CRISPOR

Mus musculus (mm10), chr7:42263353-42264152, forward genomic strand

Your input sequence is 800 bp long. It contains 57 possible guide sequences.
Shown below are their PAM sites and the expected cleavage position located -3bp 5' of the PAM site.
Click on a match for the PAM NGG below to show its 20 bp-long guide sequence. (Need help? Look at the CRISPOR manual)
Colors green, yellow and red indicate high, medium and low specificity of the PAM's guide sequence in the genome.

Position  0         10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480       490       500       510       520       530       540       550       560       570       580       590       600       610       620       630       640       650       660       670       680       690       700       710       720       730       740       750       760       770       780       790       
Variants  ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................
Sequence  gtgaactgaaaacattgttcccttgagtttatccacagccccttgtctttaaaatcttcccacttcttttctgccatgattgctcaggattgttgggaagggtatgatatctaacccacatttatggcttagcaatacaaagtctgtcagtgttttatgtattatttgtcatttactataaaaagaatctatgaagaagacttatgtttgcactaatctgtgggtacatcaataagtcattactggttgactttgtactttagcacaataagggtattaagttatccctgtggatctatcacttaccttaccacagagtcttgccattttcatagtgaaatgcatgttttctattttatggagtgaatcttatattacatcagataaagattggatccatatgtaacatatgtgcccactttagtaggagatgacatgtcagataaacacagtcattgtcatatccttcatggtttacaggttgatgagaaagatgattcttttattttactgggaagtatgcatagcatcttacagcactatgaaacctagccaggagaaatatggtagattatttactgcatgattactccatattctgtggtcaagtatgttgtgccctcaacaatggagtctttccatcaagtccaacaggattcaaattgtgtcttcctgatatatattctagataaagagtatgaaagatgtatgatatttgactttcttaatgtgattcagaattgttattctaattgtatggaacttcattgggatgtacagatttatggtatggttaattt
                             CCC---       CCA---  CCT---            CCC---         CCA---   −−−AGG  −−−TGG                  CCC---                                                                                                 −−−TGG                 −−−TGG                     −−−AGG            CCC---              CCT---            CCA---                          −−−TGG                           −−−TGG  CCA---            CCC---  −−−AGG                                    CCT---     −−−AGG                           −−−TGG                                 CCT---        −−−TGG                        CCA---                     CCC---              CCA---   CCA---                  CCT---                                                                             −−−TGG     −−−TGG           −−−TGG
                              CCT---            CCC---               CCA---                          −−−GGG                  CCA---                                                                                                 −−−GGG                                            −−−GGG            CCT---                  CCA---                                                                                                   CCA---                                              −−−TGG                                    −−−GGG                                     CCA---                                        −−−TGG               CCT---                        −−−AGG                                                                                                               −−−GGG               −−−TGG
                                                 CCC---                                                  −−−AGG                    −−−TGG                                                                                                                                                                −−−TGG                                                                                                                                                                                                                                                                  −−−AGG                                                                    −−−TGG
                                                                                                          −−−GGG

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Predicted guide sequences for PAMs

Ranked by default from highest to lowest specificity score (Hsu et al., Nat Biot 2013). Click on a column title to rank by a score.
If you use this website, please cite our paper in NAR 2018. Too much information? Look at the CRISPOR manual.

Download as Excel tables: Guides / Guides, all scores / Off-targets / Saturating mutagenesis assistant

Position/ Strand	Guide Sequence + PAM + Restriction Enzymes + Variants Only G- Only GG- Only A-	MIT Specificity Score	CFD Spec. score $The CFD specificity score, inspired like guidescan.com, behaves like the MIT specificity score, but it is based on the more accurate CFD off-target model, from <a href='http://www.nature.com/nbt/journal/v34/n2/full/nbt.3437.html'>Doench 2016</a>, which is also used by Crispor to rank the off-targets. The CFD specificity score correlates better than the MIT score with the total off-target cleavage fraction of a guide, see <a target=_blank href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731277/'>Tycko et al, Nat Comm 2019</a> and also the <a target=_blank href='/manual/#faq'>CRISPOR manual</a>.$	Predicted Efficiency Show all scores		Outcome		Off-targets for 0-1-2-3-4 mismatches + next to PAM	Genome Browser links to matches sorted by CFD off-target score exons only chr7 only
				Doench '16	Mor.-Mateos	Out-of-Frame	Lindel
415 / rev	`ATGTCATCTCCTACTAAAGT GGG` `.................... ...` Enzymes: Bsp1286I, BaeGI Cloning / PCR primers	86	94	54	50	55	77	0 - 0 - 2 - 9 - 80 0 - 0 - 0 - 1 - 0 91 off-targets	4:intron:Gria1 4:intergenic:Gm14832-Eda 4:intergenic:Gm23083-Gm24773 4:intron:Sh3gl2 4:intergenic:Gulp1-Col3a1 4:intergenic:U6-A430048G15Rik 3:intergenic:Serp2-Lacc1 4:intergenic:Rragb-Gm15153 4:intron:Tsen2 4:intergenic:Gm12073-Gm12074 4:intergenic:Gm25295-Gm24546 4:intergenic:Gm27039-Adcyap1 4:intergenic:PVT1_3-Ndufa4 4:intergenic:Gm13463-Lrp1b 4:intron:Rnf213 3:intron:Tmem2 4:intron:Ccser1 4:intron:Zfml 3:intergenic:Ankrd7-Gm22718 4:intergenic:Gm13579-Gm13581 4:intergenic:Gata4-Blk 4:intergenic:Gm20516-Gm21947 4:intergenic:ACEA_U3-U3 4:intergenic:RP23-131O4.2-Gm23470 4:intergenic:Sept7-Eepd1 4:intron:Chp1 4:intergenic:Gm23339-Gm25570 4:intergenic:Gm1815-Zfp353-ps 4:intergenic:Gm18294-Vmn2r3 4:intergenic:Kcnf1-Pdia6 4:intergenic:Tbc1d32-Rps8-ps1 4:intergenic:Gm23794-Nhs 3:intron:D430042O09Rik 4:intergenic:Gm23485-Rpl34-ps1 4:intergenic:Gm5839-Gm25440 4:intergenic:A830092H15Rik-Gm25773 4:intron:Ptprk 4:intron:Bmpr1b 3:intron:Gls2 4:intergenic:AC158354.1-Gm4804 4:intron:Fgf13 4:intron:Gm17359 3:intergenic:Cdh10-Gm25976 4:intron:Mast2 4:intron:Usp34 4:intron:Grik2 4:intergenic:Eda2r-Gm9062 2:intergenic:Gm25423-Gm26054 4:intergenic:Gm23015-4932441J04Rik 4:intergenic:Aoah-Olfr1370 4:intergenic:Snx30-Gm12543/Snx30 4:exon:Msx2 3:intron:Ddx21 4:intergenic:Gm24720-Tshz1 4:exon:Sis 4:intron:Bphl 4:intergenic:Gm16035-Gm24131 4:intergenic:Gm23960-Gm26373 4:intergenic:Bmper-Npsr1 4:intron:E130308A19Rik 4:intergenic:Gm26638-Gm26807/Tmem178b 4:intergenic:Ahctf1/Gm10518-Cdc42bpa 4:intergenic:Oca2-Gabrg3 3:intron:Gm2824 4:intergenic:Gm25659-Gm25644 4:intergenic:Gm24987-Socs6 4:intergenic:Rhox13-Gm14556 4:intergenic:4931417E11Rik-Gm26179 4:intron:Dach1 4:intron:Dym 4:intergenic:Gm24863-Gm24635 2:intron:Stat5b 3:intergenic:4930415H17Rik-Gm14190 4:intergenic:Gm23821-Tgfb2 4:intergenic:Akr1c12-Akr1c6 4:intron:Psen2 4:intergenic:A830012C17Rik-Gm11780 4:intron:Asap1 4:intergenic:Rpl10l-Mdga2/MDGA2 4:intergenic:Chrnb3-Chrna6 4:intron:Unc5c 4:intergenic:Gm9312-Gm16372 4:intron:Kcnd3 4:intergenic:Gm2546-A230065N10Rik/Me3 4:intron:Ido2 4:intergenic:Neil3-Vegfc 4:intergenic:Gm25258-Hao1 4:intron:Zzef1 4:intron:Piezo2 4:intron:Mroh9 4:intergenic:Tpbg-Ube2cbp Off-target primers show all... show less...
416 / rev	`CATGTCATCTCCTACTAAAG TGG` `.................... ...` Enzymes: Bsp1286I, BaeGI Cloning / PCR primers	86	91	59	33	56	82	0 - 0 - 2 - 8 - 76 0 - 0 - 0 - 0 - 1 86 off-targets	3:intron:Exoc6b 4:intron:Rad51b 4:intergenic:Trim29-Pvrl1 4:intron:Ndc1 4:intron:Gria1 4:intron:Gm101 4:intron:Pstpip2 4:intergenic:n-R5s165-Lmcd1 4:intergenic:Gm13498-Rnd3 4:intergenic:Eri1-Gm16793 4:intergenic:Dennd4b-Gatad2b 4:intergenic:Fam184a-Man1a 4:intron:Lhfpl1 4:intergenic:Tle3-Rplp1 4:intergenic:Gm13255-Gm13217 4:intron:Arhgap10 4:intergenic:Gm26373-Kcnd2 4:intron:Tsen2 4:intergenic:Olfr859-Olfr860 4:intergenic:Olfr857-Olfr58 4:intron:Lingo2 3:intergenic:Snx30-Gm12543/Snx30 4:intron:Fmnl3 4:intergenic:Krtap8-1-Krtap7-1 4:intergenic:Gm21614-Gm23249 4:intergenic:Krtap20-2-Krtap21-1 4:intergenic:Fam184b-9630001P10Rik/Fam184b 4:intergenic:Gm23339-Gm25570 4:intergenic:Leprel1-Cldn1 4:intergenic:Chn2-9530036M11Rik 4:intergenic:Gm23965-Gm23537 4:intergenic:Gm24691-Grm8 4:intergenic:Gm24987-Socs6 4:intergenic:Gm25778-Ppapdc1a 4:intergenic:Id2-Gm22527 2:intergenic:Gm24686-Zfp804b 4:intron:Cdh8 4:exon:Kcnt2 4:intron:Prlr 4:intron:Ncor2 4:intergenic:Gm22211-Gm6181 3:intergenic:Gm24402-Gm10138/Ivns1abp 3:intron:Psen2 4:intron:Dock9 4:intergenic:Ccdc37-Klf15 4:intergenic:Gm23727-Gm20594 3:intergenic:Gm9002-Kifc5c-ps 4:exon:Ttc21a 4:intergenic:Gm1815-Zfp353-ps 4:intergenic:Dmrta1-Gm12629 4:intergenic:Gm20516-Gm21947 4:intergenic:mmu-mir-6348-mmu-mir-6354 4:intergenic:Olfr181-Olfr183 4:intergenic:Cpa4-Cpa5 4:intron:Mroh9 4:intron:Gabrb1 4:intron:Gm15514 4:intergenic:Aldh1a2-Polr2m/Gcom1 4:intergenic:Gm23965-Gm23537 4:intergenic:Gm25434-Rergl 4:intergenic:Gm22484-Gm23555 3:intron:Fancg 4:intergenic:Gm22950-Slamf1 4:intron:Tcf7l2 4:intergenic:Tmem126b-Dlg2 4:intron:Kif21b 4:intergenic:9330185C12Rik-Cntnap5a 3:intron:Nptn 4:intron:Dpyd 4:intron:Unc5c 4:intergenic:Hba-ps4-Neurl1b 4:intron:Cyp2j5 3:intergenic:Mesdc2-9930013L23Rik 4:intergenic:Spag16-Gm23422 4:intergenic:Grin3a-Gm12458 4:intergenic:Cmtm7-Gm9888 2:intron:Camta1 4:intergenic:Gm8213-Fam21 4:intron:Amph 4:intergenic:Gm12469-Gm25687 4:intergenic:Gm22508-Ccdc68 4:intron:Lhfpl2 4:intron:Focad 4:intron:Tmem117 4:intergenic:Ccser2-Rgr 4:intron:Alk Off-target primers show all... show less...
287 / rev	`AAGGTAAGTGATAGATCCAC AGG` `.................... ...` Enzymes: MflI Cloning / PCR primers	84	90	54	76	78	79	0 - 0 - 1 - 7 - 95 0 - 0 - 0 - 1 - 0 103 off-targets	3:intergenic:Id2-Gm22527 4:intergenic:Zfp330-Rnf150 4:intron:Asph 4:exon:Slc5a12 4:intergenic:Gm7580-Slco3a1 4:intron:Stac 4:intergenic:Dnhd1-Mrps36-ps2 4:intron:Arhgap25 4:intergenic:Hs6st1-Uggt1 4:intergenic:Nptx2-Gm6272 4:intergenic:Ppp1r2-ps7-Gm26131 4:intron:Alcam 4:intergenic:Oxct1-Plcxd3 4:exon:Trpm5 4:intergenic:Gm14804-Gm6973 4:intron:Tec 3:intergenic:E130006D01Rik-Miat/MIAT_exon5_3/MIAT_exon5_2/Gm26953/MIAT_exon5_1 4:intron:Ttc6 4:intergenic:Kat6a-Ank1 4:intergenic:Gm23324-Gm24680 4:intron:Plat 4:intergenic:Gm9959-Gm9386 4:intergenic:Gm6369-Armc1 4:intergenic:Gm13750-Gm25748 4:intron:Kazn 4:intron:Tns1 4:intergenic:Gm9621-Gm24200 4:intron:Hus1 4:intergenic:Gm25724-Gm24280 4:intron:Slmo2 4:intergenic:Gm23178-Gm6878 4:intergenic:Irx5-Irx6 4:intergenic:Gm5593-Vmn2r60 4:intron:Vcpip1 4:intergenic:Hnrnpa1l2-ps-Pcsk1 3:exon:Lrrc37a 4:intergenic:Gm8183-Tmem135 4:intergenic:Gm10155-Lat 4:intron:Pir 4:intergenic:Gm23609-Gcm1 4:intergenic:Cntn6-Gm23339 4:intergenic:Gm7931-Slit2 4:intergenic:Gm23986-Gm24929 4:intron:Grhl1 4:intergenic:Tmem200c-Epb4.1l3 4:intergenic:Snx16-Gm22795 4:intergenic:Gm20581-Gm20579 3:intron:Gm11992 3:intron:Auts2 4:intergenic:Gm22934-A530021J07Rik 4:intergenic:Gm26963-Ypel5 4:intron:Npas3 4:intron:Prrc2c 4:intron:Zfp518a 4:intergenic:Gm22798-Cog6 4:intergenic:Tmem132e-Gm24612 3:intergenic:Tspan9-Gm26338 4:intron:Tmem45b 3:intergenic:Gm25985-Gm6404 4:intron:Stk32c 4:intergenic:Gm25127-Gm9912 4:intergenic:Gm15802-Igsf11 4:intergenic:Gm3848-Ptprg 4:intergenic:Kctd1-Aqp4 4:intergenic:Dopey2-Morc3 4:intron:Ccdc171 4:intergenic:Gm13321-Pter 4:intron:Asic2 4:intron:Shcbp1 4:intergenic:Gm6954-Gm14733 4:intergenic:Gm6954-Gm14733 4:intergenic:Tspan13-Gm5434 4:intergenic:Npdc1-Fut7 4:intergenic:Sema5a-Sdc2 4:intergenic:Gm22120-Vrk1 4:intergenic:Samd13-Prkacb 4:intergenic:Plb1-Gm5864 4:intergenic:Gm26181-Triml1 4:exon:Cdhr1 4:intron:Abi3bp 4:intergenic:Eda-Gm14832 4:intergenic:Gm13578-Grb14 4:intergenic:Fam19a5-mmu-mir-6393 4:intergenic:Gm16578-Cplx2 4:intergenic:Scd3-Scd2 4:intergenic:Olfr24-Olfr828 4:intergenic:Agtr1a-Gm11369 4:intergenic:Gm9884-Gm24064 4:intergenic:Ldoc1l-Prr5 4:intron:Grm4 4:intergenic:Gh-Cd79b 4:intron:Ocrl 4:intergenic:Prickle1-Adamts20 4:intergenic:Pik3r1-Gm25808 4:intergenic:Tacc1-Gm10689 2:intergenic:Dlx2-Gm1631 4:intergenic:Gja10-Gm11933 4:intron:Med13 4:intergenic:ZNRD1-AS1_2-Nsmce4a 4:intergenic:Slc24a3-Gm22390 4:exon:Hmcn1 4:intron:Rere 4:intergenic:Gm14619-Sh2d1a Off-target primers show all... show less...
426 / fw	`ACATATGTGCCCACTTTAGT AGG` `.................... ...` Cloning / PCR primers	80	89	44	26	58	77	0 - 0 - 0 - 18 - 96 0 - 0 - 0 - 3 - 1 114 off-targets	3:intron:Tmem135 4:intergenic:Gm22848-Rp1 4:intergenic:Gm26031-Gm9801 4:intergenic:Gnas/Gm20721-Nelfcd 4:intergenic:Gm23891-1700018B24Rik 4:intron:Dock4 4:intergenic:Cnksr3-Ulbp1 4:intergenic:Gm6768-Macc1 4:intergenic:Gm14984-Diap2 4:intron:Epha6 4:intergenic:Fgf9-Gm25614 4:intergenic:Gm14847-4930519F16Rik 3:intron:Onecut2 4:intergenic:Wnk1-Ninj2 4:intergenic:Gm26580/Kcnq5-Rims1 4:intron:Gm12657 4:intergenic:Rbpsuh-rs3-Cntnap2 4:intergenic:Gm9801-Ndn 4:intergenic:4631405K08Rik-Plxna2 4:intergenic:Gm23937-Gm22944 4:intergenic:Gm24984-Zwint 4:intergenic:n-R5s209-Gm7846 4:intergenic:Eif3s6-ps1-Gm11995 4:intron:Ksr1 4:intergenic:Lingo1-Odf3l1 4:intron:Megf10 4:intergenic:Agtr1b-Gm7488 3:intron:C330002G04Rik 4:intron:Spock1 4:intergenic:Erc2-Wnt5a 4:intron:Bre 3:intergenic:Rpl7a-ps13-4933436I01Rik 4:intergenic:Vmn2r-ps58-Gm5594 4:intergenic:Klf13-Gm20670 3:intergenic:Gm12419-Cntln 3:intergenic:Zfyve28-Gm15513 4:intron:Tmc5 4:intergenic:Gm12587-Gm12591 4:intergenic:Slco3a1-Gm7580 4:intergenic:Gm23891-1700018B24Rik 4:intergenic:Ly86-Rreb1 4:exon:Gm16379/Gm16302 4:intergenic:Diras2-Syk 4:intergenic:Gm23591-Speer2 3:intron:Gabrp 4:intergenic:Pex5l-AC113989.1 3:intergenic:Gm24774-Pcdh17 4:intergenic:Wnt4-Cdc42 4:intergenic:Gm23437-Gm24818 4:intergenic:Gm26354-Gm7536 4:intron:Atad2 4:intergenic:Mir3072/Mirg-Dio3os 4:intergenic:Gm11820-Gm26250 4:intergenic:Jazf1-Gm26215 4:intron:Adam5 4:intron:Cnbd1 4:intron:9130011E15Rik 4:intergenic:Acot11-Gm12746 4:intron:Ccser1 4:intergenic:Rgs4-Gm22114 3:exon:Megf11 4:intron:Nckap5 4:intergenic:Hif1an-Gm26644 4:intron:Trpv2 4:intergenic:Gm24880-Gm10172 4:intergenic:Rims1-4933415F23Rik 4:exon:Ip6k1 4:intergenic:Vip-Fbxo5 4:intergenic:Cul2-G430049J08Rik 3:intergenic:Gm23400-Gm6594 4:intergenic:Prkg2-Rasgef1b 4:intergenic:Gm7488-Tbl1xr1 4:intergenic:Gm23178-Gm6878 4:intergenic:Ksr1-Gm11200 4:intron:Gm10373 4:intergenic:Gm26405-Gm9274 3:intergenic:4930412B13Rik-Gm13986 4:intron:Unc79 4:intergenic:Stab2-Gm24093 4:intron:Thap7 3:intron:Zfand6 4:intron:Spats2l 4:intergenic:Gm23558-Galc 4:intergenic:Mir876-Mir873 4:intron:Dnah12 4:intron:Sdccag8 4:intergenic:Gm25665-Gm24469 3:intron:Tbl1xr1 3:intron:Cers6 3:intergenic:Gpr88-Cdc14a 4:intron:Utp20 4:intergenic:Camkmt-Rpl31-ps16 4:intron:Dpp10 3:intron:Macf1 4:intron:Myo18a 4:intergenic:Gm15323-Ankrd55 4:intergenic:Wasf1-Gpr6 4:intergenic:Gm15637-Myl2 3:intergenic:mmu-mir-6899-Gm13748/Klf7 4:intergenic:Tex29-A230072I06Rik 4:intergenic:Gm4960-Gm8802 4:intergenic:Dab1-Gm12719 4:exon:Ids 4:intergenic:Gm22628-5033411D12Rik 3:intergenic:6330403L08Rik/Pdgfa-Prkar1b 4:intergenic:Tmem108-Nphp3 4:intron:AU019990 4:intergenic:Rgs17-Gm10945 4:intergenic:Epc1-4921524L21Rik 4:intron:Pmfbp1 4:intergenic:BC049762-Clk4 4:intron:Smad3 4:intergenic:Gm22183-mmu-mir-378d 4:intergenic:Gm26480-Gm6863 Off-target primers show all... show less...
791 / fw	`GGATGTACAGATTTATGGTA TGG` `.................... ...` Enzymes: Tru1I Cloning / PCR primers	79	86	44	40	64	82	0 - 0 - 1 - 14 - 116 0 - 0 - 0 - 2 - 1 131 off-targets	4:exon:Mdn1 4:intron:Mmd2 4:intergenic:Gm9992-Gm25872 4:intergenic:Gm26500-Tcp10c 4:intergenic:Rps6ka2-Tcp10a 4:intergenic:Sod2-Gpr31b 3:intron:Ddr2 4:intergenic:Gm20594-Gm4409 4:intergenic:Gm8730-Cdh5 4:intergenic:Spata18-Usp46 3:intergenic:Tmem117-Nell2 4:intron:2610307P16Rik 4:intergenic:Asns-C1galt1 4:intron:Kcnn2 3:intron:Dis3l2 4:intergenic:Gm13935-Gm10794 4:intergenic:Lelp1-Sprr2a1/Sprr2a2 4:intergenic:n-R5s10-Il1rapl1 4:intergenic:Gpx7-Gm12738 4:intergenic:Gm9308-Itgbl1 4:intron:Cd96 4:intergenic:Gm26812-Slc7a5 4:intron:Hs2st1 4:intergenic:Gm11851-Mmp16 4:intron:Sh3bp4 4:intergenic:Gm26354-Gm7536 4:intergenic:Gm25096-Gm25634 4:intergenic:Gm24443-Gm22529 4:intergenic:Mixl1-Acbd3 4:intergenic:March10-Tanc2 2:intergenic:Tmem65-Ube2d4 4:intergenic:Gm22566-Akap7 4:exon:Gm4262 4:intron:Mme 4:intergenic:Cdh9-Gm22032 4:intron:Hs3st5 4:intron:Arhgap15 4:intergenic:Gm23644-Gm15442 3:intergenic:Kcnip4-Gm19932 4:exon:Cln8 4:intron:Dnajc3 4:intergenic:Tpd52-Zbtb10 4:intergenic:Gm23154-Gm12604 4:intergenic:Gm25248-Gm26326 3:intergenic:Gm6614-Slco1a6 4:intron:Dcc 4:intergenic:Atp11a/Gm15347-Mcf2l 4:intergenic:1700015E13Rik-Nos1ap 4:intergenic:Gm22753-Usp34 4:intron:Stk38l 4:intergenic:Rgs21-Gm23672 4:intron:Rph3al 4:intergenic:A830092H15Rik-Gm25773 4:intron:Skint4 4:intron:Bckdhb 3:intron:Lrp2bp 4:intergenic:4930412B13Rik-Gm13986 4:intergenic:Atoh1-Gm15534 4:intergenic:Pld5-Gm26104 4:intergenic:Gm14055-Gm14064 4:intergenic:Gm24929-Gm9892 4:intergenic:Luzp2-Gm26482 4:intron:Phlpp1 4:intron:Gm5177 4:intergenic:Adam28-Stc1 4:intergenic:Gm26468-Rpsa-ps10 4:intergenic:Gm22774/Gm15590-Tulp4 4:intron:Dpyd 4:intergenic:Gm22469-Gm23909 4:intergenic:Gfod2-Ranbp10 4:intergenic:Gm24720-Tshz1 4:intergenic:Agbl1-Gm23239 4:intergenic:4930564D02Rik-1700095B22Rik 4:intergenic:4930512H18Rik-Palld 4:intron:Vmn2r90 4:intergenic:Gm22495-Dtna 4:intron:Znrf3 4:intron:Sh2d4b 4:intergenic:Gm4822-Tpm3-rs7 3:intron:Clpb 3:intergenic:Gm26595-Gm26622 4:intergenic:4930554G24Rik-Gm12006 4:intergenic:Tmem37-Dbi 3:intergenic:Gm14082-Gm11644 4:intergenic:Gm26619/Gm10260-Arhgef28 4:intergenic:Lrrtm4-Gm26264 4:intergenic:Gm4889-Dusp4 4:intergenic:Gm14055-Gm14064 4:intergenic:Gm24462-C230034O21Rik 4:intergenic:Mb21d2-Hrasls 4:intergenic:Zfp157-Gm5564 4:intergenic:Gm7204-Dppa4 4:intergenic:Fam92a-Gm11847 3:intergenic:Ttll7-Gm23131 4:intergenic:H2afy3/Pvt1-Pvt1 4:intergenic:Gm11804-8430436N08Rik 4:intergenic:Tacr3-Gm24048 4:intergenic:Gm14534-5730405O15Rik 3:intergenic:Nyap2-Gm5530 4:intron:Kcnq3 4:intergenic:Gm22219-Mc4r 4:intron:Rhobtb1 4:intergenic:Gm22917-Gm26245 4:intron:Pramel7 4:intergenic:Wwp2-Psmd7 4:intron:Lzts1 3:intron:Trpc4 4:intron:Gm15246 4:intron:Slc36a3 4:intergenic:Med13l-n-R5s175 4:intergenic:Lrpprc-1110020A21Rik 4:intergenic:Pbx1-Pbx1/AC096625.1 4:exon:Fam76a 4:intron:Tbc1d10c 4:intron:Col28a1 3:intergenic:Rnf180-Htr1a 4:intergenic:Rasgrp1-Gm13982 4:intergenic:Frmd4b-Gm25852 3:intergenic:Gm9381-Ndst4 4:intron:Ppp1r21 4:intron:Dopey1 4:intron:Gm11762 4:intergenic:Chst3-Gm23488 4:intron:Bend3 4:intergenic:Gm22659-Gm22805 4:intergenic:Mipep-Tnfrsf19 4:intergenic:Pxdn-Tpo 4:intron:Grlf1 4:intron:Nrxn3 4:intergenic:Gm806-Mir683-1 4:intergenic:Gm15425-Pde10a Off-target primers show all... show less...
286 / rev	`AGGTAAGTGATAGATCCACA GGG` `.................... ...` Cloning / PCR primers	78	89	76	42	75	85	0 - 0 - 1 - 19 - 107 0 - 0 - 0 - 0 - 2 127 off-targets	3:intergenic:Trib1-Gm7083 4:intergenic:Gm12886-Gm12880 4:intergenic:Gm24635-Arrdc3 4:intergenic:Olfr850-Olfr851 4:intergenic:Gm23823-Enox1 3:intron:Dmc1 4:intron:Dcun1d4 4:intergenic:Gm6430-Slco6c1 4:intergenic:Txnrd1-Gm4799 4:intergenic:Gm11417-Asic2 4:intergenic:Ikzf2-Spag16 3:intergenic:Gm23594-Il20rb 3:intergenic:Gm9726/mmu-mir-8099-2-Gm26055 4:intron:R3hdm2 4:intergenic:Gm23324-Gm24680 4:intron:Pip5k1b 4:intron:Asic2 4:intron:Macrod2 3:intergenic:Tspan9-Gm26338 4:intron:Ccdc171 4:intron:Lrig3 4:intergenic:Gria4-Gm24896 4:intergenic:Tas2r140-2700089E24Rik 3:intergenic:Dnhd1-Mrps36-ps2 4:intergenic:1700040L02Rik-Tmem26 4:intron:Nos1 4:intergenic:Mir466f-4-Irx1 4:intergenic:Gm14628-Gm14604 4:intergenic:Gm26486-Rbpj 4:intron:Efna5 4:intron:Myo1b 4:intergenic:4930512M02Rik-Gm11999 4:intergenic:Gm16160-Ngf 4:intergenic:Thsd4-Lrrc49 4:intergenic:Vmn2r93-Vmn2r95 4:intron:Ralgapa1 4:intergenic:Sh3bp4-Agap1 4:intron:Sorcs3 4:intergenic:Khdrbs3-Gm24232 4:intergenic:Gm24767-Gm17733 4:intergenic:Gm12678-Gm12493 4:intron:Arhgap25 4:intron:Gm11992 4:intergenic:Uggt1-Neurl3 4:intergenic:Gm9959-Gm9386 4:intron:Kcnh7 4:intron:Jakmip2 4:intron:Atp8b1 4:intron:Nfyc 4:intergenic:Gm23038-4930570G19Rik 4:intergenic:Gm12665-Gm24255 4:intergenic:Mef2a-Lrrc28 4:intergenic:Gm4487-Gm24073 4:intergenic:Gm16325/4930503B20Rik-Gm16325 4:intron:Atg7 3:intergenic:Gm26197-Akap11 4:intron:Sox5 4:intergenic:Fbxo11-Gm4832 4:intron:Ctbp2 4:intergenic:Rbms1-Gm22712 4:intergenic:Sipa1l2-Map10 4:intergenic:Gm9884-Gm24064 4:intergenic:Cmpk2-Gm9866 4:intron:Arhgef3 4:intergenic:Bend5-Agbl4 4:intron:Rims1 4:intergenic:Gm24132-Gm25160 4:intergenic:Gm25550-Cdh9 4:intergenic:Gm4487-Gm24073 4:intron:Pcdh11x 3:intron:Grhl1 4:intergenic:Gm10543-Gm25578 4:intergenic:Gm11862-Gm23975 4:intergenic:Zbtb10-Gm22604 4:intergenic:Gm12226-Gm12225 3:intron:Sap130 4:intergenic:Gm16320-Gm26260 4:intron:Dscaml1 4:intergenic:Gm23534-Gm22727 3:intergenic:Cnot7/Zdhhc2-Cnot7 4:intergenic:Zfp825-Erap1 4:intergenic:5830418P13Rik-Bfsp2 4:intergenic:Gm25096-Gm25634 4:intron:Nckap5 4:intron:Grm7 3:intergenic:Rap1b-Mdm1 4:intron:Camta1 3:intergenic:Gm13458-Gm13455 3:intergenic:Gm5771-Gm5409 4:intergenic:Grik2-Ascc3 3:intergenic:Gm26177-Gm25602 3:intergenic:4930441J16Rik-Lnp 3:intron:Cntn1 3:intron:Otud7b 4:intergenic:Gm22120-Vrk1 4:intergenic:Gm15663-Mettl25 4:intron:Sec24d 4:intron:Gprin3 4:intergenic:Gm24183-Gm22597 4:intron:Synpo2l 4:exon:Fermt3 4:intergenic:Clic5-Runx2 4:intron:Abhd2 4:intron:Mtmr3 4:intergenic:Zc3h13-Siah3 4:intergenic:Gm25644-Rpl31-ps18 4:intergenic:Gm23339-Gm25570 4:intergenic:Gm24986-Gm17032 4:intergenic:Gm12111-Rpsa-ps4 4:intron:Dock7 4:exon:G630030J09Rik 4:intergenic:Mir31-Gm12603 4:intron:Map4 4:intergenic:Col13a1-Gm5424 4:intergenic:n-R5s30-Rbfox1 4:intergenic:Gm3985-Gm6877 4:intergenic:Dopey2-Morc3 4:intergenic:Vrk2-Gm12071 4:intergenic:Gm11263-Gm11409 4:intergenic:Olfr372-Olfr373 2:intergenic:Dlx2-Gm1631 3:intron:Kif13b 4:intergenic:Gm10051-Gm15627 4:intron:1700001L19Rik 4:intergenic:Gh-Cd79b 3:intron:Mfsd4 4:exon:Lao1 Off-target primers show all... show less...
291 / fw	`AGGGTATTAAGTTATCCCTG TGG` `.................... ...` Enzymes: BspPI, MflI, LpnPI, NdeII Cloning / PCR primers	78	86	76	34	84	82	0 - 0 - 1 - 16 - 106 0 - 0 - 1 - 2 - 1 123 off-targets	4:intergenic:Gm24080-Gm22104 4:intergenic:Psma6-Nfkbia 4:intergenic:Slc22a3-Slc22a2 4:intergenic:Mir3963-Eltd1 3:intron:Efcab4b 4:intergenic:Gm23665-Csmd3 4:intergenic:Adamts5-Gm25715 4:intron:Psg18 4:intron:Psg26 4:intron:Psg28 3:intron:Zbtb38 4:exon:Pias1 4:intron:Mybpc1 4:intergenic:Samd4-mmu-mir-378c 3:intergenic:RP24-338F2.1-Tac1 4:intron:9330158H04Rik 4:intergenic:Gm14781-Mageb5 4:exon:Fchsd2 3:intergenic:Gm22862-Gm22598 3:intergenic:Gap-Gm25431 4:intergenic:Tspyl5-Mtdh 4:intergenic:Gm23022-Rab28 4:intergenic:Ppp4r2-Pdzrn3 4:intergenic:Defa21-Gm23142 4:intergenic:Defa22-Gm26240 4:intergenic:Gm15293-Defa-ps5 4:intergenic:Gm15315-Defa-ps9 4:intergenic:Gm4791-Gm22286 4:intron:Camk1d 4:intergenic:Gm25550-Cdh9 4:intron:Kcnma1 3:intergenic:4930555J06Rik-Scin 4:intron:Ptprr 4:intergenic:Eya4-Rps12 3:intron:RP23-304I16.1 4:intergenic:Gm25389-Lrrk2 4:intergenic:Gm26327-Gm6851 4:intergenic:Mgat4c-Gm26988 4:intergenic:AC175117.1-Hnf4g 4:intron:Cdh6 4:intron:Map2k5 3:intron:Pfdn1 4:intergenic:Gm11499-Gm11500 4:intron:Ptprz1 4:intergenic:Gm27025-Gm25700 4:intergenic:Gm6963-Fyn 4:intergenic:Efcab5-Ssh2 4:intron:4931429P17Rik 4:intergenic:Gm5257-mmu-mir-6343 4:intron:Mrps21 3:intron:Itga9 4:intron:Pebp4 4:intron:Arhgap8 4:intergenic:AW209491-Gm6556 4:intergenic:BC042782-Gm12708 4:intergenic:Fbxo34-Atg14 3:intergenic:Gm14712-Magea7-ps 3:intergenic:Epha3-Csnka2ip 4:intergenic:Adamts5-Gm25715 4:intergenic:Trav5d-4-Trav6d-7 4:intergenic:Trav5n-4-Trav6n-7 4:intergenic:Col13a1-Gm5424 4:intron:Ap3b1 4:intron:Parvg 2:intergenic:Gm2308-Vmn2r60 4:intergenic:Gm26029-Gm22225 4:intergenic:Gm11826-Gm11825 4:intron:Lrrc69 4:intergenic:mmu-mir-6390-Gm10076 4:intron:Cpeb1 4:intergenic:Dnah5-Mir3964 4:intron:Prkcq 4:intron:Magi2 4:intergenic:Klk1b3-Klk1b4 4:intergenic:Klk1b27-Klk1b21 4:intergenic:Klk1b11-Klk1b26 3:intergenic:Gm9434-Nyx 4:intergenic:AC175117.1-Hnf4g 4:intergenic:Gm13326-Gm13325 4:intergenic:Enah-Srp9 4:intergenic:Trank1-Dclk3 4:intron:Pkp4 4:intergenic:Nrg3-Gm20642 4:intergenic:Gm829-Gm12410 4:intron:Mpped1 4:intergenic:Gm24202-Gm8100 4:intergenic:Fbxo34-Atg14 4:intron:Rsu1 4:intergenic:Gm12639-Gm12641 4:intergenic:Cstf2-Cstf2/Nox1 4:intergenic:4732465J04Rik-Gm24433 4:intron:Senp7 4:exon:Fgf12 4:intron:Hecw2 4:intergenic:Gm25925-Hpcal1 3:intergenic:Ptprk-Themis 4:intron:Rpap2 4:intergenic:Nrbf2-Egr2 4:intron:Lama2 4:intergenic:Olfr664-Usp17le 4:intergenic:Gm23225-Gm24127 3:intron:Cc2d1a 4:intergenic:Tm2d3-Gm7551 4:intergenic:Cyp1b1-Atl2 3:intergenic:Gm24522-4930558G05Rik 4:intron:Ophn1 4:intergenic:Gm16409-Gm22164 4:intron:Pi15 4:intergenic:Tmx1-Gm24474 4:intergenic:Gm21070-Jrkl 4:intergenic:Gm14555-Rhox12 4:intergenic:Cdh18-Gm5803 4:intergenic:Pdgfd-Ddi1 4:intergenic:Icos-Rpl17-ps1 4:intron:Lrrn1 4:intron:Fhod3 4:intergenic:Gm2348-Slc1a3 4:intergenic:Gm5742-Gm22223 4:exon:Klc1 4:intergenic:Gm10650-Gm22293 3:intron:Nyap2 4:intergenic:Ptbp2-Gm24744 4:intergenic:Klk1b8-Klk1b1 Off-target primers show all... show less...
629 / fw	`GTATGTTGTGCCCTCAACAA TGG` `.................... ...` Enzymes: HinfI, PpsI Cloning / PCR primers	77	92	53	62	74	44	0 - 0 - 2 - 20 - 103 0 - 0 - 0 - 2 - 3 125 off-targets	3:intergenic:4930595M18Rik-Gm8822 4:intron:Catsperb 4:intron:Kazn 3:intergenic:Gm4945-Trerf1 4:intergenic:Tmprss15-Rps19-ps12 4:intron:Col4a4 4:intergenic:Gm26468-Rpsa-ps10 4:exon:Klf16 4:intron:Pnkd 4:intergenic:Gm5409-Gm10334 4:intergenic:Ranbp6-Il33 3:intron:Slc6a11 3:intergenic:Gm23950-Gm11218 4:intergenic:Kcnh5-Rhoj 4:intergenic:Gm6956-Gm5934 4:intergenic:Gm5860-Nfib 3:intergenic:Fam169b-Gm5334 4:intron:Ralgapa2 3:intron:Cntnap5c 4:intergenic:Gm11499-Gm11500 4:intergenic:Mir1899-Trpc6 4:intergenic:Gm4784-Gm4991 3:exon:Irf9 4:intergenic:Gm14336-Gm14337 4:exon:1700003E24Rik 4:exon:1700084M14Rik 4:exon:Gm9059 4:exon:BC061195 4:intergenic:Plk2-Actbl2 4:intergenic:Gm27023-Gm25800 4:intergenic:Bhlhb9-Gm15016 4:intron:Epb4.1l5 4:intron:Grik4 4:intergenic:Gm7224-Vmn2r59 4:intergenic:Gm5449-Drd1a 4:intergenic:3830408C21Rik-Apoo-ps 4:intergenic:Gm4759-Gm8995 4:intergenic:Dgat1-Scrt1 3:intergenic:Spaca1-Gm25623 4:intergenic:Gm16325/4930503B20Rik-Samd13 4:intergenic:Gm10232-Gm16569 3:intergenic:Cnga2-Magea4 4:intergenic:Cyp7b1-Gm6369 4:intergenic:Polk-Gm17622 3:intergenic:Lrriq1-Slc6a15 2:intergenic:Zfp420-Zfp27 2:intron:Bdp1 4:intergenic:Tmprss15-Rps19-ps12 4:intergenic:Ackr3-5S_rRNA 4:intergenic:D630008O14Rik-Bcl2 4:intergenic:Epm2a-Gm9797 4:intergenic:Fxyd4-Gm4875 4:intergenic:Agr3-Agr2 4:intergenic:PVT1_3-Ndufa4 3:intergenic:Plod2-Gm9621 4:intron:Gm26907 4:exon:1700120B22Rik 4:intergenic:Gm17421-Ighm 4:exon:Srsf10 3:intron:Stpg2 4:intergenic:Gm22251-Zfp521 3:intergenic:Cdh12-AC125320.1 4:intergenic:Gm23260-Nr3c2 4:intergenic:Gm25460-Gm5263 4:exon:Sc4mol 4:intergenic:Fam135b-Gm22519 4:intergenic:Gm26104-Cep170 4:intergenic:Gm26054-Xylt1 4:intergenic:H13-Id1 3:intergenic:Gm11412-n-R5s187 4:exon:Gramd1b 4:intergenic:4930555K19Rik-Oxr1 3:intergenic:Ighv7-1-Ighv7-2 4:intron:4933411K20Rik 4:intergenic:Nat10-Caprin1 4:intergenic:Gm11591-Gm11587 4:intergenic:Slc5a10-Fam83g 4:exon:Tpm4 4:intergenic:Gm15693-Gpr128 4:exon:Gm7809 3:intergenic:4930594M17Rik-Gm26012 4:intergenic:Daam2-Kif6 3:intergenic:Unc5b-mmu-mir-6408 4:intergenic:Actr1b-Zap70 4:intergenic:Cdh10-Gm25976 4:intron:Pcsk5 4:intergenic:Cul4b-Mcts1 4:intron:Tbp 4:intergenic:Gm22702-Gm11499 4:intergenic:Arid5b-1700040L02Rik 4:intergenic:Gm24696-Myc 4:intergenic:Abcg2-Herc3 4:intergenic:Lhfp-Nhlrc3/Gm16206 4:intron:Dnah3 4:intergenic:4833424O15Rik-Snx7 4:intergenic:Ttll7-Gm23131 4:intergenic:Gm22331-Gm24937 4:intergenic:Gm23891-1700018B24Rik 4:intergenic:B930078G14Rik-Fam155a 4:intergenic:Dmd-Gm22580 4:intergenic:Celf2-Gm13210 4:intergenic:Gm23024-Gm24901 4:intergenic:Defa-ps13-Defa-ps14 4:intron:Unc5d 3:intergenic:Zeb1-Arhgap12 4:intron:Zc3h13 4:intron:Ppp4r4 4:intergenic:Gm23534-Gm22727 3:intergenic:Cpq-Gm24476 4:intron:Cdh11 3:intergenic:Lbx1-Btrc 4:intron:Slc22a1 4:intergenic:Olfr1095-Olfr1096-ps1/Gm13723 4:intergenic:Gm24350-Gm13464 4:intron:Pdgfd 4:intergenic:4930595M18Rik-Gm8822 4:intron:Cdkal1 4:intron:Pknox1 4:intergenic:Gm6132-Gm26018 4:intergenic:1700001G17Rik-Rab23 4:intergenic:Dis3l-Megf11 4:intergenic:Oprk1-Gm22463 4:intron:Lama4 4:intergenic:Olfr668-Olfr669 4:intergenic:1110036E04Rik-Scarletltr Off-target primers show all... show less...
244 / fw	`GTACATCAATAAGTCATTAC TGG` `.................... ...` Inefficient Enzymes: HincII, Hpy166II, Bse1I Cloning / PCR primers	76	93	45	26	71	88	0 - 0 - 8 - 12 - 83 0 - 0 - 1 - 1 - 2 103 off-targets	2:intergenic:Gm11986-U7 4:intron:Slc5a4a 4:intergenic:Gbe1-Gm24968 4:intergenic:Aldh1a1-Aldh1a7 3:intergenic:Gpr125-mmu-mir-8117 4:intergenic:Tusc3-Msr1 4:intron:Esr1 4:intergenic:Gm24070-Gm24994 4:intergenic:Pcdh20-Gm25886 4:intergenic:Gm9006-4930465M20Rik 4:intergenic:Olfr1006-Olfr1008 4:intergenic:Rit2-Gm23283 4:intergenic:Gm24192-Gm12132 4:intergenic:Gm12641-Gm12642 4:intron:Tecrl 4:intron:Sgcd 3:intergenic:Has2os-Gm26178 4:intergenic:Gm11894-Gm25975 4:intergenic:Med13l-n-R5s175 4:intergenic:Fndc1-E430024P14Rik 4:intergenic:AC112986.1-Tsn 4:intron:Ptk7 4:intron:Adcy9 4:intergenic:Tusc1-Gm12649 4:intergenic:Gm5082-Adtrp 4:intergenic:Cyyr1/Gm2541-Adamts1 4:intergenic:Epha6-Gm24755 4:intergenic:Svs5-Slpi/mmu-mir-7678 3:intergenic:Gm26076-Gm25519 4:intergenic:Diap3-Gm23278 4:intergenic:Pou2f3-rs1-Serpinb12 4:intron:Il1rap 4:intron:Kcnj6 3:intergenic:Gm11918-Gm11919 4:intergenic:Ces5a-Gm26843 4:intergenic:Gm25133-Gm24770 4:intron:D6Wsu163e 4:intergenic:Gm25505-Gm25377 4:intergenic:Olfr481-Olfr482 4:intergenic:Gm24968-Gm22797 4:intergenic:Gm14263-Gm14266 4:intergenic:AC112986.1-Tsn 4:intergenic:AC112986.1-Tsn 4:intron:Shroom4 3:intron:Atg4c 4:intron:Lrrc4c 2:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Ihh-Nhej1 3:intergenic:Gm7224-Vmn2r59 4:intron:Esrrg 4:intergenic:Zdhhc20-Micu2 4:intergenic:Gm16477-Gpr26 4:exon:Xkrx 2:intergenic:Cox7c-Gm27025 3:intergenic:1600029D21Rik-Pts 4:intergenic:Gm26662-Kirrel3 4:intergenic:Gm6632-Gm22308 4:intergenic:Dleu2-Gm26969 4:intergenic:1700018B24Rik-Gm23511 4:exon:Hook1 4:intergenic:Fam194a-Siah2 3:intergenic:Kcnc2-Atxn7l3b/Gm26596 3:intergenic:Olfr1053-Gm13719 2:intergenic:Gm25297-Dlk1 4:intergenic:Isg20-Gm26633 4:intergenic:Gm25522-Gm25587 2:intron:Strbp 2:intergenic:Gm5620-Zic1 4:intron:Anks1b 3:intergenic:Gm25011-Gm23680 4:intergenic:Aff3-Gm16152 4:intergenic:Suclg2-Fam19a1 4:intron:Creb5 3:intergenic:Gm22230-Gm23558 4:intergenic:Kcnh7-Gm23503 4:intron:Malt1 2:intergenic:Gm24834-Gm7125 4:intron:Fam149b 2:intergenic:Gm5593-Vmn2r60 4:intergenic:Gm16029-Gdnf 4:intergenic:Bbs9-Gm16165 4:intron:Herc2 4:intron:Pik3r6 4:intron:Bai3 4:intergenic:4930584F24Rik-Dpp6 4:intergenic:Syn2-Gm17482 3:intergenic:Gm23558-Galc 4:intergenic:Gm13751-Gm13754 4:intergenic:Gm24470-Gm14741 4:intergenic:Ces5a-Gm26843 4:intron:Psmd1 4:intergenic:Ppp1r2-ps2-Nup210 4:intergenic:Gm6268-Gm22364 4:intron:Edil3 4:intergenic:Mir5127-Gm17383 4:intergenic:B130024G19Rik-Gm7656 4:intergenic:Gm26068-Atrnl1 4:intergenic:Sec16b-Brinp2 4:intron:Nat3 4:intergenic:Gm6741-Gm15404 4:intron:Ubl7 4:intron:Cps1 4:intergenic:Sox4/Gm26735-A330102I10Rik Off-target primers show all... show less...
271 / fw	`CTTTGTACTTTAGCACAATA AGG` `.................... ...` Cloning / PCR primers	75	85	40	33	59	89	0 - 0 - 2 - 23 - 158 0 - 0 - 0 - 2 - 6 183 off-targets	4:intergenic:Gm23185-Gm23848 3:intergenic:Gm7076-Rpl7l1-ps1 3:intergenic:Gm24728-Lmo4 3:intron:Nav3 4:intron:Mprip 4:intergenic:RP24-308I2.1-RP24-86O15.2 3:intron:Aox3 4:intron:Stag1 4:intergenic:Pax6os1-Pax6 4:intron:Csmd1 3:intergenic:Gap-Vmn1r-ps151 4:intron:1700034P13Rik 4:intergenic:Tnfrsf21-Gpr110 4:intergenic:Ptpre-5830432E09Rik 4:intergenic:Gm25708-Gm5262 4:intergenic:AC149090.1-Gap 4:intergenic:Gap-Gm10232 4:intergenic:Gm27023-Gm25800 4:intron:Asxl3 4:intergenic:Trim2-Fhdc1 4:intron:Slc6a5 4:intergenic:1700028N14Rik/Map3k14-Arhgap27 4:intron:Gpr39 4:intergenic:Gm5334-Arrdc4 4:intergenic:Gm23881-Gm3534 3:intron:Elovl6 3:intron:Aff3 4:intron:Msmb 4:intergenic:Gm22016-Cdc73 4:intergenic:Hs3st4-Gm15339 4:intron:Lrp2 4:intergenic:Gm11925-Gm11924 4:intergenic:Gm13157-Gm20707 4:intron:Cdk8 4:intron:Spsb1 4:intron:Baalc 3:intergenic:Gm4784-Gm4991 4:intergenic:Gm11996-Gm11997 4:intron:Tmem154 4:intergenic:Gm5468-Myo10 3:intergenic:Bmi1-Gm13334 4:intergenic:Gm13379-Gpr158 4:intron:Kcnj3 4:intergenic:Gm25442-Gm25696 4:intergenic:Gm24861-Chodl 4:intergenic:Gm23061-Ostf1 4:intergenic:Cdh18-Gm5803 4:intron:Cdkal1 4:intergenic:Zranb2-Ptger3 3:intergenic:Gm23860-Gm11800 4:intergenic:Gm8521-Gm20918 4:intron:Epas1 4:intergenic:Gm25985-Gm6404 4:intron:Zfp820 4:intron:Slc22a28 4:intron:Slc22a30 4:intergenic:RP23-2C22.3-Sult2a6 4:intergenic:Gm12695-Gm23890 3:intergenic:4930594M17Rik-Gm26012 4:intergenic:Gpc5-Gpc6 4:intergenic:Gm22183-mmu-mir-378d 4:intergenic:Frzb-Gm13687 4:intron:4933406I18Rik 3:intergenic:Cd180-Mast4 4:intergenic:Gm11911-Gm11907 4:intron:Agbl4 4:intron:0610012H03Rik 3:intron:Hrnr 4:intron:Lrrfip2 4:intergenic:Pdzrn3-Gm9871 4:intergenic:Fezf2-Cadps 4:intergenic:Cdh6-Gm9630 4:intron:Cntn5 4:intergenic:Gm2383-Gm6216 4:intron:Stxbp5l 4:intron:Pum1 4:intergenic:Kif20b-Gm24006 4:intron:Fam135b 4:intron:Lmln 4:intergenic:AC112986.1-Tsn 4:intergenic:Gm23848-Trps1 4:intergenic:Sdc3-Gm12970 4:intergenic:Pde4b-Gm24869 4:intron:Thsd7a 4:intergenic:Gm20415-Vmn2r-ps69 3:intergenic:Gm24232-Gm23987 4:intron:Cdh11 4:intergenic:Pde4b-Gm24869 4:intergenic:Akr1c12-Akr1c6 4:intergenic:Lama2-Gm10145 4:intergenic:Ermard-Gm5091 3:intergenic:Itih5l-ps-Gm8424 4:intergenic:Trim27-Gpx5 4:intergenic:Atp1b4-Gm40 4:intron:Nol4 4:intergenic:Rab3c-Gm5455 4:intergenic:BC030500-Gm24847 3:intron:Uhrf1bp1l 4:intergenic:RP23-302F9.3-RP23-394M5.2 4:intergenic:Nrsn1-Tpi-rs8 4:intergenic:Dcc-Gm25509 4:intron:Npas3 4:intron:Ssbp2 4:intergenic:Tvp23a-Ciita 4:intron:1700081H22Rik 4:exon:Neb 3:intergenic:Gm23203-Gm23447 4:intergenic:Gm25248-Gm26326 4:intergenic:Gm23511-Gm22480 4:intergenic:Asb9-Gm15216 4:intron:Mapk10 4:intergenic:Gm22337-Gm25347 4:intergenic:Igkv17-127-Igkv14-126 4:exon:Dnhd1 4:intergenic:Gm12026-Gm12027 4:intergenic:Gm26137-Gm23733 4:intergenic:Begain-Gm22234 4:exon:Zfp407 4:intergenic:Gm15645-Gm4070/Gm20663 4:intergenic:RP23-142E18.5-Gvin1 4:intergenic:Olfr983-Olfr984 4:exon:Osmr 4:intergenic:Opn4-Wapal 4:intron:Ptprd 4:intergenic:Qtrtd1-2610015P09Rik 4:intergenic:Gm11381-Mylk4 2:intergenic:Gm5594-Vmn2r-ps59 3:intergenic:Gm22521-Gm25702 4:intergenic:Gm19301/Fam216b-Mir1971 3:intergenic:Nell1-Ano5 4:exon:Arhgef12 4:intergenic:Ces5a-Gm26843 4:intron:Lrp1b 4:intron:Vps13b 4:exon:Ube3b 4:intergenic:Gm1815-Zfp353-ps 3:intergenic:Gm8349-Arhgef26 3:intergenic:Rpsa-ps10-Mir3963 4:intron:Lrrc7 4:intergenic:Tspyl5-Mtdh 4:intron:Gpr155 4:intergenic:Gm13487-Gm13485 4:intergenic:Slc16a7-Lrig3 4:intron:Tm2d1 4:intergenic:Gm26913-Gm4076 4:intergenic:Gm25664-Slc35f1 4:intergenic:Cypt15-Gm14550 4:intergenic:Gm13468-Gm13481 4:intergenic:F13a1-Ly86 3:intergenic:Rdh14-Pgk1-rs7 4:intergenic:1700021A07Rik-1700020N01Rik 4:intergenic:Rarb-Thrb 4:intergenic:Aqp9-Aldh1a2 4:intron:Tmem45a 4:intergenic:Gm26235-Gm25392 2:intergenic:Gm24127-Pcdh9 3:intergenic:Ear14-Ear11 4:intergenic:Gm23022-Rab28 4:intergenic:Ubr5-Odf1 4:intergenic:Gm16428-Gm7916 4:intergenic:Mettl24-Cdc40 4:intergenic:Rps19-ps5-Rrm2b 4:intergenic:Gm5849-4930529C04Rik 4:intergenic:Gm24297-Gm22911 4:intergenic:Gm24256-Grik2 4:intergenic:Gimap5/RP24-315D19.2-Gimap3 4:intergenic:Gm22503-Gm24271 4:intergenic:Mir690-Mb21d2 4:intergenic:Large-Gm23255 4:intron:Grid2 4:intergenic:Aggf1-Crhbp 4:intergenic:Kdm6a-n-R5s5 4:intergenic:4930505A04Rik-Acyp2 4:intergenic:Gm10247-Grin2a 4:intergenic:Nipal2-Kcns2 4:intergenic:Gm11845-Tmem64 4:intergenic:Grik2-Gm26543 4:intergenic:Atg14-Ktn1 4:intergenic:Tmsb4x-Tlr8 4:intergenic:Apol11b-Apol7e 4:intergenic:Gm25267-Il15 4:intron:Cisd2 4:intergenic:Rps18-ps2-Gm23423 Off-target primers show all... show less...
479 / fw	`TCATATCCTTCATGGTTTAC AGG` `.................... ...` Inefficient Enzymes: Hpy166II Cloning / PCR primers	74	88	29	34	63	81	0 - 0 - 2 - 12 - 137 0 - 0 - 0 - 0 - 9 151 off-targets	3:intergenic:Gm5594-Vmn2r-ps59 3:intergenic:Gm14775-Il1rapl1 4:intron:Map2k5 3:intron:Snrnp48 3:intergenic:Ptch2-Btbd19 4:exon:B3galt5 4:intergenic:Gm25550-Cdh9 4:exon:Fbxo3/4931422A03Rik 4:intergenic:Gm10038-Gm13874 4:intron:Kirrel 4:intergenic:Gm22005-Nmt2 4:intergenic:Gm13751-Gm13754 4:intergenic:Tle3-Rplp1 3:intergenic:Gm14903-Gm14906 4:exon:Rtl1/Mir431/Mir433/Mir127/Mir434/Mir432/Mir136 4:intron:Rbms3 4:intron:Cstf1 4:intergenic:n-R5s86-Wdr72 3:intergenic:Prkag2-1500035N22Rik 4:intron:Kcnip3 4:intron:Caln1 4:intergenic:Gm13385-Gm13386 4:intron:Socs6 4:intron:Ctps2 4:intergenic:Eif4g2-Galnt18 4:intergenic:Mycn-Gm24208 4:intergenic:Bche-Gm6098 4:intergenic:Gm26006-Gm26139 4:intron:1700081H22Rik 4:intergenic:Gm22308-Cbfa2t2-ps1 4:intergenic:Pid1-Dner 4:intron:Kif12 2:intron:Ntrk2 4:intron:4732491K20Rik 4:intron:Parp8 4:intergenic:Vma21-ps-4930547C10Rik 4:intron:Rft1 4:intergenic:G930045G22Rik-Mir148a 4:exon:Itgal 4:intergenic:Rhox12-Rhox13 4:intergenic:Rhox12-Rhox13 4:intergenic:Ago2-Ptk2 4:intergenic:Irx2-Irx4 4:intergenic:Fat4-Gm9845 4:intergenic:Stx17-Erp44 4:intergenic:2410076I21Rik-Hcn4 4:intron:Kcnc2 3:intergenic:Atp2b2-Gm15083 4:intergenic:Gm23176-Nt5e 4:intergenic:Chst13-Uroc1 3:intron:Nceh1 4:intergenic:Gm26937-Lipi 4:intergenic:Gm20696/Eif4e3-Gpr27 4:intron:Ucp1 4:intergenic:Gm13501-Gm13503 4:intergenic:E430024P14Rik-Tagap 3:intergenic:Lmo4-Hs2st1 4:intergenic:Gm11650-Gm23120 4:intron:Btrc 4:intergenic:Nobox-Tpk1 4:intron:Nrxn3 4:intergenic:Gm22944-RP23-442I7.1 4:intron:Nf1 4:intergenic:Olfr32-Gm4222 4:intergenic:Gm24510-Nrxn1 4:intron:Fam120b 4:intergenic:Gm13518-mmu-mir-195b 4:intron:Pde5a 4:intron:Pgm1 4:intergenic:9130409J20Rik-Gm12299 4:intron:Dab2ip 4:intergenic:Gm16204-Mir383 4:intron:Adam22 4:intergenic:Gm5161-Ephb1 4:intron:Nav2 4:intergenic:Gm24847-Gm26164 4:intergenic:Camk1g-4631405K08Rik/Mir205 4:intergenic:Gm22536-Gm6632 4:exon:Tmem161a 4:intergenic:Gm26766-Gm16136 4:intron:Lrp1b 4:exon:Dctn1 4:intergenic:MIMT1_1-Mir3099 4:intron:Ltbp2 4:intergenic:Galnt14-Ehd3 4:intergenic:Gm11591-Gm11587 4:intergenic:Gm24584-Sfrp2 4:intron:Sox6 4:intron:Tenm2 4:intergenic:Pdgfd-Gm23097 3:intron:Lrp1b 4:intron:E130114P18Rik 4:intron:Dlg2 4:intergenic:Gnai1-Gm17037 4:intergenic:Ncor1/Pigl-Pigl 4:intron:Papolg 4:intergenic:4930449A18Rik-Gm8298 4:intergenic:1700061I17Rik-D3Bwg0562e/Gm22408 3:intergenic:C230034O21Rik/Fat4-Fat4 4:intron:Gpc4 4:intergenic:Gm23907-Efr3a 4:intergenic:4930474N05Rik-Gm24394 4:intron:Ndst3 4:exon:Gpr1 4:intergenic:Gm20319-Gmnc 4:intergenic:Gm14020-Frmd5 4:intron:Mllt1 4:intergenic:Gm9769-Cml2 4:intergenic:Cpa1-Cep41 4:intron:Efemp2 4:intron:Sphkap 4:intergenic:Synpr-Sntn 4:intergenic:Gm25708-Gm5262 4:exon:Yes1 2:intergenic:Gm23892-Gm25382 4:exon:Vsig10 4:intron:Mkl1 4:intergenic:Gm25138-Gm8167 4:intron:Tenm1 4:intergenic:Rpl31-ps23-Ppp1r3b 4:intron:Gm26758 4:intergenic:Gm6910-Gm10324 4:intergenic:AC164297.1-Zfp640 4:intergenic:2410141K09Rik-Gm26806 4:intron:Ppp4r1l-ps 4:exon:Capza2 4:intergenic:Scgb2b17-Gm9162 4:intergenic:Scgb1b7-Gm5329 4:intergenic:Scgb1b7-Gm5329 4:intergenic:Scgb2b15-Gm25817 4:intergenic:Gm5329-Gm9135 4:intergenic:Gm5116-Gm5327 4:intergenic:Gm4991-Gm7134 4:exon:Nfe2l2 4:intergenic:Gm15323-Ankrd55 4:intergenic:Scgb1b29-Gm9146 4:intergenic:Setbp1-Gm25824 4:intergenic:Abcb7-Uprt 4:intron:Rb1 4:intergenic:Gm5215-Colec10 3:intergenic:Gm25899-Ccdc171 4:intergenic:Gm14832-Eda 4:intergenic:Nav3-E2f7 4:intergenic:Rnf144b-4931429P17Rik 4:intergenic:D630033O11Rik-Trim29 4:intergenic:Gm25562-AI593442 4:intergenic:A530053G22Rik-Snca 4:intergenic:Nuf2-Rgs5 4:intergenic:4933406J10Rik-Eftud1 4:intergenic:AC114591.1-Slco3a1 4:intergenic:Gm23022-Rab28 Off-target primers show all... show less...
