Agpat4-Map3k4 4:intergenic:Gm12878-Gm12892 4:intergenic:Gm24694-Gm25863 4:intergenic:Gm15802-Igsf11 4:intergenic:Gm11835-Gm11814 4:intergenic:Pi15-Gm16070 4:intergenic:Psg29-Gm24520 4:intergenic:RP24-194K24.6-RP24-194K24.7 4:intergenic:Gm22848-Rp1 4:intergenic:Gm25825-Gm5648 4:intergenic:Gm23903-Ank 4:intergenic:Ighv13-1-Ighv3-6 4:intergenic:Chi3l4-BC051070 4:intergenic:6330444E15Rik-n-R5s153 4:intergenic:Steap1-Gm26825/Gm15459 4:intergenic:RP24-194K24.9-RP24-84K14.10 4:intergenic:Gm12893-Gm12895 4:intergenic:Gm5611-Chordc1 4:intergenic:Gm5852-Gm5773 4:intergenic:Gm24850-Gm25387 4:intergenic:Gm10480-5730507C01Rik 4:intergenic:Gm12659-Cyp2j8 4:intron:Abcc4 4:intergenic:Psg29-Gm24520 4:intergenic:Slco3a1-Gm22326 4:intergenic:Ocln-Marveld2 4:intron:Csgalnact2 4:intergenic:Gm23665-Csmd3 4:intron:Grm1 4:intergenic:Eva1c-Gm7831 3:intron:Kcnmb2 2:intergenic:Gm23815-Gm7627 3:intergenic:Tgif2lx1-Gm7485 2:intergenic:Ucn3-Gm22460 3:intergenic:Gm16418-Gm4846 4:intergenic:Ubr5-Odf1 4:intergenic:Dsg2-Gm16090/Ttr 4:intergenic:Gm26211-Mboat2 3:intergenic:Fam43a-Gm26295 4:intergenic:Qpct-Cdc42ep3 3:intergenic:Gm13345-4921504E06Rik 3:intergenic:Gm15975-Gm23896 4:intergenic:S100a9-Pglyrp4 4:intergenic:Gm22312-Magi1 3:intron:Htr1f 3:intergenic:Zfp747-9130019O22Rik 4:intron:Bbs9 4:intergenic:Trps1-Eif3h 4:intergenic:Itm2b-Med4 4:intergenic:Gm15402-Tcf7l1 3:intergenic:Gm23607-Gm17094 4:intergenic:Gm20713-Gpc5 4:intergenic:Bmper-Npsr1 4:intergenic:Trps1-Eif3h 4:intergenic:Gm25665-Gm24469 4:intron:Prdm15 4:intergenic:Cyp3a57-Cyp3a16 3:intron:Ascc1 2:intron:Fxr1 4:intergenic:Sfrp1-Zmat4 4:intergenic:Gm23011-Gm26691 4:intergenic:Ercc6l2-Hsd17b3 4:intergenic:Gm11846-n-R5s182 4:intergenic:Eftud1-4933406J10Rik 4:intergenic:Gm9381-Ndst4 4:intron:Invs 3:intron:Kat6b 4:intergenic:Gm14379-2010204K13Rik 4:intergenic:Has2os/Has2-Has2os 4:intergenic:Fam43a-Gm26295 4:intron:Sipa1l1 4:intergenic:Dhfr-C030017D09Rik Off-target primers show all... show less...
43 / rev	`TTTATAAATGAGACAAAGGA AGG` `.................... ...` Cloning / PCR primers	23	51	65	36	77	80	0 - 1 - 21 - 101 - 809 0 - 0 - 2 - 18 - 103 932 off-targets	More than 500 off-targets, showing 28 with score >1.0 3:intron:Stard8 2:intergenic:Gm9800-Fhit 2:intergenic:Gm23763-Gm25609 3:intergenic:Gm12725-Gm12724 3:intron:Gpc6 4:intergenic:Gm8805-Crygf 2:intergenic:Gm25248-Gm26326 3:intergenic:Cav2-Cav1 1:intergenic:Slc16a7-Lrig3 3:intergenic:AC133283.1-Gm23139 3:intergenic:Lum-Kera 3:intergenic:Gm5272-1700008P02Rik 3:intergenic:Gm7820-Gm7821 2:intergenic:Fam19a4-Eogt 2:intergenic:AC112986.1-Tsn 2:intergenic:AC112986.1-Tsn 2:intergenic:AC112986.1-Tsn 2:intergenic:AC112986.1-Tsn 3:intergenic:Card11-Gm26113 3:intergenic:Gm24635-Arrdc3 3:intergenic:Rasal2-Gm6058 3:intergenic:Gm5506-Gm5839 3:intergenic:Heca-Abracl/Gm17711 3:intergenic:Cdh6-Gm9630 3:intergenic:Gm26345-Gm10566 4:intergenic:Gm12004-4930554G24Rik 3:intergenic:Hs3st2-Usp31 2:intron:Cdh10 Off-target primers show all... show less...
256 / fw	`ATTCTGTTTTGTTTGCTTTG AGG` `.................... ...` Not with U6/U3 Inefficient Cloning / PCR primers	16	30	38	18	75	84	0 - 3 - 54 - 258 - 1489 0 - 1 - 8 - 37 - 29 1804 off-targets	More than 500 off-targets, showing 30 with score >2.0 1:intergenic:Mrs2-Dcdc2a 2:intergenic:St3gal1-Zfat 2:intergenic:Mybpc1-Spic 2:intron:Tbc1d9b 2:intergenic:Gm25062-Gm15487 3:intron:Rlf 3:intergenic:Gm7493-Sntg1 2:exon:Shroom3 3:intergenic:Rps12l2-D8Ertd82e 3:intergenic:Rpl36-ps1-Sphk1 3:intron:Cdyl 3:intergenic:Sftpd-Gm7925 3:intergenic:Gm7995-Gm17164 3:intergenic:Tdrd3-Rps3a2 3:intergenic:1700024B05Rik-Gm17063 3:intergenic:Gm10378-Gm17162 2:intergenic:Plxdc2-Gm24933 2:intergenic:Pdzrn4-Gm4335 2:intergenic:Epcam-Msh2 2:intergenic:Rpl13a-ps1-Sorcs1 3:intergenic:Smlr1-Tmem200a 3:exon:Krt222 2:intron:Tsc22d4 2:intergenic:D030055H07Rik-Wdr82 2:intergenic:Gm5973-Gm9915 2:intron:Frmd4a 1:intron:Zeb2 2:intron:Kcnc2 1:intron:Dock11 2:intergenic:Tmem126b-Dlg2 Off-target primers show all... show less...
234 / rev	`TCAAAGCAAACAAAACAGAA TGG` `.................... ...`	0	0	64	20	67	87	Repeat

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