99 / fw	`ATTGCTCAGGATTGTTGGGA AGG` `.................... ...` Cloning / PCR primers	73	82	59	29	69	72	0 - 0 - 2 - 22 - 154 0 - 0 - 0 - 1 - 0 178 off-targets	4:intron:Dab2ip 4:intergenic:Sv2c-Gm25213 4:intergenic:2610020C07Rik-Gspt1 3:intergenic:Pou6f2-Vps41 4:intron:Zfp521 4:intergenic:Gm13549-Dapl1 4:intergenic:Gm5257-mmu-mir-6343 4:intergenic:Gm24986-Gm17032 3:intergenic:Gm9001-Gm22283 4:intergenic:9230114K14Rik-Gm25601 4:intergenic:Mmd-Hlf 4:intergenic:Gm12160-Gm12161 4:intron:Zbtb20 3:intron:Sdk1 4:intron:Gm14129 4:intron:Krt42 3:intron:Sorcs3 4:intron:Dock4 4:intron:Tcf4 3:intergenic:Nbea-Gm25727 4:intron:Gm1330 4:intergenic:Abcc8-Ush1c 4:intergenic:Gm24005-Gm26362 4:intergenic:Etl4-Gm13362/Etl4 4:intergenic:Gm22382-Gm22169 4:intron:Tnfrsf11a 3:intron:Polr3a 3:intron:Cbs 4:intergenic:Ldlrad1-Lrrc42 4:intron:Larp1 4:exon:Slc16a6 4:intron:Fam65c 4:intergenic:Plekhb2-Gm25096 4:intergenic:Gm25843-Tmeff2 4:intergenic:Gm13575-Gm13577 4:intron:Slit3 4:intergenic:Gm12066-Gm12068 4:intergenic:AL808026.1-Nhsl2 4:intergenic:Pou6f2-Vps41 4:intergenic:Gm11753-Tbc1d16 4:intergenic:Cbfa2t2-ps1-Gm23331 4:intergenic:Gm25344-Tmem232 3:intergenic:Edn3-Gm14617 4:intergenic:Gm23598-Abhd12 4:intergenic:Gmnc-Ostn 4:intron:Crabp1 4:intergenic:Sspn-Itpr2 4:intergenic:Prep-Popdc3 4:intergenic:mmu-mir-6393-Zdhhc25 3:intergenic:Lmo4-Hs2st1 4:exon:4930503H13Rik 4:intron:Phc3 4:intergenic:Otp-Wdr41 3:intron:Opcml 4:exon:Hmha1 3:intergenic:Gm5594-Vmn2r-ps59 4:intron:Atg2b 4:intergenic:Rasa1-Cox7c 4:intron:Tgfb2 4:intergenic:Rpp40-Ppp1r3g 4:intergenic:Gm25813-Gm23666 3:intergenic:Gm23790-Gm5422 4:intergenic:Gm12010-Gm12011 4:intergenic:Abca12-Atic 4:intron:Rin2 4:intergenic:Gm13865-Vav3 4:intergenic:Gm26651-Diras2 4:exon:Igf2 4:intergenic:Gm14602-Dcaf12l1 4:intron:Cdh23 4:intron:Nvl 4:intergenic:Gm15476-E330021D16Rik 3:intergenic:Gm14838-Gm6206 4:intron:Aox3 4:intergenic:Nfib-n-R5s188 4:intergenic:Nox3-Gm23186 4:exon:Trim12c/Trim34a/U6 4:intergenic:Casc4-Gm23983 3:intergenic:Got1-Gm20467 4:exon:Wnt6 4:intergenic:Igkv15-103-Igkv14-100 4:exon:C2cd3 4:intergenic:Tpbg-Ube2cbp 4:intergenic:Pabpc2-Yipf5 4:intergenic:Cyp3a13-Gjc3 4:intergenic:Gm23437-Gm24818 4:intron:Slc9a3r1 4:intergenic:Tmtc1-Rps4y2 3:intergenic:Fam3c-Gm8927 4:intergenic:Gm26361-Rprd2 4:intergenic:Gm8312-1700036O09Rik 4:intergenic:1700025G04Rik-Tsen15 4:intergenic:Olfr60-Olfr530 4:intergenic:Gm25660-Slc27a6 4:intergenic:Gm9923-Gm24984 4:intergenic:Gm13851-9430029A11Rik 4:intergenic:Gm10247-Grin2a 4:intergenic:Gm15636-Eml1 3:intergenic:Palm2/Gm20459-Gm20459/Palm2 3:intergenic:Tmco3-Tfdp1 4:intron:9530036O11Rik 4:intergenic:Vmn1r30-Vmn1r-ps21 4:intergenic:Gm15501-Gm5899 4:intergenic:Gfra2-Gm9192 4:intergenic:Minpp1-Papss2 4:intergenic:Fgf9-Gm25614 4:intergenic:Dll4-Chac1 4:intron:Irf2 4:intergenic:Gm22145-Tln2 4:intergenic:Robo1-Gm25699 4:exon:Pcnx 4:intergenic:Plekhf1-Pop4 4:intergenic:Clec14a-Gm17529 4:intergenic:Cdh10-Gm25976 4:intergenic:Gm21340-Gm21851 4:intergenic:Ssty2-Gm21114 4:intergenic:Gm21333-Gm21344 4:intergenic:Gm21573-Gm20803 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm21715-Gm21784 4:intergenic:Gm20815-Gm21292 4:intergenic:Gm21302-Gm21740 4:intergenic:Gm21257-Gm20791 4:intergenic:Gm21745-Gm21184 4:intergenic:Gm20852-Ssty2 4:intergenic:Gm21242-Gm21248 4:intergenic:Gm21163-Gm21180 4:intergenic:Gm21198-Gm20808 4:intergenic:Gm21777-Gm21792 4:intergenic:Gm21151-Gm21160 4:intergenic:Gm20865-Gm21065 4:intergenic:Gm20816-Gm20818 4:intergenic:Gm21524-Gm21672 4:intergenic:Gm21201-Gm20853 2:intergenic:4930480K15Rik-Nrxn1 4:intergenic:Gm15062-Gm4918 2:intergenic:Gm7224-Vmn2r59 4:intergenic:BC030500-Gm24847 4:intron:Ppargc1a 4:intron:Klhl29 4:intergenic:Gm22326-Sv2b 3:intergenic:E130304I02Rik/Zfp507-Gm10166 4:intergenic:Gm20822-Gm21809 4:intergenic:Gm20828-Gm20812 4:intron:Ckmt2 4:intergenic:Gm25258-Hao1 4:exon:Slit1 4:intergenic:Gm10275-Cenpw 3:intergenic:Kcnip1-Kcnmb1 4:intron:Kcnh8 4:exon:Mx2 4:intron:Rhbdd3 3:intergenic:Gm4868-Gm23151 4:intron:Adamts6 4:intergenic:Gm23315-Rprm 4:intergenic:Slc30a10-Lyplal1 4:intergenic:Lmx1a-Gm20711 3:intergenic:Cacna1e-Gm9530 4:intron:Hmgxb4 4:intergenic:Gm94-Spink5 4:exon:Arid4b 4:intron:Ttc23l 4:intergenic:Cd97-Gm22024 4:intron:Itpr3 4:intergenic:Olfr721-ps1-Slc4a7 4:intron:1700109G15Rik 4:intergenic:Trh-Prickle2 4:intergenic:Gm25200-Cdh13 4:intergenic:Gm23537-Gm25003 4:intergenic:Gm25672-Barhl2 4:intron:Pvrl1 4:intron:Sec24b 3:intergenic:Gm2308-Vmn2r60 4:intergenic:Gm11415-6030471H07Rik 4:intergenic:Cdk6-Gm17590/Cdk6 4:intergenic:Galnt9-Noc4l 4:intergenic:1700009N14Rik-Gm12385 4:intergenic:Gm23970-Etl4 Off-target primers show all... show less...
548 / rev	`CTACCATATTTCTCCTGGCT AGG` `.................... ...` Enzymes: XcmI, StyD4I, BstNI, MaeI Cloning / PCR primers	73	86	45	34	70	77	0 - 0 - 1 - 16 - 135 0 - 0 - 0 - 5 - 8 152 off-targets	4:intergenic:Lipc-Aqp9 3:intergenic:Lamr1-ps1-Prnp/PRND 4:intergenic:Gm24370-4931417E11Rik 4:intron:Ust 4:intergenic:Gm25715-N6amt1 4:intergenic:Gm6270-Gpbp1 4:intergenic:Slc9a1-Gm13257 4:intergenic:Cdh10-Gm25976 4:intron:Msi2 4:intron:9030617O03Rik 4:intergenic:Gpr141-Gm5446 4:intergenic:Gm24696-Myc 4:intergenic:Gm15031-Gm25915 4:intron:Matn2 4:intergenic:Gm26288-Atp10a 4:intergenic:Mir466f-4-Irx1 4:intergenic:AW146154/Gm20449-Gm6871 4:intergenic:Gm17634-Gm3608 4:intergenic:Gm24295-Mef2c 4:intergenic:Gm11732-Gm11733 4:intergenic:Gm23326-Thy1 4:intergenic:Col18a1-Gm10941 3:intergenic:Banp-Gm22 4:intron:Tenm1 4:intron:Cfdp1 4:intergenic:Gm6421-Hcn1 4:intergenic:Gm25633-Gm8907 4:intergenic:Lmbrd2-Ugt3a1 4:intron:A1cf 4:intron:Gm12158 3:intron:Kcnj5 4:intergenic:Zwint-Pcdh15 4:intergenic:Gm24398-Xpo1 4:intergenic:Creb5-Tril/Gm16499 4:intron:Traf5 4:intergenic:Gm24748-D730003I15Rik 4:intron:Pappa 3:intergenic:Gm11259-Gm11256 4:intergenic:Auts2-Gm25722 4:intron:1700025G04Rik 4:intron:Fryl 4:intergenic:Rxfp2-Fry 4:intron:Slc22a23 4:intron:Gm765 4:intron:Smyd3 4:intron:Car10 4:intron:Mtss1 3:intergenic:Gm5965-Krtap14 2:intron:Ptprk 4:intergenic:Igf2bp2-Gm25810 4:intergenic:Gm3985-Gm6877 4:intron:Camkmt 4:intergenic:5730559C18Rik-Gpr25/Gm26781 4:intergenic:Wipi2-Slc29a4 4:intron:Zhx2 4:intron:Gm8091 4:intron:Zscan22 4:exon:Arhgdia 4:intergenic:Gm14981-Gm26406 4:exon:Ern1 4:intergenic:Gm14515-Gm14513 4:intergenic:Pcsk6-Snrpa1 4:intergenic:Gm23727-Gm20594 4:intergenic:Cyp7a1-4930430E12Rik 3:intergenic:Gm1720-Rpl7a-ps11 4:intergenic:Ufm1-Trpc4 4:intron:Zmat4 4:intergenic:Fam19a1-Fam19a4 3:intergenic:Gm26291-Gm17377 4:intergenic:Hs6st3-Oxgr1 4:intron:Ercc3 4:intron:Prkg1 4:intergenic:Olfr1126-Pramel7 4:intergenic:Gm12763-Rpl23a-ps12 3:intergenic:RP24-225I21.1-Gm26662 4:intergenic:Rasa1-Cox7c 4:intergenic:Plod2-Gm9621 4:intergenic:Gm26915-Nrip1 4:intron:Anxa6 4:intergenic:Zfy2-Gm22119 3:intergenic:Gm26901-Sntg1 4:intergenic:Gm26259-Pitrm1 3:intergenic:Tdrd3-Rps3a2 4:intergenic:Gm9800-Fhit 4:intron:Pitpnm3 4:intron:Clk3 4:intron:Smarca2 4:intergenic:Zfand5-Gda 4:intron:Cdh8 4:intergenic:Glrx3-Gm25798 4:intergenic:Zfp710/AC110903.1-Zfp710 4:intergenic:Rpl21-ps10-Gm25574 4:intergenic:4930550L24Rik-Fgf13 4:intergenic:Cacna2d3-Selk 4:intron:1700047F07Rik 4:intron:Tgfa 4:intergenic:Mageb16-Gm4772 4:intergenic:Grp-Gm25150 4:intergenic:Gm11724/Tmc8-Tmc8 4:intergenic:Rims1-4933415F23Rik 3:intergenic:Bahcc1-Actg1 4:intergenic:Gm25814-Khdrbs2 4:intergenic:Gm25562-AI593442 4:intergenic:Pgrmc2-Gm25487 4:intron:Fyb 4:intergenic:Gm13625-Mettl5 4:intron:4930554H23Rik 4:intergenic:Kif6-Rftn1 3:intron:Bdnf 4:intergenic:Gm11274-Hist1h1b 4:intergenic:Olfm4-Gm9578 4:intergenic:Tmprss2-Ripk4 3:intergenic:Gm5593-Vmn2r60 4:exon:Deaf1 3:intergenic:Prkaca-ps1-Rai2 4:intron:Kcnd2 4:intron:Gm14490 4:intron:Exoc6b 4:intergenic:Ppp1r2-ps9-Gm14519 4:intron:Nck2 4:intergenic:Gm26480-Gm6863 4:intergenic:Gm10192-Gm12696 4:intron:Kalrn 4:intergenic:Gm9956-Gm24285 3:intergenic:Fam84a-Gm9847 4:intergenic:Tgfb2-Rrp15 4:intron:Entpd7 4:intergenic:Mdfic-Tfec 4:intergenic:Sspn-Itpr2 4:intergenic:Gm24017-Fbxw17 4:intergenic:Ctnna3-Gm22829 4:intergenic:Slc16a7-Lrig3 4:intron:Rsbn1l 4:intergenic:Gm13792-Gm13791 4:intergenic:Phactr3-Sycp2 4:intergenic:Bche-Gm6098 4:intergenic:Gm8321-Inhbb 4:intron:A530013C23Rik 4:intron:Mmp16 4:intergenic:Gm25935-Gm22593 4:intron:Kcnma1 3:intergenic:A730090H04Rik/Dlx4-A730090H04Rik 4:intron:Epb4.1l5 4:intron:Cux1 4:intergenic:Gm22805-Gm4791 4:intergenic:Zmynd12-Rimkla/Zmynd12 4:intergenic:Gm10293-Gm22798 4:intron:Cdh10 4:intergenic:Gpatch2-Esrrg 4:intergenic:AC124326.1-Mir802 4:intergenic:Gm24288-Gm17215 4:intergenic:Gm23225-Gm24127 Off-target primers show all... show less...
639 / rev	`TTGAATCCTGTTGGACTTGA TGG` `.................... ...` Cloning / PCR primers	73	83	32	56	60	67	0 - 0 - 5 - 15 - 113 0 - 0 - 0 - 1 - 2 133 off-targets	2:intergenic:Gm5594-Vmn2r-ps59 2:intergenic:Gm2308-Vmn2r60 2:intergenic:Cbln2-Gm5096 3:intergenic:Gm17308-2700069I18Rik 2:intergenic:Gm5593-Vmn2r60 4:intron:Epha7 4:intergenic:Gm5082-Adtrp 4:intron:Lcorl 4:intron:Gmds 3:intergenic:Rnf111-Gm24615 4:intron:Dscam 3:intergenic:Gm24132-Gm25160 4:intron:Gabrb2 4:intergenic:Gm23958-Il1rapl2 3:intergenic:Nmd3-Sptssb 4:intergenic:Gm25051-Gm1818 4:intergenic:Gm22674-Cdyl 3:intergenic:Snd1-Lrrc4 4:intron:Nckap5 3:intron:Plcl2 3:intergenic:Cpne8-Rpl31-ps8 4:intergenic:Olfr1353-Olfr1352 4:intergenic:Cdk19-Slc22a16 4:intergenic:Lpp-A230028O05Rik 4:intergenic:Gm13903-Kcna4 4:intergenic:Pax4-Snd1 4:intergenic:Slc22a23-Pxdc1 4:intergenic:Dzip1-Dnajc3 4:intergenic:Gm12010-Gm12011 4:intergenic:Gm25113-Car10 4:intergenic:Gm24968-Gm22797 3:intergenic:Zkscan2-Gm23788 4:intron:Pcdh9 4:intergenic:Kcna4-Gm9864 4:intergenic:Gm10775-Zfp369 4:intron:Dock5 4:intron:Rabgap1l 4:intergenic:Gm6277/Cables1/Mir1901-Cables1 4:intergenic:Olfr740-Olfr741 4:intergenic:Gm24122-Gm23036 4:intron:Sun1 4:intergenic:Zmat4-A730045E13Rik 4:intergenic:Gm7224-Vmn2r59 4:intron:Prex2 4:intergenic:Gm26072-Gabrg1 4:intergenic:Gm26970-Foxk1 4:intergenic:1700021A07Rik-1700020N01Rik 4:intergenic:Tmem30b-Prkch 4:exon:Gm14463 3:intergenic:Hmgn3-RP23-341H6.1 3:intergenic:Gm12004-4930554G24Rik 4:intron:Tcerg1l 4:intergenic:Pcdh18-Slc7a11 3:intergenic:Gm24555-Pid1 4:intergenic:Pnmal1-Ccdc8 3:intron:Akap3 4:intron:Tacr3 4:intergenic:Gm24519-Mctp1 4:intergenic:Pinc-Gm25329 4:intergenic:Gm24633-Gm25568 4:intergenic:1810019J16Rik-Gm17688/1810019J16Rik 4:intergenic:S100a6-S100a7a 4:intron:Cpxm2 4:intron:Abi3bp 4:intergenic:Etv1-Gm24069 4:intergenic:Gm15729-Nrros 4:intergenic:Mtdh-Laptm4b 4:intron:Pikfyve 4:intergenic:Tmprss15-Rps19-ps12 4:intergenic:Gpr83-Folr4 3:intron:Tmem120b 4:intergenic:Gm13882-D430041D05Rik 4:intergenic:Gm22507-Trhde 4:intergenic:Gm11726-Socs3 4:intergenic:Gm9949-Htr4 4:intergenic:Mir802-Setd4 4:intergenic:D830050J10Rik/Raf1-Gm24008 4:intron:Opcml 4:intron:Phldb2 4:intergenic:Gm26018-BC018507 4:intergenic:Gm1968-4632428C04Rik 3:intergenic:Gm22492-Pdcd6ip 4:intergenic:Gm7290-Gm6471 4:intergenic:Mpped2-Gm22813 4:intergenic:Gm23022-Rab28 4:intergenic:Gm5839-Gm25440 4:intron:Coro7 4:intergenic:4933407K13Rik-Gm7153 4:intergenic:Pacrg-Gm16169 4:intergenic:Foxl1-5033426O07Rik 4:exon:Cldn25 4:intron:Cntn4 4:intron:Lars 4:intergenic:Gm12701-Ror1 4:intergenic:Prickle1-Adamts20 4:intergenic:Maf/Gm15655-Dynlrb2 4:intergenic:Clec14a-Gm17529 4:intron:Snx27 4:intergenic:Gm7293-Arhgap20 4:intergenic:Gm10287-Gm26468 4:intergenic:Slc4a1ap-Mrpl33 4:intergenic:Gm24764-Olfr877 4:intergenic:mmu-mir-6361-2310026I22Rik 4:intergenic:Gm6480-Smarca1 4:intergenic:Prex1-Gm24477 4:intergenic:Olfr742-Olfr743 4:intergenic:Slc10a1-Smoc1 4:intergenic:Gm25133-Gm24770 4:intron:Me3 4:intergenic:Scrt1-Fbxl6 4:intron:Pcyt1b 4:intergenic:Gm13561/Dpp4-Dpp4 4:intergenic:Epha4-Gm26263 4:intergenic:Gm25658-Atp2b1 4:intergenic:Gm25266-Cradd 4:intergenic:Pax4-Snd1 4:intergenic:Wee1-Swap70 4:intergenic:Gm11355-Prl 4:intergenic:Csgalnact1-Gm15654 4:intergenic:Gm24297-Gm22911 4:intron:Clnk 4:intergenic:Ttll7-Gm23131 4:exon:Casp6 2:exon:Srsf6 4:intron:Ctnnd2 4:intron:Nrl 4:intergenic:Prkd1-Gm22088 4:intron:Twf1 4:intergenic:mmu-mir-6412-Ppm1h/mmu-mir-8104 3:intergenic:Gm26146-Gm8226 4:intron:Faxc 4:intron:Ifih1 4:intergenic:Gm5449-Drd1a Off-target primers show all... show less...
95 / fw	`CATGATTGCTCAGGATTGTT GGG` `.................... ...` Inefficient Cloning / PCR primers	72	88	40	53	69	80	0 - 0 - 2 - 22 - 115 0 - 0 - 1 - 3 - 5 139 off-targets	4:intergenic:RP23-442I7.1-Gm17308 4:intergenic:Olfr694-Olfr695 4:intron:Abi3bp 4:intergenic:Gm22957-Lrrc4c 4:intergenic:Gm9726/mmu-mir-8099-2-Gm26055 4:intergenic:1700010H22Rik-1700007G11Rik 4:intergenic:Hapln1-Vcan 4:exon:Mycs 4:intergenic:Dlg5-E330034G19Rik 4:intron:S100z 3:intergenic:Lmo7-Gm22347 4:intergenic:Cd209e-Cd209d 4:intergenic:Ythdc2-Gm26928 4:intergenic:Gm13452-Gm13453 4:intergenic:RP24-456G3.3-RP24-456G3.2 4:intergenic:Gm14838-Gm6206 4:intergenic:Gm12637-Gm22605 4:intron:Spidr 3:intron:Pbx4 4:intergenic:Ccser1-Gm22212 4:intergenic:Gm25656-Cntn6 4:intergenic:Gm10275-Cenpw 3:intron:Pde1b 4:intergenic:Gm22507-Trhde 4:intron:Phlpp1 4:intergenic:Gm6580-Gm1821 4:intergenic:Gm22882-Cxcl12 4:intergenic:Fgf10-Nnt 4:intron:Lactb 4:intron:Pkp4 4:intergenic:Agk-E330009J07Rik 3:intergenic:Fam3c-Gm8927 4:intron:Rora 4:intergenic:Gpr139-Gm23321 4:intergenic:Gm5127-Gm22102 3:intergenic:Dmtf1-Gm24666 4:intergenic:Gm12506-Gm12519 4:intron:Grik5 4:intergenic:Ran-Gpr133 3:intron:Gabrg3 4:intron:Grid2 2:intergenic:Gm7224-Vmn2r59 3:intergenic:Gm2308-Vmn2r60 3:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Fbxo15-Gm25453 4:intron:Rgs7 4:intergenic:Ntm-Gm15520 4:intron:Ube2q1 4:intergenic:Slco6c1-D1Ertd622e 4:intron:Mlip 3:intron:Arid1a 4:intergenic:Plekhg1-Gm16149 3:intergenic:Gm9431-Gm14865 4:intergenic:Gm24919-Efcab2 4:intergenic:Gm14258-9430093N23Rik 4:intergenic:Gm23514-5730522E02Rik 4:intron:Nbas 3:intron:Zmat4 4:intergenic:Gm9931-Gm22966 4:intergenic:Ythdf3-Gm26485 4:intron:Bai3 4:intergenic:Gm22135-Gm6776 4:intergenic:Gm26090-Lef1 4:intron:Plcb4 4:intergenic:AC116451.1-Rpl13a-ps1 4:intron:Slc19a1 4:intergenic:Enpp6-4930579M01Rik 4:intergenic:Gm25460-Gm5263 4:intron:Zc2hc1b 4:intron:Aip 3:intergenic:Gm8312-1700036O09Rik 4:intergenic:Olig3-Ifngr1 4:intergenic:B3galtl-Rxfp2 4:intron:Wdfy4 3:intergenic:Gm13982-Gm13985 4:intron:Col16a1 4:intergenic:9130204L05Rik-Gm23697 4:intergenic:Gm23181-Fam174a 4:intron:Scn8a 3:intergenic:Gm26651-Diras2 4:intergenic:Gm16685-Gm24614 3:intergenic:Gm26468-Rpsa-ps10 4:intergenic:Rgs6-Dpf3 3:intron:Hspg2 4:intron:Arid1a 4:intron:Gm15397 3:intron:Arhgef6 4:intergenic:A430057M04Rik-Zfp503 4:intron:Tbrg1 4:intron:Dmd 3:intron:Wdr7 4:intergenic:Gm25258-Hao1 4:intergenic:Clcnkb-Hspb7 3:intron:Srrm4 3:intergenic:Gm6576-Gm23903 4:intergenic:n-R5s175-Gm2301 4:intergenic:Mbnl2-Gm26679 4:intergenic:En1-B230209K01Rik 4:intergenic:Gm17054-n-R5s37 4:intergenic:Ube2o-Gm11742 4:intron:Gm14010 4:intergenic:Gm14670-Gm14675 4:intergenic:Zfp516-Gm24720 4:intron:Zscan5b 3:intergenic:Gm24948-Nova1 4:intergenic:Reep5-Pkd2l2 4:intergenic:Tcerg1l-Mapk1ip1 4:intron:Enkur 4:intergenic:AC112986.1-Tsn 4:intergenic:Gm6584-Gm15861 4:intergenic:Gm17538-Gm5229 4:intergenic:Gm13393-Gm13396 4:intergenic:Gm23733-6530403H02Rik 4:intergenic:Gm10710-Gm22342 4:intergenic:Rbbp8-Gm6277 4:intron:Tpd52 4:intron:Kcnh8 4:intergenic:Gm26861-Gm25496 4:exon:Runx3 4:intron:Disc1 2:intergenic:Gm7847-Gm23674 3:intergenic:Gm16998-Gm26177 4:intron:Kcnma1 4:intergenic:Hif1an-Gm26644 4:intron:Cdyl 4:intergenic:Ruvbl1-Sec61a1 4:intergenic:Gm831-Gm12962 4:intergenic:Fbxo15-Gm25453 4:intron:Rbm26 4:intergenic:Gm3810-Gm12079 4:exon:Arhgef26 4:intron:Ankk1 4:intergenic:Il17rd-Arhgef3 4:intergenic:Gm25404-Nbea 4:intron:Tesc 4:intergenic:8030451A03Rik-Tnc 4:intergenic:Cacng3-Rbbp6 4:intergenic:Ranbp6-Il33 4:intron:Tmem144 Off-target primers show all... show less...
397 / rev	`GTGGGCACATATGTTACATA TGG` `.................... ...` Enzymes: NdeI, NlaIV, NdeII, BspPI, BamHI, MflI Cloning / PCR primers	72	84	44	49	56	75	0 - 0 - 2 - 10 - 238 0 - 0 - 0 - 1 - 4 250 off-targets	4:intergenic:Gm12789-Gm12799 4:intergenic:Gm3944-Gm5784 3:intron:Usp18 3:intergenic:Gm15983-n-R5s30 4:intergenic:Fam126a-Klhl7 3:intron:Pcdh15 4:intergenic:Gm25460-Gm5263 4:intergenic:Gm26970-Foxk1 4:intergenic:Gm25672-Barhl2 4:intergenic:U7-Gm10192 4:intron:Pitpnb 4:intergenic:Gm14601/Gm15482-Gm14644 4:exon:Larp4b 4:intron:Gm15854 4:intergenic:Cxcl14-Gm24716 4:intergenic:Gm21722-Gm21282 4:intergenic:9330101J02Rik-Gm4459 2:intron:Pik3ap1 4:intron:Capn3 4:intergenic:Gm6580-Gm1821 4:intergenic:Gm25925-Hpcal1 3:intergenic:Gm11623-Gm11621 4:intron:Gprin3 4:intergenic:Gm24997-AC166813.1 4:intergenic:Gm25519-Gm5548 3:intron:Zan 4:intron:Dnah3 3:intergenic:Kctd8-Kctd8/Gm24368 4:intron:N6amt1 3:intron:Psmd1 4:intergenic:Brd7-Nkd1 4:intergenic:C630043F03Rik-Gm11235 4:intergenic:Gm21923-Gm20880 4:intergenic:Gm21209-Gm21316 4:intergenic:Gm20895-Gm20892 4:intergenic:Gm20932-Gm21524 4:intergenic:Gm20865-Gm21065 4:intergenic:Gm21913-Gm22832 4:intergenic:Gm20810-Gm21853 4:intergenic:Gm21894-Gm23052 4:intergenic:Gm20907-Gm20921 4:intergenic:Gm21427-Gm21890 4:intergenic:Gm21757-Gm20846 4:intergenic:Gm21111-Gm21875 4:intergenic:Gm21841-Gm20842 4:intergenic:Gm21855-Gm20902 4:intergenic:Gm21518-Gm20823 4:intergenic:Gm20901-Gm21865 4:intergenic:Gm21825-Gm21333 4:intergenic:Gm21735-Gm21732 4:intergenic:Gm21767-Gm20822 4:intergenic:Gm20893-Gm21642 4:intergenic:Gm21654-Gm20882 4:intergenic:Gm20810-Gm21853 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm20935-Gm20937 4:intergenic:Gm20798-Gm20896 4:intergenic:Gm21198-Gm20808 4:intergenic:Gm20881-Gm20889 4:intergenic:Gm21729-Gm21774 4:intergenic:Gm20913-Gm21868 4:intergenic:Gm20738-Gm21924 4:intergenic:Ssty2-Gm21114 4:intergenic:Gm21477-Gm21813 4:intergenic:Gm21766-Gm20800 4:intergenic:Gm21902-Gm21285 4:intergenic:Gm21739-Gm21856 4:intergenic:Gm21788-Gm20826 4:intergenic:Gm21117-Gm20884 4:intergenic:Gm21892-Gm23770 4:intergenic:Gm21766-Gm20800 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm20840-Gm21845 4:intergenic:Gm21746-Gm20873 4:intergenic:Gm21829-Gm20912 4:intergenic:Gm20901-Gm21865 4:intergenic:Gm20888-Gm20886 4:intergenic:Gm21808-Gm20866 4:intergenic:Gm21076-Gm25359 4:intergenic:Gm21805-Gm21241 4:intergenic:Gm20931-Gm21733 4:intergenic:Gm21749-Gm21302 4:intergenic:Gm20837-Gm21860 4:intergenic:Gm20923-Gm21249 4:intergenic:Gm20815-Gm21292 4:intergenic:Gm20884-Gm21387 4:intergenic:Gm21160-Gm20932 4:intergenic:Gm21476-Gm21506 4:intergenic:Gm20843-Gm20844 4:intergenic:Gm20736-Gm20936 4:intergenic:Gm20898-Gm21799 4:intergenic:Gm21804-Gm20810 4:intergenic:Gm21171-Gm20924 4:intergenic:Gm21095-Gm21380 4:intergenic:AC201846.1-Gm20910 4:intergenic:Gm21877-Gm21530 4:intergenic:Gm21333-Gm21344 4:intergenic:Gm21824-Gm21879 4:intergenic:Gm21895-Gm20933 4:intergenic:Gm21822-Gm21763 4:intergenic:Gm21634-Gm21654 4:intergenic:Gm21906-Gm21920 4:intergenic:Gm21874-Gm20821 4:intergenic:Gm21813-Gm21777 4:intergenic:Gm20926-Gm21768 4:intergenic:Gm21901-Gm21864 4:intergenic:Gm20892-Gm21771 4:intergenic:Gm20914-Gm21778 4:intergenic:Gm21661-Gm21907 4:intergenic:Gm20865-Gm21065 4:intergenic:Gm21777-Gm21792 4:intergenic:Gm21776-Gm21155 4:intergenic:Gm21919-Gm21790 4:intergenic:Gm20902-Gm21715 4:intergenic:Gm21257-Gm20791 4:intergenic:Gm21396-Gm21245 4:intergenic:Gm20806-Gm20919 4:intergenic:Gm21258-Gm21268 4:intergenic:Gm21506-Gm20891 4:intergenic:Gm20916-Gm21823 4:intergenic:Gm21573-Gm20803 4:intergenic:Gm21734-Gm24828 4:intergenic:Gm21862-Gm23863 4:intergenic:Gm21815-Gm20913 4:intergenic:Gm21835-Gm21878 4:intergenic:Gm20813-Gm21922 4:intergenic:Gm21720-Gm21838 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm20799-Gm21795 4:intergenic:Gm21778-Gm21746 4:intergenic:Gm20816-Gm20818 4:intergenic:Gm21281-Gm21308 4:intergenic:Gm20853-Gm20855 4:intergenic:Gm21914-Gm21852 4:intergenic:Gm21858-Gm21340 4:intergenic:Gm21151-Gm21160 4:intergenic:Gm21760-Gm21257 4:intergenic:Gm21889-Gm20852 4:intergenic:Gm21412-Gm21773 4:intergenic:Gm21198-Gm20808 4:intergenic:Gm21242-Gm21248 4:intergenic:Gm21443-Gm21755 4:intergenic:Gm21163-Gm21180 4:intergenic:Gm21201-Gm20853 4:intergenic:Gm21617-Gm21634 4:intergenic:Gm20809-Gm21921 4:intergenic:Gm21065-Gm21871 4:intergenic:Gm20826-Gm20825 4:intergenic:Gm21573-Gm20803 4:intergenic:Stmn4-Gm23899 4:intergenic:Gm11235-Aldoart1 4:intergenic:Nckipsd-Celsr3 4:intron:Ptprm 4:intergenic:Pwwp2b-Inpp5a 4:intergenic:NAMA_2-Gm22670 4:intergenic:Gm10654-Gm17576 4:intergenic:Clic5-Runx2 3:intron:L3mbtl4 4:exon:Cnksr3 4:intron:Man1c1 4:intron:Tmem163 4:intron:Dlgap2 4:intergenic:Gm26256-n-R5s61 4:intergenic:Art4-Mgp 4:intergenic:5033403F01Rik-Gm23972 4:intron:Lingo2 4:intron:Cep85 3:intron:Disp1 4:intron:Gm13974 4:intron:Atmin 4:intron:BC051142 4:intergenic:Snap91-Ripply2 4:intergenic:Gm10549-2410004N09Rik 4:intron:Cask 4:intergenic:Mrpl3-Nudt16 4:intron:Nap1l4 4:intergenic:Tmem232-Vapa 4:intergenic:Mepe-Spp1 4:intron:Osbpl11 4:intergenic:mmu-mir-6343-Gm25974 4:intron:Kcnj9 4:intergenic:Gm10654-Gm17576 4:intergenic:Gm10654-Gm17576 4:intergenic:Nap1l2-Gm9050 4:intergenic:A830019P07Rik-Htr7 4:intron:Ltbp1 4:intergenic:Dhx35-Gm25090 4:intergenic:1700034G24Rik-Myo18b 2:intergenic:Ntng2-6530402F18Rik/Ntng2 4:intergenic:4930595D18Rik-Traf3 4:intron:Ska3 4:intergenic:Barhl1-Gm13394 4:intron:Hydin 4:intergenic:Ahi1-Myb 4:intergenic:Gm25562-AI593442 4:intergenic:Gm7719-Gm24377 3:intron:Ctnna3 4:intergenic:Clcn5-Usp27x 4:intron:Clcn3 4:intron:Camk2b 4:intron:Tfec 4:intergenic:Gm10222-Lmbrd1 4:intergenic:Gm15221-E130120K24Rik 4:intergenic:Smoc2-Thbs2 4:intergenic:Gm12753-Gm12751 4:intergenic:Gm25094-Gm22312 4:intergenic:Gm5619-9330154J02Rik 4:intron:5730522E02Rik 4:intron:Agrn 4:intron:Cep89 4:intron:Hat1 4:intergenic:Nrg2-Pura 4:intergenic:Gm25564-Dcdc2c 4:intergenic:Btbd11-Pwp1 4:intergenic:Pla2g4e-Pla2g4e/Gm13997 4:intron:Skint9 4:intergenic:Gm26022-Chl1 4:intergenic:Gm24132-Gm25160 4:intron:2210408I21Rik 4:intergenic:Gm14254-Tox2 4:exon:Zfp384 4:intergenic:Tceanc2/Gm12802-Tceanc2 4:exon:Tmem26 4:intergenic:Jam3-Igsf9b 4:intergenic:Syn3-Gm6729 4:intergenic:Igbp1b-Gm25434 4:intergenic:Zcchc16-Gm26024 4:intergenic:Ntf3-Kcna5 4:intergenic:Dock9-Ubac2 4:exon:Bbs4 4:intergenic:Gm9843-Usp25 4:intron:Mme 4:intergenic:Fam217a-4933417A18Rik 4:intergenic:Gm21788-Gm20826 4:intergenic:mmu-mir-7671-Irf1 4:intergenic:Gm22582-Nedd1 4:intron:Slit3 4:intergenic:Gm20830-Gm21244 4:intron:Pik3c2a 4:intergenic:Irx2-Irx4 4:intergenic:Fshr-Gm10184 4:intron:Ate1 4:intron:Ank3 4:intergenic:Gm5174-1810014B01Rik 4:intergenic:mmu-mir-6365-Gm25276 4:exon:Gm11405 4:intergenic:BC039966-A430005L14Rik 4:intron:Sulf1 4:intergenic:Olfr202-Olfr203 Off-target primers show all... show less...
100 / fw	`TTGCTCAGGATTGTTGGGAA GGG` `.................... ...` Cloning / PCR primers	71	83	62	79	66	84	0 - 0 - 3 - 24 - 163 0 - 0 - 0 - 2 - 3 190 off-targets	3:intergenic:Sv2c-Gm25213 4:intron:Med13l 4:intergenic:Gm24441-Gm24870 4:intron:Wdr72 3:intergenic:Gm5555-Gpr125 4:intergenic:Gm5828-Jph1 4:intergenic:Gm4409/Lrrtm4-Lrrtm4 4:intron:Tox3 4:intergenic:Gm24635-Arrdc3 4:intron:Ccdc162 4:intergenic:Gm15389-Gm24267 4:intergenic:C330046G13Rik-Tcp11l2 3:intergenic:1700001G01Rik-Gm26235 4:intergenic:Gm22727-Gm4258 4:intergenic:Gm25722-Gm10051 4:intergenic:Gm24456-Gm15400 4:intron:Chchd3 4:intron:Larp1 4:intron:Fam13c 3:intergenic:Calcoco2-Ttll6 4:intergenic:Muc6-Muc2 3:intron:Cntnap5a 4:intron:Cadps 4:exon:Xpo6 4:intron:Pak3 4:intergenic:Gm6274-Slc25a43 4:intergenic:Mmd-Hlf 3:intergenic:A630012P03Rik-Gpc3 4:intergenic:Gm23553-Larp4b 4:intergenic:4930562F07Rik/4930523C07Rik-Tnn 4:intron:Knstrn 4:intergenic:Atp2c1-Gm5621 4:intergenic:Bmper-Npsr1 4:intergenic:Mbd2-Dcc 4:intergenic:Gm11189-Hspa4 4:intergenic:Gm13294-Prkcq 4:intron:Phex 4:intron:Vsig10 4:intergenic:Gm11189-Hspa4 4:intergenic:Ins1-Xpnpep1 4:intergenic:Gm26617/Zxda-Gm371 4:intergenic:Adcy5-Gm25179 4:intergenic:Gm5402-Zxdb 4:intron:Adamts9 2:intron:Atp9b 4:intergenic:Gm15636-Eml1 3:exon:Bbx 4:intergenic:Gm10827-Nhsl1 4:intergenic:Capza3-Gm24174 4:intergenic:Kazald1-Tlx1 4:exon:Cul4a 3:intergenic:Gm7224-Vmn2r59 4:intron:Il22ra1 4:intergenic:Hdac11-Gm14573 4:exon:Elavl4 4:intergenic:Gm27023-Gm25800 4:intron:Gm15155 4:intergenic:Gm24341-Cnr1 4:intron:Kcnc1 4:intergenic:Gm4784-Gm4991 4:intron:Fam65c 4:intergenic:Slamf6-Vangl2 4:intron:Brms1 4:intron:Apoo 3:intron:Epha6 4:intergenic:Fbxl17-A930002H24Rik 4:intergenic:Gm11787-Mir3471-2 4:exon:Cyb5r1 4:intergenic:Dscam-Bace2 4:exon:4930503H13Rik 4:intergenic:Nos1ap-Olfml2b 4:exon:Nrcam 4:intergenic:Gm25985-Gm6404 4:intergenic:Mfsd4-Mfsd4/Gm26892 4:intron:AI607873 4:intergenic:Klrb1c-Klrb1b 4:intergenic:Gm26245-Gm26078 4:intergenic:Elk3-mmu-mir-7688 4:intron:Slc36a3 4:intergenic:Gfra2-Gm9192 4:intron:Gm872 4:intron:Tpd52l2 4:intergenic:Itgb1-Gm26100 4:intergenic:Eif4g2-Galnt18 4:intergenic:Gm22597-Gm24514 4:intergenic:Gm25005-Gm22819 4:exon:Dnhd1 3:intron:Cacna1d 4:intergenic:Gm5257-mmu-mir-6343 4:intergenic:Gm25278-Csmd1 4:intron:Armc4 4:intron:Fancl 4:intergenic:SOX2OT_exon2/Sox2ot-Sox2ot 3:exon:Nrp1 4:intron:Plekhg6 4:intergenic:Gm23534-Gm22727 4:intron:Ppfibp2 4:intron:Tspan9 4:intergenic:Gm24750-Gm24590 4:intergenic:Gm8840-Ikzf2 3:intron:Zic4 4:intergenic:Gm23423-Gm26548 4:intergenic:Unc5d-4933433F19Rik 4:intergenic:D5Ertd579e/Gm25767-D5Ertd579e 4:intergenic:Robo1-Gm25699 4:intergenic:Gm9915-2610017I09Rik 4:intergenic:Fndc3a-Cysltr2 4:intergenic:Mir690-Mb21d2 4:intron:Cd101 4:intergenic:Gm5403-Mdm4-ps 4:intergenic:Gm20873-Gm21719 3:intergenic:RP24-86O15.2-Gm10698 4:intron:2810032G03Rik 4:intergenic:Gm26468-Rpsa-ps10 4:intergenic:Gm26373-Kcnd2 4:intergenic:Irf8-Gm26878 4:intron:Pard3b 3:intron:Akap4 4:intergenic:Jag1-Gm14055 4:intergenic:Smad1-Gm8079 4:intron:Ptprn2 4:intron:Tmtc2 4:intergenic:Pdcd1-Gm23389 3:intergenic:Got1-Gm20467 3:intron:Sema6d 4:intron:Nipsnap1 4:intergenic:Gm23315-Rprm 4:intergenic:Gm24063-Rps15a 3:intron:Crabp1 2:intergenic:Gm8730-Cdh5 3:intergenic:Gm9001-Gm22283 4:intron:Ntrk2 4:exon:Slc16a6 4:intron:Nrg2 3:intron:Dcaf5 4:intergenic:4930463O16Rik-Ckap4 4:intergenic:Irf2bp2-Tomm20 4:intergenic:D13Ertd608e-Tcstv1 3:intergenic:Clcnkb-Hspb7 4:intergenic:Gm26535-Gm24710 4:intron:Tgfb2 3:intergenic:Pax7-Gm1667 4:intergenic:Gzme-Gzmd 4:intergenic:Gzmd-Gzmg 4:intergenic:Mcm5-Rasd2 3:intergenic:Gm12160-Gm12161 4:intergenic:Pik3r1-Gm25808 4:intron:Kirrel3 3:intergenic:Gm5594-Vmn2r-ps59 4:intron:Gm5087 4:intron:Dcun1d3 4:intergenic:Mir3962-Gm23885 4:intergenic:Gm20416-Yap1 4:intron:Zfp748 4:intron:Irf2 4:intergenic:Glb1l3-Gm1110 4:intergenic:Rassf6-Cxcl5 4:intergenic:Tro-Gm15132 2:intergenic:Lmo4-Hs2st1 3:intergenic:Gm25200-Cdh13 4:intergenic:4930555K19Rik-Oxr1 4:intergenic:Cacna1e-Gm9530 4:intron:Sez6l 4:intergenic:Gm13518-mmu-mir-195b 4:intergenic:Sphkap-Gm25754 4:intergenic:Cadm2-Gm24681 4:intergenic:Olfr591-Olfr592 4:intergenic:Cdh10-Gm25976 4:intergenic:Tmem126b-Dlg2 4:intergenic:Gm25723-Gm22459 4:intron:Csnk2a2 4:intron:Ptprt 4:intergenic:Ugt2b5-Ugt2b37 4:intron:Aff1 4:intergenic:Gm2301-Gm16063 4:intergenic:Gm26727/Gm21830-Xirp2 4:intron:Zeb1 4:intergenic:Gm17315-Rmdn2 4:intron:Phlpp1 4:intergenic:Gm13786-Gm13785 4:intron:Camta1 4:intron:Fmo5 4:intergenic:Gm23186-Arid1b 4:intergenic:Gm10384-Zfp706 4:intron:Kalrn 4:exon:Ppat 4:intron:Vapa 4:intergenic:Adamts6-Cwc27 4:intergenic:Tacr3-Gm24048 4:intergenic:Gm10710-Gm22342 Off-target primers show all... show less...
465 / rev	`CATCAACCTGTAAACCATGA AGG` `.................... ...` Enzymes: TspDTI, NlaIII Cloning / PCR primers	70	85	63	36	53	79	0 - 0 - 3 - 12 - 145 0 - 0 - 0 - 1 - 8 160 off-targets	2:intron:Vmn2r57/Gm20449 4:intergenic:Tnip3-Ndnf 4:intron:Epha5 4:intergenic:Ebf1-Gm12159 3:intergenic:Gm13404-C230014O12Rik 4:intergenic:Gm13018-Prdm2 4:intron:Pde10a 4:intron:Sash3 4:intergenic:Fgf10-Nnt 4:intergenic:Gm26235-Gm25392 4:intron:Yeats2 4:intergenic:Snx7-Gm26871 4:intergenic:Unc13c-Gm25105 4:intron:Zpld1 4:intron:Foxo3 4:intergenic:Satb1-RP24-465O15.1 4:intergenic:Cadm2-Gm24681 4:intergenic:Rpl15-ps2-Tcf12 4:intron:Mctp2 3:intergenic:Gm5594-Vmn2r-ps59 4:intron:Alox5 4:intron:Tbc1d1 4:intron:Nkiras2 4:intergenic:Cyp39a1-Rcan2 4:intron:Cyp4f15 4:intergenic:Klhl1-Gm24043 4:intron:Epc2 4:intergenic:Gm4994-Gm7058 4:exon:Fggy 4:intron:Tlr4 4:intergenic:Gm6098-Zbbx 3:intron:Gm11884 4:intergenic:Gm25306-Gm26761 4:intergenic:8430403D17Rik-Mir5110 3:intron:Zfp940 4:intergenic:Gm24374-Sash1 4:intron:Snta1 4:intergenic:Gm23022-Rab28 4:intron:Fkbp1a 4:intergenic:mmu-mir-6393-Zdhhc25 4:intergenic:Xkr4-Gm1992 3:intron:Trpc4 4:intron:Notch2 4:intergenic:Gm5530-mmu-mir-6344 4:intergenic:Gm26489-1700028P15Rik 4:intron:Agxt 4:intergenic:Gm26349-Mrps30 4:exon:B3galt5 3:intergenic:Gm17333-Gm25025 4:intergenic:Cacna2d3-Selk 4:intron:Masp1 4:intergenic:Slc25a51/Gm25692-Slc25a51 4:intergenic:AC175117.1-Hnf4g 3:intergenic:Trim29-Pvrl1 4:intergenic:Tbc1d1-Gm25306 4:intron:Tmem178 4:intergenic:Gm13851-9430029A11Rik 3:exon:Slc22a1 4:intergenic:Gm14043-Stk35 4:intergenic:Gm22364-Gm14569 4:intron:Lrp2 4:intergenic:Gm13018-Prdm2 4:intergenic:Gm12161-Gm12162 4:intron:Wrn 4:intron:Rarres1 4:intergenic:Olfr457-Olfr456 4:intergenic:Olfr458-Olfr457 4:intergenic:Il1rl2-Il1rl1 4:intergenic:Slc30a5-Gm10257 4:intron:Gnat2 4:intron:Arhgef3 4:intergenic:Smad7-Ctif 4:intergenic:Zcchc6-Gas1 4:intergenic:Gm17100-Gm16041 4:intron:Slc15a4 4:intron:Wdr93 4:intron:Nckap5 4:intergenic:Gm23387-Gmpr 3:exon:Pabpc1 3:intergenic:Gm23013-Ppp1r3a 4:intron:Gm14055 4:intron:Lgr6 4:intergenic:Gm25813-Gm23666 4:intergenic:Gm25875-Gm23835 4:intron:Setdb2 4:intron:Slc44a4 4:intergenic:Gm17131-Tmem132d 4:intergenic:Rab27b-Dynap 4:intron:Ubxn4 4:intron:Pias1 4:intron:Nav2 4:intergenic:Hmga2-Gm24298 4:exon:Ftcd 4:intron:Zmat4 4:intergenic:Gm4804-Trib2 4:exon:Bcl9l 4:intergenic:Gm26190-B3gat1 4:intron:Epb4.1l4b 4:intron:Scgn 4:intron:Zc3h3 4:intergenic:5031434O11Rik-Mgst2 4:intron:Nav2 4:intergenic:Gm22035-Slc35b3 4:intergenic:Has2os-Gm26178 4:intergenic:Gm17571-Maml2/Gm22643 4:exon:Obscn 4:intergenic:Smoc2-Thbs2 4:intergenic:Hdac8-Gm14858 4:intergenic:Irx2-Irx4 4:intergenic:Ptf1aos-Ptf1a/Ptf1aos 4:intron:Impg1 4:intergenic:Rassf9-Alx1 4:intergenic:Eid2-Eid2b 4:intergenic:Eid2-Eid2b 4:intergenic:Eid2-Eid2b 4:intergenic:Eid2-Eid2b 4:intergenic:Gm9958-Alb 4:exon:Tktl1 4:intergenic:Gm25633-Gm8907 4:intergenic:Mbd5-Gm13509 4:intergenic:D630014O11Rik-Galnt14 4:intergenic:Gm12600-Gm23154 4:intergenic:Arhgef15-Slc25a35 4:intergenic:4930596M17Rik-Lyzl4 4:intron:Rapgef1 4:intergenic:Gm15323-Ankrd55 4:intron:Ifitm10 4:intergenic:Gm24680-4921530L21Rik 2:intergenic:Gm12801-Gm12796 4:intergenic:Gm24070-Gm24994 4:intron:Runx1 3:exon:Gng2 4:intergenic:Gm13389-Gm10855 3:intergenic:Ddx17-Dmc1 4:intergenic:Gm6317-Gm25383 4:intergenic:Ctbp2-Ctbp2/Fgfr2 4:intergenic:Psen2-Gm5069 2:exon:Slc35f2 4:intergenic:Gm5885-Gm23375 4:intergenic:Gm23186-Arid1b 4:intron:Nrxn3 4:exon:Lbp 4:intergenic:Gm22507-Trhde 4:intergenic:Impad1-Gm11779 4:intergenic:Ralyl-Gm22026 4:intergenic:Gm12830-9130410C08Rik 4:intergenic:Rnf144b-4931429P17Rik 4:intergenic:Dppa4-Dppa2 4:intergenic:St3gal1-Zfat 4:intergenic:Gm26257-Gp49a/Lilrb4 4:intergenic:Immp2l-Gm25497 4:intron:Magea10 4:intergenic:Gm10051-Gm15627 4:intron:Glt1d1 4:exon:Slc28a2 4:exon:Gm14085 4:intergenic:Tspy-ps-Uty 4:intergenic:Pcbp3-Gm10787 4:intergenic:Gm10123-Spsb4 4:intergenic:Gm15514-Gm24932 Off-target primers show all... show less...
648 / rev	`AGACACAATTTGAATCCTGT TGG` `.................... ...` Enzymes: LpnPI Cloning / PCR primers	70	85	56	8	54	79	0 - 0 - 2 - 27 - 140 0 - 0 - 0 - 3 - 5 169 off-targets	4:intergenic:Tspan8-Ptprr 4:intergenic:Cast-Hnrnpa1l2-ps 4:intron:Kcnma1 3:intron:Dpy19l1 4:intergenic:Gm21824-Gm21879 3:intergenic:Gm11746-Slc24a3 3:intron:Tec 3:intron:Tmem116 4:intergenic:Cep192-Ldlrad4 3:intergenic:Gm7931-Slit2 3:intergenic:n-R5s30-Rbfox1 4:intergenic:Gm13582-Gm13579 4:intergenic:Gm26844-Gm4812 4:intergenic:Gm15210-Gm5742 4:intergenic:Gm21848-Gm20736 4:intergenic:Gm21840-Gm21258 4:intergenic:Inpp4b-4930579O11Rik 4:intergenic:Gm5607-D630011A20Rik 4:intergenic:Cstf2t-Prkg1 4:intergenic:Gm11791-Gm11793 4:exon:Hnrnpf 4:intergenic:Gm23503-Fign 4:intergenic:Gm6430-Slco6c1 4:intergenic:Invs-Gm12435 4:intergenic:Gm6491-Gm14911 4:intron:Adgb 4:intergenic:1700001K19Rik-Hsp90aa1 4:intergenic:Cfh-Kcnt2 4:intergenic:Mtnr1a-F11 4:intergenic:Gm833-Gm25779 4:intergenic:Cdh18-Gm24656 3:intron:Btbd7 4:intergenic:Gm24487-Ctnnd2 4:intergenic:Gm15728-Ksr2 3:intergenic:Gm22882-Cxcl12 4:intergenic:Svil-Gm26682 4:intergenic:Gm24469-Mcph1 4:intron:Spp2 4:intergenic:D3Ertd751e-Gm25714 4:intron:Trpm3 3:intergenic:Gm14226-a 4:intergenic:RP24-326O4.1-Mlip 4:intron:MDGA2/Mdga2 4:intergenic:Herc3-Fam13a 4:intergenic:4930547M16Rik-2010001E11Rik 3:intergenic:Olfr1373-Olfr1372-ps1 3:intergenic:Gm23950-Gm11218 4:intergenic:Gm23315-Rprm 4:intergenic:Gm7742-Vmn1r-ps147 3:intron:Hhat 4:intergenic:Ces2d-ps-Ces2e 4:intergenic:Gm22825-Zfhx3 4:intergenic:Xrcc6bp1-Ctdsp2 4:intergenic:Gm21726-Atp6ap1l 4:exon:9530036O11Rik 4:intergenic:Gm5912-Gins3 4:intron:Dpp6 4:intergenic:Lypd3-Tex101 4:intergenic:Mrpl1-Fras1 4:intergenic:Gm23591-Speer2 4:intergenic:Gm11796-Fam110b 3:intergenic:Zfp316-Zfp853 3:intron:Ube4b 4:intergenic:Gm22198-Gm26975 4:intergenic:Gm15577-Negr1 4:intron:Snta1 4:intron:Gpr126 4:intergenic:Rarb-Thrb 2:intron:Gm973 4:intron:Fbxo36 4:intron:Myo1e 4:intergenic:Olfr1383-Olfr1382 4:intron:Vsig1 4:intergenic:Cldn23-Gm22030 4:intron:Sbf2 4:intergenic:Gm10292-Schip1 4:intergenic:D030068K23Rik-Gm23163 4:intron:Cdh13 4:intergenic:Eya4-Rps12 4:intron:Ppp1r9a 4:intergenic:Mapk1ip1l-Lgals3 4:intergenic:Gm25306-Gm26761 4:intergenic:Tmprss4-BC049352 4:intergenic:Olfr1006-Olfr1008 4:intergenic:Foxd1-Gm2445 4:intron:Immp2l 4:intron:Nkain2 4:intergenic:4930552P12Rik-Tcf7l2 4:intergenic:Gm13313-Gm13367 4:intergenic:1810046K07Rik-Gm7293 4:intergenic:Gm6369-Armc1 4:intergenic:Gm6369-Armc1 4:intergenic:Crry-ps-AC161214.1 4:intergenic:Psg26-Psg25 4:intron:Slc12a8 4:exon:Mtfr2 4:intergenic:Gm22797-Robo1 4:intergenic:Myo16-Rps16-ps3 4:intergenic:Gm20834-Gm20737 4:intergenic:Gm23558-Galc 4:intergenic:Gm17177-Speer6-ps1 4:intergenic:Gm22084-Gm24502 4:intron:Slc9a9 3:intergenic:Gm11235-Aldoart1 4:intron:Rps6ka5 4:intergenic:Klhl25-Gm5597 4:intergenic:Hmx1-Cpz 3:intergenic:Gm13976-3110099E03Rik 3:intron:Ssbp3 4:intergenic:Pced1b-Rpap3 4:intergenic:Gm10398-Gm26660 4:intergenic:mmu-mir-6366-Gm7873 4:intron:Myo3a 4:intergenic:Gm25261-Gm831 4:intergenic:Slco1c1-Slco1b2 4:intron:Dlgap1 2:intron:Egfem1 4:intergenic:Pcca-Ggact 4:intron:Itpr2 4:intron:Arid1b 3:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:Gm14765-Tab3 4:intergenic:Mir3964-Gm23033 4:intergenic:Cntn5-Gm25365 4:intron:Ptbp2 4:intergenic:Fam19a1-Fam19a4 4:intergenic:HYMAI/Plagl1-Plagl1 4:intergenic:Gm23465-Vtcn1 4:intergenic:4921524L21Rik-Rab18 4:intergenic:Akt3-Gm26801 4:intron:4930432B10Rik 4:intergenic:A830010M20Rik/1700028K03Rik-1700028K03Rik 3:intron:Nrxn3 4:intergenic:4631405K08Rik-Plxna2 3:intergenic:Ston2-Gm8378 3:intron:Stat3 3:intergenic:4930527B05Rik-1700071K01Rik 3:intron:Mrpl27 4:intergenic:BC052040-Meis2 4:intergenic:Gm26430-Gm23895 4:intron:Kalrn 4:intron:C130046K22Rik 4:intron:Myo16 4:intergenic:PCGEM1-Gm25843 3:intergenic:Gm16685-Gm24614 4:intergenic:Plxna1-Chchd6 3:intron:Ptprz1 4:intron:Synpo 4:intergenic:Gm22785-Gm8410 3:intergenic:Gm5866-8030423F21Rik 3:intron:Ddx26b 4:intergenic:Gm16477-Gpr26 4:intergenic:Gm6215-Gm5128 4:intergenic:Gm15023-Tceal6 4:intergenic:Atrx-Magt1 4:intergenic:Golgb1-Hcls1 4:intergenic:Gm10804-Gm10803 4:intron:Tenm2 4:intron:Gm11751 4:intergenic:Gm22970-Gm5958 4:intergenic:Gm12851-Usp1 4:intron:Pou6f2 4:intergenic:Tigit-Drd3 4:intergenic:Suclg2-Fam19a1 4:intron:Ghr 4:intron:Zfp938 4:intergenic:Kctd16-Rps19-ps13 4:intergenic:Malt1-Zfp532 4:intergenic:Gm23677-Maf Off-target primers show all... show less...
786 / fw	`CATTGGGATGTACAGATTTA TGG` `.................... ...` Inefficient Cloning / PCR primers	70	82	24	36	66	81	0 - 0 - 3 - 21 - 163 0 - 0 - 0 - 0 - 3 187 off-targets	4:intergenic:Lcorl-Gm7931 3:intergenic:Gm24602-Cdh11 4:intergenic:Gm8765-Gm22199 4:intergenic:Rab38-Gm15661 4:intron:Polq 4:intron:Sis 3:intron:Syt1 3:intergenic:Gm26894-9530036O11Rik 4:intron:Kat6a 4:intron:Glo1 4:intergenic:Gm25644-Rpl31-ps18 4:intron:Mmd2 4:intergenic:Gm26146-Gm8226 4:intergenic:Ndst4-Ugt8a 3:intergenic:Tgfbr2-Rbms3 4:intergenic:Cadm1-Nxpe2 3:intergenic:Tacr3-Gm24048 4:intron:Lrp6 4:intergenic:9030612E09Rik-Pdss2 4:intergenic:Gm22867-Gtdc1 2:intergenic:Fam105b-Fam105a 4:intergenic:Gm22828-Gm23960 4:intergenic:Vmn1r-ps6-Samd9l 4:intergenic:Il1rapl1-Gm8855 4:intron:Ndst4 3:intergenic:Lrrc52-Rxrg 3:intergenic:Kctd16-Rps19-ps13 3:intergenic:Olfr677-Olfr678 4:intergenic:Gm22828-Gm23960 4:intergenic:Gm6317-Gm25383 4:intergenic:Gm24929-Gm9892 4:intergenic:AC166333.1-Scg2 4:intron:Efna5 4:intergenic:Spats2l-Gm17234 4:intergenic:Gm25476-Gramd3 4:exon:Mrrf 3:intergenic:Gm25335-Magi2 4:intron:Hs2st1 4:exon:Mlh3 4:intron:Sms 4:intron:Anks1b 4:intron:Mlip 4:intergenic:Gm12507-Klf4 4:intron:Cep85 3:intergenic:1700020N15Rik-Ids 4:intergenic:Gm12381-Gm25581 4:intron:Hoxa10 4:intergenic:RP23-394M5.1-RP23-433F5.5 4:intron:Kcnn2 4:intron:Agbl4 4:intergenic:Gm15614-Yes1 4:intergenic:Fgf10/Gm16263-Fgf10 4:intergenic:Sema5a-Sdc2 4:intergenic:Dusp6-B530045E10Rik 4:intergenic:Gm20732-Gm25987 4:intergenic:Gm26489-1700028P15Rik 4:intergenic:Gm26076-Gm25519 3:intergenic:Ank2-Ank2/Gm16958 4:intergenic:Gm25215-Epc2 4:intergenic:Gm24295-Mef2c 4:intron:Galntl6 4:intergenic:Tmpo-Rmst 3:intergenic:Gm1968-4632428C04Rik 4:intergenic:Camk2d-Gm15551 3:intergenic:Setbp1-Gm25824 2:intron:Clint1 4:intergenic:4930470P17Rik-Gm14264 4:intron:Zzz3 3:intergenic:Gm22495-Dtna 4:intergenic:Ltv1-Phactr2 4:intergenic:Nav3-E2f7 4:intron:Gabra2 4:intron:Specc1 3:intergenic:Tpi-rs2-Veph1 4:intron:Zkscan16 4:intergenic:Gm8321-Inhbb 4:intron:A830018L16Rik 3:intergenic:Ube2e2-Gm22499 4:intergenic:Gm23292-Gm25247 4:intergenic:Gm12479-Zfp462 4:intergenic:5930403N24Rik-Gm26535 4:intergenic:Gm23987-Fam135b 4:intron:Nck2 4:intergenic:Gm17490-Il2ra 4:intergenic:Plxnc1-Gm24186 4:intron:Smarcad1 4:intron:Stx17 4:intergenic:3110052M02Rik-Gm10509 4:intergenic:Tapt1-Ldb2 3:intergenic:Stxbp5l-Gtf2e1 4:intron:Kctd5 4:intergenic:Gm10295-Mctp2 4:intron:Sptan1 4:intergenic:Gm24319-Psat1 4:intergenic:Npy-Gm21786 4:intergenic:Gm8730-Cdh5 4:intergenic:Gbf1-Gm8974 4:intron:Unc13d 4:intergenic:Cdh26-Gm14314 4:intergenic:Gm24839-Tmem132c 4:intergenic:Nlrp4f-Gm10775 4:intron:Apba2 4:exon:Prdm11 4:intergenic:4930564D02Rik-1700095B22Rik 3:intergenic:Gm5944-Gm14903 4:intergenic:Cdh9-Gm22032 4:intergenic:Ccr3-Ccr2 4:intron:Gm9285 4:intergenic:Gm9312-Gm16372 4:intergenic:Gm4425-Gm20486 4:intergenic:Hpcal1-B430203G13Rik 4:intergenic:Gm6802-Gm9257 4:intergenic:Gm3993-Gm10479 4:intergenic:Gm3944-Gm5784 4:intergenic:Gm21989-Gm21989/Gm5977 4:intron:Gm10479 4:intergenic:Gm9257-Gm22189 4:intergenic:Gm9257-Gm22189 4:intergenic:Gm3993-Gm10479 4:intergenic:Hpcal1-B430203G13Rik 4:intergenic:Gm3944-Gm5784 4:intergenic:Gm10479-1700030C10Rik 4:intergenic:Gm4419-Gm5633 4:intergenic:Gm3993-Gm10479 4:intron:2410018L13Rik 4:intergenic:Gm23913-Gm10480 4:intergenic:Gm3944-Gm5784 4:intergenic:Gm23913-Gm10480 4:intron:3110053B16Rik 4:intron:9030624G23Rik 4:intergenic:Gm17391-2410018L13Rik 4:intergenic:Gm5633-Gm22908 4:intergenic:Gm23881-Gm3534 4:intron:Cacng3 4:intergenic:Oxgr1-Mbnl2 4:intergenic:Gm25656-Cntn6 4:intergenic:Gm22159-Gm25293 4:intergenic:Gm24122-Gm23036 4:intergenic:Gm16046-Tcte2 4:intergenic:Lin7a-Myf5 4:intron:Ank2 4:intron:Atp2c2 4:intergenic:Mtpn-Gm23273 4:intron:Bre 4:intergenic:Sema6d-4930583P06Rik 4:intergenic:Gm13466-Gm23072 4:intron:Nid1 4:intergenic:Shc3-Cks2 4:intergenic:Gm23987-Fam135b 4:intergenic:Gm26022-Chl1 4:intergenic:Gm13052-Gm13018 4:intergenic:Gm13421-Rxra 4:intergenic:Stim2-Gm24235 4:intergenic:Wdr43-Fam179a 4:intergenic:Gm24229-Rpl27a-ps2 4:intergenic:Gm11997-Vwc2 4:intergenic:Pbx1-Pbx1/AC096625.1 4:intergenic:Dok6-Gm22694 3:intergenic:Gm24062-Gm10259 2:intron:Mmp16 3:intergenic:Gm18360-Vmn2r70 4:intron:Nos1ap 4:intron:Ftcd 4:intergenic:Gm23531-Gm26181 3:intergenic:Sntg1-Gm24276 4:intron:Ubap2l 4:intergenic:4933409G03Rik-4932414N04Rik 4:intergenic:9230114K14Rik-Gm25601 4:intergenic:5330411J11Rik-Gm13551 4:intron:Ntm 4:intergenic:Ran-Gpr133 4:intergenic:Zfp317-Olfr857 4:intergenic:Olfr58-Olfr859 4:intergenic:Olfr857-Olfr58 4:intergenic:Rab27b-Dynap 4:intron:Sv2b 4:intergenic:Lyrm9-Nos2 4:intergenic:Dlg3-Tex11 4:intergenic:Gm15083-Slc6a11 4:intergenic:Gm10167-Mtus2 4:intergenic:mmu-mir-6348-mmu-mir-6354 4:intergenic:Gm14372-Shank2 4:intergenic:Gm23733-6530403H02Rik 4:intergenic:Cadm2-Gm24681 4:intergenic:Gm13853-Lrguk 4:intergenic:Gm15581-Gm25021 4:intron:Mtbp Off-target primers show all... show less...
115 / rev	`TATTGCTAAGCCATAAATGT GGG` `.................... ...` Enzymes: BstXI, SmiMI Cloning / PCR primers	69	88	55	36	73	83	0 - 0 - 1 - 21 - 129 0 - 0 - 0 - 2 - 1 151 off-targets	3:intergenic:Gm5257-mmu-mir-6343 4:intergenic:Ccdc50-Fgf12 4:intergenic:Ccser1-Gm22212 4:intergenic:Gm11231-Gm11232 4:intron:Cdc23 4:intergenic:Gm24680-4921530L21Rik 3:intergenic:Csgalnact1-Gm15654 4:intergenic:Gm22684-Anxa1 3:intergenic:Epha4-Gm26263 4:intergenic:Gm23781-Gm25610 3:intron:Rnf19a 4:intergenic:Gm23829-Gm5974 4:intergenic:Rpl31-ps8-Tcea1-ps1 3:intron:Sox6 4:intergenic:Anapc15-ps-4732465J04Rik 4:intron:Kcnh7 4:intergenic:Gm24127-Pcdh9 4:intergenic:Gm26184-Dlgap2 4:intergenic:Gm15297-Hmgn2l6 4:intron:Ptpn22 4:intron:Nsf 4:intron:Glt8d2 4:intergenic:Gm26256-n-R5s61 4:intergenic:Gm5742-Gm22223 4:intergenic:Gm23059-Gm7745 4:intergenic:Gm12641-Gm12642 4:intron:Doc2b 4:intron:Fer 4:intron:Mamdc2 4:intron:Cdkal1 4:intergenic:Gm26076-Gm25519 4:intergenic:4930554G24Rik-Gm12005 4:intergenic:Gng2-Saysd1 4:intergenic:Zfp446-Slc27a5 4:intergenic:Gm24295-Mef2c 4:intron:Apbb2 3:intergenic:Gm7931-Slit2 4:intron:Sipa1l1 4:intergenic:Gm26454-Gm7456 4:intron:Ncapg2 4:intergenic:Gm14903-Gm14906 4:intergenic:Gm11077-Pde3a 4:intergenic:Gm23203-Gm23447 4:intergenic:Olfr582-Olfr583 4:intron:Xdh 4:intergenic:Gm21750-Gm24540 4:intron:Enox2 4:intergenic:Ppargc1a-mmu-mir-6417 4:intergenic:Gm25727-F830104G03Rik 4:intergenic:Gm14859-Cited1 4:intergenic:Gm5507-Redrum 4:intergenic:Plin2-Dennd4c 4:intergenic:Gm5776-Luzp2 4:intron:Etl4 4:intergenic:Lrrtm1-Ctnna2 4:intron:Kcp 4:intergenic:Gm25485-Gm12460 4:intron:Ahrr 4:intron:Nln 4:intergenic:Gm13913-Gm13914 4:intergenic:Gm12367-Gm12368 4:intron:Rnf145 4:intergenic:Igkv9-123-Igkv1-122 4:intergenic:Gm5455-Gapt 4:intron:Nebl 4:intergenic:Gm26853-Gm21769 4:intergenic:1700017G19Rik-Intu 4:intron:Dyrk1a 4:intergenic:Rpsa-ps10-Mir3963 4:intergenic:Gm5611-Chordc1 4:intron:Frmpd3 4:intron:Garnl3 4:intergenic:Mettl21a-2810408I11Rik 4:intron:Thsd7b 3:intergenic:Gm21614-Gm23249 4:intergenic:Gm17333-Gm25025 4:intergenic:Speer2-Gbe1 4:exon:Slco1b2 3:intergenic:Fgf10-Nnt 4:intergenic:Slc6a3-Clptm1l 4:intergenic:Amer2-Spata13 4:intron:8430427H17Rik 4:intergenic:Fam180a-Mtpn 4:intron:Lmbrd2 3:intergenic:Zeb1-Arhgap12 4:intergenic:Slc8a1-Rpl31-ps25 4:intergenic:Sorcs3-AC116451.1 4:intron:4930564K09Rik 4:intron:Skap1 4:intergenic:Ubr5-Odf1 4:intergenic:Mir133a-2-Slco4a1 4:intergenic:Gm5257-mmu-mir-6343 3:intron:Chst11 3:intron:Pcdh7 4:intergenic:Gm10476-Gm17391 4:intergenic:Gm10330-Gm17330 4:intron:1700007B14Rik 3:intron:Nfasc 4:intergenic:Myl9-Gm14231 4:intergenic:Gm21614-Gm23249 4:intergenic:Gm25811-Adamtsl1 3:intergenic:Gm11911-Gm11907 4:intron:Nckap5l 3:intron:Dock3 4:intergenic:Gm13577-Gm13576 4:intergenic:Brms1l-Gm22296 4:intron:Pard3b 4:exon:Rtdr1 4:intergenic:Gm22438-Gm22159 4:intergenic:Mical3-Pex26 4:intron:Itpr2 4:intron:Cpeb1 4:intergenic:Enox2-Gm14696 4:intergenic:Gm13810-B230118H07Rik 4:intron:Thsd7a 4:intron:Dmtf1 4:intergenic:Gm7191-Gm23494 4:intergenic:Olfr1141-Olfr152 4:intergenic:Gm16685-Gm24614 3:intergenic:Gm22619-Gm12650 3:intergenic:Gm22619-Gm12650 3:intergenic:Scml4-Sobp 2:intron:Ctnna2 4:intergenic:RP24-338F2.1-Tac1 3:intron:Clasp2 4:intergenic:Gm10205-4930435H24Rik 4:intergenic:Tenm2-Gm12128 4:intron:Grm7 4:intergenic:Gm25684-Gm9966 4:intergenic:Gm9931-Gm22966 3:intron:Myo1d 3:intron:Gapvd1 3:intergenic:Gm8116-Col12a1 4:intron:Asah1 4:intergenic:Sorcs1-Gm26629 4:intergenic:Gm15762-B230104C08Rik 4:intergenic:Gm22544-Gm22509 4:intergenic:Etv6-Bcl2l14 4:intergenic:Irx5-Irx6 4:intron:Styxl1 4:intergenic:Gm25258-Hao1 4:exon:Derl1 4:intergenic:Gm22507-Trhde 4:intergenic:Slc7a13-Atp6v0d2 4:intergenic:Gm22827-Gm22181 4:intergenic:Maf/Gm15655-Dynlrb2 4:intron:Zc3h12b 4:intron:Arih1 4:intergenic:Gm12377-Gm12376 4:intergenic:Gm4868-Gm23151 4:intron:Spag9 Off-target primers show all... show less...
306 / rev	`AATGGCAAGACTCTGTGGTA AGG` `.................... ...` Cloning / PCR primers	69	82	51	57	62	65	0 - 1 - 2 - 23 - 157 0 - 0 - 0 - 1 - 5 183 off-targets	4:intergenic:Efnb1-Gm14812 4:intergenic:Bbs9-Bmper 3:intron:Cep112 4:intergenic:Scap-5830462I19Rik/Scap 4:intergenic:Gm24591-Gm26241 4:intron:Adam12 4:intergenic:6030469F06Rik-Gm24613 4:intergenic:Pisd-ps2-Scaf8 3:intergenic:Hmgn2-ps1-Epha5 3:intergenic:Gm5593-Vmn2r60 4:intron:Dnah5 4:intergenic:Gm16578-Cplx2 3:intron:Mast4 4:intron:Dab1 4:intergenic:A930039A15Rik-Gm25336 4:intergenic:Gm24680-4921530L21Rik 4:intergenic:Cst10-Gm24551 3:intergenic:Myl10-Col26a1 4:intergenic:4930569F06Rik-Suds3 4:intergenic:Gm24104-Gm26058 3:intron:Rnf135 3:intergenic:Gm10797-Zfp365 4:intergenic:C1ql2-Marco 4:intergenic:Gm22499-Ube2e2 4:intergenic:Cyp2b13-Cyp2b9 4:exon:Nlrc3 3:intergenic:Bdh1-Gm26053 4:intron:Rnls 4:intergenic:Anapc15-ps-4732465J04Rik 4:intergenic:Pabpc1-Ywhaz 4:intergenic:Cbx3-ps3-Ccdc171 4:intergenic:Gm14601/Gm15482-Gm14644 4:intergenic:Gm12105-Gm12106 4:intergenic:Gm25261-Gm831 3:intergenic:4930428N03Rik-Gm10398 4:intergenic:Usp39-0610030E20Rik 4:intergenic:Gm5611-Chordc1 4:intergenic:AY702102-A630075F10Rik 4:intron:Cntn1 4:intergenic:Mesp1-Mesp2 4:intergenic:Gm11542-Gm11541 4:intergenic:Gm10000-Dicer1 4:intergenic:Ttll7-Gm23131 4:intron:Ano2 4:intergenic:D16Ertd472e-Gm24861 4:intergenic:Gm23986-Gm24929 4:intergenic:Gm24845-Tcf4 4:intergenic:Gm20396-Rbms3 4:intergenic:Sgcd-Gm23039 4:intron:Shank3 4:intergenic:1700029N11Rik-Jarid2 4:intergenic:Scgb2b15-Gm25817 4:intergenic:Gm9149-Gm24766 4:intergenic:Scgb1b7-Gm5329 4:intergenic:Cstf2t-Prkg1 4:intergenic:Efna5-Fbxl17 4:intron:Dennd2a 4:intron:Kndc1 4:exon:Pdx1 4:intron:Ifit2 4:intergenic:Cypt2-Gm14823 4:intergenic:Erbb4-Gm16076 4:intergenic:Gm14263-Gm14266 4:intron:Dock4 4:intergenic:Gm13484-Gm13498 4:intron:Specc1 4:intron:Ppp1r12b 4:intergenic:Ywhae-Doc2b 3:intergenic:Enpep-Elovl6 3:intergenic:Gm22191-Gm22726 2:intergenic:Ldoc1l-Prr5 4:intergenic:1700020N15Rik-Ids 4:intergenic:Gm23337-Gm26081 4:intergenic:Gm15810-Phactr1 4:intergenic:Trpm3-C330002G04Rik 4:intergenic:Ncald-Gm15942 4:intergenic:Npas3-Egln3 4:intron:Map2 4:intergenic:Gm23104-Hsp25-ps1/Gm9817 3:intergenic:Asb13-Calml3 3:intergenic:Gm4297-AL672071.1 4:intergenic:Aim1/Gm10812-Speer5-ps1 4:intron:Scn5a 4:intergenic:Gm9836-Kcnh2 4:intergenic:Slc35f3-Coa6 4:intron:A230108P19Rik 4:intergenic:A530072M11Rik-Gm11863 4:intergenic:Gm4799-Chst11 4:intron:Ablim2 4:exon:Uba7 2:intergenic:Anapc4-Gm23532 4:intergenic:Gm26580/Kcnq5-Rims1 4:intron:Lrrn2 4:intron:Cbl 4:intergenic:Fam107b-Gm13185 4:intergenic:Rps24-ps2-Gm8775 4:intergenic:Ssxa1-Gm1848 4:intergenic:Gm13761-Olfr1226 4:intergenic:Gm23884-Gm25684 4:intergenic:Itprip/Wdr96/Gm22240-Ccdc147 4:intron:Col22a1 4:intron:Prpf40b 4:intron:Lrrc49 4:intergenic:Gm25496-Ryr2 4:intergenic:Ceacam16-Ceacam19 4:intergenic:Dusp10-Gm23349 4:intron:Gria1 4:intron:Evi5l 4:intergenic:Epha4-Gm26263 1:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Zfp781-Zfp873 4:intergenic:Gm24127-Pcdh9 4:intergenic:1700019M22Rik-Gm26480 3:intron:Vmn2r23 4:intergenic:Mall-Nphp1 4:intron:Scara3 4:intron:Rabepk 4:intron:Zfp407 4:intergenic:Cdh10-Gm25976 4:intergenic:Ndfip1-Spry4 4:intergenic:Gm13680-Gm13679 4:intron:Gab1 4:intron:Dhcr24 4:exon:Rbm11 4:intron:Ttc23 4:intergenic:3100003L05Rik-Gm21957 4:intergenic:Gm24484-Fth1 3:intron:Fndc1 4:intergenic:Rpl30-ps8-Foxn3 4:exon:2310003H01Rik 4:intergenic:Gm11467-Gm11468 3:intergenic:Gm7224-Vmn2r59 4:intron:Ptprg 4:intron:Pde4d 4:intergenic:Itgb2l-Pcp4 4:intron:Iqcj 3:intergenic:Gm6851-Gm24910 4:intron:Ppp1r14a 4:intron:Mrps31 4:intergenic:Lats2-Sap18 4:intergenic:AC121988.1-Gm25740 4:exon:Chrm2 4:intergenic:Gm14219-Gm11445 3:intergenic:Bin1-Gypc 4:intergenic:Gm16065-Tbx5 4:intergenic:Cadm2-Gm24681 4:intergenic:Kifc3-Cngb1 4:intergenic:Gm26556-Ppp1r9a 4:intron:Bbs9 4:intergenic:Mir2137-Zfp185 4:intergenic:Ppp4r2-Pdzrn3 3:intergenic:Rpl21-ps5-Gm24508 4:intergenic:Arhgap12-Kif5b 4:intergenic:Gm8839-Mageb10-ps 4:intergenic:Gm7350-E330010L02Rik 4:intergenic:E330010L02Rik-Gm14533 3:intergenic:1700061I17Rik-D3Bwg0562e/Gm22408 4:intergenic:Gm16418-Gm4846 4:intergenic:Gm25714-Gm24997 4:intergenic:Atp8b1-Gm23016 4:intergenic:Gm25715-N6amt1 3:intergenic:Dclk1-Gm25132 4:intergenic:Frmpd4-Gm8832 4:intergenic:Ensa-Mcl1 4:intron:2310002F09Rik 4:intergenic:Gm25422-Tmtc2 4:exon:Ipo4 4:intron:Zfp568 4:intergenic:Gjb6-Cryl1 4:intergenic:Syf2-Gm16225 4:intron:Pik3r1 4:intergenic:Gm16223-Cpeb2/Gm7854 4:intergenic:Gm23986-Gm24929 4:intergenic:Fam102b-Slc25a54 3:intergenic:Gm5514-Trpm3 4:intergenic:Auts2-Gm25722 4:intergenic:St8sia4-Gm22360 3:intergenic:Mpp7-Gm24292 4:intergenic:Gm22183-mmu-mir-378d 4:intergenic:D3Ertd751e-Gm25714 4:intergenic:Gm22825-Zfhx3 4:intergenic:Gm22628-5033411D12Rik 4:intergenic:Gm5611-Chordc1 Off-target primers show all... show less...
769 / fw	`CTAATTGTATGGAACTTCAT TGG` `.................... ...` Enzymes: SmiMI, BtsCI Cloning / PCR primers	69	86	32	33	50	72	0 - 1 - 4 - 23 - 93 0 - 0 - 0 - 4 - 1 121 off-targets	3:intergenic:Gm26861-Gm25496 4:intergenic:Cntnap5b-Gm25537 3:intergenic:Sh3bp4-Agap1 4:intergenic:Tbc1d1-Gm25306 4:intergenic:Gm22011-Mthfr-ps1 4:intergenic:Gm26101-Plscr5 4:intron:Cwc27 3:intron:Hmcn1 4:intergenic:Gm5383-Mir221 4:intergenic:Fam184a-Man1a 4:intron:Klhl1 4:intron:Kcnd3 4:intergenic:Gfra2-Gm9192 4:intergenic:Mc4r-Gnal 4:intergenic:Htr1a-Dph3b-ps 4:intergenic:Olfr733-Olfr734 4:intergenic:Gfra2-Gm9192 4:intergenic:Rpl10l-Mdga2/MDGA2 3:intergenic:Nox3-Gm23186 4:intergenic:Gm23185-Gm23848 4:intron:Klf12 4:intergenic:Gm5514-Trpm3 4:intron:Wdr25 3:intron:Ctnna1 4:intergenic:Gm20594-Gm4409 4:intron:A1bg 4:intergenic:Mark3-Ckb 3:intergenic:Syt14-Gm23583 4:intergenic:Krtap5-3-Gm7579 4:intergenic:Gm22764-Gm4822 4:intergenic:Gm22618-Gm22976 4:intron:Pdilt 4:intergenic:Gm25248-Gm26326 4:intron:A230006K03Rik 4:intergenic:Bche-Gm6098 3:intron:Dlg1 3:intergenic:Eif3s6-ps1-Gm11995 4:intergenic:Olfr1040-Olfr1042 4:intron:Il18bp 4:intergenic:Etl4-Gm13328 4:intergenic:Ctnna3-Gm22829 4:exon:Dip2b 3:intergenic:Cntnap4-Gm25766 4:intron:Gpr141 4:intergenic:Gm13070-Spsb1 4:intergenic:Pdgfc-Gm22531 4:intergenic:Gm20091-Gm8601 3:intron:Bckdhb 4:intergenic:Wac-Gm5819 4:intergenic:Gm26137-Gm23733 4:intergenic:Tlr2-D930015E06Rik 4:intron:Tbl1xr1 4:intergenic:Gm24062-Gm10259 1:intergenic:Crbn-Gm22418 4:intron:2810055G20Rik 4:intron:Rcan2 4:intergenic:Gm9008-Ctnna2 4:intergenic:Gm10797-Zfp365 3:intergenic:Gm5329-Gm9135 3:intergenic:Gm24766-Gm4398 3:intergenic:Gm25817-Scgb2b17 4:intergenic:Bckdhb-Gm26146 4:exon:Mitf 3:intergenic:Cdh26-Gm14314 3:intron:Rtkn2 4:intergenic:Ppargc1b-Gm25834 4:intergenic:Gm15527-Chn2 3:intergenic:Gm14574-Gm14576 4:intergenic:Ppp1r2-ps2-Nup210 4:intergenic:Gm10254-Sertm1 4:intron:Erbb4 4:intron:Lrrc16a 4:intron:Clint1 4:intergenic:Gm14631-Gm25023 4:intergenic:Gm14668-Gm14671 4:intergenic:Pigr-Faim3 2:intergenic:Gm5594-Vmn2r-ps59 2:intergenic:Amd2-Hs3st5 4:intergenic:Gm23217-Gm24390 4:intergenic:Mir652-Tmem164 3:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:Rhov-Gm14175 4:intergenic:Epha6-Gm9017/Epha6 4:intergenic:Gm23477-Gm10780 4:intergenic:Tmem26-Gm24172 3:intergenic:Htr2c-Gm24627 4:intron:Cdh13 3:intergenic:Tspan18-Gm23380 3:intergenic:Kctd16-Rps19-ps13 4:intergenic:Gm13046-Gm13044 4:intergenic:Gm5860-Nfib 4:intergenic:Gm23892-Gm25382 4:intergenic:Impg1-RP24-325L16.1 4:intron:Pde1a 4:intron:5730522E02Rik 3:intergenic:Gm8349-Arhgef26 4:intron:Sdccag8 4:intergenic:Gm13483-Gm13486 4:intron:Ank 4:intergenic:Gm26126-Gm11114 4:intergenic:Gm25875-Gm23835 4:intron:Cftr 4:intron:Slc37a1 4:exon:Plekho2 4:intergenic:Prex2-A830018L16Rik 4:intron:Pebp4 4:intergenic:Gm25454-n-R5s165 4:intergenic:Robo2-Gm26307 3:intron:Fryl 4:intergenic:Fam19a2-Gm23777 4:intergenic:Gm20713-Gpc5 4:intergenic:Tenm2-Gm12128 4:intron:Zfp438 4:intergenic:Scgb1b3-Scgb2b7 4:intergenic:Gm11932-Bach2 3:intron:Ppm1h 4:intergenic:Gm6373-Gucy2f 4:intergenic:Gm24720-Tshz1 2:intergenic:Zic4-Gm26101 2:intergenic:Gm25787-Gm23345 4:intergenic:Sec61b-NAMA_1 Off-target primers show all... show less...
20 / rev	`GGGGCTGTGGATAAACTCAA GGG` `.................... ...` Enzymes: SmoI Cloning / PCR primers	68	81	69	74	72	81	0 - 0 - 5 - 30 - 156 0 - 0 - 0 - 3 - 5 191 off-targets	2:intergenic:Gm23728-Foxb1/B230323A14Rik 3:intergenic:Gm12377-Gm12376 3:intergenic:Gm15794-Nsun7 4:intergenic:D630002J18Rik/Gipc2-Dnajb4 4:intergenic:Lrrtm4-Gm26264 4:intergenic:Mn1-C130026L21Rik 3:intergenic:Gm9242-Gm24777 4:intergenic:4631405K08Rik-Plxna2 4:intron:Stat6 4:intergenic:Vmn2r-ps115-Vmn2r-ps116 4:intron:Mecr 4:exon:Ncr1 4:intron:Cacna1h 3:intergenic:Klhl2-Gm26210 4:intergenic:Anks1b-Apaf1 4:intergenic:Frs3-Pgc 4:intron:Greb1 4:exon:A930011O12Rik/Mir124a-1/Mir3078 4:intergenic:Runx1-AC124326.1 4:intron:Tpk1 4:intergenic:AF529169-Tmed3 4:intergenic:Gm26076-Gm25519 3:exon:Syt7 2:intron:Dlc1 3:intergenic:Gm15529-Gm5300 4:intron:Sh3bp2 4:intron:Ptprg 3:intron:Sgk1 4:intron:Supv3l1 4:intron:AB124611 4:intergenic:Xpo4-Lats2 4:intergenic:Olfr284-Olfr283 4:intergenic:Stac-Arpp21 3:intergenic:Gm26054-Xylt1 4:intron:Kank4 4:intron:9430007A20Rik 4:intron:Nav1 4:intron:C430042M11Rik 4:intron:Sh3bp5 4:intron:Myo16 4:intergenic:Gm25672-Barhl2 4:intergenic:4930555F03Rik-Gm23986 4:intergenic:Vmn1r-ps60-Vmn1r77 4:intergenic:Vps13c-Gm22382 3:intron:Kcnd2 4:exon:Abcg5 4:intergenic:Clec14a-Gm17529 4:intron:Scn8a 4:intergenic:Gm5839-Gm25440 4:intron:Camk2a 3:intergenic:Fkbp5-Gm20109 4:intron:Ap5z1 3:intergenic:Tubb4b-ps1-4921511H03Rik 4:intergenic:Irx1-Rpl9-ps4 4:intergenic:Entpd1-Ccnj 3:intergenic:Gm13211-Gm13254 4:intergenic:Lrrc58/Gm15725-Gpr156 4:exon:Scube3 4:intergenic:Gm10479-1700030C10Rik 3:intergenic:Sp4-Gm25577 4:intergenic:Vrtn-Syndig1l 4:intergenic:Gm9157-Ftx 4:intergenic:Gm9157-Ftx 4:intergenic:Tshz2/Gm26883-Gm26883 4:exon:Calu 4:intergenic:Ppp1r15b-Plekha6 4:exon:4921536K21Rik 4:intergenic:Olfr1467-Olfr1469 4:intergenic:Rybp-Gm23234 4:intron:Grm5 3:intron:Disc1 4:intergenic:Nox3-Gm23186 4:intron:Adrbk2 4:intergenic:Gm25658-Atp2b1 4:intron:Park2 4:intergenic:Gm25884-Gm22582 4:intergenic:Sntb1-Has2 4:intron:BC006965 3:intergenic:Scgb2b26-Scgb1b26-ps 4:intron:Pold3 4:intron:Nr5a2 4:intergenic:Vmn1r80-Vmn1r-ps64 4:intron:Lrba 4:intron:Amph 4:exon:Gm15124/Gm15123 3:intergenic:Mir344-A330076H08Rik 4:exon:A830018L16Rik 4:intron:Cntln 4:intron:Ispd 4:intron:Uox 4:intergenic:Gabrg1-Gabra2 4:intergenic:Rps6ka1-Hmgn2 3:intron:Rab9b 4:intron:Lekr1 4:intergenic:Foxp2-Mdfic 3:intergenic:Gm6395-Gm527 4:intergenic:Adss-Gm16432 4:intergenic:Scgb2b27-Scgb1b27 4:intergenic:Scgb2b25-ps-Gm12757 4:intergenic:Etl4-Gm13328 4:intron:F13a1 4:intergenic:Gm12014-Cnrip1/Plek 4:intergenic:Gm12763-Rpl23a-ps12 4:intergenic:5830454E08Rik-Gm23624 4:intergenic:Slc22a23-Pxdc1 4:intron:Blm 4:intron:Xylt1 4:intergenic:Slc9a7-Gm14529 4:intergenic:Gm25051-Gm1818 4:intergenic:Nucb2-n-R5s157 4:intergenic:Hivep1-Edn1 4:intergenic:Gm23217-Gm24390 3:intergenic:Jmjd6/Mettl23-Mettl23 4:intergenic:4930554G24Rik-Gm12006 4:intergenic:Tenm1-Gm24763 4:intergenic:Cadm2-Gm15828 4:intergenic:4930431F12Rik-Gm10205 4:intergenic:Gm13791-Gm13792 4:exon:Col15a1 4:intron:Brwd3 4:intergenic:Gm6463-Gm25813 4:intron:Phactr3 4:intergenic:Gm20732-Gm25987 4:intergenic:Gm24626-Cenpc1 4:intron:Col23a1 3:exon:Arr3 3:intergenic:Gm25258-Hao1 4:intron:D030068K23Rik 4:intergenic:Cmip-Plcg2 4:intron:Ikzf1 4:intergenic:Noxa1-Gm13387 4:intron:RP23-304I16.1 3:exon:Spata2 4:intergenic:Gm13580-Pla2r1 2:intergenic:Sybu-Sybu/2310069G16Rik 4:intergenic:Spata19-Opcml 3:intergenic:Epas1-Tmem247 4:intergenic:Hs3st4/Gm27040-Gm25759 4:intergenic:Gm24957-Gm22492 4:intergenic:Cdh17-Gm22477 4:intron:Kcnq3 4:intergenic:Syngr1-Tab1 4:intron:Gucy2g 4:intron:Col14a1 4:intron:Dnah2 4:intron:Ethe1 4:intergenic:Gm24731-Gm22076 4:intergenic:Gm17070-Ptgdr 4:intron:Pitpnc1 4:intergenic:Gm23296-Akap6 4:intergenic:Gm26728/Cd9-Gm26728 4:intergenic:Slco6c1-D1Ertd622e 4:intron:BC052040 4:intron:Thada 3:intergenic:Scgb1b3-Scgb2b7 4:exon:Ucp1 3:intergenic:Cacna1c-Gm25905 4:intron:Akap12 4:intergenic:Gm9531-Gm26907 4:intergenic:Cx3cl1-Ccl17 4:intergenic:Ndst4-Ugt8a 4:intergenic:Klhl25-Gm5597 4:intergenic:Gm12106-Stc2 4:intergenic:A230028O05Rik-Tprg 4:intergenic:Gm23149-Gm14464 4:intergenic:Cd80-Timmdc1 3:intergenic:Gm2574-Gm24728 4:intergenic:Gja8-Gja5 4:intron:Ythdc2 4:intergenic:Gm25476-Gramd3 3:intergenic:Gnai2-Slc38a3 4:intergenic:Scarna3b-Dcaf5 4:intergenic:Ttf2-Gm12488 3:intergenic:Dennd4b-Gatad2b 4:intergenic:Cacna1h-Tekt4 2:intergenic:Gm26076-Gm25519 2:intergenic:Gm5593-Vmn2r60 4:intergenic:1190003K10Rik-Gm4810 3:intergenic:Gm5507-Redrum 4:intron:Prkcb 4:intergenic:Ppp3cb-Usp54/1810062O18Rik 4:intergenic:Oraov1-Oraov1/Ccnd1 4:exon:Ascc3 4:intergenic:Gm22543-AC154801.1 3:intergenic:Gm12509-Gm12508 4:intergenic:Vmn1r68-Vmn1r-ps49 4:intron:Orm3 4:exon:Naa60/1700037C18Rik 4:intergenic:Slc16a7-Lrig3 4:intron:Cep350 4:intergenic:Krt222-Krt24 Off-target primers show all... show less...
74 / rev	`CCAACAATCCTGAGCAATCA TGG` `.................... ...` Enzymes: NlaIII, MwoI Cloning / PCR primers	68	86	45	37	66	75	0 - 0 - 3 - 40 - 132 0 - 0 - 0 - 5 - 3 175 off-targets	4:intergenic:AL844604.1-Mpdz 2:intergenic:Gm7224-Vmn2r59 3:intergenic:Mir680-3-Gm26071 4:intergenic:Ankrd16-Gm13310 4:intron:Foxp2 3:intron:Col16a1 4:intron:Akap14 4:intergenic:Gm14569-Pgrmc1 4:intergenic:Bmp2k/Paqr3-Paqr3 4:intron:Adamts2 4:intron:Slc1a7 4:intergenic:Efcab4b-Prmt8 3:intergenic:Ubqln1-Gkap1 4:intergenic:Gm22438-Gm22159 3:intergenic:Caln1-Gm23761 3:intergenic:Lmo7-Gm22347 4:intron:Agbl4 4:intron:Atp13a3 4:intergenic:St18-Pcmtd1 4:intron:Mgat4c 4:intergenic:Lhfp-Nhlrc3/Gm16206 4:intergenic:Gm23896-Gm24902 4:intergenic:Ndfip2-4930449E01Rik 4:intron:Gabrg3 3:intergenic:Gm15204-Reps2 4:intergenic:Gm11189-Hspa4 4:intergenic:Fam189a1-Tjp1 4:intron:Gm13507 4:intergenic:Gm13816-Gm13791 4:intron:Cnksr3 4:intron:Cacna2d1 4:intergenic:1700018B24Rik-Gm23511 3:intron:Arid1a 4:intergenic:Abcb5-Itgb8 4:intron:Slc19a1 4:intergenic:Gm25578-Actr3 4:intergenic:Gm25522-Gm25587 4:intergenic:4933439G12Rik-Pip4k2a 4:intron:Spats2l 4:intergenic:Thnsl1-Gm13378 4:intergenic:D830013O20Rik-Tmem30b 4:intergenic:Gm24839-Tmem132c 4:intron:Shank1 4:intergenic:Ostc-Rpl34 4:intron:Phactr2 3:intergenic:Gm25792-Gm23453 4:intron:Adam12 4:intergenic:Gm22326-Sv2b 2:intron:Acvr1 4:intergenic:Stpg2-Gm24260 4:exon:Pik3r6 3:exon:Tango2 4:intergenic:Gpr149-Gm22433 4:intron:Mettl10 4:intergenic:Gm11996-Gm11997 4:intron:D930032P07Rik 3:intergenic:Gm13794-Gm13796 3:intron:Tenm2 4:intron:Prkg1 4:intron:Cdyl2 3:intergenic:AC158354.1-Gm4804 3:intergenic:Pot1a-Gm24691 3:intergenic:Gm9578-Gm22891 3:intergenic:Gm7700-1110059M19Rik 3:intergenic:Igsf1-n-R5s6 3:intergenic:Sall2-Gm26328 4:intergenic:Rpl30-ps11-Atp6ap2 4:intergenic:Ctnna2-Gm21045 4:intergenic:Rnf130-Gm12194 3:intron:Gm6999 4:intergenic:Gm25799-Slit3 4:intergenic:Gm11279-Gm11280 4:intron:Fgd4 4:intergenic:Dlg5-Gm17105 3:intergenic:Gm14603-Dcaf12l2 4:intron:Erbb4 4:intron:Megf11 3:intergenic:Dmtf1-Gm24666 3:intergenic:Nox3-Gm23186 4:intergenic:Gm24587-Dcn 3:intron:Dmd 4:intergenic:Akirin1-Rhbdl2 4:intergenic:Gm22104-Gm25550 4:intergenic:Cntnap4-Gm25766 4:intergenic:BC049702-Gm14531 3:intergenic:Gm24861-Chodl 4:intergenic:Gm20795-Gm20827 4:intergenic:Gm24587-Dcn 3:intergenic:Gm7456-Foxg1/3110039M20Rik 4:intergenic:Nkx1-1-Fam53a 4:intron:Bckdhb 4:intergenic:Gm9341-Gm24444 4:intergenic:Gm11680-BC006965 4:intergenic:Gm23831-1700017G19Rik 4:intergenic:Ankle2-Pgam5/Gm26718 4:exon:Mycs 4:intron:Trpm3 4:intron:9530036O11Rik 4:intron:Dpyd 4:intron:Kdm4c 4:intron:Edar 4:intergenic:Gm15202-Gm26317 4:intron:Dapk1 4:intergenic:Areg-Gm23092 3:intergenic:Vmn2r71-Vmn2r-ps73 3:intergenic:Gm8993-Gm9001 4:intergenic:Atoh8-Sftpb 4:intron:Plekha7 4:intergenic:Gm22434-Prrx1 4:intron:Kremen1 4:intergenic:Gm15405-Gm27039 4:intergenic:Gm26026-Gm5513 3:intergenic:Gm11241-Ptprd 3:intergenic:Nxph1-Gm24104 4:intron:Zfp618 4:intron:Lepr 3:intergenic:Gm2308-Vmn2r60 3:intergenic:Gm5594-Vmn2r-ps59 3:intron:Fbxo18 4:intergenic:Gm23975-Gm11848 4:intergenic:Brd3-Wdr5 3:intergenic:Gm16050-Gm8603 3:intergenic:Unc93a-Smok2a 4:intron:Gm13441 4:intergenic:Wisp3-Fyn 3:intron:6030443J06Rik 3:intron:Atp11c 4:intergenic:Camk1g-4631405K08Rik/Mir205 4:intergenic:Ntf3-Kcna5 4:intron:Cntnap5a 2:intergenic:Gm7847-Gm23674 4:intergenic:Tbc1d23-Tmem30c 4:intron:Sipa1l2 4:intergenic:Gm7731-Gm21897 4:intergenic:Errfi1-Park7 4:intergenic:Gm9578-Gm22891 4:intergenic:Metrn-Fbxl16 4:intergenic:Prdx2-rs3-Dhrs3 4:intergenic:Dock9-Ubac2 4:intron:4932438H23Rik 4:intergenic:Gm21779-Gm21247 4:intron:Gm21758 4:intergenic:Gm21805-Gm21241 3:intron:Hspg2 4:intergenic:Zbtb38-Zbtb38/E030011O05Rik 3:intergenic:Negr1-Gm15578 3:intergenic:Trio-Dnah5 4:intergenic:Jakmip1-Gm1043 3:intergenic:Gm11922-Map3k7 4:intergenic:Kl-Stard13 4:intron:Slc1a3 4:exon:Cntn4 4:intron:Nr3c2 3:intergenic:RP23-341H6.1-Lca5 4:intergenic:Gm17131-Tmem132d 4:intron:A1cf 4:intron:Elfn2 4:intergenic:Cbln2-Gm5096 4:intron:Thrb 4:intergenic:Gm20773-Gm20836 4:intergenic:Gm20851-Gm21660 4:intergenic:Gm20795-Gm20827 4:intergenic:Gm20795-Gm20827 4:intron:Mapk10 4:intron:Stambpl1 4:intergenic:mmu-mir-6342-Gm23771 4:intron:3110047P20Rik 4:intergenic:2210017G18Rik-Gm26814 4:exon:Ankle1 4:intergenic:Gm24919-Efcab2 4:intergenic:Gm9431-Gm14865 4:intergenic:Gm24720-Tshz1 4:intron:S100z 4:intron:Parvb 4:intron:Cntnap5a Off-target primers show all... show less...
653 / fw	`GTCTTTCCATCAAGTCCAAC AGG` `.................... ...` Enzymes: HinfI, MmeI, PfeI Cloning / PCR primers	68	89	45	23	62	70	0 - 1 - 3 - 16 - 134 0 - 0 - 0 - 2 - 2 154 off-targets	4:intron:Ncoa7 1:intergenic:Gm5593-Vmn2r60 2:intergenic:Gm7224-Vmn2r59 2:intergenic:Gm2308-Vmn2r60 3:intergenic:Tbc1d5-Satb1 4:exon:Erbb3 4:intron:Prdx1 4:intron:Twf1 4:intergenic:Wdfy3-Arhgap24 4:intergenic:Gm4345-Gm9041 4:intergenic:Gm9041-Gm26917 3:intergenic:Gm11214-Tnfsf15 4:exon:Pnrc2 4:intergenic:Ncoa2-AC121538.1 4:intron:Arid5b 3:intron:Heca 4:exon:Mdn1 4:intron:Catsperb 4:intron:Reln 4:intron:Ppp6r1 4:intergenic:Gm25460-Gm5263 3:intron:Slc2a3 4:intergenic:Inhbb-Ralb 4:intron:Trappc9 3:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Magi3-Gm23077 4:intron:Lrrc16a 3:intron:Plcb1 4:intergenic:Lyn/Gm26857-Gm26857 4:intergenic:4930556G01Rik-Gm25705 4:intron:Mroh3 4:intron:Setbp1 4:intergenic:Gm15000-Gm15001 3:intergenic:Gap43-4932412D23Rik 4:exon:Mrpl48/D630004N19Rik 4:intergenic:Mmp27-Mmp20 4:intron:Tmem194b 4:intergenic:4930555M17Rik-Pex2 4:intergenic:4933433F19Rik-Dusp26 3:intergenic:4921515E04Rik-C030047K22Rik 4:intergenic:Stag3-Gm10874/Got2-ps1 4:intron:Pard3b 4:intron:Depdc1b 4:intron:Rnf13 4:intron:Col24a1 4:intergenic:Gm26022-Chl1 4:intergenic:Spef2-Gm26350 4:intergenic:Igsf11-Gm24795 4:intergenic:Lrrc7-Gm9423 4:exon:Cyp4v3 4:intron:St3gal3 4:intron:Exd1 4:intergenic:Mucl1-Gm22852 4:intergenic:Gm12304-Stx8 4:intergenic:n-R5s6-Olfr1322 4:intergenic:Gm25722-Gm10051 4:intergenic:Ankdd1a-Plekho2 4:intron:Fgf13 4:intergenic:4930579O11Rik-Inpp4b 4:intergenic:Gm22628-5033411D12Rik 4:intergenic:Arap3-Pcdh1 4:intergenic:Fbxo15-Gm25453 4:intron:Grik2 4:intron:Ttn 4:intergenic:Gm11986-U7 4:intron:Arhgap26 4:intron:Vmn2r57/Gm20449 4:intron:Tcf7l2 4:intron:Atp5c1 4:intron:Fam185a 4:intergenic:Amotl1-Piwil4 4:intergenic:Rad51b-Zfp36l1/Gm26669 4:intergenic:Gm23092-Btc 4:intergenic:BC030500-Gm24847 4:intergenic:Gm23429-Gm24304 4:intron:AU019990 4:intron:Gabrg3 3:intron:Gm21989/Asap2 4:intergenic:AC175117.1-Hnf4g 4:intergenic:B230208H11Rik-Stx11 4:intergenic:Rap1gap2-Gm22993 4:intron:Zfp37 4:intergenic:D3Ertd751e-Gm25714 4:intergenic:Runx2-Gm16317 4:intergenic:Pdgfd-Ddi1 4:intergenic:Gm12667-Gm12669 2:intergenic:Gm22273-Gm24079 4:intergenic:Gm11890-Gm11892 4:intergenic:Ascl5-Cacna1s 4:intron:Sobp 4:intron:Nkain2 4:intergenic:Bod1-D630024D03Rik 3:intron:Slc16a7 4:intergenic:Fam19a2-Gm23777 4:exon:Gm10392/Abhd15 4:intron:Agap1 4:intron:Kcnip3 3:intergenic:Lce3a-Lce3b 4:intergenic:Gpr149-Gm22433 4:intergenic:Gm6686-Chd1 4:intergenic:Gm22062-Gm15224 3:intergenic:Maf/Gm15655-Dynlrb2 4:intergenic:Ppargc1a-mmu-mir-6417 4:intron:Tle1 4:intergenic:Mctp2-Gm10294 3:intergenic:Gm25778-Ppapdc1a 4:intron:Fbxl2 4:intron:0610040J01Rik 4:exon:Zfp706 4:intergenic:2310035C23Rik-Tnfrsf11a 4:exon:Bgn 4:intergenic:Gm5403-Mdm4-ps 4:intergenic:Gm22674-Cdyl 4:intergenic:Gm22825-Zfhx3 4:intergenic:Glrx3-Gm25798 4:intergenic:Uaca-Gm10655 4:intergenic:Rap2a-Gm17613 4:intergenic:Alcam-Gm25723 4:intron:L3mbtl4 4:intergenic:Gm10051-Gm15627 4:intergenic:Gm23681-Peli1 4:intron:Igf2bp3 4:intergenic:Gm24696-Myc 4:intergenic:Pax6os1-Pax6 4:intron:Adamts6 3:intergenic:Btbd10-Pth 3:intergenic:Gm25578-Actr3 4:intergenic:Csgalnact1-Gm15654 4:intergenic:Zfp330-Rnf150 4:intron:Gm14635 4:intergenic:Gm15442-Gm24749 4:intergenic:Fam189a1-Tjp1 4:intergenic:Gm8730-Cdh5 4:intron:Cacna1c 4:intergenic:Atg10-A830009L08Rik 4:intergenic:Eif5al3-ps-Cox18 4:intergenic:Stat4-Stat1 4:intergenic:Gm22198-Gm26975 4:intergenic:Gm12059-1700061J23Rik 4:intergenic:Gm24221-Sstr4 4:intergenic:Gm16510-Gm26558/Gorasp2 4:intergenic:Fam105a-Trio 3:intron:Lrba 4:intron:4930465A12Rik 4:intergenic:Mir3092-Fndc3b 4:intron:Grid1 4:intron:Afap1l1 4:intergenic:Upf1-Comp 4:intergenic:Olfr186-Olfr187 4:intergenic:Gm379-Brwd3 4:intergenic:Cdh10-Gm25976 4:intergenic:Mdga2/MDGA2-n-R5s60 4:intergenic:Gapt-Plk2 4:intergenic:Cs-Gm23182 Off-target primers show all... show less...
392 / fw	`ATATTACATCAGATAAAGAT TGG` `.................... ...` Low GC content Enzymes: BspPI, MflI, BamHI, NlaIV, NdeII Cloning / PCR primers	66	82	47	31	72	76	0 - 1 - 5 - 26 - 212 0 - 0 - 1 - 0 - 1 244 off-targets	4:intergenic:Pramef6-Gm13100 4:intergenic:Gm13058-Gm13080 4:intergenic:Rps8-ps1-Gja1 4:intron:Gm15155 4:intergenic:Gm12026-Gm12027 4:intron:4921528I07Rik 4:intergenic:Susd5-4921528I07Rik 4:intergenic:Gm24246-Pik3c3 3:intergenic:Gm4810-Cntnap3 4:intergenic:Gm23666-U7 4:intergenic:Rps19-ps8-Mtf2 4:intron:Ube2a 4:intergenic:Gm2373-5330416C01Rik 4:intron:Skint5 4:intergenic:Gm24275-Gm12291 4:intron:Veph1 4:intergenic:Olfr481-Olfr482 4:intergenic:Gm6632-Gm22308 4:intron:4930422G04Rik 4:intergenic:1600002D24Rik-Psmg1 4:intergenic:Gm22827-Gm22181 3:intron:Cep192 4:intergenic:Gm23980-Gm22810 4:intron:Osbpl1a 4:intergenic:Gm22119-Gm6026 4:intron:Fat3 3:intergenic:Gm23355-Gm21833 4:intergenic:Minpp1-Papss2 4:intergenic:Mut-Gm6771 4:intergenic:Gm5560-Naa11 4:intron:1700112E06Rik 4:intron:Nol4 3:intergenic:Gm9800-Fhit 2:intergenic:Gm23437-Gm24818 4:intergenic:Adamts5-Gm25715 4:intergenic:1700001G01Rik-Gm26235 4:intergenic:Clrn1-Gm22491 4:intergenic:Cnksr3-Ulbp1 4:intergenic:Gm25096-Gm25634 4:intron:Sema3c 4:intergenic:mmu-let-7j-Gm6214 4:intergenic:Tbc1d21-4930461G14Rik 4:intergenic:Gm7729-Gm24246 3:intron:Cntn4 3:intergenic:2410018M08Rik-Nupr1l 4:intron:Grik1 4:intergenic:Olfr47-Arhgef5 4:intergenic:Gm13349-Dnajc1 4:intergenic:Cog5-Gpr22 4:intergenic:Gm26662-Kirrel3 3:intergenic:Gm23273-9330158H04Rik 2:intergenic:Gm22438-Gm22159 4:intergenic:Mcpt4-Mcpt8 4:intergenic:Igsf11-Gm24795 4:intergenic:Gm24246-Pik3c3 2:intron:Tgfbr2 4:intergenic:Ttc32-Gm7099 4:intergenic:Gm22945-Gm25522 4:intergenic:1700121N20Rik-Gm3191 4:intergenic:Btg1-Gm24587 4:intergenic:Cd83-Gm23117 4:exon:Olfr143 4:intergenic:Gm15975-Gm23896 4:intergenic:1700123L14Rik-Gm22971 4:intergenic:mmu-mir-6343-Gm25974 4:intron:Auts2 4:intergenic:Gm24774-Pcdh17 4:intergenic:Gm23061-Ostf1 3:intron:Skint6 4:intergenic:Gm3833-Fhit 4:intergenic:B930078G14Rik-Fam155a 3:intergenic:Skint2-Gm12814 4:intergenic:Gm9578-Gm22891 4:intron:Sgk3 4:intergenic:Gm23777-Slc16a7 4:intergenic:Gm5161-Ephb1 4:intergenic:A530053G22Rik-Snca 4:intergenic:6720401G13Rik-2610018G03Rik 4:intron:Anks1b 4:intergenic:Gm4845-Gm25457 4:intergenic:Zfp353-ps-Gm6180 4:intron:Otogl 4:intergenic:Gm22966-Gm15443 4:intron:Arf2 4:intergenic:Gm25574-Ankrd50 4:intergenic:Fgf10-Nnt 3:intergenic:Gm23777-Slc16a7 1:intergenic:Gm24121-Ugt2b35 4:intergenic:Luzp2-Gm26482 4:intron:Slc9a9 4:intergenic:Gm25269-Ush2a 4:intergenic:Gm26171-Fam49a 4:intergenic:4930564D02Rik-1700095B22Rik 4:intron:Trerf1 4:intergenic:Gm14700-Gm14705 4:intergenic:Gm25578-Actr3 4:intergenic:Shroom4-Dgkk 4:intergenic:Snx7-Gm26871 4:intergenic:Gm20516-Gm21947 4:intron:A230006K03Rik 4:intergenic:Mapre2-Zfp397 4:intergenic:9530051G07Rik-Gm5396 4:intron:Il16 4:intergenic:Exoc5/Ap5m1-Ap5m1 4:intergenic:Fsip2-Gm13680 4:intergenic:Gm13598-Gm13599 3:intergenic:Gm5776-Luzp2 4:intergenic:Gm5096-Gm24987 4:intergenic:Gm14002-Slc12a1 4:intergenic:1110059M19Rik-Gm14606 4:intergenic:Tbc1d22a-Gm23416 4:exon:Mapk10 4:intergenic:5S_rRNA-Gcnt4 4:intron:Fam19a2 4:intergenic:Gm26006-Gm26139 4:intron:Lrp1b 4:intergenic:Pdcd1-Gm23389 4:intron:BC052040 4:intron:Pvrl4 4:intron:Sox6 4:intron:Gm20548 4:intergenic:Gm7488-Tbl1xr1 4:intergenic:Gm15405-Gm27039 3:intergenic:Gm5594-Vmn2r-ps59 4:intron:Nek1 2:intergenic:Gm8214-Dusp10 4:intron:Nf2 4:intron:Evc2 4:intergenic:Gm12004-4930554G24Rik 4:intron:Iqub 4:intergenic:Gm11409-Gm11407 4:intergenic:Gm8988-Gm23095 4:intergenic:Gm11746-Slc24a3 3:intergenic:Gm14621-Hs6st2 4:intergenic:Pcdhb5-Pcdhb6 4:intergenic:Gm13754-Gm13749 4:intron:Asb9 4:intron:Lrp1b 4:intergenic:Ror2-Sptlc1 4:intron:Ghr 4:intron:Ppp1r13b 4:intron:Ncor1 4:intron:Fam163b 3:intergenic:Cyp2c69-Cyp2c37 4:intergenic:Gm26345-Gm10566 4:intergenic:Gm14979-Gm22552 4:intergenic:Gm23947-Foxn2 3:intergenic:Fbxo8-Hand2 4:intron:Gm12830 4:exon:Olfr894 4:intron:Pcdh9 4:intron:Pcgf3 4:intergenic:Gm9843-Usp25 4:intron:Stk32a 4:intergenic:Gm22828-Gm23960 4:intergenic:Gm26250-Gm11819 4:intron:Antxr2 4:intergenic:Gm23242-Gm25670 3:intergenic:Gm24212-Tox3 3:intergenic:Gm6433-Gm16035 4:intergenic:Gm5391-Gm6806 3:intergenic:AC166333.1-Scg2 4:intergenic:Rpl35a-ps4-Gm13910 4:intergenic:Gm26963-Ypel5 4:intergenic:Gm13798-Gm13797 4:intergenic:Pabpc4l-mmu-mir-6379 4:intergenic:Gm15985-Gm6116 4:intergenic:Gm23537-Gm25003 4:intergenic:Gm25985-Gm6404 4:intergenic:Enox1-Gm10132 4:intergenic:Gm25976-Acot10 3:intergenic:Gm15975-Gm23896 4:intergenic:Gm26164-Gm10283 4:intron:B230110C06Rik 4:intergenic:Gm13803-Gm13805 4:intergenic:Gm22163-Robo2 4:intergenic:Gm13498-Rnd3 4:intron:Atg5 3:intergenic:Gm25702-Gm5473 3:intergenic:Htr1a-Dph3b-ps 4:intergenic:Pcdh20-Gm25886 4:intergenic:Gm26256-n-R5s61 4:intergenic:Frk-Gm26341 4:intergenic:Gm24985-Vwde 4:intergenic:1700061I17Rik-D3Bwg0562e/Gm22408 4:intergenic:Klf10-Gm26863 4:intron:Pde4d 4:intergenic:Prss35-Snap91 4:intergenic:Colec10-Mal2 4:intron:Cpvl 4:intergenic:Gm21614-Gm23249 3:intergenic:Gm24733-Gm807 2:intergenic:B230206H07Rik-Gm25725 3:intron:Gpc6 3:intron:Lrp1b 3:intron:Hdac1 3:intergenic:Bank1-Ppp3ca 4:intergenic:Gm26069-Gm25811 4:intron:Nav3 4:intergenic:Llph-ps2-Gm16481 4:intergenic:Tcea1-ps1-Kif21a 4:intron:Lrp1b 4:intron:Gen1 4:intron:Nphs1 4:intron:Egflam 4:intergenic:Gm22913-Gm20091 4:intron:Usp32 4:intron:Mcf2 4:intron:Pard3b 4:intergenic:Gm23434-Cntnap5b 4:intergenic:Ttc6-Sstr1 4:intron:Slc25a12 4:intergenic:Dock10-Nyap2 4:intergenic:Eci2-Gm22674 4:intergenic:Cntnap4-Gm25766 4:intergenic:4930480K15Rik-Nrxn1 4:intergenic:Gm22715-Gm24602 4:intron:Grxcr1 4:intergenic:Gm22016-Cdc73 4:intron:Ctnnal1 4:intergenic:Rmnd5a-Rnf103 4:intergenic:Syk-Auh 4:intergenic:Slc35f1-Cep85l 4:intergenic:Gm25144-Gm23147 4:intron:Cdh13 4:intron:Sh3yl1 4:intergenic:Arl15-Ndufs4 4:intergenic:RP24-364K9.2-Gm25250 4:intergenic:Htr1a-Dph3b-ps 4:intergenic:Gm20514-Gm20515 4:intergenic:Gm25051-Gm1818 4:intergenic:Gm5627-Ryk 4:intron:Ryr2 4:intergenic:Cbln2-Gm5096 4:intergenic:Fam84a-Gm9847 4:intron:Diap3 4:intergenic:Gm5387-Mbnl3 4:intergenic:Gm24295-Mef2c 4:exon:Ddx59 4:intergenic:Pias2-Gm7276/St8sia5 4:exon:Cnga1 4:intron:Map1b 4:intergenic:Gm12960-Spata6 4:intergenic:Slit3-Gm12123 Off-target primers show all... show less...
553 / rev	`ATAATCTACCATATTTCTCC TGG` `.................... ...` Enzymes: XcmI, StyD4I, LpnPI, BstNI, MaeI Cloning / PCR primers	66	82	50	-3	68	76	0 - 0 - 5 - 29 - 148 0 - 0 - 2 - 5 - 4 182 off-targets	4:intergenic:Fam170a-Hdhd1a 3:exon:BC051070 4:intergenic:Gm24288-Gm17215 3:intergenic:Slc9a1-Gm13257 4:intron:Cntn4 4:intergenic:Gm5185-Gm24921 4:exon:Prmt10 4:intron:Epn2 4:intergenic:Macrod2-Gm25583 4:intergenic:Ces1c-Ces1d 4:intergenic:Gm23306-Gm4758 3:intergenic:Gm14657-Gm8260 4:intron:Ces1b 4:intergenic:Diap2-Gm14987 2:intergenic:Gm25633-Gm8907 4:intergenic:Gm24691-Grm8 4:intron:Cbl 4:intron:Arhgap26 3:intergenic:1500009L16Rik-Nuak1 4:intergenic:Gm23117-Gm5083 4:intron:Lrch1 4:intergenic:Lamr1-ps1-Prnp/PRND 3:intergenic:Gm5621-Pik3r4 4:intergenic:Thpo-Chrd 4:intergenic:Slco6c1-D1Ertd622e 4:intergenic:Hivep2-Gm24343 4:intergenic:4930578C19Rik-Gm6923 4:intron:Gm11190 4:intergenic:Slc9a7-Gm9083 4:intron:Gtf2e1 4:intron:Npr3 4:intron:Ddx60 4:intergenic:Gm14883-4930578C19Rik 3:intergenic:Dmd-n-R5s9 4:intron:Morc1 4:intergenic:Gm26901-Sntg1 4:intergenic:Gm9025-Gm15322 3:intron:Ces1c 4:intron:Etv1 4:intergenic:Gm20713-Gpc5 4:intergenic:Gm26307-Gm26937 4:intron:Hmgcll1 4:intron:Prkca 4:intergenic:Gm26307-Gm26937 4:intergenic:Sep15-Sh3glb1 4:intron:Adamtsl1 3:intron:Alg13 4:intergenic:Gm15663-Mettl25 4:intron:Msi2 4:intergenic:Snx7-Gm26871 4:intron:F5 4:intergenic:Ppp2r3d-Gm20783 4:intron:Ptpn13 4:intergenic:Gm11634-Gm26316 4:intergenic:Gm23629-Ctsb 3:intergenic:Schip1-Il12a 2:intron:4930440I19Rik 3:intergenic:Gm833-Gm25779 2:intron:Bzw1 4:intergenic:n-R5s194-Gm23937 4:intergenic:Gm22321-Gm1401 4:intergenic:Gm11445-Gm11446 4:intergenic:Gm22114-1700084C01Rik 4:intergenic:Pcdhb14-Pcdhb15 4:intergenic:Gm24591-Gm26241 4:intergenic:Gm24968-Gm22797 4:intergenic:Gap-CAAA01205117.2 4:intergenic:Gm17192-Gm24164 4:intergenic:Gm24839-Tmem132c 4:intergenic:Wdfy3-Arhgap24 4:intergenic:Gm24398-Xpo1 4:intergenic:Dusp10-Gm23349 4:intergenic:Gm12804-Gm12805 4:exon:Yars2 3:intergenic:Kat6a-Ank1 4:intergenic:Gm11403-Hmgb1-rs18 4:exon:March1 4:intergenic:Gm12392-Cnbd1 4:intergenic:Fbxo3-Gm24644 4:intergenic:Gm25509-n-R5-8s1 4:intergenic:Gm6984-Htr2a 4:intergenic:Gm15384-Rab39b/Gm15063 4:intergenic:Gm5899-Gm23884 4:intergenic:Psma8-Taf4b 3:intergenic:Gm26977-Pard6g 4:intergenic:Kcnh8-Efhb 4:intergenic:Gm25636-Ipo7 4:intron:Cabin1 4:intergenic:Gm26164-Gm10283 3:intergenic:Robo2-Gm26307 4:intergenic:Ankef1-Snap25 4:intergenic:Grm8-Gm26310 4:intergenic:Apcs-Gm26077 4:intron:Spidr 4:intron:Dpp6 4:exon:Alkbh1 4:intergenic:Gm5124/Gm26593-Agtr2 2:intergenic:Gm14070-Tasp1 4:intergenic:Gm24633-Gm25568 4:intergenic:A1bg-Gm24696 4:intergenic:Gm14975-Gm4916 4:intergenic:Gm22332-Gm14701 4:intron:Zmat4 4:intergenic:Ppapdc1a-Wdr11 4:intron:Ankhd1 3:intergenic:Ins1-Xpnpep1 4:intergenic:Il1rapl2-Gm14988 4:intergenic:Gm12914-Gm11264 4:intergenic:Mir1264-Mir1298 4:intergenic:Gm23538-1700110M21Rik 4:intergenic:Gm23154-Gm12604 4:intron:Ptn 4:intergenic:Rnf144b-4931429P17Rik 3:intergenic:Kcnv1-Gm5472 4:intergenic:Smad3-Smad6 4:intergenic:Hdhd1a-Prr16 4:intergenic:4930445B16Rik-Gm17075 4:intergenic:Gm22296-Gm23799 4:intergenic:Gm22825-Zfhx3 4:intron:Pdzrn4 4:intergenic:Gm23203-Gm23447 3:intergenic:Igkv1-115-Igkv2-112 3:exon:Eif3a 4:intergenic:n-R5s218-Pla2g4a 3:intergenic:9930014A18Rik-A1bg 4:intron:Wdr43 3:intergenic:Gm7910-Khdrbs2 3:intergenic:5730559C18Rik-Gpr25/Gm26781 3:intergenic:Gm25497-Lrrn3 3:intergenic:Gsdmd-Mroh6 4:intergenic:Prune2-Gcnt1 4:intergenic:4930412B13Rik-Gm13986 4:intron:Map1b 4:intron:Mgat4c 4:intergenic:Klra10-Klra13-ps 4:intergenic:Gm24246-Pik3c3 3:intergenic:Gm23139-Dab2 3:intergenic:Gm23752-Gm24815 4:intron:Ifitm10 4:intergenic:Csmd1-Gm25665 4:intergenic:Gm25509-n-R5-8s1 4:intron:Smim12 2:exon:4933425L06Rik 3:intron:Tead1 3:intergenic:Tas2r116-Tas2r110 4:intergenic:Gm13662/Gm13663/Gm17250-Gm13662 4:intron:Cadm1 4:intergenic:RP23-13G1.6-Hmgn3 3:intergenic:Gm25015-Gm7481 4:intron:Ptpn22 4:intergenic:Gm10256-Gm10352 4:intergenic:Gm21693-Gm21704 4:intergenic:Gm21704-Gm3376 4:intergenic:Gm16501-Rbmy/Gm10256 4:intergenic:Gm21677-Gm21693 4:intergenic:Gm21708-Gm21704 4:intergenic:Rbmy-Gm10256 4:intergenic:Gm10352-Gm21677 4:intergenic:Rbmy/Gm10256-Rbmy 4:intergenic:Gm21704-Gm21708 3:intergenic:Il1rapl2-Gm25393 4:intron:Xlr4c 4:intron:Xlr4a 4:intergenic:Gm7808-Olfr866 4:intergenic:Gm14888-4930550L24Rik 4:intergenic:Gm20514-Gm20515 4:intergenic:Pde1c-Gm24709 4:intron:Adamts4 4:intergenic:Steap4-Gm15731 3:intergenic:Otud6b-Tmem55a 4:intron:Hpse2 4:intergenic:Mfap3l-Gm23329 4:intergenic:Zeb2-VIS1 4:intergenic:Gm23752-Gm24815 4:intergenic:Gm22031-Gm4823 4:intron:Mecp2 4:intron:Itpkc 4:intergenic:E130304I02Rik/Zfp507-Gm10166 4:intron:Lama2 4:intergenic:Zmiz1/Zmiz1os1-Zmiz1 4:intergenic:Cd109-Gm8116 4:intergenic:Pros1-Epha3 Off-target primers show all... show less...
86 / fw	`CTTTTCTGCCATGATTGCTC AGG` `.................... ...` Not with U6/U3 Enzymes: BstDEI, MwoI, Hpy188III, BspCNI Cloning / PCR primers	65	78	39	28	81	83	0 - 0 - 3 - 38 - 269 0 - 0 - 0 - 1 - 2 310 off-targets	4:exon:2610204G22Rik 4:exon:Traf1 4:exon:1700122E12Rik 4:intergenic:Dfna5-Osbpl3 4:intergenic:Gm10192-Gm12696 4:intergenic:Gm7191-Gm23494 3:intergenic:BC042782-Gm12708 4:intergenic:Zkscan2-Gm23788 4:intergenic:Gm23378-Gm22266 2:intergenic:Samd5-Stxbp5 4:intergenic:Mir3963-Eltd1 4:intergenic:Gm21746-Gm20873 4:intron:Dock4 4:intergenic:Gm12010-Gm12011 4:intergenic:Pdgfc-Gm22531 4:intergenic:Gm24295-Mef2c 3:intergenic:Pard3b-4930448K12Rik/Pard3b 4:intergenic:Plekhs1-Dclre1a 4:intergenic:Sh3tc2-Adrb2 4:intergenic:Sema5b-Dirc2 4:intron:Ptprt 4:intergenic:Gm15422-Gm17615 4:intergenic:Cypt2-Gm14823 4:intergenic:Gm25714-Gm24997 3:intergenic:Gm21614-Gm23249 4:intron:Ksr2 4:intergenic:Lrrc8d/D830014E11Rik-Gm10359 4:intron:Sptb 4:intergenic:Gucy1a2-Gm22195 4:intergenic:Gm11427-Gm23444 4:intergenic:AC175117.1-Hnf4g 4:intergenic:Gm3242-Gm17047 4:intron:Gm13912 4:intergenic:Agbl1-Gm23239 4:intergenic:Gm22693-C230029F24Rik 4:intron:Cadps 4:intron:Fstl5 3:intron:Adam39 4:intergenic:Gm10371-Gch1 4:exon:Kalrn 3:intergenic:Gm5742-Gm22223 4:intergenic:Gm12084-Pnpt1 3:intron:Med12l 4:intron:Zcchc7 4:intergenic:4933407K13Rik-Gm7153 3:intron:Sox5 4:intergenic:Rpl31-ps25-AC135509.1 3:intergenic:Gm4906-Gm14515 4:intergenic:Gm26396-Gm13795 4:intergenic:Gm23337-Gm26081 4:intergenic:Slc7a11-Gm24503 3:intergenic:Gm13368-Gm13364 4:intergenic:Mro-Mapk4 3:intron:Yae1d1 4:intergenic:Rgs5-Rgs4 4:intergenic:Mir1931-Cdk17 4:intron:Dgkb 4:intron:Cadm2 4:intergenic:Gm16290-Gm17175/Gm7247 4:intergenic:Gm25778-Ppapdc1a 4:intergenic:Gm27000/Gm26958/Mir1906-2/MEG3_2-Gm5759 4:intergenic:Gm12507-Klf4 4:intergenic:Slco6c1-D1Ertd622e 4:intergenic:Gm11262-Gm12913 4:intergenic:Plcl1-Hsfy2 3:intron:Vrk1 4:intron:Moxd1 4:intergenic:Gm13529-9430024E24Rik 4:intron:Htt 3:intergenic:Gm5083-Gm27007 4:intergenic:Ngly1-Top2b 4:intergenic:Mir3072/Mirg-Dio3os 3:intergenic:Mir690-Mb21d2 4:intron:Rimbp2 3:intergenic:Gm2373-5330416C01Rik 4:intergenic:Gm9992-Gm25872 4:intron:Rorb 4:intron:Tmco3 4:intergenic:Prkcb-Gm14389 4:intergenic:AC154801.1-BC040587 4:intron:Tbc1d7 3:intron:Arid1a 4:intergenic:En1-B230209K01Rik 4:intergenic:Rpl27a-ps2-Slitrk1 4:intergenic:Gm5546-Lrig2 4:intergenic:Ddx60-Anxa10 4:intergenic:Gm13579-Gm13581 4:intron:Gm12877 4:intron:4732456N10Rik 4:intron:Gng2 4:intron:Agl 4:intron:Arid1b 4:intergenic:Gm25103-Dph6 4:intron:Ryr3 3:intergenic:Gm13481-Gm13471 4:intron:Slc8a1 4:intergenic:Gm25958-Gm16294 4:intron:Trim66 4:intron:Mob2 4:intron:Exoc6b 4:intron:Casc1 4:intergenic:Gm24254-4933436C20Rik 4:intron:Lphn3 4:intergenic:Rpsa-ps10-Mir3963 4:intron:Nxpe3 4:intergenic:Krcc1-Gm1070 4:exon:Ppp4c 3:intergenic:Stk24-Slc15a1 4:exon:Trak2 4:intergenic:Krtap20-2-Krtap21-1 4:intergenic:Olfr811-Olfr812 4:intergenic:Gm14982-Gm26209 4:intergenic:Gm7191-Gm23494 3:exon:Oas2 3:intergenic:Oaz1-ps-Riok2 4:intron:Cacna1e 4:intergenic:Gm9621-Gm24200 4:intergenic:Dclk3-Stac 4:intergenic:Rpl30-ps11-Atp6ap2 4:intergenic:Cbln2-Gm5096 4:intergenic:Gm5292-Fgfbp1 3:intergenic:Rnpepl1-Capn10 4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Gm13506-Gm13454 4:exon:Col6a3 4:intergenic:Csmd1-Gm25665 4:intron:Kif16b 4:exon:C330007P06Rik 4:intergenic:Gm13483-Gm13488 4:intergenic:Gm24910-Gm25784 4:exon:Gm5398 4:intergenic:Taar4-Taar5 4:intron:Slc25a53 4:intergenic:BC048507-Mtrr 4:intron:Rogdi 3:intergenic:Gm26291-Gm17377 4:intergenic:Dab1-Gm12719 4:intergenic:1700003M02Rik-Gm136 3:intron:Foxn1 4:intron:Jakmip3 3:intergenic:Gm25702-Gm5473 3:intergenic:Gm21846-6030443J06Rik 4:intron:Fbln7 4:intergenic:Cdh7-Cdh19 4:exon:Zbtb20/Mir568 4:intergenic:Immt-Ptcd3 4:intergenic:AC112986.1-Tsn 4:intron:Creb1 4:intergenic:Bbs10-Nap1l1 3:intergenic:Mcph1-Agpat5 4:intron:Tgfbr3 4:intergenic:Aff3-Lonrf2 3:intergenic:Gm25602-Tbc1d32 4:intergenic:AI314180-Gm26400 4:intergenic:Rpl31-ps18-Ins1 4:intergenic:Macrod2-Gm14078 4:intron:Dock7 4:intron:Neil3 4:intergenic:Otoa-Hs3st2 4:intergenic:Gm14465-Itga4 4:intergenic:mmu-let-7j-Gm6214 4:intergenic:Gm11779-Gm11782 4:intron:Trp53inp2 4:intergenic:Gm14835-4933401B06Rik 4:intergenic:Gm24462-C230034O21Rik 4:intron:Gli2 4:intergenic:Tlr12-Gm12958 4:intron:Fam13c 4:intergenic:Gm10220-Gm7361 4:intron:Gng12 4:intergenic:5033428I22Rik-Scrg1 4:intergenic:n-R5s42-Fam19a5 2:intergenic:Gm20475-Slit2 4:intron:Cmip 4:intron:Opcml 3:intergenic:Gja1-Gm9795 4:intron:Wwp1 4:intergenic:4930578C19Rik-Gm6923 4:intergenic:Gm3693-Slc8a3 3:intergenic:Gm24720-Tshz1 4:intergenic:Kcnk13-Psmc1 4:intron:Eva1c 4:intergenic:Gm25309-Pdlim3 4:intergenic:4933415F23Rik-Gm24176 4:intron:Tpte 2:intergenic:Gm14621-Hs6st2 4:intergenic:Nkd1-Snx20 4:intergenic:Gm22802-Slitrk5 4:intron:Cyb5rl 4:intergenic:Lmod1-Gm22796 4:intergenic:Gm26559-Gm24471 4:intron:Oasl2 4:intergenic:Gm8840-Ikzf2 4:exon:Trio 4:intergenic:Gm24795-Gm8609 4:intron:Myo1e 4:intron:4930533I22Rik 3:intron:Skor2 4:intron:Gabra4 4:intergenic:Gm12632-Gm25244 4:intergenic:Gm14885-BC049702 4:intergenic:Gm12261-Olfr223 3:intergenic:Gm15975-Gm23896 4:intergenic:Gm26468-Rpsa-ps10 4:intergenic:Dclk3-Stac 4:intergenic:Pcdh8-Gm6999 3:intergenic:Nbea-Mab21l1 4:intergenic:Ccnl1-Veph1 4:intron:Dlgap3 4:intergenic:Zfp516-Gm24720 3:intron:Polh 4:exon:Olfr1357 4:intron:Slc47a2 4:intergenic:Gm22242-Ddx10 4:intergenic:Gm25247-Tshz3 4:intergenic:AC132241.1-Gm9898 4:intergenic:Serpina12-Serpina4-ps1 3:intergenic:Gm23822-4921504A21Rik 4:intergenic:Fam198b-Gm6706 4:intron:Bai3 4:intergenic:Birc3-Yap1 4:intergenic:Gm24256-Grik2 4:intergenic:Large-Gm23255 4:intergenic:Plod2-Gm9621 3:intergenic:Pvrl3-Gm6912 4:intergenic:C630043F03Rik-Gm11235 4:intergenic:Gm25843-Tmeff2 4:intergenic:Gm8919-Gm8935 4:intron:Cask 4:intergenic:Gm13809-Gm13808 4:intergenic:Gm23892-Gm25382 4:intron:Chchd3 4:intergenic:Ccser1-Gm22212 4:intergenic:Gm9308-Itgbl1 4:intron:Gm13335 4:intergenic:Gm5764-Gm15185 4:intergenic:Sval3-Sva 4:intergenic:Tcstv1-Gm21761 4:intergenic:Kcnf1-Pdia6 4:intergenic:Kcnf1-Pdia6 4:intergenic:Snap25-Mkks 4:intergenic:Agpat6-Gins4 4:intergenic:Nbas-Gm4929 4:intergenic:Dtd1-Scp2d1 4:intergenic:Malt1-Zfp532 4:intergenic:Gm14534-5730405O15Rik 4:intergenic:BC147527-Tcstv3 4:intron:C1qb 4:intergenic:Gm20767-Gm21818 4:intergenic:AF067061-Gm20900 4:intergenic:Gm5185-Gm24921 4:intron:Tmem232 4:intron:Zfp874b 4:intergenic:Ap5m1-Naa30 4:intergenic:Gpr139-Gm23321 4:intergenic:Bckdhb-Gm26146 4:exon:Gm13134 4:intron:Nphp4 4:intergenic:Gm14481-Gm14483 4:intron:Myt1l 4:intergenic:AC166813.1-2610316D01Rik 4:intergenic:4933407I08Rik/Ppp2r2b-Ppp2r2b 4:exon:Txnrd2 4:intron:Glrx3 3:intron:Gm13853 4:intergenic:Col11a1-Gm23750 4:intergenic:Pax2-Fam178a 4:intergenic:D930007P13Rik-Smug1 3:intergenic:Sox13-Gm23939 4:intergenic:Spata18-Usp46 4:intergenic:AC102738.1-St6galnac3 4:intergenic:Epm2a-Gm16207 4:intron:Vwa3b 4:intron:Gpr39 4:intergenic:Hk2-Sema4f 4:intergenic:Gm24066-Gm6395 4:intergenic:Pcdh18-Slc7a11 3:intron:Slco5a1 4:intergenic:Gm5356-Gm26331 4:intergenic:Gm10638/Siah1a-N4bp1 4:intergenic:Mir5133-Gm25603 4:intron:Apba1 4:intergenic:Sdr42e1-Hsd17b2 4:intron:Ccbl1 4:intergenic:Gm11986-U7 4:intergenic:Tomm5-Gm12566 4:intergenic:Lamc1-E330020D12Rik 4:intergenic:Gm23225-Gm24127 4:intergenic:Gm23180-Gm22977 4:intergenic:Gm12771-Scgb2b22-ps 4:intergenic:Gm9956-Gm24285 4:intergenic:Gm13731-Olfr1120 4:intron:Cdc42bpg 4:intron:Scarf2 4:intergenic:Fgfr4-Nsd1 4:intergenic:Fbxo15-Gm25453 4:intron:Fam172a 4:intron:Npas3 3:intron:Gys1 4:intergenic:Ube2nl-Gm25533 4:intron:Map3k2 4:intergenic:Gm23987-Fam135b 4:intergenic:Gm8216-Mir3472 4:intergenic:Pou2f3-rs1-Serpinb12 4:intron:Cntnap5c 4:intergenic:Pcdh17-Gm23926 4:intergenic:Gm24350-Gm13464 4:intergenic:Gm10205-4930435H24Rik 4:intron:Lipc 4:intergenic:Gm9341-Gm24444 Off-target primers show all... show less...
222 / fw	`TTATGTTTGCACTAATCTGT GGG` `.................... ...` Enzymes: Csp6I Cloning / PCR primers	65	81	58	28	71	81	0 - 1 - 5 - 13 - 171 0 - 0 - 2 - 0 - 5 190 off-targets	4:intron:Tspan2 4:intergenic:Gm2056-Gm16368 4:intergenic:BC030500-Gm24847 4:intergenic:Gm22382-Gm22169 4:intergenic:Gm22852-Spt1 4:intergenic:Gm25715-N6amt1 4:intergenic:Dlg2-Gm23928 4:intron:Gm15506 4:intron:Ptbp2 4:intergenic:Gm15971-Dyrk1a 4:intergenic:5430403G16Rik-4930522L14Rik 4:intergenic:Irgm2-4930438A08Rik 4:intergenic:Dnajc25-Gng10 4:intergenic:Il1rn-Psd4 4:intron:Cyp2c44 4:intergenic:Gm22136-Gm22206 4:intron:Ell2 4:intergenic:Gm22527-Rnf144a 4:intergenic:Slc35f1-Gm25664 3:intergenic:mmu-mir-6354-Gm5265 4:intergenic:Prdx6-ps2-Gm11897 4:intergenic:Gm22713-Prss12 4:intergenic:Impg1-RP24-325L16.1 4:intergenic:4930511F01Rik-Snrpb2 4:intergenic:Olfr192-Olfr193 4:intron:Cpped1 4:intergenic:Gm11889-Gm25978 4:intergenic:Gm10428/Zfp286-4921522H14Rik 4:intergenic:Bambi-Gm23643 2:intergenic:Nox3-Gm23186 4:intron:Frmpd4 4:intergenic:Gm24704-Gm24062 4:intergenic:Gm24935-Gm21232 4:intergenic:Gm5614-Cdon 4:intron:Tmc2 4:intergenic:Fgf10-Nnt 4:exon:H3f3a/Gm17275 2:intergenic:Gm7416-Gm7146 4:intergenic:Gm25176-Smarca2 4:intergenic:Veph1-Shox2 4:intron:Chi3l7 4:intergenic:Gm5529-Gm23934 4:intergenic:Tmem126b-Dlg2 4:intergenic:Mc2r-mmu-mir-6356 4:intron:Scn7a 3:intergenic:Neurod6-Ccdc129 3:intergenic:Gm25247-Tshz3 4:intergenic:Gm15596-Cftr 4:intergenic:Kcnb2-Gm25227 4:intron:Utrn 4:intron:Cdk14 4:intergenic:Gm15394-Gm24547 3:intron:Fam19a5 4:intergenic:Gm25656-Cntn6 4:intergenic:Gm13221-Gm13219 4:intergenic:Sema6a-Gm5506 4:intron:Eml6 4:intergenic:Gm26263-Pax3 4:intergenic:Gm11510-Msi2 4:intergenic:Gm23022-Rab28 4:intergenic:Gm14406-Gm14324 4:intergenic:Gm14409-Gm14412 4:intergenic:Gm14322-Gm14325 4:intergenic:Gm14412-Gm14418 4:intergenic:Vmn1r79-Vmn1r80 4:intron:Gm11867 4:intergenic:Rpsa-ps10-Mir3963 4:intergenic:Slitrk1-Gm25901 4:intergenic:Gm13481-Gm13489 4:intron:Gm1305 4:intergenic:Slc33a1-Slc33a1/Gm26850 4:intron:Adamts6 4:intergenic:Erbb4-Gm8840 4:intron:Lrrc19 4:intergenic:Gm24680-4921530L21Rik 4:intergenic:Ctnna3-Gm10118 4:intron:Tnni3k 4:intergenic:Olfr1451-Olfr1452-ps1 4:intergenic:Immp2l-Gm25248 4:intergenic:Gm16227-A930011G23Rik 4:intron:Arid5b 4:intron:Xpo7 4:intergenic:Celf2-Gm13210 4:intergenic:Arfgef1-Cpa6 4:intron:Syne1 4:intergenic:Gm15394-Gm24547 4:intron:Ylpm1 4:intron:Camsap1 4:intergenic:Gm25578-Actr3 4:intergenic:Mut-Gm6771 4:intron:Ccdc7 4:intergenic:Igkv9-120-Igkv1-117 4:intron:AU020206 4:intergenic:Sprr2e-Sprr2f 2:exon:Ncoa3/Sulf2 4:intergenic:Fshr-Gm10184 4:intergenic:Mfhas1-Gm22216 4:intron:Acsm1 4:intergenic:Sema3d-Gm23243 4:intergenic:Gm5294-Pdgfa 2:intron:Sema5a 4:exon:Ncf2 4:intergenic:Bmper-Npsr1 3:intergenic:Gm1966-Olfr693 4:intergenic:Epha3-Csnka2ip 4:intergenic:Emc2-Tmem74 4:intergenic:F930017D23Rik-Gm9034 4:intron:Ccdc141 4:intron:Lpp 4:intron:Edil3 4:intergenic:Dpp10-Gm10543/Dpp10 4:intergenic:Mir802-Setd4 4:intergenic:Sprr2e-Sprr2f 2:intron:Gzma 4:intergenic:Gm26119-Fzd8 4:intergenic:Arhgap28-L3mbtl4 4:intergenic:Gm7571-E330012B07Rik 4:intergenic:Gm21614-Gm23249 4:intron:MDGA2/Mdga2 4:intergenic:Ttll7-Gm23131 4:intergenic:Gm23987-Fam135b 4:intergenic:4930544I03Rik-n-R5s65 4:intergenic:Gm8601-Btg1 4:intron:Mllt3 4:intergenic:Gm23011-Gm26691 4:intron:Tekt5 3:intergenic:Ppargc1a-mmu-mir-6417 3:intergenic:Gm22331-Gm24937 4:intergenic:Cdh19-Dsel 4:intron:4933402N03Rik 4:intergenic:Gm14981-Gm26406 3:intron:Sh2b3 4:intron:Mcf2 4:intergenic:Gm11803-Gm11801 4:intergenic:Rpe65-Wls 4:intergenic:Gm4487-Gm24073 4:intergenic:Gm24467-Gm13456 4:intergenic:Gm16863-Vps8 4:intron:Fmnl2 4:exon:Faxc 4:intergenic:Gm22658-Vstm5 4:intron:Srebf2 4:intergenic:Isoc1-Adamts19 4:intergenic:Gm8730-Cdh5 3:intergenic:Gata6-Rbbp8 4:intergenic:Gm25714-Gm24997 4:intergenic:Ccl11-Ccl12 4:intergenic:Ptpn4-Tmem177 4:intron:Zpbp 4:intergenic:Hspa4-Zcchc10 3:intergenic:Gm6931-Ift57 4:intron:Magi2 4:intron:Kif16b 4:intergenic:AC121988.1-Gm25740 4:intron:Rbak 4:intron:Mef2a 4:intron:Scube1 4:intron:Akap9 3:intergenic:Gm23752-Gm24815 4:intron:Boll 4:intergenic:Gm23189-Gm15671 4:intergenic:Alcam-Gm25723 1:intron:Rb1 4:intergenic:Cdk15-Fzd7 4:exon:Srpk2 4:intron:Prlr 4:intergenic:Gm9843-Usp25 4:intergenic:Vmn1r35-Vmn1r36 4:intron:Klhdc2 4:exon:Cdk14 4:intergenic:Gm22945-Gm25522 4:intergenic:Gm20698/Vmn2r7/Gm26939-Vmn2r7/Gm26939 4:intergenic:Gm25843-Tmeff2 4:intergenic:Cntn6-Gm23339 4:intergenic:Gm23038-4930570G19Rik 4:intergenic:Gramd1c-Atp6v1a 4:intergenic:Gm9923-Gm24984 4:intergenic:Gm25509-n-R5-8s1 4:intergenic:Cdh19-Dsel 3:intergenic:Gm26341-Amd2 4:intergenic:Ebf1-Gm12159 4:intergenic:Gm23013-Ppp1r3a 4:intergenic:Gm25493-Mrpl15 4:intergenic:AC102738.1-St6galnac3 4:intron:Mib1 4:intergenic:Wif1-Tbc1d30 4:intergenic:Gm5523-Gm25491 3:intergenic:Ptgs1-Olfr334-ps1 4:intergenic:Agbl1-Gm23239 4:exon:4933431K23Rik Off-target primers show all... show less...
116 / rev	`GTATTGCTAAGCCATAAATG TGG` `.................... ...` Enzymes: BstXI, SmiMI Cloning / PCR primers	64	86	57	24	72	85	0 - 1 - 0 - 27 - 90 0 - 1 - 0 - 2 - 5 118 off-targets	3:intron:Tmem255b 3:intergenic:Gm6327-Ercc4 4:intergenic:Gm5257-mmu-mir-6343 3:intergenic:Calcoco1-Hoxc13 4:intron:2810055G20Rik 4:intergenic:Opcml-Gm15606 4:intron:Ptprn2 4:intergenic:Gm23625-Mad2l1 4:intron:Ercc6l2 4:intergenic:Rpl31-ps8-Tcea1-ps1 3:intron:Fam122c 3:intergenic:Astn2-Gm25480 4:intergenic:Gm25708-Gm5262 4:intergenic:Gm24231-Gm24222 3:intergenic:Gm7069-Gm10649 4:intergenic:Gm8793-Nkain2 4:intergenic:Gm24202-Gm8100 4:intergenic:D630028G08Rik-Ctnna3 4:intergenic:Gm6594-Pkdcc 4:intergenic:Gm7931-Slit2 4:intergenic:Pof1b-Gm14922/Gm14923 4:intron:Erbb3 3:intergenic:Agpat9-Gm20484 4:intergenic:Lgals4-Lgals7 4:intergenic:Itpripl2-Coq7 4:intron:Rnf128 4:intergenic:Nrg3-4930529F24Rik 4:intergenic:Galntl6-Gm26905 4:intergenic:Gm9762-Gm23440 4:intergenic:Cald1-Npn2 4:intergenic:Olfr582-Olfr583 3:exon:Rtdr1 4:intron:Gse1 4:intergenic:Olfr1349-Olfr1350 4:intergenic:Gm16260-4930517O19Rik 4:intergenic:Satb1-RP24-465O15.1 4:intron:Bmp7 4:intron:AW554918 4:intergenic:Gm15723-Gm25319 4:intergenic:Gm24812-Gm14774 4:intergenic:Gm10275-Cenpw 4:intergenic:Pdgfc-Gm22531 4:intergenic:Gm23434-Cntnap5b 4:intergenic:Ppp4r2-Pdzrn3 4:intron:5730522E02Rik 3:intergenic:Fam180a-Mtpn 3:intergenic:Samd12-Gm23200 4:intergenic:Gm17333-Gm25025 4:intron:Apbb2 4:intergenic:Gm5455-Gapt 4:intron:Slc36a1 4:intron:Cpeb1 4:intron:Stx17 4:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Gm8526-Edil3 4:intergenic:Rorb-Gm25437 4:intergenic:Gm4487-Gm24073 4:intron:Auts2 4:intron:Egfem1 3:intron:Dock3 4:intergenic:Hcn1-Gm26349 3:intergenic:Gm12010-Gm12011 3:intergenic:Gm16223-Cpeb2/Gm7854 4:intergenic:Igsf21-Gm9867 4:intergenic:Plin2-Dennd4c 4:intergenic:Gm22544-Gm22509 3:intergenic:mmu-mir-6398-Gm12926 4:intergenic:Il21/Gm12534-Gm24169 4:intergenic:Fam194a-Siah2 4:intergenic:Gm25850-Gm23646 4:intergenic:Glrx3-Gm25798 4:intergenic:Gm26468-Rpsa-ps10 4:intron:Fam188b 4:intergenic:Ankrd34c-Mir184 4:intron:Gm12446 4:intron:Stk32b 4:intergenic:9530051G07Rik-Gm5396 3:intergenic:Gm14929-Pabpc5 1:intergenic:Gm12912-Tmem261 4:intergenic:Pld5-Gm26104 4:intron:Wipf1 4:intron:Col5a1 3:intergenic:Gm12912-Tmem261 3:intron:6030443J06Rik 4:intron:Nfasc 3:intron:Ctnna2 4:intergenic:Rps18-ps2-Gm23423 4:intergenic:Gm12650-Gm12651 4:intron:Nell1 3:intergenic:Gm26580/Kcnq5-Rims1 4:intergenic:Gm23029-Gm14937 4:intron:Nfib 4:intergenic:Gm13316-Cacnb2 3:intergenic:Rttn-Cd226 3:intergenic:Gm22011-Mthfr-ps1 4:intergenic:Ccl11-Ccl12 4:intron:Cyp4a31 3:intron:Rab8b 3:intergenic:Gm5161-Ephb1 3:intergenic:Tmem126b-Dlg2 4:intron:Ift140 4:intron:AU019990 3:intergenic:Aard-Slc30a8 4:intron:Rc3h2 4:intergenic:Gm13988-Sema6d 3:intron:4930593A02Rik 4:intergenic:Gm23605-Kctd8 4:intron:Rimbp2 4:intergenic:Gm12377-Gm12376 4:intergenic:Neto1-Cbln2 4:intergenic:Fam3c-Gm8927 4:intergenic:Gm22507-Trhde 4:intergenic:Gm11259-Gm11256 4:intron:Stard6 4:intron:Ptprd 4:intergenic:1700019D03Rik-Mstn 3:intergenic:Gm20732-Gm25987 4:exon:S100a11 Off-target primers show all... show less...
602 / fw	`CATGATTACTCCATATTCTG TGG` `.................... ...` Cloning / PCR primers	64	86	61	31	63	78	0 - 0 - 6 - 35 - 139 0 - 0 - 1 - 2 - 7 180 off-targets	3:intergenic:Gm24745-Lin7a 4:exon:Nqo2/Serpinb6a 4:intergenic:Amd2-Hs3st5 3:intergenic:Gm22529-Gpr37 4:intergenic:Gm5611-Chordc1 4:intergenic:Olfr930-Olfr933 3:intergenic:mmu-mir-6343-Gm25974 4:intron:Ryr2 2:intron:Diap2 3:intergenic:Rbfox1-Gm23476 3:intergenic:Tenm2-Gm12128 4:exon:Dgat1 4:intron:Lama3 3:intergenic:Gm22140-Gm5908 4:intergenic:Nell1-Ano5 4:intron:Fbxl7 4:intergenic:Cyp2c69-Cyp2c37 4:intron:Neo1 4:intron:Slc22a29 4:intron:Gab2 3:intron:Pla2g4d 4:intergenic:Trhde-4930473D10Rik 4:intergenic:Slc22a22-Zhx2 4:intergenic:Tshz3-Zfp536 4:intergenic:Gm24522-4930558G05Rik 4:intron:Wrn 4:intergenic:Gm25068-Ceacam5 3:intergenic:Slc39a11-Gm26052 4:intergenic:Gm10549-2410004N09Rik 3:intergenic:Gm25875-Gm23835 4:intron:Atrnl1 4:intron:Kcnj6 4:intergenic:Rpl31-ps15-Gm26404 4:intergenic:Tab3-Gyk 4:intergenic:Pmvk-Kcnn3 4:intron:Ptprt 4:intergenic:AC102738.1-St6galnac3 4:intron:Pcx 4:intron:Fbxl20 4:intergenic:Gm11997-Vwc2 3:intergenic:Adcy2-Gm26844 4:intergenic:Lnx1-Gm15984 4:intron:1700040L02Rik 4:intergenic:Fam76b-Sesn3 3:intergenic:n-R5s73-Cdk12 3:intron:Nos1ap 4:intron:Pkhd1 4:intergenic:Csmd1-Gm25665 4:intergenic:Fam19a2-Gm23777 4:intron:Prpsap2 4:intergenic:Gm24127-Pcdh9 4:exon:Slc1a3 3:intron:B9d1 4:exon:Itfg1 4:intergenic:Rpl27a-ps2-Slitrk1 4:intergenic:A830092H15Rik-Gm25773 3:intergenic:Gm25485-Gm12460 4:intron:Dnaic1 4:intergenic:3110083C13Rik-Fgf9 3:intergenic:Gm13180-Gm13191 4:intergenic:Gm14659-Gm14657 4:intron:Parpbp 3:intergenic:4930594M17Rik-Gm26012 4:intergenic:Fbxo8-Hand2 4:intergenic:4631405K08Rik-Plxna2 4:intergenic:Gm8183-Tmem135 4:intergenic:Gm9887/Arf6-Gm23018 4:intron:Neil2 4:intergenic:Degs1-Fbxo28 4:intergenic:Smoc2-Thbs2 4:intergenic:Gm9578-Gm22891 3:intron:Gm13849 3:intron:Grin2b 4:intergenic:Pclo-Gm9758 4:intergenic:Rpl7a-ps10-Trim42/Gm15891 4:intergenic:Nxph2-Spopl/Gm13331 4:intron:Elp4 4:intergenic:Olfm4-Gm9578 4:intergenic:Gm20581-Gm20579 4:intergenic:Olfr1437-Olfr1440 4:intergenic:Ccdc54-Gm9575 4:intron:Itpkb 4:intergenic:Gm23847-Fgfr2 4:intergenic:Gm26651-Diras2 4:intergenic:Gm10258-Mecom 4:intergenic:Med13l-n-R5s175 4:intergenic:Gm14977-Gm4994 4:intergenic:Dse-Tspyl1 4:intergenic:Gm13321-Pter 4:intergenic:Gm5912-Gins3 4:intergenic:Gm15387-Bai1 4:intron:Bach2 4:intergenic:AK129341-1700128F08Rik 4:intergenic:Tmpo-Rmst 4:intergenic:Gm23434-Cntnap5b 3:intergenic:Gm11235-Aldoart1 4:intergenic:Gm22509-Gm7069 4:intergenic:Gm2464-Ralyl 4:intergenic:Gm23624-4930593C16Rik 4:intergenic:Gm26489-1700028P15Rik 4:intergenic:Snx4-1700007L15Rik 4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Wdr11-Gm23847 4:intergenic:Gm22087-5S_rRNA 4:intergenic:Gm6429-Gm23029 4:intergenic:Tbx15-Gm12449 3:intergenic:Gm17091-Gm6455 4:intergenic:Crebbp-Gm5766 4:intergenic:C130060K24Rik-Tnip3 4:intergenic:Gm25658-Atp2b1 4:intergenic:Gm25875-Gm23835 4:intergenic:Gm11221-Gm11222 4:intergenic:Gm23900-Zfp804a 4:intergenic:Gm22025-Mid2 4:intergenic:Sorcs3-AC116451.1 4:intergenic:Sphkap-Gm25754 4:intron:Aplf 4:intron:Tsen2 4:intergenic:Nudt2-AI464131 4:intergenic:Gm6226-Ube3a 4:exon:Acsl3 4:intergenic:Gm25138-Gm8167 4:intergenic:Gm7931-Slit2 4:intron:4930544M13Rik 4:intergenic:Gm12614-Gm12615 4:intergenic:Gm12625-Gm12619 4:intergenic:Gm12627-Gm12613 4:intergenic:Gm12619-Gm12624 4:intergenic:Gm12621-Gm12627 4:intergenic:Gm12613-Gm12614 4:intron:6430548M08Rik 2:intergenic:Gm19965-Gm24030 4:intergenic:Gm23267-Med30 3:intergenic:Rbfox1-Gm23476 3:intron:Gpr98 3:intergenic:Vmn2r7/Gm26939-Gm25222 3:intergenic:Gm24295-Mef2c 3:intergenic:Vmn2r7/Gm26939-Gm25222 4:intron:Lphn3 2:intergenic:Bloc1s6-Sqrdl 4:intron:Pknox2 2:intergenic:Gm15177-Gm22522 4:intron:Fggy 3:intergenic:Gm13751-Gm13754 4:intergenic:Gm17399-Gm22198 3:intergenic:Gm17091-Gm6455 3:intron:Gm10217 3:intergenic:Foxb2-Prune2 3:intergenic:Atp1b3-BC043934 4:intron:Ttc7b 4:intergenic:Olfr885-Olfr887 4:intergenic:Gm24973-1700003E24Rik 3:intergenic:Gm23520-Gm12131 2:intron:RP23-131O4.2 3:intergenic:Gm20641-Nrg3 4:intergenic:Wnk2-Ninj1 3:intergenic:Gm9959-Gm9386 4:intergenic:Olfr890-Olfr891 4:intergenic:Olfr854-Olfr855 3:intron:Gpm6a 4:intron:4930525M21RIK/4930525M21Rik 4:intron:RP23-322E15.2/4930430D24Rik 4:intergenic:Cts7-Ctsm 4:intergenic:Galntl6-Gm26905 4:intron:Rpap3 3:intergenic:Cnga2-Magea4 2:exon:Mmrn2 4:intergenic:AL844604.1-Mpdz 4:intergenic:Col22a1-Gm23217 4:intergenic:Gm24387-Foxp1 4:intergenic:Mtnr1a-F11 4:intergenic:Gnat3-Gnai1 4:intergenic:Gm11042-Kcnh5 4:intergenic:Gm25818-Creb5 4:intergenic:Dennd5b-Gm10203 4:intron:Pde4b 4:intergenic:Gm26861-Gm23415 4:intron:Tmem132e 4:intergenic:Tmx1-Gm24474 4:intergenic:Gm25727-F830104G03Rik Off-target primers show all... show less...
272 / fw	`TTTGTACTTTAGCACAATAA GGG` `.................... ...` Cloning / PCR primers	63	84	39	55	60	74	0 - 1 - 9 - 30 - 167 0 - 0 - 2 - 8 - 0 207 off-targets	4:intron:Pkd1l1 4:intergenic:Arsj/Gm17225-Camk2d 3:intergenic:Gm25915-Gm4995 4:intergenic:Gm24432-Gm23411 2:intergenic:Dcc-Gm25509 4:intergenic:Gm25824-Gm26977 4:intergenic:Gm15427-St8sia4 4:intergenic:Pcdh20-Gm25886 3:intergenic:Abca16-E130201H02Rik 3:intergenic:Gm26354-Gm7536 4:intergenic:Mtfr2-Pde7b 4:intergenic:Gm26126-Gm11114 4:intergenic:Gm25550-Cdh9 4:intron:Fbxo8 3:intergenic:Gm11890-Gm11892 4:intergenic:Csmd1-Gm25665 3:intergenic:Gm4822-Tpm3-rs7 4:intergenic:Gm22618-Gm22976 4:intergenic:Rad51ap2-Gm23781 4:intergenic:Crh-4632415L05Rik 4:intron:Met 4:intron:March1 4:intergenic:Gm25670-Gm26255 4:intergenic:Gm22438-Gm22159 4:intergenic:Gm23061-Ostf1 4:intron:Chrdl1 4:intron:Csmd3 4:intron:Aox3 4:intergenic:Mical3-Pex26 4:intergenic:Gm15219-Cdkn3 4:intergenic:Rhob-Slc7a15 3:intergenic:Gm24471-Cartpt 4:exon:Sgms1 4:intergenic:Gm10780-Gm6634 4:intergenic:Sec62-Gpr160 4:exon:Tspan8 4:intergenic:Cacna1d-Dcp1a 4:intron:Cacna1e 2:intergenic:Gdap1-Gm25166 4:intergenic:Irf8-Gm26878 4:intergenic:Cnksr2-Gm8659 4:intergenic:Gm13899-St7 4:intergenic:Rpl27a-ps2-Slitrk1 4:intergenic:Mgat5b-Gm11728 3:intron:Col4a5 4:intergenic:Gm13326-Gm13325 4:intergenic:Gm23671-Dmd 3:intron:Tex11 4:intergenic:Gm25347-S1pr1 4:intron:Nkain3 4:intron:Pknox2 3:intergenic:Fam219a/Gm12404-Fam219a 4:intergenic:Dgkh-Vwa8 3:intergenic:Zbtb18-1700016C15Rik 4:intergenic:Gm12006-1700046C09Rik 4:intergenic:Gm14679-4931400O07Rik 4:intergenic:AC167724.1-Lrrn1 4:intergenic:Gm8604-MALAT1 3:intergenic:Gm15031-Gm25915 4:intergenic:Emc2-Tmem74 3:intergenic:Gm13615-Gm13518 2:intron:Gm12159 4:intergenic:2310002L09Rik-Frmd3 3:intergenic:5033411D12Rik-Gm24873 4:intron:Gm14697 2:intergenic:Gm8349-Arhgef26 2:intron:Gm2824 4:intergenic:Gm20581-Gm20579 3:intron:Snx29 4:intergenic:Gm25537-Gm24122 4:intron:Atp8a2 4:intron:Neb 4:intron:Spock3 4:intergenic:Gm7847-Gm23674 4:intron:Abhd12 4:intergenic:Asb9-Gm15216 4:intergenic:Casp12-Pdgfd 4:intergenic:Gm22828-Gm23960 3:intergenic:Gm26844-Gm4812 4:intron:Hsf3 4:intron:BC005624 4:intergenic:Gm2464-Ralyl 4:intergenic:8030451A03Rik-Gm11217 4:intergenic:Cat-Gm13996 4:intergenic:Gm22507-Trhde 3:intergenic:Ptgs1-Olfr334-ps1 3:intergenic:Nubpl-Arhgap5 4:intergenic:Snx7-Gm26871 4:intergenic:Gm23562-4930548G14Rik 4:intergenic:Gm24692-Sptssa 2:intergenic:Gm23218-Fyb 4:exon:Enah 4:intergenic:Gm14889-Gm14888 3:intergenic:Gm25144-Gm23147 4:intergenic:Gm15083-Atp2b2 4:intergenic:Smcp-Lce6a 4:intergenic:Bhlhe41-Sspn 2:intron:Uty 4:intergenic:Hnrnpa1l2-ps-Pcsk1 4:intron:Trpc7 4:intergenic:Gm14550-Gm14570 4:intergenic:Rbfox1-Gm23476 4:intergenic:RP23-448C3.1/Trpm3-Trpm3 3:intergenic:Gm23203-Gm23447 4:intron:Zfp644 4:intergenic:Gm25658-Atp2b1 4:intergenic:Gm22343-Ncoa1 3:intergenic:Gm24503-Ccrn4l 4:intron:Agbl1 4:intron:Gabrb1 4:intron:Iqsec1 4:intron:Dlgap2 3:intron:Rasgrf2 4:intergenic:Gm11846-n-R5s182 3:intron:Tc2n 4:intron:Asxl3 4:intergenic:n-R5s50-Gm23242 4:intergenic:Gm5611-Chordc1 3:intergenic:Cypt15-Gm14550 4:intergenic:Gm4862-Stpg2 2:intergenic:Runx1-AC124326.1 4:intron:Trdn 4:intergenic:P2ry1-B430305J03Rik/Rap2b 4:intron:Lrch2 4:intergenic:Gm22183-mmu-mir-378d 4:intergenic:Fbxo8-Hand2 4:intergenic:Gm22230-Gm23558 4:intron:Ubr1 4:intergenic:Gm23558-Galc 4:intergenic:Gm22283-Gm9008 4:intron:Trpv1 4:intron:Tmem45a 3:intergenic:Mbnl3-Gm7834 4:intergenic:Ngf-2410057H14Rik 4:intergenic:D3Ertd751e-Gm25714 2:intron:Gm2824 4:intergenic:Gm23011-Gm26691 4:intron:Ctnna3 4:intergenic:Gm5742-Gm22223 4:exon:Helb 4:intergenic:Grm8-6530409C15Rik 3:intergenic:Rmi1-Slc28a3 4:intergenic:Pcdh20-Gm25886 4:intergenic:Gm23987-Fam135b 3:intron:St18 4:intergenic:Gm10294-Kansl2-ps 4:intron:Cdh12 1:intergenic:Gm5594-Vmn2r-ps59 4:intron:Fstl5 4:intron:Prss57 4:intergenic:Gm3765-Rbmxl2 3:intron:Folh1 4:intergenic:Gm15554-Samsn1 4:intron:Osbpl9 4:intergenic:Gm12691-Gm12692 4:intron:Senp7 4:intergenic:Gm14597-Cxx1c 4:intergenic:Gm5776-Luzp2 4:intron:Lactb2 4:intergenic:Gm24999-Gm25000 4:intergenic:Ebf1-Gm12158 4:intron:Epas1 3:intergenic:Gm8429-Rpl21-ps4 4:intergenic:Twist1-Gm18025 4:intron:Ppat 4:intergenic:Gm10645/Tbc1d9-Tbc1d9 4:intergenic:Gm6984-Htr2a 4:intron:Dpf3 4:intergenic:4930583I09Rik-Gm25344 4:intron:Gm15631 4:intergenic:Gm25658-Atp2b1 4:intron:Adra1b 4:intron:Ppp1r1c 4:intergenic:Uaca-Gm10655 4:intron:Rbbp8 4:intergenic:Gm4842-Tcf4 4:intron:Cd74 4:intergenic:4921524J17Rik/1700051O22Rik-Gpt2 4:intergenic:Gm23520-Gm12131 4:intergenic:Gm26256-n-R5s61 4:intergenic:Atrx-Gm23656 4:intron:Inadl 4:intergenic:Gm22331-Gm24937 4:intergenic:Tenm2-Gm12128 4:intron:Cfh 4:intron:Ntrk3 4:intergenic:Satb1-RP24-465O15.1 4:intron:Lrp1b 4:intergenic:Gm17491-Slc20a2 4:intron:Ptprd 4:intron:Spink11 4:intergenic:Gm9726/mmu-mir-8099-2-Gm26055 3:intergenic:Gm13925-Gm20638 3:intron:Arhgap24 4:intergenic:Gm12084-Pnpt1 4:intergenic:Gm26127-Golga1 4:intergenic:Gm17384-3110047P20Rik/Gm9954 4:intergenic:Mvb12a-Tmem221 4:intergenic:Gm12289-Cox10 4:intron:Psd3 4:intron:Phka2 4:intergenic:Abhd17b-Tmem2 4:intron:Arap2 4:exon:Cntn5 4:intergenic:Cenpw-Gm22623 4:intergenic:Pde4b-Gm24869 4:intergenic:Gm8730-Cdh5 Off-target primers show all... show less...
21 / rev	`AGGGGCTGTGGATAAACTCA AGG` `.................... ...` Enzymes: SmoI Cloning / PCR primers	62	80	60	52	71	78	0 - 0 - 6 - 29 - 198 0 - 0 - 1 - 2 - 3 233 off-targets	3:intron:Snx27 2:intergenic:Gpr158-Gm13337 4:intergenic:Rnf157-Ubald2 3:intron:Mecr 3:intron:Cntln 3:intergenic:Sybu-Sybu/2310069G16Rik 4:intergenic:Gm23768-Gm22143 2:intergenic:Nctc1-H19 3:intron:Fnbp1 4:intergenic:Olfr483-Gm25424 4:intergenic:Ktn1-Gm6055 4:intron:Mrps27 4:intergenic:Gm24326-Gm25923 4:intergenic:Gm22183-mmu-mir-378d 4:intron:Foxp1 4:intron:Elmod3 4:intergenic:Gm24681-Gm23591 4:intergenic:Gm12377-Gm12376 4:intergenic:Gm2574-Gm24728 4:intergenic:Fer1l6-Gm20712 4:intron:Egflam 3:intergenic:Cacna1c-Gm25905 4:intergenic:Cd44-Pdhx 2:intergenic:Trpm7-Sppl2a 4:intron:Sh2d4b 4:exon:Syt7 4:intron:Rab14 4:intergenic:D3Ertd751e-Gm25714 4:intergenic:Gm10264-Adck1 4:intron:Fat3 3:intron:Stat6 4:intergenic:1700011I03Rik-Slc12a2 4:intron:Serpine3 4:intergenic:Gm22302-Uri1 3:intron:Tjp2 4:intron:Nhej1 4:intron:Grid1 3:intergenic:Mex3c-Gm26438 4:intergenic:Gm13731-Olfr1120 4:intron:Palm 3:intergenic:Vps13c-Gm22382 4:intergenic:Gm25485-Gm12460 4:intron:Nsd1 4:intergenic:Rgs17-Gm10945 4:intron:Wdr55 4:intergenic:Rps18-ps1-Gm11231 4:intron:Ptp4a2 4:intergenic:Gpr139-Gm23321 4:intron:Arhgef28 4:intergenic:Adarb1-Fam207a/2810425M01Rik 4:exon:Mir1957 4:intergenic:Aox4-Gm15759/Aox4 3:intergenic:Fpr2-Gm7535 4:intron:Phax 4:intron:Musk 4:intron:Dppa4 4:intergenic:Scgb1b3-Scgb2b7 4:intron:Stk32b 4:intergenic:Gm7784-B3gat2 4:intergenic:Gm12509-Gm12508 4:intergenic:Gm24343-Gpr126 4:exon:Gm15124/Gm15123 4:intergenic:Psen2-Gm5069 4:intron:9030622O22Rik 4:intergenic:Scgb2b26-Scgb1b26-ps 3:intron:Kcnd2 4:intergenic:Gm12723-Gm12726 4:intergenic:Rpl36-ps4-Msh6 4:intergenic:Gm23217-Gm24390 4:intergenic:Gm12448-Gm22505 4:exon:Snx22/Ppib 3:intron:Ttc8 4:intergenic:Dock9-Ubac2 4:intergenic:Snx4-1700007L15Rik 4:intron:Rab13 4:intron:AB124611 4:exon:Pcsk1n/Eras 4:intron:Tcf7l2 4:intergenic:Glrx5-Tcl1b2 4:intergenic:Glrx5-Tcl1b2 4:intergenic:Ntng2-6530402F18Rik/Ntng2 4:exon:Angptl4 4:exon:Fam65c 4:intergenic:Gm23209-Rere 4:intron:Rad23b 4:intergenic:Gm7168-Dact2 4:intergenic:Lamp2-Gm7598 3:intergenic:Tdrd3-Rps3a2 4:intergenic:Gnai2-Slc38a3 4:intergenic:Gm26288-Atp10a 4:intergenic:Tenm1-Gm24763 4:intron:Slc7a8 4:intron:Rhox2d 4:intergenic:Slc36a3-Slc36a2 4:intron:Acss1 4:exon:Cmklr1 4:intergenic:Cmip-Plcg2 4:exon:Ankrd42 4:exon:A930011O12Rik/Mir124a-1/Mir3078 4:intron:Amph 4:intron:1700112E06Rik 4:intron:Nebl 3:intergenic:Fabp5-Gm9833 4:intron:Sbno2 4:intron:Abca16 4:intergenic:Lmbr1l-Tuba1b 4:intron:Celsr1 3:intergenic:Tubb4b-ps1-4921511H03Rik 4:intron:Ddx58 4:intergenic:Gm12724-Gm12731 4:intergenic:Tshz2/A630075F10Rik-Zfp217 4:intergenic:Gm12539-Palm2/Gm20459 4:intron:B230118H07Rik 4:intron:Gpr98 4:intergenic:Gm7536-Gm24704 4:intron:Sh3bp2 4:intron:Tmem235 4:intergenic:Gm13847-Gm13846 3:intergenic:Dock8-Kank1 2:intergenic:Klhl2-Gm26210 4:intergenic:Fam69a-Gm9850 3:intron:Ppp2r5e 4:intergenic:Lrig1-Gm7833 4:intron:Phf1 3:intron:Sgk1 4:intergenic:Gm10275-Cenpw 4:intergenic:Hhipl1-Hhipl1/4930478K11Rik 3:intergenic:Ipo13-Artn 4:intron:Tbl3 4:intron:Iqca 4:intergenic:Gm11248-Gm11185 4:intergenic:Gm10304-Dab1 4:intron:Uhrf2 4:intron:Plec 4:intergenic:Gm26321-Gm13597 4:intron:Rptor 4:intergenic:Mir3086-Eno4 4:intron:Sgcd 4:intergenic:St7-Wnt2 4:intergenic:Rps18-ps2-Gm23423 4:intergenic:Gm22597-Gm24514 3:intron:Prdm12 4:intergenic:4930431F12Rik-Gm10205 4:intergenic:Hpca-Gm15906 4:intron:Bai1 4:intron:Stpg2 4:intergenic:Porcn-Ftsj1 4:intergenic:Shisa9-Gm6327 4:intergenic:Gm23625-Mad2l1 4:exon:Poc5 3:intergenic:Sp4-Gm25577 4:intergenic:Gm6600-Gm8837 3:intron:Fam45a 4:intergenic:Rbbp6-Tnrc6a 4:intergenic:Gm6985-Pcyt1b 3:intron:Zbtb7c 4:exon:Gabpb2 4:intron:Nfasc 2:intergenic:Thsd4-Lrrc49 4:intergenic:Kcnb2-Gm25227 4:intergenic:Gm14006-Gm14008 4:intron:Fnip1 4:intergenic:Gm25476-Gramd3 4:intergenic:Lrpap1-Adra2c 4:intron:Cdh13 4:intergenic:Gm13400-Ptges 4:intron:9030204H09Rik 4:intron:Prim2 3:intergenic:Spata19-Opcml 4:intergenic:Mafb-Gm14221 3:intergenic:Fut2-Sec1 4:intergenic:Gm14065-Gm14067 4:intergenic:Ptprn2-Rapgef5 4:intergenic:Gm24458-Ifi44 4:intron:Morf4l1 4:intron:Vip 4:intergenic:Sh2d6-Capg 4:intron:Thada 4:intergenic:Gm4409/Lrrtm4-Lrrtm4 3:exon:Pygl 4:intergenic:Klk1b8-Klk1b1 4:intergenic:Chst10-Nms 4:intergenic:Gm26976-Cand1 4:intergenic:3100003L05Rik-Gm21957 4:intron:Hnf1b 4:exon:D8Ertd82e 4:intergenic:Nsmce2-Trib1 3:intergenic:Gm23822-4921504A21Rik 4:intergenic:A930011G23Rik-Gm16227 4:intergenic:Amer2-Spata13 4:intergenic:Gng4-B3galnt2 4:intergenic:Gm10557-Map3k8 4:intergenic:Chst1-Gm13816 4:intergenic:Gm26619/Gm10260-Arhgef28 4:intron:Csmd2 4:intergenic:Tfap2c-Gm22773 2:intergenic:Gm5593-Vmn2r60 3:intergenic:Gm6214-Pdha2 4:intergenic:Gm15394-Gm24547 4:intergenic:BC030500-Gm24847 4:intergenic:Olfr1318-Lpcat4 4:intergenic:Gm13452-Gm13453 4:exon:Naa60/1700037C18Rik 4:intergenic:Cacna1h-Tekt4 4:exon:Col9a3 4:intergenic:Gm22536-Gm6632 4:intergenic:Gm6884-Taar7f 4:intergenic:Gm12181-Gm24422 4:exon:Oxtr 4:intergenic:Uaca-Gm10655 4:intergenic:Gm26256-n-R5s61 4:intergenic:Gm24946-Plch1 4:intron:Cdkal1 4:intron:Dscam 4:intergenic:Gm25659-Gm25644 4:intron:Lpin3 4:intergenic:mmu-mir-6336-Lrp1b 4:intergenic:Gm25401-Mir100 4:intergenic:Vmn1r46-Vmn1r-ps29 4:intron:Nxph1 4:intergenic:Gm20696/Foxp1-Gm20705 4:intron:Ambp 4:intergenic:Higd1a-Ackr2 4:intergenic:Srrm4-4930569F06Rik 4:intergenic:AC154801.1-BC040587 4:intergenic:Errfi1-Park7 4:intergenic:4930554G24Rik-Gm12006 4:intergenic:n-R5s21-Cpn1 4:intron:Ankrd36 4:exon:Carm1 4:intergenic:Gm22444-mmu-mir-6238 4:intron:Madd 4:intron:Fbxo27 Off-target primers show all... show less...
592 / rev	`ACATACTTGACCACAGAATA TGG` `.................... ...` Cloning / PCR primers	62	87	41	30	71	75	0 - 0 - 3 - 21 - 188 0 - 0 - 2 - 1 - 5 212 off-targets	4:intergenic:Gm26475-Gm24522 2:intergenic:Gm25096-Gm25634 2:intron:Cnksr3 4:intron:Chsy3 4:intergenic:Gm12183-Gnb2l1/Gm25296/Snord96a/Snord95 4:intergenic:Gm8993-Gm9001 4:intron:Stat5b 4:intron:Fam126a 4:intergenic:Grxcr1-Gm23605 4:intron:Hnmt 4:intergenic:Narg2-Gm4978 4:intergenic:Gm8991-Gm6673 4:intergenic:Dscam-Bace2 4:intron:Pde9a 4:intergenic:Gm5513-Gm24319 4:intergenic:Gm24160-Ap1s3 4:intergenic:Gm26206-Xkr4 4:intergenic:Arfgef1-Cpa6 4:intron:Ralgapa1 4:intergenic:Pdgfd-Gm23097 4:intron:Lmo4 4:intron:Pex7 4:intron:Il1f6 4:intergenic:Gm22698-Tmem174 4:intergenic:Defa21-Gm23142 4:intergenic:Defa22-Gm26240 3:intergenic:Kitl-Gm22918 4:intron:Mum1l1 4:intergenic:Gm23892-Gm25382 4:intergenic:Vmn2r79-Nox4 4:intergenic:Gm25813-Gm23666 4:intergenic:Id2-Gm22527 4:intergenic:Gm25905-Dcp1b 4:intergenic:Pds5b-Kl 4:intron:Pde4b 4:intergenic:Ednrb-RP24-312G4.2 4:intergenic:Fam184a-Man1a 4:intergenic:Gm25549-Mapkapk2 3:intergenic:Gm10192-Gm12696 4:intron:Astn2 4:intergenic:Gm23831-1700017G19Rik 4:intergenic:Ednrb-RP24-312G4.2 4:intergenic:Gm14249-1700101G07Rik/Gm11011 4:intergenic:Gm13974-Gm13973 4:intergenic:Tfcp2-Pou6f1/POU6F1 4:intergenic:Gm11499-Gm11500 3:intron:Ppp1r16b 4:intergenic:Gm11221-Gm11222 4:intergenic:Gm13602-Gm13598 4:intron:Gpc3 4:intron:Gm8091 4:intergenic:4930533B01Rik-Fmn1 4:intron:Aig1 3:intergenic:Gm4899-Gm5354 4:intron:Cmbl 4:intron:Fam129b 4:intron:Astn2 4:intergenic:Gtf2h1-Ldha 4:intergenic:Gm9578-Gm22891 4:intron:Ctage5 3:intergenic:Olfr131-Olfr132 4:intergenic:Vmn2r6-Vmn2r-ps9 4:intergenic:Vmn2r4-Vmn2r-ps6 4:intron:Ttc27 4:intergenic:Zfp563-Morc2b 4:intergenic:Prr16-Gm25785 4:exon:Eny2 4:intergenic:Gm11349-Gm11350 4:intron:3100003L05Rik 4:intergenic:Gm11506-Utp18 4:intergenic:Gm23642-Lrfn2 4:intergenic:4930550L24Rik-Fgf13 4:intron:Sh3glb1 4:intergenic:Gm12010-Gm12011 4:intron:Sycp1 4:intergenic:Gm22191-Gm22726 4:intron:Grid2 4:intergenic:Sema3f-Rbm5 4:intergenic:4931408C20Rik-n-R5s209 3:exon:Tmcc1 4:intergenic:Gm25828-Nhsl2 4:intron:Arhgef2 4:intergenic:Gm6317-Gm25383 4:intergenic:Gm15943/Fbxo32-Klhl38 4:intergenic:Gm9835-Ppp1r9a 4:intergenic:Gm21838-Gm21803 4:intergenic:Gm21783-Gm21779 4:intergenic:Gm20901-Gm21865 4:intergenic:Gm21767-Gm20822 4:intergenic:Gm21805-Gm21241 4:intergenic:Gm21763-AC201846.1 4:intergenic:Gm21779-Gm21247 4:intergenic:Gm21800-Gm21729 4:exon:Ccar2 4:intergenic:Tnfsf13b-Myo16 4:intergenic:Gm12412-Aldh1b1 3:intergenic:Bloc1s6-Sqrdl 4:intergenic:Gm15074-Gm4996 4:intergenic:Gm26076-Gm25519 4:intron:Dlgap1 4:intergenic:Olfr1490-Olfr1491 4:intergenic:Gm22533-Dnajc6 4:intergenic:Gm11873-Nkain3 3:intergenic:Ptger3-Cth 4:intergenic:Gm8216-Mir3472 4:intergenic:B430319F04Rik/Ampd3-Ampd3 4:exon:Surf6 4:intergenic:Gm11765-Gm11411 4:intron:Gm5142 4:intron:Vmn2r76 4:intergenic:Olfr857-Olfr58 4:intergenic:Mmp7-Gm10709 4:intron:Plce1 4:intergenic:St3gal6-Gm813 4:intergenic:Vmn1r180-Vmn1r181 4:exon:Strbp 4:intergenic:Prkd1-Gm22088 4:intron:Eml1 4:intergenic:Pcdh17-Gm23926 4:intergenic:Rassf10-Gm23662 4:intergenic:Gm26468-Rpsa-ps10 4:intergenic:Gm25905-Dcp1b 4:intergenic:Gm24696-Myc 4:intron:Lama1 4:intron:Tmcc3 4:intergenic:Adck1-Nrxn3 4:intron:Tbl1xr1 2:intron:Gna14 4:intron:Gpr125 4:intergenic:Gm13052-Gm13018 4:intergenic:Gm7069-Gm10649 4:intergenic:Arhgap19-Frat1 4:intergenic:Rfwd2-Gm10530 4:intergenic:Gm25598-BC002059 4:intergenic:Gm26976-Cand1 4:intergenic:Trim2-Fhdc1 4:intron:Lrrc49 4:intergenic:Olfr859-Olfr860 4:intron:Rapgef1 4:intergenic:Gm26190-B3gat1 3:intron:Mapkbp1 4:intron:4833422M21Rik 4:intron:Nsmce2 4:intron:Reln 4:exon:Olfr348 4:intron:Rgl1 4:intergenic:Gm12477-Gm12478 4:intergenic:Qpct-Cdc42ep3 4:intron:Fndc3c1 4:intergenic:Trpc3-Gm12377 4:intergenic:9330185C12Rik-Cntnap5a 3:intron:Dzank1 4:intergenic:Gm23183-Apbb2 4:intron:Pnpla7 4:intergenic:Iws1-Proc 4:intergenic:Lrig2-Gm26091 4:intergenic:Mir470-Mir465c-1 4:intergenic:Mir465c-1-Mir465b-1 4:intergenic:Mir465b-2-Mir465 4:intergenic:Mir465b-1-Mir465c-2 4:intergenic:Mir465c-2-Mir465b-2 4:intron:Sh3gl3 3:intron:Lrrc4c 4:intron:Dusp27 3:intergenic:Gm2165-Gm7408 3:intergenic:Gm3657-Gm3669 3:intergenic:Gm3681-Gm14632 3:intergenic:Gm7365-Gm7375 3:intergenic:Gm4836-Gm7398 3:intergenic:Gm2318-Gm14553 3:intergenic:Gm2288-Gm14552 3:intergenic:Gm10487-Gm21447 3:intergenic:Gm14974-Gm7438 3:intergenic:Gm2101-Gm7386 4:intergenic:Grm8-6530409C15Rik 4:intergenic:Gm24326-Gm25923 4:intergenic:Gm13211-Gm13254 4:intergenic:Fam110c-6030469F06Rik 4:intergenic:C1ql3-Rsu1 4:intron:Gm12836 4:intergenic:Epha5-Gm24626 4:intron:Wdr1 4:intron:Gm6999 4:intergenic:Neto1-Cbln2 4:intergenic:Grik2-Ascc3 4:intergenic:Gm25480-Gm11220 4:intergenic:Gm23986-Gm24929 4:intergenic:Gm9621-Gm24200 4:intergenic:Gm6973-4932429P05Rik 4:intergenic:A630031M04Rik-1700112K13Rik 4:intergenic:Gm12413-Sh3gl2 4:intergenic:9130204L05Rik-Gm23697 4:intergenic:Mmp16-Gm11852 4:intergenic:Gm14624-Gm22103 4:intergenic:Gm8730-Cdh5 4:intron:Rspry1 4:intron:Crisp1 4:intergenic:Mrs2-Dcdc2a 4:intergenic:Ccser1-Gm22212 4:intron:Slc6a5 4:intergenic:M5C1000I18Rik/C2cd4b-C2cd4a 4:intergenic:Gm25113-Car10 4:intergenic:AC158354.1-Gm4804 4:intergenic:Cyb5r4-Mrap2 4:intergenic:Rasa1-Cox7c 4:intergenic:Gm25132-Dclk1 4:intergenic:Gm13599-Gm21830 4:intergenic:Gm6686-Chd1 4:intergenic:Pten-Gm22482 4:intergenic:Gm5839-Gm25440 4:exon:Nat1 4:intron:9430007A20Rik Off-target primers show all... show less...
620 / rev	`ATGGAAAGACTCCATTGTTG AGG` `.................... ...` Enzymes: Bsp1286I, BaeGI Cloning / PCR primers	62	79	56	41	71	46	0 - 1 - 11 - 39 - 186 0 - 0 - 0 - 2 - 6 237 off-targets	3:intron:4930544I03Rik 4:intergenic:Gm16564-Kif26b 4:intergenic:F630028O10Rik/Mir223-Vsig4 3:intergenic:Gm9726/mmu-mir-8099-2-Gm26055 4:intergenic:Tmem200c-Epb4.1l3 4:intergenic:Gm24774-Pcdh17 4:intergenic:Rnf217-Gm7909 4:intron:Pde4b 4:intron:Pid1 4:intergenic:Ephb1-n-R5s88 4:intron:Phf17 4:intron:Col22a1 4:intergenic:Gm15180-Gm15182 4:intron:3110043O21Rik 4:intergenic:Sh3tc2-Adrb2 4:intergenic:Gm15060-U6 4:intergenic:Rnpc3-Col11a1 4:intron:Galntl6 4:intron:Zfp668 4:intergenic:Ptger4-AC133283.1 3:intergenic:Gm23520-Gm12131 4:intron:Runx1 4:intergenic:Gm24066-Gm6395 4:intergenic:Nmi-Tnfaip6 3:intron:Lrrc7 4:intergenic:Gm14971-Gm14969 4:intergenic:4930552N02Rik-Olfr273 4:intergenic:Gm24633-Gm25568 3:intron:Lrp12 4:intergenic:Gm22870-Gm7936 4:intron:Vav3 4:intron:Ccdc129 4:intron:Vps13d 4:exon:Slc38a8 3:intergenic:Gm25266-Cradd 3:intergenic:Gm6586-Gm10482 4:intron:Hhatl 3:intergenic:Clec14a-Gm17529 4:intergenic:Sh3bp4-Agap1 4:intron:Htr4 3:intergenic:Gm15399-Gm9372 4:intergenic:5033403F01Rik-Gm23972 3:intron:Gm16630 4:intergenic:5830418K08Rik-Smco4 4:intron:Ltbp1 2:intergenic:Xpo7-Dok2 4:intron:Slc4a7 4:intergenic:Gm11879-Gm11883 4:intergenic:Gm26018-BC018507 3:exon:Zfp572 4:intron:Lrfn5 2:intergenic:Gm22507-Trhde 4:intron:Nox4 4:intron:Slco4c1 4:intron:Vav3 4:intergenic:4930527A07Rik-Pax6os1/Gm13890 4:intron:Cd226 3:intron:Stag1 3:intergenic:Grxcr2-Sh3rf2 4:intergenic:Sntb1-Has2 4:intergenic:S100a6-S100a7a 4:intergenic:Gm9956-Gm24285 4:intergenic:Gm24127-Pcdh9 4:intron:Slc35f1 3:intron:Lrp2 4:intergenic:Gm5593-Vmn2r60 2:intergenic:Gm24246-Pik3c3 4:intergenic:Gm12458-Gm25485 4:intergenic:Cdr1-Gm14665 4:intergenic:Cadm1-Nxpe2 4:intergenic:Cntnap5a-Gm19965 4:intergenic:Pdzd2-Gm24302 4:intergenic:Pou6f2-Vps41 3:intergenic:Grem2-Rgs7 4:intron:Tmem178b 4:intron:RP23-302F9.3 4:intergenic:Gm6489-Gm10307 4:intergenic:Rpl21-ps4-Gm3839 4:intergenic:Gm8832-Frmpd4 4:intron:Gm16630 4:intron:Maml3 4:intergenic:Gm23447-Gm23773 4:intron:Ubap2l 4:intergenic:Mir1932-Rnf213 4:intergenic:Gm23760-Gm23013 4:intergenic:Gm14991-Kir3dl2 4:intron:Glra2 4:intergenic:Gm5974-Gm23240 4:intergenic:Dnajb6-Il6 4:intergenic:Gm26619/Gm10260-Arhgef28 3:intron:Nxf3 4:intron:Dpyd 4:intergenic:Gm23892-Gm25382 3:intergenic:Eva1a-Tacr1 4:intron:Zfp12 4:intergenic:Gm7745-Lrfn5 4:intron:Ccdc141 4:intron:Tmprss7 4:intergenic:Gap-CAAA01098150.1 4:intergenic:Spaca1-Gm25623 2:intergenic:Gm25509-n-R5-8s1 1:intron:Cep290 4:intron:Kcnd3 4:intron:Foxp2 3:intergenic:Cyp4a30b-ps-Gm12833 4:intergenic:Gm11506-Utp18 4:intergenic:Gm26345-Gm10566 4:intergenic:Gm14666-Gm14821 4:intergenic:Gm11224-Gm11225 4:intron:Diap2 4:intergenic:Atg10-A830009L08Rik 4:intergenic:Gm24728-Lmo4 4:intergenic:Gm25765-Gm25806 4:intron:Ugt8a 3:intergenic:Gm14467-Gm17014 4:exon:Kidins220 4:intergenic:Ddx11-Soga2 4:intergenic:Gm25485-Gm12460 4:intergenic:Gm26326-Dnajb9 3:intergenic:Defb41/Gm15386-Gm24075 4:intergenic:Gm22122-Gm16630 4:intron:Ophn1 4:intergenic:Rpl38-ps2-Gm11077 4:exon:Tmem178b 4:intergenic:Gm20516-Gm21947 4:intergenic:mmu-mir-6379-Gm10356 4:intergenic:Gm23337-Gm26081 4:intergenic:Gm22011-Mthfr-ps1 4:intergenic:Slco6c1-D1Ertd622e 4:intergenic:Tsku-Gucy2d 4:intergenic:2310065F04Rik-Myh2 4:intergenic:Nav3-E2f7 4:intergenic:Gm14547-Cald1 3:intron:Ptprn2 4:intron:Vsnl1 2:intergenic:Tpbg-Ube2cbp 4:intergenic:5830418K08Rik-Smco4 4:exon:Smpdl3b 4:intron:Syt1 3:intergenic:Gm25130-Gm22970 4:intergenic:Olfr1251-Olfr1252 4:intergenic:Gm15496-Kcnmb2 4:intron:Ubtd2 4:intergenic:Idh2-Sema4b 3:intergenic:Smlr1-Tmem200a 4:intergenic:Vmn1r49-Vmn1r50 3:intron:Mettl24 4:intron:Esrrb 4:intron:Cspp1 4:intergenic:Srek1ip1-Fam159b 3:intergenic:Snrpn/Snurf-Snrpn 4:intergenic:Fam169b-Gm5334 3:intron:Gm13490 4:intron:Nav2 4:intergenic:Mtpn-Gm23273 3:intergenic:Ndfip1-Spry4 4:intergenic:Skint11-Trabd2b 4:intergenic:Tlr5-Disp1 4:intron:Galnt7 4:intergenic:Gm25578-Actr3 3:intergenic:Gm22482-Rnls 2:intergenic:Ctnna3-Gm22829 4:intergenic:Ccdc91-1700049E15Rik 4:intergenic:5430402E10Rik-Gm14750 3:intergenic:Gypc-Gm25826 4:intergenic:Gm25258-Hao1 4:intron:Grin2a 4:intergenic:Stpg2-mmu-let-7j 4:intron:Xkr5 4:intergenic:Gm23186-Arid1b 4:intergenic:Gm10097-Syne1 4:intergenic:Pcmtd2-Polr3k 4:intergenic:Cldn1-Cldn16 4:intergenic:Hdhd1a-Prr16 4:intergenic:Tapt1-Ldb2 3:intron:Cngb3 4:intergenic:Gm16077-Card6 4:exon:A430110L20Rik 4:intergenic:Gm6771-Ptchd4 4:intergenic:AC154801.1-BC040587 4:intergenic:F830001A07Rik-Rora 3:intergenic:Gm20682-3300002A11Rik 4:intron:Gm12531 4:intron:Cep110 4:intergenic:4930578C19Rik-Gm6923 2:intergenic:Fcgr4-Fcgr3 2:intergenic:C3ar1-Necap1 3:intron:Pkhd1 4:intergenic:Gm14293-Gm14294 4:intergenic:Maoa-Maob 4:intergenic:Ptprd-Gm11242 4:intron:Sox6 4:intergenic:Gm24728-Lmo4 4:intergenic:Gm26197-Akap11 4:exon:Cdh5 4:intron:Mvb12b 4:intron:4930555F03Rik 4:intergenic:Gm10029-Gm22689 3:intron:Gm13388 4:intergenic:Lrch1-5031414D18Rik 4:intron:Ano2 3:intron:Arhgap6 3:intergenic:Gm13794-Gm13796 4:intergenic:Gm8935-Gm2464 4:intergenic:Tmem126b-Dlg2 4:intron:Pvrl4 4:intergenic:Ccdc172-Pnlip 4:intergenic:Gm24369-n-R5s50 4:intron:Hnmt 4:intergenic:Slc38a4-Amigo2 4:intergenic:Gm24887-Rab11fip4 4:intergenic:Gm9889-A930005H10Rik 4:intergenic:Slc4a4-Gc 4:intergenic:Gm21232-Gm27044 4:intergenic:4430402I18Rik-Cdc37l1 3:intron:Aldh3a2 2:intergenic:Gm7224-Vmn2r59 4:intergenic:Gm23131-Lphn2 4:intron:Macrod2 4:intron:Slc1a3 4:intergenic:Gm25383-Gm24863 4:intergenic:Tec-Gm9870/Slain2 4:intergenic:Nrbf2-Egr2 4:intergenic:4930577H14Rik-Izumo3 3:intergenic:Gm25051-Gm1818 3:intergenic:Igf2bp2-Gm25810 2:intergenic:n-R5s42-Fam19a5 4:intron:Dnah8 4:intron:Med14 4:intergenic:Schip1-Gm22073 4:intergenic:Gm9864-Gm23439 4:intron:Sccpdh 4:intergenic:Fst-Gm25950 3:intron:A330074K22Rik 4:intergenic:Gm831-Gm12962 4:intron:Pde4dip 2:intron:Grin2b Off-target primers show all... show less...
471 / fw	`AGTCATTGTCATATCCTTCA TGG` `.................... ...` Inefficient Enzymes: Hpy166II, NlaIII, HpyAV Cloning / PCR primers	61	80	41	37	56	80	0 - 0 - 5 - 27 - 183 0 - 0 - 1 - 7 - 9 215 off-targets	3:intron:Camk2b 3:exon:Bcl11a 4:intergenic:Gm5593-Vmn2r60 4:intron:Gm5893 4:intron:Agbl1 4:intergenic:Gm25454-n-R5s165 4:intergenic:Gm22183-mmu-mir-378d 4:intergenic:Gm13578-Grb14 3:exon:Sh2d4a 3:intergenic:Ryr2-Mtr 4:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Gm11214-Tnfsf15 4:intergenic:Gm22438-Gm22159 4:intron:Park2 4:intergenic:Gm13988-Sema6d 4:intergenic:Olfr419-Mptx1 4:intron:Dtna 4:intergenic:Phyhipl-4930533K18Rik 4:intron:Tmeff2 4:intergenic:Gm13988-Sema6d 4:intergenic:Mptx2-Darc 3:intergenic:Gm26149-Homer2 4:intergenic:Gm23644-Gm15442 3:intergenic:Gm24756-Prss38 4:intergenic:Kcnma1-Gm6158 4:intergenic:Gm23036-Gm22034 4:intergenic:Gm22331-Gm24937 4:intergenic:Gm24957-Gm22492 4:intergenic:Tnfsf8-Rpl17-ps4 4:intron:Tbc1d22a 4:intron:Hspg2 2:intergenic:Slc35f1-Gm25664 4:intergenic:Slco6c1-D1Ertd622e 4:intron:Alpk3 4:intron:Pde4d 3:intergenic:Gm22610-Dpp10 3:intergenic:Gm25103-Dph6 4:exon:Lrrc19 4:intergenic:Nus1-Zfa-ps 4:intron:4933406K04Rik 4:intergenic:Usp31/1700069B07Rik-Scnn1g 2:intergenic:Gm24587-Dcn 4:intergenic:Gm25650-Gm23584 4:intron:Grm7 4:intergenic:Kif26a-A730018C14Rik 3:intron:Hecw1 4:intergenic:Enox1-Gm1587 4:intergenic:Gm26076-Gm25519 4:intron:Asun 4:intergenic:Bdnf-Gm13932 4:exon:Gm7206 4:intron:Zfp712 4:intergenic:Hhip-Gm5909 4:intergenic:1700122E12Rik-Farsa 4:exon:Rpl9-ps4 4:exon:Gm5451 4:exon:Rpl9 4:exon:Rpl9-ps7 4:exon:Rpl9-ps6 4:exon:Gm12110 4:intergenic:RP23-442I7.1-Gm17308 4:intron:Col13a1 4:intergenic:Cacna1d-Dcp1a 4:intron:Gm7568 4:intergenic:Gm13584-Lhx2 4:intergenic:Prkag2-1500035N22Rik 4:intron:Mlip 4:intergenic:Fbxl14-Wnt5b 4:intron:Tmcc3 4:intergenic:Tm2d3-Gm7551 4:intergenic:Ttc9-Map3k9 4:intergenic:Magi2-Gm25335 3:intergenic:Sh3tc2-Adrb2 4:intergenic:Gm24921-Fscb 3:intergenic:Gm6226-Ube3a 4:intergenic:Galntl6-Gm26905 4:intron:Scn8a 3:intron:Fam172a 3:intergenic:Gm25570-Gm15631 4:intergenic:Osbpl3-Gm26289 4:intron:Dpp10 4:intron:Dpp10 4:intron:Dpp10 4:intron:Cnksr2 4:exon:Cpeb3 4:intron:Cecr2 4:intron:Mapk10 4:intron:Slc35f1 4:intergenic:Macrod2-Rpl26-ps5 3:intron:Jak2 3:intergenic:Tmtc1-Gm6313 4:intergenic:Gm22777-Spock1 4:intergenic:Gm11804-8430436N08Rik 4:intergenic:Sema3d-Gm23243 4:intron:Fras1 4:intergenic:Gm25602-Tbc1d32 4:intron:Mtr 4:intergenic:Ly86-Rreb1 3:intron:Epas1 4:intergenic:Gm11826-Gm11825 4:intergenic:Shisa6-Gm12296 4:intron:Adcy1 4:intron:Itpr2 4:intergenic:Dnm3-Dnm3os/Mir199a-2/Mir214 4:intergenic:Pvrl3-Gm6912 4:intergenic:Fto-Gm3235 4:intergenic:Acaa1a-Xylb 4:intergenic:Gm23075-Gm22853 4:intergenic:Rfpl4b-Lama4 4:intergenic:Gm22087-5S_rRNA 3:intron:Slc10a7 3:intergenic:Cd22-Mag 4:intergenic:Gm26137-Gm23733 4:intergenic:Gm11468-Gm14268 4:intergenic:Dpp10-Gm10543/Dpp10 4:intron:Wipi1 4:intergenic:D730048I06Rik/D730048I06RIK-9230110F15Rik 4:exon:Dnah11 4:intergenic:Banp-Gm22 4:intron:Fras1 4:intergenic:Gm22829-Gm22594 4:intron:Epyc 3:intron:Magi2 4:intergenic:Gm4773-Gm14760 4:intergenic:Spp2-Glrp1 4:intergenic:Cdca7l-Gm20613/Cdca7l 4:intergenic:Lrrtm3-Ctnna3 4:intergenic:Edem1-Gm20387 3:intergenic:Gm13463-Lrp1b 4:intergenic:Gm12009-Gm24707 3:intergenic:Kcnma1-Gm6158 3:intron:Il7 4:intergenic:Gm24404-Nrg3 4:intergenic:4930421P07Rik-Zfp518b 4:intergenic:Gm24326-Gm25923 4:intron:Rbms1 4:exon:Cacng7 4:intergenic:Gm16043-Gm16039 4:exon:Slc36a4 4:intron:Pptc7 4:intergenic:Gm7224-Vmn2r59 4:intron:Fggy 4:intron:Ntrk3 4:intergenic:Gm9897-Fzd1 4:intergenic:1700039E15Rik-Trpm4 4:intron:Fam189a1 3:intergenic:Pbx1-mmu-mir-6348 4:intergenic:Gm6634-Gm8604 4:intergenic:Gm6900-Vmn1r-ps52 4:intergenic:A630014C17Rik-Vdac1 4:intron:6530403H02Rik 2:intron:A230006K03Rik 2:intergenic:A230006K03Rik-Ube2nl 4:intergenic:Zranb1-Gm15718 4:intergenic:Sgcd-Gm12178 4:intergenic:Shisa9-Gm6327 4:intergenic:Rhcg-Ticrr 4:intergenic:Neu2-Inpp5d 4:intron:Map3k11 4:exon:Slc5a1 4:intergenic:Gm22848-Rp1 4:intergenic:Olfr798-Olfr799 4:intron:Mta1 4:intron:A830080D01Rik 4:intergenic:Pex14-Gm9506 3:intergenic:Gm14094-U6 4:intergenic:Gm6767-Cblb 4:intergenic:Nrg3-4930529F24Rik 4:intergenic:Gm23661-Gm23067 4:exon:Prdm11 4:exon:Trpc6 4:intron:Reps1 4:intron:Ugt2b36 3:intergenic:Spock1-Klhl3 4:exon:Ubac1 3:intergenic:A230006K03Rik-Ube2nl 4:intron:Ptprq 4:intron:Prkce 4:intergenic:Gm22819-Cyb5 4:intergenic:Dsel-Gm22331 4:intergenic:Gm23038-4930570G19Rik 4:intergenic:Sema3d-Gm23243 4:intergenic:Kctd1-Aqp4 4:intergenic:Pigp/Ttc3-Ttc3 4:intron:Dennd5b 4:intergenic:Pcdh7-Gm22536 4:intergenic:Ankrd34c-Mir184 4:exon:Gm6317 4:intergenic:Glyat-Gm24521 4:exon:Ntan1 4:intergenic:Eya4-Rps12 4:intron:Arhgap15 2:intergenic:Gm23325-Pak3 4:intron:Ptpn3 4:intergenic:Gm25051-Gm1818 4:intergenic:Gm11797-Fam110b 3:intron:Stk32b 4:exon:Peg3 4:intergenic:Gm25169-Efnb2 4:intergenic:Gm24122-Gm23036 4:exon:AW209491 4:intergenic:Klhl32-Ndufaf4 4:intergenic:Gm9931-Gm22966 4:intron:Maoa 4:intergenic:Dscaml1-Gm22069 4:intron:Slco1a4 4:intron:Atp6v1a 4:intergenic:Adra2c-E130018O15Rik 4:intergenic:Gm16256-Slc48a1 4:intergenic:Olfr750-Rnase4/Ang 4:intergenic:Auts2-Gm25722 4:intron:Grid2 4:intergenic:Gm23186-Arid1b 4:intergenic:Fhl2-Gm8210 4:intergenic:Hint1-Gpx3 Off-target primers show all... show less...
770 / fw	`TAATTGTATGGAACTTCATT GGG` `.................... ...` Inefficient Enzymes: SmiMI, BtsCI Cloning / PCR primers	60	84	29	34	52	76	0 - 2 - 5 - 15 - 142 0 - 0 - 0 - 4 - 3 164 off-targets	2:intergenic:Gm26861-Gm25496 3:intergenic:Slc22a22-Zhx2 2:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Hs6st2-Gm14581 2:intron:Hmcn1 4:intergenic:Gm4795-Prep 4:intergenic:Gm26256-n-R5s61 4:intergenic:Kcne4-Gm5830 4:intergenic:Gm25684-Gm9966 4:exon:Gm20682 4:intergenic:Gm9966-Tenm4 4:intergenic:Gm24552-Gm22080 4:intergenic:Ces1e-Ces1f 4:intergenic:Frmpd4-Gm8832 4:intergenic:Rpl10l-Mdga2/MDGA2 4:intergenic:Cmklr1-1700069L16Rik 4:intron:Rnls 4:intron:1700112E06Rik 4:intergenic:Gm6586-Gm10482 4:intron:Apba1 4:intergenic:Gm6767-Cblb 4:intergenic:Gm25650-Gm23584 4:intergenic:Gm5839-Gm25440 4:intron:Arf2 1:intergenic:Crbn-Gm22418 4:exon:Gm6031 4:intron:Gpbp1 4:intergenic:Asz1-Gm15593 2:intergenic:Gm10642/Fam63b-Adam10 3:intergenic:Syt14-Gm23583 4:intergenic:Gm24248-Rybp 4:intergenic:Gm23239-Ntrk3 4:intron:E130114P18Rik 3:intron:Adsl 4:intergenic:Mctp2-Gm10294 4:intron:Csmd1 3:intergenic:Gm25754-Gm24555 4:intergenic:Gm20581-Gm20579 4:intron:Tex11 3:intergenic:Cdh26-Gm14314 4:intergenic:Gm13809-Gm13808 4:intron:Tmprss11e 4:intergenic:AI606473-Tyw3 4:intron:Gm15398 4:intergenic:Cadm2-Gm24681 4:intron:Hbp1 4:intergenic:Slc22a30-Gm6425 4:intergenic:RP23-18M12.1-Tinag 4:intergenic:Gm12007-Gm12008 3:intergenic:Prkar2b-Pik3cg 4:intergenic:Gm25460-Gm5263 4:intergenic:RP23-131O4.2-Gm25380 4:intron:Cyyr1 4:intergenic:Tenm2-Gm12128 4:intergenic:Gm5839-Gm25440 3:intron:Il18bp 4:intron:Lingo2 4:intergenic:Arap3-Pcdh1 4:intergenic:Slc25a38-Rpsa 4:intergenic:Gm22276-Gm9825 4:intergenic:Gm23943-4930558J18Rik 4:intergenic:Gm23385-Gm7803 4:intergenic:Rp1-Sox17 4:intron:Atrnl1 4:intron:St13 4:intergenic:Gm23180-Gm22977 1:intergenic:RP23-446B17.2-Pitx2 4:intergenic:Dmrta2-Elavl4 4:intergenic:Gm17173-Txndc16 4:intergenic:Gm24946-Plch1 4:intron:Prkcb 4:intergenic:Gm21820-AC132591.1 4:intron:Chn1 4:intron:Samsn1 4:intergenic:Slc22a16-Ddo 4:intergenic:Megf9-Gm11227 4:intron:Grb10 4:intergenic:Igfbp5-Tnp1 4:intergenic:Gm22597-Gm24514 4:intergenic:Col28a1-Mios 4:intergenic:Adra2a-Gpam 4:intergenic:Gm17091-Gm6455 3:intergenic:Gm8475-Shroom2 4:exon:Brdt 4:intergenic:Casp12-Pdgfd 4:intron:Vmn2r102 4:intergenic:Gm6594-Pkdcc 4:intron:Sobp 4:intergenic:Gm26000-Ophn1 4:intergenic:Nox3-Gm23186 4:intron:Bai3 4:intergenic:Fam189a1-Tjp1 4:intergenic:Gm22807-Gm23681 4:intron:Fstl5 3:intergenic:Gm5593-Vmn2r60 4:intron:Erg 4:intergenic:RP24-86O15.2-Gm10698 4:intergenic:D3Ertd751e-Gm25714 4:intron:Arhgap18 4:intergenic:Gm23041-Nav3 4:exon:D630029K05Rik 4:intron:Ctnna1 4:intron:Dpp10 4:intron:Nkain3 4:intergenic:Gm11225-Cdk5rap2 4:intron:Sel1l2 4:intron:Car10 4:exon:Zc3h12c/mmu-mir-6244 3:intergenic:Phyhipl-4930533K18Rik 4:intron:Atp8b3 4:exon:Rhoq/Pigf 4:intergenic:Prune2-Gcnt1 4:intron:Wdr25 4:intergenic:RP24-86O15.2-Gm10698 4:intron:Reln 3:intergenic:Fam184a-Man1a 4:intergenic:Gm13471-Gm13481 4:intron:Gm7173 4:intergenic:Gm22891-Gm24774 4:intergenic:Gm12638-Gm12637 4:intergenic:Gm23534-Gm22727 4:intergenic:Pld5-Gm26104 4:intergenic:Ggh-Nkain3 3:intron:Dlg1 4:intergenic:Gm23708-G6pd2 4:intergenic:Gm23059-Gm7745 3:intergenic:Alg14-Cnn3 4:intron:Aff2 4:intron:Khdrbs3 4:intergenic:Tlr2-D930015E06Rik 4:intron:Fez1 4:intron:4930448K12Rik 4:intergenic:Gm13483-Gm13486 4:intergenic:Gm23790-Gm5422 4:intron:Glra2 4:intergenic:Gm24963-Gm10742 4:intergenic:Ubtf-Slc4a1 4:intergenic:Cdh7-Cdh19 4:intergenic:Vmn2r81-Vmn2r82 4:intergenic:Mtss1-Gm5045 4:intergenic:Gm24376-Gm19980 4:intergenic:Ebf4-Gm14044 4:intergenic:Gm12448-Gm22505 4:intron:Grid2 4:intergenic:Ccdc178-Asxl3 4:intergenic:Gm14099-Gm14098 2:intergenic:Zic4-Gm26101 3:intergenic:Cnksr3-Ulbp1 3:intron:Fshr 4:intergenic:Gm23217-Gm24390 4:intergenic:Gm21834-Gm25102 4:intergenic:Sorcs3-AC116451.1 4:intergenic:Gm23892-Gm25382 4:intergenic:Prex2-A830018L16Rik 4:intron:Agl 4:intergenic:Gm22967-Dlgap1 4:intron:Atp10b 4:intergenic:Sec61b-NAMA_1 4:intergenic:Gm24720-Tshz1 4:intergenic:Htr2c-Gm24627 4:intergenic:Gm25123-1700047F07Rik 4:intron:Egfem1 4:exon:Gm7146 4:intergenic:Pcdhb1-Pcdhb2 Off-target primers show all... show less...
221 / fw	`CTTATGTTTGCACTAATCTG TGG` `.................... ...` Enzymes: Csp6I Cloning / PCR primers	54	85	53	26	68	88	0 - 1 - 4 - 14 - 139 0 - 0 - 3 - 0 - 5 158 off-targets	4:intergenic:Gm24297-Gm22911 4:intergenic:Gm7936-Gm24396 4:intergenic:4930511F01Rik-Snrpb2 4:intron:Naa35 4:intergenic:BC030500-Gm24847 4:intergenic:Gm22382-Gm22169 3:intron:Gpr98 4:intergenic:Gm25658-Atp2b1 1:intron:Rb1 4:intergenic:Vgll3-Gm22217 4:intergenic:Gm22308-Cbfa2t2-ps1 4:intergenic:Crtac1-R3hcc1l 4:intergenic:Gm9578-Gm22891 4:intergenic:Gm11835-Gm11814 4:intron:Farsb 4:intergenic:Gm22852-Spt1 4:intergenic:Gm15971-Dyrk1a 4:intergenic:Gm25094-Gm22312 4:intron:Cdc20b 4:intergenic:Gm23670-Mrps28 4:intergenic:Ccdc160-Gm14607 4:intergenic:Gm24963-Gm10742 4:intergenic:Dlg2-Gm23928 4:intron:Atp5f1 4:intron:Akap9 3:intergenic:Gm5614-Cdon 4:intergenic:Vax1-Kcnk18 4:intergenic:Hcrtr1-Tinagl1 4:intergenic:Fgf9-Gm25614 4:intergenic:Epha5-Gm24626 4:intergenic:Elfn1-Mad1l1 4:intron:Cpped1 4:intron:Ldlrad4 4:intron:Wdr52 4:intergenic:AI606473-Tyw3 4:intergenic:Gm25715-N6amt1 4:exon:Opa1 4:intergenic:Gm27043-Gm25020 3:intergenic:Gm8601-Btg1 4:intron:Cyfip1 4:intergenic:Gm16203-Sgcz 4:intron:Xpo7 4:intron:Kcnk10 3:intergenic:Add2-Tgfa 4:intergenic:Gm25133-Gm24770 4:intergenic:Gm24183-Gm22597 4:intergenic:Dnajc25-Gng10 3:intergenic:Gm23252-Olfr796 4:intergenic:Kit-Gm25374 2:intergenic:Ptgs1-Olfr334-ps1 4:intron:1700028P14Rik 4:intron:Flnb 4:intergenic:Gm22939-Cntnap2 4:intergenic:Sgcz-Gm23128 4:intron:Orc5 4:intergenic:Lemd3-Wif1 3:intron:Dock2 4:intergenic:Pabpc6-Qk 4:intergenic:Gm10750-Gm14135 2:intergenic:4930517O19Rik-Gm26367 4:intergenic:F930017D23Rik-Gm9034 4:intergenic:Ctif-1700034B16Rik 4:intergenic:1700018M17Rik-Hsdl2 4:intron:Col26a1 4:exon:Srpk2 4:intergenic:Gm24039-4930461C15Rik 4:intergenic:Gm23987-Fam135b 4:intergenic:Impg1-RP24-325L16.1 4:intergenic:Pabpc6-Qk 4:intergenic:Gm24632-Lyzl1 4:intergenic:Gm14516-Cypt1 4:intergenic:Rarb-Thrb 4:intergenic:Gm25336-Zfp648 4:intron:Arfgef1 4:intergenic:Arfgef1-Cpa6 4:intron:Gm15506 2:intergenic:Spef2-Gm26350 4:intergenic:Gm16227-A930011G23Rik 4:intergenic:Gli3-Inhba 4:intergenic:Rpsa-ps10-Mir3963 4:intergenic:Ccdc50-Fgf12 3:intergenic:Gm6564-AC158942.1 4:intron:Scube2 3:intron:Kcnn2 4:intron:Spata21 4:intergenic:Mir5099-Meox2 3:intron:Cdk14 4:intron:Gpr98 4:intergenic:Gm24697-Gm11404 4:intergenic:Gm22260-Bai3 4:intron:Zpbp 4:intron:Tle1 4:intergenic:Ldb2-4930431F12Rik 4:intron:Fam19a2 4:intergenic:Pla2g4a-7530420F21Rik 4:intergenic:Gm24350-Gm13464 4:intron:Scrn3 4:intron:Kcnn2 4:intergenic:Cyp7b1-Gm23686 4:intergenic:Klrg2-Clec2l 3:intergenic:Gm7485-Gm14926 4:intergenic:Gm5083-Gm27007 4:intergenic:Gm24146-Elfn1 4:intergenic:9430038I01Rik-Glrx3 4:intron:Ank2 4:intergenic:Gm26181-Triml1 2:intron:Sema5a 4:intergenic:Amd2-Hs3st5 4:intron:Paxbp1 4:intergenic:Gm25578-Actr3 3:intron:Fam19a5 4:intergenic:Gm7882-Gm5292 4:exon:Tle3 4:intergenic:Gm23385-Gm7803 4:intergenic:4631405K08Rik-Plxna2 4:intergenic:Gmds-Gm11381 4:intergenic:4933422H20Rik/Mir3968-Gm11695 4:intron:Sh2b3 3:intergenic:4930405D11Rik-Kif2b 4:intergenic:Gm23733-6530403H02Rik 4:intergenic:1700007K09Rik-Fam24a 4:intron:Kcnt2 4:intergenic:Tmem200c-Epb4.1l3 4:intergenic:H2afy2-Col13a1 4:intron:Smchd1 4:exon:Gm27002 4:intergenic:Gm4868-Gm23151 4:intron:Zfpm2 4:intergenic:Wnt5b-Erc1 3:intron:3010001F23Rik 3:intergenic:Gm8464-Gm7158 4:intron:A230006K03Rik 4:intergenic:Gm26928-Kcnn2 4:intergenic:Gm17068-mmu-mir-496b 4:intergenic:Gm24132-Gm25160 4:intron:Jakmip2 4:intergenic:Rad51b-Zfp36l1/Gm26669 4:intergenic:Mrgprx1-Mrgprb5 4:exon:Tbc1d2b 4:intergenic:Csmd3-Gm25007 4:intron:Exoc6b 4:intergenic:E230016K23Rik-Ccl6 4:intergenic:Fbxo8-Hand2 4:intergenic:Gm26327-Gm6851 4:intergenic:Gm22609-Lrrn2 4:intergenic:Gm12296/Dnah9-Shisa6 4:intergenic:Gm26119-Fzd8 4:intron:Kcnma1 4:intergenic:Pigu-Trp53inp2 4:intergenic:Gm1815-Zfp353-ps 4:intergenic:Slc24a3-BC039771 4:intergenic:Kcnh8-Efhb 4:intron:Zfpm2 4:intergenic:Fam110c-6030469F06Rik 4:intergenic:Gm13565-Gm13564 4:intron:2310035C23Rik 4:intergenic:Gm16160-Ngf 4:intergenic:Spata19-Opcml Off-target primers show all... show less...
565 / fw	`AAACCTAGCCAGGAGAAATA TGG` `.................... ...` Enzymes: XcmI Cloning / PCR primers	54	69	44	29	71	88	0 - 0 - 5 - 43 - 261 0 - 0 - 0 - 4 - 4 309 off-targets	3:intergenic:Gm25882-Gm6728 3:intergenic:Gm22269-Gm8177 3:intergenic:Nrsn1-Tpi-rs8 3:intergenic:Gm25803-Mpdz 3:intergenic:RP24-480M3.2-RP23-175E16.1 4:intergenic:Gm11404-Gm11221 4:intergenic:Gm8098-4930505A04Rik 4:intergenic:Gm5384-Chst7 4:intergenic:Gm26259-Pitrm1 4:intergenic:Tdrd3-Rps3a2 4:intron:4932441J04Rik 4:intergenic:Xkr4-Gm22848 4:intergenic:Col11a1-Gm23750 4:intergenic:Gm6913-Gm16261 4:intergenic:Hspe1-Gm16581 4:intergenic:Gm15663-Mettl25 4:intergenic:AW209491-Gm6556 4:intergenic:Fam84a-Gm9847 4:intron:Fhit 4:intergenic:Gm8730-Cdh5 4:intron:Sf3b1 3:intergenic:Gm13658-Gm13660 4:intergenic:Gm22342-Gm24584 4:intergenic:n-R5s60-Gm26256 3:intergenic:Gm24200-1190002N15Rik 3:intergenic:Gm25025-Gm25916 4:intron:Slc6a6 4:intergenic:Gm5973-Gm9915 4:intergenic:Stc2-Bod1 4:intron:2900053O20Rik 4:intergenic:Mir133b-Il17a 4:intron:Ptprn2 4:exon:Nek1 3:intergenic:Gm24839-Tmem132c 4:intergenic:Gm22839-Cntnap2 4:intergenic:Gm26177-Gm25602 4:intergenic:Sspn-Itpr2 4:intergenic:Gm7134-Gm9225 4:intergenic:Glrx3-Gm25798 4:intron:Suclg2 3:intergenic:Gm14795-Gm14792 4:intergenic:Emc2-Tmem74 4:intron:Rps6ka6 4:intron:Kif5c 3:intergenic:Gm2694-4933402J07Rik 4:intron:Ust 4:intergenic:Gm24446-Hmgb1-ps1 4:intergenic:Mir7b-Fem1a 4:intergenic:Gm24795-Gm8609 4:intron:Tm9sf4 4:intergenic:Gm22682-Col25a1 4:intergenic:Gm13925-Gm20638 4:intergenic:Auts2-Gm25722 4:intergenic:Htr1a-Dph3b-ps 4:intergenic:Gm23903-Ank 4:intron:Rsrc1 4:intergenic:Gm24734-Nog 4:intergenic:Fam110b-Gm11797 4:intron:Gm16462 4:intergenic:Gm12913-Gm25836 4:intergenic:Gm25513-Gm15975 4:intron:A830018L16Rik 4:intergenic:Gm23965-Gm23537 4:intergenic:Gm12649-Gm23443 3:intergenic:Sdc1-9930038B18Rik 3:intergenic:Gm14219-Gm11445 4:intergenic:Gm15913-Synpr 4:intergenic:Gm7113-Gm16465 4:intergenic:Gm25715-N6amt1 4:intergenic:Gm5919-Bckdhb 4:intergenic:Ofcc1-Gm9979 4:intergenic:Gm25432-Fosl1 4:intron:Nlgn1 4:intron:Fcrl1 3:intergenic:Gm23022-Rab28 4:intron:Cbll1 4:intergenic:Gm9871-Cntn3 4:intergenic:Gm12066-Gm12068 4:intron:Smyd3 3:intron:Spopl 4:intergenic:Gm25598-BC002059 4:intergenic:Gm13793-Gm26396 4:intron:Scamp1 4:intergenic:Gm24670-Etl4 3:intergenic:Gm22828-Gm23960 4:intergenic:Plcl1-Hsfy2 4:intergenic:Gm25434-Rergl 4:intergenic:Camk2b-Nudcd3 4:intron:Tmem145 4:intron:Rps6ka3 4:intron:Kif13b 4:intron:Kalrn 4:intergenic:Glrp1-Arl4c 4:intergenic:Ccser2-Rgr 4:intergenic:Gm24634-Gm17366 4:intergenic:Gm26288-Atp10a 4:intron:Esco2 4:intergenic:Rbm31y-Gm21904 4:intergenic:Gm24522-4930558G05Rik 4:intergenic:Gm21379-Gm21891 4:intergenic:Ncoa7-Gm24189 4:intergenic:Mfsd1-Iqcj 4:intergenic:Gm22828-Gm23960 4:intergenic:Emc2-Tmem74 4:intron:Xkr4 4:intergenic:Gm23987-Fam135b 4:intergenic:Gm21731-AF067061 4:intron:Sdccag8 4:intergenic:Gm21378-Gm20767 4:intergenic:Dleu2-Gm26969 4:intergenic:Gm21761-B020031M17Rik 4:intergenic:Gm22347-Gm23764 4:intergenic:Gm26291-Gm17377 3:intergenic:RP23-461P14.6-Prtg 4:intergenic:Gm24508-Gm25292 4:intron:Thap4 4:intron:Ncapd3 4:intergenic:Gm15168-Gm5764 3:intergenic:Gm26565/Celf2-Celf2 4:intergenic:Igkv1-117-Igkv2-116 4:intergenic:Gm7719-Gm24377 4:intergenic:Igkv1-110-Igkv2-109 4:intergenic:Gm10110-Gm25495 4:intergenic:Oacyl-Sec11c 3:intergenic:St6galnac3-AC102738.1 3:intergenic:Gm8100-Gm26795 4:intron:Cab39l 4:intergenic:Gm10087-Sbf2 4:intergenic:Zcchc9-Ckmt2 4:intergenic:4930596M17Rik-Lyzl4 4:intron:Lrrc4c 4:intergenic:Gm5392-Itm2a 4:intergenic:Gm25598-BC002059 4:intergenic:Fmnl3-Tmbim6 4:intergenic:Igf2bp2-Gm25810 4:intergenic:n-R5s209-Gm7846 4:intergenic:Gm26977-Pard6g 4:intergenic:Gm11259-Gm11256 4:intergenic:Egf-Lrit3 4:intergenic:Actr3-Slc35f5 2:intergenic:Pcdhga9/Gm26672-Pcdhga9 3:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Mrgpra3-Gm22427 4:intergenic:Gm23764-KCTD12/Gm26778/Kctd12/Mir5130 4:intron:4930467E23Rik 4:intron:Kansl2 4:intergenic:Ms4a4c-Ms4a4b 4:intergenic:Gm11732-Gm11733 4:intergenic:Gm24387-Foxp1 4:intergenic:Eif3s6-ps1-Gm11995 3:intergenic:Phlpp1-Bcl2/D630008O14Rik 4:intron:Tek 4:intergenic:Gm13125-Pramef12 3:intron:Cdh10 4:intergenic:Gm15668-Kif22-ps 4:intron:Gm9733 4:intergenic:Gm6931-Ift57 4:intergenic:Grpr-Rnf138rt1 4:intergenic:Gm5759-Gm8880 4:exon:Epn3 4:exon:Gm12380 3:intergenic:Gm5342-Gm23815 4:intron:Ralgapa1 4:intron:Agbl4 4:exon:Telo2 4:intergenic:Gm16722-Mecom 4:intron:Samd4 4:intergenic:Vmn1r-ps6-Samd9l 4:intron:Cacna2d3 4:intergenic:Atp1a1-Mab21l3 4:intron:2810055G20Rik 4:intergenic:4931417E11Rik-Gm26179 3:intergenic:Neto1-Cbln2 4:intergenic:Gm26748-Tpt1-ps3 4:intergenic:Eif3s6-ps2-Gm25418 4:exon:Lphn2 4:intergenic:Gadl1-Tgfbr2 3:intron:Dok6 4:intron:Pcdh9 4:intergenic:Gm22114-1700084C01Rik 4:intergenic:3425401B19Rik-1810011H11Rik 4:intergenic:Pik3cb-Gm6432 4:intron:Coro2a 4:intron:Cntn1 4:intron:Dcx 3:intergenic:Olfr582-Olfr583 4:intergenic:Rpl7a-ps10-Trim42/Gm15891 4:exon:Hspbap1 3:intron:Msi2 4:intergenic:Rspo3-Gm10275 3:intergenic:Guca2b-Hivep3 4:intron:Fry 4:intron:Focad 2:intergenic:1700001G01Rik-Gm26235 4:intergenic:Gm3867-Gm7257 4:intergenic:Gm7976-Kcnj15 4:intron:Xxylt1 4:intergenic:Mmp27-Mmp20 4:intergenic:Gm23015-4932441J04Rik 4:intergenic:Wasf1-Gpr6 4:intergenic:Ngf-2410057H14Rik 3:intron:Spata17 4:intron:Plscr1 4:intron:Zdhhc14 4:intergenic:Gm4258-Ptprc 4:intergenic:Gm23896-Gm24902 4:intergenic:Gm17384-3110047P20Rik/Gm9954 4:intron:Elmod1 3:intergenic:Gm9001-Gm22283 4:intergenic:Dusp7-Rpl29 4:intergenic:Gm23024-Gm24901 4:intergenic:Gm26171-Fam49a 4:intergenic:Gm13360-Etl4 2:intron:Gab3 4:intergenic:Mrgpra9-Mrgprx3-ps 4:intergenic:Ythdf3-Gm26485 4:intergenic:Gm8100-Gm26795 4:intergenic:Gm25454-n-R5s165 4:intergenic:1500009L16Rik-Nuak1 4:intergenic:Gm25650-Gm23584 4:intergenic:Gm26235-Gm25392 4:intron:Rhbg 4:intergenic:Gm12252-1810065E05Rik 3:exon:Tnrc6b 3:intron:Itgb6 4:intergenic:n-R5s51-Cldn10 4:intron:Gm26691 4:intergenic:Gm13550-Ccdc148 4:intergenic:Gm14913-Gm6491 3:intergenic:Pcdh17-Gm23926 4:intergenic:5730508B09Rik-Gm10650 3:intergenic:Gm26396-Gm13795 4:intergenic:4930547C10Rik-4930552N02Rik 4:intron:Actn2 3:intron:Csmd1 3:intergenic:Gm8888-Slc38a4 4:intergenic:Gm14657-Gm8260 4:intron:Epb4.1l4b 3:intron:Sorcs1 3:intergenic:Gm24404-Nrg3 4:intergenic:Tcstv1-Gm21761 4:intergenic:Mdfic-Tfec 4:intergenic:Cts6-Cts8 4:intron:Dph6 3:intergenic:Gm24790-Klf6 4:intron:Dcp1a 4:intron:Opn1sw 4:intergenic:B230323A14Rik-Bnip2 4:intergenic:Dsg3-Dsg2 4:intergenic:Gm5257-mmu-mir-6343 4:intergenic:Gm24723-6720483E21Rik 2:intron:Slit1 4:intergenic:Olfr578-Olfr582 4:intergenic:Ednrb-RP24-312G4.2 4:intron:Cmip 4:intergenic:Cyp3a57-Cyp3a16 4:intergenic:4930510E17Rik-Exph5 4:intron:Baiap2 4:intron:Ercc3 4:intergenic:5430435G22Rik-Slc26a9 4:intergenic:Rpl21-ps4-Gm3839 4:intron:Usp13 4:intron:Vipr1 4:intergenic:Ahr-Agr3 4:intergenic:Gm23896-Gm24902 4:intergenic:Slc35f3-Coa6 4:intron:Parm1 4:intron:Ccdc178 4:intron:Fam114a1 4:intergenic:Tex15-Ppp2cb 4:intergenic:Olfr477-Olfr478 2:intergenic:Bdh1-Gm26053 4:intron:Spred2 4:intergenic:Gm22227-Zfp703 3:intergenic:Gm11263-Gm11409 4:intron:Myo6 4:intergenic:9430020K01Rik-Gm10556 4:intergenic:Gm14660-Sox3 4:intron:Shc3 4:intergenic:Emx2-Gm17203 4:intron:Frem2 4:intergenic:Ipmk-Gm22937 4:intergenic:Gm20767-Gm21818 4:intergenic:mmu-mir-6356-4930546C10Rik 4:intergenic:Gm15166-Cypt3 3:intergenic:Olfr145-Olfr878 4:intron:Cpq 4:intergenic:Gm26406-Gm23768 4:intergenic:Wipi2-Slc29a4 4:intron:Dlgap2 4:intergenic:Gm5432-Lpin1 3:intron:Qsox1 4:intergenic:BC048403-Srgap1 4:intergenic:Wnt5b-Erc1 4:intergenic:Gm4954-Gm6286 4:intron:Ttn 4:intergenic:n-R5s30-Rbfox1 4:intron:Dysf 4:intron:Pik3ap1 4:intron:Mib1 4:intron:Sirpb1b 4:intergenic:Bet1-RP23-447L9.1 4:intergenic:Gm6851-Gm24910 4:intergenic:Gm9081-Avpr1a 4:intergenic:Rarb-Thrb 4:intergenic:Duxbl3-4933413J09Rik 4:intergenic:Gm13750-Gm25748 4:intergenic:T-Pde10a 4:intergenic:Gm24035-Cdh13 Off-target primers show all... show less...
39 / rev	`GGGAAGATTTTAAAGACAAG GGG` `.................... ...` Not with U6/U3 Cloning / PCR primers	52	69	63	37	68	81	0 - 0 - 7 - 45 - 345 0 - 0 - 1 - 7 - 7 397 off-targets	3:intergenic:Zfp516-Gm24720 3:intergenic:Snx7-Gm26871 4:intergenic:A2m-Mug1 4:intergenic:Tceal6-Gm15022 4:intergenic:Gm26861-Gm25496 4:intergenic:Gpr116-Mep1a 4:intergenic:Gm12158-Ebf1 4:intergenic:Gm23727-Gm20594 3:intergenic:Gm8730-Cdh5 4:exon:Olfr401 4:exon:Olfr410 4:exon:Olfr402 4:intron:Iqgap2 4:intergenic:Gm22360-Gm24586 3:intergenic:Gm15359-Gm6483 4:intron:Gmds 4:intron:Kcnh8 4:intergenic:Gm24921-Fscb 4:intergenic:Gm22211-Gm6181 3:intergenic:Gm23059-Gm7745 4:intergenic:Galr1-Mbp 3:intergenic:Gm8526-Edil3 3:intergenic:Gm23296-Akap6 4:exon:Usp40 4:intergenic:4931440J10Rik-Gm26536/Wdfy2/mmu-mir-8098 2:intron:4930545L23Rik 3:intron:Sspo 4:intergenic:Tcerg1l-Mapk1ip1 4:intron:Hpgd 4:intron:2610021A01Rik 4:intergenic:Gm14616-Gm14444 4:intergenic:Gm22603-Gria1 3:intron:Pon2 4:intergenic:Gm14601/Gm15482-Gm14644 3:intron:Lrch2 4:intron:Alg8 3:intergenic:Stmn4-Gm23899 4:intergenic:Ptx4-Clcn7 3:intron:Phactr3 3:intergenic:Gm26580/Kcnq5-Rims1 4:intergenic:Gm15220-Gm15218 4:intron:Fbxw7 4:intergenic:Ndfip2-4930449E01Rik 4:intergenic:Prkcq-Gm13293 4:intergenic:Gm9843-Usp25 4:intron:Lrba 4:intergenic:Nrxn3-AC171335.1 4:intron:Ephx2 4:intergenic:Gm5161-Ephb1 4:intron:Pde4b 3:intergenic:Gm12004-4930554G24Rik 4:intron:Zfp948 4:intron:Sacs 4:intergenic:Gm25258-Hao1 4:intergenic:Cntnap5c-Gm5815 4:intron:Arhgap42 4:intron:Gpc6 4:intron:Neil3 4:intron:Myo18a 4:intergenic:Sphkap-Gm25754 3:intergenic:Gm22005-Nmt2 4:intergenic:Stk31-Npy 4:intergenic:Gm22202-Tmem106c 4:intergenic:Gm24295-Mef2c 4:exon:Pou1f1 4:intron:Endou 4:intergenic:Gm8705-Klf10 4:intron:Man1a 4:intergenic:RP24-456G3.2-RP24-456G3.1 4:intron:Unc13c 4:intron:Dock1 3:intron:Atp6v1h 4:intergenic:Tmprss15-Rps19-ps12 4:intergenic:Gm9867-Gm13016 4:intergenic:Galnt13-Gm25489 4:intergenic:Gm23980-Gm22810 3:intergenic:Gm15785-Bicd1 4:intergenic:Wfs1-Jakmip1 4:intergenic:Gm8730-Cdh5 4:intergenic:mmu-mir-6336-Lrp1b 4:intergenic:Adamts5-Gm25715 3:intergenic:Gm24248-Rybp 4:intergenic:Hmgb1l-Tas2r130 4:intergenic:Spaca3-Asic2 4:intergenic:Gm9381-Ndst4 3:intergenic:Faim2-Gm5808 4:intergenic:Reep5-Pkd2l2 4:intergenic:4930401G09Rik-Gm13832 4:intergenic:Gm14330-Grin2b 4:intron:Rcan2 4:intron:Rbfox3 4:intron:Rit2 4:intron:Cdh2 4:intergenic:Gm24278-Olfr958 4:intron:Hunk 4:intergenic:Rps19-ps12-Gm23083 4:intron:RP23-304I16.1 4:intergenic:Gm5614-Cdon 4:intron:Coro1c 4:intergenic:Gm8793-Nkain2 4:intergenic:Tmem167-Gm21726 4:intergenic:Gm26474-RP24-351J24.2 4:intergenic:Gm4876-D830026I12Rik 4:intergenic:Scd1-Wnt8b 4:intergenic:Gm7536-Gm24704 4:intergenic:Scn11a-Gm2449 4:intergenic:Smim20-n-R5s172 4:intron:Megf11 4:intergenic:Acot10-Gm25711 4:intergenic:Olfr510-Olfr512 4:intergenic:Plekhg1-Gm16149 4:intergenic:Gm20516-Gm21947 4:intron:Lypd6b 4:intergenic:5033428I22Rik-Scrg1 4:intergenic:Gm24815-Gm9144 3:intergenic:4921524J17Rik/1700051O22Rik-Gpt2 4:intron:Hdac9 4:intergenic:Gm24382-Mbnl1 3:intron:Col26a1 4:intergenic:Rcbtb2-Rcbtb2/Rb1 4:intergenic:Gm10710-Gm22342 4:intergenic:Epha5-Gm24626 4:intergenic:Gm25715-N6amt1 4:intergenic:Nlrp4f-Gm10775 4:intergenic:Fbxo15-Gm25453 4:intron:Itpr1 4:intron:Gpm6b 4:intron:Tab3 4:intergenic:Bmper-Npsr1 4:intergenic:Gm2310-Gm2348 4:intergenic:Gm15762-B230104C08Rik 4:intron:Bcor 4:intron:Bcl11a 4:intergenic:Ppp1r2-ps7-Gm26131 4:intergenic:Gm6644-Plcl1 4:intron:Dnah10 4:intergenic:Bche-Gm6098 4:intergenic:Gm22211-Gm6181 3:intergenic:Gm23067-Gm26072 4:intergenic:Tmem45b-Barx2 4:intron:Rnf144b 4:intergenic:Gm6098-Zbbx 4:intergenic:Gm22469-Gm23909 4:intron:Ccdc109b 3:intergenic:Gm25658-Atp2b1 4:intergenic:Gm14448-Gm14450 4:intron:Cntn1 4:intron:Slco5a1 4:intron:Aff3 4:intergenic:Tmem45b-Barx2 4:intergenic:Gm22547-Gm8919 4:exon:Gm13263 3:intergenic:Gm13988-Sema6d 4:intron:Gpc6 4:intron:Chchd3 3:intron:Med8 4:intron:Samd12 4:intergenic:Gm14876-Gm6325 4:intron:Megf11 4:intron:Ccser1 4:intergenic:A530020G20Rik-A730020M07Rik 3:intergenic:Defb41/Gm15386-Gm24075 4:intergenic:Gm24541-Zfp710 4:intergenic:Gm20713-Gpc5 4:intron:Tmprss6 4:intergenic:Epha4-mmu-mir-6352 4:intergenic:Gm23769-Gm23203 3:intron:Aoah 4:intergenic:Aig1-Gm22995 4:intergenic:Tfb1m-Nox3 3:intergenic:Gm22603-Gria1 4:intergenic:Gm22538-Bche 4:intergenic:Gm10650-Gm22293 4:intergenic:Gm4219-E330013P04Rik 4:intron:Slc26a7 4:intergenic:4930511F01Rik-Snrpb2 4:intron:Prkg1 4:intergenic:Stxbp5-Gm25682 4:intergenic:Gm26475-Gm24522 4:intergenic:Gnb4-Actl6a 4:intron:Ikzf1 4:intron:Kcnq1 4:intergenic:Gm8116-Col12a1 4:intergenic:4930564D02Rik-1700095B22Rik 4:intergenic:Gm12371-Gm12372 4:intergenic:Slc22a15-Nhlh2 4:intergenic:Islr-Islr2 4:intergenic:1700029P11Rik-Xrcc6 4:intergenic:Gm5764-Gm15185 4:intron:Rnf150 4:intergenic:CT030684.1-Gm23276 4:intergenic:Fam19a2-Gm23777 4:intergenic:Gm25006-Gm8858 4:intron:Ndufs2 4:intergenic:Gm7931-Slit2 4:intergenic:Gm10650-Gm22293 4:intergenic:Agpat9-Gm20484 4:intergenic:4930448E22Rik-Gm22659 4:intergenic:Gm16477-Gpr26 4:intergenic:AC132444.5-AC132444.2 4:intergenic:AC125149.1-AC125149.2 4:intergenic:AC133103.6-AC133103.7 4:intron:Kank4 4:intergenic:Sntb1-Has2 4:intergenic:Gm10295-Mctp2 4:intergenic:Slc30a10-Lyplal1 4:intergenic:Gm5593-Vmn2r60 4:intergenic:Gm24433-Eea1 3:intron:Erc1 4:intron:Col6a6 4:intron:Nubpl 4:intron:Magi2 4:intergenic:Nup37-Ccdc53 4:intergenic:Gm9726/mmu-mir-8099-2-Gm26055 4:intergenic:Gm14534-5730405O15Rik 4:intergenic:Olfr495-Olfr497 4:exon:Gm14455/Rtfdc1 3:intergenic:Gm24935-Gm21232 4:intergenic:5830454E08Rik-Gm23624 4:intergenic:Csnk2a2-Ccdc113 4:intergenic:Gm13377-Gm13376 4:intergenic:Gm10287-Gm26468 4:intron:Proser1 3:intergenic:Gm6462-Gm25586 4:intergenic:Gm23591-Speer2 4:intergenic:Gm11682-Kcnj16 4:intergenic:1700025D23Rik-1700001J04Rik 4:intergenic:D3Bwg0562e-4833424O15Rik 4:exon:Creb3/Gba2 4:exon:Gpr26 4:intron:Ankrd17 4:intergenic:Gm11410-Gm11406 4:intron:Qsox1 4:intergenic:Slc25a30-Gm4285 3:intergenic:Gm12913-Gm25836 4:intron:Armc1 4:intergenic:Zfp516-Gm24720 4:intron:Dock1 4:intron:Fras1 4:intergenic:Gm4991-Gm7134 4:intergenic:Gm23764-KCTD12/Gm26778/Kctd12/Mir5130 4:intergenic:1700001G01Rik-Gm26235 4:intergenic:Olfr402-Olfr403 3:intergenic:Gm11258-Gm11253 4:intergenic:Tmem28-Eda 4:intergenic:Gm24595-Maged1 3:intergenic:3830408C21Rik-Apoo-ps 4:intergenic:Gm20516-Gm21947 4:intergenic:Gm24490-Abcc12 4:intergenic:Nrsn1-Tpi-rs8 4:intergenic:Gm23780-C130074G19Rik 4:intergenic:Slc22a6-Chrm1 4:intergenic:Plod2-Gm9621 4:intergenic:Usp-ps-Olfr659 4:exon:Olfr658 4:intergenic:Olfr502-Olfr503 4:intron:A730090N16Rik 4:intergenic:Gm5046-Gm23907 4:intron:Taar2 4:intergenic:Gm15496-Kcnmb2 4:intergenic:Gm27023-Gm25800 4:intron:Sez6l 4:intergenic:1700034B16Rik-Zbtb7c 4:intergenic:Gm10295-Mctp2 3:intron:Uvrag 4:intron:Cdk8 3:exon:Bola3 4:intron:Trpc6 2:intergenic:Msr1-Fgf20 3:intergenic:Med13l-n-R5s175 4:intergenic:Gm7847-Gm23674 4:intergenic:Gm12680-Sh3gl2 4:intergenic:Ptgs1-Olfr334-ps1 4:intron:Ccdc91 4:intron:Pja1 4:intergenic:Gm25550-Cdh9 4:intergenic:Gm9843-Usp25 4:intergenic:Plk2-Actbl2 4:exon:Nlrp9b 4:intergenic:Cdk15-Fzd7 4:intron:Lrrc4c 4:intron:Slc27a6 3:intron:Anks1b 4:intergenic:1110059M19Rik-Gm14606 4:intergenic:Gm25275-Gm24274 2:intergenic:Fhod3-Tpgs2 4:intron:1700039E22Rik 4:exon:Ces2e 4:intergenic:Gm10549-2410004N09Rik 3:intergenic:Tnfaip8l3-Cyp19a1 4:intergenic:Awat2-Igbp1 4:intron:Stard13 4:intron:Dmxl2 4:intergenic:Gm24092-Cenpf 4:intergenic:Pvrl3-Gm6912 4:intergenic:Dsc3-Dsc2 2:intergenic:Snap25-Gm14053 3:intergenic:Kcnh7-Gm23503 4:intergenic:Gm26489-1700028P15Rik 4:intergenic:Csmd1-Gm25665 4:intergenic:Ccdc82-Phxr4 4:intergenic:Gm97-Gif 4:intergenic:Gm14505-Gm9435 3:intron:Ccdc138 4:intron:2610034M16Rik 4:intron:Ccdc178 4:intergenic:Gm23558-Galc 2:intron:Bcl2l1 4:intergenic:Il33-Gm26046 4:exon:1010001N08Rik 4:intergenic:Gm15913-Synpr 4:intron:Pde4d 4:intron:Atp11b 4:intergenic:Btrc-Gm6807 4:intergenic:Gm11609-2310016D23Rik 4:intergenic:Gm12710-Wdr78/Mier1 4:intron:Pde4b 4:intron:Map3k9 4:intergenic:Gm25598-BC002059 4:intron:Ltv1 4:intergenic:Gm26341-Amd2 4:intergenic:Gm13942-Gm13941 4:intron:Syt1 4:intron:Bmp2k 4:intergenic:Gap-Gm16088 4:intergenic:Gm8888-Slc38a4 4:intergenic:Gm26933-Tnfrsf11b 4:intergenic:Gm7936-Gm24396 4:intergenic:Gm22976-Ppargc1a 4:intergenic:Tmpo-Rmst 4:intergenic:Nctc1-H19 4:intergenic:Gm94-Spink5 4:intron:Cubn 4:intron:Fgd4 4:intron:Sestd1 2:intergenic:Gm25656-Cntn6 4:intergenic:Pabpc2-Yipf5 4:intron:Fam151a 4:intergenic:Gm23162-Runx1t1 4:intergenic:Gm21820-AC132591.1 4:intron:Slx4ip 2:intergenic:Gm23777-Slc16a7 4:intron:Cog8 4:intergenic:Vmn1r-ps17-Gm20718 4:intergenic:AC163993.1/Slc1a1-Slc1a1/AC163993.1 4:intergenic:Fam98a-Gm24126 4:intergenic:Gm11984-Adcy1 4:intergenic:Atp13a4-Opa1 4:intergenic:Crabp1-Ireb2 4:intron:Cdh13 4:intergenic:Gm23591-Speer2 4:intergenic:Gm4409/Lrrtm4-Lrrtm4 3:intergenic:Gm23795-Gm5342 3:intergenic:Letm1-Gm10059 4:intron:Astn2 4:intron:Gm831 4:intergenic:Gm13446/Dab2ip-Dab2ip 4:intergenic:Cadm2-Gm24681 4:intergenic:Rpl28-ps4-Gm9946 4:intron:Fiz1 4:intergenic:Paqr5-Glce 4:intron:Npas3 4:intron:Slc38a9 4:intron:Chmp4b 4:intergenic:Gm26468-Rpsa-ps10 4:intergenic:6530403H02Rik-Rwdd3 4:intron:Ints10 4:intergenic:Gm25176-Smarca2 4:intergenic:AC121988.1-Gm25740 3:intron:Fto 3:intron:Slc13a1 4:intergenic:Gm25601-Sod3 4:intergenic:Kcnip4-Gm20700 4:intron:Armc9 4:intergenic:Gm11501-Gm25113 4:intron:Ube2e3 4:intergenic:Perp-Tnfaip3 4:intergenic:Gm22506-RP23-448C3.1 4:intergenic:Tfb1m-Nox3 4:intron:Fcrl5 4:intron:Tcf4 4:intergenic:Gm12519-Gm12520 4:intergenic:Gm3756-Arhgap32 4:intron:Gm4409 4:intron:Runx1 4:intergenic:Wbscr17-Auts2 4:intron:Glb1l3 4:intron:Layn 4:intergenic:Gm26297-Pvrl3 4:intergenic:Mki67-Gm9341 4:intergenic:St5-Akip1 4:intergenic:Emx2os-Emx2os/Emx2 4:intergenic:Gm14619-Sh2d1a 4:intergenic:Gm24591-Gm26241 4:intergenic:Iqca-Ackr3 4:intergenic:Acpp-Mir2136 4:intergenic:Gm379-Brwd3 Off-target primers show all... show less...
94 / fw	`CCATGATTGCTCAGGATTGT TGG` `.................... ...` Inefficient Cloning / PCR primers	52	86	41	18	67	71	0 - 1 - 1 - 14 - 122 0 - 0 - 0 - 1 - 1 138 off-targets	1:intron:Acvr1 4:intergenic:Olfr1463-Gm22443 4:intergenic:Olfr1465-Olfr1466 4:intergenic:Gm9578-Gm22891 4:intergenic:Gm24531-Gm5537 4:exon:Runx3 4:exon:Mycs 4:intergenic:n-R5s209-Gm7846 4:intergenic:Gm23514-5730522E02Rik 4:intergenic:Gm23240-Gm8357 3:intron:Fbxo18 4:intergenic:AC124326.1-Mir802 4:intergenic:A730045E13Rik-Gm26714 4:intergenic:Adam30-Reg4 4:intron:Abcg1 3:intergenic:Gm11922-Map3k7 4:intergenic:9130204L05Rik-Gm23697 4:intergenic:Gm16685-Gm24614 4:intergenic:Arl13a-Nox1 4:intergenic:Gm24987-Socs6 4:intergenic:Mylk3-4921524J17Rik 4:intergenic:Cbln2-Gm5096 3:intergenic:Dmtf1-Gm24666 4:intron:Ano6 4:intergenic:Gm9931-Gm22966 4:intergenic:Gm8993-Gm9001 4:intergenic:Ntrk2-Agtpbp1 4:intergenic:Areg-Gm23092 4:intron:Rgs7 4:intergenic:Chd9-Gm22428 4:intron:Cntnap5a 4:intergenic:Gm24861-Chodl 4:intron:Gm14010 4:intron:Gm26843 4:intron:S100z 4:intron:Top1 4:intergenic:Gm22797-Robo1 4:intergenic:Fam20b-Gm15428 4:intergenic:Gm23514-5730522E02Rik 4:intergenic:AC116451.1-Rpl13a-ps1 3:intron:Col16a1 4:intergenic:Mnat1-Gm23910 4:intergenic:Epn2-Grap 4:intron:Ak5 4:intergenic:Gm15606-Opcml 4:intergenic:4933439G12Rik-Pip4k2a 3:exon:Tango2 4:intron:Tpd52 4:intergenic:Ptn-Dgki 4:exon:Tctn3 4:intergenic:Trio-Dnah5 4:intergenic:Gm24587-Dcn 4:intergenic:Gm16050-Gm8603 4:intergenic:Unc93a-Smok2a 4:intergenic:Gm26651-Diras2 4:intergenic:Gm24720-Tshz1 4:intron:Sorcs1 4:intergenic:F630028O10Rik/Mir223-Vsig4 4:intergenic:Magi1-Slc25a26 3:intergenic:Ubqln1-Gkap1 4:intergenic:Banf2-Gm12357 4:exon:Ndc1 4:intron:Pde1b 4:intergenic:Gm23980-Gm22810 4:intergenic:4930556L07Rik-Gm14153 4:intergenic:Gm11279-Gm11280 4:intron:Gm13481 4:intron:Ccdc55 4:intron:Stambpl1 4:intergenic:1190007I07Rik-Tdg 4:intergenic:mmu-mir-6401-Gpr123 4:intron:Nav1 4:intergenic:Gm22104-Gm25550 4:intron:Lsamp 4:intron:Stam 4:intergenic:Ntng1-Prmt6 4:intergenic:Gm24248-Rybp 4:exon:Cntn4 4:intergenic:Rps16-ps3-3930402G23Rik 4:intergenic:Clcnkb-Hspb7 4:intergenic:Sorl1-Sc5d 4:intergenic:Gm11680-BC006965 4:intron:Kremen1 4:intergenic:Agtr1b-Gm7488 3:intergenic:Gm14569-Pgrmc1 3:intron:Akap14 4:intergenic:Gm15405-Gm27039 4:intergenic:Wisp3-Fyn 4:intergenic:Veph1-Shox2 4:intergenic:Gm23831-1700017G19Rik 4:intergenic:Zfp516-Gm24720 4:intron:Vti1a 3:intergenic:Ube2ql1-Med10/Gm26819 4:intergenic:Tbc1d23-Tmem30c 4:intergenic:Gm11581-Ctla4 3:intron:Zmat4 4:intron:Gabrg3 3:intergenic:Gm22326-Sv2b 4:intergenic:Gm7731-Gm21897 4:intergenic:Gm22628-5033411D12Rik 4:intergenic:Cacul1/Gm25238-Nanos1 4:intergenic:BC049702-Gm14531 4:intergenic:Gm525-Gm11497 4:intron:Ptprk 4:intergenic:Akirin1-Rhbdl2 4:intergenic:Dock9-Ubac2 4:intergenic:Gm13816-Gm13791 4:intergenic:Gm9578-Gm22891 3:intergenic:Olfr721-ps1-Slc4a7 4:intron:Hspg2 4:intergenic:Gm25799-Slit3 4:intergenic:Gm12034-Sertad2 2:intergenic:Gm7224-Vmn2r59 3:intergenic:Gm2308-Vmn2r60 3:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Gm6584-Gm15861 4:intergenic:Gm25258-Hao1 4:intergenic:Nkx1-1-Fam53a 4:intergenic:1600002D24Rik-Psmg1 4:intergenic:Gm23733-6530403H02Rik 4:intergenic:Gm14670-Gm14675 4:intron:Pik3r5 4:intergenic:Krt6b-Krt6a 4:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:Gm10710-Gm22342 4:intergenic:Cask-Gpr34 4:intron:Dgki 4:intergenic:Gm13393-Gm13396 4:intron:Cacna2d1 4:intergenic:Fam76b-Sesn3 4:intergenic:Rbbp8-Gm6277 4:intergenic:Gm17538-Gm5229 4:intergenic:Gm15204-Reps2 4:intron:Gm12132 4:intergenic:AC112986.1-Tsn 4:intron:Kcnj6 4:intron:Ptn 4:intergenic:Sqrdl-Gm13989 Off-target primers show all... show less...
324 / rev	`CATGCATTTCACTATGAAAA TGG` `.................... ...` Enzymes: TspDTI Cloning / PCR primers	52	72	38	28	71	64	0 - 0 - 12 - 37 - 234 0 - 0 - 4 - 4 - 5 283 off-targets	4:intergenic:Gm12728-Gm24363 2:intergenic:Grm6-Zfp454 4:intergenic:Vmn2r17-Gm8488 3:intergenic:Gm26895-1700123M08Rik 3:intron:9130230L23Rik 2:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Adra2a-Gpam 4:intron:Otop1 4:intron:Hectd2 2:intergenic:Ceacam5-Gm6065 3:intergenic:Cfh-Kcnt2 4:intergenic:D630045J12Rik-Zc3hav1l 4:intergenic:Gm8100-Gm26795 3:intergenic:Gm5127-Gm14879 3:intergenic:Gm7224-Vmn2r59 4:intergenic:A530076I17Rik-Gm15516 2:intergenic:Gm15581-Gm25021 3:intergenic:4930529F24Rik-Nrg3 4:intergenic:Agfg1-Slc19a3 4:intergenic:Gm26119-Fzd8 4:intergenic:Epha3-Csnka2ip 4:intergenic:Kcng4-Wfdc1 4:intergenic:Pnmal1-Ccdc8 3:intergenic:Gm25248-Gm26326 4:exon:Clca2 4:intergenic:Gm7481-Prkd1 3:intergenic:Gm22618-Gm22976 4:intergenic:Gm22944-RP23-442I7.1 4:intergenic:Gm9002-Kifc5c-ps 4:intergenic:Gm21614-Gm23249 4:intron:Micu1 4:intron:Myo9a 3:exon:Olfr47 4:exon:Olfr434 4:intron:Rap1a 4:intergenic:Gm26771-Tlr2 4:intergenic:Sgcd-Gm23039 4:intron:Cntnap2 4:intron:Ddr2 4:intergenic:Mir1961-4932430I15Rik 4:intergenic:Insig1-En2 4:intron:Dock4 4:intergenic:Gm23709-Unc13b 4:intron:E430025E21Rik 4:intergenic:Gm8730-Cdh5 4:intergenic:Ralb-RP23-386K20.2 4:intergenic:Atp6v1g3-Nek7 3:intergenic:Slc35f5-Gpr39 4:intron:Dimt1 4:intergenic:Appl2-1500009L16Rik 4:intron:Tmem231 4:intergenic:Efna5-Fbxl17 4:intergenic:Cables1-Tmem241 4:intron:Snw1 4:intron:Phka1 2:intron:Mecom 4:intergenic:Ipmk-Gm22937 4:intergenic:Gm23260-Nr3c2 4:intergenic:Gm6931-Ift57 4:intergenic:Gm11674-Gm11679 4:intergenic:1700112E06Rik-Gm5670 4:intergenic:Amd2-Hs3st5 4:intergenic:Gm15286-Mier3 4:intergenic:Gm12421-Bnc2 4:intergenic:Ap3b1-Tbca 4:intron:Gm11884 4:intergenic:Gm6505-1700112D23Rik 4:intergenic:Cntnap5a-Gm23393 4:intron:Slc9a9 4:intergenic:Bsph1-RP23-417L14.1 4:intergenic:Gm9621-Gm24200 4:intron:Lrp1b 4:intron:Ube2e3 4:intergenic:Gm4916-Gm26475 4:intron:Cntn6 4:intron:Gm14066 4:intergenic:Rpsa-ps10-Mir3963 3:intergenic:Gm5839-Gm25440 4:intergenic:Gm22088-Rps11-ps4 4:intergenic:Gm25101-Cdh12 4:intron:Cntnap2 4:intergenic:Sntb1-Has2 4:intergenic:Gm10797-Zfp365 4:intergenic:Ypel5-Lbh 3:intergenic:Epha5-Gm24626 4:intron:Wdfy3 4:intergenic:Gm25670-Gm26255 4:intergenic:Gm12897-Gm12893 4:intergenic:Gm10220-Gm7361 4:intergenic:Gm25078-Gm14653 4:exon:Rnf213 4:intergenic:Gm4559-Gm10013 4:intergenic:Gm23154-Gm12604 4:exon:Mysm1 2:intergenic:Fgf10-Nnt 4:intron:Frmd4a 4:intron:Prdm2 4:intergenic:Gm10571-Gm23845 4:intron:Nmnat2 4:intergenic:Igkv9-120-Igkv1-117 4:intergenic:Gm7488-Tbl1xr1 4:intergenic:Gm13518-mmu-mir-195b 4:intron:Eif4g3 4:intergenic:Plk2-Actbl2 4:intron:Dip2b 4:intergenic:Stmn4-Gm23899 4:intergenic:Gm6634-Gm8604 4:intron:Itgbl1 4:intergenic:Gm5940-Gm8806 4:intergenic:1700084J12Rik-Cpq 4:intron:Gm4832 3:intergenic:Gm21614-Gm23249 4:intergenic:4930548G14Rik-Gm23665 4:intergenic:Dhx35-Gm25090 4:intron:Zfp831 4:intergenic:Gm12725-Gm12724 3:intergenic:Bbs9-U4 4:intergenic:Muc6-Muc2 3:intergenic:Dio3-Ppp2r5c 4:intron:Acoxl 3:intergenic:Gm12292-Elac2 4:intergenic:Gm10048-Kcnip4 4:intron:Ntrk3 4:intergenic:Gm15578-Negr1 4:intergenic:Gm22302-Uri1 4:intergenic:Gm22538-Bche 4:intron:Gfra2 4:intron:Kidins220 4:intergenic:Pot1a-Gm24691 3:intron:Trpc4 4:intergenic:Dhrs3-Vps13d 4:intergenic:Gm23987-Fam135b 4:intron:Tcf4 3:exon:Fhdc1 4:intron:Nbas 3:intergenic:Gm7627-Gm24120 4:intergenic:Gm25715-N6amt1 4:exon:Nedd1 4:intron:Abtb2 3:intron:Stxbp4 4:intron:Ctnna2 3:intergenic:Gm12911-Brinp1 4:intron:Mfsd7c 4:intron:Me3 4:intergenic:Gm26177-Gm25602 4:intron:Arhgef6 4:intergenic:Parm1-Gm5558 3:intergenic:Gm10192-Gm12696 4:intergenic:Sorcs3-AC116451.1 4:intergenic:Gm26018-BC018507 4:intergenic:Gm23986-Gm24929 4:intergenic:Gm12586-Gm12590 4:intron:Skint5 4:intergenic:4931417E11Rik-Gm26179 4:intergenic:6530411M01Rik-Gm8492 4:intergenic:Gm25537-Gm24122 4:intergenic:Sox2ot-mmu-mir-6378 4:intergenic:Ankfn1-Pctp 4:intergenic:Rgs17-Gm10945 3:intergenic:1700122O11Rik-1700067P10Rik 4:intron:Lrp4 3:intergenic:Btnl10-Rnf187 4:intergenic:Gm24285-4930467K11Rik 4:intergenic:Rragb-Gm15153 4:intron:Anxa7 3:intergenic:Gm23535-n-R5s218 4:intergenic:Gm22016-Cdc73 3:intergenic:Gm11498-Gm22702 4:intron:Adcy1 4:intergenic:Fgf9-Gm25614 3:intron:Btbd11 4:intergenic:Gm26691-Cxxc4 4:intron:6430548M08Rik 4:intergenic:Gm23625-Mad2l1 4:intergenic:Gm24691-Grm8 4:intron:Foxp2 4:intergenic:Gm5185-Gm24921 2:intron:Diap2 2:intergenic:Gm23061-Ostf1 4:intergenic:Gm23520-Gm12131 4:intergenic:Trgj3-Tcrg-C3 4:intergenic:Gm16248-Gm22668 4:intergenic:Kcnt2-Gm4845 4:intron:Dnajc6 4:intergenic:1700009N14Rik-Gm12385 4:intron:Aim2 4:intergenic:Fam134b-Zfp622 3:intron:Nr5a2 4:intergenic:mmu-let-7j-Gm6214 4:intergenic:Mical3-Pex26 4:intron:Gtf2i 4:intergenic:n-R5s32-Gm26562 4:intron:Glud1 4:intron:Snx13 4:intergenic:Gm15206-Reps2 4:intron:Fbxl20 4:intergenic:Mir466f-4-Irx1 4:intergenic:Lrrtm4-Gm26264 2:intron:Rps6ka5 4:intergenic:Kcnh8-Efhb 3:intron:Frmd5 4:intergenic:Ttc6-Sstr1 4:intron:Gnb1l 2:exon:Zmat1 4:intergenic:Gm23061-Ostf1 4:intergenic:B4galt5-Slc9a8 4:intron:Larp1 3:intron:Pak7 4:intergenic:Gm7985-Daam1 3:intergenic:Gm6938-Gm26593/Gm5124 2:intergenic:Gm5973-Gm9915 4:intergenic:Gm21833-Rpl21-ps5 4:intergenic:Dcaf12l2-Itpa-ps3 4:intergenic:Cmpk2-Gm9866 4:intergenic:Gm7951-Gm17064 4:intergenic:Gm6482-Gm7953 4:intergenic:Gm3141-Gm7233 4:intron:Oacyl 4:intron:Klhl14 4:intergenic:Gm12126-Tenm2 4:intergenic:Gm26022-Chl1 3:intergenic:Gm23494-Gm15679 4:intron:Cog5 4:intergenic:Gm9001-Gm22283 4:intron:Kcnc2 2:intron:Dok6 4:intron:Arhgef18 3:intron:Pcsk5 4:intergenic:Pabpc6-Qk 4:intergenic:Metap1d-Gm23253 4:exon:Wdr45b 4:intergenic:Gm26755-Ssfa2 4:intergenic:Gm26101-Plscr5 4:intergenic:Spin2d-Xlr3b 4:intron:Gm20455 4:intergenic:Gm25021-Met 4:intergenic:Gm22438-Gm22159 4:intergenic:Pola1-Gm14780 4:intron:Shank2 4:intergenic:B930078G14Rik-Fam155a 4:intron:Ppp2r2b 4:intergenic:Gfra2-Gm9192 4:intergenic:Gm23477-Gm10780 4:intergenic:Robo2-Gm26307 4:intergenic:Agtr1b-Gm7488 4:intergenic:Iah1-Iah1/Adam17 3:intergenic:Gm25658-Atp2b1 4:intergenic:Zfp516-Gm24720 4:intergenic:Gm11404-Gm11221 4:intron:Gm15866 4:intergenic:Vmn2r-ps122-Vmn2r103 4:intergenic:Vmn2r92-Vmn2r94 4:intron:Gpr124 4:intergenic:Nrk-Serpina7 4:intron:Pak3 3:intergenic:Tox3-Chd9 4:intergenic:Gm24963-Gm10742 4:intron:Pik3ca 4:intron:Mvb12b 4:intergenic:Gm24921-Fscb 4:intergenic:mmu-mir-6335-B3galt1 4:intron:Arhgap15 3:intergenic:Prg4-Hmcn1 3:intergenic:Gm25598-BC002059 4:intergenic:Gm15566-Myrip 4:intron:Grm3 4:intergenic:Olfr1504-Olfr1505 4:intron:Tiparp 4:intergenic:Gm22536-Gm6632 4:intergenic:Gm25658-Atp2b1 4:intergenic:Gm23460-Nab1 4:intergenic:Gm9833-Pmp2 4:intergenic:Tmem132c-Slc15a4 4:intron:Kcnq5 4:intron:Grid2 4:intergenic:Sorl1-Sc5d 4:intergenic:Olfr1504-Olfr1505 4:intron:Hivep3 4:exon:Lars 4:intergenic:Dapp1-Gm5105 4:intron:Rbpms2 4:intergenic:5730522E02Rik-mmu-mir-6372 4:intergenic:Gm24957-Gm22492 Off-target primers show all... show less...
619 / rev	`TGGAAAGACTCCATTGTTGA GGG` `.................... ...` Enzymes: Bsp1286I, BaeGI Cloning / PCR primers	50	76	50	45	68	71	0 - 0 - 13 - 56 - 276 0 - 0 - 2 - 3 - 12 345 off-targets	4:intergenic:F630028O10Rik/Mir223-Vsig4 4:intron:Vps13b 2:intergenic:Smlr1-Tmem200a 4:intergenic:Gm12378-Gm12379 4:intergenic:Gm6625-Gm5356 3:intron:Rgs7 4:intergenic:Mafb-Gm14221 4:intergenic:Otud3-Rnf186 3:exon:Mtm1 4:intron:Slc22a29 3:intergenic:Snrpn/Snurf-Snrpn 4:intergenic:Vmn2r-ps5-Vmn2r4 4:intergenic:Gm13994-Slc24a5 4:intergenic:Adcy2-Gm26844 4:intergenic:Batf-n-R5s64 3:intergenic:Gm13497-Gm13499 4:intron:Nxpe3 4:intergenic:Gm8492-Gm17728 4:intergenic:Spred1-Mir674 4:intergenic:Prps2-Gm6744 3:intergenic:Olfr827-Neurod4 4:intergenic:Gm8018-Olfr290 3:intergenic:Gm12518-Gm26144 4:intergenic:Trim60-Trim61/Gm10664 4:intergenic:Gm25693-Gm12129 3:intron:Zfp668 4:intergenic:5033403F01Rik-Gm23972 4:intergenic:Gm12636-Gm12632 4:intergenic:Mir1932-Rnf213 4:intergenic:4930441J16Rik-Lnp 4:exon:Dapk1 4:intron:Cnga2 3:intergenic:Gm26235-Gm25392 4:intron:Ostm1 4:intergenic:Wsb1-Gm9964 4:exon:Lctl 4:intergenic:Gm24070-Gm24994 4:intergenic:Gm7745-Lrfn5 4:intron:Serpine2 3:intergenic:Gap-CAAA01098150.1 4:intergenic:Spaca1-Gm25623 4:intron:Pde4b 4:intergenic:Grem2-Rgs7 4:intergenic:Gm12411-Gm12408 4:intergenic:5033403F01Rik-Gm23972 4:intergenic:Gm23131-Lphn2 4:intergenic:Got2-Gm22780 4:intergenic:Gm14005-Gm14006 4:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:Gm24591-Gm26241 4:intergenic:Gm25658-Atp2b1 4:intergenic:Gm12442-E130309F12Rik 4:intergenic:Gm9915-2610017I09Rik 4:intergenic:Erich2-Gad1 4:intergenic:Gm23979-Gm22845 4:intron:Esrrg 4:intergenic:Mrpl15-Lypla1 4:intergenic:Gm13481-Gm13489 4:intron:Dnah9 4:intergenic:Gm8212-Gm8220 4:intergenic:Rnf212-Tmed11 4:intergenic:Mir466f-4-Irx1 4:intergenic:Cnga2-Magea4 3:exon:Exosc10 4:intergenic:Gm25875-Gm23835 4:intron:Galntl6 4:intergenic:Gm23059-Gm7745 4:intergenic:Pemt-SMCR2_1 4:intron:Sytl5 4:intergenic:Gm22259-Ftmt 4:intergenic:Tmem233-C330018A13Rik 4:intergenic:Rala-Yae1d1 4:intergenic:Gm22649-Usp29 4:intron:Fam110b 4:intron:Zfp407 4:intron:Cntnap4 4:intergenic:Gm12295-Gm26128 4:intergenic:Sh3bp4-Agap1 3:intergenic:Mfhas1-Gm22216 4:intergenic:Gm6767-Cblb 4:intergenic:Zfa-ps-Slc35f1 4:intron:Ophn1 4:intron:AC168977.1 4:intergenic:Gm3076-Slc44a5 4:intron:Sp110 4:intergenic:AC132444.2-Csprs 4:intergenic:AC125149.3-Gap 4:intergenic:AC133103.7-AC133103.2 4:intergenic:AC133103.2-AC133103.5 3:intergenic:Adss-Gm16432 4:intergenic:Gm21726-Atp6ap1l 4:intron:Ephb1 4:intergenic:Gm26256-n-R5s61 4:intron:2810403D21Rik 4:intergenic:Gm24792-Epha5 4:intron:Cdkl4 4:intron:Pak7 2:intergenic:Gm7224-Vmn2r59 4:intergenic:mmu-let-7j-Gm6214 4:intergenic:Gm5123-Dusp21 4:intron:Fyn 4:intergenic:Rgs14-Slc34a1 4:intergenic:Gm25021-Met 4:intergenic:B4galt4-Gm15575 4:intergenic:AC121988.1-Gm25740 4:intergenic:Dnajb6-Il6 4:intergenic:Gm25130-Gm22970 4:intron:Nedd4l 4:intergenic:Gm7367-A230006K03Rik 4:intergenic:Gm22848-Rp1 4:intron:Sult2a7 4:intergenic:Gm17508-St14 4:intergenic:Enox1-Gm6994 4:intergenic:Pou6f2-Vps41 4:intergenic:Gm14293-Gm14294 4:intergenic:Gm14765-Tab3 4:intergenic:Ifit2-Ifit3 4:intergenic:Agtr1b-Gm7488 3:intron:Cacna1c 4:intron:Csgalnact1 4:intergenic:A230028O05Rik-Tprg 4:intergenic:Il3-Acsl6 3:exon:Mtr 4:intergenic:Gm26354-Gm7536 4:intron:Slc35f1 4:intergenic:Gm25015-Gm7481 4:intergenic:A830009L08Rik-Ssbp2 4:intergenic:Zfp536-Gm22302 4:intron:Cntnap5a 3:intergenic:Bbs10-Nap1l1 4:intergenic:EU599041-Zfp715 4:intergenic:Mboat1-Agtr1a 4:intergenic:C130060K24Rik-Tnip3 4:intergenic:Gm2824-9230109A22Rik 4:intergenic:Gm27000/Gm26958/Mir1906-2/MEG3_2-Gm5759 4:intergenic:Pvrl3-Gm6912 4:intergenic:Gm20775-Gm20777 4:intron:2310002F09Rik 4:intergenic:Gm22966-Gm15443 4:intergenic:Gm24973-1700003E24Rik 4:intron:Hcn3 2:intron:Apbb1ip 4:intergenic:Ubash3a-Rsph1 4:intergenic:Gm24440-Lpp 4:intergenic:Gm22522-Gm15168 4:intergenic:A330048O09Rik-Zfp169 4:intergenic:Tapt1-Ldb2 4:intergenic:Gm22011-Mthfr-ps1 4:intron:Cspp1 4:intergenic:Ndfip1-Spry4 4:intergenic:St18-Pcmtd1 4:intron:Dock3 4:intron:Phf17 4:intergenic:Gm23985-Gm4915 4:intergenic:Pcmtd2-Polr3k 4:intergenic:Gm24633-Gm25568 4:intergenic:Gm5634-Gm14509 4:exon:Snx25 4:intron:Nav2 4:intron:Ptprg 4:intergenic:Smg6-Gm12333/Smg6 4:intron:Sestd1 4:intergenic:Gm22674-Cdyl 4:intergenic:Gm9921-Gm24948 4:intergenic:Tmem74-Trhr 4:intergenic:Tra2b-Gm15776 4:intergenic:Gm26005-Epcam 4:intergenic:Tead4-Gm26951/Rpl18-ps2 4:intergenic:Umodl1-Abcg1 4:intergenic:Olfr921-Olfr922 4:intron:Dnmt3b 4:intron:Abi3bp 3:intergenic:Gm25051-Gm1818 4:intron:Plac1 4:intergenic:Gm26937-Lipi 4:intergenic:Arhgap6-Hccs 4:intergenic:5930422O12Rik-Tex15 4:intergenic:Gm5732-Scgb1b21 4:intron:Pdyn 4:intron:Kansl1l 4:intergenic:Clnk-Gm25505 4:intergenic:Gm12374-Gm12375 4:intergenic:Gbp4/Gbp8/Gbp9-Gbp10 4:intergenic:Gm4491-Gjb6 4:intergenic:Gm23376-Cd1d2 4:intergenic:Gm23987-Fam135b 4:intergenic:Vmn2r-ps94-Gm16566 4:intron:Kit 4:intergenic:Gm21392-Gm23727 4:intergenic:Gm16391-Gm17538 4:exon:Patl1 3:intron:Mtus2 4:intergenic:Gm22825-Zfhx3 3:intergenic:Clec14a-Gm17529 4:intergenic:Cntn5-Gm25365 4:intron:Syt6 4:intergenic:Gm8935-Gm2464 4:intron:Zbtb7c 2:intergenic:Xpo7-Dok2 4:intergenic:Gm22136-Gm22206 4:intergenic:Tsku-Gucy2d 4:intergenic:Irf8-Gm26878 4:intergenic:Edil3-Gm26120 4:intergenic:Aard-Slc30a8 4:intron:Tmlhe 4:intergenic:Gm7722-Gm14609 4:intergenic:Baz2b-Gm13574 4:intergenic:Gm13494-Gm13491 3:intergenic:Fam169b-Gm5334 4:intergenic:Gm24129-Tecrl 4:intergenic:Gm11780-Gm26436 4:intergenic:Gm23769-Gm23203 4:intergenic:Gm23180-Gm22977 4:intergenic:Gm25972-Gm13711 4:intron:Frem2 4:intron:Tmc1 4:intergenic:Gm22183-mmu-mir-378d 4:intergenic:Stox2-4930448N21Rik 2:intron:Man1c1 2:intergenic:Fcgr4-Fcgr3 4:intergenic:Dazl-Plcl2 3:intergenic:Pcsk1-Gm4149/Mir682 4:intergenic:U7-Gm10192 4:intergenic:Gm25658-Atp2b1 4:intergenic:Skint11-Trabd2b 3:intergenic:Gm14467-Gm17014 3:intron:Gm13490 4:intergenic:Gm10097-Syne1 4:intergenic:4930578C19Rik-Gm6923 4:intergenic:Gm26317-Gm23794 4:intron:Cpa6 2:intron:Lrba 4:intergenic:Gm8919-Gm8935 2:intergenic:Tpbg-Ube2cbp 3:intergenic:Gm25714-Gm24997 4:intergenic:Gm23434-Cntnap5b 3:intergenic:Vsig4-Hsf3 4:intergenic:Sowahc-P4ha1 4:intergenic:Ptger4-AC133283.1 4:intron:Pax6 4:intron:Grip2 3:intergenic:Gm13868-Trim44 3:intron:Cngb3 2:intergenic:Pof1b-Gm14922/Gm14923 4:intergenic:Tenm4-Gm15414 4:intergenic:Gm14336-Gm14337 4:intergenic:Slco6c1-D1Ertd622e 4:intron:Gm973 4:intergenic:Gm11868-Cnbd1 3:intron:Lrrc7 3:intergenic:Fgf13-Gm14887 2:intergenic:Ctnna3-Gm22829 4:exon:A430110L20Rik 4:intron:Slc36a2 3:intergenic:Gm23379-Zranb2 3:intergenic:Gm23752-Gm24815 3:intergenic:Actr1b-Zap70 3:intergenic:Gm11219-Pappa 3:intron:Sos2 3:intergenic:Grm1-Shprh 4:intergenic:Ercc6l-Gm9103 3:intergenic:Gm23987-Fam135b 3:intergenic:Gm22227-Zfp703 2:intergenic:Gm25509-n-R5-8s1 3:intergenic:Defb41/Gm15386-Gm24075 4:intergenic:Gm25339-Gm7275 3:intergenic:Gm25396-Slc25a46 3:intron:Txk 3:intergenic:Gm10006-Rgs6 3:intron:Gm16630 3:intergenic:RP23-448C3.1/Trpm3-Trpm3 3:intergenic:Gm5082-Adtrp 3:intron:Ascc3 4:intergenic:Gm7936-Gm24396 4:intergenic:Gm25998-Ralgps1 4:intergenic:Gm25684-Gm9966 3:intergenic:Gm25722-Gm10051 4:intergenic:Idh2-Sema4b 3:intergenic:Tspyl5-Mtdh 4:intergenic:Negr1-Gm22458 3:intron:Fhod3 3:intergenic:Gm16685-Gm24614 2:intergenic:C3ar1-Necap1 4:intron:Mctp2 4:intron:Ralgapa2 4:intron:Slc4a10 3:intron:Ndufs4 3:intergenic:Ins1-Xpnpep1 4:intron:Sccpdh 4:intergenic:n-R5s61-Gm15571 4:exon:Wscd2 3:intergenic:Tm4sf1-Tm4sf4 4:intron:Esrrb 3:intergenic:C8a-1700024P16Rik 4:intron:Zfp71-rs1 2:intergenic:Gm13794-Gm13796 3:intergenic:Gm24728-Lmo4 3:intergenic:Gm24790-Klf6 3:intergenic:Pam-Gm7952 3:intergenic:Gm22527-Rnf144a 4:intergenic:AI467606-Qprt 4:intergenic:Zbtb18-1700016C15Rik 4:intergenic:Slc35f1-Gm25664 4:intergenic:Olfr724-Olfr725 4:intergenic:Gm26430-Gm23895 4:intergenic:Pcnxl4-Dhrs7 4:intergenic:Gm5257-mmu-mir-6343 4:intron:Cntln 4:intron:Nfam1 4:intergenic:Gm10309-Prkce 4:intergenic:Snx16-Gm22795 4:intergenic:Gm24467-Gm13456 4:intergenic:Rpl38-ps2-Gm11077 4:intergenic:Mrgprb2-Mrgprb3 4:exon:Zfp518b 4:intergenic:Gm26275-Arsj 4:intron:Nrcam 4:intergenic:Gm21614-Gm23249 4:intron:Dlgap1 4:intron:Grik2 4:intergenic:Gm8183-Tmem135 4:intergenic:Gm8609-Gm22543 4:intron:Gm27013 4:intergenic:Samd8-Gm23502 4:intergenic:Gm23149-Gm14464 4:intergenic:Gm26197-Akap11 4:intron:Slc38a4 4:intron:Cflar 4:intron:Cers6 4:intergenic:Gm8116-Col12a1 4:intron:Adamtsl1 4:exon:Exosc2 4:intergenic:Grxcr2-Sh3rf2 4:intergenic:Gm11862-Gm23975 4:intron:Gm2716 4:intergenic:9130204L05Rik-Gm23697 4:intergenic:3830417A13Rik-Slitrk4 4:intergenic:Il1rl2-Il1rl1 4:intergenic:C230014O12Rik-Pbx3 4:intron:Agbl1 4:intergenic:Gm13421-Rxra 4:intergenic:Gm25258-Hao1 4:intergenic:Gm4868-Gm23151 4:intergenic:Igkv5-43-Igkv12-41 4:intergenic:Efhb-Rab5a Off-target primers show all... show less...
311 / rev	`ATGAAAATGGCAAGACTCTG TGG` `.................... ...` Enzymes: HinfI Cloning / PCR primers	48	71	60	37	73	89	0 - 1 - 6 - 37 - 292 0 - 1 - 1 - 3 - 3 336 off-targets	3:intron:Elp4 3:intergenic:Ghrhr-Adcyap1r1 4:intron:Otud3 2:intron:Gm12474 4:intron:Iqsec3 3:intron:Pex7 4:intergenic:B3galtl-Rxfp2 4:intergenic:4930469K13Rik-Gm12121 4:intergenic:Ccdc71l-Nampt 3:intron:Kcnh8 4:intron:Uqcc1 4:exon:Vmn1r44 4:intergenic:Gm7150-Ubqln2 4:intron:Ctnna3 3:intergenic:Gm11498-Gm22702 3:intergenic:Gm2308-Vmn2r60 4:intergenic:Gm12878-Gm12892 4:intergenic:Gm12893-Gm12895 4:intergenic:Zc3h12b-1700010D01Rik 4:intergenic:Gm6771-Ptchd4 4:intron:Cap2 4:intergenic:Cadm2-Gm24681 4:intergenic:Gm26354-Gm7536 4:intron:Trim71 4:intergenic:Rcan1-2410124H12Rik 4:intron:Pds5b 4:intergenic:Gm13501-Gm13503 4:intron:Dusp27 4:intron:Sh3pxd2a 4:intergenic:Las1l-Msn 4:intergenic:St6galnac5-Gm16232 4:intergenic:Gm14569-Pgrmc1 4:intergenic:Ceacam14-Gm5155 3:intergenic:Gm26235-Gm25392 4:intergenic:Gm26713-RP23-125F8.1 4:intergenic:Satb1-RP24-465O15.1 4:intergenic:1700123L14Rik-Gm22971 4:intergenic:Mtpap-9430020K01Rik 4:intergenic:Gm3712-Gm14806 2:intergenic:Gm8183-Tmem135 3:intergenic:Gm23795-Gm5342 4:intron:Rgs8 4:intergenic:Gm5899-Gm23884 4:intergenic:Ccnf-Abca17 3:intron:Siah3 4:intergenic:Sox2ot-mmu-mir-6378 4:intergenic:Gm3890-Rpl39l 4:intergenic:Cep112-Axin2 3:intergenic:Apoo-ps-Zswim6 4:intron:Fam136a 4:intron:Ncam2 4:intergenic:Nrxn1-Nrxn1/Gm10308 4:intergenic:Fam169b-Gm5334 3:intergenic:Gm23980-Gm22810 4:intron:Ctnna3 4:intergenic:Gm24728-Lmo4 4:intron:Mcidas 4:intergenic:Gm22939-Cntnap2 3:intergenic:Ak3l2-ps-8430437L04Rik 4:intron:Rims2 3:intergenic:Gm13517-Gm13615 4:intergenic:Pabpc6-Qk 4:intergenic:Clec14a-Gm17529 4:intergenic:Dok5-Gm14263 4:exon:Gm13001 4:intergenic:Gja1-Gm9795 4:intron:Adcy1 4:intergenic:Tmem232-Vapa 4:intron:Rab37 4:intergenic:Gm22011-Mthfr-ps1 4:intergenic:Ssbp3-Mrpl37 4:intergenic:1500009L16Rik-Nuak1 4:intergenic:Gm25344-Tmem232 4:intergenic:Gm25051-Gm1818 4:intron:1700057G04Rik 4:intergenic:Gm12010-Gm12011 4:intron:Robo1 4:intron:Grwd1 4:intergenic:Gm22183-mmu-mir-378d 4:intergenic:Nkx2-5-Gm17382/Gm20468 4:intergenic:C330013F16Rik-Trap1a 4:intergenic:Gm15712-Zbtb20 4:intergenic:Dhrs3-Vps13d 4:intergenic:Lrig2-Gm26091 4:intergenic:Ldoc1l-Prr5 4:intergenic:Dnah5-Mir3964 4:intergenic:Rph3a-Ptpn11 4:intron:Zwint 4:intergenic:Gm15206-Reps2 4:intergenic:Olfr1396-Olfr56 4:intron:Capn8 4:intergenic:Pisd-ps2-Scaf8 2:intergenic:Nkain2-Trdn 4:intron:Abcc12 4:intergenic:Gm24462-C230034O21Rik 4:intergenic:Gm22544-Gm22509 4:intergenic:Cox7c-Gm27025 4:intergenic:Rnf219-Gm22021 3:intron:Grik1 4:intron:Adamts12 4:intergenic:Mmp15-Gtl3 4:intergenic:Epha7-Gm11917 4:intergenic:Fut10-Gm23539 4:intron:Gm26905 3:intron:Syt9 4:intron:Slc8a1 4:intron:Ntrk2 3:intergenic:Gm22494-Chrna7 4:intergenic:Gm25798-Tcerg1l 3:intergenic:Gm23225-Gm24127 4:intergenic:Dnajc1-Gm13354 4:intron:Cnn3 3:intron:Ccdc63 4:intron:Wwp2 4:intron:Dock9 4:intron:Skap1 4:intron:Cdh13 4:intergenic:4930405O22Rik-Gm17228 4:intergenic:Smug1-Cbx5 4:intergenic:Anapc4-Gm23532 4:intron:Ano6 4:intron:Lmcd1 4:intergenic:Ndufa12-Gm4792 3:intergenic:Pla2g2e-Otud3 4:intergenic:Gm22866-Gm5620 4:intergenic:Zfp943-Zfp947 4:intergenic:Krtap6-3-Gm6358 4:intron:Prmt3 4:intron:Slx4ip 4:intergenic:Pik3ip1/Gm12592-Pik3ip1 4:intron:Ppp2r5e 4:exon:Agbl4 4:intergenic:Gm7846-Gm597 4:intergenic:Man1a2-Gm12485 4:intron:Fbxl2 4:intergenic:Wdr17-Gpm6a 4:intergenic:Hlf-Stxbp4 4:intron:Gpbp1l1 3:intron:Ppp1r12b 4:intron:Dmxl2 4:intergenic:Gm20448-Dtnb 4:intergenic:Apol7d-Gm5528 4:intergenic:Stpg2-mmu-let-7j 4:intergenic:Gm5444-Gm24790 4:exon:Abcc5 4:intergenic:AC140331.1-Dsp/Gm10129 4:intergenic:Dcc-Gm25509 4:intergenic:C130026L21Rik-Gm26897 4:intron:Gcfc2 3:intergenic:Palld-4930512H18Rik 4:intergenic:1700125G22Rik-Nceh1 4:intergenic:Gm4868-Gm23151 4:intergenic:4930447F04Rik-Gm23028 4:intergenic:Iyd-Plekhg1 4:intergenic:Olfr513-Olfr514 4:intergenic:Fzd7-Gm26813 4:intron:Ankrd12 4:intron:Pde4d 3:intron:Cdk15 4:intron:Gmds 4:intron:Ltbp1 4:intergenic:Gm25958-Gm16294 4:intron:Kcnh7 4:exon:Gm20721/Gnas 4:intergenic:Bdh1-Gm26053 4:intron:Tcea3 3:intergenic:Gm8325-P2ry1 4:intron:Nceh1 4:intergenic:Ube2e3-Gm14466 3:intergenic:Cryl1-Gm23593 4:intergenic:4930569F06Rik-Suds3 4:intron:Mn1 4:intron:Lmx1b 4:intron:Armc4 4:intergenic:Gm27023-Gm25800 4:intergenic:Abcb11-Abcb11/Gm13613 4:intron:Tmem14a 4:intergenic:Gm12139-Gm12140 4:intergenic:Gm25434-Rergl 4:intergenic:Insl5-Wdr78 4:intergenic:Gm15297-Hmgn2l6 4:intergenic:Chchd7-Gm11808 4:intergenic:Olfr16-Olfr1408 3:intergenic:Gm23732-Wdr60 4:intergenic:C2cd5-Etnk1 4:intergenic:Gm23729-Cdkal1 4:intergenic:Gm24334-Gsc 4:intergenic:Gm23752-Gm24815 4:intergenic:Igkv15-103-Igkv14-100 3:intergenic:Pdgfc-Gm22531 4:intron:Sdk1 4:intron:Rttn 4:intergenic:Gm12472-Gm25262/Gm12473 3:intron:Exoc6 4:intron:Rtel1 4:intron:Ppp1r3a 4:intergenic:3100003L05Rik-Gm21957 4:intron:Lrp1b 4:intergenic:Nck2-1500015O10Rik 3:intergenic:Plac8-Coq2 4:intergenic:Gpr111-Cd2ap 4:intergenic:Gm22053-Fhit 4:intergenic:Gm25727-F830104G03Rik 4:intergenic:Rapgef5-Cdca7l 4:intron:Negr1 4:intergenic:Mir129-1-Lep 4:intron:Scn5a 4:intron:Adk 4:exon:Pcca 4:intron:Clip2 4:intergenic:Gm6564-AC158942.1 4:intron:Cntnap5b 4:intron:Gpr158 4:intron:Rbfox1 4:intergenic:Gm22977-Zpld1 4:intron:Lrp1b 4:intergenic:Serpinb9c-Serpinb9d 4:intron:Lrrc7 4:intron:Clybl 4:intergenic:Dmd-n-R5s9 4:intergenic:Gm13810-B230118H07Rik 4:intron:Syndig1 4:intron:Runx2 4:intergenic:Ppp1r13b-2010107E04Rik 4:intron:Dlg2 4:intergenic:Lhcgr-Fshr 4:intergenic:Mir1931-Cdk17 4:intergenic:Sim1-Gm26257 4:intron:Dock2 1:intergenic:Gm5594-Vmn2r-ps59 2:intergenic:Gm5593-Vmn2r60 3:intergenic:Gm14754-4930595M18Rik 4:intergenic:Gm23558-Galc 4:intergenic:Las1l-Msn 4:intergenic:Gm26320-Megf9 4:intron:Slc4a7 4:intergenic:AC121988.1-Gm25740 4:intergenic:Gm12324-Gm12325 4:intron:Mbnl2 4:intron:Crx 4:intron:Ccdc67 4:intergenic:Gm23249-Gm9726/mmu-mir-8099-2 4:intergenic:Csl-Kitl 4:intergenic:Gm11351-Gm11352 4:intron:Pcdh15 4:intergenic:Gm23351-A530084C06Rik/Foxq1 4:intergenic:Cntnap2-Cul1 2:intergenic:Gm25985-Gm6404 4:intergenic:Sncaip-Snx2 4:intron:Mug1 4:intron:Phlpp2 3:intron:Npas3 4:intron:Abca4 4:intron:Myo18b 4:intergenic:Vash2-Mfsd7b 4:intron:Zfp395 4:exon:Myo1b 4:intergenic:Mtmr3-Gm11032 4:intergenic:Gm13678-Fsip2 4:intergenic:Ak3-Rcl1 4:intergenic:Drd4-Deaf1 4:intergenic:Clic6-Runx1 4:intergenic:Gm24925-Gm6767 4:intergenic:Hs6st3-Oxgr1 4:intergenic:Gm8210-Nck2 4:intergenic:mmu-mir-6540-Gap43 4:intergenic:Abcd3-Arhgap29 4:intron:Ttbk2/Cdan1 4:intron:Slc2a13 4:intergenic:Fam107a-Oit1 4:intergenic:Gm23752-Gm24815 4:intron:Mycbp2 4:intron:B230314M03Rik 4:intergenic:RP23-282G22.2-RP23-443P12.1 4:intergenic:Mut-Gm6771 4:intergenic:Mdfic-Tfec 4:intergenic:Vmn2r120-Gm21834 4:intron:Bbs9 4:intergenic:Gm23194-1700067G03Rik 3:intron:Ccdc6 4:intergenic:Pydc3-AI607873 2:intergenic:Gm10048-Kcnip4 4:intergenic:Cacnb2-Gm13268 4:intron:Jakmip1 4:intron:Farsa 4:intron:AW822252 4:intergenic:Etd-Gm14597 4:intron:Myl12b 4:intron:Arhgap15 3:intergenic:Ankrd16-Gm13310 4:intergenic:AI606473-Tyw3 4:intron:Foxn1 4:intergenic:Gm10295-Mctp2 4:intron:Marveld3 3:intergenic:Fgfr2-Gm5903 4:intergenic:Thap1-Gm22227 4:intron:Alk 4:intron:Limch1 4:exon:Dtd1 4:intergenic:Smad2-Skor2 3:intergenic:Gm23485-Rpl34-ps1 4:intergenic:Fshr-Gm10184 4:intergenic:Stc2-Bod1 4:intergenic:Fxr2-Sox15 4:intergenic:Slc35f4-Gm22116 4:intergenic:Gm9931-Gm22966 4:intergenic:Edem1-Gm20387 4:intron:Ccm2 4:intergenic:Gm5611-Chordc1 4:intergenic:Fbxo15-Gm25453 4:intergenic:Gm22473-Tox/Gm11802 4:intron:Dgkb 4:intergenic:Gm26101-Plscr5 4:intergenic:Gm24680-4921530L21Rik 4:intron:Gpm6b 4:intergenic:Olfr926-Olfr930 3:intergenic:Dyrk1b-Eid2 4:intergenic:Gm5530-mmu-mir-6344 4:intergenic:Gm25335-Magi2 4:intergenic:Parvg-1810041L15Rik 4:intron:Pcdh11x 4:intergenic:Rfx3-Glis3 4:intergenic:Ccdc149-Lgi2 4:intergenic:Asxl3-Nol4 3:intergenic:Pea15b-Gm22011 4:intergenic:Gm23429-Gm24304 4:intergenic:Gm12010-Gm12011 4:intergenic:Gatm-Spata5l1 4:intergenic:Gm10797-Zfp365 4:intergenic:Camk2d-Gm15551 4:intergenic:Gm15975-Gm23896 4:intron:Cars 3:intron:Tmem38b 4:intergenic:Gm14889-Gm14888 4:intergenic:Tmem45b-Barx2 4:intergenic:Fra10ac1-Lgi1 Off-target primers show all... show less...
41 / rev	`GTGGGAAGATTTTAAAGACA AGG` `.................... ...` Not with U6/U3 Cloning / PCR primers	47	66	58	38	66	80	0 - 0 - 9 - 58 - 362 0 - 0 - 1 - 10 - 19 429 off-targets	2:intron:Pms1 4:intron:Cdc14b 3:intron:Riok2 4:intergenic:Fam19a1-Fam19a4 3:intergenic:Tnp1-Pinc 3:intergenic:Tnp1-Pinc 4:intergenic:Prpf18-Gm13196 3:intergenic:Gm6462-Gm25586 4:intergenic:Gm15168-Gm5764 4:intergenic:n-R5s209-Gm7846 4:intergenic:Kifc3-Cngb1 4:intergenic:Gm13218-Gata3 4:intergenic:Gm9578-Gm22891 4:intergenic:Gm14519-Gm14518 3:intergenic:Gm8707-Sorl1 4:intergenic:Gm12129-Gm12130 4:intron:Meis2 3:intergenic:Lama2-Gm10145 4:intron:Pacsin2 4:intergenic:Fam84a-Gm9847 3:intergenic:Zfp521-Gm5160 4:intron:Elmod1 4:intergenic:n-R5s209-Gm7846 4:intron:Gpc6 4:intergenic:Lipc-Aqp9 4:intergenic:5033403F01Rik-Gm23972 4:intergenic:Gm5160-Gm6457 4:intron:Scn1b 3:intergenic:Gm14835-4933401B06Rik 4:intergenic:n-R5s209-Gm7846 4:intergenic:Gm13810-B230118H07Rik 4:intergenic:Gm13812-Gm13810 4:intergenic:Gm23028-Slitrk2 4:intergenic:Gm26252-Gm22838 4:intergenic:4930405D11Rik-Kif2b 4:intergenic:Gm24591-Gm26241 4:intergenic:Zfp516-Gm24720 4:intron:Aim2 4:intergenic:Gm7816-Gm2810 3:intron:Vps8 4:intergenic:Gm23059-Gm7745 4:intron:Slc39a11 4:intergenic:Gm12469-Gm25687 4:intron:Eif5b 4:intergenic:Ttll7-Gm23131 4:intron:Eif4g3 4:intron:Mib1 4:intergenic:Sema6d-4930517E11Rik 4:intron:Sspo 4:intergenic:Gm9001-Gm22283 4:intron:Memo1 4:intergenic:Gm26228-Gdf10 4:intron:Msi2 4:intergenic:Itgbl1-Gm25272 4:intergenic:C230029F24Rik-PCGEM1 4:intron:Socs6 4:intergenic:Gm8330-Hivep2 4:intron:Cd247 4:intron:Zfp609 4:intron:Tacc2 4:intergenic:D7Ertd443e-Dock1 4:intergenic:Gm23703-Mitf 4:intron:Erc1 2:intergenic:Prlhr-Cacul1 4:intergenic:Gm5432-Lpin1 4:intron:Clip4 4:intron:Nos1ap 4:exon:Rtn3 4:intergenic:Iyd-Plekhg1 4:intron:Cacna1b 3:intergenic:Gm6771-Ptchd4 4:intergenic:Rere-Gm13092 4:intergenic:5031414D18Rik-Lrrc63 3:intergenic:Dcaf5-Scarna3b 4:intergenic:Gm26307-Gm26937 4:intron:Gm14033 3:intergenic:Map10-Ntpcr 4:intergenic:RP23-302F9.3-RP23-394M5.2 4:intergenic:Gm10797-Zfp365 4:exon:Ryr1/Gm26604 3:intergenic:1700017N19Rik-Gm26278 4:intergenic:Ticam2/Tmed7-Ticam2 4:intergenic:Gbe1-Gm24968 3:intergenic:Grem2-Rgs7 4:intron:Cdh9 2:intergenic:Gm26307-Gm26937 3:intergenic:Msr1-Fgf20 4:intergenic:Dhx35-Gm25090 4:intergenic:n-R5s30-Rbfox1 4:intergenic:Ttll7-Gm23131 4:intergenic:Gm11417-Asic2 2:intron:Shroom2 4:intergenic:Gm24062-Gm10259 4:intron:Fam193a 4:intergenic:Sdr16c6-Gm11807 4:intergenic:Gm12213-mmu-mir-7671 4:intergenic:Gm5494-Gm20580 4:intergenic:Efcab1-Gm7731 4:intergenic:Moxd1-Gm15271 4:intergenic:Olfr391-ps-Olfr392 4:intron:Ttn 4:intergenic:Gm15359-Gm6483 3:intergenic:Gm24691-Grm8 4:intergenic:Gm26291-Gm17377 4:intron:Crebbp 4:intergenic:Gm23296-Akap6 4:intergenic:Alcam-Gm25723 4:intron:R3hdm1 4:intergenic:Gm21232-Gm27044 4:intergenic:Stk10-Fbxw11 4:intergenic:Pex2-Gm25352 3:intergenic:Gm23452-Gm23900 3:intergenic:Gm6189-Dock10 4:intergenic:Rhcg-Ticrr 4:intron:Itpr1 4:intergenic:Olfr650-ps1-Trim6 3:intergenic:Gm8840-Ikzf2 4:intron:Snd1 3:intergenic:Metrnl-Gm12589 4:intergenic:Gm9871-Cntn3 4:intergenic:Fam19a2-Gm23777 4:intergenic:Fam189a2-1700021P04Rik 4:intron:Fam178b 4:intergenic:Gm16203-Sgcz 3:intron:Bcl2l1 4:intergenic:Stc2-Bod1 4:exon:Ccdc65 3:intergenic:Gm13217-1700061F12Rik 4:intergenic:Gm13814-Gm13814/Gm13817 3:intergenic:Snx7-Gm26871 4:intergenic:Bod1-D630024D03Rik 4:intergenic:Gm25514-Gm13981 4:intron:Csmd3 4:intergenic:Gm6092-Gm14807 4:intergenic:Gm20916-Gm21823 4:intergenic:Gm20853-Gm20855 4:intergenic:Gm21065-Gm21871 4:intergenic:mmu-mir-6336-Lrp1b 4:intergenic:B3gntl1-Metrnl 4:intergenic:Gm7763-Gm5185 4:intron:Xrcc5 4:intergenic:Gm14539-Gm23628 4:intergenic:Col11a1-Gm23750 4:intergenic:Gm15394-Gm24547 4:intron:Simc1 4:intergenic:Gm23342-Myt1l 4:intergenic:Gm17076-4930445B16Rik 4:exon:Alpk3 4:intergenic:Lphn3-Mir1187 4:intron:Slc8a1 4:intron:Pdzd8 4:intergenic:5430401F13Rik-Gm16571 4:intron:Kcnab1 4:intergenic:Gm15508-Spata17 4:intergenic:Gm6634-Gm8604 4:intergenic:Gm11621-Gm11622 4:intergenic:Gm17192-Gm24164 4:intron:Prdm16 4:intron:Sytl4 4:intergenic:Cadm2-Gm24681 3:intron:Lamtor4 4:intergenic:Amd1-4930520K10Rik/Cdk19 3:intergenic:Gm14491-Gm14512 4:intron:Adrbk2 4:intergenic:Lmbr1-Gm23801 3:intergenic:Gm11680-BC006965 4:intergenic:Gm8730-Cdh5 3:intergenic:1700018M17Rik-Hsdl2 4:intergenic:Anapc15-ps-4732465J04Rik 4:intergenic:Fam50b-Prpf4b 4:intergenic:Skor2-Ier3ip1/Hdhd2 4:exon:Ola1 2:intron:Bcl7c 4:intergenic:Tmem42-Tgm4 4:intergenic:Nefl-Nefm 4:intergenic:n-R5s7-Gm14702 4:intron:A230071N21Rik 4:intron:4932414N04Rik 4:intron:Sptbn4 4:intron:Ttc39b 4:intron:Fbxo42 4:intron:Galnt7 3:intron:Ldlrad3 3:intron:Ralgapa1 4:intron:Fras1 4:intergenic:Pfn2-Gm24531 4:intron:Gpc5 4:intergenic:Gm15664-Tmtc2 4:intergenic:B430305J03Rik/Rap2b-Gm8349 4:intergenic:Trim35-Stmn4 4:intergenic:Gm15573-Jazf1 4:intron:Cntn3 3:intron:Npl 3:intergenic:Ldb2-4930431F12Rik 4:intron:Rnf144a 4:intron:Abhd6 4:intergenic:Gm26474-RP24-351J24.2 4:intergenic:Ralgps1-Zbtb34 4:intergenic:Mdfic-Tfec 4:intron:Sycp2l 4:intron:Mark4 4:intergenic:Gm26651-Diras2 3:intergenic:Gm14904-Gm24624 3:intron:Arpc5 4:intergenic:Parm1-Gm5558 4:intron:Ccdc155 4:intergenic:Cxcr4-Gm26686 4:intron:Jakmip3 3:intergenic:Gm23980-Gm22810 4:intron:6820408C15Rik 4:intron:Prkar2b 2:intergenic:Zfp516-Gm24720 4:intergenic:Mgat2-Dnaaf2 4:intergenic:Gm26651-Diras2 4:intergenic:Qrfpr-Anxa5 4:intergenic:Arap3-Pcdh1 4:intergenic:Gm8349-Arhgef26 4:intron:Stat6 4:intron:Fgd5 4:intergenic:Gm24795-Gm8609 3:intergenic:Gm8805-Crygf 4:intron:Akap6 4:intron:Hdac8 4:intergenic:Gm22593-Mettl4 4:intron:Manba 4:intron:Pdk3 4:intergenic:Gm16223-Cpeb2/Gm7854 4:intergenic:Ran-Gpr133 4:intergenic:Frmd4b-Gm25852 4:intergenic:Hmx1-Cpz 4:intergenic:Aftph-Gm12036 4:intron:Tgfbr3 4:intergenic:Cdh7-Cdh19 4:intergenic:Nfkbid-Aplp1 4:intergenic:Slc7a15-Pum2 4:intergenic:Olfr183-Olfr186 4:intron:Zfp809 4:intergenic:Gm11625-Brca1 4:intergenic:Gm24508-Gm25292 4:intron:Chd7 4:exon:Txn2 4:intron:Fgf13 4:intergenic:Gm9157-Ftx 4:intron:Trip4 4:intergenic:Gm26970-Foxk1 4:intergenic:Gm23488-Psap 4:intergenic:G6pd2-Gm22273 4:intergenic:Gm25038-D16Ertd472e 4:intron:Arhgap24 4:intergenic:Hmga2-Gm24298 4:intron:Oog4 4:intron:Clec4b1 4:intergenic:Banp-Gm22 4:intergenic:Ankrd9-Rcor1 4:exon:Gemin7 4:intergenic:Npl-Rgs8 3:intergenic:Gm9621-Gm24200 4:intergenic:A1bg-Gm24696 4:intron:Tank 4:intergenic:Gm23892-Gm25382 4:intergenic:Slc38a4-Amigo2 4:intergenic:Gm13454-Gm13451 4:intergenic:Clca2-Clca4 2:intergenic:Ifng-Dyrk2/4932442E05Rik 3:intergenic:Gm23986-Gm24929 4:intergenic:Gm23986-Gm24929 4:intron:Astn2 4:intergenic:Slc38a4-Amigo2 4:intergenic:Ccdc121-Dnah14 4:intergenic:Gm23481-Gm25650 4:intron:Syt1 3:intergenic:Zfyve19/Dnajc17-Zfyve19 4:intergenic:Gm22618-Gm22976 4:intergenic:Gm5449-Drd1a 4:intron:Cdh2 4:intergenic:Cpxm1-Gm14042 4:intergenic:Gm12075-Gm12077 4:intergenic:Gm15975-Gm23896 4:intergenic:Slitrk1-Gm25901 4:intron:C77370 4:intergenic:Gm14040-F830045P16Rik 4:intergenic:Gm6569/Mroh5-Gm7935 4:intergenic:Gm22492-Pdcd6ip 4:intergenic:Tgfbr3-Gm8145 4:intergenic:Gm7488-Tbl1xr1 4:exon:4933426D04Rik 4:intergenic:4933427E13Rik-5730522E02Rik 4:intron:Dlgap1 4:intron:Ppp4r1 4:intergenic:Olfr987-Olfr988 4:intergenic:Gm24066-Gm6395 4:intergenic:Rbm24-Cap2 4:intergenic:Bmp10-Arhgap25 4:intergenic:Gm7910-Khdrbs2 4:intergenic:Zfp503-1700112E06Rik 4:intergenic:Gm24275-Gm12291 4:intron:Samd12 4:intergenic:Txnrd1-Gm4799 4:intergenic:Kit-Gm25374 4:intergenic:Gm26021-Hilpda 4:intron:Svil 4:intergenic:Acaa1a-Xylb 4:intergenic:Gm26005-Epcam 4:intergenic:Frem2-Gm24851 4:intron:Usp32 4:intergenic:Nkx6-1-Cds1 4:intron:Il1rapl1 4:intergenic:Gm16579-Tmprss11e 4:intergenic:L3mbtl3-Gm8709 3:intergenic:Rbp1-Rbp2 4:intergenic:Gm24591-Gm26241 4:intergenic:Rph3a-Ptpn11 4:intergenic:Gc-Npffr2 4:intron:Kcnj5 4:intron:Cbx2 4:intergenic:Thap1-Gm22227 4:intergenic:Gm24773-Ncam2 4:intergenic:Gm25344-Tmem232 4:intergenic:Grin2b/Gm14329-Grin2b 4:intergenic:Katnbl1-Emc7 4:intergenic:Pcnx-n-R5s62 4:intergenic:Kifc3-Cngb1 4:intergenic:Tshz2/Gm26883-Gm26883 3:intergenic:Gm12371-Gm12372 4:intergenic:Gm2574-Gm24728 4:intron:Babam1 3:intergenic:Gm14106-A530006G24Rik 4:intron:Zfp592 4:intergenic:Gm23154-Gm12604 3:intergenic:Mis12-6330403K07Rik 4:intergenic:Gm22966-Gm15443 4:intergenic:Apbb1-Hpx 4:intron:Hdx 4:intergenic:Vmp1-Gm11478 3:intron:Gtdc1 4:intron:Ccser1 4:intron:Gm12239 4:intergenic:Tjp2-Fxn 4:intron:Fam76a 4:intron:Ep400 4:intron:Lamb1 4:intron:BC048546 4:intergenic:Fam180a-Mtpn 3:intron:Ifi202b 4:intergenic:Sptlc2-Alkbh1 4:intron:Cadm2 4:intergenic:Gm7931-Slit2 4:intergenic:Egfem1-Mecom 4:intergenic:Igsf11-Gm24795 4:intron:Ldlrad4 4:intergenic:Zfp407-Cndp1 4:intergenic:Gm25935-Gm22593 4:intergenic:Ppapdc3-Prrc2b 4:intergenic:Gm23185-Gm23848 4:intergenic:Gm25839-4930587A21Rik 4:intron:Stag2 4:intron:Phactr2 4:intron:Tcf4 3:intergenic:Med13l-n-R5s175 4:intergenic:Nipa1-Rps12l1 3:intron:Rims2 4:intergenic:Gm26927-Gm17618 4:intergenic:9530027J09Rik-Gm22528 4:intergenic:C130071C03Rik/Mir9-2-Gm17750 4:intergenic:Gm16565/Cd247-Gm23402 4:intron:Nrcam 4:intron:Pdlim1 3:intergenic:Fbxo15-Gm25453 2:intergenic:Gm5420-Gm6846 3:intergenic:E130006D01Rik-Miat/MIAT_exon5_3/MIAT_exon5_2/Gm26953/MIAT_exon5_1 4:intergenic:Gm14885-BC049702 4:intergenic:3100003L05Rik-Gm21957 4:intergenic:4930458D05Rik/Agpat9-Agpat9 4:intergenic:Gm15800-Trafd1 4:intron:Srrm3 2:intron:Frem3 4:intergenic:D3Ertd751e-Gm25714 4:intergenic:Gm23891-1700018B24Rik 4:intron:Tenm2 4:intergenic:Gm15985-Gm6116 4:intron:Lmod2 4:intergenic:Usp3-Car12 4:intron:Uvrag 3:intergenic:Gm24774-Pcdh17 4:intergenic:Gm12029-Spred2 4:intergenic:Gdf6-4930448K20Rik 4:intron:Csnk2a1 4:intron:Rad21 3:intergenic:Gm26134-Gm23434 4:intergenic:BC030500-Gm24847 4:exon:Itpr2 4:intron:Hecw2 4:intergenic:Gm23943-4930558J18Rik 4:intergenic:Trib1-Gm7083 4:intron:Nkain3 4:intergenic:Lpar1-Gm12578 3:intergenic:Gm25656-Cntn6 3:intron:Col4a6 3:intergenic:Snap25-Gm14053 4:intron:Nfasc 4:intron:Gm26793/Fgf15 4:intron:Fyb 4:intergenic:4921524J17Rik/1700051O22Rik-Gpt2 4:intergenic:Gm11986-U7 4:intergenic:Gm25714-Gm24997 4:intergenic:Gm22495-Dtna 3:intron:Pknox2 3:intron:Fhit 3:intergenic:Gm23509-Pcdh9 3:intergenic:Slc17a6-Gm7336 4:intron:Agap1 4:intron:C1ql1 4:intergenic:Igkv6-32-Igkv8-30 3:intergenic:Nhlrc2-Adrb1 4:intron:Gm831 4:intergenic:Gm9578-Gm22891 4:intron:Hspa12a 4:intron:Nlrp5-ps 4:intergenic:Klk1b27-Klk1b21 4:intron:Uggt2 4:intergenic:Gm24555-Pid1 4:intergenic:Gm13604-B3galt1 4:intergenic:Gm5161-Ephb1 4:intergenic:Gm24880-Gm10172 4:intron:Loxhd1 4:intergenic:Thnsl2-Fabp1 4:intergenic:Znrf4-Safb2 4:intron:Nlk 4:intergenic:Gm26022-Chl1 Off-target primers show all... show less...
512 / fw	`AGATGATTCTTTTATTTTAC TGG` `.................... ...` Not with U6/U3 Inefficient Low GC content Enzymes: BmuI, Bse1I Cloning / PCR primers	45	61	25	-4	77	76	0 - 0 - 7 - 66 - 380 0 - 0 - 0 - 9 - 11 453 off-targets	3:intron:Phldb2 4:intergenic:Trank1-Dclk3 4:intergenic:A830012C17Rik-Gm11780 3:intergenic:4931406P16Rik-Lsm14a 4:intergenic:n-R5s95-Fam155a 4:intron:Btbd3 4:intergenic:Sox11-Gm24326 3:exon:Usp7 4:intergenic:Capza2-St7 4:intergenic:Gm12638-Gm12637 3:intron:Syt10 3:intergenic:2010109A12Rik-Ccng2 4:intergenic:Gm24692-Sptssa 4:intergenic:Gm9312-Gm16372 4:intergenic:Gm14260-Gm9873 4:intergenic:Gm23777-Slc16a7 4:intron:A830018L16Rik 3:intergenic:Gm5161-Ephb1 3:exon:Lipf 4:intron:Tbcd 4:intergenic:Crh-4632415L05Rik 4:intergenic:Acvrl1-Acvr1b 4:intergenic:Gm22669-AC102758.1 3:intergenic:Vdac3-ps1-Pou6f2 4:intron:Fam114a1 3:intergenic:Gm22326-Sv2b 4:intron:Mcm3ap 4:intron:Ap3b1 4:intergenic:Gm26454-Gm7456 4:intron:Kif2a 4:intergenic:Stxbp3b-Scgb2a2 4:intron:Vmn2r95 4:intergenic:Gm23745-Gm24177 4:intergenic:Gm11674-Gm11679 3:intergenic:Trpm3-C330002G04Rik 4:intergenic:Vmn1r-ps49-Vmn1r69 4:intergenic:Lcorl/Ncapg-Lcorl 4:intron:Pde11a 4:intergenic:Gm14293-Gm14294 4:intergenic:Nup210-Hdac11 4:intron:Grm8 4:intergenic:Cat-Gm13996 4:intron:Nkain2 4:intergenic:Fam198b-Gm6706 4:intron:Skap2 4:intron:Bdp1 3:intergenic:Brinp3-Gm9931 4:intergenic:Gm24921-Fscb 4:intergenic:Ears2-Ears2/Ubfd1 4:intergenic:Rgs6-Gm22149 3:intergenic:Gm21614-Gm23249 4:intergenic:Gm22251-Zfp521 4:intergenic:Gm15170-Gm8662 3:intron:Lama2 3:intergenic:Gm14516-Cypt1 4:intron:Mbd5 4:intergenic:Gm15579-Tnfrsf26 4:intergenic:Gm7910-Khdrbs2 4:intergenic:Gm25169-Efnb2 4:intergenic:Lrrc16a-Gm11343 4:intergenic:Gm13810-B230118H07Rik 4:intergenic:Gm23059-Gm7745 4:intergenic:Galnt3-Gm13616 4:intron:Xkr4 3:intergenic:Olfr350-Olfr351 4:exon:Pkhd1 4:intergenic:Gm24946-Plch1 4:intergenic:Gm10166-Gm9211 4:intergenic:Mylip-Gm23387 3:intergenic:Rarb-Thrb 4:intergenic:Kcnh8-Efhb 4:intergenic:Gap-Vamp7 4:intergenic:Gm16428-Gm7916 4:exon:Pou4f2 4:intergenic:Sv2c-Gm25213 4:intergenic:4932415D10Rik-Gm4924 4:intron:Glra2 4:intron:Pcdh9 4:intergenic:Gm22016-Cdc73 4:intergenic:Gm25344-Tmem232 4:intergenic:B3galt5-Igsf5 4:intergenic:Gm5620-Zic1 4:intron:Slc24a2 4:exon:Lurap1/Pomgnt1 4:intergenic:Gm13851-9430029A11Rik 4:intergenic:Col1a2-Gm26696 4:intergenic:n-R5s42-Fam19a5 4:intergenic:Tbc1d1-Gm25306 4:intergenic:Gm26119-Fzd8 4:intergenic:Gm25519-Gm5548 4:intergenic:Gm13185-Fam107b 4:intergenic:Kctd8/Gm24368-Gm23932 4:intergenic:n-R5s168-Plekha5 4:intergenic:Gm22633-mmu-mir-6341 4:intergenic:Gm7191-Gm23494 4:intron:Nkain3 4:intergenic:9330154J02Rik-Tpbg 4:intron:Rai14 4:intergenic:C230004F18Rik-Cdr1 4:intergenic:Adra2a-Gpam 4:intergenic:Gabpa-App 4:intergenic:Gm14100-Bmp2 4:intron:Sntg1 3:intron:Cnksr2 4:intergenic:Ppp1r15b-Plekha6 4:intergenic:Gm22406-Spry2 4:intron:Gm7073 4:intron:Nell1 3:intergenic:Zfp300-Ssxa1 4:intron:Galnt13 4:intergenic:Plekhm1-Lrrc37a 4:intergenic:Commd4-Trcg1 4:intergenic:Gm24288-Gm17215 4:intergenic:Slc22a6-Chrm1 4:intergenic:Gm13681-Gm13683 4:intergenic:Ube2e2-Gm15916 4:intergenic:Gm25519-Gm5548 4:intergenic:Copa/Ncstn-Copa 4:intron:Fbxl7 4:intergenic:Pabpc2-Yipf5 4:intron:Akap1 4:intron:Daw1 4:intergenic:Gm9800-Fhit 3:intron:Nbeal1 4:intergenic:Gm22016-Cdc73 4:intergenic:Gm12827-Gm12826 3:intergenic:Cerkl-Neurod1/Gm26755 4:intergenic:Glt1d1-Tmem132d 4:intergenic:Zcchc5-Lpar4 4:intron:Clptm1 4:intergenic:Kcnt2-Gm4845 4:intron:Tgm6 3:intergenic:Rag2/B230118H07Rik-Rag1 4:intron:Smyd3 4:intergenic:Bmp4-Gm15221 4:intergenic:Gm22282-Dnajc5b 3:intergenic:Gm23437-Gm24818 4:intron:Srrm3 4:intergenic:Gm13982-Gm13985 4:intergenic:A1bg-Gm24696 4:intergenic:Cry1-AC100386.1 4:intergenic:Usp32-Rpl13-ps1 4:intergenic:Galnt9-Noc4l 4:intergenic:Gm13943-Gm13649 4:intergenic:Pax2-Fam178a 4:intron:Egfem1 4:intergenic:Impg2-Abi3bp 4:intergenic:Gm4409/Lrrtm4-Lrrtm4 4:intergenic:Gm24508-Gm25292 4:intron:Wasl 4:intron:Exoc6b 4:intergenic:Gm8098-4930505A04Rik 4:intergenic:Eya4-Rps12 4:intron:Syt1 4:intergenic:Plekhb2-Gm25096 4:intergenic:Gm25659-Gm25644 4:intergenic:Wac-Gm5819 3:intron:Mboat2 4:intron:Ccser1 4:intergenic:Cep164-Gm7286 4:intron:Prom1 4:intergenic:Gm11221-Gm11222 4:intron:4930473D10Rik 4:intergenic:Gm22254-Gm8993 4:intron:Grik1 4:intergenic:Gm26177-Gm25602 4:intergenic:Gm24200-1190002N15Rik 4:intron:Tbc1d5 4:intergenic:Fam19a2-Gm23777 4:intergenic:Gm24132-Gm25160 4:intergenic:Tars-Npr3 4:intergenic:Gm24968-Gm22797 4:intergenic:Urb1-Eva1c 4:intergenic:Rnf217-Gm7909 4:intron:Agbl4 4:intron:Tln2 3:intergenic:Cstf2t-Prkg1 4:intergenic:4930564K09Rik-Fgg 4:intergenic:Gm5839-Gm25440 4:intergenic:Pabpc4l-mmu-mir-6379 4:intron:Galntl6 4:intergenic:Gm13471-Gm13481 4:intergenic:Cetn3-Gm24295 4:intron:Rev3l 3:intergenic:Gm23515-Gm15355 4:intron:Pdlim5 4:intergenic:Lpar4-P2ry10 4:intergenic:1190003K10Rik-Gm4810 4:intergenic:Gm25485-Gm12460 4:intergenic:G630016G05Rik-Gm13991 4:intergenic:Gm9008-Ctnna2 4:intergenic:Phox2b-Tmem33 4:intergenic:Cmtm8-Gpd1l 4:exon:Plekhg3 3:intergenic:Mtus1-Fgl1 4:intron:Cbfa2t2 4:intergenic:Gm24987-Socs6 4:intron:Ppp2r2b 4:intron:Atrip 4:intergenic:Lcmt1-Aqp8 3:intergenic:Olfr1228-Olfr1229 4:intergenic:Gm25670-Gm26255 4:intergenic:Gm14977-Gm4994 4:intergenic:Gm8730-Cdh5 4:intergenic:Ube2ql1-Med10/Gm26819 4:intergenic:Gm22418-AC167724.1 2:exon:Rab8a 4:intergenic:Gm24508-Gm25292 2:intergenic:Vmn1r212-Gm11320 4:intron:Bai3 4:intron:Auts2 3:intergenic:Gm3191-Gm17031 4:intergenic:Rlim-Gm14892 4:intron:Zfpm2 4:intergenic:Prl2c3-Prl2c2 4:exon:Gm14976 3:intergenic:Gm13470-Gm25264 4:intron:Srpx 4:intergenic:Gm8807-Dym 4:intergenic:Zdhhc21-Cer1 4:intergenic:Gm21833-Rpl21-ps5 4:intergenic:Gm8707-Sorl1 4:intergenic:Pdk4-Dync1i1 4:intron:Snx19 4:intergenic:Gm17604-Glra2 4:intron:Coro2b 4:intergenic:Tars-Npr3 4:intergenic:A530006G24Rik-Gm25516 4:intron:Sclt1 4:intergenic:Fam180a-Mtpn 4:intergenic:Krtap10-10-Gm10318 4:intron:Myo1d 4:intergenic:RP23-319C8.4-Gm3867 4:intergenic:Igf1-Pmch 4:intergenic:Gm11913-Epha7 4:intron:Jarid2 4:intergenic:Dennd5b-Gm22578 4:intergenic:Stpg2-mmu-let-7j 4:intron:Tesk2 4:intergenic:Cox7c-Gm27025 4:intergenic:Gm9387-Pitx2 4:intron:Erc2 4:intergenic:Pabpc6-Qk 2:intergenic:Gm5506-Gm5839 4:intergenic:Col1a2-Gm26696 4:intergenic:Hnf4a-Ttpal 3:intergenic:Gm9800-Fhit 4:intron:Cyp3a11 4:intron:Col4a5 4:intergenic:Gm5899-Gm23884 4:intergenic:Dpyd-Gm23432 4:intergenic:Gm11906-Gm11900 4:intergenic:Gm22308-Cbfa2t2-ps1 4:intron:Hip1 4:intergenic:Dock8-Kank1 4:intergenic:Hnf4g-Gm23237 3:intergenic:Gm24691-Grm8 4:intron:Bbs9 4:intron:Get4 3:intron:Sybu 4:intergenic:D3Bwg0562e-4833424O15Rik 4:intron:Rabgap1l 4:intron:Themis 4:intergenic:Gm6927-Gm14746 4:intergenic:Gm26177-Gm25602 4:intergenic:Gm24861-Chodl 4:exon:Serpine3 4:intergenic:Nckap5-Mgat5 4:intergenic:Gm15443-Gm23535 4:intergenic:Gm25547-Efcab1 4:intergenic:Gm24679-Gm22219 4:intergenic:Gm15241-Gpr64/Gm15241 4:intron:Wbscr17 4:intron:Cttnbp2 4:intergenic:Gm11562-Krtap4-1 4:intergenic:Gm26713-RP23-125F8.1 4:intron:Ccdc91 4:intron:Skint8 3:intergenic:Lrpap1-Adra2c 4:intron:C330013F16Rik 3:intergenic:Gm17215-DAOA-AS1_1 3:intergenic:Cep85l-Gm5423 4:intergenic:Ntm-Gm26435 4:exon:4931423N10Rik 4:intergenic:Rnf8-Cmtr1 4:intron:Mapre2 3:intron:Slc5a12 4:intergenic:Ctbp2-Tex36 4:intergenic:Gm5648-Gm14932 4:intergenic:Dnah7a-Stk17b 4:intergenic:Gm23036-Gm22034 4:intergenic:Csmd3-Gm23530 2:intron:Tmem117 4:intergenic:Gm17215-Gm26427 4:exon:Tmem55b 4:intergenic:Uchl5-Rgs2 4:intergenic:Gm12666-Gm12638 4:intron:Sema3a 4:intergenic:Nsmce2-Trib1 3:intergenic:Gm12918-Gm12919 4:intergenic:Drr1-Gm14520 4:intron:Gxylt2 4:intergenic:Gm24319-Psat1 4:intergenic:Gm22529-Gpr37 4:intron:Cd2ap 4:intergenic:Ola1-Sp9 4:intergenic:Olfr877-Olfr145 4:intron:Nrg2 4:intron:Rabl3 4:intron:Gm12648 4:intergenic:Ppp1r12a-Pawr 4:intron:Zfp407 4:intergenic:Tnfsf13b-Myo16 3:intergenic:Gm11801-Gm22473 4:intron:Acaca 4:intergenic:Llph-Hmga2/1700006J14Rik 3:intron:Dcc 4:intergenic:Gm23520-Gm12131 3:intergenic:Gm5152-Gm17091 4:intergenic:Gm25041-Gm25853 4:intergenic:Cck-Lyzl4 4:intergenic:Gm17072-Inpp4b 4:exon:Mbnl2 4:intergenic:Fbxl14-Wnt5b 4:intergenic:Gm12458-Gm25485 4:intron:4930432B10Rik 4:intron:Grid2 4:intergenic:Ttc32-Gm7099 3:intergenic:Gm15902-Stxbp6 4:intergenic:Zfp804a-A130030D18Rik 4:intron:Focad 4:intron:Btc 4:intron:Shisa9 4:intergenic:Gm25537-Gm24122 4:intron:Hsf1 4:intron:Arl15 4:intergenic:Gm4810-Cntnap3 4:intergenic:Gm8183-Tmem135 3:intron:Rapgef5 4:intron:Sema3a 4:intron:Greb1l 4:intergenic:Gm24555-Pid1 4:intergenic:Gm6912-Gm24651 4:intergenic:Lrrtm4-Gm26264 3:intergenic:Gm11911-Gm11907 3:intron:Kif27 4:intron:Myocd 4:intergenic:Gm15403-Gm24244 4:intergenic:Epha5-Gm24626 4:intron:Cntn5 4:intergenic:Ccdc71l-Nampt 4:intergenic:Gm10108-Sema3a 4:intergenic:Gja1-Gm9795 4:intergenic:Gm13912-Gm13916 4:intron:Phldb2 4:intergenic:Amn1-2810474O19Rik 4:intergenic:Gm8609-Gm22543 4:intron:4930509E16Rik 4:intergenic:Rims2-Gm25381 3:intergenic:Gm11882-Fbxl4 4:intron:Mid2 4:intergenic:Gm12680-Gm26069 4:intergenic:Ntf3-Kcna5 4:intergenic:Gm22949-3632451O06Rik 3:intergenic:Ins1-Xpnpep1 4:intergenic:Ccdc147-Sorcs3 4:intron:Angpt1 4:intergenic:Gm5529-Gm23934 4:intergenic:Rev1-Aff3 3:intergenic:Wdfy3-Arhgap24 4:intron:Itpr1 4:intron:Orc4 4:intergenic:Gm15405-Gm27039 3:exon:Olfr273 4:intergenic:Gm5152-Gm17091 3:intergenic:Gm24246-Pik3c3 2:intergenic:6030443J06Rik-Gm21846 4:intron:Gm26907 4:intergenic:Gm4822-Tpm3-rs7 4:intron:Cic 4:intergenic:Ctps2-Gm23501 4:intergenic:Gm17333-Gm25025 2:intron:Iqgap2 4:intergenic:Cacna1e-Gm9530 4:intergenic:Gzmk-BC067074 4:intergenic:4930415L06Rik-Gm6027 4:intron:Gm15632 4:intergenic:Ak3l2-ps-8430437L04Rik 2:intergenic:Sema3d-Gm23243 3:exon:Cep192 4:intron:Osbpl5 4:intergenic:Rfx5/B230398E01Rik-Pi4kb 3:intron:Sephs1 4:intergenic:Gm14013-Dcdc5 4:intergenic:Gm22802-Slitrk5 4:intergenic:Gm8508-Gm26779 4:intergenic:5430403G16Rik-4930522L14Rik 4:intron:Dmd 3:intergenic:Gm22127-Tenm2 4:intergenic:Gm22628-5033411D12Rik 4:intergenic:Gm22434-Prrx1 3:intron:Mapkbp1 3:intron:Etfa 3:intergenic:Zfp622-March11 4:intergenic:Gm13481-Gm13489 4:intergenic:Gm7180-Pde8a 4:intron:Zfp521 3:intergenic:Ofcc1-Gm9979 4:intergenic:Gm14519-Gm14518 4:intergenic:RP24-399L6.2-9230113P08Rik 4:intron:Unc5d 4:intergenic:Gm19840-Cdh18 4:intergenic:Peli1-Gm22600 3:intergenic:Gm7790-Gm14977 4:intergenic:Gm11260-Gm11261 3:intergenic:Gm22475-Tmem242 3:intergenic:Krtap8-1-Krtap7-1 4:intergenic:Gm6085-Gm25493 4:intergenic:Gm24731-Gm22076 4:intergenic:Plcg2-Sdr42e1 4:intergenic:Gm22251-Zfp521 4:intergenic:Gucy2e-Gm23194 4:intergenic:Csmd1-Gm25665 3:intron:Pclo 4:intergenic:Efcab8-Gm14494 3:intergenic:Gm13468-Gm13481 4:intergenic:A430092G05Rik-Arhgap15 4:intergenic:Mki67-Gm9341 3:intron:Adam12 4:intron:Slc25a20 3:exon:Cep110 3:intron:Vps13a 4:intergenic:Adck3-Psen2 3:exon:Hrasls 4:intergenic:Olfr827-Neurod4 4:exon:Socs6 4:intergenic:Impg2-Abi3bp 4:intergenic:1110059M19Rik-Gm14606 4:intergenic:Gm22227-Zfp703 4:intron:Fam149b 4:intergenic:Gm26651-Diras2 4:intergenic:Sphkap-Gm25754 4:intergenic:Gm26535-Gm24710 4:intergenic:Apol7a-Apol9a 4:intergenic:Ube2ql1-Med10/Gm26819 4:intergenic:Aff2-1700020N15Rik 4:intron:Lrp4 4:intron:4930563I02Rik 4:intergenic:1700122O11Rik-1700067P10Rik 4:intergenic:Gm22308-Cbfa2t2-ps1 4:intergenic:Gm26288-Atp10a 4:intergenic:Cntn5-Gm25365 3:intergenic:Gm7485-Gm14926 Off-target primers show all... show less...
125 / fw	`TGATATCTAACCCACATTTA TGG` `.................... ...` Inefficient Enzymes: BstDEI, BstXI, SmiMI Cloning / PCR primers	40	83	17	24	74	76	0 - 1 - 0 - 22 - 134 0 - 1 - 0 - 1 - 7 157 off-targets	3:intergenic:Olfr1000-Olfr1001-ps1 3:intergenic:Gm5937-Tbl1x 4:intergenic:Gm14602-Dcaf12l1 1:intergenic:Gm5593-Vmn2r60 4:intergenic:Arsj-Gm17225 3:intron:Otogl 3:intron:1110019D14Rik 3:intergenic:Pcdh20-Gm25886 4:intergenic:4933440J02Rik-Krr1 4:intergenic:Poteg-Gm1698 4:intergenic:Gm23987-Fam135b 3:intergenic:Gm24256-Grik2 3:intergenic:Gm23239-Ntrk3 4:intergenic:Fgfr1op-Ccr6 4:intron:Camk1d 4:intergenic:Gm25437-Gm22684 4:intergenic:Igsf10-Gm5709 3:intergenic:Csf2ra-Gm21060 4:exon:Gm11544 4:exon:Gm6634 4:intergenic:2410088K16Rik-Sh3bp4 4:intergenic:Gm11924-4930556G01Rik 4:intergenic:Gm14849-Gm378 3:intron:Vmn2r39 3:intron:Gm3563 3:intron:Vmn2r43 3:intron:Vmn2r50 4:intron:Lmo7 4:intron:1700034E13Rik 4:intergenic:Rassf10-Gm23662 4:intron:Slc2a13 4:intergenic:Zfp945-Vmn2r-ps128 4:intron:Ptprd 3:intergenic:Vmn2r74-Vmn2r-ps79 4:intergenic:Spef2-Gm26350 4:intergenic:Gm22347-Gm23764 4:intergenic:Cpa6-Gm22963 3:intergenic:Gm7847-Gm23674 4:intergenic:Gm22032-Gm22895 4:intergenic:Gm8526-Edil3 4:intergenic:Gm20470/Katna1-Katna1 4:intergenic:Odf2l-Col24a1 4:intergenic:1810046K07Rik-Gm7293 4:intergenic:Gm25096-Gm25634 4:intron:Pou2f1 4:intron:Gm3972 4:intergenic:Vmn2r-ps37-Vmn2r34 4:intron:Gm3962 4:intron:Vmn2r29 4:intron:Gm3978 4:intergenic:Gm14643-Gm14575 4:intron:Vmn2r-ps45 4:intron:Vmn2r46 4:intergenic:Vmn2r-ps41-Vmn2r37 4:intergenic:Gm13461-Gm13463 4:intergenic:Thbs1-Fsip1 3:intergenic:4930506M07Rik-Vax1 4:intergenic:Epha3-Csnka2ip 4:intergenic:Gm16260-4930517O19Rik 4:intron:Faf1 4:intergenic:Gm23773-Gm5496 4:intergenic:Epha5-Gm24626 4:intergenic:Gm9795-Gm9956 4:intergenic:Tmem47-Gm14754 4:intergenic:4933428C19Rik-Gm12391 4:intergenic:4933433F19Rik-Dusp26 4:intergenic:Gm10097-Syne1 4:intergenic:Agrp-Fam65a 4:intergenic:Gm23520-Gm12131 4:intron:Tcf7l2 4:intergenic:Gm6634-Gm8604 4:intergenic:Oas1b-Oas1f 4:intergenic:Gm11676-Gm11677 3:intergenic:Cdh26-Gm14314 4:intergenic:Gm25813-Gm23666 4:intergenic:Gabrr2-Gabrr1 4:intergenic:D8Ertd82e-Lonrf1 4:intergenic:Gm26310-Grm8 3:intergenic:2010109A12Rik-Ccng2 4:intergenic:Sfi1/Drg1-Sfi1 4:intergenic:Rnf148-Cadps2 4:intergenic:Rnf148-Cadps2 4:intergenic:RP24-325L16.1-RP23-417D20.1 4:intergenic:Gm13975-Gm13974 4:intergenic:Gm25297-Dlk1 4:intron:App 4:intergenic:Gm11880-Gm11882 4:intergenic:Gm24792-Epha5 4:exon:Cenpf 4:intron:Igdcc3 4:intergenic:Wdr70-AC123611.1 4:intron:Rrp7a 4:intergenic:Gm14754-4930595M18Rik 4:exon:Rad51/Rmdn3 4:intergenic:Gm14494-Efcab8 4:intron:Cntn1 3:intergenic:Stk3-Osr2 4:intron:4930419G24Rik 4:intergenic:Gm26126-Gm11114 4:intron:Chsy3 4:intergenic:Gm14945-Gm4992 4:intergenic:Ptger4-AC133283.1 4:intron:Zmiz1 4:intergenic:1500009L16Rik-Nuak1 4:intergenic:Olfr1294-Olfr1295 4:intron:Lrch2 4:intron:Vwa8 4:intergenic:Tspan12-Ing3 3:intron:Sulf1 4:intergenic:Gm22883-Ppm1e 4:intergenic:Igsf1-n-R5s6 4:intergenic:Uaca-Gm10655 4:intergenic:Fbxo16-Zfp395 4:intergenic:Klhl25-Gm5597 4:intergenic:Gm11279-Gm11280 4:intergenic:Gm25378-Gm11908 4:intergenic:Abhd13-Tnfsf13b 4:intergenic:Tmed1-AB124611 4:intergenic:Gm8349-Arhgef26 4:intergenic:Gm14849-Gm378 4:intergenic:Gm5432-Lpin1 4:exon:Ccdc127/Lrrc14b 4:intron:Macrod2 4:intergenic:Nlgn1-A830092H15Rik 4:intergenic:Gm23503-Fign 4:intergenic:Csnka2ip-4930453N24Rik 4:intergenic:Gm5513-Gm24319 4:intron:Nell1 4:intron:Cnot2 3:intron:Utrn 4:intron:Esr1 4:intergenic:Gm8604-MALAT1 4:intergenic:Gm26367-Gm24345 3:intron:Ildr2 4:intron:Unc79 3:intergenic:Ccdc85a-2810471M01Rik 4:intron:Cdh6 4:intron:Nup133 4:intergenic:Gm25702-Gm5473 4:intergenic:Mapk1ip1l-Lgals3 4:intergenic:Nrsn1-Tpi-rs8 4:intron:Slc35a2 4:intergenic:Gm23021-8430419K02Rik 4:intergenic:Gm25307-Gm5549 4:intergenic:Copa-Pex19 4:intergenic:Gm12680-Gm26069 4:intergenic:Gm26963-Ypel5 4:exon:Tshz3 4:intron:Kcnj6 4:intergenic:Gm22789-Gm12003/Gm22801 4:intergenic:Zbtb20-Gm25873 4:intergenic:Gm25785-Gm4950 4:intron:Smarca1 4:intergenic:Gm26235-Gm25392 4:intergenic:Gm22536-Gm6632 4:intron:Hecw2 4:intergenic:Oas1g-Oas1a Off-target primers show all... show less...
40 / rev	`TGGGAAGATTTTAAAGACAA GGG` `.................... ...` Not with U6/U3 Cloning / PCR primers	38	55	59	59	65	77	0 - 1 - 10 - 79 - 495 0 - 1 - 5 - 8 - 18 585 off-targets	More than 500 off-targets, showing 17 with score >1.0 3:intergenic:Snx7-Gm26871 3:intergenic:Gm22603-Gria1 3:intron:Bcl2l1 4:intergenic:Gm15359-Gm6483 1:intron:Ntm 3:intron:Dnah10 3:intergenic:Gm8730-Cdh5 3:intron:Rims2 3:intron:Rsbn1l 2:intergenic:Rassf6-Cxcl5 2:intergenic:Fhod3-Tpgs2 4:intergenic:Tmem42-Tgm4 4:intergenic:Gm23181-Fam174a 3:intergenic:Galnt13-Gm24549 2:intergenic:Gm25656-Cntn6 2:intron:Bcl7c 2:intergenic:Gm14835-4933401B06Rik Off-target primers show all... show less...
513 / fw	`GATGATTCTTTTATTTTACT GGG` `.................... ...` Not with U6/U3 Low GC content Enzymes: BmuI, Bse1I Cloning / PCR primers	37	62	52	28	77	83	0 - 0 - 11 - 86 - 472 0 - 0 - 2 - 12 - 39 569 off-targets	More than 500 off-targets, showing 24 with score >1.0 2:intergenic:Chn2-Gm15527 4:intron:Tm9sf2 3:intergenic:Gm11354-Gm11355 2:intergenic:Gm2308-Vmn2r60 2:intergenic:Olfr1299-Olfr1300-ps1 3:intergenic:Gm8215-Gm5390 4:intergenic:Gm12010-Gm12011 3:intergenic:Gm11369-Uqcrfs1 3:intergenic:Dleu2-Gm26969 4:intron:Timp3 3:intron:Dcc 4:intergenic:Csmd3-Gm23530 3:intergenic:Rmnd5b-N4bp3 3:intron:Mcm3ap 4:intergenic:Plekhm3-Rpl31-ps24 4:intergenic:Gm22866-Gm5620 4:intergenic:Gm11779-Gm11782 3:intergenic:Mal2-Nov 2:intergenic:Gm7224-Vmn2r59 4:intergenic:Gm13479-Gm13478 4:intergenic:Nyap2-Gm5530 3:intergenic:Gm14550-Gm14570 3:intron:C030047K22Rik 2:intergenic:Gm26975-Gm22479 Off-target primers show all... show less...
59 / rev	`AATCATGGCAGAAAAGAAGT GGG` `.................... ...` Enzymes: MboII Cloning / PCR primers	33	68	68	39	62	75	1 - 1 - 4 - 70 - 369 1 - 0 - 0 - 3 - 9 445 off-targets	0:intergenic:Gm2308-Vmn2r60 3:intergenic:Gm24780-Ttc14 4:intron:Fam199x 4:intergenic:Mir690-Mb21d2 2:intron:Slc24a2 4:intergenic:Gm16340-Mndal 3:intergenic:Gm25834-Arhgef37 4:intergenic:Gm25602-Tbc1d32 3:intron:Mapk10 4:intergenic:Olfr172-Olfr173 3:intergenic:Gm12649-Gm22619 4:intergenic:Gm23794-Nhs 4:intron:Nell2 3:intron:Bach2 4:intron:Pard3b 4:intron:Acvr2a 4:intergenic:Maob-Ndp 4:intergenic:Gm15189-Rps6ka3 4:intron:Slc4a5 4:intergenic:Mageb16-ps1-Trpc5 4:intergenic:Gm12122-Slit3 3:intergenic:Gm23447-Gm23773 4:intergenic:Gm25295-Gm24546 4:intergenic:Gm24948-Nova1 4:intergenic:Gm25793-Gm8534 4:intergenic:6430571L13Rik-Cacna2d2 4:intergenic:Gm23937-Gm22944 4:intergenic:Gpr27-Prok2 4:intron:Atp2b3 3:intergenic:Gm23979-Gm22845 4:intergenic:4930594M17Rik-Gm26012 4:intron:Zfp3 4:intergenic:Gm24508-Gm25292 4:intergenic:Gm649-Gm14805 4:intergenic:Gm14972-Gm382 4:intergenic:Sipa1l2-Map10 4:intergenic:Cep55-Ffar4 4:intron:Dab1 3:intergenic:Gm23273-9330158H04Rik 4:intergenic:Qrfpr-Anxa5 4:intergenic:Rbbp6-Tnrc6a 4:intergenic:Pcdh17-Gm23926 4:exon:D2Wsu81e/Endog 4:intergenic:Gm26288-Atp10a 4:intergenic:Bambi-Gm23643 4:intron:Rab3c 4:intergenic:Gap-Gm7346 4:exon:Psmc2 4:intergenic:Gm23339-Gm25570 4:intron:Zfp423 3:intergenic:D3Bwg0562e-4833424O15Rik 4:intergenic:Eepd1-Gm26190 4:intergenic:Nlrp5-Vmn1r-ps84 4:intergenic:Gm10254-Sertm1 3:intergenic:Veph1-Shox2 4:intron:Gm9733 4:intergenic:Fam110b-Ubxn2b 4:intergenic:Iqch-Gm25995 4:intergenic:Cd2ap-Tnfrsf21 4:intron:Il1r1 4:intergenic:Rpl31-ps15-Gm26404 4:intergenic:Cacna2d1-Gm8984 4:intergenic:Mc4r-Gnal 4:intron:Grin3a 3:exon:Col6a5 4:intergenic:Gm13988-Sema6d 4:intergenic:Sema3d-Gm23243 4:intron:Dmxl1 4:intron:Tgfa 4:intergenic:Gm13833-Gm13835 3:intergenic:Gucy1a3-Map9 4:exon:Trim23 3:intron:Hecw1 4:intergenic:Edem1-Gm20387 4:intron:Ap2b1 4:intergenic:D3Ertd751e-Gm25714 4:intergenic:Gm5432-Lpin1 4:intergenic:Gpr12-Usp12 4:intergenic:Sphkap-Gm25754 4:intergenic:Cyp19a1-1700104A03Rik 4:intron:Nemf 3:intron:Uri1 4:intron:Mms22l 4:intron:Fam19a2 4:intergenic:E130006D01Rik-Miat/MIAT_exon5_3/MIAT_exon5_2/Gm26953/MIAT_exon5_1 4:intergenic:Anks1-Gm15597 4:intergenic:Gm15501-Gm5899 4:intron:Brwd3 4:intergenic:1700019G24Rik-Dync1i1 4:intergenic:Gm26474-RP24-351J24.2 4:intron:Nlrc5 4:intron:Snx7 4:intron:Tbx15 4:exon:Sqle 4:intron:Gatad2a 4:intergenic:Ncam2-Gm23355 4:intergenic:Fam126a-Klhl7 4:intergenic:Ncam2-Gm23355 4:exon:Bend3 4:intergenic:Jazf1-Gm15572 4:exon:Cstf3 4:intergenic:Adamts5-Gm25715 4:intergenic:RP24-86O15.2-Gm10698 4:intergenic:Gm26291-Gm17377 4:intergenic:Zhx2-Derl1 4:intergenic:Gm13982-Gm13985 4:intergenic:Gm15155-Gm23404 4:intron:Ston1 4:intergenic:mmu-mir-6354-Gm5265 4:intergenic:Gm7816-Gm2810 3:intergenic:Otogl-Ppp1r12a 4:intergenic:Slc25a21-Prps1l3/Mipol1 4:intergenic:Gm13809-Gm13808 4:intron:Slc26a7 4:intron:Cdk8 4:intron:Ano5 4:intergenic:Gm14530-Rpl36-ps10 4:intergenic:Flrt2-1700019M22Rik 4:intergenic:F830001A07Rik-Rora 4:intergenic:Gm24968-Gm22797 3:intron:Mcf2l 4:intergenic:Gm23177-Gm6100 4:intron:Gm13754 4:intron:Sntg1 3:intergenic:Gm5593-Vmn2r60 3:intergenic:Gm12114-Fgf18 4:intergenic:Syt1-Gm22097 4:intron:Arnt2 4:intergenic:Fsip2-Gm13680 4:intergenic:n-R5s42-Fam19a5 4:intergenic:Gm22939-Cntnap2 4:intron:Rims1 4:intron:Fggy 4:intergenic:Prkacb-Gm10288 4:intron:Sh3pxd2b 4:intron:Pde4d 4:intergenic:Gm24591-Gm26241 4:intergenic:Gm25281-Btbd11 4:intron:Adamts12 4:intergenic:Htr5b-Gm8217 4:intron:Rap1gds1 4:intergenic:Thsd7a-Gm15529 4:intergenic:AC165087.1-Gm6741 4:intergenic:1700091E21Rik-St8sia3 4:intergenic:Cdh2-Gm15485 4:intergenic:Gm1527-Egfem1 4:intron:Dnah11 4:intergenic:Gm24070-Gm24994 4:intron:Stk33 4:intron:Zfand3 4:intergenic:Edem1-Gm20387 4:intron:Lrp1b 4:intergenic:Gm25344-Tmem232 4:intergenic:Fto-Gm3235 4:intergenic:Atp6v0d2-4930480G23Rik 4:intergenic:Gm22382-Gm22169 4:intergenic:Gm5909-Gypa 4:intergenic:Robo1-Gm25699 4:exon:Nuak2 4:intergenic:AI429214-Gm25126 4:intron:Camk1g 4:intergenic:2410004B18Rik-Syde2 4:intron:Micalcl 4:intergenic:Gm26653-Gm26105 4:intron:Spock1 4:intron:Zhx3 4:intergenic:Slco3a1-Gm22326 4:intergenic:Rgs5-Rgs4 3:intergenic:Gm6447-Gm15062 4:intergenic:Gm25798-Tcerg1l 4:intergenic:Sbspon-4930444P10Rik 4:intergenic:Adra2a-Gpam 4:exon:Ckap5 4:intergenic:Mrvi1-Ctr9 4:intergenic:Gm21614-Gm23249 4:intergenic:Gm25136-Grin2b/Gm14329 4:intron:Zfp444 3:intergenic:Gm5641-Gm26678 4:intergenic:Rbm38/Gm14453-Ctcfl 4:intergenic:AC154801.1-BC040587 4:intergenic:Ccdc53-Dram1 4:intergenic:Ccdc132-Calcr 3:intron:Diap2 4:intergenic:Gm6166-Rpp25 4:intergenic:Gm14219-Gm11445 4:intron:Rasgrf2 4:intron:Dkk2 4:intergenic:4930509K18Rik-Gm23338 4:intergenic:Gm23980-Gm22810 4:intergenic:Hhipl2-Gm8214 4:intergenic:Gm25659-Gm25644 4:intergenic:Dagla-Syt7/Gm26859 3:intergenic:Gm16391-Gm17538 3:intron:Nkain3 4:intergenic:Gm23555-Gm25476 4:intergenic:Gm15359-Gm6483 4:intergenic:Llph-ps1-Pik3r3 4:intergenic:Gm25495-Gm23225 4:intergenic:Gm8993-Gm9001 4:exon:Kcnq2 4:exon:Rnf216 4:intergenic:Cmpk2-Gm9866 4:intergenic:Gm13782-Cep41 4:intergenic:Gm22181-Gm26994 4:intergenic:Gm26089-Fat1 4:intron:Lgr4 3:intergenic:Nkx2-9-Gm15524 4:intergenic:Ccdc25-Scara3 4:intron:7530420F21Rik 4:intron:Cntnap2 4:intergenic:Gm2373-5330416C01Rik 3:exon:2810468N07Rik 4:intergenic:Gm13810-B230118H07Rik 4:intergenic:Gm13812-Gm13810 4:intergenic:Plk2-Actbl2 3:intergenic:Gm11797-Fam110b 3:intergenic:Ksr1-Gm11200 4:intron:Lmx1a 4:intergenic:Arf5-Fscn3/Gcc1 3:intergenic:Tpd52-Zbtb10 4:intergenic:Ranbp6-Il33 4:intergenic:Gm11042-Kcnh5 4:intergenic:Gm8995-Gm1966 4:intergenic:RP24-196H4.1/Gm4070-Gm4070 4:intergenic:RP23-142E18.4-Gvin1 4:intergenic:Agbl1-Gm23239 4:exon:Stk4 4:intron:Nipbl 4:intron:Abca4 4:intergenic:Akr1c13-Akr1c19 4:intron:Mthfd1l 4:intergenic:Pard3b-4930448K12Rik/Pard3b 4:intergenic:Gm14060-Btbd3 4:intergenic:Gm26685-Dpt 4:intergenic:Olfr1338-Olfr1337 4:intergenic:Gm25336-Zfp648 4:intergenic:Sfmbt2-Gm13265 4:exon:Bptf 4:intergenic:Gm11873-Nkain3 4:intergenic:Gm24254-4933436C20Rik 3:intron:Gm15155 4:intergenic:Gm14516-Cypt1 4:intergenic:Gm13460-Lrp1b 4:intron:2310001H17Rik 3:intergenic:Gm14285-4930425F17Rik 4:intergenic:Ahr-Agr3 2:intergenic:Gm25714-Gm24997 4:intron:Esr1 4:intergenic:Gm13335-Etl4 4:intergenic:Gm24415-Alg10b/mmu-mir-7648 4:intron:Arfgef2 4:intergenic:Frem1-Gm24884 4:intergenic:E230019M04Rik-Gm15046 4:intron:Bre 4:intergenic:Nol10-Odc1 4:intergenic:Lrch2-Gm16400 4:intergenic:Gm24474-Frmd6 4:intergenic:Gm24937-9330185C12Rik 4:intergenic:Gm23674-Gm26106 4:intergenic:Gm23958-Il1rapl2 3:intergenic:Pah-Gm16176 4:intergenic:Gm2574-Gm24728 4:intergenic:Gm22538-Bche 4:intron:Hecw2 3:intergenic:Gja1-Gm9795 4:intron:Hs3st2 4:intergenic:Gpc5-Gpc6 4:intergenic:Ica1-Nxph1 4:intergenic:Wwox-Gm23677 4:intergenic:4930412D23Rik-Gm25061 3:intergenic:Gm7823-Gm14979 4:intron:Slc4a4 4:intron:Adipor2 4:intergenic:Nsmce2-Trib1 4:intergenic:Gm14219-Gm11445 4:intergenic:Gad1-ps-Csl 4:intergenic:Epc1-4921524L21Rik 4:intergenic:Slc22a6-Chrm1 4:intergenic:Cdh7-Cdh19 4:intron:Cdk19 3:intergenic:Redrum-Zfp608 4:intergenic:Vmn1r82-Vmn1r83 4:intergenic:Mrs2-Dcdc2a 4:intron:Cdh12 4:intergenic:Gm8840-Ikzf2 2:intron:Myo3a 4:intron:Gm26743 4:intergenic:Stk31-Npy 4:intergenic:AC133283.1-Gm23139 3:intergenic:Gm13550-Ccdc148 4:intergenic:Gm11255-Gm11259 4:exon:Ift52 4:intergenic:Pdzrn3-Gm9871 3:intron:Nxn 4:intergenic:Gpr116-Mep1a 4:intergenic:Gm21833-Rpl21-ps5 4:intergenic:Gm9578-Gm22891 4:exon:Jag1 4:intron:Cplx2 4:intron:Elf5 3:intron:Drosha 4:intron:Wac 4:intergenic:Kcnt2-Gm4845 4:intergenic:0610011F06Rik-Rab40c 4:intergenic:Gm13421-Rxra 4:intergenic:Gm25124-Gm12801 4:intergenic:Olfr721-ps1-Slc4a7 4:intergenic:Rab3gap2/Iars2-Iars2 4:intergenic:Paqr3-Gm5560 4:intergenic:Gm22689-Gm9742 4:intergenic:9130204L05Rik-Gm23697 4:intergenic:Timm8a2-A330035P11Rik 4:intergenic:Tdp2-D130043K22Rik 3:intron:Slc8a1 4:intron:Pard3 4:intron:Ano2 4:intergenic:Gm25769-Gm11487 3:intergenic:Gm7375-Gm10230 3:intergenic:Gm9440-RP23-29K3.1 3:intergenic:Gm1993-Gm2003 3:intergenic:Gm2030-Gm2005 3:intergenic:Gm7398-Gm10147 3:intron:Gm3677 3:intron:Gm7437 3:intergenic:Gm7386-Gm10058 3:intergenic:Gm7391-Gm4836 3:intergenic:Gm14527-Gm3657 3:intergenic:Gm5934-Gm4297 3:intergenic:Gm2012-Gm2030 3:intergenic:Gm2759-Gm2768 3:intergenic:Gm7408-Gm10096 3:intergenic:Gm7438-Gm10487 3:intergenic:Gm5935-Gm2759 3:intergenic:Gm6121-Gm7365 3:intergenic:Gm14819-Gm4764 3:intergenic:Gm5169-Gm1989 3:intergenic:Gm2066-Gm7421 3:intergenic:Gm21699-Gm2288 3:intergenic:Gm14819-Gm4764 3:intron:Gm2309 4:intergenic:Ripk4-Prdm15 3:intergenic:Gm21392-Gm23727 3:intergenic:Gm26350-Prlr 4:intergenic:Ssmem1-Cpa2 4:intergenic:Mir1899-Trpc6 4:intron:Tmem135 3:intergenic:Schip1-Gm10292 4:intergenic:Ceacam3-Psg29 4:intron:Ism1 4:intron:Wdr20a 3:intergenic:Gm24922-Cdhr3 4:intergenic:Gm23891-1700018B24Rik 3:intergenic:Gm11680-BC006965 4:intergenic:Gm24221-Sstr4 4:intergenic:Bcl11a-Gm12064 4:intergenic:Gm5496-Gm4518 4:intergenic:Gm24350-Gm13464 4:intergenic:Gm22848-Rp1 4:intergenic:Gm14903-Gm14906 2:intron:Bend6 4:intergenic:Ikzf2-Spag16 4:intergenic:Zic3-Gm14649 4:intergenic:Gm13463-Lrp1b 4:intron:Gm14033 4:intergenic:Slc22a23-Pxdc1 4:intergenic:Gm25419-Rad23b 4:intergenic:Lphn3-Mir1187 4:intergenic:Olfr894-Olfr143 4:intergenic:Gm23501-Gm22746 4:intron:Reep1 4:intron:2610206C17Rik 4:intron:Ptprr 4:intron:Ccdc138 4:intergenic:Vmn1r237-Vmn1r-ps150 4:intergenic:Gpr158-Gm13379 4:intergenic:Mir187-2700062C07Rik 4:intergenic:Cdh6-Gm9630 3:intergenic:Gm23083-Gm24773 4:intergenic:Irf2bp2-Tomm20 4:intergenic:St6galnac5-St6galnac3 4:intergenic:Irx1-Rpl9-ps4 3:intergenic:Gm13916-Gm13915 4:intergenic:Sod3-Ccdc149 1:intergenic:Gm5594-Vmn2r-ps59 3:intron:Pde1c 3:intergenic:Gm4954-Gm6286 4:intergenic:Fbxo8-Hand2 4:intron:Chst11 4:intergenic:Ube2e1-Gm22350 4:intergenic:Gm24369-n-R5s50 4:intergenic:Gabarapl2-Adat1 4:intergenic:Rgs6-Gm22149 4:intergenic:Serpinb9c-Serpinb9d 4:intron:Ctnna2 4:intron:1700007B14Rik 4:intergenic:Gm22053-Fhit 4:intergenic:Gfra2-Gm9192 4:intergenic:Gm5386-Gm14652 4:intergenic:Gm14918-Gm14913 4:intron:Klhl29 4:intergenic:Megf9-Gm11227 4:intergenic:Gm13805-Gm13804 4:intergenic:Igkv5-39-Igkv12-38 4:intergenic:Gm25041-Gm25853 4:intron:Mical3 4:intron:Tmem117 4:intergenic:Gm26108-Pou3f4 4:intergenic:Nell1-Ano5 4:intergenic:Gm11410-Gm11406 4:intergenic:Cyp4a29-ps-Cyp4a12a 4:intergenic:Mir1931-Cdk17 4:intergenic:Gm13452-Gm13453 4:intron:Gpatch2l 4:intergenic:Sdr16c6-Gm11807 4:intergenic:n-R5s209-Gm7846 4:intergenic:Gm22294-Fundc1 4:intron:Nlrp1c-ps 4:intergenic:Gm10145-Ptprk 4:intron:Grin2b 4:intergenic:Gm12210-Gm11187 4:intron:Tnfaip8 4:intergenic:Prkaa2-Ppap2b 4:intergenic:Gm17618-Gm26913 4:intergenic:Gm16210-Rev3l 4:intergenic:Gm25519-Gm5548 4:intergenic:Gm22507-Trhde 4:intergenic:Celf4-Gm25845 4:intron:Mapt 4:intergenic:Gm11258-Gm11253 4:intergenic:Jakmip2-Spink3 4:intergenic:Gm22223-Gm22715 4:intergenic:Galnt3-Gm13616 4:intergenic:Gm8793-Nkain2 4:intergenic:Gm23024-Gm24901 4:intergenic:Gm26341-Amd2 4:intergenic:Gm14082-Gm11644 4:intergenic:Gm24250-Nlgn1 4:intergenic:Gm12292-Elac2 4:intergenic:Vmn2r-ps120-Vmn2r99 4:intergenic:Olfr541-Cyp2e1 4:intron:Smad2 4:intergenic:Ano2-Ntf3 4:intergenic:Gm26171-Fam49a 4:intergenic:Gm7488-Tbl1xr1 4:intergenic:Gm17131-Tmem132d Off-target primers show all... show less...
359 / fw	`AATGCATGTTTTCTATTTTA TGG` `.................... ...` Not with U6/U3 Inefficient Low GC content Cloning / PCR primers	33	57	13	48	69	82	0 - 2 - 14 - 79 - 493 0 - 0 - 2 - 18 - 24 588 off-targets	More than 500 off-targets, showing 26 with score >1.0 3:intergenic:Gm13354-Gm13353 3:intergenic:Trp63-Gm24928 3:intergenic:Klhdc7a/Mir2139-Igsf21 4:intergenic:Gm23180-Gm22977 1:intergenic:Gm2308-Vmn2r60 1:intergenic:Gm7224-Vmn2r59 3:intergenic:Ptprm-Gm22277 3:intergenic:Gm14698-Gm6812 3:intron:2810032G03Rik 4:intron:Inpp4b 4:intron:Edil3 4:intergenic:Gm23225-Gm24127 3:intergenic:Gm25537-Gm24122 3:intron:Sh3bp4 3:intron:Kdelc2 4:intron:Vmn2r78 3:intergenic:Gm15399-Gm9372 4:intergenic:Gm6977-Gm44 3:intergenic:Cacna1d-Dcp1a 3:intergenic:Pld5-Gm26104 3:intergenic:Gm27023-Gm25800 3:intergenic:Olfr665-Olfr666 2:intergenic:Gm23757-L3mbtl3 2:intergenic:Gm24863-Gm24635 2:intergenic:Gm7763-Gm5185 2:intergenic:Fam19a2-Gm23777 Off-target primers show all... show less...
555 / fw	`CAGCACTATGAAACCTAGCC AGG` `.................... ...` Inefficient Enzymes: StyD4I, LpnPI, BstNI, MaeI Cloning / PCR primers	32	83	60	39	71	82	2 - 4 - 15 - 47 - 209 2 - 0 - 0 - 2 - 2 277 off-targets	0:intergenic:Gm8420-Stat4 4:intergenic:Gm16030-Gm16029/Egflam 2:intergenic:Gm5593-Vmn2r60 4:intergenic:4922501C03Rik-Tbx18 4:intron:Sgip1 4:intergenic:Ralgps1-Gm13537 4:intergenic:Gm2373-5330416C01Rik 4:intergenic:Gm13637-Gm13638 4:intron:Mrps35 1:intergenic:Gm14719-Olfr1320 3:intergenic:Pth-Far1 2:intergenic:Gm12295-Gm26128 4:intron:Hspg2 3:intergenic:n-R5s198-Gm13652 4:intergenic:Gm23752-Gm24815 3:intron:4931429I11Rik 4:intergenic:Taok1-Gm12732 3:intron:Gria1 3:intergenic:Cdh6-Gm9630 4:intron:Gab1 3:intergenic:Mir1952-Gm14065 4:intergenic:A930011G23Rik-Gm26252 4:intron:Fam53b 4:intron:Gm14717 4:intergenic:Ctps2-Gm23501 4:intergenic:Gm5670-1700112E06Rik 4:intergenic:Gorab-Mettl11b 3:intron:Als2 4:intergenic:Gm15529-Gm5300 3:intergenic:Gm7224-Vmn2r59 3:intergenic:Npsr1-Gm10701 3:intergenic:DLG2-AS1_1-Gm24552 4:intron:Bpifc 4:intergenic:Fam19a2-Gm23777 4:intron:Ltbr 4:intergenic:Gm5742-Gm22223 3:intergenic:Gm18367-Gm20713/Gpc5 4:intron:1700034G24Rik 3:intron:Slc13a1 3:intron:Tsc22d1 4:exon:4933411G11Rik 4:intergenic:Gm13754-Gm13749 4:intergenic:Gm25851-Gm14646 4:intergenic:Gngt1-Gng11 4:intergenic:Vmn2r97-Vmn2r-ps118 4:intron:Trpm8 3:intron:Plcb1 4:intron:Frmd4b 4:intergenic:Gm23971-Rnu2-10 4:intergenic:Rdm1-Gm24950 4:intron:Fam20c 3:intergenic:4933428C19Rik-Gm12391 4:exon:Crtc1 4:intergenic:Gm10660-Nat1 3:exon:Rsbn1/A130049A11Rik/Phtf1 4:exon:Prkar2a 3:intergenic:Gm26439-Rnft1 4:intergenic:Gm25722-Gm10051 4:intergenic:4933426K07Rik-Galnt10 4:exon:Atp11a 4:intergenic:Cnga2-Magea4 4:intron:Vrk1 4:exon:Zscan5b 4:intron:Zranb3 4:intergenic:Gm6741-Gm15404 4:intergenic:Nbas-Gm4929 4:intergenic:F2rl1-F2r 4:intergenic:Pou3f4-Cylc1 4:intergenic:Diras2-Syk 4:intron:Klhl7 1:intron:Acsm2 4:exon:Tgfa 4:intergenic:Gm12706-Ror1 4:intron:Ophn1 4:intergenic:Sumf1-Itpr1 4:exon:Rbm6 4:intergenic:Rgs13-Rgs1 4:intergenic:Gm14379-2010204K13Rik 3:intergenic:Il1rl2-Il1rl1 4:intron:Dcc 4:intergenic:Gm13787-1110051M20Rik 4:intergenic:Gm11804-8430436N08Rik 4:intergenic:Gm11214-Tnfsf15 4:intergenic:Ano6-D030018L15Rik 4:intron:Wdr70 2:intergenic:Med30-Ext1 4:intergenic:Galnt9-Noc4l 4:intron:Lpar1 4:intergenic:Gm17032-1700121N20Rik 0:intergenic:Crnn-Flg2 4:intergenic:Gm25335-Magi2 4:intron:Pias1 4:intergenic:Prkca-Gm11655 4:intergenic:Gm5973-Gm9915 4:intron:Lztr1 4:intron:Syn3 4:intron:Myl3 4:intron:Cnnm4 4:intergenic:Dennd1b-Crb1 2:intergenic:Las1l-Msn 3:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Gm25779-Gm13173 4:intergenic:Gm6706-Gria2 4:intergenic:Gpr141-Gm5446 4:intron:Nfkbie 4:intron:Slc25a26 4:intergenic:Dleu2-Gm26969 4:intron:9230110C19Rik 4:intron:Rap1gap2 4:intron:Atxn2 4:intergenic:Gm14606-Gm14605 4:intergenic:Gm16039-A430035B10Rik 3:intergenic:E130012A19Rik-Gm11613 4:exon:Car6 4:intergenic:Gm9387-Pitx2 4:intron:Dab1 2:intergenic:Gm25264-Gm25959 4:intergenic:Gm23514-5730522E02Rik 4:intergenic:Rap1gap-Alpl 4:intergenic:Gm24196-Adam22 2:intron:Ccdc14 4:intergenic:Tceb3-Gm24362 4:intergenic:Ttll7-Gm23131 4:intron:Fhad1 4:intergenic:Gm22273-Gm24079 4:intergenic:Gm10051-Gm15627 4:intron:Pcsk1 4:intron:Rnf32 4:intergenic:Gm5501-Thoc1 4:intron:Kcnh8 4:intron:Tbc1d10a 4:intergenic:Gm7091-Vsig1 4:intron:Casp14 4:intergenic:Olfr1507-Trav2 4:intergenic:Rbpms-Gm26978 4:intergenic:Phf15-Gm26551 4:intergenic:mmu-mir-6354-Gm5265 4:intergenic:PVT1_3-Ndufa4 3:intergenic:Gm16132-Fancc 4:intergenic:Gm24326-Gm25923 4:intergenic:Rbm27-Pou4f3 4:intron:1700046C09Rik 4:intron:Tnnt2 4:intergenic:Gm22183-mmu-mir-378d 4:intron:Nrxn3 4:intergenic:Gm22780-Gm24132 4:intron:Rora 4:intergenic:Gm5239-Snhg4 4:intergenic:Gm23755-Mylip 3:intron:AW209491 2:intergenic:Spef2-Gm26350 4:intergenic:4930586N03Rik-Zfp322a/4930586N03Rik 4:exon:Mib2 3:intron:Gm26691 3:intron:Itga9 4:exon:Dph3 4:intron:Dapk1 4:intergenic:Rbm24-Cap2 4:intron:Bcas3 3:intergenic:Gm2308-Vmn2r60 4:intergenic:Prkca-Gm11655 3:intergenic:Gmnc-Ostn 4:intergenic:Gm25550-Cdh9 4:intergenic:Zfp708-Rslcan18/Zfp708 4:intergenic:Gm5096-Gm24987 4:intergenic:Gtf3c5-Gm22675 4:intergenic:Spns3-Gm12712 4:intergenic:Gm25101-Cdh12 4:intergenic:4930483C13Rik-Grip1 4:intron:Dhx32 4:intergenic:Ugt1a7c/Ugt1a6a/Ugt1a8/Ugt1a10/Ugt1a6b/Ugt1a9/Ugt1a5/Ugt1a2/Ugt1a1/Gm20528-Mroh2a 4:intergenic:Ugt1a7c/Ugt1a6a/Ugt1a8/Ugt1a10/Ugt1a6b/Ugt1a9/Ugt1a5/Ugt1a2/Ugt1a1/Gm20528-Mroh2a 4:intron:Galnt18 4:exon:Vcam1 4:exon:Neu4 4:intergenic:Epha3-Gm22769 4:intergenic:Pcdh9-Gm23324 4:intergenic:4933434M16Rik-2610035D17Rik 4:intron:Dyrk1a 1:intergenic:Gm24948-Nova1 1:intergenic:Gm25094-Gm22312 2:intergenic:Slc39a8-Bank1 2:intergenic:n-R5s30-Rbfox1 2:intergenic:Impg1-RP24-325L16.1 2:intergenic:Fbxo15-Gm25453 2:intergenic:Gm14660-Sox3 2:intergenic:Lca5l-Sh3bgr 2:intergenic:Pros1-Epha3 2:intergenic:Gm25268-Ide 3:intergenic:Gm5342-Gm23815 3:intergenic:Gm23993-Adam22 3:intergenic:Gm17732-Spink13 3:intron:Plcl2 3:intergenic:Gm13849-1700012A03Rik 3:intergenic:PVT1_3-Ndufa4 3:intergenic:Bbs10-Nap1l1 3:intergenic:Gm25715-N6amt1 3:intron:Cthrc1 3:intron:Gm7073 3:intergenic:Osr1-Gm23979 3:intron:Eif2ak4 3:intergenic:Pcdh17-Gm23926 3:intergenic:Gm23033-Gm24554 3:intron:Snd1 3:intergenic:Gm379-Brwd3 3:intergenic:Gm7627-Gm24120 3:intergenic:Spp2-Glrp1 3:intron:Ccdc18 3:intergenic:Gm11919-Gm11923 3:intergenic:Tbc1d5-Satb1 4:intergenic:Stc2-Bod1 4:intergenic:Gm23771-AC132241.1 4:intergenic:Hspa1l-Gm10501/Hspa1l 4:intergenic:Gm23304-Gm24341 4:intergenic:Gm12911-Brinp1 4:intergenic:Tsku-Gucy2d 4:intergenic:Gm26819/Med10-Gm6132 4:intergenic:Gm22777-Spock1 4:intergenic:Gm11613-Gm11613/Gm24158 4:intron:Trip13 4:intergenic:Gm26915-Nrip1 4:intron:Arhgap20 4:intergenic:Pabpc2-Yipf5 4:intergenic:Rpl35a-ps2-Cnih3 4:intergenic:Gm25802-Gm12464 4:intron:Csmd1 4:intron:Rab27b 4:intergenic:Mcu-Micu1 4:intergenic:Gm11077-Pde3a 4:intron:Xkr4 4:intergenic:Rorb-Gm25437 4:intergenic:Gm6180-Gm23531 4:intergenic:Pole2-Klhdc1 4:intergenic:Gm22438-Gm22159 4:intergenic:Lphn3-Mir1187 4:intron:Camta1 4:intergenic:C1qtnf3-Amacr 4:intron:Tacr3 4:intron:Tmco3 4:intron:Kpna2 4:intergenic:Cts6-Cts8 4:intergenic:1110057P08Rik-Krtap20-2 4:intron:Tmem132c 4:intergenic:Gm4986-Gm14643 4:intergenic:Gm13913-Gm13914 4:intergenic:Vmn2r99-Vmn2r-ps121 4:intergenic:AI429214-Gm25126 4:intergenic:Gm6214-Pdha2 4:intergenic:5430427M07Rik-Cep128 4:intergenic:AC114591.1-Slco3a1 4:intergenic:Gm4950-Gm22259 4:intergenic:Gm5446-Elmo1 4:intergenic:Gm15496-Slc7a14 4:intergenic:Otogl-Ppp1r12a 4:intron:Osbpl9 4:intergenic:Arnt2-Gm2115 4:intergenic:Eda2r-Gm9062 4:intron:Bbs9 4:intergenic:Fam78b-Uck2 4:intergenic:Eif5al3-ps-Cox18 4:exon:Rab11b 4:intergenic:Gm12609-Gm12610 4:intergenic:Insl5-Wdr78 4:intron:Prkdc 4:intergenic:4930586N03Rik-Zfp322a/4930586N03Rik 4:intergenic:Krtap8-1-Krtap7-1 4:intron:Gm16223 4:intergenic:Gm25269-Ush2a 4:intergenic:Gm7976-Kcnj15 4:intergenic:Gm25336-Zfp648 4:intron:Cntn5 4:intergenic:Hcrtr2-Fam83b 4:intergenic:Slc9a1-Gm13257 4:intergenic:Gm6189-Dock10 4:intron:Zfp330 4:intergenic:Gm22713-Prss12 4:intergenic:Gm25813-Gm23666 Off-target primers show all... show less...
60 / rev	`CAATCATGGCAGAAAAGAAG TGG` `.................... ...` Enzymes: MboII Cloning / PCR primers	30	62	53	38	62	64	0 - 1 - 11 - 62 - 388 0 - 1 - 3 - 8 - 11 462 off-targets	1:intergenic:Gm2308-Vmn2r60 4:intergenic:Fto-Gm3235 4:intron:Gmcl1 4:intergenic:1700034B16Rik-Zbtb7c 4:intergenic:Gm22507-Trhde 4:intergenic:Ankrd34c-Mir184 3:intergenic:Gm11797-Fam110b 3:intergenic:Prrc2c-Fmo4 4:intergenic:Cstf2t-Prkg1 4:intergenic:4930511F01Rik-Snrpb2 4:intergenic:Chrm3-Gm26861 4:intergenic:Gm21614-Gm23249 4:exon:Dync1i2 3:intron:Fxr1 4:intron:Dzip3 4:intron:Gm13853 4:intron:Pde4d 4:intergenic:Cep55-Ffar4 4:intergenic:Gm23760-Gm23013 3:intergenic:1700125H03Rik-Csgalnact1 4:intergenic:Gm12178-Gm12179 4:intergenic:Gm12122-Slit3 4:intergenic:Gm23276-Dach1 4:intergenic:Gm24948-Nova1 4:intergenic:C430042M11Rik-Gm23905 4:intron:R3hcc1l 3:intergenic:Gm7485-Gm14926 4:intergenic:Gm22958-Fam19a2 3:intron:Dock10 4:intergenic:Gm25598-BC002059 4:intron:Fut9 4:intergenic:Gm23954-Pstpip2 3:intergenic:Ccdc53-Dram1 4:intergenic:Tnfsf4-Tnfsf18 2:intergenic:Grid1-4930596D02Rik 4:intergenic:Sec16b-Brinp2 4:intergenic:Gm24863-Gm24635 4:intergenic:Gm14060-Btbd3 4:intergenic:Arhgef11-Gm6570 4:intron:Pcmt1 4:intergenic:Gm5432-Lpin1 4:intergenic:Usp22-Rps13-ps5 4:intergenic:Gm22319-Adam7 4:intron:Zfp423 4:intergenic:Med13l-n-R5s175 4:intron:Hecw1 4:intergenic:Negr1-Gm15578 4:intergenic:6330419J24Rik-Gm14633/Gm2174 4:intergenic:Tns3-Gm11990 4:intergenic:Ncam2-Gm23355 4:intron:Lyg2 4:intron:Gemin8 4:intergenic:Gm13254-Gm24534 3:intergenic:AW209491-Gm6556 4:intergenic:Gm5507-Redrum 4:intergenic:Mterf-Akap9 4:intergenic:Sec11c-Grp 4:intergenic:Gm23937-Gm22944 4:exon:Otc 3:intergenic:Rpl31-ps15-Gm26404 4:intergenic:Gm23061-Ostf1 4:intron:Slc10a7 4:intergenic:Gm10800-Gm13809 4:intergenic:Cdh12-AC125320.1 4:intergenic:Gm13982-Gm13985 4:intergenic:Gm16340-Mndal 4:intron:Kdm5a 4:intergenic:Gm22538-Bche 4:intron:Ptprr 4:intergenic:A830092H15Rik-Gm25773 3:exon:Slc4a3 2:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Gm22283-Gm9008 4:intron:Setx 4:intergenic:Gm13466-Gm23072 4:intergenic:Agmo-Dgkb 3:intergenic:Zdhhc11-Gm15912 4:intergenic:Gm5874-Gm26809 4:intergenic:Timp2-Lgals3bp 3:intergenic:Rnf220-Gm12843/Rnf220 4:intron:Lpar1 4:intergenic:Agbl1-Gm23239 3:intergenic:Gm7823-Gm14979 4:intergenic:Pde4d-Rab3c 4:intergenic:Gm25567-Gm24385 4:intergenic:Gm5593-Vmn2r60 4:intergenic:Gm22118-Zswim6 3:intergenic:Lhfp-Nhlrc3/Gm16206 4:intergenic:Gm15251-Gm15257 4:intergenic:Gm14364-Gm14368 4:intergenic:Gm14358-Gm14362 4:intergenic:Gm21637-Gm21645 4:intergenic:Gm14359-Gm14355 4:intergenic:Gm15259-Gm15254 4:intergenic:Spin2f-Gm2784 4:intergenic:Gm5926-Gm21951 4:intergenic:Gm2964-Gm21870 4:intergenic:Gm14360-Gm14361 4:intergenic:Gm15275-Gm15274 4:intergenic:Gm2799-Gmcl1l 4:intergenic:Gm14360-Gm14361 4:intergenic:Gm21979-Gm21990 4:intergenic:Gm15281-Gm15279 4:intergenic:Gm4725-Gm9498 4:intergenic:Gm2825-Spin2-ps6 4:intron:Tmem37 4:intron:Acaca 4:intron:Gm15155 4:intron:Itih5 3:intron:Kcnj6 4:intergenic:Gm3160-Dlgap2 4:intergenic:Mdfic-Tfec 3:intron:Wdr70 4:intergenic:Gm9002-Kifc5c-ps 4:intron:D630045J12Rik 4:exon:Trbv17 4:intergenic:Gm23752-Gm24815 3:intron:Gpr141 4:intergenic:Lpp-A230028O05Rik 3:exon:Olfr398 4:intergenic:Gm24462-C230034O21Rik 4:intron:Faah 2:exon:Mroh2b 3:intron:Bend6 4:intergenic:Rdh14-Pgk1-rs7 3:intron:Nxf1 4:intergenic:Slc9a9-Chst2 4:intergenic:Cdkal1-Gm11364 3:intergenic:Runx1-AC124326.1 3:intergenic:n-R5s42-Fam19a5 4:exon:Kcnq2 3:intron:Fggy 4:intergenic:Ndufb5-Usp13 3:intergenic:Tex13-Gm7091 4:intron:Cbx7 4:intron:Dnah11 4:intron:Nrxn3 4:intergenic:Vmn1r82-Vmn1r83 3:intergenic:Gm25519-Gm5548 4:intergenic:Adra2a-Gpam 4:intergenic:Tdrd3-Rps3a2 4:exon:2810468N07Rik 4:intergenic:4933433F19Rik-Dusp26 4:intron:Odam 4:intron:Sgip1 4:intron:Ccser2 4:intron:Galnt12 3:intron:Skap2 4:intergenic:Gm23038-4930570G19Rik 4:intergenic:Gm26851-Tnmd 4:intergenic:Gm25987-Gm24834 4:intergenic:Gm23831-1700017G19Rik 4:intergenic:Gm11680-BC006965 4:intergenic:Samd5-Stxbp5 4:exon:Slc1a6 4:intergenic:Tcof1-Arsi 4:intergenic:Gm25798-Tcerg1l 3:intron:Mical3 4:intergenic:Ahr-Agr3 4:exon:Fhit 4:intergenic:Gm13685-Zc3h15 4:intergenic:Gm12035-Sertad2 4:intron:St8sia6 4:intergenic:Nps-4930544L04Rik 4:intergenic:Gm16205-Sgcz 3:intergenic:Gm11835-Gm11814 4:intergenic:Gm24340-Celf2 4:intergenic:Gm12034-Sertad2 4:intergenic:1700041M05Rik-Grik3 4:intergenic:Spidr-n-R5s31 4:intergenic:Gm9871-Cntn3 4:intergenic:Gm22495-Dtna 4:exon:Col6a5 4:intergenic:Gm22451-Sis 4:intron:Zfp444 3:intron:Rap1gds1 4:intergenic:Galnt13-Gm24549 4:intergenic:4930448E22Rik-Gm22659 4:intergenic:Gm5943-Gm14905 4:intron:A930006I01Rik 4:intron:Mbnl1 3:intergenic:Ipmk-Gm22937 4:intergenic:D3Ertd751e-Gm25714 4:intergenic:Gm23177-Gm6100 4:intergenic:Gm25650-Gm23584 3:intron:Bach2 2:intergenic:Gm23625-Mad2l1 4:exon:Jag1 4:intron:Gm13558 4:intergenic:Gm22448-Cadm2/1700010K23Rik 4:intergenic:Tmx1-Gm24474 4:intergenic:Olfr3-Olfr349-ps1 4:intergenic:Gm22828-Gm23960 4:intergenic:Anapc10-Hhip 4:intergenic:Gm21392-Gm23727 4:intergenic:Gm22024-Gm26721 4:intergenic:Mki67-Gm9341 4:intergenic:Olfr508-Olfr509 4:intergenic:Gm23903-Ank 3:intron:Galnt7 3:intergenic:D830044D21Rik-Gm26045 3:intergenic:Otud3-Rnf186 4:intergenic:Gm22382-Gm22169 4:intergenic:Qrfpr-Anxa5 4:intergenic:Disc1-Sipa1l2 4:intergenic:Gm22899-Rpl21-ps10 4:intergenic:Cry1-AC100386.1 4:intergenic:Gm94-Spink5 4:intergenic:Gm11352-1700092E19Rik 4:intergenic:Gm23239-Ntrk3 4:intron:Ccdc138 4:intergenic:Vmn1r27-Vmn1r-ps19 4:intergenic:Vmn1r19-Vmn1r20 4:intron:Dse 4:intergenic:Gm12008-Gm12009 4:intergenic:Gm25248-Gm26326 4:intergenic:Gm23331-Gm23708 4:intergenic:Gm22505-Gm12450 4:intron:Oxsr1 4:intron:Pld1 4:intergenic:Tex29-A230072I06Rik 4:intergenic:Gm12140-Gm12141 3:intergenic:Nr1h2-ps-RP24-376I11.1 3:intergenic:Abhd17b-Tmem2 4:intergenic:Gm11873-Nkain3 4:intergenic:Lmf1-Gm22645 4:intergenic:Gm10051-Gm15627 3:intergenic:Sh3gl2-Gm12680 4:intergenic:Odf2l-Col24a1 4:intergenic:Prrt1-Gm20461 4:exon:Grb10 4:intron:Dnah5 4:intergenic:4930564G21Rik-Grip1 4:intron:Morn3 4:intron:Cped1 4:intergenic:Gm5576-Reg3b 4:intron:Rnf165 4:intergenic:Gm23181-Fam174a 4:intergenic:Gm13755-Gm13756 4:intergenic:Gm25684-Gm9966 4:intergenic:Rab40b-Gm11866 3:intron:Tnfaip8 3:intergenic:Gm23447-Gm23773 4:intergenic:Fam19a2-Gm23777 4:intergenic:Gm20506-H2-Ab1 4:intergenic:Gm8840-Ikzf2 4:intron:Mtor 4:intergenic:Olfr1135-Olfr1136 4:intergenic:Gm23477-Gm10780 4:intergenic:Gm9885-Mrpl46 4:intergenic:4930428N03Rik-Gm10398 4:intron:Gm15479 4:intergenic:Gm22939-Cntnap2 4:intron:Pole4 4:intron:Rasgrf2 4:intron:Mical3 4:intron:Hs3st2 4:intron:Armc8 4:intergenic:Cphx2-Duxbl2 4:intergenic:Cphx1-Duxbl1 4:intergenic:Cphx3-Duxbl3 4:exon:Olfr715 4:exon:Gm10081 4:intron:Asph 3:intergenic:Gm12020-Gm12022 4:intergenic:Otoa-Hs3st2 4:intron:Drosha 4:intron:Nek1 4:intergenic:Mmd-Hlf 4:intron:Pard3 4:intergenic:Atp6v0d2-4930480G23Rik 4:intergenic:Gm13550-Ccdc148 4:intergenic:Gm23240-Gm8357 4:intron:Slc16a2 4:intergenic:Ccdc132-Calcr 4:intergenic:Pcgf5-Hectd2 4:intergenic:Gm24847-Gm26164 4:intergenic:9230109A22Rik-Basp1 4:intron:Fam212b 4:intergenic:Pou3f4-Cylc1 4:intergenic:Olfr612-Olfr613 4:intergenic:Nubpl-Arhgap5 4:intergenic:Gm26345-Gm10566 4:intergenic:Gm9439-Gm14558 4:intron:Fgf14 3:intron:Scoc 4:intergenic:Rspo3-Gm10275 4:intergenic:Unc5d-4933433F19Rik 3:intron:Slc39a11 4:intergenic:Rps18-ps2-Gm23423 4:intergenic:Gm23083-Gm24773 4:intron:Ptchd2 4:intergenic:Gm24285-4930467K11Rik 4:intergenic:Gm26861-Gm25496 4:intergenic:Gm24462-C230034O21Rik 4:intron:Cgnl1 4:exon:Pvt1 4:intergenic:Gm12210-Gm11187 4:intergenic:Mir466f-4-Irx1 2:intergenic:Gm22181-Gm26994 3:intron:Gprc5a 4:intron:Pcdh9 4:intron:Ppp2r2b 4:intron:Tmem132c 3:intergenic:Atrnl1-Gm16277 4:intergenic:Fam107b-Gm13180 4:intergenic:Gm25769-Gm11487 4:intergenic:Zfp808-Gm3604 3:intergenic:Tmtc4-Nalcn 4:intergenic:Gm14602-Dcaf12l1 2:intron:Adam20 2:intron:Adam39 2:intron:Adam25 4:intergenic:Sdr16c6-Gm11807 3:intergenic:Gja1-Gm9795 4:intergenic:Gm22848-Rp1 4:intergenic:Gm23979-Gm22845 4:intergenic:Ppm1a-4930447C04Rik 4:intergenic:Sdc1-9930038B18Rik 4:intergenic:Kif12-Col27a1 4:intergenic:Gm24070-Gm24994 4:intergenic:Ajap1-Gm25880 4:intergenic:Gm25261-Gm831 4:exon:A930011G23Rik 4:intergenic:Gm5697-Gm6462 4:intron:Pcsk2 4:intron:Atf6 4:intergenic:Rnf144b-4931429P17Rik 4:intergenic:Vps13c-Gm22382 4:intron:AW554918 4:intron:Ptdss2 4:intergenic:Gm20646-Mrps9 4:intergenic:Fam126a-Klhl7 4:intergenic:Gm26474-RP24-351J24.2 3:intergenic:Cadm2-Gm15828 4:intergenic:Gm22104-Gm25550 4:intron:Maml3 4:intron:Col26a1 4:intergenic:Six2-Srbd1 3:intron:Mcm3ap 4:intron:Lingo2 4:intergenic:Gm25258-Hao1 4:intron:Fam189a1 4:intergenic:B3galt1-Gm13603 4:intergenic:Ptplb-Adcy5 4:intergenic:Gm9578-Gm22891 4:intron:Vmn2r-ps56 4:intergenic:Gm94-Spink5 4:intron:Rev3l 4:intron:Osbp2 4:intergenic:Gm9884-Gm24064 4:intergenic:Igkv5-39-Igkv12-38 4:intergenic:Zic4-Gm26101 4:intron:Drosha 4:intergenic:Gm10247-Grin2a 4:intergenic:Gm23625-Mad2l1 4:intergenic:Sostdc1-Gm25438 4:intergenic:Rapgef5-Cdca7l 4:intergenic:Gm14055-Gm14064 4:exon:Trim37 4:intergenic:Gm17192-Gm24164 4:intron:Edil3 4:intergenic:Gm10275-Cenpw 2:intergenic:Gm25813-Gm23666 4:intron:Tmem132c 4:intergenic:Slc13a1-Iqub 4:intergenic:Gm13805-Gm13804 4:intergenic:Rarb-Thrb 4:intergenic:Gm4772-Gm4773 4:intergenic:Gm13780-Phf21a 4:intron:Wac 3:intergenic:Gm13659-Gm13658 3:intergenic:Prickle2-Adamts9 4:intergenic:Gm25552-Gm5382 4:intergenic:Gm8993-Gm9001 4:intergenic:4933406K04Rik-1700013N06Rik 4:intergenic:Gm24193-Gm20563 4:intergenic:Gm15425-Pde10a 4:intergenic:Npy-Gm21786 3:intron:Pard3 4:exon:Chst15 4:intergenic:1700055C04Rik-Gm25606 4:intergenic:Gm12114-Fgf18 4:intron:Cntnap4 3:intergenic:Gm26976-Cand1 4:intergenic:Gm23749-Gm13912 4:intergenic:Galnt1-Mir187 4:intron:Eif4g3 4:intergenic:Csgalnact2-Ret 4:intergenic:Gm15753-Nyap1 4:intron:Efhc1 4:intergenic:Gm22256-Zfp934 4:intron:Sh3tc2 4:intron:Kansl1 4:intergenic:Adam25-Adam20 4:intergenic:Adam24-Adam25 4:intergenic:Prkaa2-Ppap2b 4:intron:Prdm15 4:intron:Smim14 4:intergenic:Gm22069-Dscaml1 2:intergenic:9330111N05Rik-Gm8399 4:exon:Itgax 4:intron:Tmc1 3:intergenic:Fabp5l2-Mpp7 3:exon:Luc7l3 3:intron:Adam12 4:intergenic:Cts3-Gm19961 4:intergenic:Gm24588-Gm25448 4:intergenic:Gm24020-Gm5665 4:intergenic:Gm22256-Zfp934 4:intergenic:Ube2e1-Gm22350 4:exon:Ncoa7 4:intergenic:Gm23203-Gm23447 4:intergenic:Gm23838-Gm25233 4:intron:Fam19a2 3:intergenic:Gm25693-Gm12129 3:intron:Bai3 3:intergenic:Gm22546-Gm22194 4:intergenic:Gm9312-Gm16372 3:intergenic:Enpp3-Med23 4:intergenic:Tpd52-Zbtb10 4:intergenic:1700025G04Rik-Tsen15 3:intergenic:Gm12285-Gm12286 4:exon:Lctl 4:intergenic:Pah-Gm16176 4:intergenic:Arf5-Fscn3/Gcc1 4:intergenic:Foxo1/Gm20402-Foxo1 4:intergenic:Gm26455-Gm15754 4:intergenic:Epha4-Gm26263 3:intron:Sgsm3 4:intergenic:Rpl7l1-ps1-Gm14664 2:intron:Tprg 4:intergenic:Gm24326-Gm25923 4:intron:Lipc 4:intron:Cps1 4:intron:Lrrc48 4:intergenic:Agbl1-Gm23239 4:intergenic:Gm10051-Gm15627 4:intergenic:Gm23831-1700017G19Rik 4:intergenic:Lgmn-Golga5 3:intergenic:Dab1-Gm12719 4:intergenic:Gm807-Zfp366 4:intergenic:Gm4425-Gm20486 4:intergenic:Gm24734-Nog 4:intron:Fam111a 4:intergenic:Gm13800-Gm13801 4:intergenic:Gm5228-Gm14871 4:intergenic:Gm26350-Prlr 4:intron:Ppp1r32 4:intron:Gm20610 4:intergenic:Lgi2-Gm5866 4:intergenic:Gm11675-Gm11676 4:intron:Chst11 4:intergenic:Gm20472-Gm20473 4:intergenic:T-Pde10a 4:intergenic:Tpbg-Ube2cbp 4:intergenic:Fgf12-Mir690 4:intergenic:Gm13352-Commd3 4:intron:Kcnab1 4:intergenic:D3Ertd751e-Gm25714 4:intron:Iqcf3 4:intron:Opcml Off-target primers show all... show less...
758 / fw	`GAATTGTTATTCTAATTGTA TGG` `.................... ...` Low GC content Cloning / PCR primers	28	72	36	21	64	82	2 - 0 - 5 - 36 - 219 2 - 0 - 3 - 3 - 4 262 off-targets	0:intergenic:Gm5594-Vmn2r-ps59 0:intergenic:Gm2308-Vmn2r60 4:intron:Hdac9 4:intergenic:Gm13794-Gm13796 4:intergenic:4930448K12Rik-Pard3b 4:intergenic:Hdhd1a-Prr16 4:intergenic:Gm7729-Gm24246 3:intergenic:Atp6v1g3-Nek7 4:intergenic:Gm15210-Gm5742 3:intron:Scn5a 4:intergenic:Mir210-Phrf1 4:intron:Arhgap15 4:intergenic:Olfr228-Olfr1073-ps1 4:intron:Prkg1 3:intron:Tspan12 4:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:2210408F21Rik-Gm14532 4:intergenic:Gm9726/mmu-mir-8099-2-Gm26055 3:intergenic:Slc39a8-Bank1 4:intergenic:Vmn1r29-Gm20674 3:intergenic:Gm6863-Gm22230 4:intron:Gnaq 4:intergenic:Bche-Gm6098 4:intergenic:AC124193.1-Lsamp 4:intron:1110019D14Rik 4:intergenic:Vmn1r218-Vmn1r219 3:intron:Kif24 4:intron:Cdh6 4:intron:Klhl28 3:intergenic:Gm5593-Vmn2r60 4:intergenic:Gm25264-Gm25959 4:intergenic:Shroom4-Dgkk 4:intron:Glis1 4:intergenic:Gm5215-Colec10 4:intergenic:Gm25875-Gm23835 4:intron:Lrrk2 3:intron:Fggy 4:intergenic:Rassf9-Alx1 3:intron:Ar 4:intergenic:Gm4781-Tmtc3 4:intron:Dmd 4:intergenic:Gm13483-Gm13486 4:intergenic:Rasa1-Cox7c 4:intron:Tns1 4:intergenic:Gm24985-Vwde 3:intergenic:Gm22016-Cdc73 3:intergenic:Cnr1-Gm22010 4:intergenic:Gm26405-Gm9274 4:intergenic:Asb9-Gm15216 3:intergenic:Olfr383-ps1-Olfr385 4:intergenic:Smoc2-Thbs2 4:intergenic:Gm9396-Col25a1 4:intergenic:Gm20781-Rbm31y 4:intergenic:Gm21736-Gm21379 4:intergenic:Gm23059-Gm7745 4:intergenic:1700012B07Rik-1700023C21Rik 4:intergenic:Gm20713/Gpc5-Gm20713 4:intergenic:Lhcgr-Fshr 4:exon:Fkbp15 4:intergenic:Itga5-Gtsf1 3:intergenic:Gm25519-Gm5548 4:intergenic:4933433F19Rik-Dusp26 4:intergenic:Gm8604-MALAT1 4:intergenic:Gm25378-Gm11908 4:intergenic:Gm27037-Gm23859 4:intergenic:Gm7700-1110059M19Rik 3:intron:Rasgrp1 4:intergenic:Gm14373-Gm14452 4:intron:6530403H02Rik 4:intergenic:Gm3756-Arhgap32 4:intron:Syt16 4:intron:Gm6999 4:intergenic:Gm24232-Gm23987 4:intergenic:Gm17396-Rbms3 4:intron:Auts2 4:intergenic:Gm20516-Gm21947 4:intergenic:Dnah5-Mir3964 4:intergenic:Eda-Gm14832 4:intergenic:Gabrg2-A230004M16Rik 3:intron:Srpk1 4:intron:Npas3 4:intergenic:Gm14628-Gm14604 4:intron:Itih5 4:intergenic:Thumpd2-Slc8a1 3:intron:Gm26691 4:intergenic:Gm12010-Gm12011 4:intergenic:Gm11830-Trp53inp1 4:intron:Zdhhc21 4:intergenic:Gm25019-5530400C23Rik 4:intergenic:5530400C23Rik-Gm5885 4:intergenic:Gm22684-Anxa1 4:intergenic:Tas2r114-Gm4736 4:intergenic:Clic6-Runx1 4:intron:Scml2 3:intron:Khdrbs1 4:intergenic:Mylk3-4921524J17Rik 4:intron:Fam13b 4:intergenic:Gm13810-B230118H07Rik 4:intron:Edil3 4:intron:Cask 4:intergenic:Gm9732-Gm17155 4:intergenic:1700049E17Rik1-1700024B05Rik 4:intergenic:1700049E17Rik2-Gm17159 4:intergenic:Gm13269-Cacnb2/Gm13269 4:intron:Mut 4:intron:Haus3 4:intergenic:Adam30-Reg4 4:intergenic:Gm15213-Ap1s2 4:intergenic:Gm24288-Gm17215 3:intron:Neo1 4:intron:Lrrc18 4:intron:Cntnap2 4:intergenic:Sgk3-Gm22607 4:intergenic:Lpar4-P2ry10 4:intron:Fbxl7 3:intergenic:Ccdc71l-Nampt 4:exon:Tnrc6a 4:intron:Tmem232 3:intron:Abi3bp 4:intergenic:9330185C12Rik-Cntnap5a 4:intergenic:Olfr1147-ps1-Olfr1148 4:intergenic:Masp1-Rtp4 4:intergenic:Anxa10-Gm22324 4:intron:Fars2 4:intergenic:Dnah5-Mir3964 4:intergenic:Gm24680-4921530L21Rik 4:intergenic:Gm15501-Gm5899 4:intergenic:Gm8186-Tmem8 4:intergenic:Gm26559-Gm24471 4:intergenic:Olfr382-Olfr383-ps1 4:intergenic:Pcsk2-Gm12360/Pcsk2 4:intron:Syn3 4:intergenic:Gm24104-Gm26058 4:intergenic:Diras2-Syk 4:intron:Rb1 2:intergenic:Gm26106-Gm7729 4:intergenic:Fam19a2-Gm23777 2:intron:Dkk2 2:intergenic:Crbn-Gm22418 4:intergenic:Gm26687-Gm15453 4:intergenic:Gm15702-Bai3 2:intergenic:Gm13763-Olfr1212 4:intergenic:BB218582-Mpped2 4:intron:Acsf3 4:intergenic:Tcf21-Eya4 3:intron:Ercc4 4:intergenic:Gm22087-5S_rRNA 4:intron:Unc13c 4:intergenic:Gm23385-Gm7803 4:intergenic:Cntn6-Gm23339 4:intergenic:Gm26354-Gm7536 4:intergenic:Ttc23-Synm 4:intron:Pcdh7 4:intergenic:4933402D24Rik-Gm13751 4:intergenic:Gm26561-Myom1 4:intergenic:Gabra3-Mir767 4:intron:a 4:intergenic:Speer2-Gbe1 4:intergenic:Gm8036-Il1rapl2 3:intergenic:Gm17732-Spink13 4:intron:C030047K22Rik 4:intergenic:Gm8261-Gm15075 4:intergenic:Dmrta2-Elavl4 4:intron:Cadps2 4:exon:Nap1l1 4:intergenic:Elavl2-Gm12668 4:intergenic:Gm25709-Cpm 4:intergenic:Gm23437-Gm24818 4:intergenic:0610040J01Rik-Rell1 4:intergenic:Gm23986-Gm24929 4:intergenic:Prl7d1-Prl7c1 4:intergenic:Lphn3-Mir1187 4:intron:Ablim1 3:intergenic:Gm23594-Il20rb 3:intergenic:Zcchc16-Gm26024 4:intergenic:Dlx2-Gm1631 4:intergenic:Prl4a1-Prl5a1 4:intergenic:Gm9436-Gm5755 4:intergenic:Gm5909-Gypa 4:intron:Pfkp 4:intron:Arl15 4:intergenic:Gm11890-Gm11892 4:intergenic:A830035A12Rik/Gm11649-A830035A12Rik/Helz 4:intergenic:Egr4-Alms1 4:intergenic:Gm20475-Slit2 4:intergenic:Rock1-Greb1l 4:intron:Ppp1r9a 4:intergenic:Gm22483-Prelid2 4:intergenic:Brinp3-Gm9931 4:intron:Ctnnd2 4:intergenic:Vmn2r58-Vmn2r-ps57 4:intergenic:Gm23727-Gm20594 4:intergenic:Zfp931-Phactr3 4:intergenic:Plod2-Gm9621 3:intergenic:Gm24363-Gm12729 4:intergenic:Gm26876-Suox 3:intergenic:Gm11381-Mylk4 4:intron:Cpne4 4:intergenic:Gm12297-Gm12298 4:exon:Pigl 4:intergenic:Gpa33-Mael 4:intergenic:5033403F01Rik-Gm23972 4:intron:Pcsk5 4:intron:Reck 4:intergenic:Gm11260-Gm11261 4:intergenic:Olfr1491-Olfr1494 4:intergenic:Slitrk1-Gm25901 4:intergenic:4930594M17Rik-Gm26012 4:intergenic:Bmper-Npsr1 3:intron:Plxna2 4:intergenic:Insm1-Ralgapa2 4:intergenic:Gm17192-Gm24164 3:intron:Grm3 3:intron:Zfhx4 4:intron:Zfp438 4:intergenic:Megf9-Gm11227 3:intron:Vmn2r57/Gm20449 4:intergenic:AC158354.1-Gm4804 4:intergenic:Nav3-E2f7 4:intergenic:Gm12380-Gm12381 4:intron:Dmd 4:exon:Gpcpd1 4:intergenic:Gm25096-Gm25634 4:intron:Gm11335 3:intergenic:1700028K03Rik-A830010M20Rik/1700028K03Rik 4:intron:Mtch2 3:intergenic:Gm17377-St6gal2 4:intron:A730090N16Rik 4:intergenic:Olfr111-Olfr112 4:intron:Alk 4:intergenic:Rprl2-Gm26354 4:intron:Slc25a24 2:intergenic:Immp2l-Gm25497 4:intergenic:Gm25935-Gm22593 4:intron:Usp47 4:intron:Gabpb2 4:intergenic:Bbs9-Bmper 4:intron:Tmprss11f 4:intron:Dok5 4:intron:Aff2 3:intron:Fam124a 4:exon:Erf 4:intron:Ift140 4:intron:Apoo 3:intergenic:Gm14038-Plcb1 4:intron:Utrn 4:intron:Chst7 4:intergenic:Zfhx3-D030068K23Rik 4:intergenic:Gm22917-Gm26245 4:intron:Plce1 4:intergenic:G930045G22Rik-Mir148a 3:intergenic:Gm8036-Il1rapl2 4:intergenic:Kalrn-Ropn1 4:intergenic:Tceal3-Gm15010 4:intron:Arpp21 4:intron:Frmpd4 3:intergenic:Gm25383-Gm24863 4:exon:Cdyl2 4:intergenic:Gm22225-Gm14986 4:intron:Dennd1b 4:intergenic:Gm7493-Sntg1 4:intergenic:Gm16542-Fhit Off-target primers show all... show less...
672 / rev	`CTTTATCTAGAATATATATC AGG` `.................... ...` Low GC content Enzymes: BbsI, Hpy188III, MboII Cloning / PCR primers	26	78	45	28	66	79	0 - 3 - 2 - 49 - 213 0 - 2 - 0 - 3 - 7 267 off-targets	3:intergenic:Gm22521-Gm25702 1:intergenic:Gm7224-Vmn2r59 1:intergenic:Gm5594-Vmn2r-ps59 4:intergenic:Brd7-Nkd1 4:intergenic:Gm24948-Nova1 3:intergenic:Gm9578-Gm22891 3:intergenic:Gm25293-Cdh7 4:intron:Vmn2r58 4:intron:Nkain1 4:intron:Wdr96 4:intergenic:Gm11986-U7 4:intergenic:Ubash3b-Gm25401 4:intron:Cldn2 4:intron:Cmss1 4:intron:Rbbp5 4:intron:Kif16b 4:intergenic:Zfp109-Zfp108 4:intron:B230118H07Rik 4:intergenic:Commd4-Trcg1 4:intergenic:Gm23906-Vgll2 4:intergenic:Haus3-Gm25918 4:intergenic:4930596D02Rik-Gm7853 4:intron:Skint6 4:intergenic:Gm23661-Gm23067 4:intron:Meis2 4:intron:Shroom2 4:intergenic:Mark1-Rab3gap2 3:intron:Rnf165 4:intergenic:Gm6644-Plcl1 4:intergenic:Gm14005-Gm14010 3:intron:Prkd1 4:intergenic:Tex29-A230072I06Rik 4:intergenic:Ttc3-Dscr3 3:intron:Vrk1 4:intergenic:Gm24382-Mbnl1 4:intergenic:Rdh14-Pgk1-rs7 4:intron:Cdh8 4:intergenic:Gm26106-Gm7729 4:intron:Arhgap6 4:intergenic:Thnsl2-Fabp1 4:intergenic:Gm25086-Cntn5 4:intergenic:Gm26930-Gm26936 3:intron:App 4:intergenic:Nox3-Gm23186 4:intergenic:Gm7846-Gm597 4:intron:Eif3h 4:intergenic:Zmynd12-Rimkla/Zmynd12 4:intron:Zfp362 4:intergenic:Gm25382-Cited2 4:intron:Pcsk1 3:intergenic:AC166813.1-2610316D01Rik 4:intron:Pard3b 4:intergenic:Rtkn2-Arid5b 4:intron:Dpysl2 3:intergenic:Acot10-Gm25711 3:intergenic:Gm13255-Gm13217 4:intergenic:Gm23860-Gm11800 4:intron:Skint6 4:intron:Dlgap2 4:intron:Lphn3 4:intergenic:Adamts1-Adamts5 4:intergenic:Cckar-Tbc1d19 4:intron:Vps13b 4:intergenic:Ighd4-1-Ighd3-2 4:intron:Trpc6 4:intergenic:Gm6098-Zbbx 4:intergenic:Slc46a2-Mup4 4:intron:Sult2b1 4:intergenic:Gm8601-Btg1 4:intron:Rragb 4:intergenic:Gm11675-Gm11676 4:intron:Slc9a2 4:intergenic:Pcdh17-Gm23926 3:intron:Sorcs3 4:intron:Fmn1 4:intergenic:Olfr446-Olfr444 4:intergenic:Gm13479-Gm13478 4:intergenic:Gm26835-Hivep2 4:intergenic:Maf/Gm15655-Dynlrb2 4:intergenic:Gm14947-Gm14940 4:intron:Abca14 4:intergenic:Gm14032-Gm14033 4:intergenic:Slco1a4-Slco1a1 4:intergenic:Gm22273-Gm24079 4:intergenic:Gm9897-Fzd1 4:intergenic:Gm24681-Gm23591 4:intergenic:Gm16542-Fhit 4:intron:Cntnap3 4:intron:Col4a5 3:intergenic:Gm23477-Gm10780 4:intergenic:Gm5815-2610034M16Rik 3:intron:Cdh8 3:intergenic:Gm23389-Gm23181 4:intergenic:Gm26257-Gp49a/Lilrb4 4:intergenic:Gm16139-Fgd4 4:intergenic:Olfr466-Gm24130 4:intergenic:Gm16032-Mettl16 4:intron:Wdr17 3:intergenic:Cdk1-Ank3 3:intron:4930452B06Rik 4:intron:Nrxn3 4:intron:Nell1 4:intergenic:Gm7426-Nid1 4:intergenic:Gm24065-Gm2399 3:intergenic:Gm12066-Gm12068 4:intergenic:Gm12807-Agbl4 4:intron:Slc12a8 4:intergenic:Lhfp-Nhlrc3/Gm16206 4:intergenic:Snx16-Gm22795 4:intron:March11 4:intron:Lrriq1 4:intergenic:Khdrbs2-Gm23024 4:intron:Mysm1 4:intergenic:Gm22016-Cdc73 3:intergenic:Gm23203-Gm23447 4:intron:Hexa 4:intron:Pard3 4:intergenic:Gm24432-Gm23411 4:intergenic:Gm15000-Gm15001 4:intergenic:Gucy1a3-Map9 3:intergenic:Grpr-Rnf138rt1 3:intergenic:Gm9956-Gm24285 4:intergenic:Gm24546-Olfr24 3:intron:Rundc3b 3:intron:Cntn5 4:intergenic:Gm9843-Usp25 4:intergenic:Spr-ps1-Emx1 4:intergenic:Lrrc7-Gm9423 3:intergenic:Gm24736-Alk 4:intergenic:Gm13623-Ccdc173 4:intron:Gpr98 4:intergenic:Nmd3-Sptssb 4:intergenic:Gm23520-Gm12131 4:intron:Ccdc132 3:intergenic:Dmrt2-Gm8825 4:intergenic:Gm12717-Dab1 2:intron:Skint6 4:intergenic:Tprg-Trp63 4:intergenic:Ktn1-Gm6055 4:intergenic:Zfp825-Erap1 4:intergenic:D19Ertd737e/4933412A08Rik-Prlhr 4:intron:Ralgps2 4:intron:Slc39a8 4:intron:Xylt1 4:intergenic:Cyp7b1-Gm6369 4:intron:Efhc2 4:intron:Mipep 3:intergenic:Gm26639-Gm26758 3:intergenic:AC164297.1-Zfp640 3:intergenic:Gm10324-Gm17514 3:intergenic:Gm26639-Gm26758 3:intergenic:Gm26754-Gm26715 3:intergenic:Gm26754-Gm26715 3:intergenic:Gm5792-Gm10139 3:intergenic:Zfp640-Gm17404 3:intergenic:Zfp369-Gm5792 3:intergenic:Gm26715-2410141K09Rik 3:intergenic:Gm6917-Gm6910 3:intergenic:Gm10139-Gm6901 3:intergenic:Gm26806-AC164297.1 3:intergenic:Gm6917-Gm6910 3:intergenic:Zfp369-Gm5792 3:intergenic:Gm10323-Gm5196 3:intergenic:Zfp640-Gm17404 3:intron:2410141K09Rik 4:intron:Phf21a 4:intergenic:Mep1a-Ankrd66 4:intron:Pias1 4:intergenic:Edil3-Gm26120 4:intergenic:Gm10309-Prkce 3:intergenic:Gm7237-Gm8975 4:intergenic:Gm23022-Rab28 4:intron:Nol4 4:intron:Hgd 4:intergenic:Gm25602-Tbc1d32 4:intergenic:Emx2-Gm17203 4:intergenic:Gm14071-Sptlc3 3:intron:Spr 4:intergenic:Hey2-Gm24168 4:intergenic:Ppef1-Rs1 4:intergenic:Gm23900-Zfp804a 4:intergenic:Gm11889-Gm25978 4:intergenic:Gm24250-Nlgn1 4:intergenic:Fam174a-Gm15427 4:intron:Adamts16 4:intergenic:Ccdc50-Fgf12 4:intergenic:Gm23595-Dock3 4:intergenic:Gm26076-Gm25519 4:intergenic:Gm25202-Gm14505 4:intergenic:Gm13364-Gm6587 4:intergenic:Rarb-Thrb 4:intergenic:Gm20641-Nrg3 2:intergenic:Gm6027-Gm4746 4:exon:Sel1l3 4:intron:Col6a4 4:intergenic:Gm8692-Gm14710 4:intergenic:A630076J17Rik-Prok1 4:intergenic:Gm23038-4930570G19Rik 3:intron:Impg2 3:intergenic:Gm22104-Gm25550 4:intergenic:Rnpc3-Col11a1 4:intergenic:Wdr17-n-R5s98 1:intergenic:Gm5593-Vmn2r60 4:intron:Ankrd28 4:intergenic:Gm22886-Klhl14 4:intergenic:Gm5135-Xiap 4:intergenic:Gm7665-Cdh2 4:intron:Grin2a 4:intergenic:Gm8100-Gm26795 4:intergenic:Gm18355-Gm4745 4:intergenic:Olfr1117-ps1-Gm13729 4:intron:Zfp715 4:intergenic:Ikzf2-Spag16 4:intron:A930029G22Rik 4:intergenic:Gm11498-Gm22702 3:intergenic:Agtr1b-Gm7488 4:intergenic:Gm5519-Gm8988 4:intron:Mgat4c 4:intergenic:Dennd2a-Dennd2a/8030453O22Rik 4:intron:Abca1 4:intron:Npas3 4:intron:Setd2 4:intergenic:Gm23447-Gm23773 4:intergenic:RP23-448C3.1/Trpm3-Trpm3 4:intergenic:Gltscr1-Zfp541 3:intergenic:Gm26245-Gm26078 4:intron:Cntnap5c 3:intergenic:n-R5s60-Gm26256 4:intergenic:Gm11659-Mapt 4:intron:Zfp618 4:intron:Pepd 4:intergenic:Pcdh17-Gm23926 4:intergenic:Samd12-Gm23200 4:intergenic:Ap5b1-Rnaseh2c 4:intergenic:Gm16294-Zfpm2 4:intergenic:Gm6327-Ercc4 4:intergenic:Gm24177-Gm12484 4:intron:Arf2 4:intergenic:Gpr101-Gm14630 4:intergenic:Gm26489-1700028P15Rik 4:exon:Ing3 4:exon:4930510E17Rik 4:intergenic:Slitrk1-Gm25901 4:intron:Bbox1 4:intron:Zfand6 4:intergenic:Racgap1-Asic1 4:intergenic:Gm23796-Gm23831 4:intergenic:Gm22866-Gm5620 4:intergenic:Gm22011-Mthfr-ps1 4:intergenic:Gm20642-Nrg3 4:intron:Asah2 4:intergenic:Gm14970-4930412D23Rik 4:intron:Pcsk5 4:intergenic:Gm24213-mmu-mir-6370 4:intron:Arap2 4:intergenic:Rpl36-ps2-Abr 4:intergenic:Gm6489-Gm10307 4:intergenic:Gm26480-Gm6863 4:intergenic:Gm8991-Gm6673 4:intergenic:4930449E01Rik-mmu-mir-6390 4:intergenic:Gm21614-Gm23249 4:intron:Stxbp5 4:intergenic:Anpep-Ap3s2 4:intron:Gm765 4:intergenic:Csnka2ip-4930453N24Rik 4:intron:Fam49a 4:intergenic:Gm5123-Dusp21 Off-target primers show all... show less...
33 / rev	`ATTTTAAAGACAAGGGGCTG TGG` `.................... ...` Not with U6/U3 Cloning / PCR primers	21	46	51	26	69	75	0 - 2 - 15 - 218 - 587 0 - 1 - 0 - 6 - 11 822 off-targets	More than 500 off-targets, showing 13 with score >2.0 2:intron:Gm12162 2:intergenic:Gm807-Zfp366 2:intergenic:Gm3435-Tcte3 2:intergenic:Gm23434-Cntnap5b 2:intergenic:Gm23681-Peli1 2:intron:Dnah7a 2:intron:Ermard 2:exon:9030025P20Rik 2:intergenic:Slc30a10-Lyplal1 2:intergenic:Gm24670-Etl4 1:intron:Tspan18 2:intergenic:Gm22741-Rps10-ps2 1:intergenic:Gm22949-3632451O06Rik Off-target primers show all... show less...

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